AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380012544.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380012544.01.T01 MTR_3g026310 88.419 449 52 0 1 449 1 449 0.0 824
MsG0380012544.01.T01 MTR_3g088420 72.606 449 108 3 1 449 1 434 0.0 673
MsG0380012544.01.T01 MTR_4g022825 66.741 448 141 3 1 448 1 440 0.0 612
MsG0380012544.01.T01 MTR_4g022800 66.147 449 146 2 1 449 1 443 0.0 607
MsG0380012544.01.T01 MTR_4g022720 66.147 449 139 3 1 449 1 436 0.0 598
MsG0380012544.01.T01 MTR_4g022670 64.811 449 151 2 1 449 1 442 0.0 593
MsG0380012544.01.T01 MTR_7g026860 64.588 449 149 2 1 449 1 439 0.0 591
MsG0380012544.01.T01 MTR_4g022760 59.020 449 133 2 1 449 1 398 0.0 523
MsG0380012544.01.T01 MTR_8g041770 56.522 460 171 6 1 449 1 442 3.46e-179 508
MsG0380012544.01.T01 MTR_0190s0020 55.773 459 175 7 1 448 1 442 9.62e-174 494
MsG0380012544.01.T01 MTR_4g094842 55.411 462 170 11 1 444 1 444 3.22e-168 480
MsG0380012544.01.T01 MTR_4g094835 51.515 462 186 10 1 444 1 442 5.65e-159 457
MsG0380012544.01.T01 MTR_4g094828 51.398 465 194 11 1 448 1 450 5.60e-152 439
MsG0380012544.01.T01 MTR_8g079710 50.649 462 189 12 1 444 1 441 1.84e-148 430
MsG0380012544.01.T01 MTR_6g016815 52.212 452 129 9 1 449 15 382 3.64e-143 414
MsG0380012544.01.T01 MTR_4g022290 49.251 467 190 13 1 445 1 442 1.46e-141 412
MsG0380012544.01.T01 MTR_4g094838 48.060 464 182 10 4 444 1 428 6.85e-140 408
MsG0380012544.01.T01 MTR_4g094832 52.051 390 153 7 69 444 57 426 2.99e-133 391
MsG0380012544.01.T01 MTR_8g040760 64.977 217 69 3 1 211 1 216 4.75e-93 280
MsG0380012544.01.T01 MTR_3g101900 41.190 437 227 12 24 448 30 448 2.77e-92 286
MsG0380012544.01.T01 MTR_3g101930 39.326 445 229 11 24 447 30 454 4.91e-89 278
MsG0380012544.01.T01 MTR_3g102020 38.149 443 236 11 24 448 29 451 3.12e-86 271
MsG0380012544.01.T01 MTR_3g101970 37.955 440 225 11 24 447 29 436 1.93e-85 268
MsG0380012544.01.T01 MTR_3g102010 38.009 442 237 12 24 448 30 451 1.25e-81 259
MsG0380012544.01.T01 MTR_3g101960 37.931 435 237 11 24 443 29 445 2.87e-81 258
MsG0380012544.01.T01 MTR_8g040780 56.221 217 89 3 228 440 10 224 7.82e-77 240
MsG0380012544.01.T01 MTR_7g026850 68.790 157 49 0 29 185 12 168 1.94e-75 234
MsG0380012544.01.T01 MTR_4g015180 34.091 484 253 16 17 449 20 488 8.05e-65 216
MsG0380012544.01.T01 MTR_5g081960 32.558 473 262 13 22 448 25 486 9.61e-65 216
MsG0380012544.01.T01 MTR_3g070170 30.932 472 290 13 2 448 10 470 6.54e-62 208
MsG0380012544.01.T01 MTR_7g026770 55.897 195 53 2 141 335 132 293 2.75e-58 194
MsG0380012544.01.T01 MTR_4g094825 56.287 167 66 4 16 176 83 248 1.79e-55 189
MsG0380012544.01.T01 MTR_4g094825 48.101 158 67 3 289 444 243 387 8.68e-39 144
MsG0380012544.01.T01 MTR_6g034495 64.800 125 41 1 64 185 43 167 4.02e-50 168
MsG0380012544.01.T01 MTR_3g102030 35.202 321 161 9 87 404 44 320 1.03e-49 172
MsG0380012544.01.T01 MTR_6g045553 28.970 466 298 10 4 444 14 471 3.07e-48 172
MsG0380012544.01.T01 MTR_6g034485 58.696 138 44 3 188 325 1 125 6.06e-47 158
MsG0380012544.01.T01 MTR_7g026830 70.588 85 25 0 82 166 14 98 4.52e-37 131
MsG0380012544.01.T01 MTR_4g007540 25.877 456 277 15 18 449 14 432 3.79e-32 127
MsG0380012544.01.T01 MTR_8g075610 25.871 402 244 15 55 437 47 413 8.78e-31 123
MsG0380012544.01.T01 MTR_7g026800 42.188 192 68 7 211 395 18 173 2.57e-29 114
MsG0380012544.01.T01 MTR_3g026950 27.407 405 232 16 30 400 28 404 5.22e-29 119
MsG0380012544.01.T01 MTR_8g096530 28.502 414 238 15 55 441 59 441 2.64e-28 116
MsG0380012544.01.T01 MTR_4g022780 40.415 193 76 7 200 389 7 163 1.46e-27 108
MsG0380012544.01.T01 MTR_3g049150 28.205 429 248 16 1 399 1 399 2.16e-27 114
MsG0380012544.01.T01 MTR_2g105330 25.693 397 240 13 55 430 56 418 5.47e-27 112
MsG0380012544.01.T01 MTR_3g098980 28.125 384 236 14 55 429 66 418 2.13e-26 111
MsG0380012544.01.T01 MTR_2g064495 26.250 400 234 16 55 430 56 418 3.25e-26 110
MsG0380012544.01.T01 MTR_4g097090 25.481 416 264 15 55 447 65 457 3.74e-26 110
MsG0380012544.01.T01 MTR_7g014250 28.103 427 252 16 14 406 11 416 1.42e-24 106
MsG0380012544.01.T01 MTR_7g026780 46.903 113 34 1 337 449 26 112 2.04e-24 97.8
MsG0380012544.01.T01 MTR_7g014330 27.778 432 256 19 6 406 10 416 3.64e-23 102
MsG0380012544.01.T01 MTR_3g087640 25.607 453 289 13 17 435 24 462 1.62e-21 97.1
MsG0380012544.01.T01 MTR_7g014230 27.083 432 259 18 6 406 12 418 2.99e-21 96.3
MsG0380012544.01.T01 MTR_6g015815 24.130 460 280 18 15 437 25 452 3.31e-21 96.3
MsG0380012544.01.T01 MTR_4g127650 23.799 458 290 18 21 447 28 457 2.85e-20 93.2
MsG0380012544.01.T01 MTR_2g089670 26.420 405 255 13 21 399 42 429 2.87e-20 93.6
MsG0380012544.01.T01 MTR_7g027175 26.329 395 254 14 50 435 63 429 3.00e-20 93.2
MsG0380012544.01.T01 MTR_7g014160 24.268 478 281 17 3 429 8 455 4.69e-20 92.8
MsG0380012544.01.T01 MTR_4g013050 24.796 367 201 12 54 400 52 363 5.68e-20 92.0
MsG0380012544.01.T01 MTR_6g015320 25.559 313 207 11 138 435 143 444 1.01e-19 91.7
MsG0380012544.01.T01 MTR_2g089765 22.799 443 288 12 24 429 39 464 1.32e-19 91.7
MsG0380012544.01.T01 MTR_6g015315 27.414 321 198 13 138 438 140 445 2.19e-19 90.5
MsG0380012544.01.T01 MTR_1g081660 25.187 401 241 16 24 400 31 396 3.44e-19 90.1
MsG0380012544.01.T01 MTR_3g028210 23.276 464 297 16 4 430 8 449 3.47e-19 90.1
MsG0380012544.01.T01 MTR_8g012750 26.174 447 268 18 3 406 2 429 7.30e-19 89.4
MsG0380012544.01.T01 MTR_5g026320 25.908 413 250 17 52 443 71 448 8.70e-19 89.0
MsG0380012544.01.T01 MTR_3g086020 26.121 379 233 13 55 429 61 396 1.25e-17 85.1
MsG0380012544.01.T01 MTR_1g053315 24.450 409 268 17 55 444 78 464 1.91e-17 84.7
MsG0380012544.01.T01 MTR_2g089650 23.904 456 287 17 7 425 11 443 3.30e-17 84.0
MsG0380012544.01.T01 MTR_6g015830 24.401 459 289 17 10 437 21 452 3.74e-17 84.0
MsG0380012544.01.T01 MTR_2g089735 24.105 419 270 14 38 425 40 441 9.18e-17 82.8
MsG0380012544.01.T01 MTR_3g049140 28.342 187 106 6 233 400 20 197 1.13e-16 80.1
MsG0380012544.01.T01 MTR_7g114520 23.691 401 248 16 25 400 25 392 1.50e-16 82.0
MsG0380012544.01.T01 MTR_7g014360 28.470 281 165 7 138 406 90 346 1.54e-16 81.6
MsG0380012544.01.T01 MTR_7g026870 60.000 55 22 0 302 356 97 151 1.95e-16 76.6
MsG0380012544.01.T01 MTR_6g015850 24.622 463 280 19 10 437 21 449 9.53e-16 79.7
MsG0380012544.01.T01 MTR_5g090810 25.500 400 244 17 32 400 36 412 1.61e-15 79.0
MsG0380012544.01.T01 MTR_7g026880 63.462 52 19 0 397 448 2 53 1.66e-15 70.9
MsG0380012544.01.T01 MTR_4g055170 26.056 284 184 8 55 330 69 334 1.71e-15 79.0
MsG0380012544.01.T01 MTR_7g114550 22.870 446 281 19 25 440 24 436 3.31e-15 77.8
MsG0380012544.01.T01 MTR_7g014200 23.340 467 296 13 2 429 7 450 3.59e-15 77.8
MsG0380012544.01.T01 MTR_5g084870 23.131 428 286 13 4 421 3 397 4.42e-15 77.4
MsG0380012544.01.T01 MTR_5g090830 25.750 400 243 17 32 400 36 412 8.08e-15 76.6
MsG0380012544.01.T01 MTR_6g053810 26.667 330 201 13 49 364 65 367 1.17e-14 76.3
MsG0380012544.01.T01 MTR_3g112180 23.869 398 268 12 48 435 54 426 1.58e-14 75.9
MsG0380012544.01.T01 MTR_7g086000 25.432 405 259 17 58 437 63 449 8.50e-14 73.6
MsG0380012544.01.T01 MTR_7g014610 24.124 485 269 21 22 449 27 469 1.14e-13 73.2
MsG0380012544.01.T01 MTR_3g071320 23.342 407 263 12 54 444 59 432 1.18e-13 72.8
MsG0380012544.01.T01 MTR_2g015430 24.079 353 233 12 58 402 61 386 1.32e-13 72.8
MsG0380012544.01.T01 MTR_7g056087 22.051 390 279 10 54 435 57 429 1.82e-13 72.4
MsG0380012544.01.T01 MTR_1g094790 26.136 440 265 18 29 446 38 439 4.34e-13 71.2
MsG0380012544.01.T01 MTR_3g465990 24.214 318 206 12 55 364 68 358 5.52e-13 70.9
MsG0380012544.01.T01 MTR_3g465930 27.148 291 181 10 50 329 64 334 1.01e-12 70.1
MsG0380012544.01.T01 MTR_3g465910 27.385 325 197 15 50 364 63 358 1.80e-12 69.7
MsG0380012544.01.T01 MTR_0502s0010 29.717 212 109 8 134 330 39 225 2.46e-12 67.0
MsG0380012544.01.T01 MTR_7g014620 23.557 433 271 12 24 429 15 414 3.63e-12 68.6
MsG0380012544.01.T01 MTR_7g014310 27.972 143 97 2 264 406 147 283 7.03e-12 66.6
MsG0380012544.01.T01 MTR_7g014670 28.966 145 94 3 287 429 104 241 2.71e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380012544.01.T01 AT5G67150 40.821 463 240 12 1 447 1 445 3.15e-104 317
MsG0380012544.01.T01 AT3G50300 42.857 441 223 12 1 429 1 424 1.63e-103 315
MsG0380012544.01.T01 AT3G50280 40.263 457 244 10 1 445 1 440 7.70e-103 313
MsG0380012544.01.T01 AT5G67160 40.133 451 238 12 3 448 9 432 3.74e-98 301
MsG0380012544.01.T01 AT3G50270 38.126 459 254 12 4 448 6 448 2.99e-93 289
MsG0380012544.01.T01 AT5G38130 38.636 440 241 10 1 429 21 442 2.85e-90 282
MsG0380012544.01.T01 AT5G42830 37.862 449 244 12 15 448 21 449 5.19e-87 273
MsG0380012544.01.T01 AT5G07860 36.069 463 259 11 2 447 6 448 1.19e-79 254
MsG0380012544.01.T01 AT5G07870 36.809 470 262 12 2 449 6 462 1.15e-77 249
MsG0380012544.01.T01 AT3G50280 41.590 327 168 6 125 445 1 310 2.67e-74 236
MsG0380012544.01.T01 AT2G39980 33.402 488 242 15 17 449 22 481 1.73e-69 228
MsG0380012544.01.T01 AT5G07850 34.868 456 253 14 15 449 22 454 5.97e-69 226
MsG0380012544.01.T01 AT5G01210 33.403 476 247 13 17 448 20 469 1.64e-65 218
MsG0380012544.01.T01 AT3G50290 41.608 286 143 6 119 397 1 269 2.09e-60 198
MsG0380012544.01.T01 AT5G23940 30.753 465 279 16 4 444 3 448 2.55e-53 186
MsG0380012544.01.T01 AT5G57840 27.955 440 283 15 4 429 3 422 6.14e-26 110
MsG0380012544.01.T01 AT5G48930 26.786 392 242 13 27 400 23 387 9.31e-26 109
MsG0380012544.01.T01 AT2G19070 25.727 447 289 16 27 445 19 450 1.50e-25 108
MsG0380012544.01.T01 AT3G48720 25.672 409 261 12 42 436 44 423 1.16e-24 106
MsG0380012544.01.T01 AT5G41040 27.731 357 223 12 52 400 66 395 3.16e-23 102
MsG0380012544.01.T01 AT5G41040 27.731 357 223 12 52 400 82 411 3.48e-23 102
MsG0380012544.01.T01 AT3G29590 26.247 461 293 17 2 439 6 442 1.41e-22 100
MsG0380012544.01.T01 AT5G63560 27.273 385 239 16 55 430 61 413 2.30e-21 96.3
MsG0380012544.01.T01 AT1G28680 27.247 356 227 15 58 407 65 394 5.20e-20 92.4
MsG0380012544.01.T01 AT3G26040 25.962 416 253 15 49 448 53 429 6.62e-20 92.0
MsG0380012544.01.T01 AT2G40230 27.671 365 221 14 54 405 58 392 1.07e-19 91.3
MsG0380012544.01.T01 AT3G29670 23.060 451 302 15 1 429 6 433 4.19e-19 89.7
MsG0380012544.01.T01 AT3G29635 25.173 433 278 14 22 429 27 438 1.02e-18 88.6
MsG0380012544.01.T01 AT1G03390 25.943 424 268 17 42 441 59 460 1.63e-17 85.1
MsG0380012544.01.T01 AT5G07080 26.690 281 179 10 55 327 70 331 9.17e-17 82.8
MsG0380012544.01.T01 AT1G28680 27.333 300 197 12 58 354 65 346 1.73e-16 81.3
MsG0380012544.01.T01 AT5G23970 25.123 406 268 15 49 448 46 421 3.34e-16 80.9
MsG0380012544.01.T01 AT5G61160 22.277 404 278 14 22 406 23 409 3.32e-15 77.8
MsG0380012544.01.T01 AT3G29680 23.881 402 255 14 24 400 25 400 6.62e-15 77.0
MsG0380012544.01.T01 AT1G32910 25.485 361 228 14 55 400 65 399 8.98e-15 76.6
MsG0380012544.01.T01 AT1G78990 25.203 369 241 13 43 400 52 396 1.29e-14 76.3
MsG0380012544.01.T01 AT5G39050 25.419 358 206 9 120 435 130 468 2.25e-14 75.5
MsG0380012544.01.T01 AT1G24430 25.330 379 230 16 54 421 61 397 3.14e-14 74.7
MsG0380012544.01.T01 AT1G24420 24.928 349 209 17 65 402 75 381 3.39e-13 71.6
MsG0380012544.01.T01 AT1G24420 24.928 349 209 17 65 402 75 381 3.39e-13 71.6

Find 94 sgRNAs with CRISPR-Local

Find 128 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACGACATGGTGTTGTCTTTA+TGG 0.155383 3:-19501444 None:intergenic
TCCCCGCGGTTTGATGTTTA+TGG 0.196862 3:+19502363 MsG0380012544.01.T01:CDS
GTGTCTTCCTCACAAGATTT+TGG 0.229479 3:+19502494 MsG0380012544.01.T01:CDS
CCGTTGCCGAAAGATTATTT+TGG 0.284850 3:+19502126 MsG0380012544.01.T01:CDS
GATGTTTATGGCAATAATTT+TGG 0.301289 3:+19502375 MsG0380012544.01.T01:CDS
GGTGATAAAGGAGACCCTTT+TGG 0.329078 3:-19501324 None:intergenic
TTAGTTGACGGCATCTTTAT+TGG 0.332453 3:+19501649 MsG0380012544.01.T01:CDS
GCCTGCAAGTGGTGGGAAAA+AGG 0.338259 3:-19501404 None:intergenic
ATTGAAAGCCAATGTAGAAA+TGG 0.347172 3:+19501969 MsG0380012544.01.T01:CDS
TTTAACCATCTGGTTGTTGA+TGG 0.349436 3:+19501679 MsG0380012544.01.T01:CDS
AAATGCTGTTGTAGGTTCTA+TGG 0.351981 3:+19502149 MsG0380012544.01.T01:CDS
TTGAGTGCAAAGAAATCATT+TGG 0.352198 3:-19502236 None:intergenic
GGACTGGTTGTTGTATTTGC+TGG 0.355262 3:+19502441 MsG0380012544.01.T01:CDS
ACTCTAGAGGATGGTGATAA+AGG 0.360277 3:-19501336 None:intergenic
GCATTTCCAAAATAATCTTT+CGG 0.388444 3:-19502132 None:intergenic
TGAAGAATGACTATGAGAAT+TGG 0.389153 3:+19502268 MsG0380012544.01.T01:CDS
TTACCAAGTCTAAGGATGAT+TGG 0.394784 3:-19502300 None:intergenic
TGTTACTATTACCAAGTCTA+AGG 0.399364 3:-19502308 None:intergenic
CTATGGTTGCAATGAAAGTT+GGG 0.402541 3:+19502166 MsG0380012544.01.T01:CDS
AATAGCCAACACACGATAAT+CGG 0.404923 3:-19502334 None:intergenic
ACGTTTAAGTGTTGAGAGTT+CGG 0.410636 3:-19501770 None:intergenic
CCTCTCTACAGATACAATAA+AGG 0.428765 3:+19501231 MsG0380012544.01.T01:CDS
ATGGTATTTCATTAATTCGT+GGG 0.435786 3:+19501711 MsG0380012544.01.T01:CDS
GTTGGTACCCATTTCTACAT+TGG 0.457489 3:-19501977 None:intergenic
TTCTTGCGGGTCTAATTGGT+CGG 0.459425 3:-19502055 None:intergenic
TCTATGGTTGCAATGAAAGT+TGG 0.459986 3:+19502165 MsG0380012544.01.T01:CDS
ACTTGTTGAACCCACCCAAA+AGG 0.461769 3:+19501309 MsG0380012544.01.T01:CDS
GAAGAATGACTATGAGAATT+GGG 0.462181 3:+19502269 MsG0380012544.01.T01:CDS
TGGAATAGAAGAAGGTAGTA+TGG 0.465384 3:+19502461 MsG0380012544.01.T01:CDS
GTTGGGGAGTTGTTGGAAGA+AGG 0.465812 3:+19502183 MsG0380012544.01.T01:CDS
AAGCACTTTATGCACACCTT+TGG 0.465909 3:+19502016 MsG0380012544.01.T01:CDS
CTTAATGAAGGGAAATCGTA+TGG 0.467781 3:-19501818 None:intergenic
TTGAAAGCCAATGTAGAAAT+GGG 0.474020 3:+19501970 MsG0380012544.01.T01:CDS
ATTGAGAATGGGTGGAATAT+AGG 0.483367 3:-19501551 None:intergenic
CCAAAATAATCTTTCGGCAA+CGG 0.489772 3:-19502126 None:intergenic
CGAATTTGGTTTGACTCTAG+AGG 0.489830 3:-19501349 None:intergenic
AACTCTCAACACTTAAACGT+TGG 0.498070 3:+19501773 MsG0380012544.01.T01:CDS
AATGAAAGTTGGGGAGTTGT+TGG 0.508990 3:+19502176 MsG0380012544.01.T01:CDS
ATGGGACGTTGAATACCATC+TGG 0.510725 3:-19501799 None:intergenic
GTATTTGCTGGAATAGAAGA+AGG 0.513089 3:+19502453 MsG0380012544.01.T01:CDS
CTTGTTGAACCCACCCAAAA+GGG 0.515508 3:+19501310 MsG0380012544.01.T01:CDS
TGTGAGGAAGACACACTTGA+AGG 0.517501 3:-19502485 None:intergenic
TGCCATAAACATCAAACCGC+GGG 0.519171 3:-19502365 None:intergenic
AGCGGATCACGGAGCACCAA+AGG 0.521379 3:-19502032 None:intergenic
CGCAGTGTAAATAATATAAG+TGG 0.523615 3:+19502420 MsG0380012544.01.T01:CDS
TTTACACTGCGAACCGCCAC+AGG 0.524757 3:-19502408 None:intergenic
TGTAAATAATATAAGTGGAC+TGG 0.528452 3:+19502425 MsG0380012544.01.T01:CDS
TTGTATCTGTAGAGAGGACT+CGG 0.529270 3:-19501225 None:intergenic
GGTGTGCATAAAGTGCTTGA+AGG 0.530480 3:-19502011 None:intergenic
TATGGTCTATTATTTCTAGA+CGG 0.532063 3:-19501426 None:intergenic
TCTAGACGGCCTGCAAGTGG+TGG 0.533401 3:-19501412 None:intergenic
AAATGAAATAACCTCTCTGA+TGG 0.539126 3:-19501919 None:intergenic
TATGGTATTTCATTAATTCG+TGG 0.548533 3:+19501710 MsG0380012544.01.T01:CDS
TTGTTGGAAGAAGGCGCACT+TGG 0.557654 3:+19502192 MsG0380012544.01.T01:CDS
TCTTACTCCATGGGATCTCA+AGG 0.561820 3:+19501282 MsG0380012544.01.T01:CDS
CATTGCCGATTATCGTGTGT+TGG 0.567779 3:+19502329 MsG0380012544.01.T01:CDS
ACCAGCATTGTTGCATGTGA+CGG 0.568355 3:-19501470 None:intergenic
TACAAAAGAACAAATCTGAC+GGG 0.570323 3:+19501845 MsG0380012544.01.T01:CDS
TAATTGGTCGGAGCGGATCA+CGG 0.571907 3:-19502043 None:intergenic
CTTAAACGTTGGTTTCCAGA+TGG 0.571951 3:+19501784 MsG0380012544.01.T01:CDS
GTGACTTGCACCGCAAGCAA+TGG 0.572723 3:-19501622 None:intergenic
AATCCAATCATCCTTAGACT+TGG 0.582141 3:+19502297 MsG0380012544.01.T01:CDS
TTTGGTTTGACTCTAGAGGA+TGG 0.583760 3:-19501345 None:intergenic
CAAGTCACAGAGTTAGTTGA+CGG 0.585682 3:+19501637 MsG0380012544.01.T01:CDS
TCTGGTTGTTGATGGAACTT+CGG 0.586479 3:+19501687 MsG0380012544.01.T01:CDS
GTGTGCATAAAGTGCTTGAA+GGG 0.587242 3:-19502010 None:intergenic
AGTAGAACCTTGAGATCCCA+TGG 0.591120 3:-19501289 None:intergenic
CCTTTATTGTATCTGTAGAG+AGG 0.600283 3:-19501231 None:intergenic
CTACAAAAGAACAAATCTGA+CGG 0.603675 3:+19501844 MsG0380012544.01.T01:CDS
ATTTCTAGACGGCCTGCAAG+TGG 0.609454 3:-19501415 None:intergenic
ACTTGTGTAGGTGACATACT+TGG 0.616854 3:+19501523 MsG0380012544.01.T01:CDS
TCCGTCACATGCAACAATGC+TGG 0.617050 3:+19501469 MsG0380012544.01.T01:CDS
ATCTGTAGAGAGGACTCGGA+TGG 0.618089 3:-19501221 None:intergenic
AAATAATCTTTCGGCAACGG+TGG 0.618582 3:-19502123 None:intergenic
TATGGTTGCAATGAAAGTTG+GGG 0.623366 3:+19502167 MsG0380012544.01.T01:CDS
CTAGACGGCCTGCAAGTGGT+GGG 0.623531 3:-19501411 None:intergenic
TTGTTGATGGAACTTCGGTA+TGG 0.625998 3:+19501692 MsG0380012544.01.T01:CDS
AATGAAATAACCTCTCTGAT+GGG 0.633018 3:-19501918 None:intergenic
GAGGTTATTTCATTTCACAA+AGG 0.635344 3:+19501927 MsG0380012544.01.T01:CDS
TGGATGGGGAAAACCTGTGG+CGG 0.653437 3:+19502395 MsG0380012544.01.T01:CDS
TTAATGAAGGGAAATCGTAT+GGG 0.656726 3:-19501817 None:intergenic
AAGTTCCATCAACAACCAGA+TGG 0.663833 3:-19501684 None:intergenic
GCATGTGACGGAACACGACA+TGG 0.665285 3:-19501458 None:intergenic
ATTGCCTCCAAAATCTTGTG+AGG 0.672285 3:-19502501 None:intergenic
GCAATTGAGAACACTTGTGT+AGG 0.675928 3:+19501511 MsG0380012544.01.T01:CDS
AAGTGTTCTCAATTGCAGCA+TGG 0.683875 3:-19501504 None:intergenic
TTGCCATAAACATCAAACCG+CGG 0.691006 3:-19502366 None:intergenic
GCGGGTCTAATTGGTCGGAG+CGG 0.694903 3:-19502050 None:intergenic
TCTCGATAGGAACTAGACAA+AGG 0.701206 3:+19502094 MsG0380012544.01.T01:CDS
TCGTGGGCTGAAATCTCACG+TGG 0.711403 3:+19501727 MsG0380012544.01.T01:CDS
GCCATAAACATCAAACCGCG+GGG 0.723500 3:-19502364 None:intergenic
TGGGCTGAAATCTCACGTGG+TGG 0.725908 3:+19501730 MsG0380012544.01.T01:CDS
TACGTCACAACCATTGCTTG+CGG 0.749048 3:+19501612 MsG0380012544.01.T01:CDS
TGGCTATTAGCAGTTCCCCG+CGG 0.755353 3:+19502349 MsG0380012544.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTCATCAGTTGAATT+TGG - Chr3:19501259-19501278 None:intergenic 20.0%
! AAAAAATTGATCTTACTCCA+TGG + Chr3:19501272-19501291 MsG0380012544.01.T01:CDS 25.0%
! AAAAATTGATCTTACTCCAT+GGG + Chr3:19501273-19501292 MsG0380012544.01.T01:CDS 25.0%
! ATGGTATTTCATTAATTCGT+GGG + Chr3:19501711-19501730 MsG0380012544.01.T01:CDS 25.0%
! GCATTTCCAAAATAATCTTT+CGG - Chr3:19502135-19502154 None:intergenic 25.0%
! GGAAAAAATGAATTGAGAAT+GGG - Chr3:19501565-19501584 None:intergenic 25.0%
! TATGGTATTTCATTAATTCG+TGG + Chr3:19501710-19501729 MsG0380012544.01.T01:CDS 25.0%
! TGGAAAAAATGAATTGAGAA+TGG - Chr3:19501566-19501585 None:intergenic 25.0%
! TGTAAATAATATAAGTGGAC+TGG + Chr3:19502425-19502444 MsG0380012544.01.T01:CDS 25.0%
!! CTAAAATATGTGTTTTCTTG+CGG - Chr3:19502072-19502091 None:intergenic 25.0%
!! GATGTTTATGGCAATAATTT+TGG + Chr3:19502375-19502394 MsG0380012544.01.T01:CDS 25.0%
!! GCAAGAAAACACATATTTTA+GGG + Chr3:19502071-19502090 MsG0380012544.01.T01:CDS 25.0%
!! TAAAATATGTGTTTTCTTGC+GGG - Chr3:19502071-19502090 None:intergenic 25.0%
!! TTATGGCAATAATTTTGGAT+GGG + Chr3:19502380-19502399 MsG0380012544.01.T01:CDS 25.0%
!! TTTATGGCAATAATTTTGGA+TGG + Chr3:19502379-19502398 MsG0380012544.01.T01:CDS 25.0%
!! TTTTGTAGTTGCTTAATGAA+GGG - Chr3:19501832-19501851 None:intergenic 25.0%
!!! AATTCATTTTTTCCACTGAA+TGG + Chr3:19501571-19501590 MsG0380012544.01.T01:CDS 25.0%
!!! TATGGTCTATTATTTCTAGA+CGG - Chr3:19501429-19501448 None:intergenic 25.0%
AAAAATGAATTGAGAATGGG+TGG - Chr3:19501562-19501581 None:intergenic 30.0%
AAATGAAATAACCTCTCTGA+TGG - Chr3:19501922-19501941 None:intergenic 30.0%
AATGAAATAACCTCTCTGAT+GGG - Chr3:19501921-19501940 None:intergenic 30.0%
AATGGAATCAAAAACTACGA+AGG + Chr3:19501589-19501608 MsG0380012544.01.T01:CDS 30.0%
ATTGAAAGCCAATGTAGAAA+TGG + Chr3:19501969-19501988 MsG0380012544.01.T01:CDS 30.0%
CGCAGTGTAAATAATATAAG+TGG + Chr3:19502420-19502439 MsG0380012544.01.T01:CDS 30.0%
CTACAAAAGAACAAATCTGA+CGG + Chr3:19501844-19501863 MsG0380012544.01.T01:CDS 30.0%
GAAGAATGACTATGAGAATT+GGG + Chr3:19502269-19502288 MsG0380012544.01.T01:CDS 30.0%
TACAAAAGAACAAATCTGAC+GGG + Chr3:19501845-19501864 MsG0380012544.01.T01:CDS 30.0%
TGAAGAATGACTATGAGAAT+TGG + Chr3:19502268-19502287 MsG0380012544.01.T01:CDS 30.0%
TGTTACTATTACCAAGTCTA+AGG - Chr3:19502311-19502330 None:intergenic 30.0%
TTAATGAAGGGAAATCGTAT+GGG - Chr3:19501820-19501839 None:intergenic 30.0%
TTGAAAGCCAATGTAGAAAT+GGG + Chr3:19501970-19501989 MsG0380012544.01.T01:CDS 30.0%
TTGAGTGCAAAGAAATCATT+TGG - Chr3:19502239-19502258 None:intergenic 30.0%
! CGCAAGAAAACACATATTTT+AGG + Chr3:19502070-19502089 MsG0380012544.01.T01:CDS 30.0%
! CTTTTGTAGTTGCTTAATGA+AGG - Chr3:19501833-19501852 None:intergenic 30.0%
! GAGGTTATTTCATTTCACAA+AGG + Chr3:19501927-19501946 MsG0380012544.01.T01:CDS 30.0%
! TATGGCAATAATTTTGGATG+GGG + Chr3:19502381-19502400 MsG0380012544.01.T01:CDS 30.0%
! TTGAAGGGAAGATATTTTGT+TGG - Chr3:19501998-19502017 None:intergenic 30.0%
!! TAGTTTTTGATTCCATTCAG+TGG - Chr3:19501586-19501605 None:intergenic 30.0%
!! TATTTTGGAAATGCTGTTGT+AGG + Chr3:19502141-19502160 MsG0380012544.01.T01:CDS 30.0%
!! TGGTTTTACTTTTAACCATC+TGG + Chr3:19501669-19501688 MsG0380012544.01.T01:CDS 30.0%
AAATGCTGTTGTAGGTTCTA+TGG + Chr3:19502149-19502168 MsG0380012544.01.T01:CDS 35.0%
AACTCTCAACACTTAAACGT+TGG + Chr3:19501773-19501792 MsG0380012544.01.T01:CDS 35.0%
AATAGCCAACACACGATAAT+CGG - Chr3:19502337-19502356 None:intergenic 35.0%
AATCCAATCATCCTTAGACT+TGG + Chr3:19502297-19502316 MsG0380012544.01.T01:CDS 35.0%
ATTGAGAATGGGTGGAATAT+AGG - Chr3:19501554-19501573 None:intergenic 35.0%
CCAAAATAATCTTTCGGCAA+CGG - Chr3:19502129-19502148 None:intergenic 35.0%
CCTCTCTACAGATACAATAA+AGG + Chr3:19501231-19501250 MsG0380012544.01.T01:CDS 35.0%
CCTTTATTGTATCTGTAGAG+AGG - Chr3:19501234-19501253 None:intergenic 35.0%
CTATGGTTGCAATGAAAGTT+GGG + Chr3:19502166-19502185 MsG0380012544.01.T01:CDS 35.0%
CTTAATGAAGGGAAATCGTA+TGG - Chr3:19501821-19501840 None:intergenic 35.0%
TATGGTTGCAATGAAAGTTG+GGG + Chr3:19502167-19502186 MsG0380012544.01.T01:CDS 35.0%
TCTATGGTTGCAATGAAAGT+TGG + Chr3:19502165-19502184 MsG0380012544.01.T01:CDS 35.0%
TGGAATAGAAGAAGGTAGTA+TGG + Chr3:19502461-19502480 MsG0380012544.01.T01:CDS 35.0%
TTACCAAGTCTAAGGATGAT+TGG - Chr3:19502303-19502322 None:intergenic 35.0%
TTAGTTGACGGCATCTTTAT+TGG + Chr3:19501649-19501668 MsG0380012544.01.T01:CDS 35.0%
TTTAACCATCTGGTTGTTGA+TGG + Chr3:19501679-19501698 MsG0380012544.01.T01:CDS 35.0%
! GGGAAATGATCATGTTTTCA+TGG + Chr3:19502524-19502543 MsG0380012544.01.T01:CDS 35.0%
! GTATTTGCTGGAATAGAAGA+AGG + Chr3:19502453-19502472 MsG0380012544.01.T01:CDS 35.0%
!! ACGTTTAAGTGTTGAGAGTT+CGG - Chr3:19501773-19501792 None:intergenic 35.0%
!!! ACATATTTTAGGGTCTCGAT+AGG + Chr3:19502081-19502100 MsG0380012544.01.T01:CDS 35.0%
!!! AGTGTTTTAGCTGTCGAATT+TGG - Chr3:19501366-19501385 None:intergenic 35.0%
AAAAACTCAACCCATCAGAG+AGG + Chr3:19501908-19501927 MsG0380012544.01.T01:CDS 40.0%
AAATAATCTTTCGGCAACGG+TGG - Chr3:19502126-19502145 None:intergenic 40.0%
AAGCACTTTATGCACACCTT+TGG + Chr3:19502016-19502035 MsG0380012544.01.T01:CDS 40.0%
AAGTGTTCTCAATTGCAGCA+TGG - Chr3:19501507-19501526 None:intergenic 40.0%
AAGTTCCATCAACAACCAGA+TGG - Chr3:19501687-19501706 None:intergenic 40.0%
AATGAAAGTTGGGGAGTTGT+TGG + Chr3:19502176-19502195 MsG0380012544.01.T01:CDS 40.0%
ACTTGTGTAGGTGACATACT+TGG + Chr3:19501523-19501542 MsG0380012544.01.T01:CDS 40.0%
ATTGCCTCCAAAATCTTGTG+AGG - Chr3:19502504-19502523 None:intergenic 40.0%
CAAGTCACAGAGTTAGTTGA+CGG + Chr3:19501637-19501656 MsG0380012544.01.T01:CDS 40.0%
GCAATTGAGAACACTTGTGT+AGG + Chr3:19501511-19501530 MsG0380012544.01.T01:CDS 40.0%
GTTGGTACCCATTTCTACAT+TGG - Chr3:19501980-19501999 None:intergenic 40.0%
TCTCGATAGGAACTAGACAA+AGG + Chr3:19502094-19502113 MsG0380012544.01.T01:CDS 40.0%
TTGCCATAAACATCAAACCG+CGG - Chr3:19502369-19502388 None:intergenic 40.0%
TTGTATCTGTAGAGAGGACT+CGG - Chr3:19501228-19501247 None:intergenic 40.0%
! ACTCTAGAGGATGGTGATAA+AGG - Chr3:19501339-19501358 None:intergenic 40.0%
! CCGTTGCCGAAAGATTATTT+TGG + Chr3:19502126-19502145 MsG0380012544.01.T01:CDS 40.0%
! GTGATAAAGGAGACCCTTTT+GGG - Chr3:19501326-19501345 None:intergenic 40.0%
! GTGTCTTCCTCACAAGATTT+TGG + Chr3:19502494-19502513 MsG0380012544.01.T01:CDS 40.0%
! GTGTGCATAAAGTGCTTGAA+GGG - Chr3:19502013-19502032 None:intergenic 40.0%
! TCTGGTTGTTGATGGAACTT+CGG + Chr3:19501687-19501706 MsG0380012544.01.T01:CDS 40.0%
! TCTTCCTCACAAGATTTTGG+AGG + Chr3:19502497-19502516 MsG0380012544.01.T01:CDS 40.0%
! TGTTTTCTTGCGGGTCTAAT+TGG - Chr3:19502062-19502081 None:intergenic 40.0%
! TTGTTGATGGAACTTCGGTA+TGG + Chr3:19501692-19501711 MsG0380012544.01.T01:CDS 40.0%
!! ACGACATGGTGTTGTCTTTA+TGG - Chr3:19501447-19501466 None:intergenic 40.0%
!! CACAAGATTTTGGAGGCAAT+GGG + Chr3:19502504-19502523 MsG0380012544.01.T01:CDS 40.0%
!! CGAATTTGGTTTGACTCTAG+AGG - Chr3:19501352-19501371 None:intergenic 40.0%
!! CTTAAACGTTGGTTTCCAGA+TGG + Chr3:19501784-19501803 MsG0380012544.01.T01:CDS 40.0%
!! TCACAAGATTTTGGAGGCAA+TGG + Chr3:19502503-19502522 MsG0380012544.01.T01:CDS 40.0%
!! TTTGGTTTGACTCTAGAGGA+TGG - Chr3:19501348-19501367 None:intergenic 40.0%
ACCAGCATTGTTGCATGTGA+CGG - Chr3:19501473-19501492 None:intergenic 45.0%
AGTAGAACCTTGAGATCCCA+TGG - Chr3:19501292-19501311 None:intergenic 45.0%
ATGGGACGTTGAATACCATC+TGG - Chr3:19501802-19501821 None:intergenic 45.0%
CTTGTTGAACCCACCCAAAA+GGG + Chr3:19501310-19501329 MsG0380012544.01.T01:CDS 45.0%
TACGTCACAACCATTGCTTG+CGG + Chr3:19501612-19501631 MsG0380012544.01.T01:CDS 45.0%
TCTTACTCCATGGGATCTCA+AGG + Chr3:19501282-19501301 MsG0380012544.01.T01:CDS 45.0%
TGCCATAAACATCAAACCGC+GGG - Chr3:19502368-19502387 None:intergenic 45.0%
TGTGAGGAAGACACACTTGA+AGG - Chr3:19502488-19502507 None:intergenic 45.0%
TTCTTGCGGGTCTAATTGGT+CGG - Chr3:19502058-19502077 None:intergenic 45.0%
! ACTTGTTGAACCCACCCAAA+AGG + Chr3:19501309-19501328 MsG0380012544.01.T01:CDS 45.0%
! ATAAAGGAGACCCTTTTGGG+TGG - Chr3:19501323-19501342 None:intergenic 45.0%
! CATTGCCGATTATCGTGTGT+TGG + Chr3:19502329-19502348 MsG0380012544.01.T01:CDS 45.0%
! GGTGATAAAGGAGACCCTTT+TGG - Chr3:19501327-19501346 None:intergenic 45.0%
! GGTGTGCATAAAGTGCTTGA+AGG - Chr3:19502014-19502033 None:intergenic 45.0%
! TTTTGGATGGGGAAAACCTG+TGG + Chr3:19502392-19502411 MsG0380012544.01.T01:CDS 45.0%
!! GGACTGGTTGTTGTATTTGC+TGG + Chr3:19502441-19502460 MsG0380012544.01.T01:CDS 45.0%
!! TAAAGGAGACCCTTTTGGGT+GGG - Chr3:19501322-19501341 None:intergenic 45.0%
ATCTGTAGAGAGGACTCGGA+TGG - Chr3:19501224-19501243 None:intergenic 50.0%
GCCATAAACATCAAACCGCG+GGG - Chr3:19502367-19502386 None:intergenic 50.0%
GTTGGGGAGTTGTTGGAAGA+AGG + Chr3:19502183-19502202 MsG0380012544.01.T01:CDS 50.0%
TAATTGGTCGGAGCGGATCA+CGG - Chr3:19502046-19502065 None:intergenic 50.0%
TCCCCGCGGTTTGATGTTTA+TGG + Chr3:19502363-19502382 MsG0380012544.01.T01:CDS 50.0%
TCCGTCACATGCAACAATGC+TGG + Chr3:19501469-19501488 MsG0380012544.01.T01:CDS 50.0%
! ATTTCTAGACGGCCTGCAAG+TGG - Chr3:19501418-19501437 None:intergenic 50.0%
! TTGTTGGAAGAAGGCGCACT+TGG + Chr3:19502192-19502211 MsG0380012544.01.T01:CDS 50.0%
TGGATGGGGAAAACCTGTGG+CGG + Chr3:19502395-19502414 MsG0380012544.01.T01:CDS 55.0%
TGGCTATTAGCAGTTCCCCG+CGG + Chr3:19502349-19502368 MsG0380012544.01.T01:CDS 55.0%
TGGGCTGAAATCTCACGTGG+TGG + Chr3:19501730-19501749 MsG0380012544.01.T01:CDS 55.0%
TTTACACTGCGAACCGCCAC+AGG - Chr3:19502411-19502430 None:intergenic 55.0%
! GCATGTGACGGAACACGACA+TGG - Chr3:19501461-19501480 None:intergenic 55.0%
! GCCTGCAAGTGGTGGGAAAA+AGG - Chr3:19501407-19501426 None:intergenic 55.0%
! GCCTTTTTCCCACCACTTGC+AGG + Chr3:19501403-19501422 MsG0380012544.01.T01:CDS 55.0%
! GTGACTTGCACCGCAAGCAA+TGG - Chr3:19501625-19501644 None:intergenic 55.0%
! TCGTGGGCTGAAATCTCACG+TGG + Chr3:19501727-19501746 MsG0380012544.01.T01:CDS 55.0%
CTAGACGGCCTGCAAGTGGT+GGG - Chr3:19501414-19501433 None:intergenic 60.0%
GCGGGTCTAATTGGTCGGAG+CGG - Chr3:19502053-19502072 None:intergenic 60.0%
TCTAGACGGCCTGCAAGTGG+TGG - Chr3:19501415-19501434 None:intergenic 60.0%
! AGCGGATCACGGAGCACCAA+AGG - Chr3:19502035-19502054 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr3 gene 19501214 19502563 19501214 ID=MsG0380012544.01;Name=MsG0380012544.01
Chr3 mRNA 19501214 19502563 19501214 ID=MsG0380012544.01.T01;Parent=MsG0380012544.01;Name=MsG0380012544.01.T01;_AED=0.20;_eAED=0.20;_QI=0|-1|0|1|-1|1|1|0|449
Chr3 exon 19501214 19502563 19501214 ID=MsG0380012544.01.T01:exon:3862;Parent=MsG0380012544.01.T01
Chr3 CDS 19501214 19502563 19501214 ID=MsG0380012544.01.T01:cds;Parent=MsG0380012544.01.T01
Gene Sequence

>MsG0380012544.01.T01

ATGGCTTCCATCCGAGTCCTCTCTACAGATACAATAAAGGCACCAAATTCAACTGATGAAAAAATTGATCTTACTCCATGGGATCTCAAGGTTCTACTTGTTGAACCCACCCAAAAGGGTCTCCTTTATCACCATCCTCTAGAGTCAAACCAAATTCGACAGCTAAAACACTCTCTATCTTCTGCTCTTGCCTTTTTCCCACCACTTGCAGGCCGTCTAGAAATAATAGACCATAAAGACAACACCATGTCGTGTTCCGTCACATGCAACAATGCTGGTGTACTCTTTGTCCATGCTGCAATTGAGAACACTTGTGTAGGTGACATACTTGGATGCACCTATATTCCACCCATTCTCAATTCATTTTTTCCACTGAATGGAATCAAAAACTACGAAGGTACGTCACAACCATTGCTTGCGGTGCAAGTCACAGAGTTAGTTGACGGCATCTTTATTGGTTTTACTTTTAACCATCTGGTTGTTGATGGAACTTCGGTATGGTATTTCATTAATTCGTGGGCTGAAATCTCACGTGGTGGTTGTTGCAATCAAATATCCGAACTCTCAACACTTAAACGTTGGTTTCCAGATGGTATTCAACGTCCCATACGATTTCCCTTCATTAAGCAACTACAAAAGAACAAATCTGACGGGTTGAGTTTTTCATCATCTAATAACGAAAAACTCAATAACGAAAAACTCAACCCATCAGAGAGGTTATTTCATTTCACAAAGGAGAAAATCTCTCAATTGAAATTGAAAGCCAATGTAGAAATGGGTACCAACAAAATATCTTCCCTTCAAGCACTTTATGCACACCTTTGGTGCTCCGTGATCCGCTCCGACCAATTAGACCCGCAAGAAAACACATATTTTAGGGTCTCGATAGGAACTAGACAAAGGTTTGTTCCACCGTTGCCGAAAGATTATTTTGGAAATGCTGTTGTAGGTTCTATGGTTGCAATGAAAGTTGGGGAGTTGTTGGAAGAAGGCGCACTTGGTAAATGTGCTTCGAAGATGCACCAAATGATTTCTTTGCACTCAAATGAGAAGTTGAAGAATGACTATGAGAATTGGGTGAGAAATCCAATCATCCTTAGACTTGGTAATAGTAACATTGCCGATTATCGTGTGTTGGCTATTAGCAGTTCCCCGCGGTTTGATGTTTATGGCAATAATTTTGGATGGGGAAAACCTGTGGCGGTTCGCAGTGTAAATAATATAAGTGGACTGGTTGTTGTATTTGCTGGAATAGAAGAAGGTAGTATGGACCTTCAAGTGTGTCTTCCTCACAAGATTTTGGAGGCAATGGGAAATGATCATGTTTTCATGGACTTTGTATCGAATTAA
Protein sequence

>MsG0380012544.01.T01

MASIRVLSTDTIKAPNSTDEKIDLTPWDLKVLLVEPTQKGLLYHHPLESNQIRQLKHSLSSALAFFPPLAGRLEIIDHKDNTMSCSVTCNNAGVLFVHAAIENTCVGDILGCTYIPPILNSFFPLNGIKNYEGTSQPLLAVQVTELVDGIFIGFTFNHLVVDGTSVWYFINSWAEISRGGCCNQISELSTLKRWFPDGIQRPIRFPFIKQLQKNKSDGLSFSSSNNEKLNNEKLNPSERLFHFTKEKISQLKLKANVEMGTNKISSLQALYAHLWCSVIRSDQLDPQENTYFRVSIGTRQRFVPPLPKDYFGNAVVGSMVAMKVGELLEEGALGKCASKMHQMISLHSNEKLKNDYENWVRNPIILRLGNSNIADYRVLAISSSPRFDVYGNNFGWGKPVAVRSVNNISGLVVVFAGIEEGSMDLQVCLPHKILEAMGNDHVFMDFVSN*