AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280006456.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006456.01.T01 MTR_2g101460 76.316 190 37 1 1 190 20 201 7.45e-91 267
MsG0280006456.01.T01 MTR_2g101460 76.316 190 37 1 1 190 10 191 2.06e-90 265
MsG0280006456.01.T01 MTR_3g448650 97.321 112 3 0 1 112 47 158 5.64e-76 228
MsG0280006456.01.T01 MTR_1g070355 45.622 217 118 0 18 234 56 272 1.89e-62 205
MsG0280006456.01.T01 MTR_6g022080 42.391 184 106 0 16 199 65 248 3.72e-50 165
MsG0280006456.01.T01 MTR_5g076070 42.268 194 112 0 41 234 58 251 3.60e-48 160
MsG0280006456.01.T01 MTR_1g052260 59.615 104 42 0 15 118 55 158 1.26e-40 137
MsG0280006456.01.T01 MTR_6g053140 42.308 130 67 2 17 145 84 206 2.21e-27 105
MsG0280006456.01.T01 MTR_8g066350 37.589 141 66 2 8 144 47 169 2.95e-25 98.2
MsG0280006456.01.T01 MTR_7g446520 30.732 205 122 3 27 229 79 265 2.77e-22 94.0
MsG0280006456.01.T01 MTR_0060s0090 42.683 82 47 0 39 120 59 140 9.83e-20 83.2
MsG0280006456.01.T01 MTR_7g028855 31.507 146 100 0 32 177 116 261 6.82e-18 82.8
MsG0280006456.01.T01 MTR_3g450850 31.088 193 117 6 48 227 59 248 4.81e-16 76.6
MsG0280006456.01.T01 MTR_0402s0040 25.837 209 145 5 38 239 28 233 4.10e-15 74.7
MsG0280006456.01.T01 MTR_4g050510 29.921 127 75 1 46 172 88 200 6.18e-15 71.6
MsG0280006456.01.T01 MTR_0604s0020 45.333 75 41 0 46 120 2 76 8.84e-15 67.8
MsG0280006456.01.T01 MTR_6g463250 37.143 105 51 2 16 120 68 157 1.34e-14 70.1
MsG0280006456.01.T01 MTR_1g008290 30.952 126 83 3 93 215 2 126 7.29e-12 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006456.01.T01 AT5G28530 30.864 162 108 3 48 206 67 227 1.50e-18 85.1
MsG0280006456.01.T01 AT5G28530 30.864 162 108 3 48 206 67 227 1.64e-18 84.7
MsG0280006456.01.T01 AT5G28530 30.864 162 108 3 48 206 67 227 1.64e-18 84.7
MsG0280006456.01.T01 AT5G28530 30.864 162 108 3 48 206 67 227 1.68e-18 84.7
MsG0280006456.01.T01 AT5G28530 30.864 162 108 3 48 206 67 227 1.92e-18 84.7
MsG0280006456.01.T01 AT4G38180 33.654 208 126 7 28 229 67 268 1.03e-16 79.7
MsG0280006456.01.T01 AT1G10240 32.576 132 84 3 47 174 59 189 8.90e-14 70.9
MsG0280006456.01.T01 AT1G10240 32.576 132 84 3 47 174 59 189 8.90e-14 70.9

Find 29 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTATAAGCCACTGCAATATT+TGG 0.202241 2:-2182924 None:intergenic
TTGCATCACTTTATGGAGTT+TGG 0.282438 2:+2182786 None:intergenic
ATTGAAGGAATGGAAGATAT+AGG 0.332722 2:+2182849 MsG0280006456.01.T01:CDS
ATTAGAACTTCACAAGCTTA+TGG 0.352387 2:+2183305 MsG0280006456.01.T01:CDS
AGTGATGTGAAAGTTGGATT+TGG 0.354794 2:+2182819 MsG0280006456.01.T01:CDS
GTAGTCAACGTTGGTATGTA+TGG 0.377945 2:+2183162 MsG0280006456.01.T01:CDS
ATCAATGTGCACACTACATT+TGG 0.382053 2:-2183135 None:intergenic
TTGGATTTCTTCCGGGTCAC+AGG 0.383991 2:+2183231 MsG0280006456.01.T01:CDS
TGTAGTGTGCACATTGATTC+TGG 0.404970 2:+2183140 MsG0280006456.01.T01:CDS
AATTGGTATGCTCGAATGAA+TGG 0.408021 2:+2182954 MsG0280006456.01.T01:CDS
TTGAAGGAATGGAAGATATA+GGG 0.408538 2:+2182850 MsG0280006456.01.T01:CDS
TAGTGATTCTAGAGCTGCTT+TGG 0.425882 2:+2183421 MsG0280006456.01.T01:CDS
ATAGAAAGTGATGTGAAAGT+TGG 0.433335 2:+2182813 MsG0280006456.01.T01:CDS
CATGTACAATGAAGTTGATA+AGG 0.435376 2:+2183382 MsG0280006456.01.T01:CDS
GGATTTGGATTTGCTATTGA+AGG 0.453071 2:+2182834 MsG0280006456.01.T01:CDS
TGGATTTCTTCCGGGTCACA+GGG 0.456241 2:+2183232 MsG0280006456.01.T01:CDS
GATAAGGATAGACGAAGTAA+AGG 0.470352 2:+2183398 MsG0280006456.01.T01:CDS
CTGCTCGTAGAAGCAAAGTA+AGG 0.491172 2:+2182982 MsG0280006456.01.T01:CDS
GATTTCTTCCGGGTCACAGG+GGG 0.500838 2:+2183234 MsG0280006456.01.T01:CDS
TGGATTTGCTATTGAAGGAA+TGG 0.513659 2:+2182839 MsG0280006456.01.T01:CDS
ATTTCTTCCGGGTCACAGGG+GGG 0.543862 2:+2183235 MsG0280006456.01.T01:CDS
TAGTCAACGTTGGTATGTAT+GGG 0.562663 2:+2183163 MsG0280006456.01.T01:CDS
CAATAAAATCATCATCGACA+AGG 0.586633 2:-2183211 None:intergenic
GTGAGGCAAGAGCTGACACT+AGG 0.593063 2:+2183099 MsG0280006456.01.T01:CDS
CATCGACAAGGCTATGATTG+TGG 0.600537 2:-2183199 None:intergenic
TTGATTCTGGTAGTCAACGT+TGG 0.631816 2:+2183153 MsG0280006456.01.T01:CDS
ATATTTGTTTGCTACAGACA+AGG 0.641722 2:+2183035 MsG0280006456.01.T01:CDS
CAATAAAATCTGTAAGCGTG+AGG 0.648827 2:+2183082 MsG0280006456.01.T01:CDS
GGATTTCTTCCGGGTCACAG+GGG 0.653981 2:+2183233 MsG0280006456.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACTTTCTAAGATTTTTCATT+TGG - Chr2:2183280-2183299 None:intergenic 20.0%
! ATGAAAAATCTTAGAAAGTC+TGG + Chr2:2183281-2183300 MsG0280006456.01.T01:CDS 25.0%
!! TGATTTTATTGGATTTCTTC+CGG + Chr2:2183223-2183242 MsG0280006456.01.T01:CDS 25.0%
!! TGGCTTATACATTTTACAAT+TGG + Chr2:2182937-2182956 MsG0280006456.01.T01:CDS 25.0%
ATAGAAAGTGATGTGAAAGT+TGG + Chr2:2182813-2182832 MsG0280006456.01.T01:CDS 30.0%
ATTAGAACTTCACAAGCTTA+TGG + Chr2:2183305-2183324 MsG0280006456.01.T01:CDS 30.0%
ATTGAAGGAATGGAAGATAT+AGG + Chr2:2182849-2182868 MsG0280006456.01.T01:CDS 30.0%
CAATAAAATCATCATCGACA+AGG - Chr2:2183214-2183233 None:intergenic 30.0%
TTGAAGGAATGGAAGATATA+GGG + Chr2:2182850-2182869 MsG0280006456.01.T01:CDS 30.0%
! ATATTTGTTTGCTACAGACA+AGG + Chr2:2183035-2183054 MsG0280006456.01.T01:CDS 30.0%
! CTTGTCGATGATGATTTTAT+TGG + Chr2:2183212-2183231 MsG0280006456.01.T01:CDS 30.0%
! GATTTTATTGGATTTCTTCC+GGG + Chr2:2183224-2183243 MsG0280006456.01.T01:CDS 30.0%
! TCATTTTCCAAATATTGCAG+TGG + Chr2:2182917-2182936 MsG0280006456.01.T01:CDS 30.0%
!! CATGTACAATGAAGTTGATA+AGG + Chr2:2183382-2183401 MsG0280006456.01.T01:CDS 30.0%
AATTGGTATGCTCGAATGAA+TGG + Chr2:2182954-2182973 MsG0280006456.01.T01:CDS 35.0%
AGTGATGTGAAAGTTGGATT+TGG + Chr2:2182819-2182838 MsG0280006456.01.T01:CDS 35.0%
ATCAATGTGCACACTACATT+TGG - Chr2:2183138-2183157 None:intergenic 35.0%
CAATAAAATCTGTAAGCGTG+AGG + Chr2:2183082-2183101 MsG0280006456.01.T01:CDS 35.0%
GATAAGGATAGACGAAGTAA+AGG + Chr2:2183398-2183417 MsG0280006456.01.T01:CDS 35.0%
GTATAAGCCACTGCAATATT+TGG - Chr2:2182927-2182946 None:intergenic 35.0%
GTATTCCTCAAAAACATTGC+TGG - Chr2:2183476-2183495 None:intergenic 35.0%
TAGTCAACGTTGGTATGTAT+GGG + Chr2:2183163-2183182 MsG0280006456.01.T01:CDS 35.0%
!! ATGATCCAGCAATGTTTTTG+AGG + Chr2:2183468-2183487 MsG0280006456.01.T01:CDS 35.0%
!! GGATTTGGATTTGCTATTGA+AGG + Chr2:2182834-2182853 MsG0280006456.01.T01:CDS 35.0%
!! TGGATTTGCTATTGAAGGAA+TGG + Chr2:2182839-2182858 MsG0280006456.01.T01:CDS 35.0%
GTAGTCAACGTTGGTATGTA+TGG + Chr2:2183162-2183181 MsG0280006456.01.T01:CDS 40.0%
TTGATTCTGGTAGTCAACGT+TGG + Chr2:2183153-2183172 MsG0280006456.01.T01:CDS 40.0%
! ACTTTTGCTAATCACTACGG+GGG + Chr2:2183329-2183348 MsG0280006456.01.T01:CDS 40.0%
! CACTTTTGCTAATCACTACG+GGG + Chr2:2183328-2183347 MsG0280006456.01.T01:CDS 40.0%
! GCACTTTTGCTAATCACTAC+GGG + Chr2:2183327-2183346 MsG0280006456.01.T01:CDS 40.0%
! GGCACTTTTGCTAATCACTA+CGG + Chr2:2183326-2183345 MsG0280006456.01.T01:CDS 40.0%
!! TAGTGATTCTAGAGCTGCTT+TGG + Chr2:2183421-2183440 MsG0280006456.01.T01:CDS 40.0%
!! TGTAGTGTGCACATTGATTC+TGG + Chr2:2183140-2183159 MsG0280006456.01.T01:CDS 40.0%
!!! ACAGTTGTCGCGTCATTTTT+CGG + Chr2:2183494-2183513 MsG0280006456.01.T01:CDS 40.0%
CATCGACAAGGCTATGATTG+TGG - Chr2:2183202-2183221 None:intergenic 45.0%
CTGCTCGTAGAAGCAAAGTA+AGG + Chr2:2182982-2183001 MsG0280006456.01.T01:CDS 45.0%
!!! GCGTCATTTTTCGGAGATCA+AGG + Chr2:2183503-2183522 MsG0280006456.01.T01:CDS 45.0%
TGGATTTCTTCCGGGTCACA+GGG + Chr2:2183232-2183251 MsG0280006456.01.T01:CDS 50.0%
TTGGATTTCTTCCGGGTCAC+AGG + Chr2:2183231-2183250 MsG0280006456.01.T01:CDS 50.0%
ATTTCTTCCGGGTCACAGGG+GGG + Chr2:2183235-2183254 MsG0280006456.01.T01:CDS 55.0%
GATTTCTTCCGGGTCACAGG+GGG + Chr2:2183234-2183253 MsG0280006456.01.T01:CDS 55.0%
GGATTTCTTCCGGGTCACAG+GGG + Chr2:2183233-2183252 MsG0280006456.01.T01:CDS 55.0%
GTGAGGCAAGAGCTGACACT+AGG + Chr2:2183099-2183118 MsG0280006456.01.T01:CDS 55.0%
TCATCCACCCCCCTGTGACC+CGG - Chr2:2183245-2183264 None:intergenic 65.0%
TCTTCCGGGTCACAGGGGGG+TGG + Chr2:2183238-2183257 MsG0280006456.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr2 gene 2182798 2183544 2182798 ID=MsG0280006456.01;Name=MsG0280006456.01
Chr2 mRNA 2182798 2183544 2182798 ID=MsG0280006456.01.T01;Parent=MsG0280006456.01;Name=MsG0280006456.01.T01;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|1|1|0|248
Chr2 exon 2182798 2183544 2182798 ID=MsG0280006456.01.T01:exon:327;Parent=MsG0280006456.01.T01
Chr2 CDS 2182798 2183544 2182798 ID=MsG0280006456.01.T01:cds;Parent=MsG0280006456.01.T01
Gene Sequence

>MsG0280006456.01.T01

ATGGAGTTTGGAGATATAGAAAGTGATGTGAAAGTTGGATTTGGATTTGCTATTGAAGGAATGGAAGATATAGGGAAAATTGATTTTAAAAAATTAAATGCAAGTGATGTTATGAAGTTTCATTTTCCAAATATTGCAGTGGCTTATACATTTTACAATTGGTATGCTCGAATGAATGGTTTTTCTGCTCGTAGAAGCAAAGTAAGGAGAAACAAAAACAACGAGATTATACAACAAATATTTGTTTGCTACAGACAAGGTTTCAGAGAAAAAAAGCTTGAGAACAATAAAATCTGTAAGCGTGAGGCAAGAGCTGACACTAGGTGCAGATGTGATGCCAAATGTAGTGTGCACATTGATTCTGGTAGTCAACGTTGGTATGTATGGGATTTTAACAATGACCACAATCATAGCCTTGTCGATGATGATTTTATTGGATTTCTTCCGGGTCACAGGGGGGTGGATGATGATGACATTCTCCAAATGAAAAATCTTAGAAAGTCTGGTATTAGAACTTCACAAGCTTATGGCACTTTTGCTAATCACTACGGGGGATACAAAAATGTGACATTTTTGATACAAGACATGTACAATGAAGTTGATAAGGATAGACGAAGTAAAGGTAGTGATTCTAGAGCTGCTTTGGCATTTTTACACTCTCTGAAGATTGATGATCCAGCAATGTTTTTGAGGAATACAGTTGTCGCGTCATTTTTCGGAGATCAAGGCTATTTGATTAGCTTTTGA

Protein sequence

>MsG0280006456.01.T01

MEFGDIESDVKVGFGFAIEGMEDIGKIDFKKLNASDVMKFHFPNIAVAYTFYNWYARMNGFSARRSKVRRNKNNEIIQQIFVCYRQGFREKKLENNKICKREARADTRCRCDAKCSVHIDSGSQRWYVWDFNNDHNHSLVDDDFIGFLPGHRGVDDDDILQMKNLRKSGIRTSQAYGTFANHYGGYKNVTFLIQDMYNEVDKDRRSKGSDSRAALAFLHSLKIDDPAMFLRNTVVASFFGDQGYLISF*