AlfalfaGEDB | MsG0680031852.01

M. sativa cultivar ZhongmuNo.1 / MsG0680031852.01

KEGG: -
GO: GO:0016021 GO:0055085
PFAM: PF03595

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680031852.01.T01	XP_003619247.3	90.083	605	22	2	11	578	1	604	0	1090

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680031852.01.T01	Q9FLV9	53.016	630	180	12	27	562	14	621	0	617

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680031852.01.T01	A0A1C3RMF1	89.752	605	25	1	11	578	1	605	0.0	1090

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048506.01	MsG0680031852.01	0.845792	3.255580e-59	5.488543e-56
MsG0080048507.01	MsG0680031852.01	0.854798	9.678992e-62	2.187806e-58
MsG0180001029.01	MsG0680031852.01	0.815383	9.508453e-52	6.545786e-49
MsG0180003404.01	MsG0680031852.01	0.816555	5.206097e-52	3.700668e-49
MsG0180003992.01	MsG0680031852.01	0.813765	2.168402e-51	1.428632e-48
MsG0180004880.01	MsG0680031852.01	0.823556	1.299815e-53	1.122887e-50
MsG0180005906.01	MsG0680031852.01	0.803398	3.553979e-49	1.784119e-46
MsG0180006195.01	MsG0680031852.01	0.802685	4.989375e-49	2.459454e-46
MsG0280007853.01	MsG0680031852.01	-0.803233	3.843279e-49	1.921166e-46
MsG0280011110.01	MsG0680031852.01	0.829114	6.168034e-55	6.252989e-52

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680031852.01.T01	MTR_6g045200	89.917	605	23	2	11	578	1	604	0.0	1089
MsG0680031852.01.T01	MTR_6g034030	83.089	615	54	5	11	577	1	613	0.0	1013
MsG0680031852.01.T01	MTR_0549s0020	89.885	524	16	1	74	560	1	524	0.0	954
MsG0680031852.01.T01	MTR_6g034030	80.544	478	43	5	11	440	1	476	0.0	754
MsG0680031852.01.T01	MTR_4g101880	46.125	542	271	6	40	575	30	556	4.23e-168	489
MsG0680031852.01.T01	MTR_0549s0010	73.077	208	18	2	74	244	1	207	2.46e-92	282
MsG0680031852.01.T01	MTR_4g049640	43.408	311	165	4	206	506	53	362	9.31e-67	221
MsG0680031852.01.T01	MTR_3g112530	41.411	326	178	6	206	521	51	373	4.03e-63	212
MsG0680031852.01.T01	MTR_4g132400	35.077	325	201	5	191	505	32	356	5.79e-56	193

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680031852.01.T01	AT5G24030	53.016	630	180	12	27	562	14	621	0.0	617
MsG0680031852.01.T01	AT4G27970	59.188	468	168	5	118	565	41	505	0.0	557
MsG0680031852.01.T01	AT4G27970	59.188	468	168	5	118	565	41	505	0.0	557
MsG0680031852.01.T01	AT5G24030	52.427	515	133	10	27	450	14	507	1.65e-168	489
MsG0680031852.01.T01	AT4G27970	64.305	367	127	3	208	573	1	364	1.94e-165	476
MsG0680031852.01.T01	AT4G27970	64.305	367	127	3	208	573	1	364	1.94e-165	476
MsG0680031852.01.T01	AT1G12480	58.228	395	158	2	145	532	132	526	5.26e-161	471
MsG0680031852.01.T01	AT4G27970	62.682	343	109	2	118	441	41	383	1.50e-151	441
MsG0680031852.01.T01	AT4G27970	62.682	343	109	2	118	441	41	383	1.50e-151	441
MsG0680031852.01.T01	AT1G62280	39.542	306	176	6	206	503	46	350	7.37e-69	228

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Find 115 sgRNAs with CRISPR-Local

Find 270 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACCTACATCAATCTCTA+TGG	0.381498	6:+28207947	MsG0680031852.01.T01:CDS
AACAATGGCCGTTTCTTCTC+CGG	0.397146	6:+28207626	MsG0680031852.01.T01:CDS
AACACTGGATGTAGCTCTTT+AGG	0.372096	6:-28208225	None:intergenic
AACCCTAACCAGTGTTTATG+TGG	0.454199	6:-28208994	None:intergenic
AACGGCCATTGTTTGTCATG+AGG	0.606151	6:-28207616	None:intergenic
AACTTACCAATGAGAAATAA+AGG	0.395497	6:-28208350	None:intergenic
AACTTTGTTGGAGCGTTACT+TGG	0.463926	6:+28208090	MsG0680031852.01.T01:CDS
AAGAAGAAGTTAACACGGAT+TGG	0.491885	6:-28207868	None:intergenic
AAGCCACATAAACACTGGTT+AGG	0.404899	6:+28208991	MsG0680031852.01.T01:CDS
AAGTCTCAACCAATGCCAAA+AGG	0.547948	6:+28207305	MsG0680031852.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAACCTACATCAATCTCTA+TGG	+	Chr6:28207947-28207966	MsG0680031852.01.T01:CDS	30.0%
ATCTCTATGGTACATTCTTA+TGG	+	Chr6:28207960-28207979	MsG0680031852.01.T01:CDS	30.0%
ATGTGATTCTTGTTGAAGTT+TGG	+	Chr6:28205430-28205449	MsG0680031852.01.T01:intron	30.0%
CTTAAAAAGGAGTCTTACTT+TGG	-	Chr6:28206601-28206620	None:intergenic	30.0%
GAATTCTCATGAAGATATGA+AGG	-	Chr6:28204980-28204999	None:intergenic	30.0%
GACTTAAGAGTTTCTAATTC+TGG	-	Chr6:28207577-28207596	None:intergenic	30.0%
GAGACTGTTTGTATTACTTA+CGG	+	Chr6:28206694-28206713	MsG0680031852.01.T01:intron	30.0%
GTTGAGTTACAATAGAATCA+AGG	-	Chr6:28206412-28206431	None:intergenic	30.0%
TATATCTGGTCCTAAGTTAT+AGG	-	Chr6:28205850-28205869	None:intergenic	30.0%
TCCAAATCATGAACATAAAG+AGG	-	Chr6:28206676-28206695	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr6	gene	28204853	28209146	28204853	ID=MsG0680031852.01;Name=MsG0680031852.01
Chr6	mRNA	28204853	28209146	28204853	ID=MsG0680031852.01.T01;Parent=MsG0680031852.01;Name=MsG0680031852.01.T01;_AED=0.35;_eAED=0.36;_QI=0\|0\|0\|1\|1\|1\|6\|0\|578
Chr6	exon	28204853	28204872	28204853	ID=MsG0680031852.01.T01:exon:8807;Parent=MsG0680031852.01.T01
Chr6	exon	28206455	28206602	28206455	ID=MsG0680031852.01.T01:exon:8808;Parent=MsG0680031852.01.T01
Chr6	exon	28207107	28207371	28207107	ID=MsG0680031852.01.T01:exon:8809;Parent=MsG0680031852.01.T01
Chr6	exon	28207482	28208365	28207482	ID=MsG0680031852.01.T01:exon:8810;Parent=MsG0680031852.01.T01
Chr6	exon	28208623	28208654	28208623	ID=MsG0680031852.01.T01:exon:8811;Parent=MsG0680031852.01.T01
Chr6	exon	28208759	28209146	28208759	ID=MsG0680031852.01.T01:exon:8812;Parent=MsG0680031852.01.T01
Chr6	CDS	28204853	28204872	28204853	ID=MsG0680031852.01.T01:cds;Parent=MsG0680031852.01.T01
Chr6	CDS	28206455	28206602	28206455	ID=MsG0680031852.01.T01:cds;Parent=MsG0680031852.01.T01
Chr6	CDS	28207107	28207371	28207107	ID=MsG0680031852.01.T01:cds;Parent=MsG0680031852.01.T01
Chr6	CDS	28207482	28208365	28207482	ID=MsG0680031852.01.T01:cds;Parent=MsG0680031852.01.T01
Chr6	CDS	28208623	28208654	28208623	ID=MsG0680031852.01.T01:cds;Parent=MsG0680031852.01.T01
Chr6	CDS	28208759	28209146	28208759	ID=MsG0680031852.01.T01:cds;Parent=MsG0680031852.01.T01

Gene Sequence

>MsG0680031852.01.T01

ATGTTGAAGTCGGCAAATAGGTTTAGCAGCATGGAAAATCACATAACTCTTGAAATCACAGAACAAGCTTCTCCTCCAGAAACTCCATCACTAATAAAGTATATATCATCAAATGAACTTGAAGATTTTGATTCATTTGAATCTCAGTTTCCAAGCCCAATTTCCAAAGAAAGTGAAGAAACATATAATCATCAAAGAAAACCTTCAATGTCTGTCAGCATGCCACTTTGTTATAAAGAAACTCAACTTCAATCACTAAACATCAACAATACCAAGAATGTTTCCTTTAGTGGAGAAAATGTTATTATCAAAGATGATTTTCCATCAGGAATTGCAATGTCTGAGCCTCCAAGACAACCAAAATTCAAGTCTCAACCAATGCCAAAAGGAGTTACTTACCAACAAGATGGTTCTCAAACTCGAAAGGCTAATCAACCGAGTGGAAATGCACAAGATGGTAACAACGCTACAAGAAGCTTTGATAGGGCATTACCTGTTGATAGGTATTTTGATGCATTGGAAGGTCCAGAATTAGAAACTCTTAAGTCATCAGAAGAGATAATGCTACCTCATGACAAACAATGGCCGTTTCTTCTCCGGTTCCCCGTTTCATCGTTCGGTATATGCCTCGGAGTTAGCAGCCAAGCAATTCTTTGGAAAACCCTAGCCACATCGCCTACCACCGAATTCCTTCACATAACTCCCAAAATAAACCTAATCTTATGGTACATCTCCGCGGTACTTATAGCTACCATTTTCGCTGTCTATATTCTCAAACTCCTTTTCTACTTCGAAGCAGTTCGTCGGGAATACTACCATCCAATCCGTGTTAACTTCTTCTTCGCACCGTGGATAGCTCTCTTGTTTTTAGCACTAGGAGTTCCTCCATCCGTAACCAAAAACCTACATCAATCTCTATGGTACATTCTTATGGCACCAATATTATGTCTCGAGCTTAAGATTTACGGACAATGGATGTCCGGTGGACAAAGGCGGCTTTCGAAGGTGGCGAACCCTTCAAATCATTTATCAGTTGTTGGAAACTTTGTTGGAGCGTTACTTGGTGCATCGATGGGACTAATTGAAGGACCAATTTTCTTCTTTGCTGTTGGACTTGCTCATTACACTGTGTTATTTGTGACACTTTATCAAAGACTTCCAACCAATGCAACACTACCTAAAGAGCTACATCCAGTGTTCTTTCTCTTTGTTGCAGCTCCTAGTGTTGCTTCAATGGCTTGGGCTAAGATTCAAGGTTCTTTTGATTATGGATCAAGGATTGCTTATTTCATTGCATTGTTCCTTTATTTCTCATTGGCTGTCAGGATCAATTTCTTCAGAGGATTTAAATTTTCTCTTGCATGGTGGGCCTATACTTTTCCAATGACTGGTGCAGCAATTGCAACAATAAGATACTCAAATCAAGTACCAAATATTGTCACTAAGTCATTGTGTGTTGCATTGGCTCTAATCTCAACATTCACGGTTATGGCACTTCTTCTATCAACAATATTGCATGCATTTGTCTTTAGAGATCTCTTCCCTAATGACATTGCTATTGCCATTAGTGATAGAAGGAGAAAGCCACATAAACACTGGTTAGGGTTCAGATATGGAAGCCAAGACTCCAAAGAGATTGAAAATTACTTGAAGTTTGTGAACACTGATGATAGTAGTTTGGATGATTCTACGCCACAACCGTCCTCAAACGATGCCGATCAGAATTTGCCAAAATAA

Protein sequence

>MsG0680031852.01.T01

MLKSANRFSSMENHITLEITEQASPPETPSLIKYISSNELEDFDSFESQFPSPISKESEETYNHQRKPSMSVSMPLCYKETQLQSLNINNTKNVSFSGENVIIKDDFPSGIAMSEPPRQPKFKSQPMPKGVTYQQDGSQTRKANQPSGNAQDGNNATRSFDRALPVDRYFDALEGPELETLKSSEEIMLPHDKQWPFLLRFPVSSFGICLGVSSQAILWKTLATSPTTEFLHITPKINLILWYISAVLIATIFAVYILKLLFYFEAVRREYYHPIRVNFFFAPWIALLFLALGVPPSVTKNLHQSLWYILMAPILCLELKIYGQWMSGGQRRLSKVANPSNHLSVVGNFVGALLGASMGLIEGPIFFFAVGLAHYTVLFVTLYQRLPTNATLPKELHPVFFLFVAAPSVASMAWAKIQGSFDYGSRIAYFIALFLYFSLAVRINFFRGFKFSLAWWAYTFPMTGAAIATIRYSNQVPNIVTKSLCVALALISTFTVMALLLSTILHAFVFRDLFPNDIAIAISDRRRKPHKHWLGFRYGSQDSKEIENYLKFVNTDDSSLDDSTPQPSSNDADQNLPK*