Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032521.01.T01 | XP_013453052.1 | 99.474 | 190 | 1 | 0 | 1 | 190 | 450 | 639 | 1.95E-130 | 390 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032521.01.T01 | Q9LHF5 | 43.814 | 194 | 70 | 6 | 1 | 181 | 430 | 597 | 8.51E-37 | 137 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032521.01.T01 | A0A072UMI5 | 99.474 | 190 | 1 | 0 | 1 | 190 | 450 | 639 | 9.33e-131 | 390 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048784.01 | MsG0680032521.01 | 0.832942 | 7.087873e-56 | 8.042198e-53 |
| MsG0180000806.01 | MsG0680032521.01 | 0.802127 | 6.502087e-49 | 3.159812e-46 |
| MsG0180002547.01 | MsG0680032521.01 | 0.808593 | 2.870211e-50 | 1.647712e-47 |
| MsG0380013183.01 | MsG0680032521.01 | 0.817203 | 3.724631e-52 | 2.695055e-49 |
| MsG0680032521.01 | MsG0780041258.01 | 0.810504 | 1.115548e-50 | 6.736098e-48 |
| MsG0680032521.01 | MsG0780041435.01 | 0.830020 | 3.715015e-55 | 3.866894e-52 |
| MsG0680032521.01 | MsG0880042324.01 | 0.822474 | 2.323475e-53 | 1.946815e-50 |
| MsG0680032521.01 | MsG0880042519.01 | 0.811940 | 5.443444e-51 | 3.414596e-48 |
| MsG0680032521.01 | MsG0880043081.01 | 0.805233 | 1.473799e-49 | 7.756160e-47 |
| MsG0680032521.01 | MsG0880044565.01 | 0.801016 | 1.098530e-48 | 5.190947e-46 |
| MsG0480021870.01 | MsG0680032521.01 | 0.800206 | 1.606744e-48 | 7.437461e-46 |
| MsG0580025439.01 | MsG0680032521.01 | 0.813294 | 2.752797e-51 | 1.790642e-48 |
| MsG0380017375.01 | MsG0680032521.01 | 0.800700 | 1.274571e-48 | 5.974060e-46 |
| MsG0580027361.01 | MsG0680032521.01 | 0.801666 | 8.086127e-49 | 3.883812e-46 |
| MsG0280007527.01 | MsG0680032521.01 | 0.814216 | 1.725105e-51 | 1.150532e-48 |
| MsG0280010411.01 | MsG0680032521.01 | 0.803865 | 2.842971e-49 | 1.444477e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032521.01.T01 | MTR_6g085190 | 99.474 | 190 | 1 | 0 | 1 | 190 | 450 | 639 | 2.37e-134 | 390 |
| MsG0680032521.01.T01 | MTR_4g127880 | 60.759 | 79 | 30 | 1 | 100 | 178 | 438 | 515 | 4.21e-26 | 104 |
| MsG0680032521.01.T01 | MTR_4g127880 | 60.759 | 79 | 30 | 1 | 100 | 178 | 621 | 698 | 4.70e-26 | 104 |
| MsG0680032521.01.T01 | MTR_4g127880 | 60.759 | 79 | 30 | 1 | 100 | 178 | 641 | 718 | 4.92e-26 | 104 |
| MsG0680032521.01.T01 | MTR_5g066440 | 57.143 | 70 | 30 | 0 | 104 | 173 | 513 | 582 | 1.61e-22 | 94.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032521.01.T01 | AT3G24440 | 43.814 | 194 | 70 | 6 | 1 | 181 | 428 | 595 | 8.30e-38 | 137 |
| MsG0680032521.01.T01 | AT3G24440 | 43.814 | 194 | 70 | 6 | 1 | 181 | 430 | 597 | 8.68e-38 | 137 |
| MsG0680032521.01.T01 | AT4G30200 | 55.172 | 87 | 37 | 1 | 95 | 181 | 612 | 696 | 3.11e-25 | 102 |
| MsG0680032521.01.T01 | AT4G30200 | 55.172 | 87 | 37 | 1 | 95 | 181 | 600 | 684 | 3.31e-25 | 102 |
| MsG0680032521.01.T01 | AT4G30200 | 55.172 | 87 | 37 | 1 | 95 | 181 | 583 | 667 | 3.51e-25 | 102 |
| MsG0680032521.01.T01 | AT2G18870 | 51.648 | 91 | 41 | 1 | 89 | 176 | 123 | 213 | 6.88e-21 | 87.0 |
| MsG0680032521.01.T01 | AT2G18880 | 50.667 | 75 | 37 | 0 | 107 | 181 | 440 | 514 | 1.75e-19 | 85.9 |
| MsG0680032521.01.T01 | AT5G57380 | 52.000 | 75 | 36 | 0 | 104 | 178 | 530 | 604 | 2.77e-18 | 82.4 |
| MsG0680032521.01.T01 | AT5G57380 | 52.000 | 75 | 36 | 0 | 104 | 178 | 510 | 584 | 2.88e-18 | 82.4 |
Find 45 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAAAGGAGAACCGTTTATTA+AGG | 0.300683 | 6:+43637841 | None:intergenic |
| TTCGTTTGGTCTCCAGGTTT+CGG | 0.314155 | 6:+43637955 | None:intergenic |
| AACTTCTTCGTTTGGTCTCC+AGG | 0.330854 | 6:+43637949 | None:intergenic |
| TCCATTTCTTGCCCTCTTGT+TGG | 0.338773 | 6:+43637580 | None:intergenic |
| ATACTCAAAGTTCTCATCTA+AGG | 0.365083 | 6:+43637796 | None:intergenic |
| AATTCGTTGGCTAGAATGTC+AGG | 0.388611 | 6:-43637762 | MsG0680032521.01.T01:CDS |
| CACTTTGCAGCAGGCTGTTG+AGG | 0.405269 | 6:-43638044 | MsG0680032521.01.T01:CDS |
| ACAGCAGGAACTTCTTCGTT+TGG | 0.411595 | 6:+43637941 | None:intergenic |
| GATCGATGATCCGAGTAGTT+TGG | 0.412460 | 6:-43637639 | MsG0680032521.01.T01:CDS |
| TGCTTACATGGTTTAGTTTG+AGG | 0.422999 | 6:-43637709 | MsG0680032521.01.T01:CDS |
| GCTTACATGGTTTAGTTTGA+GGG | 0.431261 | 6:-43637708 | MsG0680032521.01.T01:CDS |
| TCCAACAAGAGGGCAAGAAA+TGG | 0.444776 | 6:-43637581 | MsG0680032521.01.T01:CDS |
| TGTGACAGCACCTTAATAAA+CGG | 0.450247 | 6:-43637851 | MsG0680032521.01.T01:CDS |
| CGCTACCATGTGGTCTTGCT+AGG | 0.457631 | 6:+43637984 | None:intergenic |
| CAATGGTTGCACTTTGCAGC+AGG | 0.460748 | 6:-43638053 | MsG0680032521.01.T01:CDS |
| TTAGGCTGAAATTGCTTACA+TGG | 0.463255 | 6:-43637721 | MsG0680032521.01.T01:CDS |
| GTCATATGAAGAAAGAGTTT+AGG | 0.474263 | 6:-43637739 | MsG0680032521.01.T01:CDS |
| GTAGCGGTGGTTCCGAAACC+TGG | 0.474866 | 6:-43637967 | MsG0680032521.01.T01:CDS |
| GAACCATCGGGTGCATGTAA+AGG | 0.509944 | 6:+43637824 | None:intergenic |
| TGGATTCAATAACAAGGTTG+TGG | 0.533711 | 6:-43637561 | MsG0680032521.01.T01:CDS |
| TCTAAGGAGAACGAACCATC+GGG | 0.533883 | 6:+43637812 | None:intergenic |
| ATTGTGTTAAAGTAATTCGT+TGG | 0.547857 | 6:-43637775 | MsG0680032521.01.T01:CDS |
| ATGGTTTAGTTTGAGGGCAA+CGG | 0.555090 | 6:-43637702 | MsG0680032521.01.T01:CDS |
| TCATCCCGAGAAGACTCAAA+AGG | 0.555122 | 6:+43638079 | None:intergenic |
| GATGATCCGAGTAGTTTGGC+AGG | 0.560501 | 6:-43637635 | MsG0680032521.01.T01:CDS |
| CTGATATTATATCCAACAAG+AGG | 0.561241 | 6:-43637592 | MsG0680032521.01.T01:CDS |
| ATCTAAGGAGAACGAACCAT+CGG | 0.568116 | 6:+43637811 | None:intergenic |
| AGTTGACCTGCCAAACTACT+CGG | 0.568871 | 6:+43637629 | None:intergenic |
| GGCAACGGAACAAGAGCGTA+GGG | 0.584349 | 6:-43637687 | MsG0680032521.01.T01:CDS |
| GTTGACTCCCGCATAGTCGT+AGG | 0.596566 | 6:-43637920 | MsG0680032521.01.T01:CDS |
| TCTCCTTTACATGCACCCGA+TGG | 0.598572 | 6:-43637827 | MsG0680032521.01.T01:CDS |
| GGGCAACGGAACAAGAGCGT+AGG | 0.614096 | 6:-43637688 | MsG0680032521.01.T01:CDS |
| TGATATTATATCCAACAAGA+GGG | 0.623788 | 6:-43637591 | MsG0680032521.01.T01:CDS |
| ACTATGCGGGAGTCAACAGC+AGG | 0.624903 | 6:+43637926 | None:intergenic |
| ACTTGCACCTACGACTATGC+GGG | 0.634727 | 6:+43637913 | None:intergenic |
| CTAGCAAGACCACATGGTAG+CGG | 0.635274 | 6:-43637983 | MsG0680032521.01.T01:CDS |
| GCATAGTCGTAGGTGCAAGT+AGG | 0.636726 | 6:-43637910 | MsG0680032521.01.T01:CDS |
| GCAAGACCACATGGTAGCGG+TGG | 0.655229 | 6:-43637980 | MsG0680032521.01.T01:CDS |
| TCCCGAGAAGACTCAAAAGG+AGG | 0.665620 | 6:+43638082 | None:intergenic |
| TACTTGCACCTACGACTATG+CGG | 0.668138 | 6:+43637912 | None:intergenic |
| ATTCGTTGGCTAGAATGTCA+GGG | 0.682204 | 6:-43637761 | MsG0680032521.01.T01:CDS |
| AAGAAATGGATTCAATAACA+AGG | 0.687617 | 6:-43637567 | MsG0680032521.01.T01:CDS |
| TCTTCTCGGGATGAAGACAA+TGG | 0.691060 | 6:-43638070 | MsG0680032521.01.T01:CDS |
| TCGGAACCACCGCTACCATG+TGG | 0.691197 | 6:+43637974 | None:intergenic |
| AACGGAACAAGAGCGTAGGG+TGG | 0.711248 | 6:-43637684 | MsG0680032521.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATTGTGTTAAAGTAATTCGT+TGG | - | Chr6:43637875-43637894 | MsG0680032521.01.T01:CDS | 25.0% |
| ! | TGATATTATATCCAACAAGA+GGG | - | Chr6:43638059-43638078 | MsG0680032521.01.T01:CDS | 25.0% |
| !! | AAGAAATGGATTCAATAACA+AGG | - | Chr6:43638083-43638102 | MsG0680032521.01.T01:CDS | 25.0% |
| ATACTCAAAGTTCTCATCTA+AGG | + | Chr6:43637857-43637876 | None:intergenic | 30.0% | |
| CTGATATTATATCCAACAAG+AGG | - | Chr6:43638058-43638077 | MsG0680032521.01.T01:CDS | 30.0% | |
| GTCATATGAAGAAAGAGTTT+AGG | - | Chr6:43637911-43637930 | MsG0680032521.01.T01:CDS | 30.0% | |
| TAAAGGAGAACCGTTTATTA+AGG | + | Chr6:43637812-43637831 | None:intergenic | 30.0% | |
| ! | GTTTTTCTCTAAATCATGAG+AGG | + | Chr6:43637647-43637666 | None:intergenic | 30.0% |
| TGGATTCAATAACAAGGTTG+TGG | - | Chr6:43638089-43638108 | MsG0680032521.01.T01:CDS | 35.0% | |
| TGTGACAGCACCTTAATAAA+CGG | - | Chr6:43637799-43637818 | MsG0680032521.01.T01:CDS | 35.0% | |
| TTAGGCTGAAATTGCTTACA+TGG | - | Chr6:43637929-43637948 | MsG0680032521.01.T01:CDS | 35.0% | |
| !! | GCTTACATGGTTTAGTTTGA+GGG | - | Chr6:43637942-43637961 | MsG0680032521.01.T01:CDS | 35.0% |
| !! | TGCTTACATGGTTTAGTTTG+AGG | - | Chr6:43637941-43637960 | MsG0680032521.01.T01:CDS | 35.0% |
| AAAAACCTAGCAAGACCACA+TGG | - | Chr6:43637661-43637680 | MsG0680032521.01.T01:CDS | 40.0% | |
| ATCTAAGGAGAACGAACCAT+CGG | + | Chr6:43637842-43637861 | None:intergenic | 40.0% | |
| ! | AATTCGTTGGCTAGAATGTC+AGG | - | Chr6:43637888-43637907 | MsG0680032521.01.T01:CDS | 40.0% |
| ! | ATTCGTTGGCTAGAATGTCA+GGG | - | Chr6:43637889-43637908 | MsG0680032521.01.T01:CDS | 40.0% |
| !! | ATGGTTTAGTTTGAGGGCAA+CGG | - | Chr6:43637948-43637967 | MsG0680032521.01.T01:CDS | 40.0% |
| AACTTCTTCGTTTGGTCTCC+AGG | + | Chr6:43637704-43637723 | None:intergenic | 45.0% | |
| ACAGCAGGAACTTCTTCGTT+TGG | + | Chr6:43637712-43637731 | None:intergenic | 45.0% | |
| AGTTGACCTGCCAAACTACT+CGG | + | Chr6:43638024-43638043 | None:intergenic | 45.0% | |
| GATCGATGATCCGAGTAGTT+TGG | - | Chr6:43638011-43638030 | MsG0680032521.01.T01:CDS | 45.0% | |
| TACTTGCACCTACGACTATG+CGG | + | Chr6:43637741-43637760 | None:intergenic | 45.0% | |
| TCATCCCGAGAAGACTCAAA+AGG | + | Chr6:43637574-43637593 | None:intergenic | 45.0% | |
| TCCAACAAGAGGGCAAGAAA+TGG | - | Chr6:43638069-43638088 | MsG0680032521.01.T01:CDS | 45.0% | |
| TCCATTTCTTGCCCTCTTGT+TGG | + | Chr6:43638073-43638092 | None:intergenic | 45.0% | |
| TCTAAGGAGAACGAACCATC+GGG | + | Chr6:43637841-43637860 | None:intergenic | 45.0% | |
| TCTTCTCGGGATGAAGACAA+TGG | - | Chr6:43637580-43637599 | MsG0680032521.01.T01:CDS | 45.0% | |
| TTCGTTTGGTCTCCAGGTTT+CGG | + | Chr6:43637698-43637717 | None:intergenic | 45.0% | |
| !! | CTCCTCCTTTTGAGTCTTCT+CGG | - | Chr6:43637566-43637585 | MsG0680032521.01.T01:CDS | 45.0% |
| !! | TCCTCCTTTTGAGTCTTCTC+GGG | - | Chr6:43637567-43637586 | MsG0680032521.01.T01:CDS | 45.0% |
| ACTTGCACCTACGACTATGC+GGG | + | Chr6:43637740-43637759 | None:intergenic | 50.0% | |
| CAATGGTTGCACTTTGCAGC+AGG | - | Chr6:43637597-43637616 | MsG0680032521.01.T01:CDS | 50.0% | |
| CTAGCAAGACCACATGGTAG+CGG | - | Chr6:43637667-43637686 | MsG0680032521.01.T01:CDS | 50.0% | |
| GAACCATCGGGTGCATGTAA+AGG | + | Chr6:43637829-43637848 | None:intergenic | 50.0% | |
| GATGATCCGAGTAGTTTGGC+AGG | - | Chr6:43638015-43638034 | MsG0680032521.01.T01:CDS | 50.0% | |
| GCATAGTCGTAGGTGCAAGT+AGG | - | Chr6:43637740-43637759 | MsG0680032521.01.T01:CDS | 50.0% | |
| TCCCGAGAAGACTCAAAAGG+AGG | + | Chr6:43637571-43637590 | None:intergenic | 50.0% | |
| TCTCCTTTACATGCACCCGA+TGG | - | Chr6:43637823-43637842 | MsG0680032521.01.T01:CDS | 50.0% | |
| ACTATGCGGGAGTCAACAGC+AGG | + | Chr6:43637727-43637746 | None:intergenic | 55.0% | |
| CACTTTGCAGCAGGCTGTTG+AGG | - | Chr6:43637606-43637625 | MsG0680032521.01.T01:CDS | 55.0% | |
| ! | AACGGAACAAGAGCGTAGGG+TGG | - | Chr6:43637966-43637985 | MsG0680032521.01.T01:CDS | 55.0% |
| ! | CGCTACCATGTGGTCTTGCT+AGG | + | Chr6:43637669-43637688 | None:intergenic | 55.0% |
| ! | GGCAACGGAACAAGAGCGTA+GGG | - | Chr6:43637963-43637982 | MsG0680032521.01.T01:CDS | 55.0% |
| ! | GTTGACTCCCGCATAGTCGT+AGG | - | Chr6:43637730-43637749 | MsG0680032521.01.T01:CDS | 55.0% |
| GTAGCGGTGGTTCCGAAACC+TGG | - | Chr6:43637683-43637702 | MsG0680032521.01.T01:CDS | 60.0% | |
| TCGGAACCACCGCTACCATG+TGG | + | Chr6:43637679-43637698 | None:intergenic | 60.0% | |
| ! | GGGCAACGGAACAAGAGCGT+AGG | - | Chr6:43637962-43637981 | MsG0680032521.01.T01:CDS | 60.0% |
| !! | GCAAGACCACATGGTAGCGG+TGG | - | Chr6:43637670-43637689 | MsG0680032521.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 43637550 | 43638122 | 43637550 | ID=MsG0680032521.01;Name=MsG0680032521.01 |
| Chr6 | mRNA | 43637550 | 43638122 | 43637550 | ID=MsG0680032521.01.T01;Parent=MsG0680032521.01;Name=MsG0680032521.01.T01;_AED=0.25;_eAED=0.25;_QI=0|-1|0|1|-1|1|1|0|190 |
| Chr6 | exon | 43637550 | 43638122 | 43637550 | ID=MsG0680032521.01.T01:exon:11644;Parent=MsG0680032521.01.T01 |
| Chr6 | CDS | 43637550 | 43638122 | 43637550 | ID=MsG0680032521.01.T01:cds;Parent=MsG0680032521.01.T01 |
| Gene Sequence |
| Protein sequence |
