AlfalfaGEDB | MsG0880043081.01

M. sativa cultivar ZhongmuNo.1 / MsG0880043081.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880043081.01.T01	XP_024628287.1	97.531	243	6	0	1	243	1	243	9.34E-168	473

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880043081.01.T01	A0A396GM52	97.531	243	6	0	1	243	1	243	4.46e-168	473

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048784.01	MsG0880043081.01	0.844054	9.588123e-59	1.529575e-55
MsG0080048815.01	MsG0880043081.01	0.803264	3.787360e-49	1.894739e-46
MsG0080048856.01	MsG0880043081.01	0.841763	3.900699e-58	5.789796e-55
MsG0180000139.01	MsG0880043081.01	0.830339	3.105335e-55	3.262429e-52
MsG0180000144.01	MsG0880043081.01	0.818939	1.508815e-52	1.145293e-49
MsG0180000155.01	MsG0880043081.01	0.840535	8.200798e-58	1.171664e-54
MsG0180000235.01	MsG0880043081.01	0.807019	6.199868e-50	3.415844e-47
MsG0180000568.01	MsG0880043081.01	0.800240	1.581161e-48	7.325374e-46
MsG0180000835.01	MsG0880043081.01	0.815003	1.154863e-51	7.868351e-49
MsG0180000836.01	MsG0880043081.01	0.840847	6.794238e-58	9.801691e-55
MsG0180001173.01	MsG0880043081.01	0.809409	1.919928e-50	1.126407e-47
MsG0180002547.01	MsG0880043081.01	0.849819	2.531572e-60	4.859257e-57
MsG0180002794.01	MsG0880043081.01	0.821849	3.244752e-53	2.670715e-50
MsG0180003493.01	MsG0880043081.01	0.809868	1.529236e-50	9.081520e-48
MsG0180003932.01	MsG0880043081.01	0.809742	1.627602e-50	9.634238e-48
MsG0180004062.01	MsG0880043081.01	0.830909	2.251239e-55	2.405131e-52
MsG0180004152.01	MsG0880043081.01	0.818293	2.114496e-52	1.576407e-49
MsG0180004389.01	MsG0880043081.01	0.812247	4.666928e-51	2.952227e-48
MsG0180004481.01	MsG0880043081.01	0.833332	5.667876e-56	6.507164e-53
MsG0180004482.01	MsG0880043081.01	0.833615	4.818156e-56	5.577832e-53
MsG0180004509.01	MsG0880043081.01	0.804720	1.886902e-49	9.800404e-47
MsG0180004725.01	MsG0880043081.01	0.820136	8.044446e-53	6.312735e-50
MsG0180004769.01	MsG0880043081.01	0.815299	9.926526e-52	6.817675e-49
MsG0180004928.01	MsG0880043081.01	0.822995	1.757751e-53	1.494717e-50
MsG0180005439.01	MsG0880043081.01	0.802804	4.715091e-49	2.331425e-46
MsG0180005445.01	MsG0880043081.01	0.810220	1.284196e-50	7.696383e-48
MsG0180005575.01	MsG0880043081.01	0.822846	1.903566e-53	1.611942e-50
MsG0180005746.01	MsG0880043081.01	0.833582	4.909723e-56	5.678460e-53
MsG0180006017.01	MsG0880043081.01	0.829387	5.296283e-55	5.412909e-52
MsG0180006070.01	MsG0880043081.01	0.818670	1.736558e-52	1.308216e-49
MsG0180006155.01	MsG0880043081.01	0.842552	2.411299e-58	3.669275e-55
MsG0280006305.01	MsG0880043081.01	0.818746	1.668760e-52	1.259789e-49
MsG0280006405.01	MsG0880043081.01	0.824146	9.455026e-54	8.305546e-51
MsG0780040384.01	MsG0880043081.01	0.848675	5.268541e-60	9.743602e-57
MsG0780040734.01	MsG0880043081.01	0.824347	8.478338e-54	7.489774e-51
MsG0780041258.01	MsG0880043081.01	0.809995	1.436041e-50	8.556853e-48
MsG0780041268.01	MsG0880043081.01	0.818976	1.479695e-52	1.124346e-49
MsG0780041305.01	MsG0880043081.01	0.828735	7.621314e-55	7.640223e-52
MsG0780041559.01	MsG0880043081.01	0.808740	2.670001e-50	1.538626e-47
MsG0780041667.01	MsG0880043081.01	0.802616	5.156416e-49	2.537344e-46
MsG0880042324.01	MsG0880043081.01	0.847062	1.466215e-59	2.574936e-56
MsG0880042519.01	MsG0880043081.01	0.816733	4.749566e-52	3.392699e-49
MsG0880042760.01	MsG0880043081.01	0.811096	8.304532e-51	5.094455e-48
MsG0880043081.01	MsG0880044565.01	0.823221	1.556605e-53	1.332093e-50
MsG0880043081.01	MsG0880045145.01	0.821745	3.429571e-53	2.814207e-50
MsG0880043081.01	MsG0880045294.01	0.861719	8.421993e-64	2.404966e-60
MsG0880043081.01	MsG0880045372.01	0.803463	3.444217e-49	1.731940e-46
MsG0880043081.01	MsG0880045393.01	0.807704	4.437307e-50	2.488672e-47
MsG0880043081.01	MsG0880045535.01	0.806660	7.383344e-50	4.030116e-47
MsG0880043081.01	MsG0880045656.01	0.817562	3.092566e-52	2.259795e-49
MsG0880043081.01	MsG0880045684.01	0.806501	7.977706e-50	4.337033e-47
MsG0880043081.01	MsG0880047470.01	0.819096	1.389684e-52	1.059299e-49
MsG0880043081.01	MsG0880047472.01	0.844432	7.586888e-59	1.224587e-55
MsG0880043081.01	MsG0880047473.01	0.829725	4.384428e-55	4.525441e-52
MsG0280010776.01	MsG0880043081.01	0.801311	9.561355e-49	4.551597e-46
MsG0280010778.01	MsG0880043081.01	0.847117	1.416721e-59	2.492509e-56
MsG0280011014.01	MsG0880043081.01	0.808009	3.823359e-50	2.161523e-47
MsG0280011339.01	MsG0880043081.01	0.820091	8.238097e-53	6.456739e-50
MsG0280011340.01	MsG0880043081.01	0.811685	6.186873e-51	3.854587e-48
MsG0380012103.01	MsG0880043081.01	0.814371	1.593737e-51	1.067466e-48
MsG0380012460.01	MsG0880043081.01	0.810374	1.189968e-50	7.160724e-48
MsG0380013183.01	MsG0880043081.01	0.805171	1.518678e-49	7.979669e-47
MsG0380014489.01	MsG0880043081.01	0.803655	3.142380e-49	1.587912e-46
MsG0380014703.01	MsG0880043081.01	0.807104	5.948225e-50	3.284280e-47
MsG0380014875.01	MsG0880043081.01	0.831072	2.053019e-55	2.203844e-52
MsG0680031214.01	MsG0880043081.01	0.822498	2.293785e-53	1.923245e-50
MsG0680031506.01	MsG0880043081.01	0.845833	3.174036e-59	5.358071e-56
MsG0680031507.01	MsG0880043081.01	0.848033	7.930841e-60	1.436348e-56
MsG0680031628.01	MsG0880043081.01	0.813615	2.340619e-51	1.535903e-48
MsG0680031699.01	MsG0880043081.01	0.814221	1.720563e-51	1.147670e-48
MsG0680032521.01	MsG0880043081.01	0.805233	1.473799e-49	7.756160e-47
MsG0680034853.01	MsG0880043081.01	0.819292	1.253921e-52	9.609544e-50
MsG0680034913.01	MsG0880043081.01	0.849524	3.060364e-60	5.817243e-57
MsG0680035306.01	MsG0880043081.01	0.800509	1.394272e-48	6.503663e-46
MsG0680035577.01	MsG0880043081.01	0.820615	6.247923e-53	4.967656e-50
MsG0480019539.01	MsG0880043081.01	0.833268	5.878542e-56	6.735398e-53
MsG0480020447.01	MsG0880043081.01	0.802588	5.223769e-49	2.568678e-46
MsG0480020675.01	MsG0880043081.01	0.804296	2.312440e-49	1.187961e-46
MsG0480020739.01	MsG0880043081.01	0.838892	2.194432e-57	2.980130e-54
MsG0480020879.01	MsG0880043081.01	0.814386	1.581872e-51	1.059983e-48
MsG0480021037.01	MsG0880043081.01	0.803671	3.118831e-49	1.576646e-46
MsG0480021409.01	MsG0880043081.01	0.815160	1.065909e-51	7.292681e-49
MsG0480021465.01	MsG0880043081.01	0.820569	6.401660e-53	5.083446e-50
MsG0480021501.01	MsG0880043081.01	0.826609	2.470291e-54	2.327840e-51
MsG0480021706.01	MsG0880043081.01	0.826877	2.131873e-54	2.024600e-51
MsG0480021724.01	MsG0880043081.01	0.815171	1.059950e-51	7.253910e-49
MsG0480022164.01	MsG0880043081.01	0.812662	3.786764e-51	2.421972e-48
MsG0480022318.01	MsG0880043081.01	0.800472	1.418372e-48	6.609875e-46
MsG0480022549.01	MsG0880043081.01	0.807161	5.787209e-50	3.200172e-47
MsG0480022696.01	MsG0880043081.01	0.827178	1.805561e-54	1.729981e-51
MsG0480022711.01	MsG0880043081.01	0.832001	1.212600e-55	1.338073e-52
MsG0480023055.01	MsG0880043081.01	0.831506	1.605870e-55	1.746221e-52
MsG0480023221.01	MsG0880043081.01	0.826624	2.449867e-54	2.309664e-51
MsG0480023354.01	MsG0880043081.01	0.825055	5.773229e-54	5.205436e-51
MsG0480023356.01	MsG0880043081.01	0.807989	3.860108e-50	2.181172e-47
MsG0480023387.01	MsG0880043081.01	0.813413	2.592202e-51	1.691729e-48
MsG0480023463.01	MsG0880043081.01	0.846423	2.192045e-59	3.771344e-56
MsG0480023600.01	MsG0880043081.01	0.816118	6.521706e-52	4.580987e-49
MsG0580024197.01	MsG0880043081.01	0.842900	1.949342e-58	2.998526e-55
MsG0580024536.01	MsG0880043081.01	0.806178	9.331736e-50	5.031738e-47
MsG0580025268.01	MsG0880043081.01	0.800451	1.432690e-48	6.673084e-46
MsG0580025332.01	MsG0880043081.01	0.806174	9.350241e-50	5.041153e-47
MsG0580025439.01	MsG0880043081.01	0.832475	9.253988e-56	1.035484e-52
MsG0580025587.01	MsG0880043081.01	0.807660	4.535845e-50	2.540922e-47
MsG0580025651.01	MsG0880043081.01	0.810286	1.242857e-50	7.461542e-48
MsG0380015332.01	MsG0880043081.01	0.818167	2.257838e-52	1.677435e-49
MsG0380015440.01	MsG0880043081.01	0.838423	2.902046e-57	3.885150e-54
MsG0380015444.01	MsG0880043081.01	0.805789	1.126710e-49	6.015022e-47
MsG0380015463.01	MsG0880043081.01	0.818830	1.597038e-52	1.208480e-49
MsG0380015555.01	MsG0880043081.01	0.803521	3.351282e-49	1.687732e-46
MsG0380015831.01	MsG0880043081.01	0.824327	8.572543e-54	7.568716e-51
MsG0380016005.01	MsG0880043081.01	0.864632	1.057602e-64	3.343986e-61
MsG0380016067.01	MsG0880043081.01	0.838742	2.400294e-57	3.244882e-54
MsG0380016513.01	MsG0880043081.01	0.804039	2.614946e-49	1.334571e-46
MsG0380016860.01	MsG0880043081.01	0.802939	4.421023e-49	2.193411e-46
MsG0380016970.01	MsG0880043081.01	0.857134	2.007561e-62	4.904749e-59
MsG0380017375.01	MsG0880043081.01	0.807838	4.156680e-50	2.339420e-47
MsG0480018163.01	MsG0880043081.01	0.805919	1.058156e-49	5.667817e-47
MsG0580026137.01	MsG0880043081.01	0.810840	9.436241e-51	5.749644e-48
MsG0580027361.01	MsG0880043081.01	0.803391	3.565313e-49	1.789444e-46
MsG0580027891.01	MsG0880043081.01	0.811581	6.519021e-51	4.050482e-48
MsG0580029008.01	MsG0880043081.01	0.806472	8.089744e-50	4.394821e-47
MsG0580029647.01	MsG0880043081.01	0.803212	3.882971e-49	1.939962e-46
MsG0580029651.01	MsG0880043081.01	0.801689	7.998793e-49	3.844057e-46
MsG0580030142.01	MsG0880043081.01	0.801735	7.827172e-49	3.765956e-46
MsG0280006809.01	MsG0880043081.01	0.806674	7.333777e-50	4.004517e-47
MsG0280006852.01	MsG0880043081.01	0.812983	3.220586e-51	2.077337e-48
MsG0280006930.01	MsG0880043081.01	0.821793	3.341776e-53	2.746162e-50
MsG0280007222.01	MsG0880043081.01	0.817368	3.420183e-52	2.485963e-49
MsG0280007452.01	MsG0880043081.01	0.819614	1.059083e-52	8.189611e-50
MsG0280007527.01	MsG0880043081.01	0.875441	3.074628e-68	1.435539e-64
MsG0280007955.01	MsG0880043081.01	0.804443	2.154501e-49	1.110994e-46
MsG0280008457.01	MsG0880043081.01	0.803388	3.569600e-49	1.791472e-46
MsG0280008696.01	MsG0880043081.01	0.840846	6.799407e-58	9.808899e-55
MsG0280009544.01	MsG0880043081.01	0.852421	4.668451e-61	9.749009e-58
MsG0280009575.01	MsG0880043081.01	0.829642	4.591205e-55	4.727221e-52
MsG0280010184.01	MsG0880043081.01	0.802871	4.566368e-49	2.261706e-46
MsG0280010233.01	MsG0880043081.01	0.812745	3.631427e-51	2.327725e-48
MsG0280010287.01	MsG0880043081.01	0.800351	1.501569e-48	6.976294e-46
MsG0280010411.01	MsG0880043081.01	0.818265	2.145171e-52	1.598018e-49
MsG0280010533.01	MsG0880043081.01	0.802011	6.868677e-49	3.328168e-46
MsG0680035876.01	MsG0880043081.01	0.818163	2.262286e-52	1.680591e-49
MsG0680035888.01	MsG0880043081.01	0.833574	4.932883e-56	5.703703e-53
MsG0780037240.01	MsG0880043081.01	0.801228	9.939890e-49	4.721873e-46
MsG0780038401.01	MsG0880043081.01	0.815948	7.117483e-52	4.975998e-49
MsG0780038565.01	MsG0880043081.01	0.822784	1.967925e-53	1.663489e-50
MsG0780038603.01	MsG0880043081.01	0.849500	3.107029e-60	5.901771e-57
MsG0780039443.01	MsG0880043081.01	0.812701	3.714238e-51	2.378002e-48
MsG0780040057.01	MsG0880043081.01	0.817346	3.459351e-52	2.512889e-49

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880043081.01.T01	MTR_8g023960	95.473	243	8	1	1	243	57	296	4.63e-167	463
MsG0880043081.01.T01	MTR_8g023960	95.473	243	8	1	1	243	1	240	3.37e-166	458

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880043081.01.T01	AT4G05400	56.078	255	95	5	1	243	1	250	8.50e-88	260
MsG0880043081.01.T01	AT4G05400	56.078	255	95	5	1	243	1	250	8.50e-88	260
MsG0880043081.01.T01	AT4G05400	56.078	255	95	5	1	243	1	250	8.50e-88	260
MsG0880043081.01.T01	AT4G21140	54.656	247	104	5	1	243	1	243	1.10e-74	227

Find 76 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACCAGTTGGGGCACCGGGTG+TGG	0.216920	8:-18654486	None:intergenic
GCCGGATTTGACTCCGTTTC+CGG	0.238916	8:+18654934	MsG0880043081.01.T01:CDS
GTTTCCGGTGAATAGGTTTA+TGG	0.264318	8:+18654949	MsG0880043081.01.T01:CDS
GGGTTGGATTATATAACATT+AGG	0.279672	8:+18654722	MsG0880043081.01.T01:CDS
TCTTTCGTCAATTCATATTT+CGG	0.289163	8:-18654767	None:intergenic
AGCTTCGCGTGCACGTTGTT+TGG	0.290006	8:-18654585	None:intergenic
TTCTGAGAAGCCAACGATAA+TGG	0.299403	8:+18654691	MsG0880043081.01.T01:CDS
TGGGGCACCGGGTGTGGGTT+CGG	0.342298	8:-18654480	None:intergenic
GAAAGAAGATGGGATGAAAT+TGG	0.349722	8:+18654784	MsG0880043081.01.T01:CDS
GGTTGGATTATATAACATTA+GGG	0.357133	8:+18654723	MsG0880043081.01.T01:CDS
GCTTCGCGTGCACGTTGTTT+GGG	0.366189	8:-18654584	None:intergenic
GTTTCTCGCGGGAACCAGTT+GGG	0.371311	8:-18654499	None:intergenic
TCAGTGGGAAGGAGAAGATT+AGG	0.382186	8:+18655032	MsG0880043081.01.T01:CDS
AGGTTTGAAGGAAGCTGCTT+TGG	0.388226	8:+18654910	MsG0880043081.01.T01:CDS
TGACTCCGTTTCCGGTGAAT+AGG	0.396696	8:+18654942	MsG0880043081.01.T01:CDS
ACCGGGTGTGGGTTCGGATC+CGG	0.397530	8:-18654474	None:intergenic
TCTGAGAAGCCAACGATAAT+GGG	0.401787	8:+18654692	MsG0880043081.01.T01:CDS
CCCACACCCGGTGCCCCAAC+TGG	0.402280	8:+18654485	MsG0880043081.01.T01:CDS
TTATATAACATTAGGGTTAG+TGG	0.413282	8:+18654730	MsG0880043081.01.T01:CDS
AGCCGCACCGCAAAAGGAAA+AGG	0.413446	8:+18654404	MsG0880043081.01.T01:CDS
GCGGGAACCAGTTGGGGCAC+CGG	0.419435	8:-18654492	None:intergenic
GGGGAGGATGACAGTGGTGA+TGG	0.423787	8:-18654353	None:intergenic
AATTGGCCAAGGAGTATAGT+AGG	0.424942	8:+18654801	MsG0880043081.01.T01:CDS
TCCGTCAGCCGCACCGCAAA+AGG	0.432750	8:+18654398	MsG0880043081.01.T01:CDS
CGGGAACCAGTTGGGGCACC+GGG	0.441391	8:-18654491	None:intergenic
TATGAATTGACGAAAGAAGA+TGG	0.442226	8:+18654773	MsG0880043081.01.T01:CDS
TGGGTATATTGAGAAGATTA+AGG	0.447659	8:+18654994	MsG0880043081.01.T01:CDS
AGCTTTACCTGGAGGTTTGA+AGG	0.450403	8:+18654898	MsG0880043081.01.T01:CDS
CCAGTTGGGGCACCGGGTGT+GGG	0.450669	8:-18654485	None:intergenic
TGTTTCTCGCGGGAACCAGT+TGG	0.454283	8:-18654500	None:intergenic
ATAAACCTATTCACCGGAAA+CGG	0.456384	8:-18654947	None:intergenic
TGCGGCTGACGGAGCAGCGT+TGG	0.460867	8:-18654388	None:intergenic
AGATTAAGGAGGCTGCTGAT+AGG	0.463125	8:+18655008	MsG0880043081.01.T01:CDS
CGGAGCAGCGTTGGAGGAGT+TGG	0.464939	8:-18654379	None:intergenic
TTCTCAATATACCCATCAAT+AGG	0.473537	8:-18654986	None:intergenic
GAAGGAAGCTGCTTTGGTGC+CGG	0.473891	8:+18654916	MsG0880043081.01.T01:CDS
AGCGTTGGAGGAGTTGGTGT+GGG	0.476604	8:-18654373	None:intergenic
GAGGCAATTGAAGCTTTACC+TGG	0.482431	8:+18654887	MsG0880043081.01.T01:CDS
GGCTGACGGAGCAGCGTTGG+AGG	0.488541	8:-18654385	None:intergenic
GAGGATGACAGTGGTGATGG+TGG	0.488777	8:-18654350	None:intergenic
ACCGGAAACGGAGTCAAATC+CGG	0.495763	8:-18654935	None:intergenic
CCAACGATAATGGGAACACC+TGG	0.497189	8:+18654701	MsG0880043081.01.T01:CDS
ACGCTTACGCCACCTATTGA+TGG	0.498561	8:+18654974	MsG0880043081.01.T01:CDS
CCAGGTGTTCCCATTATCGT+TGG	0.510994	8:-18654701	None:intergenic
TTCGCGTTCTTGTTTCTCGC+GGG	0.513895	8:-18654510	None:intergenic
CAGCGTTGGAGGAGTTGGTG+TGG	0.521072	8:-18654374	None:intergenic
ACCGGATCCGAACCCACACC+CGG	0.521607	8:+18654473	MsG0880043081.01.T01:CDS
ATGAATTGACGAAAGAAGAT+GGG	0.521993	8:+18654774	MsG0880043081.01.T01:CDS
TTTCTCGCGGGAACCAGTTG+GGG	0.524113	8:-18654498	None:intergenic
GCCGCACCGCAAAAGGAAAA+GGG	0.525322	8:+18654405	MsG0880043081.01.T01:CDS
TTGGAGGAGTTGGTGTGGGG+AGG	0.529803	8:-18654369	None:intergenic
CGCTTACGCCACCTATTGAT+GGG	0.534577	8:+18654975	MsG0880043081.01.T01:CDS
GCAGCTTCCTTCAAACCTCC+AGG	0.536551	8:-18654905	None:intergenic
GATAATGGGAACACCTGGGT+TGG	0.543156	8:+18654706	MsG0880043081.01.T01:CDS
GTTCGCGTTCTTGTTTCTCG+CGG	0.547502	8:-18654511	None:intergenic
GCAAAAGGAAAAGGGAAAGC+CGG	0.550247	8:+18654413	MsG0880043081.01.T01:CDS
CAAAACCCTACTATACTCCT+TGG	0.553845	8:-18654807	None:intergenic
TTGAGAAAACGAAGTGGTGT+TGG	0.571375	8:-18654560	None:intergenic
ATTGGCCAAGGAGTATAGTA+GGG	0.576553	8:+18654802	MsG0880043081.01.T01:CDS
GCGTTGGAGGAGTTGGTGTG+GGG	0.585225	8:-18654372	None:intergenic
GAAGCATAGGGCGAGACAAG+CGG	0.597439	8:+18654835	MsG0880043081.01.T01:CDS
TGCTGATAGGATCAGTGGGA+AGG	0.607298	8:+18655021	MsG0880043081.01.T01:CDS
GAGGCTGCTGATAGGATCAG+TGG	0.610487	8:+18655016	MsG0880043081.01.T01:CDS
GTCGCCATAAACCTATTCAC+CGG	0.613259	8:-18654953	None:intergenic
AGGCTGCTGATAGGATCAGT+GGG	0.618985	8:+18655017	MsG0880043081.01.T01:CDS
TGGTGTGGGGAGGATGACAG+TGG	0.627178	8:-18654359	None:intergenic
AGATGGGATGAAATTGGCCA+AGG	0.633058	8:+18654790	MsG0880043081.01.T01:CDS
TCAATATACCCATCAATAGG+TGG	0.649441	8:-18654983	None:intergenic
GCATAGGGCGAGACAAGCGG+CGG	0.658080	8:+18654838	MsG0880043081.01.T01:CDS
GCAATTGAAGCTTTACCTGG+AGG	0.659327	8:+18654890	MsG0880043081.01.T01:CDS
CGTTGAGGCATTGATCGTAG+AGG	0.672109	8:-18654535	None:intergenic
GTATATTGAGAAGATTAAGG+AGG	0.685736	8:+18654997	MsG0880043081.01.T01:CDS
AATGTTATATAATCCAACCC+AGG	0.686284	8:-18654719	None:intergenic
CAACGATAATGGGAACACCT+GGG	0.706454	8:+18654702	MsG0880043081.01.T01:CDS
TTGGGTTTGAGAAAACGAAG+TGG	0.743892	8:-18654566	None:intergenic
GAAGTGGTGTTGGTGCGTTG+AGG	0.759588	8:-18654550	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAAATCACTGTCAAAAAAAC+CGG	+	Chr8:18654455-18654474	MsG0880043081.01.T01:CDS	25.0%
!	GGTTGGATTATATAACATTA+GGG	+	Chr8:18654723-18654742	MsG0880043081.01.T01:CDS	25.0%
!	TCTTTCGTCAATTCATATTT+CGG	-	Chr8:18654770-18654789	None:intergenic	25.0%
!	TTATATAACATTAGGGTTAG+TGG	+	Chr8:18654730-18654749	MsG0880043081.01.T01:CDS	25.0%
	AAGCTGAACGAGAAAAATTA+GGG	+	Chr8:18654603-18654622	MsG0880043081.01.T01:CDS	30.0%
	AATGTTATATAATCCAACCC+AGG	-	Chr8:18654722-18654741	None:intergenic	30.0%
	ATGAATTGACGAAAGAAGAT+GGG	+	Chr8:18654774-18654793	MsG0880043081.01.T01:CDS	30.0%
	GGGTTGGATTATATAACATT+AGG	+	Chr8:18654722-18654741	MsG0880043081.01.T01:CDS	30.0%
	GTATATTGAGAAGATTAAGG+AGG	+	Chr8:18654997-18655016	MsG0880043081.01.T01:CDS	30.0%
	TATGAATTGACGAAAGAAGA+TGG	+	Chr8:18654773-18654792	MsG0880043081.01.T01:CDS	30.0%
	TGGGTATATTGAGAAGATTA+AGG	+	Chr8:18654994-18655013	MsG0880043081.01.T01:CDS	30.0%
	TTCTCAATATACCCATCAAT+AGG	-	Chr8:18654989-18655008	None:intergenic	30.0%
!!	GAATCTTTTGAGGATGAAAA+AGG	+	Chr8:18654865-18654884	MsG0880043081.01.T01:CDS	30.0%
!!!	GGTTTTTTTGACAGTGATTT+TGG	-	Chr8:18654456-18654475	None:intergenic	30.0%
	AGAAGAAAAACGCGAAGTTT+AGG	+	Chr8:18654666-18654685	MsG0880043081.01.T01:CDS	35.0%
	ATAAACCTATTCACCGGAAA+CGG	-	Chr8:18654950-18654969	None:intergenic	35.0%
	GAAAGAAGATGGGATGAAAT+TGG	+	Chr8:18654784-18654803	MsG0880043081.01.T01:CDS	35.0%
	GAAGAAAAACGCGAAGTTTA+GGG	+	Chr8:18654667-18654686	MsG0880043081.01.T01:CDS	35.0%
	GAAGCTGAACGAGAAAAATT+AGG	+	Chr8:18654602-18654621	MsG0880043081.01.T01:CDS	35.0%
	TCAATATACCCATCAATAGG+TGG	-	Chr8:18654986-18655005	None:intergenic	35.0%
!!	AGTATAGTAGGGTTTTGATG+AGG	+	Chr8:18654813-18654832	MsG0880043081.01.T01:CDS	35.0%
!!	TCTTTTGAGGATGAAAAAGG+AGG	+	Chr8:18654868-18654887	MsG0880043081.01.T01:CDS	35.0%
!!!	CAGTGATTTTGGAACGTTTT+AGG	-	Chr8:18654445-18654464	None:intergenic	35.0%
	CAAAACCCTACTATACTCCT+TGG	-	Chr8:18654810-18654829	None:intergenic	40.0%
	GTTTCCGGTGAATAGGTTTA+TGG	+	Chr8:18654949-18654968	MsG0880043081.01.T01:CDS	40.0%
	TCTGAGAAGCCAACGATAAT+GGG	+	Chr8:18654692-18654711	MsG0880043081.01.T01:CDS	40.0%
	TTGAGAAAACGAAGTGGTGT+TGG	-	Chr8:18654563-18654582	None:intergenic	40.0%
	TTGGGTTTGAGAAAACGAAG+TGG	-	Chr8:18654569-18654588	None:intergenic	40.0%
!	AATTGGCCAAGGAGTATAGT+AGG	+	Chr8:18654801-18654820	MsG0880043081.01.T01:CDS	40.0%
!	ATTGGCCAAGGAGTATAGTA+GGG	+	Chr8:18654802-18654821	MsG0880043081.01.T01:CDS	40.0%
!	TTCTGAGAAGCCAACGATAA+TGG	+	Chr8:18654691-18654710	MsG0880043081.01.T01:CDS	40.0%
!!	GGTTTTGATGAGGAAGCATA+GGG	+	Chr8:18654823-18654842	MsG0880043081.01.T01:CDS	40.0%
!!!	ATGGTGGTTTTAGCTTTGTG+AGG	-	Chr8:18654337-18654356	None:intergenic	40.0%
!!!	GGAACGTTTTAGGATTTGAC+CGG	-	Chr8:18654435-18654454	None:intergenic	40.0%
	AGATGGGATGAAATTGGCCA+AGG	+	Chr8:18654790-18654809	MsG0880043081.01.T01:CDS	45.0%
	AGATTAAGGAGGCTGCTGAT+AGG	+	Chr8:18655008-18655027	MsG0880043081.01.T01:CDS	45.0%
	AGCTTTACCTGGAGGTTTGA+AGG	+	Chr8:18654898-18654917	MsG0880043081.01.T01:CDS	45.0%
	AGGTTTGAAGGAAGCTGCTT+TGG	+	Chr8:18654910-18654929	MsG0880043081.01.T01:CDS	45.0%
	GAGGCAATTGAAGCTTTACC+TGG	+	Chr8:18654887-18654906	MsG0880043081.01.T01:CDS	45.0%
	GCAAAAGGAAAAGGGAAAGC+CGG	+	Chr8:18654413-18654432	MsG0880043081.01.T01:CDS	45.0%
	GCAATTGAAGCTTTACCTGG+AGG	+	Chr8:18654890-18654909	MsG0880043081.01.T01:CDS	45.0%
	GTCGCCATAAACCTATTCAC+CGG	-	Chr8:18654956-18654975	None:intergenic	45.0%
	TCAGTGGGAAGGAGAAGATT+AGG	+	Chr8:18655032-18655051	MsG0880043081.01.T01:CDS	45.0%
!	GGCTTTCCCTTTTCCTTTTG+CGG	-	Chr8:18654414-18654433	None:intergenic	45.0%
!!	CAACGATAATGGGAACACCT+GGG	+	Chr8:18654702-18654721	MsG0880043081.01.T01:CDS	45.0%
!!	GGGTTTTGATGAGGAAGCAT+AGG	+	Chr8:18654822-18654841	MsG0880043081.01.T01:CDS	45.0%
	ACCGGAAACGGAGTCAAATC+CGG	-	Chr8:18654938-18654957	None:intergenic	50.0%
	ACGCTTACGCCACCTATTGA+TGG	+	Chr8:18654974-18654993	MsG0880043081.01.T01:CDS	50.0%
	AGGCTGCTGATAGGATCAGT+GGG	+	Chr8:18655017-18655036	MsG0880043081.01.T01:CDS	50.0%
	CCAGGTGTTCCCATTATCGT+TGG	-	Chr8:18654704-18654723	None:intergenic	50.0%
	CGCTTACGCCACCTATTGAT+GGG	+	Chr8:18654975-18654994	MsG0880043081.01.T01:CDS	50.0%
	TGACTCCGTTTCCGGTGAAT+AGG	+	Chr8:18654942-18654961	MsG0880043081.01.T01:CDS	50.0%
	TGCTGATAGGATCAGTGGGA+AGG	+	Chr8:18655021-18655040	MsG0880043081.01.T01:CDS	50.0%
!	GTTCGCGTTCTTGTTTCTCG+CGG	-	Chr8:18654514-18654533	None:intergenic	50.0%
!	TCCCTTTTCCTTTTGCGGTG+CGG	-	Chr8:18654409-18654428	None:intergenic	50.0%
!	TTCGCGTTCTTGTTTCTCGC+GGG	-	Chr8:18654513-18654532	None:intergenic	50.0%
!!	CCAACGATAATGGGAACACC+TGG	+	Chr8:18654701-18654720	MsG0880043081.01.T01:CDS	50.0%
!!	CGTTGAGGCATTGATCGTAG+AGG	-	Chr8:18654538-18654557	None:intergenic	50.0%
!!	GATAATGGGAACACCTGGGT+TGG	+	Chr8:18654706-18654725	MsG0880043081.01.T01:CDS	50.0%
	AGCCGCACCGCAAAAGGAAA+AGG	+	Chr8:18654404-18654423	MsG0880043081.01.T01:CDS	55.0%
	AGCTTCGCGTGCACGTTGTT+TGG	-	Chr8:18654588-18654607	None:intergenic	55.0%
	GAAGCATAGGGCGAGACAAG+CGG	+	Chr8:18654835-18654854	MsG0880043081.01.T01:CDS	55.0%
	GAGGCTGCTGATAGGATCAG+TGG	+	Chr8:18655016-18655035	MsG0880043081.01.T01:CDS	55.0%
	GCAGCTTCCTTCAAACCTCC+AGG	-	Chr8:18654908-18654927	None:intergenic	55.0%
	GCCGCACCGCAAAAGGAAAA+GGG	+	Chr8:18654405-18654424	MsG0880043081.01.T01:CDS	55.0%
	GCCGGATTTGACTCCGTTTC+CGG	+	Chr8:18654934-18654953	MsG0880043081.01.T01:CDS	55.0%
	GCTTCGCGTGCACGTTGTTT+GGG	-	Chr8:18654587-18654606	None:intergenic	55.0%
	GTTTCTCGCGGGAACCAGTT+GGG	-	Chr8:18654502-18654521	None:intergenic	55.0%
	TGTTTCTCGCGGGAACCAGT+TGG	-	Chr8:18654503-18654522	None:intergenic	55.0%
	TTTCTCGCGGGAACCAGTTG+GGG	-	Chr8:18654501-18654520	None:intergenic	55.0%
!	CGGCGGAGACGAATCTTTTG+AGG	+	Chr8:18654855-18654874	MsG0880043081.01.T01:CDS	55.0%
!	GAAGGAAGCTGCTTTGGTGC+CGG	+	Chr8:18654916-18654935	MsG0880043081.01.T01:CDS	55.0%
!!	AGCGTTGGAGGAGTTGGTGT+GGG	-	Chr8:18654376-18654395	None:intergenic	55.0%
!!	GAAGTGGTGTTGGTGCGTTG+AGG	-	Chr8:18654553-18654572	None:intergenic	55.0%
!!	GAGGATGACAGTGGTGATGG+TGG	-	Chr8:18654353-18654372	None:intergenic	55.0%
	GGGGAGGATGACAGTGGTGA+TGG	-	Chr8:18654356-18654375	None:intergenic	60.0%
	TGGTGTGGGGAGGATGACAG+TGG	-	Chr8:18654362-18654381	None:intergenic	60.0%
!	TCCTTTTGCGGTGCGGCTGA+CGG	-	Chr8:18654402-18654421	None:intergenic	60.0%
!!	CAGCGTTGGAGGAGTTGGTG+TGG	-	Chr8:18654377-18654396	None:intergenic	60.0%
!!	GCGTTGGAGGAGTTGGTGTG+GGG	-	Chr8:18654375-18654394	None:intergenic	60.0%
!!	TTGGAGGAGTTGGTGTGGGG+AGG	-	Chr8:18654372-18654391	None:intergenic	60.0%
	ACCGGATCCGAACCCACACC+CGG	+	Chr8:18654473-18654492	MsG0880043081.01.T01:CDS	65.0%
	ACCGGGTGTGGGTTCGGATC+CGG	-	Chr8:18654477-18654496	None:intergenic	65.0%
	GCATAGGGCGAGACAAGCGG+CGG	+	Chr8:18654838-18654857	MsG0880043081.01.T01:CDS	65.0%
	TCCGTCAGCCGCACCGCAAA+AGG	+	Chr8:18654398-18654417	MsG0880043081.01.T01:CDS	65.0%
!	CGGAGCAGCGTTGGAGGAGT+TGG	-	Chr8:18654382-18654401	None:intergenic	65.0%
	GCGGGAACCAGTTGGGGCAC+CGG	-	Chr8:18654495-18654514	None:intergenic	70.0%
	TGCGGCTGACGGAGCAGCGT+TGG	-	Chr8:18654391-18654410	None:intergenic	70.0%
!	CGGGAACCAGTTGGGGCACC+GGG	-	Chr8:18654494-18654513	None:intergenic	70.0%
!	GGCTGACGGAGCAGCGTTGG+AGG	-	Chr8:18654388-18654407	None:intergenic	70.0%
!!	ACCAGTTGGGGCACCGGGTG+TGG	-	Chr8:18654489-18654508	None:intergenic	70.0%
!!	CCAGTTGGGGCACCGGGTGT+GGG	-	Chr8:18654488-18654507	None:intergenic	70.0%
!!	TGGGGCACCGGGTGTGGGTT+CGG	-	Chr8:18654483-18654502	None:intergenic	70.0%
	CCCACACCCGGTGCCCCAAC+TGG	+	Chr8:18654485-18654504	MsG0880043081.01.T01:CDS	75.0%

Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	18654326	18655057	18654326	ID=MsG0880043081.01;Name=MsG0880043081.01
Chr8	mRNA	18654326	18655057	18654326	ID=MsG0880043081.01.T01;Parent=MsG0880043081.01;Name=MsG0880043081.01.T01;_AED=0.49;_eAED=0.49;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|243
Chr8	exon	18654326	18655057	18654326	ID=MsG0880043081.01.T01:exon:4900;Parent=MsG0880043081.01.T01
Chr8	CDS	18654326	18655057	18654326	ID=MsG0880043081.01.T01:cds;Parent=MsG0880043081.01.T01

Gene Sequence

>MsG0880043081.01.T01

ATGCTTCGCCTCACAAAGCTAAAACCACCATCACCACTGTCATCCTCCCCACACCAACTCCTCCAACGCTGCTCCGTCAGCCGCACCGCAAAAGGAAAAGGGAAAGCCGGTCAAATCCTAAAACGTTCCAAAATCACTGTCAAAAAAACCGGATCCGAACCCACACCCGGTGCCCCAACTGGTTCCCGCGAGAAACAAGAACGCGAACGCCTCTACGATCAATGCCTCAACGCACCAACACCACTTCGTTTTCTCAAACCCAAACAACGTGCACGCGAAGCTGAACGAGAAAAATTAGGGTTAATCAGCAAAGATCGTCAAATTGAACTCGATATGATGAAGAAGAAAAACGCGAAGTTTAGGGTTTCTGAGAAGCCAACGATAATGGGAACACCTGGGTTGGATTATATAACATTAGGGTTAGTGGATGCTGAGAAATTGCCGAAATATGAATTGACGAAAGAAGATGGGATGAAATTGGCCAAGGAGTATAGTAGGGTTTTGATGAGGAAGCATAGGGCGAGACAAGCGGCGGAGACGAATCTTTTGAGGATGAAAAAGGAGGCAATTGAAGCTTTACCTGGAGGTTTGAAGGAAGCTGCTTTGGTGCCGGATTTGACTCCGTTTCCGGTGAATAGGTTTATGGCGACGCTTACGCCACCTATTGATGGGTATATTGAGAAGATTAAGGAGGCTGCTGATAGGATCAGTGGGAAGGAGAAGATTAGGTAG

Protein sequence

>MsG0880043081.01.T01

MLRLTKLKPPSPLSSSPHQLLQRCSVSRTAKGKGKAGQILKRSKITVKKTGSEPTPGAPTGSREKQERERLYDQCLNAPTPLRFLKPKQRAREAEREKLGLISKDRQIELDMMKKKNAKFRVSEKPTIMGTPGLDYITLGLVDAEKLPKYELTKEDGMKLAKEYSRVLMRKHRARQAAETNLLRMKKEAIEALPGGLKEAALVPDLTPFPVNRFMATLTPPIDGYIEKIKEAADRISGKEKIR*