AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0080048784.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048784.01.T01 MTR_5g007240 100.000 117 0 0 1 117 50 166 3.47e-81 235
MsG0080048784.01.T01 MTR_5g096490 100.000 117 0 0 1 117 50 166 3.47e-81 235
MsG0080048784.01.T01 MTR_5g096490 100.000 117 0 0 1 117 17 133 3.82e-81 234
MsG0080048784.01.T01 MTR_4g051390 42.735 117 65 1 2 116 5 121 4.80e-31 107
MsG0080048784.01.T01 MTR_1779s0020 42.735 117 65 1 2 116 5 121 1.04e-29 103
MsG0080048784.01.T01 MTR_2g087550 41.026 117 67 1 2 116 5 121 6.95e-28 101
MsG0080048784.01.T01 MTR_0002s0780 41.739 115 64 1 5 116 135 249 7.70e-25 94.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048784.01.T01 AT5G46160 78.226 124 20 1 1 117 49 172 4.99e-62 187
MsG0080048784.01.T01 AT5G46160 78.226 124 20 1 1 117 50 173 5.69e-62 187
MsG0080048784.01.T01 AT1G17560 59.310 145 30 3 1 116 51 195 1.11e-48 154
MsG0080048784.01.T01 AT1G17560 59.310 145 30 3 1 116 28 172 1.65e-48 153
MsG0080048784.01.T01 ATCG00780 44.444 117 63 1 2 116 5 121 1.43e-32 110

Find 33 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGAAGGTTGTGGACAATTC+TGG 0.240087 contig440end:-8431 MsG0080048784.01.T01:CDS
GGACAATTCTGGAGCTAAAA+AGG 0.321429 contig440end:-8420 MsG0080048784.01.T01:CDS
GACAAACAAGGGCAGCCTAT+TGG 0.362318 contig440end:-8197 MsG0080048784.01.T01:CDS
ACAAACAAGGGCAGCCTATT+GGG 0.366691 contig440end:-8196 MsG0080048784.01.T01:CDS
GTCAAGATCCTTACCTTAGC+TGG 0.374088 contig440end:-8125 MsG0080048784.01.T01:CDS
GTGAAGGAAGCACATCCTAA+TGG 0.397241 contig440end:-8332 MsG0080048784.01.T01:CDS
TGCCTCAACTCATGAGGAAC+TGG 0.420523 contig440end:+8158 None:intergenic
CCGAAAACTCTGGTCCCAAT+AGG 0.444490 contig440end:+8182 None:intergenic
CGCGCGTTGGCAGGGAAGAA+AGG 0.457182 contig440end:-8386 MsG0080048784.01.T01:CDS
GGGAAGAAAGGAGCAAGATT+AGG 0.462937 contig440end:-8374 MsG0080048784.01.T01:CDS
ATGTGCATACGCGCGTTGGC+AGG 0.463329 contig440end:-8395 MsG0080048784.01.T01:CDS
TCCTAATGGAAAAGTGAAGA+AGG 0.493277 contig440end:-8318 MsG0080048784.01.T01:CDS
CTGCCATCACACCTACCCTT+TGG 0.494271 contig440end:+8257 None:intergenic
ATGCCAAAGGGTAGGTGTGA+TGG 0.533908 contig440end:-8260 MsG0080048784.01.T01:CDS
CAATATGCCCAGCTAAGGTA+AGG 0.539065 contig440end:+8117 None:intergenic
CATTGCTGCACGAACAACTA+CGG 0.550383 contig440end:+8280 None:intergenic
AGGAACTGGTCCGAAAACTC+TGG 0.550755 contig440end:+8172 None:intergenic
TCAAGATCCTTACCTTAGCT+GGG 0.562147 contig440end:-8124 MsG0080048784.01.T01:CDS
CTATGCAATATGCCCAGCTA+AGG 0.565511 contig440end:+8112 None:intergenic
TGTTCGTGCAGCAATGCCAA+AGG 0.568662 contig440end:-8273 MsG0080048784.01.T01:CDS
TGTGCATACGCGCGTTGGCA+GGG 0.580036 contig440end:-8394 MsG0080048784.01.T01:CDS
GACCAGTTCCTCATGAGTTG+AGG 0.580661 contig440end:-8160 MsG0080048784.01.T01:CDS
ACAGATGAGGACTGTTCTGA+AGG 0.580846 contig440end:-8447 None:intergenic
GAGGACTGTTCTGAAGGTTG+TGG 0.589490 contig440end:-8441 MsG0080048784.01.T01:CDS
GGTGATGTGCATACGCGCGT+TGG 0.592616 contig440end:-8399 MsG0080048784.01.T01:CDS
GTTCGTGCAGCAATGCCAAA+GGG 0.599925 contig440end:-8272 MsG0080048784.01.T01:CDS
CCTAATGGAAAAGTGAAGAA+GGG 0.626002 contig440end:-8317 MsG0080048784.01.T01:CDS
TTCTTCTGCCTCAACTCATG+AGG 0.632900 contig440end:+8152 None:intergenic
GTGCAGCAATGCCAAAGGGT+AGG 0.641762 contig440end:-8268 MsG0080048784.01.T01:CDS
GTTGTGCTTGTTGACAAACA+AGG 0.656177 contig440end:-8209 MsG0080048784.01.T01:CDS
TATGATACAAGCATCAGTGA+AGG 0.662607 contig440end:-8348 MsG0080048784.01.T01:CDS
TTGTGCTTGTTGACAAACAA+GGG 0.669343 contig440end:-8208 MsG0080048784.01.T01:CDS
GGGTAGGTGTGATGGCAGTG+AGG 0.701393 contig440end:-8252 MsG0080048784.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
CCTAATGGAAAAGTGAAGAA+GGG - contig440end:8238-8257 MsG0080048784.01.T01:CDS 35.0%
TATGATACAAGCATCAGTGA+AGG - contig440end:8207-8226 MsG0080048784.01.T01:CDS 35.0%
TCCTAATGGAAAAGTGAAGA+AGG - contig440end:8237-8256 MsG0080048784.01.T01:CDS 35.0%
! TTGTGCTTGTTGACAAACAA+GGG - contig440end:8347-8366 MsG0080048784.01.T01:CDS 35.0%
TCAAGATCCTTACCTTAGCT+GGG - contig440end:8431-8450 MsG0080048784.01.T01:CDS 40.0%
! GTTGTGCTTGTTGACAAACA+AGG - contig440end:8346-8365 MsG0080048784.01.T01:CDS 40.0%
!! CCCTTCTTCACTTTTCCATT+AGG + contig440end:8241-8260 None:intergenic 40.0%
!! GGACAATTCTGGAGCTAAAA+AGG - contig440end:8135-8154 MsG0080048784.01.T01:CDS 40.0%
ACAAACAAGGGCAGCCTATT+GGG - contig440end:8359-8378 MsG0080048784.01.T01:CDS 45.0%
CAATATGCCCAGCTAAGGTA+AGG + contig440end:8441-8460 None:intergenic 45.0%
CATTGCTGCACGAACAACTA+CGG + contig440end:8278-8297 None:intergenic 45.0%
CTATGCAATATGCCCAGCTA+AGG + contig440end:8446-8465 None:intergenic 45.0%
CTGAAGGTTGTGGACAATTC+TGG - contig440end:8124-8143 MsG0080048784.01.T01:CDS 45.0%
GGGAAGAAAGGAGCAAGATT+AGG - contig440end:8181-8200 MsG0080048784.01.T01:CDS 45.0%
GTCAAGATCCTTACCTTAGC+TGG - contig440end:8430-8449 MsG0080048784.01.T01:CDS 45.0%
TTCTTCTGCCTCAACTCATG+AGG + contig440end:8406-8425 None:intergenic 45.0%
! CCTATTGGGACCAGAGTTTT+CGG - contig440end:8373-8392 MsG0080048784.01.T01:CDS 45.0%
! GTGAAGGAAGCACATCCTAA+TGG - contig440end:8223-8242 MsG0080048784.01.T01:CDS 45.0%
AGGAACTGGTCCGAAAACTC+TGG + contig440end:8386-8405 None:intergenic 50.0%
ATGCCAAAGGGTAGGTGTGA+TGG - contig440end:8295-8314 MsG0080048784.01.T01:CDS 50.0%
CCGAAAACTCTGGTCCCAAT+AGG + contig440end:8376-8395 None:intergenic 50.0%
GACAAACAAGGGCAGCCTAT+TGG - contig440end:8358-8377 MsG0080048784.01.T01:CDS 50.0%
GACCAGTTCCTCATGAGTTG+AGG - contig440end:8395-8414 MsG0080048784.01.T01:CDS 50.0%
GTTCGTGCAGCAATGCCAAA+GGG - contig440end:8283-8302 MsG0080048784.01.T01:CDS 50.0%
TGCCTCAACTCATGAGGAAC+TGG + contig440end:8400-8419 None:intergenic 50.0%
TGTTCGTGCAGCAATGCCAA+AGG - contig440end:8282-8301 MsG0080048784.01.T01:CDS 50.0%
!! GAGGACTGTTCTGAAGGTTG+TGG - contig440end:8114-8133 MsG0080048784.01.T01:CDS 50.0%
CTGCCATCACACCTACCCTT+TGG + contig440end:8301-8320 None:intergenic 55.0%
GTGCAGCAATGCCAAAGGGT+AGG - contig440end:8287-8306 MsG0080048784.01.T01:CDS 55.0%
GGGTAGGTGTGATGGCAGTG+AGG - contig440end:8303-8322 MsG0080048784.01.T01:CDS 60.0%
GGTGATGTGCATACGCGCGT+TGG - contig440end:8156-8175 MsG0080048784.01.T01:CDS 60.0%
! ATGTGCATACGCGCGTTGGC+AGG - contig440end:8160-8179 MsG0080048784.01.T01:CDS 60.0%
! TGTGCATACGCGCGTTGGCA+GGG - contig440end:8161-8180 MsG0080048784.01.T01:CDS 60.0%
! CGCGCGTTGGCAGGGAAGAA+AGG - contig440end:8169-8188 MsG0080048784.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
contig440end gene 8112 8465 8112 ID=MsG0080048784.01;Name=MsG0080048784.01
contig440end mRNA 8112 8465 8112 ID=MsG0080048784.01.T01;Parent=MsG0080048784.01;Name=MsG0080048784.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|117
contig440end exon 8112 8465 8112 ID=MsG0080048784.01.T01:exon:1031;Parent=MsG0080048784.01.T01
contig440end CDS 8112 8465 8112 ID=MsG0080048784.01.T01:cds;Parent=MsG0080048784.01.T01
Gene Sequence

>MsG0080048784.01.T01

ATGAGGACTGTTCTGAAGGTTGTGGACAATTCTGGAGCTAAAAAGGTGATGTGCATACGCGCGTTGGCAGGGAAGAAAGGAGCAAGATTAGGTGATATGATACAAGCATCAGTGAAGGAAGCACATCCTAATGGAAAAGTGAAGAAGGGAGCTGTTGTATTTGCCGTAGTTGTTCGTGCAGCAATGCCAAAGGGTAGGTGTGATGGCAGTGAGGTCAAGTTTGACGACAATGCTGTTGTGCTTGTTGACAAACAAGGGCAGCCTATTGGGACCAGAGTTTTCGGACCAGTTCCTCATGAGTTGAGGCAGAAGAAGCATGTCAAGATCCTTACCTTAGCTGGGCATATTGCATAG

Protein sequence

>MsG0080048784.01.T01

MRTVLKVVDNSGAKKVMCIRALAGKKGARLGDMIQASVKEAHPNGKVKKGAVVFAVVVRAAMPKGRCDGSEVKFDDNAVVLVDKQGQPIGTRVFGPVPHELRQKKHVKILTLAGHIA*