AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880042324.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880042324.01.T01 MTR_8g013650 91.964 224 16 1 1 224 13 234 2.73e-115 329
MsG0880042324.01.T01 MTR_7g056407 48.673 113 47 2 112 224 238 339 2.22e-28 109
MsG0880042324.01.T01 MTR_4g132760 50.420 119 52 3 111 224 101 217 6.00e-28 105
MsG0880042324.01.T01 MTR_3g065180 47.321 112 51 2 113 224 237 340 1.15e-24 99.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880042324.01.T01 AT3G25030 35.193 233 102 7 1 224 18 210 2.00e-33 120
MsG0880042324.01.T01 AT3G25030 32.453 265 106 7 1 224 18 250 4.37e-31 115
MsG0880042324.01.T01 AT3G25030 32.453 265 106 7 1 224 18 250 4.37e-31 115
MsG0880042324.01.T01 AT3G25030 32.453 265 106 7 1 224 18 250 4.37e-31 115
MsG0880042324.01.T01 AT4G13100 51.613 124 39 4 106 224 149 256 1.09e-30 114
MsG0880042324.01.T01 AT4G13100 51.613 124 39 4 106 224 149 256 1.09e-30 114
MsG0880042324.01.T01 AT4G13100 51.613 124 39 4 106 224 158 265 1.24e-30 114
MsG0880042324.01.T01 AT4G13100 55.357 112 29 4 118 224 161 256 1.64e-30 113
MsG0880042324.01.T01 AT4G13100 55.357 112 29 4 118 224 164 259 1.72e-30 113
MsG0880042324.01.T01 AT4G13100 55.357 112 29 4 118 224 164 259 1.72e-30 113
MsG0880042324.01.T01 AT4G13100 55.357 112 29 4 118 224 164 259 1.72e-30 113
MsG0880042324.01.T01 AT4G13100 55.357 112 29 4 118 224 164 259 1.72e-30 113
MsG0880042324.01.T01 AT4G22250 56.790 81 35 0 144 224 134 214 5.05e-27 103
MsG0880042324.01.T01 AT4G03965 48.333 120 38 4 108 224 111 209 8.48e-27 102
MsG0880042324.01.T01 AT1G62370 71.429 49 14 0 176 224 156 204 1.21e-23 94.4
MsG0880042324.01.T01 AT3G07120 45.370 108 54 1 117 224 258 360 9.24e-23 95.1

Find 37 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGATAAAACGGCATCGTTT+TGG 0.314261 8:-7119265 None:intergenic
GATCCGTCGGAATTTCTTCC+CGG 0.352946 8:+7119515 MsG0880042324.01.T01:CDS
GGAAGAGAGTGAAATTGAAT+TGG 0.374216 8:+7119592 MsG0880042324.01.T01:CDS
ACATTAACAACAACGTTGTT+TGG 0.375493 8:-7119338 None:intergenic
GAATGTGTTCAAGAGAGCTT+TGG 0.390646 8:+7119810 MsG0880042324.01.T01:CDS
GCTGTTATTGTTGTTGTTGT+CGG 0.397472 8:-7119369 None:intergenic
GTATAATATCTAAGAGTGTT+TGG 0.411313 8:-7119304 None:intergenic
CATGTCGTTGATGGATTTGT+TGG 0.421193 8:+7119571 MsG0880042324.01.T01:CDS
GTGTGTATGGTGAGGGATAA+AGG 0.427186 8:+7119752 MsG0880042324.01.T01:CDS
AGGAGAAGGGAGTAATGTTA+AGG 0.433410 8:+7119712 MsG0880042324.01.T01:CDS
GAAGAAGAAAGAGAAGAAGA+AGG 0.437441 8:+7119692 MsG0880042324.01.T01:CDS
AAAATCATGGAAAGCTTTCA+AGG 0.440126 8:+7119412 MsG0880042324.01.T01:CDS
AATGTGTTCAAGAGAGCTTT+GGG 0.444478 8:+7119811 MsG0880042324.01.T01:CDS
AAAGAGAAGAAGAAGGAGAA+GGG 0.457534 8:+7119699 MsG0880042324.01.T01:CDS
GAAAGAGAAGAAGAAGGAGA+AGG 0.463737 8:+7119698 MsG0880042324.01.T01:CDS
GCAGGTTAGCATGTCGTTGA+TGG 0.473676 8:+7119562 MsG0880042324.01.T01:CDS
ACACAATTGTTGCGTGTGTA+TGG 0.501642 8:+7119739 MsG0880042324.01.T01:CDS
AGTAGTGATGTTGGTGATGA+TGG 0.514735 8:+7119620 MsG0880042324.01.T01:CDS
TTGTTAAAGATCGAAAATCA+TGG 0.528304 8:+7119399 MsG0880042324.01.T01:CDS
GCTAACCTGCGCCGTCTCAT+CGG 0.529102 8:-7119549 None:intergenic
AGAGAGCTTTGGGTTAGTAG+AGG 0.548126 8:+7119821 MsG0880042324.01.T01:CDS
TCATCGGAAAACTCGCCGCC+GGG 0.552591 8:-7119533 None:intergenic
AGAAGGGAGTAATGTTAAGG+TGG 0.555525 8:+7119715 MsG0880042324.01.T01:CDS
CTCATCGGAAAACTCGCCGC+CGG 0.561315 8:-7119534 None:intergenic
CTGCAAAACGTGTGTCCACA+TGG 0.562500 8:-7119788 None:intergenic
TCCGACGGATCTGAATTTGT+CGG 0.582062 8:-7119503 None:intergenic
TTGGAACGAAGTAGTGATGT+TGG 0.593831 8:+7119611 MsG0880042324.01.T01:CDS
TCCGACAAATTCAGATCCGT+CGG 0.594548 8:+7119502 MsG0880042324.01.T01:CDS
AAAATAAAATGATTGCAAAG+AGG 0.605793 8:-7119851 None:intergenic
TGAACTTCTAGTGTTGCTTG+TGG 0.612649 8:-7119228 None:intergenic
TGGTGATGATGGTGATGACG+TGG 0.654289 8:+7119631 MsG0880042324.01.T01:CDS
CCGCCGGGAAGAAATTCCGA+CGG 0.660224 8:-7119518 None:intergenic
TTGTTGCGTGTGTATGGTGA+GGG 0.665548 8:+7119745 MsG0880042324.01.T01:CDS
CCGTCGGAATTTCTTCCCGG+CGG 0.683308 8:+7119518 MsG0880042324.01.T01:CDS
ATTGTTGCGTGTGTATGGTG+AGG 0.686096 8:+7119744 MsG0880042324.01.T01:CDS
GGTGCAGCGTTTATACCATG+TGG 0.688981 8:+7119773 MsG0880042324.01.T01:CDS
TCACCAACAACAACAAAACG+AGG 0.705279 8:+7119475 MsG0880042324.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAAAATGATTGCAAAG+AGG - Chr8:7119854-7119873 None:intergenic 20.0%
! TTGTTAAAGATCGAAAATCA+TGG + Chr8:7119399-7119418 MsG0880042324.01.T01:CDS 25.0%
!!! GTATAATATCTAAGAGTGTT+TGG - Chr8:7119307-7119326 None:intergenic 25.0%
!!! GTTTTTTTGGTTGGATAAAA+CGG - Chr8:7119279-7119298 None:intergenic 25.0%
AAAATCATGGAAAGCTTTCA+AGG + Chr8:7119412-7119431 MsG0880042324.01.T01:CDS 30.0%
ACATTAACAACAACGTTGTT+TGG - Chr8:7119341-7119360 None:intergenic 30.0%
!!! GTGTTTGGTTTGTGTTTTTT+TGG - Chr8:7119292-7119311 None:intergenic 30.0%
!!! TTGGTTTGTGTTTTTTTGGT+TGG - Chr8:7119288-7119307 None:intergenic 30.0%
AAAGAGAAGAAGAAGGAGAA+GGG + Chr8:7119699-7119718 MsG0880042324.01.T01:CDS 35.0%
AATGTGTTCAAGAGAGCTTT+GGG + Chr8:7119811-7119830 MsG0880042324.01.T01:CDS 35.0%
GAAGAAGAAAGAGAAGAAGA+AGG + Chr8:7119692-7119711 MsG0880042324.01.T01:CDS 35.0%
GGAAGAGAGTGAAATTGAAT+TGG + Chr8:7119592-7119611 MsG0880042324.01.T01:CDS 35.0%
! GCTGTTATTGTTGTTGTTGT+CGG - Chr8:7119372-7119391 None:intergenic 35.0%
!!! TATCCTCGTTTTGTTGTTGT+TGG - Chr8:7119481-7119500 None:intergenic 35.0%
ACACAATTGTTGCGTGTGTA+TGG + Chr8:7119739-7119758 MsG0880042324.01.T01:CDS 40.0%
AGAAGGGAGTAATGTTAAGG+TGG + Chr8:7119715-7119734 MsG0880042324.01.T01:CDS 40.0%
AGGAGAAGGGAGTAATGTTA+AGG + Chr8:7119712-7119731 MsG0880042324.01.T01:CDS 40.0%
GAAAGAGAAGAAGAAGGAGA+AGG + Chr8:7119698-7119717 MsG0880042324.01.T01:CDS 40.0%
GAATGTGTTCAAGAGAGCTT+TGG + Chr8:7119810-7119829 MsG0880042324.01.T01:CDS 40.0%
TCACCAACAACAACAAAACG+AGG + Chr8:7119475-7119494 MsG0880042324.01.T01:CDS 40.0%
TTGGAACGAAGTAGTGATGT+TGG + Chr8:7119611-7119630 MsG0880042324.01.T01:CDS 40.0%
! TGGATAAAACGGCATCGTTT+TGG - Chr8:7119268-7119287 None:intergenic 40.0%
!! AGTAGTGATGTTGGTGATGA+TGG + Chr8:7119620-7119639 MsG0880042324.01.T01:CDS 40.0%
!! CATGTCGTTGATGGATTTGT+TGG + Chr8:7119571-7119590 MsG0880042324.01.T01:CDS 40.0%
!! TGAACTTCTAGTGTTGCTTG+TGG - Chr8:7119231-7119250 None:intergenic 40.0%
ATTGTTGCGTGTGTATGGTG+AGG + Chr8:7119744-7119763 MsG0880042324.01.T01:CDS 45.0%
GTGTGTATGGTGAGGGATAA+AGG + Chr8:7119752-7119771 MsG0880042324.01.T01:CDS 45.0%
TCCGACAAATTCAGATCCGT+CGG + Chr8:7119502-7119521 MsG0880042324.01.T01:CDS 45.0%
TCCGACGGATCTGAATTTGT+CGG - Chr8:7119506-7119525 None:intergenic 45.0%
TTGTTGCGTGTGTATGGTGA+GGG + Chr8:7119745-7119764 MsG0880042324.01.T01:CDS 45.0%
!! AGAGAGCTTTGGGTTAGTAG+AGG + Chr8:7119821-7119840 MsG0880042324.01.T01:CDS 45.0%
CTGCAAAACGTGTGTCCACA+TGG - Chr8:7119791-7119810 None:intergenic 50.0%
GATCCGTCGGAATTTCTTCC+CGG + Chr8:7119515-7119534 MsG0880042324.01.T01:CDS 50.0%
GCAGGTTAGCATGTCGTTGA+TGG + Chr8:7119562-7119581 MsG0880042324.01.T01:CDS 50.0%
GGTGCAGCGTTTATACCATG+TGG + Chr8:7119773-7119792 MsG0880042324.01.T01:CDS 50.0%
!! TGGTGATGATGGTGATGACG+TGG + Chr8:7119631-7119650 MsG0880042324.01.T01:CDS 50.0%
! CGGCGAGTTTTCCGATGAGA+CGG + Chr8:7119538-7119557 MsG0880042324.01.T01:CDS 55.0%
CCGCCGGGAAGAAATTCCGA+CGG - Chr8:7119521-7119540 None:intergenic 60.0%
CCGTCGGAATTTCTTCCCGG+CGG + Chr8:7119518-7119537 MsG0880042324.01.T01:CDS 60.0%
CTCATCGGAAAACTCGCCGC+CGG - Chr8:7119537-7119556 None:intergenic 60.0%
GCTAACCTGCGCCGTCTCAT+CGG - Chr8:7119552-7119571 None:intergenic 60.0%
TCATCGGAAAACTCGCCGCC+GGG - Chr8:7119536-7119555 None:intergenic 60.0%
! GTTTTCCGATGAGACGGCGC+AGG + Chr8:7119544-7119563 MsG0880042324.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr8 gene 7119221 7119895 7119221 ID=MsG0880042324.01;Name=MsG0880042324.01
Chr8 mRNA 7119221 7119895 7119221 ID=MsG0880042324.01.T01;Parent=MsG0880042324.01;Name=MsG0880042324.01.T01;_AED=0.33;_eAED=0.33;_QI=0|-1|0|1|-1|1|1|0|224
Chr8 exon 7119221 7119895 7119221 ID=MsG0880042324.01.T01:exon:3173;Parent=MsG0880042324.01.T01
Chr8 CDS 7119221 7119895 7119221 ID=MsG0880042324.01.T01:cds;Parent=MsG0880042324.01.T01
Gene Sequence

>MsG0880042324.01.T01

ATGACGTCCACAAGCAACACTAGAAGTTCACTAGCTAGTTTAATCCAAAACGATGCCGTTTTATCCAACCAAAAAAACACAAACCAAACACTCTTAGATATTATACGAGAAAACGAACCAAACAACGTTGTTGTTAATGTTAGTGTTACCGACAACAACAACAATAACAGCAACAACGTTGTTAAAGATCGAAAATCATGGAAAGCTTTCAAGGAATTGCTTCTTTTCAAACGTCGTAACTCTGAAGAATCAACTCACCAACAACAACAAAACGAGGATATTCCGACAAATTCAGATCCGTCGGAATTTCTTCCCGGCGGCGAGTTTTCCGATGAGACGGCGCAGGTTAGCATGTCGTTGATGGATTTGTTGGAAGAGAGTGAAATTGAATTGGAACGAAGTAGTGATGTTGGTGATGATGGTGATGACGTGGAAGAGAATAATGAGAAACGAGAAGAAGAAGAAAAAGAAGAAGAAGAAAGAGAAGAAGAAGGAGAAGGGAGTAATGTTAAGGTGGAACACAATTGTTGCGTGTGTATGGTGAGGGATAAAGGTGCAGCGTTTATACCATGTGGACACACGTTTTGCAGAATGTGTTCAAGAGAGCTTTGGGTTAGTAGAGGAAATTGTCCTCTTTGCAATCATTTTATTTTAGAAGTTCTTGATATTTTCTGA

Protein sequence

>MsG0880042324.01.T01

MTSTSNTRSSLASLIQNDAVLSNQKNTNQTLLDIIRENEPNNVVVNVSVTDNNNNNSNNVVKDRKSWKAFKELLLFKRRNSEESTHQQQQNEDIPTNSDPSEFLPGGEFSDETAQVSMSLMDLLEESEIELERSSDVGDDGDDVEENNEKREEEEKEEEEREEEGEGSNVKVEHNCCVCMVRDKGAAFIPCGHTFCRMCSRELWVSRGNCPLCNHFILEVLDIF*