AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680032899.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680032899.01.T01 MTR_6g049280 93.846 130 8 0 42 171 25 154 1.32e-78 231
MsG0680032899.01.T01 MTR_6g049320 93.077 130 9 0 42 171 25 154 4.62e-76 224
MsG0680032899.01.T01 MTR_6g049280 92.742 124 9 0 48 171 1 124 2.59e-72 214
MsG0680032899.01.T01 MTR_6g049320 92.742 124 9 0 48 171 1 124 3.51e-71 211
MsG0680032899.01.T01 MTR_6g051690 59.690 129 45 3 48 171 1 127 2.88e-37 125
MsG0680032899.01.T01 MTR_7g451200 55.556 108 46 1 48 155 1 106 1.36e-36 122
MsG0680032899.01.T01 MTR_1g099800 55.556 135 48 2 48 170 1 135 1.13e-35 121
MsG0680032899.01.T01 MTR_1173s0010 55.140 107 46 1 48 154 1 105 2.16e-35 119
MsG0680032899.01.T01 MTR_7g451210 56.075 107 45 1 48 154 1 105 1.07e-34 118
MsG0680032899.01.T01 MTR_6g049260 57.944 107 43 2 48 154 1 105 2.40e-34 117
MsG0680032899.01.T01 MTR_3g434890 55.140 107 46 1 48 154 1 105 3.36e-34 117
MsG0680032899.01.T01 MTR_3g434960 54.206 107 47 1 48 154 1 105 1.09e-33 115
MsG0680032899.01.T01 MTR_1g099815 51.111 135 50 4 48 170 1 131 1.43e-33 115
MsG0680032899.01.T01 MTR_1g099810 51.799 139 50 3 48 170 1 138 1.44e-33 116
MsG0680032899.01.T01 MTR_6g051680 75.714 70 17 0 48 117 1 70 2.82e-33 114
MsG0680032899.01.T01 MTR_8g098915 48.855 131 56 3 48 171 1 127 5.79e-32 111
MsG0680032899.01.T01 MTR_1g099640 47.761 134 56 2 48 171 1 130 2.65e-31 110
MsG0680032899.01.T01 MTR_3g435000 51.376 109 50 2 45 153 3 108 5.34e-31 108
MsG0680032899.01.T01 MTR_1g102240 51.111 135 50 4 48 170 1 131 3.01e-30 107
MsG0680032899.01.T01 MTR_0795s0020 52.239 134 47 3 48 171 1 127 1.23e-28 103
MsG0680032899.01.T01 MTR_1g099810 51.667 120 41 3 67 170 1 119 5.32e-25 93.6
MsG0680032899.01.T01 MTR_7g079110 60.294 68 27 0 48 115 1 68 3.50e-23 90.5
MsG0680032899.01.T01 MTR_8g098915 46.429 112 49 3 67 171 1 108 1.18e-21 84.7
MsG0680032899.01.T01 MTR_3g434920 53.750 80 35 1 75 154 2 79 1.51e-21 84.0
MsG0680032899.01.T01 MTR_1g099810 46.154 104 39 3 83 170 1 103 1.46e-14 66.2
MsG0680032899.01.T01 MTR_8g028615 64.706 68 24 0 48 115 1 68 3.60e-14 66.6
MsG0680032899.01.T01 MTR_1g099640 42.424 99 43 2 83 171 1 95 4.82e-14 64.7
MsG0680032899.01.T01 MTR_3g082250 43.902 82 42 4 49 126 4 85 1.61e-13 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680032899.01.T01 AT1G01490 64.789 71 24 1 48 117 1 71 2.64e-24 93.6
MsG0680032899.01.T01 AT1G01490 64.789 71 24 1 48 117 1 71 2.64e-24 93.6
MsG0680032899.01.T01 AT1G01490 66.667 69 22 1 50 117 30 98 7.76e-24 93.2
MsG0680032899.01.T01 AT5G52740 57.971 69 27 1 48 116 1 67 1.17e-18 77.4
MsG0680032899.01.T01 AT5G52760 41.121 107 53 4 49 152 4 103 1.50e-15 69.7
MsG0680032899.01.T01 AT5G52750 44.615 65 36 0 50 114 5 69 9.77e-12 59.7
MsG0680032899.01.T01 AT1G63960 35.065 77 49 1 41 116 4 80 1.75e-11 59.3
MsG0680032899.01.T01 AT5G23760 50.000 72 35 1 47 117 1 72 6.16e-11 57.0

Find 33 sgRNAs with CRISPR-Local

Find 148 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGACTGTCTCAGGCCTTTC+TGG 0.185291 6:-52396382 MsG0680032899.01.T01:intron
GACAAGAAATTAACCTTAAC+TGG 0.272704 6:-52395721 MsG0680032899.01.T01:CDS
ACTAATTGATATTGAACAAT+TGG 0.374516 6:-52398821 MsG0680032899.01.T01:intron
ATTTGATTCTTCTTCACATC+AGG 0.383077 6:+52395592 None:intergenic
ATATTCCTGTGTTTCAAGAA+TGG 0.407778 6:-52395512 MsG0680032899.01.T01:CDS
TGGATCTGTGTCTCCAGTTA+AGG 0.422934 6:+52395708 None:intergenic
TTCCTGCATCATAATAGATC+CGG 0.432826 6:+52398843 None:intergenic
GTTACAGAAACTAGTATTGA+AGG 0.451235 6:-52396443 MsG0680032899.01.T01:CDS
AGTTTGCTTACTACATGTAC+TGG 0.452527 6:+52395688 None:intergenic
TAGTAAGCAAACTAAGGAAA+TGG 0.458647 6:-52395678 MsG0680032899.01.T01:CDS
ATGCTGAAATAGTTTCTGTT+GGG 0.476513 6:-52395651 MsG0680032899.01.T01:CDS
ATCAGAAGTTTACAAATCGT+TGG 0.486506 6:-52398885 MsG0680032899.01.T01:CDS
TTACAAATCGTTGGCAAGAC+CGG 0.487943 6:-52398876 MsG0680032899.01.T01:CDS
AAAGCTATGAAGACTGTCTC+AGG 0.497682 6:-52396391 MsG0680032899.01.T01:CDS
CATGCTGAAATAGTTTCTGT+TGG 0.500770 6:-52395652 MsG0680032899.01.T01:CDS
TCCCGGATCTATTATGATGC+AGG 0.501273 6:-52398845 MsG0680032899.01.T01:CDS
AATCGTTGGCAAGACCGGTT+GGG 0.506962 6:-52398871 MsG0680032899.01.T01:CDS
CAAGACCGGTTGGGTTATCC+CGG 0.508770 6:-52398862 MsG0680032899.01.T01:CDS
TACATGTAGTAAGCAAACTA+AGG 0.515410 6:-52395684 MsG0680032899.01.T01:CDS
CAGCATATGACACATCCAAC+AGG 0.518380 6:+52395481 None:intergenic
ACAATGAGCTTGTAACATGT+TGG 0.535420 6:-52395367 MsG0680032899.01.T01:three_prime_UTR
AAATCGTTGGCAAGACCGGT+TGG 0.540431 6:-52398872 MsG0680032899.01.T01:CDS
TTGTTCCATTCTTGAAACAC+AGG 0.547137 6:+52395507 None:intergenic
TCCTGCATCATAATAGATCC+GGG 0.551671 6:+52398844 None:intergenic
TAGATCCGGGATAACCCAAC+CGG 0.557503 6:+52398857 None:intergenic
CAGGAATATTGAATATATTG+TGG 0.566124 6:+52395526 None:intergenic
TGTTCAATTGTGCAAACAGA+GGG 0.585753 6:-52395317 MsG0680032899.01.T01:three_prime_UTR
ACAAGTTGACATTACCAGAA+AGG 0.591138 6:+52396368 None:intergenic
CATGTAGTAATAATGAGGAT+AGG 0.591628 6:+52395555 None:intergenic
AGAATGGAACAAGATCCTGT+TGG 0.628668 6:-52395496 MsG0680032899.01.T01:CDS
TGTTGCATGTAGTAATAATG+AGG 0.650568 6:+52395550 None:intergenic
ACAGAAACTAGTATTGAAGG+TGG 0.658613 6:-52396440 MsG0680032899.01.T01:CDS
GTGTTCAATTGTGCAAACAG+AGG 0.663444 6:-52395318 MsG0680032899.01.T01:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTTAGCAAAAAAATTAA+AGG + Chr6:52398433-52398452 None:intergenic 10.0%
!!! ACTTTTTTTTTTTTACAAAT+TGG + Chr6:52398169-52398188 None:intergenic 10.0%
!!! CTTTTTTTTTTTTACAAATT+GGG + Chr6:52398168-52398187 None:intergenic 10.0%
!!! TATTTTATATATTATTTGCA+GGG - Chr6:52398454-52398473 MsG0680032899.01.T01:intron 10.0%
!!! TGTATTTTCATATTATTTAT+TGG - Chr6:52398790-52398809 MsG0680032899.01.T01:intron 10.0%
!!! TTCTAAAATTTCTTATATAA+AGG - Chr6:52397016-52397035 MsG0680032899.01.T01:intron 10.0%
!!! TTTTTGAAATAATTAAGTAT+TGG - Chr6:52398190-52398209 MsG0680032899.01.T01:intron 10.0%
!! AAATAATTTATTTGAACTTC+AGG + Chr6:52395925-52395944 None:intergenic 15.0%
!! ATATGTCTCTTAAAATTAAA+TGG + Chr6:52396706-52396725 None:intergenic 15.0%
!! TATTAATATTCATAACTTTC+AGG + Chr6:52396246-52396265 None:intergenic 15.0%
!! TCAACATAATTAATTGTAAA+TGG + Chr6:52395809-52395828 None:intergenic 15.0%
!!! AAAAAATTGATAGTAAAAGA+GGG + Chr6:52397726-52397745 None:intergenic 15.0%
!!! ATCAATATGCTTTATTAATT+TGG - Chr6:52397355-52397374 MsG0680032899.01.T01:intron 15.0%
!!! ATGATATTTAAATTCTTTTG+AGG + Chr6:52398074-52398093 None:intergenic 15.0%
!!! ATTTTATATATTATTTGCAG+GGG - Chr6:52398455-52398474 MsG0680032899.01.T01:intron 15.0%
!!! GTATTTTATATATTATTTGC+AGG - Chr6:52398453-52398472 MsG0680032899.01.T01:intron 15.0%
!!! TGATTTATATATTTTTTGAG+TGG - Chr6:52398825-52398844 MsG0680032899.01.T01:CDS 15.0%
!! AAAATTTAAAAGAAGAGCTA+GGG + Chr6:52397628-52397647 None:intergenic 20.0%
!! AAATCATAATCTTCTTGTAT+GGG + Chr6:52397264-52397283 None:intergenic 20.0%
!! AAATTGTGAACAATTTGAAT+TGG - Chr6:52397120-52397139 MsG0680032899.01.T01:intron 20.0%
!! AAGATGTGTTACAATTTATT+TGG - Chr6:52396818-52396837 MsG0680032899.01.T01:intron 20.0%
!! ACATGAATATTTATTTCGTT+TGG + Chr6:52396034-52396053 None:intergenic 20.0%
!! ACTAATTGATATTGAACAAT+TGG - Chr6:52395404-52395423 MsG0680032899.01.T01:three_prime_UTR 20.0%
!! GAAAAATCTTGAAAGAATAT+TGG - Chr6:52395760-52395779 MsG0680032899.01.T01:intron 20.0%
!! GAAATTAAACTCATAATTGT+CGG - Chr6:52398242-52398261 MsG0680032899.01.T01:intron 20.0%
!! GATATTTATCAATTCACTTT+AGG + Chr6:52396681-52396700 None:intergenic 20.0%
!! TAAAATTTAAAAGAAGAGCT+AGG + Chr6:52397629-52397648 None:intergenic 20.0%
!! TTTGAAGATGTGAAATTAAT+TGG - Chr6:52396503-52396522 MsG0680032899.01.T01:intron 20.0%
!!! AAAAGCTTTATTGAAAATGA+TGG - Chr6:52397677-52397696 MsG0680032899.01.T01:intron 20.0%
!!! ATGTCTTATAAACTCTATTT+TGG - Chr6:52395542-52395561 MsG0680032899.01.T01:CDS 20.0%
!!! CTTGTGATTCTAATTTTATA+AGG - Chr6:52397299-52397318 MsG0680032899.01.T01:intron 20.0%
!!! GAAAAAATTGATAGTAAAAG+AGG + Chr6:52397727-52397746 None:intergenic 20.0%
!!! TCATTTTCATATCCTTAAAA+TGG + Chr6:52396938-52396957 None:intergenic 20.0%
!!! TGCTAACATTTTCAAATAAT+CGG + Chr6:52397205-52397224 None:intergenic 20.0%
!!! TGTCTTATAAACTCTATTTT+GGG - Chr6:52395543-52395562 MsG0680032899.01.T01:CDS 20.0%
!!! TTTCAATAAAGCTTTTTGAT+AGG + Chr6:52397673-52397692 None:intergenic 20.0%
! AAAGTTCTTGAATTCACTTT+AGG + Chr6:52396749-52396768 None:intergenic 25.0%
! AACATGTTGGAAAAATGAAA+AGG - Chr6:52398871-52398890 MsG0680032899.01.T01:CDS 25.0%
! ATACTGTATAAACTCTAGTT+TGG - Chr6:52395509-52395528 MsG0680032899.01.T01:CDS 25.0%
! CAGGAATATTGAATATATTG+TGG + Chr6:52398702-52398721 None:intergenic 25.0%
! CGGAAACATTTAATATATCA+AGG - Chr6:52398295-52398314 MsG0680032899.01.T01:intron 25.0%
! CTAAAATATGGTAGATCAAT+CGG - Chr6:52396091-52396110 MsG0680032899.01.T01:intron 25.0%
! GAAATCATAATCTTCTTGTA+TGG + Chr6:52397265-52397284 None:intergenic 25.0%
! GATTCTTTGTATCTTCATAT+TGG + Chr6:52397450-52397469 None:intergenic 25.0%
! TAAAAGTTGTATCACTTTCT+TGG - Chr6:52397408-52397427 MsG0680032899.01.T01:intron 25.0%
! TGGATATGTTGTGTATATAT+AGG - Chr6:52395628-52395647 MsG0680032899.01.T01:CDS 25.0%
! TTGATAAAACAGACTTTGTT+TGG - Chr6:52396463-52396482 MsG0680032899.01.T01:CDS 25.0%
!! ACTTGTCTAGTTTCAAAATT+TGG - Chr6:52397874-52397893 MsG0680032899.01.T01:intron 25.0%
!! TATTCGAGGCTTTTTATATT+TGG + Chr6:52396119-52396138 None:intergenic 25.0%
!! TCTAGTTTCAAAATTTGGTT+TGG - Chr6:52397879-52397898 MsG0680032899.01.T01:intron 25.0%
!! TTTTTCTTAGTGCATTACAT+TGG + Chr6:52395746-52395765 None:intergenic 25.0%
!!! AGAAATTTTAGAATCACTTC+AGG + Chr6:52397009-52397028 None:intergenic 25.0%
!!! GGATTAGTTTTTCCAATATT+AGG - Chr6:52395870-52395889 MsG0680032899.01.T01:intron 25.0%
!!! TAAATTCTTTTGAGGTTACT+AGG + Chr6:52398066-52398085 None:intergenic 25.0%
!!! TTAGTTTTTCCAATATTAGG+CGG - Chr6:52395873-52395892 MsG0680032899.01.T01:intron 25.0%
AAAAAGCCTCGAATAAGAAA+AGG - Chr6:52396124-52396143 MsG0680032899.01.T01:intron 30.0%
AAGATATAACTCGTTTGCAA+AGG - Chr6:52395978-52395997 MsG0680032899.01.T01:intron 30.0%
AGAATCACAAGTTTCTTGTA+TGG + Chr6:52397290-52397309 None:intergenic 30.0%
ATATTCCTGTGTTTCAAGAA+TGG - Chr6:52398713-52398732 MsG0680032899.01.T01:intron 30.0%
ATCAGAAGTTTACAAATCGT+TGG - Chr6:52395340-52395359 MsG0680032899.01.T01:three_prime_UTR 30.0%
ATGCTGAAATAGTTTCTGTT+GGG - Chr6:52398574-52398593 MsG0680032899.01.T01:intron 30.0%
ATTTGATTCTTCTTCACATC+AGG + Chr6:52398636-52398655 None:intergenic 30.0%
CAAAATCCACTTTAATGTGT+TGG + Chr6:52395610-52395629 None:intergenic 30.0%
CATATCCTTAAAATGGACAA+AGG + Chr6:52396931-52396950 None:intergenic 30.0%
CATGTAGTAATAATGAGGAT+AGG + Chr6:52398673-52398692 None:intergenic 30.0%
CCACTTTGATGAGAAATATT+AGG + Chr6:52395691-52395710 None:intergenic 30.0%
CCTAATATTTCTCATCAAAG+TGG - Chr6:52395688-52395707 MsG0680032899.01.T01:CDS 30.0%
GAATCACAAGTTTCTTGTAT+GGG + Chr6:52397289-52397308 None:intergenic 30.0%
GACAAGAAATTAACCTTAAC+TGG - Chr6:52398504-52398523 MsG0680032899.01.T01:intron 30.0%
GTAACATATGCTCATAAGAA+TGG - Chr6:52395426-52395445 MsG0680032899.01.T01:three_prime_UTR 30.0%
TACATGTAGTAAGCAAACTA+AGG - Chr6:52398541-52398560 MsG0680032899.01.T01:intron 30.0%
TAGTAAGCAAACTAAGGAAA+TGG - Chr6:52398547-52398566 MsG0680032899.01.T01:intron 30.0%
TCATGAATGAAAGATCTCAA+TGG - Chr6:52396273-52396292 MsG0680032899.01.T01:intron 30.0%
TGTTAACCAAAAACCAATGA+TGG + Chr6:52395667-52395686 None:intergenic 30.0%
TGTTGCATGTAGTAATAATG+AGG + Chr6:52398678-52398697 None:intergenic 30.0%
TTACGCAAATGAAATCATTC+AGG + Chr6:52396875-52396894 None:intergenic 30.0%
TTATTATCTCCGCCTAATAT+TGG + Chr6:52395885-52395904 None:intergenic 30.0%
TTTGAAAATGTTAGCAAGTG+AGG - Chr6:52397209-52397228 MsG0680032899.01.T01:intron 30.0%
! AATTGTTCACAATTTTCTCG+AGG + Chr6:52397115-52397134 None:intergenic 30.0%
! CGATTGATCTACCATATTTT+AGG + Chr6:52396093-52396112 None:intergenic 30.0%
! GTTACAGAAACTAGTATTGA+AGG - Chr6:52397782-52397801 MsG0680032899.01.T01:intron 30.0%
! TTCTTCTTTTCATCTTTTGC+AGG + Chr6:52398600-52398619 None:intergenic 30.0%
!! CAACACATTAAAGTGGATTT+TGG - Chr6:52395608-52395627 MsG0680032899.01.T01:CDS 30.0%
!! TTCGTTTTTCACTGTTTATG+AGG - Chr6:52398027-52398046 MsG0680032899.01.T01:intron 30.0%
!! TTCTTAGTGTGTTTGAGTTT+TGG - Chr6:52398354-52398373 MsG0680032899.01.T01:intron 30.0%
!! TTTCTCATCAAAGTGGATTT+TGG - Chr6:52395695-52395714 MsG0680032899.01.T01:CDS 30.0%
AAGATCTCAATGGAATGAGA+TGG - Chr6:52396283-52396302 MsG0680032899.01.T01:intron 35.0%
ACAATGAGCTTGTAACATGT+TGG - Chr6:52398858-52398877 MsG0680032899.01.T01:CDS 35.0%
ACTCAAGTGGTAAAAGATTG+CGG - Chr6:52398275-52398294 MsG0680032899.01.T01:intron 35.0%
AGTAAAAGAGGGAATTCACA+AGG + Chr6:52397715-52397734 None:intergenic 35.0%
AGTTGTATCACTTTCTTGGA+AGG - Chr6:52397412-52397431 MsG0680032899.01.T01:intron 35.0%
ATCAAGCCAACACATTAAAG+TGG - Chr6:52395601-52395620 MsG0680032899.01.T01:CDS 35.0%
CAACTTATTTACAGTCCAGT+AGG - Chr6:52395831-52395850 MsG0680032899.01.T01:intron 35.0%
CATGCTGAAATAGTTTCTGT+TGG - Chr6:52398573-52398592 MsG0680032899.01.T01:intron 35.0%
CTACTGGACTGTAAATAAGT+TGG + Chr6:52395833-52395852 None:intergenic 35.0%
GAATAAGAAAAGGAGCTTAG+AGG - Chr6:52396134-52396153 MsG0680032899.01.T01:intron 35.0%
GCGCGAAAATAGAATTAGTA+TGG - Chr6:52396540-52396559 MsG0680032899.01.T01:intron 35.0%
GTAGGCTGATTATCAATTGT+TGG - Chr6:52395849-52395868 MsG0680032899.01.T01:intron 35.0%
TAAAAGAAGAGCTAGGGTAT+TGG + Chr6:52397622-52397641 None:intergenic 35.0%
TATTACCGCGTTTAGTAGAA+TGG - Chr6:52397473-52397492 MsG0680032899.01.T01:intron 35.0%
TCTCAACCAAGCCTAAAATA+TGG - Chr6:52396079-52396098 MsG0680032899.01.T01:intron 35.0%
TGCAAACGAGTTATATCTTG+AGG + Chr6:52395977-52395996 None:intergenic 35.0%
TGTTCAATTGTGCAAACAGA+GGG - Chr6:52398908-52398927 MsG0680032899.01.T01:CDS 35.0%
TTCCTGCATCATAATAGATC+CGG + Chr6:52395385-52395404 None:intergenic 35.0%
TTGTTCCATTCTTGAAACAC+AGG + Chr6:52398721-52398740 None:intergenic 35.0%
TTTCACATCTTCAAAGTGTG+CGG + Chr6:52396498-52396517 None:intergenic 35.0%
! ACAAGTTGACATTACCAGAA+AGG + Chr6:52397860-52397879 None:intergenic 35.0%
! ACAATTGATAATCAGCCTAC+TGG + Chr6:52395849-52395868 None:intergenic 35.0%
! ACAGAAACTAGTATTGAAGG+TGG - Chr6:52397785-52397804 MsG0680032899.01.T01:intron 35.0%
! AGTCTTCATAGCTTTTTGCT+TGG + Chr6:52397829-52397848 None:intergenic 35.0%
! AGTTTGCTTACTACATGTAC+TGG + Chr6:52398540-52398559 None:intergenic 35.0%
! GACATCCTTTGTCCATTTTA+AGG - Chr6:52396923-52396942 MsG0680032899.01.T01:intron 35.0%
! GTTTTTCACTGTTTATGAGG+TGG - Chr6:52398030-52398049 MsG0680032899.01.T01:intron 35.0%
! TAAGCTCCTTTTCTTATTCG+AGG + Chr6:52396133-52396152 None:intergenic 35.0%
!! AAAACCAATGATGGATTAGG+AGG + Chr6:52395658-52395677 None:intergenic 35.0%
!! CCAAAAACCAATGATGGATT+AGG + Chr6:52395661-52395680 None:intergenic 35.0%
!! CGTCAATGTTGACTTCATTT+CGG + Chr6:52396311-52396330 None:intergenic 35.0%
!! GATCTACCATATTTTAGGCT+TGG + Chr6:52396088-52396107 None:intergenic 35.0%
!!! CCTAATCCATCATTGGTTTT+TGG - Chr6:52395658-52395677 MsG0680032899.01.T01:CDS 35.0%
AAAGCTATGAAGACTGTCTC+AGG - Chr6:52397834-52397853 MsG0680032899.01.T01:intron 40.0%
AATATATCAAGGCGCGTGTT+TGG - Chr6:52398306-52398325 MsG0680032899.01.T01:intron 40.0%
AGAATGGAACAAGATCCTGT+TGG - Chr6:52398729-52398748 MsG0680032899.01.T01:intron 40.0%
AGTTGTGAAGTGAAGATGAG+AGG + Chr6:52397560-52397579 None:intergenic 40.0%
ATATATCAAGGCGCGTGTTT+GGG - Chr6:52398307-52398326 MsG0680032899.01.T01:intron 40.0%
CAGTTCCAGATGAACTAATC+AGG - Chr6:52396218-52396237 MsG0680032899.01.T01:intron 40.0%
GTGAGGAATGCTCTTTGTTA+AGG - Chr6:52397226-52397245 MsG0680032899.01.T01:intron 40.0%
GTGTTCAATTGTGCAAACAG+AGG - Chr6:52398907-52398926 MsG0680032899.01.T01:CDS 40.0%
TAACTAGTCAACTCGAGTCA+AGG + Chr6:52397512-52397531 None:intergenic 40.0%
TCCTGCATCATAATAGATCC+GGG + Chr6:52395384-52395403 None:intergenic 40.0%
TCTTCCTCCTAATCCATCAT+TGG - Chr6:52395651-52395670 MsG0680032899.01.T01:CDS 40.0%
TTACAAATCGTTGGCAAGAC+CGG - Chr6:52395349-52395368 MsG0680032899.01.T01:three_prime_UTR 40.0%
TTTCTTGGAAGGAACAGTGT+GGG - Chr6:52397423-52397442 MsG0680032899.01.T01:intron 40.0%
! AGGTACCTGATTAGTTCATC+TGG + Chr6:52396226-52396245 None:intergenic 40.0%
! CTGATTAGTTCATCTGGAAC+TGG + Chr6:52396220-52396239 None:intergenic 40.0%
CAGCATATGACACATCCAAC+AGG + Chr6:52398747-52398766 None:intergenic 45.0%
CGGCATAAGTGTAACTCAAG+TGG - Chr6:52398262-52398281 MsG0680032899.01.T01:intron 45.0%
CTTTCTTGGAAGGAACAGTG+TGG - Chr6:52397422-52397441 MsG0680032899.01.T01:intron 45.0%
GTGGTCCATTCTACTAAACG+CGG + Chr6:52397481-52397500 None:intergenic 45.0%
TCCCGGATCTATTATGATGC+AGG - Chr6:52395380-52395399 MsG0680032899.01.T01:three_prime_UTR 45.0%
TCGAGTCAAGGAAAGTAGTG+TGG + Chr6:52397500-52397519 None:intergenic 45.0%
TGGATCTGTGTCTCCAGTTA+AGG + Chr6:52398520-52398539 None:intergenic 45.0%
TTCTTGGAAGGAACAGTGTG+GGG - Chr6:52397424-52397443 MsG0680032899.01.T01:intron 45.0%
!!! TATTTATTTTAATTGAAAAA+TGG + Chr6:52398123-52398142 None:intergenic 5.0%
AAATCGTTGGCAAGACCGGT+TGG - Chr6:52395353-52395372 MsG0680032899.01.T01:three_prime_UTR 50.0%
AAGACTGTCTCAGGCCTTTC+TGG - Chr6:52397843-52397862 MsG0680032899.01.T01:intron 50.0%
AATCGTTGGCAAGACCGGTT+GGG - Chr6:52395354-52395373 MsG0680032899.01.T01:three_prime_UTR 50.0%
TAGATCCGGGATAACCCAAC+CGG + Chr6:52395371-52395390 None:intergenic 50.0%
CAAGACCGGTTGGGTTATCC+CGG - Chr6:52395363-52395382 MsG0680032899.01.T01:three_prime_UTR 55.0%
Chromosome Type Strat End Strand Name
Chr6 gene 52395303 52398944 52395303 ID=MsG0680032899.01;Name=MsG0680032899.01
Chr6 mRNA 52395303 52398944 52395303 ID=MsG0680032899.01.T01;Parent=MsG0680032899.01;Name=MsG0680032899.01.T01;_AED=0.58;_eAED=0.59;_QI=0|0.5|0.33|0.66|0.5|0.33|3|177|171
Chr6 exon 52398822 52398944 52398822 ID=MsG0680032899.01.T01:exon:13407;Parent=MsG0680032899.01.T01
Chr6 exon 52396383 52396482 52396383 ID=MsG0680032899.01.T01:exon:13408;Parent=MsG0680032899.01.T01
Chr6 exon 52395303 52395772 52395303 ID=MsG0680032899.01.T01:exon:13409;Parent=MsG0680032899.01.T01
Chr6 CDS 52398822 52398944 52398822 ID=MsG0680032899.01.T01:cds;Parent=MsG0680032899.01.T01
Chr6 CDS 52396383 52396482 52396383 ID=MsG0680032899.01.T01:cds;Parent=MsG0680032899.01.T01
Chr6 CDS 52395480 52395772 52395480 ID=MsG0680032899.01.T01:cds;Parent=MsG0680032899.01.T01
Chr6 three_prime_UTR 52395303 52395479 52395303 ID=MsG0680032899.01.T01:three_prime_utr;Parent=MsG0680032899.01.T01
Gene Sequence

>MsG0680032899.01.T01

TATATTATTAGTGCAATCGAATCACATATTATTGTAAATCAGAAGTTTACAAATCGTTGGCAAGACCGGTTGGGTTATCCCGGATCTATTATGATGCAGGAACTAATTGATATTGAACAATTGATCTTTTGTAATGTTTTGTTACAGAAACTAGTATTGAAGGTGGATTTACATGATGACAGAACCAAGCAAAAAGCTATGAAGACTGTCTCAGGCCTTTCTGGGGTAGAGTCAGTTTCTGTAGACATGAAAGACAAGAAATTAACCTTAACTGGAGACACAGATCCAGTACATGTAGTAAGCAAACTAAGGAAATGGTGTCATGCTGAAATAGTTTCTGTTGGGCCTGCAAAAGATGAAAAGAAGAAAGAAGAATCTAAGCCTGATGTGAAGAAGAATCAAATTCAATTACTTGAAGCCTATCCTCATTATTACTACATGCAACAACCACAATATATTCAATATTCCTGTGTTTCAAGAATGGAACAAGATCCTGTTGGATGTGTCATATGCTGA
Protein sequence

>MsG0680032899.01.T01

YIISAIESHIIVNQKFTNRWQDRLGYPGSIMMQELIDIEQLIFCNVLLQKLVLKVDLHDDRTKQKAMKTVSGLSGVESVSVDMKDKKLTLTGDTDPVHVVSKLRKWCHAEIVSVGPAKDEKKKEESKPDVKKNQIQLLEAYPHYYYMQQPQYIQYSCVSRMEQDPVGCVIC*