AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880045185.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045185.01.T01 MTR_8g067280 95.625 160 5 1 1 160 491 648 2.13e-101 304
MsG0880045185.01.T01 MTR_5g030430 66.038 159 50 2 1 158 522 677 3.48e-60 197
MsG0880045185.01.T01 MTR_8g024790 39.073 151 72 2 1 147 320 454 6.61e-26 102
MsG0880045185.01.T01 MTR_8g024770 40.541 148 78 4 1 147 308 446 1.21e-23 95.9
MsG0880045185.01.T01 MTR_8g027495 30.921 152 83 4 1 151 487 617 9.23e-16 73.9
MsG0880045185.01.T01 MTR_7g117670 25.658 152 91 3 1 151 431 561 1.16e-12 65.1
MsG0880045185.01.T01 MTR_0194s0050 31.429 175 88 9 2 152 217 383 1.89e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045185.01.T01 AT4G17880 56.790 162 47 2 1 157 445 588 9.35e-51 171
MsG0880045185.01.T01 AT1G32640 59.355 155 51 2 1 155 481 623 3.98e-49 167
MsG0880045185.01.T01 AT5G46760 55.128 156 61 2 1 156 444 590 1.09e-48 165
MsG0880045185.01.T01 AT5G46830 49.359 156 60 5 1 154 372 510 4.61e-33 122
MsG0880045185.01.T01 AT4G00870 36.719 128 59 3 1 122 278 389 6.82e-15 71.2
MsG0880045185.01.T01 AT1G01260 30.464 151 68 2 1 151 462 575 6.94e-14 68.6
MsG0880045185.01.T01 AT1G01260 30.464 151 68 2 1 151 462 575 6.94e-14 68.6
MsG0880045185.01.T01 AT1G01260 30.464 151 68 2 1 151 462 575 6.94e-14 68.6

Find 27 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTTGTGCATCACCAATTT+TGG 0.145524 8:-55507206 None:intergenic
TCTCTTCTTTCTTCCAAAAT+TGG 0.173669 8:+55507193 MsG0880045185.01.T01:CDS
GGATATTGAAGTGAAGATTA+TGG 0.243770 8:+55506985 MsG0880045185.01.T01:CDS
CTCTTCTAAGTTAATTGACT+TGG 0.357805 8:+55506964 MsG0880045185.01.T01:CDS
AGGTGAATTGGAGAAACAAT+TGG 0.405166 8:+55506835 MsG0880045185.01.T01:CDS
TGGGTTGGGATGCTATGATT+AGG 0.406285 8:+55507005 MsG0880045185.01.T01:CDS
GATATTGAAGTGAAGATTAT+GGG 0.406522 8:+55506986 MsG0880045185.01.T01:CDS
GGAATCGTCAAAAGGTGAAT+TGG 0.427183 8:+55506823 MsG0880045185.01.T01:CDS
TCATCCTGCGGCGAAGTTGA+TGG 0.465184 8:+55507048 MsG0880045185.01.T01:CDS
GGGTGCAACAAAGAAGGAAC+TGG 0.478542 8:+55506856 MsG0880045185.01.T01:CDS
TGAAGTGAAGATTATGGGTT+GGG 0.485063 8:+55506991 MsG0880045185.01.T01:CDS
GCCGCCATCAACTTCGCCGC+AGG 0.505374 8:-55507052 None:intergenic
GAAATCGAAGCTACAAGGGC+TGG 0.510964 8:+55506802 MsG0880045185.01.T01:CDS
TCAACAAGCTTCTGTTAACA+TGG 0.511118 8:+55507138 MsG0880045185.01.T01:CDS
TTGAAGTGAAGATTATGGGT+TGG 0.525361 8:+55506990 MsG0880045185.01.T01:CDS
GGTGAATTGGAGAAACAATT+GGG 0.530956 8:+55506836 MsG0880045185.01.T01:CDS
TCCAAAATTGGTGATGCACA+AGG 0.537190 8:+55507205 MsG0880045185.01.T01:CDS
CAAGGGCTGGAATCGTCAAA+AGG 0.538390 8:+55506815 MsG0880045185.01.T01:CDS
AATTCCATTAGATAAAGAAA+AGG 0.545738 8:+55506919 MsG0880045185.01.T01:CDS
GAATTGAAATCGAAGCTACA+AGG 0.554225 8:+55506797 MsG0880045185.01.T01:CDS
ACAATTGGGTGCAACAAAGA+AGG 0.555028 8:+55506850 MsG0880045185.01.T01:CDS
ATGGACAAAGCATCGCTGCT+AGG 0.569855 8:+55506752 MsG0880045185.01.T01:CDS
GGCGTTGAAAGACTTAGACT+TGG 0.610935 8:+55507072 MsG0880045185.01.T01:CDS
CAACAAGCTTCTGTTAACAT+GGG 0.613453 8:+55507139 MsG0880045185.01.T01:CDS
TCCTGCGGCGAAGTTGATGG+CGG 0.639474 8:+55507051 MsG0880045185.01.T01:CDS
AATTGAAATCGAAGCTACAA+GGG 0.684780 8:+55506798 MsG0880045185.01.T01:CDS
CAGCAAGAAGAATCATCCTG+CGG 0.713584 8:+55507036 MsG0880045185.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTCCATTAGATAAAGAAA+AGG + Chr8:55506919-55506938 MsG0880045185.01.T01:CDS 20.0%
!!! TTTTCTTTATCTAATGGAAT+TGG - Chr8:55506920-55506939 None:intergenic 20.0%
! GATATTGAAGTGAAGATTAT+GGG + Chr8:55506986-55507005 MsG0880045185.01.T01:CDS 25.0%
!! TTCTCCTTTTCTTTATCTAA+TGG - Chr8:55506926-55506945 None:intergenic 25.0%
AATTGAAATCGAAGCTACAA+GGG + Chr8:55506798-55506817 MsG0880045185.01.T01:CDS 30.0%
CTCTTCTAAGTTAATTGACT+TGG + Chr8:55506964-55506983 MsG0880045185.01.T01:CDS 30.0%
GGATATTGAAGTGAAGATTA+TGG + Chr8:55506985-55507004 MsG0880045185.01.T01:CDS 30.0%
TCTCTTCTTTCTTCCAAAAT+TGG + Chr8:55507193-55507212 MsG0880045185.01.T01:CDS 30.0%
AGGTGAATTGGAGAAACAAT+TGG + Chr8:55506835-55506854 MsG0880045185.01.T01:CDS 35.0%
CAACAAGCTTCTGTTAACAT+GGG + Chr8:55507139-55507158 MsG0880045185.01.T01:CDS 35.0%
GAATTGAAATCGAAGCTACA+AGG + Chr8:55506797-55506816 MsG0880045185.01.T01:CDS 35.0%
GGTGAATTGGAGAAACAATT+GGG + Chr8:55506836-55506855 MsG0880045185.01.T01:CDS 35.0%
TCAACAAGCTTCTGTTAACA+TGG + Chr8:55507138-55507157 MsG0880045185.01.T01:CDS 35.0%
TGAAGTGAAGATTATGGGTT+GGG + Chr8:55506991-55507010 MsG0880045185.01.T01:CDS 35.0%
TTGAAGTGAAGATTATGGGT+TGG + Chr8:55506990-55507009 MsG0880045185.01.T01:CDS 35.0%
ACAATTGGGTGCAACAAAGA+AGG + Chr8:55506850-55506869 MsG0880045185.01.T01:CDS 40.0%
GGAATCGTCAAAAGGTGAAT+TGG + Chr8:55506823-55506842 MsG0880045185.01.T01:CDS 40.0%
! TCCTTGTGCATCACCAATTT+TGG - Chr8:55507209-55507228 None:intergenic 40.0%
!! TCCAAAATTGGTGATGCACA+AGG + Chr8:55507205-55507224 MsG0880045185.01.T01:CDS 40.0%
CAGCAAGAAGAATCATCCTG+CGG + Chr8:55507036-55507055 MsG0880045185.01.T01:CDS 45.0%
! GGCGTTGAAAGACTTAGACT+TGG + Chr8:55507072-55507091 MsG0880045185.01.T01:CDS 45.0%
! TGGGTTGGGATGCTATGATT+AGG + Chr8:55507005-55507024 MsG0880045185.01.T01:CDS 45.0%
CAAGGGCTGGAATCGTCAAA+AGG + Chr8:55506815-55506834 MsG0880045185.01.T01:CDS 50.0%
GAAATCGAAGCTACAAGGGC+TGG + Chr8:55506802-55506821 MsG0880045185.01.T01:CDS 50.0%
GGGTGCAACAAAGAAGGAAC+TGG + Chr8:55506856-55506875 MsG0880045185.01.T01:CDS 50.0%
!! ATGGACAAAGCATCGCTGCT+AGG + Chr8:55506752-55506771 MsG0880045185.01.T01:CDS 50.0%
TCATCCTGCGGCGAAGTTGA+TGG + Chr8:55507048-55507067 MsG0880045185.01.T01:CDS 55.0%
!! TCCTGCGGCGAAGTTGATGG+CGG + Chr8:55507051-55507070 MsG0880045185.01.T01:CDS 60.0%
GCCGCCATCAACTTCGCCGC+AGG - Chr8:55507055-55507074 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr8 gene 55506752 55507234 55506752 ID=MsG0880045185.01;Name=MsG0880045185.01
Chr8 mRNA 55506752 55507234 55506752 ID=MsG0880045185.01.T01;Parent=MsG0880045185.01;Name=MsG0880045185.01.T01;_AED=0.42;_eAED=0.42;_QI=0|-1|0|1|-1|1|1|0|160
Chr8 exon 55506752 55507234 55506752 ID=MsG0880045185.01.T01:exon:23924;Parent=MsG0880045185.01.T01
Chr8 CDS 55506752 55507234 55506752 ID=MsG0880045185.01.T01:cds;Parent=MsG0880045185.01.T01
Gene Sequence

>MsG0880045185.01.T01

ATGGACAAAGCATCGCTGCTAGGCGATGCTATTTCTTATATAAACGAATTGAAATCGAAGCTACAAGGGCTGGAATCGTCAAAAGGTGAATTGGAGAAACAATTGGGTGCAACAAAGAAGGAACTGGAACTTGTTACTCGTAAAAATCAAAATCAAAATCAAAATCCAATTCCATTAGATAAAGAAAAGGAGAAAACAACAAGCTCAACAAGCTCTTCTAAGTTAATTGACTTGGATATTGAAGTGAAGATTATGGGTTGGGATGCTATGATTAGGATTCAATGCAGCAAGAAGAATCATCCTGCGGCGAAGTTGATGGCGGCGTTGAAAGACTTAGACTTGGATGTTAATCATGCTAGTGTGTCTGTTGTGAATGATTTGATGATTCAACAAGCTTCTGTTAACATGGGAAGTCGATTTTACACACAAGAACAACTTTTGTCTCTTCTTTCTTCCAAAATTGGTGATGCACAAGGAGATTGA

Protein sequence

>MsG0880045185.01.T01

MDKASLLGDAISYINELKSKLQGLESSKGELEKQLGATKKELELVTRKNQNQNQNPIPLDKEKEKTTSSTSSSKLIDLDIEVKIMGWDAMIRIQCSKKNHPAAKLMAALKDLDLDVNHASVSVVNDLMIQQASVNMGSRFYTQEQLLSLLSSKIGDAQGD*