Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010378.01.T01 | XP_003596635.1 | 92.929 | 198 | 11 | 1 | 1 | 198 | 23 | 217 | 2.21E-127 | 367 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010378.01.T01 | O48915 | 41.237 | 194 | 106 | 5 | 2 | 191 | 29 | 218 | 6.10E-37 | 130 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010378.01.T01 | G7IJL6 | 92.929 | 198 | 11 | 1 | 1 | 198 | 23 | 217 | 1.05e-127 | 367 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048683.01 | MsG0280010378.01 | 0.820431 | 6.886001e-53 | 5.447652e-50 |
| MsG0080049147.01 | MsG0280010378.01 | 0.809412 | 1.916452e-50 | 1.124482e-47 |
| MsG0180000157.01 | MsG0280010378.01 | 0.820155 | 7.963131e-53 | 6.251926e-50 |
| MsG0180000240.01 | MsG0280010378.01 | -0.817617 | 3.004574e-52 | 2.198812e-49 |
| MsG0180000470.01 | MsG0280010378.01 | 0.810266 | 1.255711e-50 | 7.534897e-48 |
| MsG0180000650.01 | MsG0280010378.01 | 0.814505 | 1.488617e-51 | 1.000734e-48 |
| MsG0180001122.01 | MsG0280010378.01 | 0.803733 | 3.027835e-49 | 1.533157e-46 |
| MsG0180001712.01 | MsG0280010378.01 | 0.805460 | 1.321001e-49 | 6.992841e-47 |
| MsG0180002977.01 | MsG0280010378.01 | -0.804208 | 2.412485e-49 | 1.236507e-46 |
| MsG0180004390.01 | MsG0280010378.01 | 0.822499 | 2.292636e-53 | 1.922336e-50 |
| MsG0180004765.01 | MsG0280010378.01 | 0.807425 | 5.087302e-50 | 2.832602e-47 |
| MsG0180005279.01 | MsG0280010378.01 | 0.873363 | 1.558145e-67 | 6.743590e-64 |
| MsG0180005303.01 | MsG0280010378.01 | -0.817931 | 2.552742e-52 | 1.884374e-49 |
| MsG0180005505.01 | MsG0280010378.01 | 0.820845 | 5.531010e-53 | 4.426262e-50 |
| MsG0180005507.01 | MsG0280010378.01 | 0.811346 | 7.329257e-51 | 4.525616e-48 |
| MsG0180005515.01 | MsG0280010378.01 | -0.828105 | 1.081554e-54 | 1.064690e-51 |
| MsG0180006064.01 | MsG0280010378.01 | -0.813760 | 2.174792e-51 | 1.432634e-48 |
| MsG0280007547.01 | MsG0280010378.01 | 0.843928 | 1.036440e-58 | 1.646894e-55 |
| MsG0280007714.01 | MsG0280010378.01 | 0.825435 | 4.695993e-54 | 4.279639e-51 |
| MsG0280007826.01 | MsG0280010378.01 | 0.811608 | 6.428863e-51 | 3.997273e-48 |
| MsG0280008220.01 | MsG0280010378.01 | 0.814476 | 1.511207e-51 | 1.015098e-48 |
| MsG0280008295.01 | MsG0280010378.01 | 0.817656 | 2.944821e-52 | 2.157437e-49 |
| MsG0280010378.01 | MsG0280010443.01 | 0.804926 | 1.708901e-49 | 8.923448e-47 |
| MsG0280010378.01 | MsG0280011052.01 | 0.803905 | 2.788820e-49 | 1.418446e-46 |
| MsG0280010378.01 | MsG0280011163.01 | 0.849328 | 3.469698e-60 | 6.555107e-57 |
| MsG0280010378.01 | MsG0280011376.01 | 0.812093 | 5.041303e-51 | 3.175627e-48 |
| MsG0280010378.01 | MsG0380011754.01 | 0.812626 | 3.856652e-51 | 2.464303e-48 |
| MsG0280010378.01 | MsG0380012005.01 | 0.818668 | 1.738063e-52 | 1.309276e-49 |
| MsG0280010378.01 | MsG0380012007.01 | 0.821387 | 4.148980e-53 | 3.370627e-50 |
| MsG0280010378.01 | MsG0380013853.01 | 0.807669 | 4.515032e-50 | 2.529876e-47 |
| MsG0280010378.01 | MsG0380014866.01 | 0.820995 | 5.107256e-53 | 4.104645e-50 |
| MsG0280010378.01 | MsG0380014902.01 | 0.809517 | 1.819429e-50 | 1.070495e-47 |
| MsG0280010378.01 | MsG0380015215.01 | -0.802215 | 6.235262e-49 | 3.037005e-46 |
| MsG0280010378.01 | MsG0380015289.01 | 0.836302 | 1.013099e-56 | 1.271738e-53 |
| MsG0280010378.01 | MsG0380015878.01 | 0.820904 | 5.361621e-53 | 4.297740e-50 |
| MsG0280010378.01 | MsG0380016004.01 | 0.831169 | 1.943947e-55 | 2.092714e-52 |
| MsG0280010378.01 | MsG0380016182.01 | 0.806914 | 6.526578e-50 | 3.586026e-47 |
| MsG0280010378.01 | MsG0380016191.01 | -0.842556 | 2.405580e-58 | 3.661024e-55 |
| MsG0280010378.01 | MsG0380016828.01 | -0.821141 | 4.726463e-53 | 3.813768e-50 |
| MsG0280010378.01 | MsG0380016910.01 | 0.806312 | 8.743514e-50 | 4.730522e-47 |
| MsG0280010378.01 | MsG0380017085.01 | 0.808246 | 3.403984e-50 | 1.936604e-47 |
| MsG0280010378.01 | MsG0380017178.01 | -0.828532 | 8.532357e-55 | 8.504130e-52 |
| MsG0280010378.01 | MsG0380017220.01 | 0.807655 | 4.546305e-50 | 2.546473e-47 |
| MsG0280010378.01 | MsG0380017263.01 | -0.823915 | 1.071171e-53 | 9.348535e-51 |
| MsG0280010378.01 | MsG0380017286.01 | 0.808150 | 3.568069e-50 | 2.024803e-47 |
| MsG0280010378.01 | MsG0380017322.01 | 0.821688 | 3.534768e-53 | 2.896032e-50 |
| MsG0280010378.01 | MsG0380017746.01 | 0.816291 | 5.964875e-52 | 4.209966e-49 |
| MsG0280010378.01 | MsG0380017941.01 | 0.854725 | 1.016292e-61 | 2.291302e-58 |
| MsG0280010378.01 | MsG0480018321.01 | 0.827862 | 1.237512e-54 | 1.209586e-51 |
| MsG0280010378.01 | MsG0480018603.01 | 0.821315 | 4.309702e-53 | 3.494194e-50 |
| MsG0280010378.01 | MsG0480019871.01 | 0.816700 | 4.829826e-52 | 3.446922e-49 |
| MsG0280010378.01 | MsG0480020024.01 | 0.801056 | 1.077747e-48 | 5.097959e-46 |
| MsG0280010378.01 | MsG0480020449.01 | 0.802970 | 4.357813e-49 | 2.163711e-46 |
| MsG0280010378.01 | MsG0480020839.01 | 0.808073 | 3.704148e-50 | 2.097672e-47 |
| MsG0280010378.01 | MsG0480020887.01 | 0.846749 | 1.786087e-59 | 3.105458e-56 |
| MsG0280010378.01 | MsG0480020964.01 | 0.807902 | 4.028954e-50 | 2.271196e-47 |
| MsG0280010378.01 | MsG0480021021.01 | 0.807704 | 4.438384e-50 | 2.489253e-47 |
| MsG0280010378.01 | MsG0480021869.01 | 0.801851 | 7.407672e-49 | 3.574909e-46 |
| MsG0280010378.01 | MsG0480021870.01 | 0.807287 | 5.441037e-50 | 3.018789e-47 |
| MsG0280010378.01 | MsG0480021927.01 | 0.846272 | 2.410779e-59 | 4.126967e-56 |
| MsG0280010378.01 | MsG0480022406.01 | 0.805730 | 1.159024e-49 | 6.178605e-47 |
| MsG0280010378.01 | MsG0480022451.01 | 0.800199 | 1.612248e-48 | 7.461418e-46 |
| MsG0280010378.01 | MsG0480022533.01 | 0.833030 | 6.739775e-56 | 7.667887e-53 |
| MsG0280010378.01 | MsG0480022575.01 | 0.842337 | 2.750328e-58 | 4.156992e-55 |
| MsG0280010378.01 | MsG0480023099.01 | 0.882088 | 1.396671e-70 | 8.393255e-67 |
| MsG0280010378.01 | MsG0480023248.01 | 0.848866 | 4.663426e-60 | 8.678309e-57 |
| MsG0280010378.01 | MsG0480023251.01 | 0.816051 | 6.750708e-52 | 4.733353e-49 |
| MsG0280010378.01 | MsG0480023503.01 | 0.800030 | 1.744815e-48 | 8.040563e-46 |
| MsG0280010378.01 | MsG0480023670.01 | 0.801917 | 7.181738e-49 | 3.471570e-46 |
| MsG0280010378.01 | MsG0480023731.01 | 0.825943 | 3.558243e-54 | 3.290460e-51 |
| MsG0280010378.01 | MsG0580024363.01 | -0.853312 | 2.596587e-61 | 5.586635e-58 |
| MsG0280010378.01 | MsG0580024494.01 | 0.802272 | 6.070378e-49 | 2.961042e-46 |
| MsG0280010378.01 | MsG0580024596.01 | 0.819774 | 9.735760e-53 | 7.562964e-50 |
| MsG0280010378.01 | MsG0580024853.01 | 0.819350 | 1.216051e-52 | 9.334161e-50 |
| MsG0280010378.01 | MsG0580025278.01 | 0.817038 | 4.056536e-52 | 2.922071e-49 |
| MsG0280010378.01 | MsG0580025453.01 | 0.811973 | 5.354233e-51 | 3.361775e-48 |
| MsG0280010378.01 | MsG0580025739.01 | 0.809006 | 2.342193e-50 | 1.359329e-47 |
| MsG0280010378.01 | MsG0580027103.01 | 0.824919 | 6.216093e-54 | 5.583307e-51 |
| MsG0280010378.01 | MsG0580027401.01 | 0.856320 | 3.484148e-62 | 8.283133e-59 |
| MsG0280010378.01 | MsG0580027833.01 | 0.820038 | 8.469420e-53 | 6.628040e-50 |
| MsG0280010378.01 | MsG0580028527.01 | 0.814477 | 1.510385e-51 | 1.014568e-48 |
| MsG0280010378.01 | MsG0580029999.01 | -0.804714 | 1.891976e-49 | 9.825127e-47 |
| MsG0280010378.01 | MsG0680030629.01 | 0.862101 | 6.430161e-64 | 1.860962e-60 |
| MsG0280010378.01 | MsG0680030636.01 | 0.802305 | 5.976511e-49 | 2.917843e-46 |
| MsG0280010378.01 | MsG0680030992.01 | 0.818664 | 1.741946e-52 | 1.311995e-49 |
| MsG0280010378.01 | MsG0680031459.01 | 0.857281 | 1.816203e-62 | 4.458802e-59 |
| MsG0280010378.01 | MsG0680032058.01 | 0.857063 | 2.106487e-62 | 5.133599e-59 |
| MsG0280010378.01 | MsG0680032204.01 | 0.800766 | 1.235518e-48 | 5.800789e-46 |
| MsG0280010378.01 | MsG0680032460.01 | -0.833207 | 6.088333e-56 | 6.963340e-53 |
| MsG0280010378.01 | MsG0680032896.01 | 0.862629 | 4.427298e-64 | 1.305113e-60 |
| MsG0280010378.01 | MsG0680032899.01 | 0.869782 | 2.391525e-66 | 9.084478e-63 |
| MsG0280010378.01 | MsG0680032996.01 | 0.841524 | 4.510793e-58 | 6.645887e-55 |
| MsG0280010378.01 | MsG0680033465.01 | 0.815387 | 9.491105e-52 | 6.534472e-49 |
| MsG0280010378.01 | MsG0680033926.01 | 0.800906 | 1.157034e-48 | 5.452046e-46 |
| MsG0280010378.01 | MsG0680034036.01 | 0.828353 | 9.426021e-55 | 9.345635e-52 |
| MsG0280010378.01 | MsG0680034655.01 | 0.810875 | 9.271530e-51 | 5.654717e-48 |
| MsG0280010378.01 | MsG0680035444.01 | 0.820337 | 7.233781e-53 | 5.707700e-50 |
| MsG0280010378.01 | MsG0680035445.01 | 0.856368 | 3.373881e-62 | 8.034444e-59 |
| MsG0280010378.01 | MsG0680035454.01 | 0.803796 | 2.937449e-49 | 1.489797e-46 |
| MsG0280010378.01 | MsG0680035844.01 | 0.819984 | 8.715625e-53 | 6.810296e-50 |
| MsG0280010378.01 | MsG0780036291.01 | 0.847508 | 1.106097e-59 | 1.970413e-56 |
| MsG0280010378.01 | MsG0780036630.01 | 0.825783 | 3.883386e-54 | 3.574800e-51 |
| MsG0280010378.01 | MsG0780037859.01 | 0.816710 | 4.805638e-52 | 3.430560e-49 |
| MsG0280010378.01 | MsG0780038825.01 | 0.805037 | 1.619832e-49 | 8.481766e-47 |
| MsG0280010378.01 | MsG0780038828.01 | 0.818323 | 2.081047e-52 | 1.552798e-49 |
| MsG0280010378.01 | MsG0780038829.01 | 0.803582 | 3.254451e-49 | 1.641620e-46 |
| MsG0280010378.01 | MsG0780038833.01 | 0.838211 | 3.289803e-57 | 4.375724e-54 |
| MsG0280010378.01 | MsG0780038836.01 | 0.801866 | 7.356666e-49 | 3.551641e-46 |
| MsG0280010378.01 | MsG0780039867.01 | -0.818010 | 2.449503e-52 | 1.812036e-49 |
| MsG0280010378.01 | MsG0780039875.01 | 0.817160 | 3.808191e-52 | 2.752353e-49 |
| MsG0280010378.01 | MsG0780040148.01 | -0.819678 | 1.023886e-52 | 7.931825e-50 |
| MsG0280010378.01 | MsG0780040389.01 | 0.825985 | 3.476357e-54 | 3.218571e-51 |
| MsG0280010378.01 | MsG0780040576.01 | -0.850386 | 1.755553e-60 | 3.433121e-57 |
| MsG0280010378.01 | MsG0780040591.01 | 0.816433 | 5.544299e-52 | 3.927889e-49 |
| MsG0280010378.01 | MsG0780040596.01 | 0.815356 | 9.640119e-52 | 6.631539e-49 |
| MsG0280010378.01 | MsG0780040679.01 | 0.803999 | 2.666610e-49 | 1.359542e-46 |
| MsG0280010378.01 | MsG0780040726.01 | 0.822863 | 1.886250e-53 | 1.598040e-50 |
| MsG0280010378.01 | MsG0780041441.01 | 0.826574 | 2.517609e-54 | 2.370110e-51 |
| MsG0280010378.01 | MsG0780041777.01 | 0.802046 | 6.754123e-49 | 3.275636e-46 |
| MsG0280010378.01 | MsG0880042053.01 | 0.801284 | 9.680210e-49 | 4.605123e-46 |
| MsG0280010378.01 | MsG0880042118.01 | 0.847273 | 1.283911e-59 | 2.269947e-56 |
| MsG0280010378.01 | MsG0880042161.01 | 0.833667 | 4.675611e-56 | 5.421398e-53 |
| MsG0280010378.01 | MsG0880042276.01 | 0.808570 | 2.902153e-50 | 1.665094e-47 |
| MsG0280010378.01 | MsG0880042434.01 | 0.813314 | 2.725963e-51 | 1.774152e-48 |
| MsG0280010378.01 | MsG0880042611.01 | 0.813248 | 2.817121e-51 | 1.830106e-48 |
| MsG0280010378.01 | MsG0880042656.01 | 0.832409 | 9.612979e-56 | 1.073579e-52 |
| MsG0280010378.01 | MsG0880043489.01 | 0.802738 | 4.865537e-49 | 2.401730e-46 |
| MsG0280010378.01 | MsG0880043584.01 | 0.848137 | 7.421383e-60 | 1.348578e-56 |
| MsG0280010378.01 | MsG0880044389.01 | -0.817149 | 3.830604e-52 | 2.767634e-49 |
| MsG0280010378.01 | MsG0880044573.01 | 0.812077 | 5.083448e-51 | 3.200749e-48 |
| MsG0280010378.01 | MsG0880045371.01 | 0.801750 | 7.769044e-49 | 3.739495e-46 |
| MsG0280010378.01 | MsG0880045605.01 | -0.820868 | 5.463395e-53 | 4.375125e-50 |
| MsG0280010378.01 | MsG0880045756.01 | 0.836275 | 1.029787e-56 | 1.291580e-53 |
| MsG0280010378.01 | MsG0880045765.01 | 0.838537 | 2.710732e-57 | 3.641705e-54 |
| MsG0280010378.01 | MsG0880045803.01 | 0.821528 | 3.848683e-53 | 3.138913e-50 |
| MsG0280010378.01 | MsG0880045926.01 | 0.852938 | 3.322364e-61 | 7.057879e-58 |
| MsG0280010378.01 | MsG0880046269.01 | 0.809586 | 1.758692e-50 | 1.036703e-47 |
| MsG0280010378.01 | MsG0880046440.01 | 0.801279 | 9.703159e-49 | 4.615482e-46 |
| MsG0280010378.01 | MsG0880046731.01 | 0.850840 | 1.308552e-60 | 2.597433e-57 |
| MsG0280010378.01 | MsG0880046809.01 | 0.828375 | 9.307786e-55 | 9.235008e-52 |
| MsG0280010378.01 | MsG0880046967.01 | 0.822043 | 2.925728e-53 | 2.421234e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0480022043.01 | MsG0280010378.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010378.01.T01 | MTR_2g082990 | 92.929 | 198 | 11 | 1 | 1 | 198 | 23 | 217 | 2.67e-131 | 367 |
| MsG0280010378.01.T01 | MTR_4g081200 | 48.387 | 186 | 90 | 4 | 3 | 185 | 28 | 210 | 2.62e-50 | 162 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010378.01.T01 | AT3G20600 | 41.237 | 194 | 106 | 5 | 2 | 191 | 29 | 218 | 6.22e-38 | 130 |
| MsG0280010378.01.T01 | AT3G20590 | 40.761 | 184 | 94 | 5 | 2 | 180 | 29 | 202 | 9.90e-30 | 110 |
| MsG0280010378.01.T01 | AT3G20610 | 34.158 | 202 | 119 | 7 | 1 | 195 | 27 | 221 | 7.45e-28 | 105 |
Find 55 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAATACCTTTATCTTTATT+AGG | 0.156001 | 2:+68789926 | None:intergenic |
| TGCGGTTAAGTATAAGATTT+TGG | 0.193625 | 2:-68789696 | MsG0280010378.01.T01:CDS |
| TGGAGATTCTGGGGAGAAAA+TGG | 0.196345 | 2:-68789624 | MsG0280010378.01.T01:CDS |
| CTTGTTAATGCTACTGTAAA+TGG | 0.211165 | 2:-68789845 | MsG0280010378.01.T01:CDS |
| TGGGTCTTGTTTATTGGTCT+TGG | 0.228865 | 2:-68789506 | MsG0280010378.01.T01:CDS |
| TGATCTTGCTGTTGTGTTTA+GGG | 0.236469 | 2:+68789985 | None:intergenic |
| AATGTGGAAATTGGAGATTC+TGG | 0.246829 | 2:-68789635 | MsG0280010378.01.T01:CDS |
| GAATACCTTTATCTTTATTA+GGG | 0.259706 | 2:+68789927 | None:intergenic |
| TTGATCTTGCTGTTGTGTTT+AGG | 0.268959 | 2:+68789984 | None:intergenic |
| ATCGTGCGCTTCTTGTGTTT+TGG | 0.270001 | 2:-68789526 | MsG0280010378.01.T01:CDS |
| TTGATGGTAAAGTTTATCTA+AGG | 0.284290 | 2:-68789733 | MsG0280010378.01.T01:CDS |
| GGAGGGGCAAATGTGGAAAT+TGG | 0.299189 | 2:-68789644 | MsG0280010378.01.T01:CDS |
| AAGGTGAAGTTAAGATGAAT+AGG | 0.325470 | 2:+68789896 | None:intergenic |
| GGGTCTTGTTTATTGGTCTT+GGG | 0.348639 | 2:-68789505 | MsG0280010378.01.T01:CDS |
| AATTTAACTGCGAGTGTTAA+GGG | 0.363654 | 2:-68789761 | MsG0280010378.01.T01:CDS |
| GTATGCTTAGCCATATGAAT+AGG | 0.380130 | 2:+68790065 | None:intergenic |
| GTTAACCCTAATAAAGATAA+AGG | 0.389560 | 2:-68789932 | MsG0280010378.01.T01:CDS |
| GAATTTAACTGCGAGTGTTA+AGG | 0.395995 | 2:-68789762 | MsG0280010378.01.T01:CDS |
| AGTGTTGAGAGTATTGTAAA+AGG | 0.426836 | 2:+68789877 | None:intergenic |
| ACTGTAAATGGATTCTATCA+AGG | 0.430123 | 2:-68789833 | MsG0280010378.01.T01:CDS |
| GGAGATTCTGGGGAGAAAAT+GGG | 0.447531 | 2:-68789623 | MsG0280010378.01.T01:CDS |
| AAAGAGATCGGGTATGGGGA+GGG | 0.451284 | 2:-68789661 | MsG0280010378.01.T01:CDS |
| AGTGTTAAGGGTGTTGTTGA+TGG | 0.454047 | 2:-68789749 | MsG0280010378.01.T01:CDS |
| GCTTAATGGCCCTATTCATA+TGG | 0.461217 | 2:-68790075 | None:intergenic |
| ATGTGGAAATTGGAGATTCT+GGG | 0.462099 | 2:-68789634 | MsG0280010378.01.T01:CDS |
| AGGTGAAGTTAAGATGAATA+GGG | 0.473108 | 2:+68789897 | None:intergenic |
| GGTATGGGGAGGGGCAAATG+TGG | 0.473413 | 2:-68789651 | MsG0280010378.01.T01:CDS |
| TATGCTTAGCCATATGAATA+GGG | 0.474917 | 2:+68790066 | None:intergenic |
| AAGAAAAGTGTGAGGCTTGG+AGG | 0.475890 | 2:-68789599 | MsG0280010378.01.T01:CDS |
| ATACGAAAAGAGATCGGGTA+TGG | 0.508627 | 2:-68789667 | MsG0280010378.01.T01:CDS |
| AAGAATGGAGTGGTGGAAGC+TGG | 0.512376 | 2:-68789791 | MsG0280010378.01.T01:CDS |
| TCCGGCGCATGGAAAGTGTA+TGG | 0.512912 | 2:-68789554 | MsG0280010378.01.T01:CDS |
| GAACCAAGGGTGATAGTGTC+CGG | 0.513905 | 2:-68789572 | MsG0280010378.01.T01:CDS |
| AAGAGATCGGGTATGGGGAG+GGG | 0.523912 | 2:-68789660 | MsG0280010378.01.T01:CDS |
| AGAAAAGTGTGAGGCTTGGA+GGG | 0.526565 | 2:-68789598 | MsG0280010378.01.T01:CDS |
| GGGTGATAGTGTCCGGCGCA+TGG | 0.534538 | 2:-68789565 | MsG0280010378.01.T01:CDS |
| TACGAAAAGAGATCGGGTAT+GGG | 0.536489 | 2:-68789666 | MsG0280010378.01.T01:CDS |
| TCCATACACTTTCCATGCGC+CGG | 0.542433 | 2:+68789553 | None:intergenic |
| GGAAAGAAAAGTGTGAGGCT+TGG | 0.543228 | 2:-68789602 | MsG0280010378.01.T01:CDS |
| TTGGTATACGAAAAGAGATC+GGG | 0.544628 | 2:-68789672 | MsG0280010378.01.T01:CDS |
| AGCTAAGAAGAATGGAGTGG+TGG | 0.547361 | 2:-68789798 | MsG0280010378.01.T01:CDS |
| AAAAGAGATCGGGTATGGGG+AGG | 0.556071 | 2:-68789662 | MsG0280010378.01.T01:CDS |
| GCGCCGGACACTATCACCCT+TGG | 0.558967 | 2:+68789569 | None:intergenic |
| CTGTAAATGGATTCTATCAA+GGG | 0.559809 | 2:-68789832 | MsG0280010378.01.T01:CDS |
| TTTGGTATACGAAAAGAGAT+CGG | 0.560749 | 2:-68789673 | MsG0280010378.01.T01:CDS |
| TGGTAAAGTTTATCTAAGGG+TGG | 0.560905 | 2:-68789729 | MsG0280010378.01.T01:CDS |
| TGATGGTAAAGTTTATCTAA+GGG | 0.567329 | 2:-68789732 | MsG0280010378.01.T01:CDS |
| TTTACAGTAGCATTAACAAG+TGG | 0.593952 | 2:+68789848 | None:intergenic |
| ACGAAAAGAGATCGGGTATG+GGG | 0.597221 | 2:-68789665 | MsG0280010378.01.T01:CDS |
| GGCTTGGAGGGAATGAACCA+AGG | 0.634018 | 2:-68789586 | MsG0280010378.01.T01:CDS |
| GCTTGGAGGGAATGAACCAA+GGG | 0.643282 | 2:-68789585 | MsG0280010378.01.T01:CDS |
| TGTGGAAATTGGAGATTCTG+GGG | 0.648415 | 2:-68789633 | MsG0280010378.01.T01:CDS |
| AAAAGCTAAGAAGAATGGAG+TGG | 0.661701 | 2:-68789801 | MsG0280010378.01.T01:CDS |
| AAATGGGAAAGAAAAGTGTG+AGG | 0.693699 | 2:-68789607 | MsG0280010378.01.T01:CDS |
| GGCGCATGGAAAGTGTATGG+AGG | 0.700562 | 2:-68789551 | MsG0280010378.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GAATACCTTTATCTTTATTA+GGG | + | Chr2:68789642-68789661 | None:intergenic | 20.0% |
| !! | TGAATACCTTTATCTTTATT+AGG | + | Chr2:68789643-68789662 | None:intergenic | 20.0% |
| !!! | TTAAGTATAAGATTTTGGTT+TGG | - | Chr2:68789875-68789894 | MsG0280010378.01.T01:CDS | 20.0% |
| ! | GTTAACCCTAATAAAGATAA+AGG | - | Chr2:68789634-68789653 | MsG0280010378.01.T01:CDS | 25.0% |
| ! | TGATGGTAAAGTTTATCTAA+GGG | - | Chr2:68789834-68789853 | MsG0280010378.01.T01:CDS | 25.0% |
| !! | ATTTTGTCTATGTAAAGCTT+TGG | + | Chr2:68789538-68789557 | None:intergenic | 25.0% |
| !! | TTGATGGTAAAGTTTATCTA+AGG | - | Chr2:68789833-68789852 | MsG0280010378.01.T01:CDS | 25.0% |
| AAGGTGAAGTTAAGATGAAT+AGG | + | Chr2:68789673-68789692 | None:intergenic | 30.0% | |
| AATTTAACTGCGAGTGTTAA+GGG | - | Chr2:68789805-68789824 | MsG0280010378.01.T01:CDS | 30.0% | |
| AGGTGAAGTTAAGATGAATA+GGG | + | Chr2:68789672-68789691 | None:intergenic | 30.0% | |
| CTTGTTAATGCTACTGTAAA+TGG | - | Chr2:68789721-68789740 | MsG0280010378.01.T01:CDS | 30.0% | |
| GAGAAAAAAGCTAAGAAGAA+TGG | - | Chr2:68789760-68789779 | MsG0280010378.01.T01:CDS | 30.0% | |
| TATGCTTAGCCATATGAATA+GGG | + | Chr2:68789503-68789522 | None:intergenic | 30.0% | |
| TTTACAGTAGCATTAACAAG+TGG | + | Chr2:68789721-68789740 | None:intergenic | 30.0% | |
| TTTGGTATACGAAAAGAGAT+CGG | - | Chr2:68789893-68789912 | MsG0280010378.01.T01:CDS | 30.0% | |
| ! | ACTGTAAATGGATTCTATCA+AGG | - | Chr2:68789733-68789752 | MsG0280010378.01.T01:CDS | 30.0% |
| ! | CTGTAAATGGATTCTATCAA+GGG | - | Chr2:68789734-68789753 | MsG0280010378.01.T01:CDS | 30.0% |
| ! | TGCGGTTAAGTATAAGATTT+TGG | - | Chr2:68789870-68789889 | MsG0280010378.01.T01:CDS | 30.0% |
| !! | AGTGTTGAGAGTATTGTAAA+AGG | + | Chr2:68789692-68789711 | None:intergenic | 30.0% |
| !! | GTTAACAAGTTTTAACGTGA+AGG | + | Chr2:68789620-68789639 | None:intergenic | 30.0% |
| AAAAGCTAAGAAGAATGGAG+TGG | - | Chr2:68789765-68789784 | MsG0280010378.01.T01:CDS | 35.0% | |
| AATGTGGAAATTGGAGATTC+TGG | - | Chr2:68789931-68789950 | MsG0280010378.01.T01:CDS | 35.0% | |
| ATGTGGAAATTGGAGATTCT+GGG | - | Chr2:68789932-68789951 | MsG0280010378.01.T01:CDS | 35.0% | |
| GAATTTAACTGCGAGTGTTA+AGG | - | Chr2:68789804-68789823 | MsG0280010378.01.T01:CDS | 35.0% | |
| GTATGCTTAGCCATATGAAT+AGG | + | Chr2:68789504-68789523 | None:intergenic | 35.0% | |
| TGGTAAAGTTTATCTAAGGG+TGG | - | Chr2:68789837-68789856 | MsG0280010378.01.T01:CDS | 35.0% | |
| TTGGTATACGAAAAGAGATC+GGG | - | Chr2:68789894-68789913 | MsG0280010378.01.T01:CDS | 35.0% | |
| ! | AAATGGGAAAGAAAAGTGTG+AGG | - | Chr2:68789959-68789978 | MsG0280010378.01.T01:CDS | 35.0% |
| ! | TTTTAACGTGAAGGTGATAG+TGG | + | Chr2:68789611-68789630 | None:intergenic | 35.0% |
| !! | TGATCTTGCTGTTGTGTTTA+GGG | + | Chr2:68789584-68789603 | None:intergenic | 35.0% |
| !! | TTGATCTTGCTGTTGTGTTT+AGG | + | Chr2:68789585-68789604 | None:intergenic | 35.0% |
| !!! | AAGGGTGGATTATTTTACTG+CGG | - | Chr2:68789852-68789871 | MsG0280010378.01.T01:CDS | 35.0% |
| !!! | GTGTTTTGGGTCTTGTTTAT+TGG | - | Chr2:68790054-68790073 | MsG0280010378.01.T01:CDS | 35.0% |
| !!! | TGTGTTTAGGGTTTTGTTGA+GGG | + | Chr2:68789572-68789591 | None:intergenic | 35.0% |
| !!! | TTGTGTTTAGGGTTTTGTTG+AGG | + | Chr2:68789573-68789592 | None:intergenic | 35.0% |
| ATACGAAAAGAGATCGGGTA+TGG | - | Chr2:68789899-68789918 | MsG0280010378.01.T01:CDS | 40.0% | |
| TACGAAAAGAGATCGGGTAT+GGG | - | Chr2:68789900-68789919 | MsG0280010378.01.T01:CDS | 40.0% | |
| TGTGGAAATTGGAGATTCTG+GGG | - | Chr2:68789933-68789952 | MsG0280010378.01.T01:CDS | 40.0% | |
| !! | AGTGTTAAGGGTGTTGTTGA+TGG | - | Chr2:68789817-68789836 | MsG0280010378.01.T01:CDS | 40.0% |
| !! | GGGTCTTGTTTATTGGTCTT+GGG | - | Chr2:68790061-68790080 | MsG0280010378.01.T01:CDS | 40.0% |
| !! | TGGGTCTTGTTTATTGGTCT+TGG | - | Chr2:68790060-68790079 | MsG0280010378.01.T01:CDS | 40.0% |
| ACGAAAAGAGATCGGGTATG+GGG | - | Chr2:68789901-68789920 | MsG0280010378.01.T01:CDS | 45.0% | |
| AGCTAAGAAGAATGGAGTGG+TGG | - | Chr2:68789768-68789787 | MsG0280010378.01.T01:CDS | 45.0% | |
| ! | AAGAAAAGTGTGAGGCTTGG+AGG | - | Chr2:68789967-68789986 | MsG0280010378.01.T01:CDS | 45.0% |
| ! | AGAAAAGTGTGAGGCTTGGA+GGG | - | Chr2:68789968-68789987 | MsG0280010378.01.T01:CDS | 45.0% |
| ! | ATCGTGCGCTTCTTGTGTTT+TGG | - | Chr2:68790040-68790059 | MsG0280010378.01.T01:CDS | 45.0% |
| ! | GGAAAGAAAAGTGTGAGGCT+TGG | - | Chr2:68789964-68789983 | MsG0280010378.01.T01:CDS | 45.0% |
| ! | GGAGATTCTGGGGAGAAAAT+GGG | - | Chr2:68789943-68789962 | MsG0280010378.01.T01:CDS | 45.0% |
| ! | TCGTGCGCTTCTTGTGTTTT+GGG | - | Chr2:68790041-68790060 | MsG0280010378.01.T01:CDS | 45.0% |
| ! | TGGAGATTCTGGGGAGAAAA+TGG | - | Chr2:68789942-68789961 | MsG0280010378.01.T01:CDS | 45.0% |
| !!! | TTTAGGGTTTTGTTGAGGGC+AGG | + | Chr2:68789568-68789587 | None:intergenic | 45.0% |
| AAAAGAGATCGGGTATGGGG+AGG | - | Chr2:68789904-68789923 | MsG0280010378.01.T01:CDS | 50.0% | |
| AAAGAGATCGGGTATGGGGA+GGG | - | Chr2:68789905-68789924 | MsG0280010378.01.T01:CDS | 50.0% | |
| GCTTGGAGGGAATGAACCAA+GGG | - | Chr2:68789981-68790000 | MsG0280010378.01.T01:CDS | 50.0% | |
| GGAGGGGCAAATGTGGAAAT+TGG | - | Chr2:68789922-68789941 | MsG0280010378.01.T01:CDS | 50.0% | |
| TCCATACACTTTCCATGCGC+CGG | + | Chr2:68790016-68790035 | None:intergenic | 50.0% | |
| ! | AAGAATGGAGTGGTGGAAGC+TGG | - | Chr2:68789775-68789794 | MsG0280010378.01.T01:CDS | 50.0% |
| ! | GAACCAAGGGTGATAGTGTC+CGG | - | Chr2:68789994-68790013 | MsG0280010378.01.T01:CDS | 50.0% |
| AAGAGATCGGGTATGGGGAG+GGG | - | Chr2:68789906-68789925 | MsG0280010378.01.T01:CDS | 55.0% | |
| GGCTTGGAGGGAATGAACCA+AGG | - | Chr2:68789980-68789999 | MsG0280010378.01.T01:CDS | 55.0% | |
| ! | GGCGCATGGAAAGTGTATGG+AGG | - | Chr2:68790015-68790034 | MsG0280010378.01.T01:CDS | 55.0% |
| ! | TCCGGCGCATGGAAAGTGTA+TGG | - | Chr2:68790012-68790031 | MsG0280010378.01.T01:CDS | 55.0% |
| !!! | GGGTTTTGTTGAGGGCAGGA+AGG | + | Chr2:68789564-68789583 | None:intergenic | 55.0% |
| GGTATGGGGAGGGGCAAATG+TGG | - | Chr2:68789915-68789934 | MsG0280010378.01.T01:CDS | 60.0% | |
| GCGCCGGACACTATCACCCT+TGG | + | Chr2:68790000-68790019 | None:intergenic | 65.0% | |
| ! | GGGTGATAGTGTCCGGCGCA+TGG | - | Chr2:68790001-68790020 | MsG0280010378.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 68789496 | 68790092 | 68789496 | ID=MsG0280010378.01;Name=MsG0280010378.01 |
| Chr2 | mRNA | 68789496 | 68790092 | 68789496 | ID=MsG0280010378.01.T01;Parent=MsG0280010378.01;Name=MsG0280010378.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|198 |
| Chr2 | exon | 68789496 | 68790092 | 68789496 | ID=MsG0280010378.01.T01:exon:14675;Parent=MsG0280010378.01.T01 |
| Chr2 | CDS | 68789496 | 68790092 | 68789496 | ID=MsG0280010378.01.T01:cds;Parent=MsG0280010378.01.T01 |
| Gene Sequence |
| Protein sequence |
