AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180005279.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005279.01.T01 MTR_1g096270 98.936 94 1 0 1 94 828 921 8.01e-58 190
MsG0180005279.01.T01 MTR_1g096260 84.615 91 14 0 4 94 705 795 6.40e-47 159
MsG0180005279.01.T01 MTR_7g091680 46.392 97 45 2 1 90 786 882 7.73e-18 77.0
MsG0180005279.01.T01 MTR_2g070020 42.000 100 52 2 1 94 987 1086 9.58e-16 71.2
MsG0180005279.01.T01 MTR_1g047670 39.604 101 53 2 1 93 989 1089 1.50e-15 70.5
MsG0180005279.01.T01 MTR_2g010470 35.417 96 58 1 2 93 870 965 6.12e-15 68.9
MsG0180005279.01.T01 MTR_7g059285 37.234 94 54 2 1 90 978 1070 2.00e-14 67.4
MsG0180005279.01.T01 MTR_3g090665 40.000 100 52 4 2 94 230 328 2.79e-14 66.6
MsG0180005279.01.T01 MTR_5g096530 40.625 96 50 2 2 90 829 924 5.10e-14 66.2
MsG0180005279.01.T01 MTR_5g045910 38.298 94 52 3 1 90 969 1060 9.44e-14 65.5
MsG0180005279.01.T01 MTR_4g074080 36.458 96 56 1 4 94 546 641 4.27e-13 63.5
MsG0180005279.01.T01 MTR_4g113100 38.542 96 51 3 2 90 520 614 4.74e-13 63.5
MsG0180005279.01.T01 MTR_3g060880 35.922 103 51 3 3 94 957 1055 5.70e-13 63.2
MsG0180005279.01.T01 MTR_2g005810 37.895 95 53 3 1 90 865 958 6.26e-13 63.2
MsG0180005279.01.T01 MTR_1g079520 38.614 101 54 2 1 93 946 1046 6.34e-13 63.2
MsG0180005279.01.T01 MTR_3g093710 37.500 96 52 3 2 90 517 611 6.53e-13 63.2
MsG0180005279.01.T01 MTR_4g036695 37.113 97 54 2 1 90 670 766 6.82e-13 63.2
MsG0180005279.01.T01 MTR_8g099195 39.000 100 53 4 2 94 498 596 7.49e-13 62.8
MsG0180005279.01.T01 MTR_1g097580 35.922 103 57 2 1 94 943 1045 9.09e-13 62.8
MsG0180005279.01.T01 MTR_4g036575 34.653 101 59 2 1 94 899 999 1.09e-12 62.4
MsG0180005279.01.T01 MTR_4g105370 33.663 101 60 2 1 94 951 1051 1.15e-12 62.4
MsG0180005279.01.T01 MTR_8g023720 36.735 98 49 4 4 92 989 1082 2.41e-12 61.6
MsG0180005279.01.T01 MTR_3g090480 37.255 102 56 3 1 94 944 1045 2.55e-12 61.2
MsG0180005279.01.T01 MTR_3g110860 32.292 96 61 1 2 93 867 962 3.47e-12 60.8
MsG0180005279.01.T01 MTR_1g080440 32.000 100 62 2 1 94 888 987 3.65e-12 60.8
MsG0180005279.01.T01 MTR_8g015340 40.000 95 48 4 2 90 776 867 4.37e-12 60.8
MsG0180005279.01.T01 MTR_1g028890 38.776 98 54 3 2 94 461 557 5.22e-12 60.5
MsG0180005279.01.T01 MTR_2g072520 37.500 96 51 4 2 90 202 295 7.97e-12 59.7
MsG0180005279.01.T01 MTR_7g092430 39.773 88 48 1 8 90 779 866 1.05e-11 59.7
MsG0180005279.01.T01 MTR_4g014070 39.080 87 49 2 8 90 685 771 2.23e-11 58.5
MsG0180005279.01.T01 MTR_6g088755 35.789 95 58 2 2 93 865 959 2.42e-11 58.5
MsG0180005279.01.T01 MTR_4g109170 36.000 100 53 3 1 91 985 1082 2.89e-11 58.5
MsG0180005279.01.T01 MTR_2g090710 33.684 95 55 3 1 90 872 963 3.97e-11 58.2
MsG0180005279.01.T01 MTR_5g083480 34.021 97 60 2 2 94 674 770 8.16e-11 57.0
MsG0180005279.01.T01 MTR_5g083480 34.021 97 60 2 2 94 671 767 9.44e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005279.01.T01 AT1G34420 64.516 93 30 1 1 90 871 963 1.98e-31 115
MsG0180005279.01.T01 AT2G41820 48.454 97 43 2 1 90 787 883 3.05e-19 81.3
MsG0180005279.01.T01 AT1G17230 43.269 104 45 3 1 94 977 1076 8.30e-17 74.3
MsG0180005279.01.T01 AT1G17230 43.269 104 45 3 1 94 977 1076 9.60e-17 73.9
MsG0180005279.01.T01 AT3G24240 42.553 94 49 2 1 90 971 1063 9.89e-17 73.9
MsG0180005279.01.T01 AT5G61480 36.634 101 57 2 1 94 901 1001 8.39e-16 71.2
MsG0180005279.01.T01 AT2G26730 41.667 96 48 3 2 90 522 616 6.27e-15 68.9
MsG0180005279.01.T01 AT4G26540 38.000 100 55 2 2 94 943 1042 8.21e-15 68.6
MsG0180005279.01.T01 AT5G63930 37.374 99 56 2 1 93 983 1081 1.40e-14 67.8
MsG0180005279.01.T01 AT5G63930 37.374 99 56 2 1 93 919 1017 1.48e-14 67.8
MsG0180005279.01.T01 AT2G33170 36.000 100 57 3 1 93 1003 1102 2.72e-14 67.0
MsG0180005279.01.T01 AT2G33170 36.000 100 57 3 1 93 1003 1102 2.72e-14 67.0
MsG0180005279.01.T01 AT4G28650 38.614 101 54 3 1 94 881 980 2.85e-14 67.0
MsG0180005279.01.T01 AT1G78530 38.144 97 51 3 2 90 249 344 9.55e-14 65.5
MsG0180005279.01.T01 AT1G10850 37.500 96 55 1 4 94 536 631 1.67e-13 64.7
MsG0180005279.01.T01 AT3G08680 38.000 100 55 3 2 94 516 615 2.02e-13 64.7
MsG0180005279.01.T01 AT3G08680 38.000 100 55 3 2 94 516 615 2.02e-13 64.7
MsG0180005279.01.T01 AT1G25320 41.860 86 43 3 8 86 607 692 6.37e-13 63.2
MsG0180005279.01.T01 AT1G34110 33.663 101 60 2 1 94 953 1053 7.32e-13 63.2
MsG0180005279.01.T01 AT1G34110 33.663 101 60 2 1 94 953 1053 7.61e-13 62.8
MsG0180005279.01.T01 AT5G07280 36.842 95 54 1 2 90 1095 1189 9.75e-13 62.8
MsG0180005279.01.T01 AT1G09970 30.208 96 63 1 2 93 859 954 1.65e-12 62.0
MsG0180005279.01.T01 AT1G09970 30.208 96 63 1 2 93 860 955 1.65e-12 62.0
MsG0180005279.01.T01 AT5G56040 35.354 99 57 2 3 94 943 1041 2.70e-12 61.2
MsG0180005279.01.T01 AT1G48480 35.000 100 58 3 2 94 544 643 3.66e-12 60.8
MsG0180005279.01.T01 AT3G53590 37.079 89 55 1 2 90 803 890 5.42e-12 60.5
MsG0180005279.01.T01 AT5G05160 34.951 103 56 4 2 94 516 617 5.61e-12 60.5
MsG0180005279.01.T01 AT4G21410 36.667 90 49 2 8 90 543 631 2.32e-11 58.5
MsG0180005279.01.T01 AT4G21410 36.667 90 49 2 8 90 418 506 2.77e-11 58.5
MsG0180005279.01.T01 AT3G19700 34.737 95 56 2 2 90 869 963 3.81e-11 58.2
MsG0180005279.01.T01 AT1G68400 34.000 100 56 4 4 94 539 637 3.92e-11 58.2
MsG0180005279.01.T01 AT5G48940 33.684 95 55 3 1 90 972 1063 4.44e-11 57.8
MsG0180005279.01.T01 AT4G20270 32.323 99 62 2 1 94 889 987 4.46e-11 57.8
MsG0180005279.01.T01 AT3G51740 36.264 91 54 2 8 94 719 809 7.55e-11 57.4

Find 16 sgRNAs with CRISPR-Local

Find 13 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTAGCACAGTCTTCATCTT+TGG 0.347248 1:+89564093 None:intergenic
TCTCTAGAATTGCAAGCATT+TGG 0.380643 1:+89564154 None:intergenic
GCAGGGAATGTGTATAGCTT+TGG 0.432117 1:-89564318 MsG0180005279.01.T01:CDS
ATTCTACTAGAGTTGCTAAC+AGG 0.448081 1:-89564291 MsG0180005279.01.T01:CDS
CATCTTTGGTCTTGTATCAG+AGG 0.457562 1:+89564107 None:intergenic
AGTGACTGAGGGAACTGAAT+TGG 0.487593 1:-89564259 MsG0180005279.01.T01:CDS
ACAATGAGAGTAACAATGGC+AGG 0.508110 1:-89564336 MsG0180005279.01.T01:CDS
TCAGTTCCCTCAGTCACTGC+AGG 0.536050 1:+89564264 None:intergenic
GAACGTTAGTAGAACATCAC+AGG 0.570926 1:-89564187 MsG0180005279.01.T01:CDS
AGGCAGACCTGCAGTGACTG+AGG 0.603577 1:-89564271 MsG0180005279.01.T01:CDS
CAATGAGAGTAACAATGGCA+GGG 0.611861 1:-89564335 MsG0180005279.01.T01:CDS
GGGAACTGAATTGGTGAAGT+GGG 0.626819 1:-89564250 MsG0180005279.01.T01:CDS
GGCAGACCTGCAGTGACTGA+GGG 0.627237 1:-89564270 MsG0180005279.01.T01:CDS
CAAAGATGAAGACTGTGCTA+CGG 0.629670 1:-89564092 MsG0180005279.01.T01:CDS
AGGGAACTGAATTGGTGAAG+TGG 0.642045 1:-89564251 MsG0180005279.01.T01:CDS
ATACACAATGAGAGTAACAA+TGG 0.664224 1:-89564340 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ATTCTACTAGAGTTGCTAAC+AGG - Chr1:89564113-89564132 MsG0180005279.01.T01:CDS 35.0%
!! TCTCTAGAATTGCAAGCATT+TGG + Chr1:89564253-89564272 None:intergenic 35.0%
CAAAGATGAAGACTGTGCTA+CGG - Chr1:89564312-89564331 MsG0180005279.01.T01:CDS 40.0%
GAACGTTAGTAGAACATCAC+AGG - Chr1:89564217-89564236 MsG0180005279.01.T01:CDS 40.0%
!! CATCTTTGGTCTTGTATCAG+AGG + Chr1:89564300-89564319 None:intergenic 40.0%
AGGGAACTGAATTGGTGAAG+TGG - Chr1:89564153-89564172 MsG0180005279.01.T01:CDS 45.0%
AGTGACTGAGGGAACTGAAT+TGG - Chr1:89564145-89564164 MsG0180005279.01.T01:CDS 45.0%
CGTAGCACAGTCTTCATCTT+TGG + Chr1:89564314-89564333 None:intergenic 45.0%
GCAGGGAATGTGTATAGCTT+TGG - Chr1:89564086-89564105 MsG0180005279.01.T01:CDS 45.0%
GGGAACTGAATTGGTGAAGT+GGG - Chr1:89564154-89564173 MsG0180005279.01.T01:CDS 45.0%
TCAGTTCCCTCAGTCACTGC+AGG + Chr1:89564143-89564162 None:intergenic 55.0%
AGGCAGACCTGCAGTGACTG+AGG - Chr1:89564133-89564152 MsG0180005279.01.T01:CDS 60.0%
GGCAGACCTGCAGTGACTGA+GGG - Chr1:89564134-89564153 MsG0180005279.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr1 gene 89564071 89564355 89564071 ID=MsG0180005279.01;Name=MsG0180005279.01
Chr1 mRNA 89564071 89564355 89564071 ID=MsG0180005279.01.T01;Parent=MsG0180005279.01;Name=MsG0180005279.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|94
Chr1 exon 89564071 89564355 89564071 ID=MsG0180005279.01.T01:exon:55669;Parent=MsG0180005279.01.T01
Chr1 CDS 89564071 89564355 89564071 ID=MsG0180005279.01.T01:cds;Parent=MsG0180005279.01.T01
Gene Sequence

>MsG0180005279.01.T01

ATGAGAGTAACAATGGCAGGGAATGTGTATAGCTTTGGAGTCATTCTACTAGAGTTGCTAACAGGCAGACCTGCAGTGACTGAGGGAACTGAATTGGTGAAGTGGGTTTTACGTAATTCAAGAAATCATGATATCATTCTTGATTTGAACGTTAGTAGAACATCACAGGCTGTTAGAAACCAAATGCTTGCAATTCTAGAGATTGCTCTTGTTTGTGTTAGCACATCCTCTGATACAAGACCAAAGATGAAGACTGTGCTACGGATGTTGCTGAATGCCAGATAA

Protein sequence

>MsG0180005279.01.T01

MRVTMAGNVYSFGVILLELLTGRPAVTEGTELVKWVLRNSRNHDIILDLNVSRTSQAVRNQMLAILEIALVCVSTSSDTRPKMKTVLRMLLNAR*