Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032896.01.T01 | KEH26284.1 | 95.238 | 126 | 6 | 0 | 1 | 126 | 29 | 154 | 1.05E-72 | 224 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032896.01.T01 | O03982 | 66.197 | 71 | 23 | 1 | 3 | 72 | 1 | 71 | 1.23E-24 | 95.5 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032896.01.T01 | A0A072U910 | 95.238 | 126 | 6 | 0 | 1 | 126 | 29 | 154 | 5.02e-73 | 224 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180005279.01 | MsG0680032896.01 | 0.835312 | 1.806026e-56 | 2.200409e-53 |
| MsG0280011163.01 | MsG0680032896.01 | 0.803909 | 2.783248e-49 | 1.415781e-46 |
| MsG0680032476.01 | MsG0680032896.01 | 0.801927 | 7.146882e-49 | 3.455608e-46 |
| MsG0680032896.01 | MsG0680032899.01 | 0.954679 | 1.716823e-112 | 4.939244e-107 |
| MsG0680032896.01 | MsG0680035445.01 | 0.845238 | 4.599524e-59 | 7.615845e-56 |
| MsG0680032896.01 | MsG0680035454.01 | 0.825363 | 4.883725e-54 | 4.441759e-51 |
| MsG0680032896.01 | MsG0780040389.01 | 0.824567 | 7.527607e-54 | 6.692965e-51 |
| MsG0680032896.01 | MsG0780040679.01 | 0.805126 | 1.551522e-49 | 8.143074e-47 |
| MsG0680032896.01 | MsG0780041475.01 | 0.804158 | 2.471123e-49 | 1.264982e-46 |
| MsG0680032896.01 | MsG0780041777.01 | 0.822023 | 2.956380e-53 | 2.445219e-50 |
| MsG0680032896.01 | MsG0880042434.01 | 0.803400 | 3.550227e-49 | 1.782365e-46 |
| MsG0680032896.01 | MsG0880042656.01 | 0.817419 | 3.330716e-52 | 2.424392e-49 |
| MsG0680032896.01 | MsG0880043584.01 | 0.903524 | 3.173714e-79 | 4.715296e-75 |
| MsG0680032896.01 | MsG0880045185.01 | 0.808261 | 3.378409e-50 | 1.922821e-47 |
| MsG0480022575.01 | MsG0680032896.01 | 0.809605 | 1.742461e-50 | 1.027657e-47 |
| MsG0480023099.01 | MsG0680032896.01 | 0.863311 | 2.726049e-64 | 8.227861e-61 |
| MsG0480023898.01 | MsG0680032896.01 | 0.801255 | 9.813211e-49 | 4.664977e-46 |
| MsG0480023900.01 | MsG0680032896.01 | 0.810827 | 9.499837e-51 | 5.786247e-48 |
| MsG0580024596.01 | MsG0680032896.01 | 0.804896 | 1.733757e-49 | 9.046464e-47 |
| MsG0380015289.01 | MsG0680032896.01 | 0.802551 | 5.316350e-49 | 2.611704e-46 |
| MsG0580028453.01 | MsG0680032896.01 | 0.803685 | 3.098567e-49 | 1.566983e-46 |
| MsG0680030629.01 | MsG0680032896.01 | 0.802981 | 4.333557e-49 | 2.152279e-46 |
| MsG0280010378.01 | MsG0680032896.01 | 0.862629 | 4.427298e-64 | 1.305113e-60 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032896.01.T01 | MTR_6g049280 | 95.238 | 126 | 6 | 0 | 1 | 126 | 29 | 154 | 1.27e-76 | 224 |
| MsG0680032896.01.T01 | MTR_6g049320 | 95.238 | 126 | 6 | 0 | 1 | 126 | 29 | 154 | 1.27e-75 | 221 |
| MsG0680032896.01.T01 | MTR_6g049280 | 93.548 | 124 | 8 | 0 | 3 | 126 | 1 | 124 | 1.65e-73 | 214 |
| MsG0680032896.01.T01 | MTR_6g049320 | 93.548 | 124 | 8 | 0 | 3 | 126 | 1 | 124 | 1.52e-72 | 212 |
| MsG0680032896.01.T01 | MTR_6g051690 | 61.240 | 129 | 43 | 3 | 3 | 126 | 1 | 127 | 2.04e-39 | 129 |
| MsG0680032896.01.T01 | MTR_1g099800 | 57.037 | 135 | 46 | 2 | 3 | 125 | 1 | 135 | 6.14e-38 | 125 |
| MsG0680032896.01.T01 | MTR_7g451200 | 55.556 | 108 | 46 | 1 | 3 | 110 | 1 | 106 | 7.21e-37 | 122 |
| MsG0680032896.01.T01 | MTR_1g099810 | 52.174 | 138 | 51 | 2 | 3 | 125 | 1 | 138 | 3.85e-36 | 120 |
| MsG0680032896.01.T01 | MTR_1173s0010 | 55.140 | 107 | 46 | 1 | 3 | 109 | 1 | 105 | 1.21e-35 | 119 |
| MsG0680032896.01.T01 | MTR_7g451210 | 55.263 | 114 | 46 | 2 | 3 | 113 | 1 | 112 | 3.94e-35 | 117 |
| MsG0680032896.01.T01 | MTR_6g051680 | 77.143 | 70 | 16 | 0 | 3 | 72 | 1 | 70 | 1.26e-34 | 116 |
| MsG0680032896.01.T01 | MTR_3g434890 | 55.140 | 107 | 46 | 1 | 3 | 109 | 1 | 105 | 1.63e-34 | 115 |
| MsG0680032896.01.T01 | MTR_6g049260 | 57.009 | 107 | 44 | 2 | 3 | 109 | 1 | 105 | 3.08e-34 | 115 |
| MsG0680032896.01.T01 | MTR_1g099815 | 54.815 | 135 | 45 | 3 | 3 | 125 | 1 | 131 | 5.13e-34 | 115 |
| MsG0680032896.01.T01 | MTR_3g434960 | 54.206 | 107 | 47 | 1 | 3 | 109 | 1 | 105 | 5.14e-34 | 114 |
| MsG0680032896.01.T01 | MTR_8g098915 | 49.618 | 131 | 55 | 3 | 3 | 126 | 1 | 127 | 6.86e-34 | 114 |
| MsG0680032896.01.T01 | MTR_1g102240 | 51.852 | 135 | 49 | 4 | 3 | 125 | 1 | 131 | 2.63e-32 | 110 |
| MsG0680032896.01.T01 | MTR_1g099640 | 48.507 | 134 | 55 | 3 | 3 | 126 | 1 | 130 | 7.55e-32 | 109 |
| MsG0680032896.01.T01 | MTR_3g435000 | 53.465 | 101 | 45 | 1 | 8 | 108 | 10 | 108 | 4.67e-31 | 107 |
| MsG0680032896.01.T01 | MTR_0795s0020 | 52.985 | 134 | 46 | 3 | 3 | 126 | 1 | 127 | 1.57e-30 | 106 |
| MsG0680032896.01.T01 | MTR_1g099810 | 52.101 | 119 | 42 | 2 | 22 | 125 | 1 | 119 | 1.84e-27 | 98.2 |
| MsG0680032896.01.T01 | MTR_7g079110 | 60.294 | 68 | 27 | 0 | 3 | 70 | 1 | 68 | 3.87e-24 | 91.3 |
| MsG0680032896.01.T01 | MTR_8g098915 | 47.321 | 112 | 48 | 3 | 22 | 126 | 1 | 108 | 1.29e-23 | 88.2 |
| MsG0680032896.01.T01 | MTR_3g434920 | 53.750 | 80 | 35 | 1 | 30 | 109 | 2 | 79 | 1.17e-21 | 82.4 |
| MsG0680032896.01.T01 | MTR_1g099810 | 46.602 | 103 | 40 | 2 | 38 | 125 | 1 | 103 | 5.79e-17 | 71.2 |
| MsG0680032896.01.T01 | MTR_8g028615 | 66.176 | 68 | 23 | 0 | 3 | 70 | 1 | 68 | 1.72e-15 | 68.6 |
| MsG0680032896.01.T01 | MTR_1g099640 | 43.434 | 99 | 42 | 3 | 38 | 126 | 1 | 95 | 1.97e-14 | 64.3 |
| MsG0680032896.01.T01 | MTR_3g082250 | 42.683 | 82 | 43 | 4 | 4 | 81 | 4 | 85 | 2.26e-13 | 62.0 |
| MsG0680032896.01.T01 | MTR_5g020960 | 30.128 | 156 | 77 | 4 | 2 | 125 | 1 | 156 | 1.53e-11 | 58.5 |
| MsG0680032896.01.T01 | MTR_6g045257 | 50.000 | 72 | 35 | 1 | 2 | 72 | 1 | 72 | 5.94e-11 | 55.8 |
| MsG0680032896.01.T01 | MTR_1g100587 | 50.000 | 70 | 34 | 1 | 4 | 72 | 4 | 73 | 6.67e-11 | 55.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032896.01.T01 | AT1G01490 | 66.197 | 71 | 23 | 1 | 3 | 72 | 1 | 71 | 1.26e-25 | 95.5 |
| MsG0680032896.01.T01 | AT1G01490 | 66.197 | 71 | 23 | 1 | 3 | 72 | 1 | 71 | 1.26e-25 | 95.5 |
| MsG0680032896.01.T01 | AT1G01490 | 66.667 | 72 | 23 | 1 | 2 | 72 | 27 | 98 | 4.54e-25 | 94.7 |
| MsG0680032896.01.T01 | AT5G52740 | 56.522 | 69 | 28 | 1 | 3 | 71 | 1 | 67 | 1.45e-18 | 75.5 |
| MsG0680032896.01.T01 | AT5G52760 | 42.056 | 107 | 52 | 4 | 4 | 107 | 4 | 103 | 1.66e-16 | 70.5 |
| MsG0680032896.01.T01 | AT5G52750 | 44.615 | 65 | 36 | 0 | 5 | 69 | 5 | 69 | 1.57e-12 | 60.5 |
| MsG0680032896.01.T01 | AT1G63960 | 38.571 | 70 | 42 | 1 | 3 | 71 | 11 | 80 | 2.28e-12 | 60.5 |
| MsG0680032896.01.T01 | AT5G23760 | 51.389 | 72 | 34 | 1 | 2 | 72 | 1 | 72 | 4.53e-12 | 58.5 |
| MsG0680032896.01.T01 | AT1G63950 | 29.600 | 125 | 78 | 3 | 3 | 126 | 1 | 116 | 7.97e-11 | 55.8 |
Find 22 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGACAGTCTCTGGCCTTTC+AGG | 0.146342 | 6:-52340352 | MsG0680032896.01.T01:intron |
| GACAAGAAATTAACCTTAAT+TGG | 0.151973 | 6:-52339715 | MsG0680032896.01.T01:CDS |
| TTATATTTCATATGATTTAT+TGG | 0.157338 | 6:-52339426 | MsG0680032896.01.T01:three_prime_UTR |
| TGGATCTGTGTCTCCAATTA+AGG | 0.276564 | 6:+52339702 | None:intergenic |
| TTATTGGATTTGTAACATAT+TGG | 0.298794 | 6:-52339410 | MsG0680032896.01.T01:three_prime_UTR |
| ATTTGATTCTTCTTCACATC+AGG | 0.383077 | 6:+52339586 | None:intergenic |
| AGTTTACTTACTACATGTAC+TGG | 0.438622 | 6:+52339682 | None:intergenic |
| GTTACAGAAACTAGTATTGA+AGG | 0.451235 | 6:-52340413 | MsG0680032896.01.T01:CDS |
| TAGTAAGTAAACTAAGGAAA+TGG | 0.475788 | 6:-52339672 | MsG0680032896.01.T01:CDS |
| ATGCTGAAATAGTTTCTGTT+GGG | 0.476513 | 6:-52339645 | MsG0680032896.01.T01:CDS |
| AAAGCTATGAAGACAGTCTC+TGG | 0.487579 | 6:-52340361 | MsG0680032896.01.T01:CDS |
| CATGCTGAAATAGTTTCTGT+TGG | 0.500770 | 6:-52339646 | MsG0680032896.01.T01:CDS |
| TACATGTAGTAAGTAAACTA+AGG | 0.515410 | 6:-52339678 | MsG0680032896.01.T01:CDS |
| CAGCATATGACACATCCAAC+AGG | 0.518380 | 6:+52339475 | None:intergenic |
| ATATTCCTCTGTTTCAAGAG+TGG | 0.533231 | 6:-52339506 | MsG0680032896.01.T01:CDS |
| GAGGAATATTGAATATATTG+TGG | 0.535752 | 6:+52339520 | None:intergenic |
| CATGTAGTAATAATGAGGAT+AGG | 0.591628 | 6:+52339549 | None:intergenic |
| ACATATTGACATTACCTGAA+AGG | 0.607504 | 6:+52340338 | None:intergenic |
| TTGTTCCACTCTTGAAACAG+AGG | 0.611177 | 6:+52339501 | None:intergenic |
| AGAGTGGAACAAGATCCTGT+TGG | 0.648357 | 6:-52339490 | MsG0680032896.01.T01:CDS |
| TGTTGCATGTAGTAATAATG+AGG | 0.650568 | 6:+52339544 | None:intergenic |
| ACAGAAACTAGTATTGAAGG+TGG | 0.658613 | 6:-52340410 | MsG0680032896.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATAATATATTTATCTACA+CGG | - | Chr6:52339844-52339863 | MsG0680032896.01.T01:intron | 10.0% |
| !! | TTATATTTCATATGATTTAT+TGG | - | Chr6:52340304-52340323 | MsG0680032896.01.T01:intron | 10.0% |
| !!! | TTTTGTTAAAAATTGAAAAA+TGG | + | Chr6:52339654-52339673 | None:intergenic | 10.0% |
| !! | AACATATTGGAAAAAATAAA+AGG | - | Chr6:52340333-52340352 | MsG0680032896.01.T01:intron | 15.0% |
| !! | TTAAAATATGTTACATTATC+TGG | + | Chr6:52339510-52339529 | None:intergenic | 15.0% |
| !!! | AATTTTAGCAAAAAACTTAA+AGG | + | Chr6:52339945-52339964 | None:intergenic | 15.0% |
| !!! | TTTCAATTTTTAACAAAAAG+TGG | - | Chr6:52339656-52339675 | MsG0680032896.01.T01:CDS | 15.0% |
| !! | TTAAACTCATGATATAACAT+TGG | - | Chr6:52339915-52339934 | MsG0680032896.01.T01:intron | 20.0% |
| !! | TTATTGGATTTGTAACATAT+TGG | - | Chr6:52340320-52340339 | MsG0680032896.01.T01:intron | 20.0% |
| !!! | GTTTTTTGCTAAAATTGTAT+TGG | - | Chr6:52339949-52339968 | MsG0680032896.01.T01:intron | 20.0% |
| !!! | TTTGTGTTTTTCAGTTTATA+AGG | - | Chr6:52339559-52339578 | MsG0680032896.01.T01:CDS | 20.0% |
| ! | GACAAGAAATTAACCTTAAT+TGG | - | Chr6:52340015-52340034 | MsG0680032896.01.T01:intron | 25.0% |
| ! | GAGGAATATTGAATATATTG+TGG | + | Chr6:52340213-52340232 | None:intergenic | 25.0% |
| ! | TACATGTAGTAAGTAAACTA+AGG | - | Chr6:52340052-52340071 | MsG0680032896.01.T01:intron | 25.0% |
| ! | TAGTAAGTAAACTAAGGAAA+TGG | - | Chr6:52340058-52340077 | MsG0680032896.01.T01:intron | 25.0% |
| ! | TATTGGTATAATATTTGCAG+GGG | - | Chr6:52339966-52339985 | MsG0680032896.01.T01:intron | 25.0% |
| !! | GTATTGGTATAATATTTGCA+GGG | - | Chr6:52339965-52339984 | MsG0680032896.01.T01:intron | 25.0% |
| !! | TGTATTGGTATAATATTTGC+AGG | - | Chr6:52339964-52339983 | MsG0680032896.01.T01:intron | 25.0% |
| ACATATTGACATTACCTGAA+AGG | + | Chr6:52339395-52339414 | None:intergenic | 30.0% | |
| AGTTTACTTACTACATGTAC+TGG | + | Chr6:52340051-52340070 | None:intergenic | 30.0% | |
| ATGCTGAAATAGTTTCTGTT+GGG | - | Chr6:52340085-52340104 | MsG0680032896.01.T01:intron | 30.0% | |
| ATTTGATTCTTCTTCACATC+AGG | + | Chr6:52340147-52340166 | None:intergenic | 30.0% | |
| CATGTAGTAATAATGAGGAT+AGG | + | Chr6:52340184-52340203 | None:intergenic | 30.0% | |
| GCTAAAATGTTCAAATTGTG+AGG | + | Chr6:52339607-52339626 | None:intergenic | 30.0% | |
| TCAGGTAATGTCAATATGTA+CGG | - | Chr6:52339396-52339415 | MsG0680032896.01.T01:three_prime_UTR | 30.0% | |
| TGTTGCATGTAGTAATAATG+AGG | + | Chr6:52340189-52340208 | None:intergenic | 30.0% | |
| ! | GTTACAGAAACTAGTATTGA+AGG | - | Chr6:52339317-52339336 | MsG0680032896.01.T01:three_prime_UTR | 30.0% |
| ! | TTCTTCTTTTCATCTTTTGC+AGG | + | Chr6:52340111-52340130 | None:intergenic | 30.0% |
| !! | GTGTTTTTCAGTTTATAAGG+TGG | - | Chr6:52339562-52339581 | MsG0680032896.01.T01:CDS | 30.0% |
| ATATTCCTCTGTTTCAAGAG+TGG | - | Chr6:52340224-52340243 | MsG0680032896.01.T01:intron | 35.0% | |
| CATGCTGAAATAGTTTCTGT+TGG | - | Chr6:52340084-52340103 | MsG0680032896.01.T01:intron | 35.0% | |
| CGAGGTAGTAAAAAATGTTG+CGG | - | Chr6:52339787-52339806 | MsG0680032896.01.T01:intron | 35.0% | |
| ! | ACAGAAACTAGTATTGAAGG+TGG | - | Chr6:52339320-52339339 | MsG0680032896.01.T01:three_prime_UTR | 35.0% |
| ! | TGTCTTCATAGCTTTTTGCT+TGG | + | Chr6:52339364-52339383 | None:intergenic | 35.0% |
| !!! | CGCAACATTTTTTACTACCT+CGG | + | Chr6:52339789-52339808 | None:intergenic | 35.0% |
| AAAGCTATGAAGACAGTCTC+TGG | - | Chr6:52339369-52339388 | MsG0680032896.01.T01:three_prime_UTR | 40.0% | |
| TGGATCTGTGTCTCCAATTA+AGG | + | Chr6:52340031-52340050 | None:intergenic | 40.0% | |
| TTGTTCCACTCTTGAAACAG+AGG | + | Chr6:52340232-52340251 | None:intergenic | 40.0% | |
| AGAGTGGAACAAGATCCTGT+TGG | - | Chr6:52340240-52340259 | MsG0680032896.01.T01:intron | 45.0% | |
| CAGCATATGACACATCCAAC+AGG | + | Chr6:52340258-52340277 | None:intergenic | 45.0% | |
| AATCCGCGTAAGTGTAACCG+AGG | - | Chr6:52339769-52339788 | MsG0680032896.01.T01:intron | 50.0% | |
| CTACCTCGGTTACACTTACG+CGG | + | Chr6:52339775-52339794 | None:intergenic | 50.0% | |
| ! | AAGACAGTCTCTGGCCTTTC+AGG | - | Chr6:52339378-52339397 | MsG0680032896.01.T01:three_prime_UTR | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 52339312 | 52340440 | 52339312 | ID=MsG0680032896.01;Name=MsG0680032896.01 |
| Chr6 | mRNA | 52339312 | 52340440 | 52339312 | ID=MsG0680032896.01.T01;Parent=MsG0680032896.01;Name=MsG0680032896.01.T01;_AED=0.50;_eAED=0.50;_QI=0|1|0.5|1|1|1|2|162|126 |
| Chr6 | exon | 52340353 | 52340440 | 52340353 | ID=MsG0680032896.01.T01:exon:13415;Parent=MsG0680032896.01.T01 |
| Chr6 | exon | 52339312 | 52339766 | 52339312 | ID=MsG0680032896.01.T01:exon:13416;Parent=MsG0680032896.01.T01 |
| Chr6 | CDS | 52340353 | 52340440 | 52340353 | ID=MsG0680032896.01.T01:cds;Parent=MsG0680032896.01.T01 |
| Chr6 | CDS | 52339474 | 52339766 | 52339474 | ID=MsG0680032896.01.T01:cds;Parent=MsG0680032896.01.T01 |
| Chr6 | three_prime_UTR | 52339312 | 52339473 | 52339312 | ID=MsG0680032896.01.T01:three_prime_utr;Parent=MsG0680032896.01.T01 |
| Gene Sequence |
| Protein sequence |
