Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036260.01.T01 | AFC88126.1 | 57.028 | 249 | 92 | 5 | 150 | 395 | 70 | 306 | 1.23E-92 | 291 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000489.01 | MsG0780036260.01 | -0.802433 | 5.623886e-49 | 2.754585e-46 |
| MsG0180000599.01 | MsG0780036260.01 | -0.830387 | 3.021462e-55 | 3.178699e-52 |
| MsG0180002700.01 | MsG0780036260.01 | -0.829811 | 4.177787e-55 | 4.323432e-52 |
| MsG0180005059.01 | MsG0780036260.01 | 0.864951 | 8.397783e-65 | 2.685029e-61 |
| MsG0180005060.01 | MsG0780036260.01 | 0.827202 | 1.782514e-54 | 1.709126e-51 |
| MsG0180005082.01 | MsG0780036260.01 | 0.876309 | 1.547149e-68 | 7.464777e-65 |
| MsG0180005130.01 | MsG0780036260.01 | 0.882977 | 6.621090e-71 | 4.119940e-67 |
| MsG0180005143.01 | MsG0780036260.01 | 0.879759 | 9.582375e-70 | 5.259371e-66 |
| MsG0180006253.01 | MsG0780036260.01 | 0.830855 | 2.321765e-55 | 2.476352e-52 |
| MsG0280006553.01 | MsG0780036260.01 | -0.810004 | 1.429670e-50 | 8.520857e-48 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036260.01.T01 | MTR_3g118390 | 57.895 | 247 | 90 | 4 | 152 | 395 | 129 | 364 | 1.04e-94 | 289 |
| MsG0780036260.01.T01 | MTR_8g074350 | 49.393 | 247 | 110 | 5 | 152 | 395 | 83 | 317 | 4.35e-78 | 244 |
| MsG0780036260.01.T01 | MTR_8g074330 | 48.133 | 241 | 110 | 5 | 158 | 395 | 89 | 317 | 5.07e-75 | 236 |
| MsG0780036260.01.T01 | MTR_8g074335 | 44.215 | 242 | 107 | 5 | 158 | 396 | 91 | 307 | 1.13e-63 | 207 |
| MsG0780036260.01.T01 | MTR_7g110780 | 40.316 | 253 | 125 | 8 | 152 | 395 | 39 | 274 | 1.11e-57 | 190 |
| MsG0780036260.01.T01 | MTR_2g034680 | 33.617 | 235 | 140 | 5 | 157 | 384 | 64 | 289 | 3.77e-37 | 137 |
| MsG0780036260.01.T01 | MTR_2g099465 | 32.422 | 256 | 128 | 8 | 139 | 391 | 41 | 254 | 1.77e-36 | 134 |
| MsG0780036260.01.T01 | MTR_2g099470 | 33.712 | 264 | 127 | 10 | 131 | 391 | 64 | 282 | 2.44e-35 | 131 |
| MsG0780036260.01.T01 | MTR_1g105535 | 31.837 | 245 | 149 | 7 | 147 | 384 | 62 | 295 | 3.16e-34 | 129 |
| MsG0780036260.01.T01 | MTR_7g021640 | 31.197 | 234 | 144 | 6 | 158 | 384 | 69 | 292 | 4.36e-32 | 124 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036260.01.T01 | AT3G12500 | 52.400 | 250 | 101 | 5 | 152 | 396 | 89 | 325 | 3.12e-86 | 266 |
| MsG0780036260.01.T01 | AT4G01700 | 42.041 | 245 | 121 | 7 | 158 | 395 | 47 | 277 | 3.50e-63 | 204 |
| MsG0780036260.01.T01 | AT1G02360 | 40.149 | 269 | 140 | 6 | 134 | 395 | 15 | 269 | 5.03e-62 | 201 |
| MsG0780036260.01.T01 | AT1G02360 | 40.149 | 269 | 140 | 6 | 134 | 395 | 67 | 321 | 2.46e-61 | 201 |
| MsG0780036260.01.T01 | AT1G02360 | 40.149 | 269 | 140 | 6 | 134 | 395 | 61 | 315 | 2.52e-61 | 201 |
| MsG0780036260.01.T01 | AT2G43590 | 32.129 | 249 | 119 | 9 | 137 | 383 | 56 | 256 | 1.46e-36 | 134 |
| MsG0780036260.01.T01 | AT3G54420 | 33.203 | 256 | 127 | 8 | 138 | 391 | 60 | 273 | 1.92e-35 | 132 |
| MsG0780036260.01.T01 | AT1G05850 | 31.356 | 236 | 145 | 6 | 153 | 381 | 64 | 289 | 7.16e-35 | 131 |
| MsG0780036260.01.T01 | AT1G05850 | 31.356 | 236 | 145 | 6 | 153 | 381 | 64 | 289 | 7.16e-35 | 131 |
| MsG0780036260.01.T01 | AT3G16920 | 31.197 | 234 | 145 | 5 | 158 | 384 | 77 | 301 | 4.15e-34 | 129 |
Find 138 sgRNAs with CRISPR-Local
Find 176 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACTGGAAATGTGGAGGT+GGG | 0.608412 | 7:+5496618 | MsG0780036260.01.T01:CDS |
| AAACCATAAAGCACTCTTGA+AGG | 0.417998 | 7:-5496438 | None:intergenic |
| AACATAGATTACGGAAGATG+TGG | 0.601170 | 7:+5496353 | MsG0780036260.01.T01:CDS |
| AACTGAAGGACCTTACGATT+GGG | 0.431682 | 7:+5496214 | MsG0780036260.01.T01:CDS |
| AAGCAATATCCCCAATCGTA+AGG | 0.585697 | 7:-5496224 | None:intergenic |
| AATCTATGTTTCCAGTTATC+TGG | 0.221981 | 7:-5496340 | None:intergenic |
| ACAAGTGGTGGATCAACTGA+AGG | 0.633335 | 7:+5496200 | MsG0780036260.01.T01:CDS |
| ACCGAACTTGGGGAAGGTTT+TGG | 0.324729 | 7:-5496111 | None:intergenic |
| ACGATGATGATCTGTGTGAG+GGG | 0.740758 | 7:+5496051 | MsG0780036260.01.T01:CDS |
| ACGTCGGATGTCTGAATCGG+AGG | 0.626982 | 7:-5496470 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AATCTATGTTTCCAGTTATC+TGG | - | Chr7:5496343-5496362 | None:intergenic | 30.0% | |
| AGTAGTACTATTACTACTGT+CGG | - | Chr7:5495579-5495598 | None:intergenic | 30.0% | |
| CACTCTTGAAGGATATAATT+GGG | - | Chr7:5496430-5496449 | None:intergenic | 30.0% | |
| TTATAATAGGATCTACAGGA+TGG | + | Chr7:5495601-5495620 | MsG0780036260.01.T01:CDS | 30.0% | |
| AACATAGATTACGGAAGATG+TGG | + | Chr7:5496353-5496372 | MsG0780036260.01.T01:CDS | 35.0% | |
| AGATCAATTGAAAGCCATTG+CGG | + | Chr7:5495684-5495703 | MsG0780036260.01.T01:CDS | 35.0% | |
| ATATTCCAGCCATAACTTCA+TGG | - | Chr7:5496511-5496530 | None:intergenic | 35.0% | |
| ATGAAGTTATGGCTGGAATA+TGG | + | Chr7:5496510-5496529 | MsG0780036260.01.T01:CDS | 35.0% | |
| ATGTTTCCAGTTATCTGGAA+TGG | - | Chr7:5496338-5496357 | None:intergenic | 35.0% | |
| CCTCCCAAAAATTCAAAAAC+TGG | + | Chr7:5496603-5496622 | MsG0780036260.01.T01:CDS | 35.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 5495558 | 5496769 | 5495558 | ID=MsG0780036260.01;Name=MsG0780036260.01 |
| Chr7 | mRNA | 5495558 | 5496769 | 5495558 | ID=MsG0780036260.01.T01;Parent=MsG0780036260.01;Name=MsG0780036260.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|403 |
| Chr7 | exon | 5495558 | 5496769 | 5495558 | ID=MsG0780036260.01.T01:exon:2939;Parent=MsG0780036260.01.T01 |
| Chr7 | CDS | 5495558 | 5496769 | 5495558 | ID=MsG0780036260.01.T01:cds;Parent=MsG0780036260.01.T01 |
| Gene Sequence |
>MsG0780036260.01.T01 ATGACAATCAATCAATTCCCGACAGTAGTAATAGTACTACTAATTATAATAGGATCTACAGGATGGTGCTTGGCGGGTCGAGATAGGGAGGAATGTGGATTATGCTGCAGTGCATGGGATTTTTGTAGATCAATTGAAAGCCATTGCGGCCCTCCCCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTTCTCCCCCTCCCCCTTCTCCTCCACCTCCTTCTCCACCTCCTCCTTCTCCTTCTCCTCCTCCCCCTTCTCCACCTCCTCCTTCTCCTCCACCTCCTTCTCCACCTCCCCCTTCTCCTCCACCTCCTTCTCCCCCTCCTCCTTCTCCTTCTCCCCCTCCTCCACCTCCTTCTCCACCTCCCCCTTCTCCTCCACCTCCTTCTCCCCCTCCTCCTTCTCCTTCTCCCCCTCCCCCTTCTCCTCCCCCTCCTCCTCCTGTGGAGGAGTACCTCATCTCGGTAAAGGATTTCAATGAGATGCTTAAGCATCACGATGATGATCTGTGTGAGGGGAAAGGATTTTACACGTATGAAGCTTTTGTGAAAGCTGCCAAAACCTTCCCCAAGTTCGGTAATGTAGGTACTACAAATTCAAAGAAATCGGACATTGCTGCTTTCTTTAGTCAAAGTTCTCACGAAACAAGTGGTGGATCAACTGAAGGACCTTACGATTGGGGATATTGCTTTGTGAAGGAACCGTATCCGACCAGGGACTACTGCGAACCAAGCTCTGAATTCCCATGTGCTCCCGGGAAGCAATACTACGGTAGGGGTCCATTCCAGATAACTGGAAACATAGATTACGGAAGATGTGGACGTGCAATAGGTGTTGACTTGCTAAAAAATCCAGACCTTGTTGCCACTGACCCAATTATATCCTTCAAGAGTGCTTTATGGTTTTGGATGACGCCTCCGATTCAGACATCCGACGTATATATGCCGGCCTGCCATGAAGTTATGGCTGGAATATGGACTCCTTCTCATGCTGATCAACTTCAGAGAAGGTATGGAGGATACGGGATGACAACTTATGTGGTGACTTCCTCCCAAAAATTCAAAAACTGGAAATGTGGAGGTGGGTTCATGACTCCAAGCGAGATGGATCGGATTGGATTTTACAAGAGATACTGTGACATACTACTTGTTGCATATGGAAACTATGTTGAATGCTATGGCTATCGTTCTAATTCGCTTACTTTCTCTGCCTAG |
| Protein sequence |
>MsG0780036260.01.T01 MTINQFPTVVIVLLIIIGSTGWCLAGRDREECGLCCSAWDFCRSIESHCGPPPPPPPPPPPPSPPPPSPPPPSPPPPSPSPPPPSPPPPSPPPPSPPPPSPPPPSPPPPSPSPPPPPPSPPPPSPPPPSPPPPSPSPPPPSPPPPPPVEEYLISVKDFNEMLKHHDDDLCEGKGFYTYEAFVKAAKTFPKFGNVGTTNSKKSDIAAFFSQSSHETSGGSTEGPYDWGYCFVKEPYPTRDYCEPSSEFPCAPGKQYYGRGPFQITGNIDYGRCGRAIGVDLLKNPDLVATDPIISFKSALWFWMTPPIQTSDVYMPACHEVMAGIWTPSHADQLQRRYGGYGMTTYVVTSSQKFKNWKCGGGFMTPSEMDRIGFYKRYCDILLVAYGNYVECYGYRSNSLTFSA* |
