Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037442.01.T01 | XP_024631910.1 | 90.909 | 88 | 8 | 0 | 10 | 97 | 1027 | 1114 | 2.58E-43 | 160 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037442.01.T01 | A0A2K3MEG5 | 79.545 | 88 | 18 | 0 | 10 | 97 | 82 | 169 | 1.09e-41 | 145 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0680030907.01 | MsG0780037442.01 | 0.808435 | 3.101837e-50 | 1.773471e-47 |
| MsG0680031998.01 | MsG0780037442.01 | 0.816601 | 5.083531e-52 | 3.618185e-49 |
| MsG0480021561.01 | MsG0780037442.01 | 0.916971 | 9.365913e-86 | 2.640022e-81 |
| MsG0480022359.01 | MsG0780037442.01 | 0.815887 | 7.344441e-52 | 5.126030e-49 |
| MsG0480022802.01 | MsG0780037442.01 | 0.815104 | 1.096792e-51 | 7.492427e-49 |
| MsG0580025827.01 | MsG0780037442.01 | 0.820624 | 6.215629e-53 | 4.943147e-50 |
| MsG0580029472.01 | MsG0780037442.01 | 0.820302 | 7.370721e-53 | 5.810015e-50 |
| MsG0680030514.01 | MsG0780037442.01 | 0.802663 | 5.041999e-49 | 2.483940e-46 |
| MsG0680030599.01 | MsG0780037442.01 | 0.801398 | 9.175049e-49 | 4.377094e-46 |
| MsG0280008165.01 | MsG0780037442.01 | 0.801428 | 9.047743e-49 | 4.319686e-46 |
| MsG0280008171.01 | MsG0780037442.01 | 0.801076 | 1.067847e-48 | 5.053506e-46 |
| MsG0780037442.01 | MsG0880043298.01 | 0.830377 | 3.039896e-55 | 3.197158e-52 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 15 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAAGCATACTGTCCTTTAT+TGG | 0.258597 | 7:+26021162 | MsG0780037442.01.T01:CDS |
| ATTAATGGTGCTGCAAAGTT+TGG | 0.320369 | 7:+26020907 | None:intergenic |
| GGAAGTCTTGTTGATTTAGT+TGG | 0.335009 | 7:+26021130 | MsG0780037442.01.T01:CDS |
| GGATGAGGTTACCTTTGAAT+AGG | 0.371797 | 7:+26021183 | MsG0780037442.01.T01:CDS |
| TGGTATGGAACTTCTTTCTC+CGG | 0.445848 | 7:-26021055 | None:intergenic |
| ATTGCAAATGACAATCTTGA+AGG | 0.456999 | 7:+26021109 | MsG0780037442.01.T01:CDS |
| GTTGGCTGTGATTAAGATCA+AGG | 0.469503 | 7:+26020997 | MsG0780037442.01.T01:CDS |
| ATTGTGTGATTGATTCATCT+TGG | 0.474013 | 7:-26021075 | None:intergenic |
| ATACTGTCCTTTATTGGATG+AGG | 0.483836 | 7:+26021168 | MsG0780037442.01.T01:CDS |
| AAGGTAACCTCATCCAATAA+AGG | 0.498890 | 7:-26021175 | None:intergenic |
| GTGATTGATTCATCTTGGTA+TGG | 0.505565 | 7:-26021070 | None:intergenic |
| AATATCTTTATCCTATTCAA+AGG | 0.507351 | 7:-26021194 | None:intergenic |
| TGATTTAGTTGGAGAAGTTG+AGG | 0.526516 | 7:+26021141 | MsG0780037442.01.T01:CDS |
| AACTTTAGAAGATAGTAAGC+CGG | 0.555137 | 7:+26021036 | MsG0780037442.01.T01:CDS |
| TACTTCACACAGTATCCTGA+AGG | 0.615568 | 7:+26020962 | MsG0780037442.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATCCTGAAGGAAAAAAAGAT+TGG | + | Chr7:26020975-26020994 | MsG0780037442.01.T01:CDS | 30.0% | |
| ATTGCAAATGACAATCTTGA+AGG | + | Chr7:26021109-26021128 | MsG0780037442.01.T01:CDS | 30.0% | |
| TGAAGGAAAAAAAGATTGGT+TGG | + | Chr7:26020979-26020998 | MsG0780037442.01.T01:CDS | 30.0% | |
| ! | AACTTTAGAAGATAGTAAGC+CGG | + | Chr7:26021036-26021055 | MsG0780037442.01.T01:CDS | 30.0% |
| ! | ATTGTGTGATTGATTCATCT+TGG | - | Chr7:26021078-26021097 | None:intergenic | 30.0% |
| !! | GTGTGAAGTATACTTGTTTT+TGG | - | Chr7:26020952-26020971 | None:intergenic | 30.0% |
| !!! | AACCAATCTTTTTTTCCTTC+AGG | - | Chr7:26020980-26020999 | None:intergenic | 30.0% |
| AAGGTAACCTCATCCAATAA+AGG | - | Chr7:26021178-26021197 | None:intergenic | 35.0% | |
| ATACTGTCCTTTATTGGATG+AGG | + | Chr7:26021168-26021187 | MsG0780037442.01.T01:CDS | 35.0% | |
| TGATTTAGTTGGAGAAGTTG+AGG | + | Chr7:26021141-26021160 | MsG0780037442.01.T01:CDS | 35.0% | |
| ! | GTGATTGATTCATCTTGGTA+TGG | - | Chr7:26021073-26021092 | None:intergenic | 35.0% |
| !! | GGAAGTCTTGTTGATTTAGT+TGG | + | Chr7:26021130-26021149 | MsG0780037442.01.T01:CDS | 35.0% |
| GTTGGCTGTGATTAAGATCA+AGG | + | Chr7:26020997-26021016 | MsG0780037442.01.T01:CDS | 40.0% | |
| TACTTCACACAGTATCCTGA+AGG | + | Chr7:26020962-26020981 | MsG0780037442.01.T01:CDS | 40.0% | |
| TGGTATGGAACTTCTTTCTC+CGG | - | Chr7:26021058-26021077 | None:intergenic | 40.0% | |
| ! | GGAAGCATACTGTCCTTTAT+TGG | + | Chr7:26021162-26021181 | MsG0780037442.01.T01:CDS | 40.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 26020911 | 26021204 | 26020911 | ID=MsG0780037442.01;Name=MsG0780037442.01 |
| Chr7 | mRNA | 26020911 | 26021204 | 26020911 | ID=MsG0780037442.01.T01;Parent=MsG0780037442.01;Name=MsG0780037442.01.T01;_AED=0.41;_eAED=0.41;_QI=0|-1|0|1|-1|1|1|0|97 |
| Chr7 | exon | 26020911 | 26021204 | 26020911 | ID=MsG0780037442.01.T01:exon:3490;Parent=MsG0780037442.01.T01 |
| Chr7 | CDS | 26020911 | 26021204 | 26020911 | ID=MsG0780037442.01.T01:cds;Parent=MsG0780037442.01.T01 |
| Gene Sequence |
| Protein sequence |
