AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480021561.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 45 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGGCAGGGTTGACGATTC+AGG 0.139753 4:-59846569 MsG0480021561.01.T01:five_prime_UTR
GCGTTGAAAAGTTGCTATTA+TGG 0.273242 4:-59845301 MsG0480021561.01.T01:three_prime_UTR
TGAATTGAAGAGTTTATAAT+AGG 0.289745 4:+59845246 None:intergenic
ATTAATGGTGCTGCAAAGTT+TGG 0.320369 4:-59846478 MsG0480021561.01.T01:exon
GGAAGCATACGGTCCTTTCT+TGG 0.323111 4:-59846223 MsG0480021561.01.T01:CDS
GGAAGTCTTGTTGATTTAGT+TGG 0.335009 4:-59846255 MsG0480021561.01.T01:CDS
CAGTTAGGACCGAGGCTTCT+TGG 0.340085 4:-59846587 None:intergenic
GAGCGCTAGGAGCAAATGTT+TGG 0.365369 4:-59845279 MsG0480021561.01.T01:three_prime_UTR
GAGATGGATGAACTTTAGTT+TGG 0.369334 4:-59845192 MsG0480021561.01.T01:three_prime_UTR
AAAATACATTGAAATCCTTT+GGG 0.375763 4:+59845922 None:intergenic
CAGGAAGGAGAGGGTGATAA+TGG 0.378426 4:-59845222 MsG0480021561.01.T01:three_prime_UTR
TATAAACTCTTCAATTCAAC+AGG 0.378733 4:-59845241 MsG0480021561.01.T01:three_prime_UTR
ATGTAATAGTAGGTGGTCTT+TGG 0.406228 4:-59845323 MsG0480021561.01.T01:three_prime_UTR
TGGATGAGGTTACCTTTGAA+TGG 0.410546 4:-59846203 MsG0480021561.01.T01:CDS
TAGGACCGAGGCTTCTTGGC+AGG 0.415660 4:-59846583 None:intergenic
TTGAATTGAGCTTCTCGCCT+TGG 0.420399 4:+59846371 None:intergenic
TTGGCAGGGTTGACGATTCA+GGG 0.436084 4:-59846568 MsG0480021561.01.T01:five_prime_UTR
TGGTATGGAACTTCTTTCTC+CGG 0.445848 4:+59846330 None:intergenic
GGATGAGGTTACCTTTGAAT+GGG 0.455125 4:-59846202 MsG0480021561.01.T01:CDS
ATTGCAAATGACAATCTTGA+AGG 0.456999 4:-59846276 MsG0480021561.01.T01:CDS
AGAGGTAGAGGCAGCGGAAG+AGG 0.457632 4:-59845993 MsG0480021561.01.T01:three_prime_UTR
ATTGTGTGATTGATTCATCT+TGG 0.474013 4:+59846310 None:intergenic
AATATCTTTATCCCATTCAA+AGG 0.483583 4:+59846191 None:intergenic
TTCAATTCAACAGGAAGGAG+AGG 0.501539 4:-59845232 MsG0480021561.01.T01:three_prime_UTR
ATACGGTCCTTTCTTGGATG+AGG 0.503815 4:-59846217 MsG0480021561.01.T01:CDS
GTGATTGATTCATCTTGGTA+TGG 0.505565 4:+59846315 None:intergenic
AGCGCTAGGAGCAAATGTTT+GGG 0.510625 4:-59845278 MsG0480021561.01.T01:three_prime_UTR
TGATTTAGTTGGAGAAGTTG+AGG 0.526516 4:-59846244 MsG0480021561.01.T01:CDS
CGAATGTTAGGTAGAGGTAG+AGG 0.532296 4:-59846005 MsG0480021561.01.T01:intron
TTAGGTAGAGGTAGAGGCAG+CGG 0.532621 4:-59845999 MsG0480021561.01.T01:intron
AGGACCGAGGCTTCTTGGCA+GGG 0.533687 4:-59846582 None:intergenic
AGTTGCTATTATGGAGCGCT+AGG 0.552952 4:-59845292 MsG0480021561.01.T01:three_prime_UTR
AACTTTAGAAGATAGTAAGC+CGG 0.555137 4:-59846349 MsG0480021561.01.T01:CDS
AAGGTAACCTCATCCAAGAA+AGG 0.563434 4:+59846210 None:intergenic
TCAACCCTGCCAAGAAGCCT+CGG 0.575981 4:+59846578 None:intergenic
TGATAATGGTAACAGCGAGA+TGG 0.587881 4:-59845208 MsG0480021561.01.T01:three_prime_UTR
AACTCTTCAATTCAACAGGA+AGG 0.589557 4:-59845237 MsG0480021561.01.T01:three_prime_UTR
AAAAGAGATGTAATAGTAGG+TGG 0.591998 4:-59845330 MsG0480021561.01.T01:three_prime_UTR
TGATGAAACATATTCTGACT+AGG 0.610583 4:-59846124 MsG0480021561.01.T01:intron
TCAATTCAACAGGAAGGAGA+GGG 0.611912 4:-59845231 MsG0480021561.01.T01:three_prime_UTR
GTTGGCTGTGATTAAGACCA+AGG 0.613774 4:-59846388 MsG0480021561.01.T01:CDS
GGAGAAGTTGAGGAAGCATA+CGG 0.617796 4:-59846234 MsG0480021561.01.T01:CDS
TGGCAGGGTTGACGATTCAG+GGG 0.627660 4:-59846567 MsG0480021561.01.T01:five_prime_UTR
ATCTTCTCAAACAAACCCAA+AGG 0.644202 4:-59845937 MsG0480021561.01.T01:three_prime_UTR
ATTGATGACATAATATCATG+TGG 0.743052 4:-59845076 MsG0480021561.01.T01:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGTTATTTACATGTAAAATA+TGG - Chr4:59845922-59845941 MsG0480021561.01.T01:intron 15.0%
!! TCATATCATATAAAGAAAAT+AGG + Chr4:59845974-59845993 None:intergenic 15.0%
!!! CAATGTATTTTTATGATATA+AGG - Chr4:59845664-59845683 MsG0480021561.01.T01:intron 15.0%
!!! CATTTTGGATAATTTTATAT+TGG - Chr4:59846459-59846478 MsG0480021561.01.T01:CDS 15.0%
!!! TTCATGATTTTTTTTTTTGT+AGG - Chr4:59846198-59846217 MsG0480021561.01.T01:CDS 15.0%
!! AAAAATACATTGAAATCCTT+TGG + Chr4:59845656-59845675 None:intergenic 20.0%
!! AAAATACATTGAAATCCTTT+GGG + Chr4:59845655-59845674 None:intergenic 20.0%
!! AAAATATAATTGTGACATTC+AGG - Chr4:59846578-59846597 MsG0480021561.01.T01:five_prime_UTR 20.0%
!! TGAATTGAAGAGTTTATAAT+AGG + Chr4:59846331-59846350 None:intergenic 20.0%
!! TTAAAAAGAGATGTAATAGT+AGG - Chr4:59846241-59846260 MsG0480021561.01.T01:CDS 20.0%
!!! ATCATGATCATTTTACATTT+TGG - Chr4:59846444-59846463 MsG0480021561.01.T01:CDS 20.0%
!!! TAACTATATTTTGCCATATA+AGG + Chr4:59845907-59845926 None:intergenic 20.0%
! AATATCTTTATCCCATTCAA+AGG + Chr4:59845386-59845405 None:intergenic 25.0%
! AATTTATTATCTGGTAGCAT+AGG - Chr4:59845694-59845713 MsG0480021561.01.T01:intron 25.0%
! ACCATTAATGTAAAAATCCA+GGG + Chr4:59845085-59845104 None:intergenic 25.0%
! ATAGGGAGATATAGTTATTT+AGG - Chr4:59846086-59846105 MsG0480021561.01.T01:intron 25.0%
! ATCCTGAAGTAAAAAAAGAT+TGG - Chr4:59845164-59845183 MsG0480021561.01.T01:three_prime_UTR 25.0%
! CTAAGTAACAACAATATTGA+AGG + Chr4:59846005-59846024 None:intergenic 25.0%
! GAAACAATTGTTACAAATAG+TGG - Chr4:59846052-59846071 MsG0480021561.01.T01:intron 25.0%
! GGTGCTTAAAATTTATTATC+TGG - Chr4:59845685-59845704 MsG0480021561.01.T01:intron 25.0%
! TAGGGAGATATAGTTATTTA+GGG - Chr4:59846087-59846106 MsG0480021561.01.T01:intron 25.0%
! TATAAACTCTTCAATTCAAC+AGG - Chr4:59846333-59846352 MsG0480021561.01.T01:CDS 25.0%
! TGAAGTAAAAAAAGATTGGT+TGG - Chr4:59845168-59845187 MsG0480021561.01.T01:three_prime_UTR 25.0%
!! ATTGATGACATAATATCATG+TGG - Chr4:59846498-59846517 MsG0480021561.01.T01:five_prime_UTR 25.0%
!! CTTCAATATTGTTGTTACTT+AGG - Chr4:59846003-59846022 MsG0480021561.01.T01:intron 25.0%
!! TATATTTTGCCATATAAGGT+AGG + Chr4:59845903-59845922 None:intergenic 25.0%
!!! AACCAATCTTTTTTTACTTC+AGG + Chr4:59845169-59845188 None:intergenic 25.0%
!!! GTGTTTTTGACTTTGTTTAA+TGG - Chr4:59845838-59845857 MsG0480021561.01.T01:intron 25.0%
!!! TTTTTTGTAGGCTAAATTGA+TGG - Chr4:59846210-59846229 MsG0480021561.01.T01:CDS 25.0%
AAAAGAGATGTAATAGTAGG+TGG - Chr4:59846244-59846263 MsG0480021561.01.T01:CDS 30.0%
AGGGAGATATAGTTATTTAG+GGG - Chr4:59846088-59846107 MsG0480021561.01.T01:intron 30.0%
ATAAGTCATGCAAGAATAAG+AGG + Chr4:59845496-59845515 None:intergenic 30.0%
ATAGTGGCAGTTACATAAAT+AGG - Chr4:59846068-59846087 MsG0480021561.01.T01:intron 30.0%
ATCATCATCATCATCATCAT+CGG + Chr4:59845431-59845450 None:intergenic 30.0%
ATTGCAAATGACAATCTTGA+AGG - Chr4:59845298-59845317 MsG0480021561.01.T01:three_prime_UTR 30.0%
CACCATTAATGTAAAAATCC+AGG + Chr4:59845086-59845105 None:intergenic 30.0%
TAAGTCATGCAAGAATAAGA+GGG + Chr4:59845495-59845514 None:intergenic 30.0%
TAGTGGCAGTTACATAAATA+GGG - Chr4:59846069-59846088 MsG0480021561.01.T01:intron 30.0%
TGAAAAGTTGAAATGGCAAA+TGG - Chr4:59846027-59846046 MsG0480021561.01.T01:intron 30.0%
TGATGAAACATATTCTGACT+AGG - Chr4:59845450-59845469 MsG0480021561.01.T01:intron 30.0%
TTAATGTAAAAATCCAGGGT+TGG + Chr4:59845081-59845100 None:intergenic 30.0%
TTGTATTGTTCACGAATGTT+AGG - Chr4:59845557-59845576 MsG0480021561.01.T01:intron 30.0%
! AACTTTAGAAGATAGTAAGC+CGG - Chr4:59845225-59845244 MsG0480021561.01.T01:three_prime_UTR 30.0%
! ACCCTGGATTTTTACATTAA+TGG - Chr4:59845081-59845100 MsG0480021561.01.T01:three_prime_UTR 30.0%
! ATCAAGCTTCATTGTTACTT+AGG - Chr4:59846141-59846160 MsG0480021561.01.T01:CDS 30.0%
! ATTGTGTGATTGATTCATCT+TGG + Chr4:59845267-59845286 None:intergenic 30.0%
!! AGCACTTTTTGTTTCAGTTT+TGG - Chr4:59845745-59845764 MsG0480021561.01.T01:intron 30.0%
!! CTTAGGTTGAAAAGTTGAAA+TGG - Chr4:59846020-59846039 MsG0480021561.01.T01:intron 30.0%
!! GTGTGATGTATACTTGTTTT+TGG + Chr4:59845141-59845160 None:intergenic 30.0%
!!! GTTTTATTTTATACCAACCC+TGG - Chr4:59845065-59845084 MsG0480021561.01.T01:three_prime_UTR 30.0%
AACTCTTCAATTCAACAGGA+AGG - Chr4:59846337-59846356 MsG0480021561.01.T01:CDS 35.0%
ATCTTCTCAAACAAACCCAA+AGG - Chr4:59845637-59845656 MsG0480021561.01.T01:intron 35.0%
ATGTAATAGTAGGTGGTCTT+TGG - Chr4:59846251-59846270 MsG0480021561.01.T01:CDS 35.0%
GAGATGGATGAACTTTAGTT+TGG - Chr4:59846382-59846401 MsG0480021561.01.T01:CDS 35.0%
TGATTTAGTTGGAGAAGTTG+AGG - Chr4:59845330-59845349 MsG0480021561.01.T01:three_prime_UTR 35.0%
! ATTTTGCCATATAAGGTAGG+CGG + Chr4:59845900-59845919 None:intergenic 35.0%
! GCGTTGAAAAGTTGCTATTA+TGG - Chr4:59846273-59846292 MsG0480021561.01.T01:CDS 35.0%
! GTGATTGATTCATCTTGGTA+TGG + Chr4:59845262-59845281 None:intergenic 35.0%
!! AATGGTTGTTTGTTGTGTTG+CGG - Chr4:59845856-59845875 MsG0480021561.01.T01:intron 35.0%
!! ATTAATGGTGCTGCAAAGTT+TGG - Chr4:59845096-59845115 MsG0480021561.01.T01:three_prime_UTR 35.0%
!! GGAAGTCTTGTTGATTTAGT+TGG - Chr4:59845319-59845338 MsG0480021561.01.T01:three_prime_UTR 35.0%
AAGGTAACCTCATCCAAGAA+AGG + Chr4:59845367-59845386 None:intergenic 40.0%
CAGAGAGAAACAATTGCAAC+TGG + Chr4:59845949-59845968 None:intergenic 40.0%
GGATGAGGTTACCTTTGAAT+GGG - Chr4:59845372-59845391 MsG0480021561.01.T01:intron 40.0%
TCAATTCAACAGGAAGGAGA+GGG - Chr4:59846343-59846362 MsG0480021561.01.T01:CDS 40.0%
TGGATGAGGTTACCTTTGAA+TGG - Chr4:59845371-59845390 MsG0480021561.01.T01:intron 40.0%
TGGTATGGAACTTCTTTCTC+CGG + Chr4:59845247-59845266 None:intergenic 40.0%
TGTTCACGAATGTTAGGTAG+AGG - Chr4:59845563-59845582 MsG0480021561.01.T01:intron 40.0%
TTCAATTCAACAGGAAGGAG+AGG - Chr4:59846342-59846361 MsG0480021561.01.T01:CDS 40.0%
!! TGATAATGGTAACAGCGAGA+TGG - Chr4:59846366-59846385 MsG0480021561.01.T01:CDS 40.0%
AGCGCTAGGAGCAAATGTTT+GGG - Chr4:59846296-59846315 MsG0480021561.01.T01:CDS 45.0%
ATACGGTCCTTTCTTGGATG+AGG - Chr4:59845357-59845376 MsG0480021561.01.T01:three_prime_UTR 45.0%
CGAATGTTAGGTAGAGGTAG+AGG - Chr4:59845569-59845588 MsG0480021561.01.T01:intron 45.0%
GTTGGCTGTGATTAAGACCA+AGG - Chr4:59845186-59845205 MsG0480021561.01.T01:three_prime_UTR 45.0%
GTTTCGTGCGGTTGTTTAGA+GGG - Chr4:59845716-59845735 MsG0480021561.01.T01:intron 45.0%
TTGAATTGAGCTTCTCGCCT+TGG + Chr4:59845206-59845225 None:intergenic 45.0%
! AGTTGCTATTATGGAGCGCT+AGG - Chr4:59846282-59846301 MsG0480021561.01.T01:CDS 45.0%
! GGAGAAGTTGAGGAAGCATA+CGG - Chr4:59845340-59845359 MsG0480021561.01.T01:three_prime_UTR 45.0%
! TTTTGGCCGCCTACCTTATA+TGG - Chr4:59845891-59845910 MsG0480021561.01.T01:intron 45.0%
!!! TGCGGCCAGTTTGTTGTTTT+TGG - Chr4:59845874-59845893 MsG0480021561.01.T01:intron 45.0%
CAGGAAGGAGAGGGTGATAA+TGG - Chr4:59846352-59846371 MsG0480021561.01.T01:CDS 50.0%
CTGGTAGCATAGGTTTCGTG+CGG - Chr4:59845704-59845723 MsG0480021561.01.T01:intron 50.0%
GAGCGCTAGGAGCAAATGTT+TGG - Chr4:59846295-59846314 MsG0480021561.01.T01:CDS 50.0%
GGCGGCCAAAAACAACAAAC+TGG + Chr4:59845882-59845901 None:intergenic 50.0%
GGTTTCGTGCGGTTGTTTAG+AGG - Chr4:59845715-59845734 MsG0480021561.01.T01:intron 50.0%
TTAGGTAGAGGTAGAGGCAG+CGG - Chr4:59845575-59845594 MsG0480021561.01.T01:intron 50.0%
TTGGCAGGGTTGACGATTCA+GGG - Chr4:59845006-59845025 MsG0480021561.01.T01:three_prime_UTR 50.0%
! GGAAGCATACGGTCCTTTCT+TGG - Chr4:59845351-59845370 MsG0480021561.01.T01:three_prime_UTR 50.0%
CTTGGCAGGGTTGACGATTC+AGG - Chr4:59845005-59845024 MsG0480021561.01.T01:three_prime_UTR 55.0%
GTGCGGTTGTTTAGAGGGTG+TGG - Chr4:59845721-59845740 MsG0480021561.01.T01:intron 55.0%
TGGCAGGGTTGACGATTCAG+GGG - Chr4:59845007-59845026 MsG0480021561.01.T01:three_prime_UTR 55.0%
AGAGGTAGAGGCAGCGGAAG+AGG - Chr4:59845581-59845600 MsG0480021561.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 59844999 59846597 59844999 ID=MsG0480021561.01;Name=MsG0480021561.01
Chr4 mRNA 59844999 59846597 59844999 ID=MsG0480021561.01.T01;Parent=MsG0480021561.01;Name=MsG0480021561.01.T01;_AED=0.21;_eAED=0.21;_QI=101|1|1|1|0|0.33|3|467|123
Chr4 exon 59846125 59846597 59846125 ID=MsG0480021561.01.T01:exon:21495;Parent=MsG0480021561.01.T01
Chr4 exon 59845929 59846017 59845929 ID=MsG0480021561.01.T01:exon:21496;Parent=MsG0480021561.01.T01
Chr4 exon 59844999 59845376 59844999 ID=MsG0480021561.01.T01:exon:21497;Parent=MsG0480021561.01.T01
Chr4 five_prime_UTR 59846497 59846597 59846497 ID=MsG0480021561.01.T01:five_prime_utr;Parent=MsG0480021561.01.T01
Chr4 CDS 59846125 59846496 59846125 ID=MsG0480021561.01.T01:cds;Parent=MsG0480021561.01.T01
Chr4 three_prime_UTR 59845929 59846017 59845929 ID=MsG0480021561.01.T01:three_prime_utr;Parent=MsG0480021561.01.T01
Chr4 three_prime_UTR 59844999 59845376 59844999 ID=MsG0480021561.01.T01:three_prime_utr;Parent=MsG0480021561.01.T01
Gene Sequence

>MsG0480021561.01.T01

ATGGTGCTGCAAAGTTTGGTTAATGATTTTTGTAAGATCCAAAAACAAGTATACATCACACAGTATCCTGAAGTAAAAAAAGATTGGTTGGCTGTGATTAAGACCAAGGCGAGAAGCTCAATTCAAACTTTAGAAGATAGTAAGCCGGAGAAAGAAGTTCCATACCAAGATGAATCAATCACACAATCACACGTGACAATTGCAAATGACAATCTTGAAGGAAGTCTTGTTGATTTAGTTGGAGAAGTTGAGGAAGCATACGGTCCTTTCTTGGATGAGGTTACCTTTGAATGGGATAAAGATATTGAAGTAGAAGTAGAAGACAGTGCCGATGATGATGATGATGATGATGATGAAACATATTCTGACTAG

Protein sequence

>MsG0480021561.01.T01

MVLQSLVNDFCKIQKQVYITQYPEVKKDWLAVIKTKARSSIQTLEDSKPEKEVPYQDESITQSHVTIANDNLEGSLVDLVGEVEEAYGPFLDEVTFEWDKDIEVEVEDSADDDDDDDDETYSD*