AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022359.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022359.01.T01 MTR_4g088555 95.146 103 4 1 1 102 1 103 2.25e-65 194
MsG0480022359.01.T01 MTR_2g014200 63.380 71 23 2 33 102 36 104 2.56e-23 88.2
MsG0480022359.01.T01 MTR_8g463140 65.909 44 15 0 59 102 62 105 7.69e-18 75.1
MsG0480022359.01.T01 MTR_8g005960 47.727 88 38 3 27 112 155 236 1.27e-15 71.6
MsG0480022359.01.T01 MTR_2g078770 50.877 57 28 0 46 102 42 98 1.80e-13 63.5
MsG0480022359.01.T01 MTR_7g092930 59.091 44 18 0 59 102 65 108 3.46e-13 64.7
MsG0480022359.01.T01 MTR_3g099080 45.588 68 33 1 39 102 71 138 1.39e-12 63.2
MsG0480022359.01.T01 MTR_1g053715 57.447 47 20 0 56 102 68 114 1.88e-12 62.8
MsG0480022359.01.T01 MTR_3g085180 42.373 59 34 0 44 102 147 205 4.54e-12 61.6
MsG0480022359.01.T01 MTR_7g110320 46.970 66 34 1 47 112 130 194 1.18e-11 60.8
MsG0480022359.01.T01 MTR_7g444860 60.976 41 16 0 62 102 149 189 1.18e-11 60.5
MsG0480022359.01.T01 MTR_7g110320 46.970 66 34 1 47 112 130 194 1.42e-11 60.5
MsG0480022359.01.T01 MTR_2g046550 46.667 60 28 1 47 102 120 179 1.72e-11 60.1
MsG0480022359.01.T01 MTR_5g046670 44.643 56 28 1 50 102 229 284 2.40e-11 59.7
MsG0480022359.01.T01 MTR_1g086250 49.091 55 28 0 48 102 133 187 2.58e-11 59.7
MsG0480022359.01.T01 MTR_8g080680 43.902 82 38 2 29 102 91 172 3.04e-11 59.3
MsG0480022359.01.T01 MTR_7g028740 46.429 56 28 1 47 102 64 117 4.06e-11 58.9
MsG0480022359.01.T01 MTR_1g035010 49.123 57 29 0 46 102 88 144 4.11e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022359.01.T01 AT2G33810 57.627 59 22 1 44 102 39 94 3.04e-17 72.4
MsG0480022359.01.T01 AT1G02065 44.048 84 44 2 40 121 164 246 2.79e-14 67.0
MsG0480022359.01.T01 AT1G02065 48.000 75 36 2 40 112 164 237 3.64e-14 67.4
MsG0480022359.01.T01 AT1G53160 54.386 57 23 1 46 102 41 94 1.02e-13 64.3
MsG0480022359.01.T01 AT1G53160 54.386 57 23 1 46 102 41 94 1.02e-13 64.3
MsG0480022359.01.T01 AT1G53160 54.386 57 23 1 46 102 41 94 1.29e-13 63.9
MsG0480022359.01.T01 AT3G60030 50.000 56 28 0 47 102 112 167 2.66e-13 65.5
MsG0480022359.01.T01 AT1G27360 52.830 53 25 0 50 102 163 215 9.99e-13 63.5
MsG0480022359.01.T01 AT1G27360 52.830 53 25 0 50 102 163 215 9.99e-13 63.5
MsG0480022359.01.T01 AT1G27360 52.830 53 25 0 50 102 163 215 9.99e-13 63.5
MsG0480022359.01.T01 AT1G27360 52.830 53 25 0 50 102 163 215 9.99e-13 63.5
MsG0480022359.01.T01 AT2G47070 56.863 51 21 1 62 112 106 155 1.18e-12 63.5
MsG0480022359.01.T01 AT1G27370 39.683 63 38 0 40 102 154 216 1.50e-12 63.2
MsG0480022359.01.T01 AT1G27370 43.860 57 32 0 46 102 160 216 1.60e-12 63.2
MsG0480022359.01.T01 AT1G27370 43.860 57 32 0 46 102 160 216 1.60e-12 63.2
MsG0480022359.01.T01 AT1G27370 43.860 57 32 0 46 102 160 216 1.60e-12 63.2
MsG0480022359.01.T01 AT1G27370 43.860 57 32 0 46 102 160 216 1.60e-12 63.2
MsG0480022359.01.T01 AT1G27370 43.860 57 32 0 46 102 160 216 1.60e-12 63.2
MsG0480022359.01.T01 AT1G27370 43.860 57 32 0 46 102 160 216 1.60e-12 63.2
MsG0480022359.01.T01 AT5G43270 41.250 80 42 1 28 102 130 209 5.51e-12 61.6
MsG0480022359.01.T01 AT5G43270 41.250 80 42 1 28 102 130 209 5.51e-12 61.6
MsG0480022359.01.T01 AT5G43270 41.250 80 42 1 28 102 130 209 5.51e-12 61.6
MsG0480022359.01.T01 AT3G15270 58.537 41 17 0 62 102 63 103 6.46e-12 59.7
MsG0480022359.01.T01 AT1G69170 50.000 56 25 1 50 102 109 164 9.40e-12 60.8
MsG0480022359.01.T01 AT5G50670 48.936 47 24 0 56 102 95 141 4.37e-11 58.9
MsG0480022359.01.T01 AT5G50670 48.936 47 24 0 56 102 95 141 4.37e-11 58.9
MsG0480022359.01.T01 AT5G50570 48.936 47 24 0 56 102 95 141 4.37e-11 58.9
MsG0480022359.01.T01 AT5G50570 48.936 47 24 0 56 102 95 141 4.37e-11 58.9
MsG0480022359.01.T01 AT1G20980 52.830 53 24 1 60 112 118 169 4.62e-11 58.9

Find 26 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAGGAAGAGGATGGTAGA+AGG 0.346008 4:-71556797 MsG0480022359.01.T01:CDS
TGAGGAGGAAGAAGAGGAAG+AGG 0.391649 4:-71556832 MsG0480022359.01.T01:CDS
GCACCTGCCGTACACATTAC+AGG 0.392224 4:-71556618 MsG0480022359.01.T01:CDS
GGAAGAAGAGGAAGAGGATA+CGG 0.438830 4:-71556826 MsG0480022359.01.T01:CDS
AAGAGGATGGTAGAAGGAAG+AGG 0.452598 4:-71556791 MsG0480022359.01.T01:CDS
ATGTGAAATATTCTTGTTGA+GGG 0.453314 4:-71556469 MsG0480022359.01.T01:CDS
GCAATATCACCGGAGGCATA+AGG 0.462023 4:-71556661 MsG0480022359.01.T01:CDS
GGATCATGAGGAGGAAGAAG+AGG 0.499097 4:-71556838 MsG0480022359.01.T01:CDS
AGAGGATGGTAGAAGGAAGA+GGG 0.504666 4:-71556790 MsG0480022359.01.T01:CDS
AATGTGTACGGCAGGTGCTT+TGG 0.505419 4:+71556623 None:intergenic
ACATTACAGGATTGCAGCAA+AGG 0.512816 4:-71556605 MsG0480022359.01.T01:intron
TATTCCACCTTGTTGTCAAG+TGG 0.526109 4:-71556715 MsG0480022359.01.T01:CDS
TTGAAACTATGGAGCCAAGA+AGG 0.558429 4:-71556893 None:intergenic
ACTCACAGACCTTATGCCTC+CGG 0.564755 4:+71556652 None:intergenic
CAATTCTCCACTTGACAACA+AGG 0.571704 4:+71556708 None:intergenic
AATAAGTTTGAAGTACAAAG+AGG 0.596272 4:-71556859 MsG0480022359.01.T01:CDS
GATGTGAAATATTCTTGTTG+AGG 0.596883 4:-71556470 MsG0480022359.01.T01:CDS
CTGCAATCCTGTAATGTGTA+CGG 0.600361 4:+71556611 None:intergenic
TATGGAGCCAAGAAGGTCAG+AGG 0.627805 4:-71556886 MsG0480022359.01.T01:CDS
AATCCTGTAATGTGTACGGC+AGG 0.629458 4:+71556615 None:intergenic
ATGGAGCCAAGAAGGTCAGA+GGG 0.629985 4:-71556885 MsG0480022359.01.T01:CDS
GTACAAAGAGGATCATGAGG+AGG 0.636625 4:-71556847 MsG0480022359.01.T01:CDS
GTGAAGCTAAGCAATATCAC+CGG 0.638561 4:-71556671 MsG0480022359.01.T01:CDS
GAAGTACAAAGAGGATCATG+AGG 0.643502 4:-71556850 MsG0480022359.01.T01:CDS
AAGCTAAGCAATATCACCGG+AGG 0.689023 4:-71556668 MsG0480022359.01.T01:CDS
TTCTCCACTTGACAACAAGG+TGG 0.724295 4:+71556711 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTTACTTAAAAGAGATT+AGG + Chr4:71556781-71556800 None:intergenic 15.0%
!! AATTTACTTAAAAGAGATTA+GGG + Chr4:71556780-71556799 None:intergenic 15.0%
! ATGTGAAATATTCTTGTTGA+GGG - Chr4:71556856-71556875 MsG0480022359.01.T01:CDS 25.0%
!! AATAAGTTTGAAGTACAAAG+AGG - Chr4:71556466-71556485 MsG0480022359.01.T01:CDS 25.0%
GATGTGAAATATTCTTGTTG+AGG - Chr4:71556855-71556874 MsG0480022359.01.T01:CDS 30.0%
AAGAGAAGTTCCAAAAAAGC+TGG - Chr4:71556578-71556597 MsG0480022359.01.T01:intron 35.0%
!!! ATTTGAGCTTCCAGCTTTTT+TGG + Chr4:71556591-71556610 None:intergenic 35.0%
ACATTACAGGATTGCAGCAA+AGG - Chr4:71556720-71556739 MsG0480022359.01.T01:CDS 40.0%
CAATTCTCCACTTGACAACA+AGG + Chr4:71556620-71556639 None:intergenic 40.0%
CTGCAATCCTGTAATGTGTA+CGG + Chr4:71556717-71556736 None:intergenic 40.0%
GAAGTACAAAGAGGATCATG+AGG - Chr4:71556475-71556494 MsG0480022359.01.T01:CDS 40.0%
GTGAAGCTAAGCAATATCAC+CGG - Chr4:71556654-71556673 MsG0480022359.01.T01:CDS 40.0%
! ATTTTTCCCTCTGACCTTCT+TGG + Chr4:71556449-71556468 None:intergenic 40.0%
! TATTCCACCTTGTTGTCAAG+TGG - Chr4:71556610-71556629 MsG0480022359.01.T01:CDS 40.0%
!! AGAGGATACGGATTTTGAAG+AGG - Chr4:71556511-71556530 MsG0480022359.01.T01:intron 40.0%
!! GATTTTGAAGAGGAAGAGGA+TGG - Chr4:71556521-71556540 MsG0480022359.01.T01:intron 40.0%
!! TACGGATTTTGAAGAGGAAG+AGG - Chr4:71556517-71556536 MsG0480022359.01.T01:intron 40.0%
AAGAGGAAGAGGATGGTAGA+AGG - Chr4:71556528-71556547 MsG0480022359.01.T01:intron 45.0%
AAGAGGATGGTAGAAGGAAG+AGG - Chr4:71556534-71556553 MsG0480022359.01.T01:intron 45.0%
AAGCTAAGCAATATCACCGG+AGG - Chr4:71556657-71556676 MsG0480022359.01.T01:CDS 45.0%
AATCCTGTAATGTGTACGGC+AGG + Chr4:71556713-71556732 None:intergenic 45.0%
AGAGGATGGTAGAAGGAAGA+GGG - Chr4:71556535-71556554 MsG0480022359.01.T01:intron 45.0%
GGAAGAAGAGGAAGAGGATA+CGG - Chr4:71556499-71556518 MsG0480022359.01.T01:intron 45.0%
GTACAAAGAGGATCATGAGG+AGG - Chr4:71556478-71556497 MsG0480022359.01.T01:CDS 45.0%
TTCTCCACTTGACAACAAGG+TGG + Chr4:71556617-71556636 None:intergenic 45.0%
!! GGTTTTGTCAGCAATGTAGC+AGG - Chr4:71556741-71556760 MsG0480022359.01.T01:CDS 45.0%
!!! TATATATTTTTGTTTAATTA+AGG - Chr4:71556819-71556838 MsG0480022359.01.T01:CDS 5.0%
AATGTGTACGGCAGGTGCTT+TGG + Chr4:71556705-71556724 None:intergenic 50.0%
ACTCACAGACCTTATGCCTC+CGG + Chr4:71556676-71556695 None:intergenic 50.0%
ATGGAGCCAAGAAGGTCAGA+GGG - Chr4:71556440-71556459 MsG0480022359.01.T01:CDS 50.0%
GGATCATGAGGAGGAAGAAG+AGG - Chr4:71556487-71556506 MsG0480022359.01.T01:CDS 50.0%
TGAGGAGGAAGAAGAGGAAG+AGG - Chr4:71556493-71556512 MsG0480022359.01.T01:intron 50.0%
! GCAATATCACCGGAGGCATA+AGG - Chr4:71556664-71556683 MsG0480022359.01.T01:CDS 50.0%
GCACCTGCCGTACACATTAC+AGG - Chr4:71556707-71556726 MsG0480022359.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr4 gene 71556440 71556907 71556440 ID=MsG0480022359.01;Name=MsG0480022359.01
Chr4 mRNA 71556440 71556907 71556440 ID=MsG0480022359.01.T01;Parent=MsG0480022359.01;Name=MsG0480022359.01.T01;_AED=0.50;_eAED=0.59;_QI=0|0|0|1|1|1|2|0|122
Chr4 exon 71556606 71556907 71556606 ID=MsG0480022359.01.T01:exon:4874;Parent=MsG0480022359.01.T01
Chr4 exon 71556440 71556506 71556440 ID=MsG0480022359.01.T01:exon:4873;Parent=MsG0480022359.01.T01
Chr4 CDS 71556606 71556907 71556606 ID=MsG0480022359.01.T01:cds;Parent=MsG0480022359.01.T01
Chr4 CDS 71556440 71556506 71556440 ID=MsG0480022359.01.T01:cds;Parent=MsG0480022359.01.T01
Gene Sequence

>MsG0480022359.01.T01

ATGGAGCCAAGAAGGTCAGAGGGAAAAATAAGTTTGAAGTACAAAGAGGATCATGAGGAGGAAGAAGAGGAAGAGGATACGGATTTTGAAGAGGAAGAGGATGGTAGAAGGAAGAGGGTAGTGACAGATCTCTATAGCAAGAGAAGTTCCAAAAAAGCTGGAAGCTCAAATATTCCACCTTGTTGTCAAGTGGAGAATTGTGATGCTGATCTTAGTGAAGCTAAGCAATATCACCGGAGGCATAAGGTCTGTGAGTATCATGCCAAAGCACCTGCCGTACACATTACAGGATTGCAGCAAAGGTTAATCTTTGCTAGATGTGAAATATTCTTGTTGAGGGATTTGATTACTGTTTACACTTTTGTCTAA

Protein sequence

>MsG0480022359.01.T01

MEPRRSEGKISLKYKEDHEEEEEEEDTDFEEEEDGRRKRVVTDLYSKRSSKKAGSSNIPPCCQVENCDADLSEAKQYHRRHKVCEYHAKAPAVHITGLQQRLIFARCEIFLLRDLITVYTFV*