AlfalfaGEDB | MsG0780039697.01

M. sativa cultivar ZhongmuNo.1 / MsG0780039697.01

KEGG: -
GO: GO:0005089
PFAM: PF03759

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780039697.01.T01	XP_039683377.1	97.887	142	3	0	1	142	339	480	1.51E-92	286

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780039697.01.T01	A4IJ27	75.887	141	34	0	1	141	333	473	3.23E-72	226

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780039697.01.T01	Q2HTJ1	97.887	142	3	0	1	142	339	480	7.21e-93	286

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048291.01	MsG0780039697.01	0.821897	3.161471e-53	2.605699e-50
MsG0180003943.01	MsG0780039697.01	0.807197	5.686029e-50	3.147240e-47
MsG0180004049.01	MsG0780039697.01	0.829446	5.123809e-55	5.245185e-52
MsG0180004099.01	MsG0780039697.01	0.859372	4.329360e-63	1.140959e-59
MsG0180004970.01	MsG0780039697.01	0.804540	2.057211e-49	1.063459e-46
MsG0280011014.01	MsG0780039697.01	0.806038	9.987778e-50	5.366099e-47
MsG0680030949.01	MsG0780039697.01	0.826678	2.377850e-54	2.245293e-51
MsG0480019276.01	MsG0780039697.01	0.811663	6.254971e-51	3.894680e-48
MsG0580024489.01	MsG0780039697.01	0.806007	1.013651e-49	5.441962e-47
MsG0380015805.01	MsG0780039697.01	0.806117	9.610755e-50	5.174050e-47
MsG0780036815.01	MsG0780039697.01	0.809619	1.730051e-50	1.020750e-47
MsG0780038424.01	MsG0780039697.01	0.809451	1.880469e-50	1.104429e-47
MsG0780039697.01	MsG0880044952.01	0.800935	1.141111e-48	5.380930e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780039697.01.T01	MTR_7g077690	97.887	142	3	0	1	142	391	532	2.02e-95	285
MsG0780039697.01.T01	MTR_8g030850	82.734	139	24	0	1	139	332	470	1.51e-78	240
MsG0780039697.01.T01	MTR_7g065220	60.000	140	45	2	3	134	412	548	1.65e-47	162
MsG0780039697.01.T01	MTR_5g081410	57.857	140	48	2	3	134	309	445	1.95e-45	155
MsG0780039697.01.T01	MTR_3g069590	56.738	141	50	2	3	135	327	464	2.83e-43	150
MsG0780039697.01.T01	MTR_4g075640	54.610	141	55	3	3	137	314	451	8.47e-40	140
MsG0780039697.01.T01	MTR_4g019770	55.714	140	51	2	3	134	319	455	2.04e-37	134
MsG0780039697.01.T01	MTR_4g013165	46.617	133	64	1	7	139	174	299	1.13e-35	127
MsG0780039697.01.T01	MTR_6g087700	44.526	137	68	2	3	139	318	446	7.61e-34	124
MsG0780039697.01.T01	MTR_2g101830	43.796	137	70	1	3	139	308	437	1.02e-33	123
MsG0780039697.01.T01	MTR_3g069590	53.704	108	39	2	3	102	327	431	5.90e-26	101
MsG0780039697.01.T01	MTR_5g025960	40.288	139	75	2	1	134	336	471	2.09e-24	97.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780039697.01.T01	AT4G00460	75.887	141	34	0	1	141	200	340	2.58e-74	225
MsG0780039697.01.T01	AT4G00460	75.887	141	34	0	1	141	333	473	3.30e-73	226
MsG0780039697.01.T01	AT4G00460	75.887	141	34	0	1	141	333	473	3.30e-73	226
MsG0780039697.01.T01	AT1G01700	76.596	141	33	0	1	141	345	485	1.94e-71	222
MsG0780039697.01.T01	AT1G01700	76.596	141	33	0	1	141	375	515	3.12e-71	222
MsG0780039697.01.T01	AT2G45890	68.085	141	43	2	3	141	322	462	2.11e-62	198
MsG0780039697.01.T01	AT5G02010	62.406	133	44	2	5	134	301	430	3.22e-49	165
MsG0780039697.01.T01	AT1G01700	68.807	109	34	0	1	109	352	460	1.05e-45	154
MsG0780039697.01.T01	AT4G38430	54.930	142	54	3	3	137	317	455	1.59e-43	150
MsG0780039697.01.T01	AT5G05940	56.338	142	49	2	3	134	329	467	9.14e-43	149
MsG0780039697.01.T01	AT3G55660	52.518	139	58	1	3	133	330	468	3.35e-38	136
MsG0780039697.01.T01	AT3G24620	49.219	128	58	1	7	134	310	430	1.19e-35	129
MsG0780039697.01.T01	AT4G13240	48.148	135	62	2	7	141	299	425	9.76e-35	126
MsG0780039697.01.T01	AT1G79860	45.714	140	67	2	3	140	310	442	2.30e-34	125
MsG0780039697.01.T01	AT1G52240	45.926	135	66	1	1	135	320	447	2.21e-32	120
MsG0780039697.01.T01	AT5G19560	45.185	135	65	2	3	135	135	262	1.40e-30	113
MsG0780039697.01.T01	AT5G19560	45.185	135	65	2	3	135	265	392	3.26e-30	113
MsG0780039697.01.T01	AT5G19560	45.185	135	65	2	3	135	265	392	3.26e-30	113
MsG0780039697.01.T01	AT5G19560	45.185	135	65	2	3	135	284	411	4.48e-30	113
MsG0780039697.01.T01	AT3G16130	40.136	147	73	2	1	142	309	445	3.32e-27	105
MsG0780039697.01.T01	AT5G05940	52.727	110	39	2	3	102	329	435	2.67e-26	102
MsG0780039697.01.T01	AT3G55660	49.074	108	47	1	3	102	330	437	9.98e-24	95.5
MsG0780039697.01.T01	AT1G31650	35.172	145	84	3	1	139	326	466	5.56e-22	90.9
MsG0780039697.01.T01	AT1G31650	35.172	145	84	3	1	139	354	494	7.34e-22	90.9
MsG0780039697.01.T01	AT5G19560	38.235	102	54	2	3	102	284	378	1.62e-15	72.4

Find 20 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGAACTTTCACAAACTTCTT+TGG	0.225497	7:+67701003	MsG0780039697.01.T01:CDS
AGAAAGCTATTCAAGAGTTT+TGG	0.272823	7:+67701228	MsG0780039697.01.T01:CDS
CTAAACTTACCTTGTTGTAT+TGG	0.316872	7:-67701039	None:intergenic
GAAAGAAGTAAAAGAGCTTA+TGG	0.337222	7:+67700898	MsG0780039697.01.T01:CDS
GAGACAGTCAAGAAGGCGTT+CGG	0.398257	7:-67700771	None:intergenic
TGGCATTCAACATTGTTGCT+TGG	0.438300	7:+67701257	MsG0780039697.01.T01:CDS
AAAGAAGTTTGTGAAAGTTC+AGG	0.448944	7:-67701001	None:intergenic
AGACAGTCAAGAAGGCGTTC+GGG	0.476515	7:-67700770	None:intergenic
AGCTTATGGATGACACAGAT+TGG	0.490524	7:+67700912	MsG0780039697.01.T01:CDS
AAGCAACAATGTTGAATGCC+AGG	0.498483	7:-67701255	None:intergenic
TCTTTATGTAGATAAATCAA+TGG	0.509070	7:+67701291	MsG0780039697.01.T01:CDS
TTAGTCATTCAAAGTCATCA+TGG	0.545171	7:+67700876	MsG0780039697.01.T01:CDS
GTTCAGGATATCTTTGCTTG+AGG	0.557074	7:-67700985	None:intergenic
AAGCACTTGATCTAGCAGAC+AGG	0.561427	7:+67700813	MsG0780039697.01.T01:CDS
TATGGATGACACAGATTGGA+AGG	0.579189	7:+67700916	MsG0780039697.01.T01:CDS
TTAATTTGAGACAGTCAAGA+AGG	0.590857	7:-67700778	None:intergenic
AGCACTTGATCTAGCAGACA+GGG	0.643640	7:+67700814	MsG0780039697.01.T01:CDS
TTGAATCATCGATGTACACA+TGG	0.667957	7:+67700837	MsG0780039697.01.T01:CDS
TTACCAATATATGTATACTG+TGG	0.673146	7:+67700736	None:intergenic
ATGCAAGATCCAATACAACA+AGG	0.709340	7:+67701030	MsG0780039697.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TCTTTATGTAGATAAATCAA+TGG	+	Chr7:67701291-67701310	MsG0780039697.01.T01:CDS	20.0%
!	AATTGGAGACATTTCTATTT+AGG	+	Chr7:67701152-67701171	MsG0780039697.01.T01:intron	25.0%
	AAAGAAGTTTGTGAAAGTTC+AGG	-	Chr7:67701004-67701023	None:intergenic	30.0%
	GAAAGAAGTAAAAGAGCTTA+TGG	+	Chr7:67700898-67700917	MsG0780039697.01.T01:CDS	30.0%
	TGAACTTTCACAAACTTCTT+TGG	+	Chr7:67701003-67701022	MsG0780039697.01.T01:CDS	30.0%
	TTAGTCATTCAAAGTCATCA+TGG	+	Chr7:67700876-67700895	MsG0780039697.01.T01:CDS	30.0%
!	AGAAAGCTATTCAAGAGTTT+TGG	+	Chr7:67701228-67701247	MsG0780039697.01.T01:CDS	30.0%
!	CTAAACTTACCTTGTTGTAT+TGG	-	Chr7:67701042-67701061	None:intergenic	30.0%
!	GAATGAAAATCATCTTTTGC+AGG	+	Chr7:67701186-67701205	MsG0780039697.01.T01:intron	30.0%
!	TTAATTTGAGACAGTCAAGA+AGG	-	Chr7:67700781-67700800	None:intergenic	30.0%
	ATGCAAGATCCAATACAACA+AGG	+	Chr7:67701030-67701049	MsG0780039697.01.T01:CDS	35.0%
	GCTAGCATATGGAATGAAAT+TGG	+	Chr7:67701135-67701154	MsG0780039697.01.T01:intron	35.0%
	TTGAATCATCGATGTACACA+TGG	+	Chr7:67700837-67700856	MsG0780039697.01.T01:CDS	35.0%
!	AAATCATCTTTTGCAGGATG+TGG	+	Chr7:67701192-67701211	MsG0780039697.01.T01:intron	35.0%
!	AATCATCTTTTGCAGGATGT+GGG	+	Chr7:67701193-67701212	MsG0780039697.01.T01:intron	35.0%
!	TTCATTCCATATGCTAGCTT+AGG	-	Chr7:67701133-67701152	None:intergenic	35.0%
!!!	AGCTATTCAAGAGTTTTGGA+AGG	+	Chr7:67701232-67701251	MsG0780039697.01.T01:CDS	35.0%
	AAGCAACAATGTTGAATGCC+AGG	-	Chr7:67701258-67701277	None:intergenic	40.0%
	AGCTTATGGATGACACAGAT+TGG	+	Chr7:67700912-67700931	MsG0780039697.01.T01:CDS	40.0%
	GTTCAGGATATCTTTGCTTG+AGG	-	Chr7:67700988-67701007	None:intergenic	40.0%
	TATGGATGACACAGATTGGA+AGG	+	Chr7:67700916-67700935	MsG0780039697.01.T01:CDS	40.0%
	TGGCATTCAACATTGTTGCT+TGG	+	Chr7:67701257-67701276	MsG0780039697.01.T01:CDS	40.0%
!	ATCATCTTTTGCAGGATGTG+GGG	+	Chr7:67701194-67701213	MsG0780039697.01.T01:intron	40.0%
	AAGCACTTGATCTAGCAGAC+AGG	+	Chr7:67700813-67700832	MsG0780039697.01.T01:CDS	45.0%
	AGCACTTGATCTAGCAGACA+GGG	+	Chr7:67700814-67700833	MsG0780039697.01.T01:CDS	45.0%
	TCCAGTCCTAAGCTAGCATA+TGG	+	Chr7:67701124-67701143	MsG0780039697.01.T01:intron	45.0%
!	TCCATATGCTAGCTTAGGAC+TGG	-	Chr7:67701128-67701147	None:intergenic	45.0%
!!!	TTCAAGAGTTTTGGAAGGCC+TGG	+	Chr7:67701237-67701256	MsG0780039697.01.T01:CDS	45.0%
!!	AGACAGTCAAGAAGGCGTTC+GGG	-	Chr7:67700773-67700792	None:intergenic	50.0%
!!	GAGACAGTCAAGAAGGCGTT+CGG	-	Chr7:67700774-67700793	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr7	gene	67700746	67701330	67700746	ID=MsG0780039697.01;Name=MsG0780039697.01
Chr7	mRNA	67700746	67701330	67700746	ID=MsG0780039697.01.T01;Parent=MsG0780039697.01;Name=MsG0780039697.01.T01;_AED=0.42;_eAED=0.43;_QI=0\|0\|0\|1\|1\|1\|2\|0\|142
Chr7	exon	67700746	67701051	67700746	ID=MsG0780039697.01.T01:exon:11500;Parent=MsG0780039697.01.T01
Chr7	exon	67701208	67701330	67701208	ID=MsG0780039697.01.T01:exon:11501;Parent=MsG0780039697.01.T01
Chr7	CDS	67700746	67701051	67700746	ID=MsG0780039697.01.T01:cds;Parent=MsG0780039697.01.T01
Chr7	CDS	67701208	67701330	67701208	ID=MsG0780039697.01.T01:cds;Parent=MsG0780039697.01.T01

Gene Sequence

>MsG0780039697.01.T01

ATGTATACTGTGGACAAGTTCTCACCCGAACGCCTTCTTGACTGTCTCAAATTAAGCTCTGAACATGAAGCACTTGATCTAGCAGACAGGGTTGAATCATCGATGTACACATGGAGACGTAAAGCTTGTCTTAGTCATTCAAAGTCATCATGGAAAGAAGTAAAAGAGCTTATGGATGACACAGATTGGAAGGACAAAAACTACATTTTAGCTGATCGAGCAGAAGCTTTGCTGTTTAGCCTCAAGCAAAGATATCCTGAACTTTCACAAACTTCTTTGGACACATGCAAGATCCAATACAACAAGGATGTGGGGAAAGCAGTACTAGAAAGCTATTCAAGAGTTTTGGAAGGCCTGGCATTCAACATTGTTGCTTGGATTGAAGATGTTCTTTATGTAGATAAATCAATGGCGAATCGAGAAGTCTAG

Protein sequence

>MsG0780039697.01.T01

MYTVDKFSPERLLDCLKLSSEHEALDLADRVESSMYTWRRKACLSHSKSSWKEVKELMDDTDWKDKNYILADRAEALLFSLKQRYPELSQTSLDTCKIQYNKDVGKAVLESYSRVLEGLAFNIVAWIEDVLYVDKSMANREV*