AlfalfaGEDB | MsG0180004049.01

M. sativa cultivar ZhongmuNo.1 / MsG0180004049.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004049.01.T01	XP_013468646.1	93.645	299	18	1	1	299	1	298	1.51E-166	474

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004049.01.T01	A0A072VML7	93.645	299	18	1	1	299	1	298	7.20e-167	474

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047974.01	MsG0180004049.01	0.804861	1.762548e-49	9.188216e-47
MsG0080048291.01	MsG0180004049.01	0.867146	1.696740e-65	5.861679e-62
MsG0080048298.01	MsG0180004049.01	0.810951	8.928913e-51	5.457010e-48
MsG0080049140.01	MsG0180004049.01	0.850084	2.133408e-60	4.131025e-57
MsG0180000314.01	MsG0180004049.01	0.825066	5.741136e-54	5.177866e-51
MsG0180000557.01	MsG0180004049.01	0.815610	8.464444e-52	5.863929e-49
MsG0180003786.01	MsG0180004049.01	0.815052	1.126371e-51	7.683889e-49
MsG0180003920.01	MsG0180004049.01	0.855954	4.458254e-62	1.047195e-58
MsG0180003943.01	MsG0180004049.01	0.825274	5.126064e-54	4.650594e-51
MsG0180004049.01	MsG0180004099.01	0.860953	1.441966e-63	4.011918e-60
MsG0180004049.01	MsG0180004389.01	0.815912	7.250835e-52	5.064137e-49
MsG0180004049.01	MsG0180004463.01	0.800593	1.340048e-48	6.264146e-46
MsG0180004049.01	MsG0180004707.01	0.803278	3.762108e-49	1.882768e-46
MsG0180004049.01	MsG0180004711.01	0.833395	5.466158e-56	6.286926e-53
MsG0180004049.01	MsG0180004754.01	0.813487	2.496647e-51	1.632682e-48
MsG0180004049.01	MsG0180004975.01	0.801326	9.490235e-49	4.519444e-46
MsG0180004049.01	MsG0180005575.01	0.804683	1.919976e-49	9.962438e-47
MsG0180004049.01	MsG0180006000.01	0.859715	3.413708e-63	9.101840e-60
MsG0180004049.01	MsG0280006311.01	0.822798	1.953480e-53	1.651870e-50
MsG0180004049.01	MsG0280006405.01	0.804165	2.462884e-49	1.260974e-46
MsG0180004049.01	MsG0280006540.01	0.825641	4.195143e-54	3.846030e-51
MsG0180004049.01	MsG0280006591.01	0.821573	3.758738e-53	3.069473e-50
MsG0180004049.01	MsG0280007359.01	0.811127	8.179284e-51	5.021581e-48
MsG0180004049.01	MsG0280007452.01	0.838726	2.422435e-57	3.273374e-54
MsG0180004049.01	MsG0280007506.01	0.820741	5.845003e-53	4.663673e-50
MsG0180004049.01	MsG0280007529.01	0.822430	2.378375e-53	1.990384e-50
MsG0180004049.01	MsG0280008280.01	0.808289	3.332327e-50	1.897956e-47
MsG0180004049.01	MsG0280009514.01	0.811378	7.212499e-51	4.457525e-48
MsG0180004049.01	MsG0280009752.01	0.826654	2.409669e-54	2.273740e-51
MsG0180004049.01	MsG0280009783.01	0.820628	6.203899e-53	4.934269e-50
MsG0180004049.01	MsG0280009794.01	0.866811	2.169235e-65	7.409353e-62
MsG0180004049.01	MsG0280010015.01	0.843028	1.801795e-58	2.782571e-55
MsG0180004049.01	MsG0280010650.01	0.807574	4.729717e-50	2.643578e-47
MsG0180004049.01	MsG0280010778.01	0.820275	7.474452e-53	5.887564e-50
MsG0180004049.01	MsG0280010779.01	0.850365	1.780176e-60	3.478661e-57
MsG0180004049.01	MsG0280010977.01	0.818536	1.862442e-52	1.397920e-49
MsG0180004049.01	MsG0280011003.01	0.835518	1.601175e-56	1.962675e-53
MsG0180004049.01	MsG0280011013.01	0.824901	6.277781e-54	5.635722e-51
MsG0180004049.01	MsG0280011014.01	0.847676	9.946216e-60	1.781526e-56
MsG0180004049.01	MsG0280011101.01	0.804410	2.189524e-49	1.128073e-46
MsG0180004049.01	MsG0280011154.01	0.812868	3.414047e-51	2.195262e-48
MsG0180004049.01	MsG0280011258.01	0.800635	1.313764e-48	6.147922e-46
MsG0180004049.01	MsG0280011282.01	0.833580	4.915205e-56	5.684414e-53
MsG0180004049.01	MsG0280011341.01	0.820127	8.083454e-53	6.341801e-50
MsG0180004049.01	MsG0280011416.01	0.839354	1.666599e-57	2.295716e-54
MsG0180004049.01	MsG0380012471.01	0.826130	3.211440e-54	2.985461e-51
MsG0180004049.01	MsG0380014289.01	0.811650	6.296257e-51	3.919041e-48
MsG0180004049.01	MsG0380014387.01	0.822870	1.879559e-53	1.592651e-50
MsG0180004049.01	MsG0380014537.01	0.814070	1.858168e-51	1.234445e-48
MsG0180004049.01	MsG0380014703.01	0.805717	1.166791e-49	6.217690e-47
MsG0180004049.01	MsG0380014808.01	0.815334	9.751446e-52	6.704024e-49
MsG0180004049.01	MsG0380015332.01	0.820953	5.223363e-53	4.192942e-50
MsG0180004049.01	MsG0380015548.01	0.840137	1.042160e-57	1.470807e-54
MsG0180004049.01	MsG0380015631.01	0.824476	7.908940e-54	7.012628e-51
MsG0180004049.01	MsG0380015677.01	0.814459	1.524164e-51	1.023297e-48
MsG0180004049.01	MsG0380016140.01	0.833717	4.541515e-56	5.273729e-53
MsG0180004049.01	MsG0380016517.01	0.811241	7.724652e-51	4.756769e-48
MsG0180004049.01	MsG0380016703.01	0.834260	3.321329e-56	3.920671e-53
MsG0180004049.01	MsG0380016893.01	0.804412	2.187298e-49	1.126988e-46
MsG0180004049.01	MsG0380016919.01	0.814348	1.613223e-51	1.079816e-48
MsG0180004049.01	MsG0380016920.01	0.854499	1.182235e-61	2.645500e-58
MsG0180004049.01	MsG0380016970.01	0.811099	8.293534e-51	5.088043e-48
MsG0180004049.01	MsG0380016994.01	0.840862	6.733658e-58	9.718936e-55
MsG0180004049.01	MsG0380017049.01	0.803663	3.130861e-49	1.582398e-46
MsG0180004049.01	MsG0380017159.01	0.834997	2.168101e-56	2.616431e-53
MsG0180004049.01	MsG0380017996.01	0.830669	2.577875e-55	2.734579e-52
MsG0180004049.01	MsG0480018397.01	0.859519	3.911473e-63	1.035814e-59
MsG0180004049.01	MsG0480018919.01	0.825014	5.905418e-54	5.318514e-51
MsG0180004049.01	MsG0480019276.01	0.805870	1.083187e-49	5.794848e-47
MsG0180004049.01	MsG0480019350.01	0.824647	7.207240e-54	6.423164e-51
MsG0180004049.01	MsG0480019710.01	0.831071	2.054302e-55	2.205155e-52
MsG0180004049.01	MsG0480019922.01	0.862436	5.074538e-64	1.486174e-60
MsG0180004049.01	MsG0480020402.01	0.834922	2.265269e-56	2.727542e-53
MsG0180004049.01	MsG0480020495.01	0.859010	5.557622e-63	1.446332e-59
MsG0180004049.01	MsG0480020699.01	0.818913	1.529669e-52	1.160221e-49
MsG0180004049.01	MsG0480020739.01	0.837072	6.448333e-57	8.282486e-54
MsG0180004049.01	MsG0480020847.01	0.849873	2.444696e-60	4.700864e-57
MsG0180004049.01	MsG0480020909.01	0.821492	3.923473e-53	3.196696e-50
MsG0180004049.01	MsG0480020965.01	0.804752	1.857312e-49	9.654976e-47
MsG0180004049.01	MsG0480021138.01	0.816302	5.932348e-52	4.188144e-49
MsG0180004049.01	MsG0480021491.01	0.808662	2.774401e-50	1.595546e-47
MsG0180004049.01	MsG0480022045.01	0.814314	1.640676e-51	1.097244e-48
MsG0180004049.01	MsG0480022164.01	0.809066	2.273491e-50	1.321650e-47
MsG0180004049.01	MsG0480022325.01	0.820984	5.136915e-53	4.127179e-50
MsG0180004049.01	MsG0480022331.01	0.803270	3.776699e-49	1.889684e-46
MsG0180004049.01	MsG0480022549.01	0.817939	2.542608e-52	1.877224e-49
MsG0180004049.01	MsG0480022620.01	0.803454	3.458977e-49	1.738931e-46
MsG0180004049.01	MsG0480023130.01	0.811249	7.695548e-51	4.739839e-48
MsG0180004049.01	MsG0480023262.01	0.831170	1.942713e-55	2.091484e-52
MsG0180004049.01	MsG0480023341.01	0.818529	1.869427e-52	1.402888e-49
MsG0180004049.01	MsG0480023463.01	0.848886	4.603877e-60	8.573124e-57
MsG0180004049.01	MsG0480023600.01	0.817570	3.079404e-52	2.250649e-49
MsG0180004049.01	MsG0480023672.01	0.808800	2.592107e-50	1.496129e-47
MsG0180004049.01	MsG0480023762.01	0.810814	9.559614e-51	5.820540e-48
MsG0180004049.01	MsG0480023832.01	0.805819	1.110284e-49	5.931986e-47
MsG0180004049.01	MsG0580024358.01	0.806039	9.981265e-50	5.362765e-47
MsG0180004049.01	MsG0580024463.01	0.802057	6.719438e-49	3.259758e-46
MsG0180004049.01	MsG0580024489.01	0.874821	5.004160e-68	2.285423e-64
MsG0180004049.01	MsG0580024758.01	0.833497	5.154310e-56	5.946300e-53
MsG0180004049.01	MsG0580025614.01	0.821411	4.095746e-53	3.329584e-50
MsG0180004049.01	MsG0580025766.01	0.807258	5.519010e-50	3.059677e-47
MsG0180004049.01	MsG0580026059.01	0.835977	1.225506e-56	1.523072e-53
MsG0180004049.01	MsG0580026089.01	0.804442	2.155610e-49	1.111533e-46
MsG0180004049.01	MsG0580026593.01	0.837717	4.408328e-57	5.776293e-54
MsG0180004049.01	MsG0580027031.01	0.805844	1.096834e-49	5.863884e-47
MsG0180004049.01	MsG0580027431.01	0.821473	3.962600e-53	3.226898e-50
MsG0180004049.01	MsG0580027650.01	0.804694	1.910273e-49	9.914853e-47
MsG0180004049.01	MsG0580028756.01	0.820058	8.381359e-53	6.562788e-50
MsG0180004049.01	MsG0580029298.01	0.813946	1.978588e-51	1.309968e-48
MsG0180004049.01	MsG0580029640.01	0.836027	1.190189e-56	1.481332e-53
MsG0180004049.01	MsG0680030949.01	0.867024	1.855703e-65	6.383822e-62
MsG0180004049.01	MsG0680031214.01	0.834918	2.269445e-56	2.732290e-53
MsG0180004049.01	MsG0680031488.01	0.811164	8.026782e-51	4.932761e-48
MsG0180004049.01	MsG0680031506.01	0.840503	8.359193e-58	1.193173e-54
MsG0180004049.01	MsG0680034598.01	0.802144	6.448732e-49	3.135319e-46
MsG0180004049.01	MsG0680034853.01	0.853768	1.921164e-61	4.196676e-58
MsG0180004049.01	MsG0680035475.01	0.802703	4.946093e-49	2.439318e-46
MsG0180004049.01	MsG0780036147.01	0.801172	1.020557e-48	4.841276e-46
MsG0180004049.01	MsG0780036477.01	0.827759	1.310017e-54	1.276496e-51
MsG0180004049.01	MsG0780036752.01	0.853804	1.875390e-61	4.101800e-58
MsG0180004049.01	MsG0780036815.01	0.845504	3.897563e-59	6.508823e-56
MsG0180004049.01	MsG0780036816.01	0.814295	1.656629e-51	1.107322e-48
MsG0180004049.01	MsG0780038332.01	0.801080	1.065939e-48	5.044981e-46
MsG0180004049.01	MsG0780038401.01	0.834156	3.527922e-56	4.150916e-53
MsG0180004049.01	MsG0780038424.01	0.825890	3.662567e-54	3.381795e-51
MsG0180004049.01	MsG0780038603.01	0.815380	9.524509e-52	6.556142e-49
MsG0180004049.01	MsG0780039137.01	0.815491	8.998814e-52	6.213566e-49
MsG0180004049.01	MsG0780039243.01	0.802625	5.133021e-49	2.526401e-46
MsG0180004049.01	MsG0780039697.01	0.829446	5.123809e-55	5.245185e-52
MsG0180004049.01	MsG0780039866.01	0.844570	6.965416e-59	1.129282e-55
MsG0180004049.01	MsG0780040057.01	0.842252	2.897391e-58	4.367329e-55
MsG0180004049.01	MsG0780040307.01	0.855236	7.224945e-62	1.656660e-58
MsG0180004049.01	MsG0780040683.01	0.814470	1.515391e-51	1.017760e-48
MsG0180004049.01	MsG0780040841.01	0.831882	1.297469e-55	1.426819e-52
MsG0180004049.01	MsG0780040896.01	0.808157	3.555514e-50	2.018052e-47
MsG0180004049.01	MsG0780040926.01	0.800217	1.598326e-48	7.400669e-46
MsG0180004049.01	MsG0780041063.01	0.842363	2.706174e-58	4.093869e-55
MsG0180004049.01	MsG0780041132.01	0.812208	4.758854e-51	3.007172e-48
MsG0180004049.01	MsG0780041535.01	0.834461	2.958093e-56	3.513044e-53
MsG0180004049.01	MsG0780041592.01	0.801449	8.957848e-49	4.279111e-46
MsG0180004049.01	MsG0780041735.01	0.804382	2.218559e-49	1.142275e-46
MsG0180004049.01	MsG0880041860.01	0.802776	4.777358e-49	2.360548e-46
MsG0180004049.01	MsG0880042380.01	0.814964	1.178046e-51	8.017537e-49
MsG0180004049.01	MsG0880042773.01	0.808240	3.413480e-50	1.941688e-47
MsG0180004049.01	MsG0880042961.01	0.805474	1.312189e-49	6.948484e-47
MsG0180004049.01	MsG0880043034.01	0.843228	1.594217e-58	2.477671e-55
MsG0180004049.01	MsG0880043516.01	0.839601	1.436892e-57	1.994208e-54
MsG0180004049.01	MsG0880043614.01	0.860409	2.107437e-63	5.754715e-60
MsG0180004049.01	MsG0880043637.01	0.814637	1.392307e-51	9.392708e-49
MsG0180004049.01	MsG0880044782.01	0.833762	4.425600e-56	5.146510e-53
MsG0180004049.01	MsG0880045145.01	0.801468	8.875395e-49	4.241795e-46
MsG0180004049.01	MsG0880045506.01	0.840423	8.776574e-58	1.249572e-54
MsG0180004049.01	MsG0880046012.01	0.803248	3.817321e-49	1.908909e-46
MsG0180004049.01	MsG0880046649.01	0.812838	3.466252e-51	2.227078e-48
MsG0180004049.01	MsG0880046803.01	0.807469	4.978416e-50	2.775259e-47
MsG0180004049.01	MsG0880047226.01	0.834840	2.374824e-56	2.852397e-53
MsG0180004049.01	MsG0880047472.01	0.806149	9.464146e-50	5.099163e-47
MsG0180004049.01	MsG0880047473.01	0.822240	2.632896e-53	2.191334e-50

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004049.01.T01	MTR_1g073670	93.645	299	18	1	1	299	1	298	1.83e-170	474
MsG0180004049.01.T01	MTR_1g069610	26.667	345	182	14	1	298	1	321	3.46e-14	72.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004049.01.T01	AT2G33400	29.967	307	138	10	1	288	1	249	2.26e-25	102
MsG0180004049.01.T01	AT5G44040	32.759	290	141	11	36	298	77	339	2.91e-18	84.7
MsG0180004049.01.T01	AT1G04030	28.086	324	173	14	1	285	1	303	1.05e-12	68.6
MsG0180004049.01.T01	AT1G04030	28.086	324	173	14	1	285	1	303	1.05e-12	68.6

Find 66 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GATGAAAGTTTGGAGAATTT+TGG	0.099083	1:-72484924	MsG0180004049.01.T01:CDS
AAAGAAAGAAAGAAAGAAAA+TGG	0.158174	1:-72485592	MsG0180004049.01.T01:exon
CCCTAGTTCTTATCAGGTTT+TGG	0.222472	1:-72485153	MsG0180004049.01.T01:CDS
ACAAACCTGATTTGAGAATA+TGG	0.294465	1:+72485356	None:intergenic
GGCAAACCCTAGTTCTTATC+AGG	0.299458	1:-72485159	MsG0180004049.01.T01:CDS
GTTATTTCAGCTTGTGCTAC+TGG	0.317321	1:+72484135	None:intergenic
TTTCTTTCTTTCTCTTTCAA+TGG	0.327083	1:+72485604	None:intergenic
TTGTACCATATTCTCAAATC+AGG	0.328302	1:-72485361	MsG0180004049.01.T01:intron
TGATGAGGAAGATGAAAATA+AGG	0.334449	1:-72485123	MsG0180004049.01.T01:CDS
TGATTGGGATGATGAAAGTT+TGG	0.346270	1:-72484934	MsG0180004049.01.T01:CDS
TTCATTCATTAAAAGTTTGT+TGG	0.384333	1:-72484419	MsG0180004049.01.T01:three_prime_UTR
TCATTCATTAAAAGTTTGTT+GGG	0.396716	1:-72484418	MsG0180004049.01.T01:three_prime_UTR
GGAGGATGAAATAGTGTATG+AGG	0.408167	1:-72485003	MsG0180004049.01.T01:CDS
AAGAAAGAAAGAAAGAAAAT+GGG	0.409644	1:-72485591	MsG0180004049.01.T01:exon
CAGCTTGTGCTACTGGTCCC+TGG	0.429961	1:+72484142	None:intergenic
GAAGTTCTCGCCTTGCGTCT+TGG	0.432670	1:-72484640	MsG0180004049.01.T01:CDS
TCAGTCGAAGCCTTTGTTGC+AGG	0.437491	1:-72484604	MsG0180004049.01.T01:CDS
AATTTGATGAAGGGTATGAT+TGG	0.441432	1:-72484950	MsG0180004049.01.T01:CDS
TGCTAGTCTTTCAAACTGGT+TGG	0.449139	1:-72484568	MsG0180004049.01.T01:CDS
AAGCATGCTGCCTGTAGTCT+TGG	0.451334	1:-72484673	MsG0180004049.01.T01:CDS
TTTGAATGTAGAGATGTTTG+AGG	0.463362	1:-72485180	MsG0180004049.01.T01:CDS
CAACAAAGGCTTCGACTGAA+TGG	0.464145	1:+72484608	None:intergenic
TTGATGCTAGTCTTTCAAAC+TGG	0.480004	1:-72484572	MsG0180004049.01.T01:CDS
AACAGTGAACAGAAAGAAGA+TGG	0.482964	1:-72484879	MsG0180004049.01.T01:CDS
ATGTTGCAGTATGCAATTTG+AGG	0.485238	1:-72484282	MsG0180004049.01.T01:three_prime_UTR
GATGATGATGAATTTGATGA+AGG	0.486925	1:-72484960	MsG0180004049.01.T01:CDS
TACAACTGTATACAAACTCC+AGG	0.487392	1:-72484160	MsG0180004049.01.T01:three_prime_UTR
TAGAGGTAGAGATCAAGAAA+AGG	0.488908	1:-72485249	MsG0180004049.01.T01:CDS
CATTGAGTAAGATTCTCTAC+CGG	0.490839	1:+72484768	None:intergenic
CGGTAGAGAATCTTACTCAA+TGG	0.500520	1:-72484767	MsG0180004049.01.T01:CDS
ACTGCAACTTCCTGCAACAA+AGG	0.507022	1:+72484594	None:intergenic
TGTCTAAAACAGAAACTCGT+TGG	0.507304	1:-72485763	MsG0180004049.01.T01:five_prime_UTR
ACTCTCTACAAAGTCCTAGC+TGG	0.507971	1:-72485519	MsG0180004049.01.T01:CDS
GAGCTTGATTCCAAGACTAC+AGG	0.512568	1:+72484663	None:intergenic
ATAAGAAAGAATGTTTATGT+TGG	0.519157	1:+72485639	None:intergenic
CATGAATTCCGTTCTGCACC+CGG	0.525680	1:-72484787	MsG0180004049.01.T01:CDS
TCCAAAACCTGATAAGAACT+AGG	0.528369	1:+72485152	None:intergenic
ATCAAGCAAACAAACGAGAA+AGG	0.534474	1:-72484721	MsG0180004049.01.T01:CDS
ATTCGACGGGTATCGAATCA+TGG	0.538164	1:+72485061	None:intergenic
TTATGATATCTATGATTCGA+CGG	0.557585	1:+72485047	None:intergenic
CCAAAACCTGATAAGAACTA+GGG	0.562445	1:+72485153	None:intergenic
AGAAACTCGTTGGAGCCAAA+CGG	0.563148	1:-72485753	MsG0180004049.01.T01:five_prime_UTR
ATGTTTGAATCAGTTAAAAG+TGG	0.563975	1:-72484231	MsG0180004049.01.T01:three_prime_UTR
GATCAAGAAAAGGAGAAGAG+TGG	0.564885	1:-72485239	MsG0180004049.01.T01:CDS
TATGATATCTATGATTCGAC+GGG	0.566426	1:+72485048	None:intergenic
TTTCTGTTTCTTCAAGCACT+CGG	0.567496	1:+72484496	None:intergenic
TAGTTATGACAATGAGGAGG+AGG	0.567704	1:-72485021	MsG0180004049.01.T01:CDS
ATTTGATGAAGGGTATGATT+GGG	0.573696	1:-72484949	MsG0180004049.01.T01:CDS
ATGATGATGAATTTGATGAA+GGG	0.582122	1:-72484959	MsG0180004049.01.T01:CDS
ATTGAGTAAGATTCTCTACC+GGG	0.594847	1:+72484769	None:intergenic
TCTCAAATCTTATGCTCACA+AGG	0.600946	1:-72484256	MsG0180004049.01.T01:three_prime_UTR
ATAGGTTGTCAATATATGAA+AGG	0.605599	1:-72484183	MsG0180004049.01.T01:three_prime_UTR
CGAATTTGAGTGGACGAGAG+AGG	0.606574	1:-72484818	MsG0180004049.01.T01:CDS
GTAGATAATCTAAGCAACAT+AGG	0.610188	1:-72484201	MsG0180004049.01.T01:three_prime_UTR
ATTCTCTACCGGGTGCAGAA+CGG	0.617190	1:+72484779	None:intergenic
GAATTGAAATCGAATTTGAG+TGG	0.620274	1:-72484828	MsG0180004049.01.T01:CDS
TCATTGACAACGAATTGTAG+TGG	0.620784	1:+72484524	None:intergenic
ACAATTCGTTGTCAATGATG+TGG	0.639822	1:-72484519	MsG0180004049.01.T01:exon
ATCGAATTGTGATGCTTCTG+TGG	0.643973	1:-72484454	MsG0180004049.01.T01:three_prime_UTR
TGAAAATAAGGAGACTACTG+TGG	0.644236	1:-72485111	MsG0180004049.01.T01:CDS
TAACTGTAGTTATGACAATG+AGG	0.650246	1:-72485027	MsG0180004049.01.T01:CDS
GCATTCGATTCCAAGACGCA+AGG	0.653152	1:+72484630	None:intergenic
GTAGTGGAGACATTATAACT+AGG	0.665616	1:+72484540	None:intergenic
ACTGTGGAATCAGAGAGAGA+AGG	0.685984	1:-72485095	MsG0180004049.01.T01:CDS
CTGTAGTTATGACAATGAGG+AGG	0.688122	1:-72485024	MsG0180004049.01.T01:CDS
ACAACTGTATACAAACTCCA+GGG	0.699450	1:-72484159	MsG0180004049.01.T01:three_prime_UTR

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTTTAATTTATTTGTTAG+AGG	-	Chr1:72484584-72484603	MsG0180004049.01.T01:CDS	10.0%
!!	AAAGAAAGAAAGAAAGAAAA+TGG	-	Chr1:72484258-72484277	MsG0180004049.01.T01:three_prime_UTR	20.0%
!!	AAGAAAGAAAGAAAGAAAAT+GGG	-	Chr1:72484259-72484278	MsG0180004049.01.T01:three_prime_UTR	20.0%
!!	ATAAGAAAGAATGTTTATGT+TGG	+	Chr1:72484214-72484233	None:intergenic	20.0%
!!	TGAAGAAATATTATTGTATG+TGG	-	Chr1:72484536-72484555	MsG0180004049.01.T01:CDS	20.0%
!!	TTCATTCATTAAAAGTTTGT+TGG	-	Chr1:72485431-72485450	MsG0180004049.01.T01:intron	20.0%
!!!	TCATTCATTAAAAGTTTGTT+GGG	-	Chr1:72485432-72485451	MsG0180004049.01.T01:intron	20.0%
!	AATCAAGAACTTAAGAATGA+TGG	+	Chr1:72484386-72484405	None:intergenic	25.0%
!	ACCAAAAACAAAACAATTAC+AGG	+	Chr1:72485492-72485511	None:intergenic	25.0%
!	ATAGGTTGTCAATATATGAA+AGG	-	Chr1:72485667-72485686	MsG0180004049.01.T01:five_prime_UTR	25.0%
!	ATCAAGAACTTAAGAATGAT+GGG	+	Chr1:72484385-72484404	None:intergenic	25.0%
!	ATGTTTGAATCAGTTAAAAG+TGG	-	Chr1:72485619-72485638	MsG0180004049.01.T01:five_prime_UTR	25.0%
!	TTATGATATCTATGATTCGA+CGG	+	Chr1:72484806-72484825	None:intergenic	25.0%
!	TTTCTTTCTTTCTCTTTCAA+TGG	+	Chr1:72484249-72484268	None:intergenic	25.0%
!!	ATGAAAGTTTGGAGAATTTT+GGG	-	Chr1:72484927-72484946	MsG0180004049.01.T01:CDS	25.0%
!!	ATGATGATGAATTTGATGAA+GGG	-	Chr1:72484891-72484910	MsG0180004049.01.T01:CDS	25.0%
!!	CTTATCTTTTTGCAGAAATA+TGG	-	Chr1:72484428-72484447	MsG0180004049.01.T01:three_prime_UTR	25.0%
!!!	GTTGTTTTGATATACTTACT+TGG	+	Chr1:72484363-72484382	None:intergenic	25.0%
!!!	TCCTGTAATTGTTTTGTTTT+TGG	-	Chr1:72485488-72485507	MsG0180004049.01.T01:intron	25.0%
!!!	TTTTGTTTTTGGTTTGTGAA+TGG	-	Chr1:72485499-72485518	MsG0180004049.01.T01:intron	25.0%
	GAATTGAAATCGAATTTGAG+TGG	-	Chr1:72485022-72485041	MsG0180004049.01.T01:CDS	30.0%
	GTAGATAATCTAAGCAACAT+AGG	-	Chr1:72485649-72485668	MsG0180004049.01.T01:five_prime_UTR	30.0%
	TAACTGTAGTTATGACAATG+AGG	-	Chr1:72484823-72484842	MsG0180004049.01.T01:CDS	30.0%
	TATGATATCTATGATTCGAC+GGG	+	Chr1:72484805-72484824	None:intergenic	30.0%
	TGATGAGGAAGATGAAAATA+AGG	-	Chr1:72484727-72484746	MsG0180004049.01.T01:CDS	30.0%
	TTGTACCATATTCTCAAATC+AGG	-	Chr1:72484489-72484508	MsG0180004049.01.T01:three_prime_UTR	30.0%
	TTTGAATGTAGAGATGTTTG+AGG	-	Chr1:72484670-72484689	MsG0180004049.01.T01:CDS	30.0%
!	AATTTGATGAAGGGTATGAT+TGG	-	Chr1:72484900-72484919	MsG0180004049.01.T01:CDS	30.0%
!	ACAAACCTGATTTGAGAATA+TGG	+	Chr1:72484497-72484516	None:intergenic	30.0%
!	ATTTGATGAAGGGTATGATT+GGG	-	Chr1:72484901-72484920	MsG0180004049.01.T01:CDS	30.0%
!	GATGAAAGTTTGGAGAATTT+TGG	-	Chr1:72484926-72484945	MsG0180004049.01.T01:CDS	30.0%
!	GATGATGATGAATTTGATGA+AGG	-	Chr1:72484890-72484909	MsG0180004049.01.T01:CDS	30.0%
!	TGAAAGTTTGGAGAATTTTG+GGG	-	Chr1:72484928-72484947	MsG0180004049.01.T01:CDS	30.0%
	AACAGTGAACAGAAAGAAGA+TGG	-	Chr1:72484971-72484990	MsG0180004049.01.T01:CDS	35.0%
	ACAACTGTATACAAACTCCA+GGG	-	Chr1:72485691-72485710	MsG0180004049.01.T01:five_prime_UTR	35.0%
	ACAATTCGTTGTCAATGATG+TGG	-	Chr1:72485331-72485350	MsG0180004049.01.T01:intron	35.0%
	ATCAAGCAAACAAACGAGAA+AGG	-	Chr1:72485129-72485148	MsG0180004049.01.T01:CDS	35.0%
	ATGTTGCAGTATGCAATTTG+AGG	-	Chr1:72485568-72485587	MsG0180004049.01.T01:CDS	35.0%
	ATTGAGTAAGATTCTCTACC+GGG	+	Chr1:72485084-72485103	None:intergenic	35.0%
	ATTGTATGTGGATCTGTGAA+TGG	-	Chr1:72484548-72484567	MsG0180004049.01.T01:CDS	35.0%
	CATTGAGTAAGATTCTCTAC+CGG	+	Chr1:72485085-72485104	None:intergenic	35.0%
	CCAAAACCTGATAAGAACTA+GGG	+	Chr1:72484700-72484719	None:intergenic	35.0%
	TACAACTGTATACAAACTCC+AGG	-	Chr1:72485690-72485709	MsG0180004049.01.T01:five_prime_UTR	35.0%
	TAGAGGTAGAGATCAAGAAA+AGG	-	Chr1:72484601-72484620	MsG0180004049.01.T01:CDS	35.0%
	TCATTGACAACGAATTGTAG+TGG	+	Chr1:72485329-72485348	None:intergenic	35.0%
	TCCAAAACCTGATAAGAACT+AGG	+	Chr1:72484701-72484720	None:intergenic	35.0%
	TCTCAAATCTTATGCTCACA+AGG	-	Chr1:72485594-72485613	MsG0180004049.01.T01:exon	35.0%
	TGAAAATAAGGAGACTACTG+TGG	-	Chr1:72484739-72484758	MsG0180004049.01.T01:CDS	35.0%
	TGATTGGGATGATGAAAGTT+TGG	-	Chr1:72484916-72484935	MsG0180004049.01.T01:CDS	35.0%
!	ATTTTGCTACAAGGGTTCTT+TGG	+	Chr1:72485740-72485759	None:intergenic	35.0%
!	GTAGTGGAGACATTATAACT+AGG	+	Chr1:72485313-72485332	None:intergenic	35.0%
!	TGTCTAAAACAGAAACTCGT+TGG	-	Chr1:72484087-72484106	MsG0180004049.01.T01:three_prime_UTR	35.0%
!	TTTCTGTTTCTTCAAGCACT+CGG	+	Chr1:72485357-72485376	None:intergenic	35.0%
!!	GGTTGTTTTCTTCAGTGTTT+TGG	-	Chr1:72484280-72484299	MsG0180004049.01.T01:three_prime_UTR	35.0%
!!	TTGATGCTAGTCTTTCAAAC+TGG	-	Chr1:72485278-72485297	MsG0180004049.01.T01:intron	35.0%
!!!	ATGCTTGTTTTTTGCTCTGA+TGG	+	Chr1:72485163-72485182	None:intergenic	35.0%
!!!	GACAAAGTTTTAAAGCCGTT+TGG	+	Chr1:72484115-72484134	None:intergenic	35.0%
	ATCGAATTGTGATGCTTCTG+TGG	-	Chr1:72485396-72485415	MsG0180004049.01.T01:CDS	40.0%
	CGGTAGAGAATCTTACTCAA+TGG	-	Chr1:72485083-72485102	MsG0180004049.01.T01:CDS	40.0%
	CTGTAGTTATGACAATGAGG+AGG	-	Chr1:72484826-72484845	MsG0180004049.01.T01:CDS	40.0%
	GATCAAGAAAAGGAGAAGAG+TGG	-	Chr1:72484611-72484630	MsG0180004049.01.T01:CDS	40.0%
	GGAGGATGAAATAGTGTATG+AGG	-	Chr1:72484847-72484866	MsG0180004049.01.T01:CDS	40.0%
	TAGTTATGACAATGAGGAGG+AGG	-	Chr1:72484829-72484848	MsG0180004049.01.T01:CDS	40.0%
!	ACTCGTGCAATTTTGCTACA+AGG	+	Chr1:72485749-72485768	None:intergenic	40.0%
!	CTCGTGCAATTTTGCTACAA+GGG	+	Chr1:72485748-72485767	None:intergenic	40.0%
!	GTTATTTCAGCTTGTGCTAC+TGG	+	Chr1:72485718-72485737	None:intergenic	40.0%
!!	CCCTAGTTCTTATCAGGTTT+TGG	-	Chr1:72484697-72484716	MsG0180004049.01.T01:CDS	40.0%
!!	TGCTAGTCTTTCAAACTGGT+TGG	-	Chr1:72485282-72485301	MsG0180004049.01.T01:intron	40.0%
!!	TTACTTGGTTTTCTCCAGCT+AGG	+	Chr1:72484348-72484367	None:intergenic	40.0%
!!!	GGTTTTGGATGATGATGATG+AGG	-	Chr1:72484712-72484731	MsG0180004049.01.T01:CDS	40.0%
	ACTCTCTACAAAGTCCTAGC+TGG	-	Chr1:72484331-72484350	MsG0180004049.01.T01:three_prime_UTR	45.0%
	ACTGCAACTTCCTGCAACAA+AGG	+	Chr1:72485259-72485278	None:intergenic	45.0%
	ACTGTGGAATCAGAGAGAGA+AGG	-	Chr1:72484755-72484774	MsG0180004049.01.T01:CDS	45.0%
	AGAAACTCGTTGGAGCCAAA+CGG	-	Chr1:72484097-72484116	MsG0180004049.01.T01:three_prime_UTR	45.0%
	CAACAAAGGCTTCGACTGAA+TGG	+	Chr1:72485245-72485264	None:intergenic	45.0%
	GAGCTTGATTCCAAGACTAC+AGG	+	Chr1:72485190-72485209	None:intergenic	45.0%
!	GGCAAACCCTAGTTCTTATC+AGG	-	Chr1:72484691-72484710	MsG0180004049.01.T01:CDS	45.0%
!!	ATTCGACGGGTATCGAATCA+TGG	+	Chr1:72484792-72484811	None:intergenic	45.0%
	AAGCATGCTGCCTGTAGTCT+TGG	-	Chr1:72485177-72485196	MsG0180004049.01.T01:CDS	50.0%
	ATTCTCTACCGGGTGCAGAA+CGG	+	Chr1:72485074-72485093	None:intergenic	50.0%
	CATGAATTCCGTTCTGCACC+CGG	-	Chr1:72485063-72485082	MsG0180004049.01.T01:CDS	50.0%
	CGAATTTGAGTGGACGAGAG+AGG	-	Chr1:72485032-72485051	MsG0180004049.01.T01:CDS	50.0%
	GCATTCGATTCCAAGACGCA+AGG	+	Chr1:72485223-72485242	None:intergenic	50.0%
	TCAGTCGAAGCCTTTGTTGC+AGG	-	Chr1:72485246-72485265	MsG0180004049.01.T01:CDS	50.0%
	GAAGTTCTCGCCTTGCGTCT+TGG	-	Chr1:72485210-72485229	MsG0180004049.01.T01:CDS	55.0%
!	CAGCTTGTGCTACTGGTCCC+TGG	+	Chr1:72485711-72485730	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	72484075	72485797	72484075	ID=MsG0180004049.01;Name=MsG0180004049.01
Chr1	mRNA	72484075	72485797	72484075	ID=MsG0180004049.01.T01;Parent=MsG0180004049.01;Name=MsG0180004049.01.T01;_AED=0.49;_eAED=0.49;_QI=202\|1\|1\|1\|1\|1\|3\|449\|299
Chr1	exon	72484075	72485268	72484075	ID=MsG0180004049.01.T01:exon:5072;Parent=MsG0180004049.01.T01
Chr1	exon	72485362	72485429	72485362	ID=MsG0180004049.01.T01:exon:5071;Parent=MsG0180004049.01.T01
Chr1	exon	72485509	72485797	72485509	ID=MsG0180004049.01.T01:exon:5070;Parent=MsG0180004049.01.T01
Chr1	five_prime_UTR	72485596	72485797	72485596	ID=MsG0180004049.01.T01:five_prime_utr;Parent=MsG0180004049.01.T01
Chr1	CDS	72485509	72485595	72485509	ID=MsG0180004049.01.T01:cds;Parent=MsG0180004049.01.T01
Chr1	CDS	72485362	72485429	72485362	ID=MsG0180004049.01.T01:cds;Parent=MsG0180004049.01.T01
Chr1	CDS	72484524	72485268	72484524	ID=MsG0180004049.01.T01:cds;Parent=MsG0180004049.01.T01
Chr1	three_prime_UTR	72484075	72484523	72484075	ID=MsG0180004049.01.T01:three_prime_utr;Parent=MsG0180004049.01.T01

Gene Sequence

>MsG0180004049.01.T01

ATGGGTTGTTTTCTTCAGTGTTTTGGTTTAACAAGCAAAAGAAAACGCAGAAAGACTCTCTACAAAGTCCTAGCTGGAGAAAACCAAAAATATGGAAACTATCAAGTTCTTGCTACTATTACAGAGAAATCTATTGTACCATATTCTCAAATCAGAGGTAGAGATCAAGAAAAGGAGAAGAGTGGTGTTAAGAGCAAGAAGAAGAAGAAAGTTAGCTTTAATTTGAATGTAGAGATGTTTGAGGCAAACCCTAGTTCTTATCAGGTTTTGGATGATGATGATGAGGAAGATGAAAATAAGGAGACTACTGTGGAATCAGAGAGAGAAGGATCTGTAGTGTCCATGATTCGATACCCGTCGAATCATAGATATCATAACTGTAGTTATGACAATGAGGAGGAGGATGAAATAGTGTATGAGGAATCTGATATTGATGAGTTTGATGATGATGAATTTGATGAAGGGTATGATTGGGATGATGAAAGTTTGGAGAATTTTGGGGATGCTGAAGTTTGTGACGAAAACAGTGAACAGAAAGAAGATGGAGTGAAGAATCATAAGTCGAACGATGCTGAATTGAAATCGAATTTGAGTGGACGAGAGAGGAGCATAAACATGAATTCCGTTCTGCACCCGGTAGAGAATCTTACTCAATGGAAAGCAATCAAAGCAAAAGTTGCATCAAGCAAACAAACGAGAAAGGAAAATGTTCCATCAGAGCAAAAAACAAGCATGCTGCCTGTAGTCTTGGAATCAAGCTCGAAGTTCTCGCCTTGCGTCTTGGAATCGAATGCCATTCAGTCGAAGCCTTTGTTGCAGGAAGTTGCAGTTGATGCTAGTCTTTCAAACTGGTTGGTTTCTCCTAGTTATAATGTCTCCACTACAATTCGTTGTCAATGA

Protein sequence

>MsG0180004049.01.T01

MGCFLQCFGLTSKRKRRKTLYKVLAGENQKYGNYQVLATITEKSIVPYSQIRGRDQEKEKSGVKSKKKKKVSFNLNVEMFEANPSSYQVLDDDDEEDENKETTVESEREGSVVSMIRYPSNHRYHNCSYDNEEEDEIVYEESDIDEFDDDEFDEGYDWDDESLENFGDAEVCDENSEQKEDGVKNHKSNDAELKSNLSGRERSINMNSVLHPVENLTQWKAIKAKVASSKQTRKENVPSEQKTSMLPVVLESSSKFSPCVLESNAIQSKPLLQEVAVDASLSNWLVSPSYNVSTTIRCQ*