AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280011014.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280011014.01.T01 MTR_2g096610 97.297 148 4 0 1 148 1 148 1.63e-100 285
MsG0280011014.01.T01 MTR_4g063280 90.909 132 11 1 18 148 14 145 1.42e-79 232
MsG0280011014.01.T01 MTR_4g071150 86.842 152 16 2 1 148 1 152 1.19e-78 229
MsG0280011014.01.T01 MTR_4g064005 87.333 150 15 2 1 148 1 148 4.73e-78 228
MsG0280011014.01.T01 MTR_2g096570 87.023 131 13 1 22 148 23 153 1.33e-76 224
MsG0280011014.01.T01 MTR_4g064967 91.339 127 9 1 22 148 24 148 1.56e-76 224
MsG0280011014.01.T01 MTR_4g063410 84.000 150 20 2 1 148 1 148 6.35e-75 220
MsG0280011014.01.T01 MTR_7g113160 81.061 132 25 0 9 140 13 144 2.37e-74 218
MsG0280011014.01.T01 MTR_8g086640 89.394 132 12 1 1 130 1 132 8.15e-72 212
MsG0280011014.01.T01 MTR_7g093910 73.810 126 31 1 2 127 4 127 1.10e-60 184
MsG0280011014.01.T01 MTR_7g108320 74.400 125 29 1 3 127 6 127 3.78e-60 182
MsG0280011014.01.T01 MTR_7g114060 73.810 126 29 2 3 127 2 124 1.26e-59 181
MsG0280011014.01.T01 MTR_7g114040 73.810 126 29 2 3 127 2 124 1.26e-59 181
MsG0280011014.01.T01 MTR_2g082510 78.378 111 24 0 17 127 11 121 5.33e-59 179
MsG0280011014.01.T01 MTR_2g082220 79.630 108 22 0 20 127 14 121 2.10e-58 177
MsG0280011014.01.T01 MTR_6g005150 65.354 127 30 3 22 147 26 139 1.78e-50 158
MsG0280011014.01.T01 MTR_7g114050 61.029 136 42 2 5 140 4 128 4.79e-47 149
MsG0280011014.01.T01 MTR_4g063300 74.286 105 22 1 43 147 10 109 1.96e-45 144
MsG0280011014.01.T01 MTR_1g011750 60.177 113 43 2 19 130 23 134 7.13e-37 123
MsG0280011014.01.T01 MTR_1g011850 60.177 113 43 2 19 130 23 134 7.13e-37 123
MsG0280011014.01.T01 MTR_1g114050 57.983 119 48 2 9 126 10 127 9.87e-37 123
MsG0280011014.01.T01 MTR_1g071040 62.500 104 37 2 24 126 28 130 2.69e-35 119
MsG0280011014.01.T01 MTR_1g069770 48.958 96 48 1 43 137 9 104 4.79e-25 92.4
MsG0280011014.01.T01 MTR_4g048490 43.810 105 30 2 22 126 26 101 8.85e-17 71.2
MsG0280011014.01.T01 MTR_7g010440 70.588 51 15 0 76 126 122 172 1.57e-16 74.7
MsG0280011014.01.T01 MTR_4g005910 74.419 43 11 0 85 127 4 46 2.38e-16 68.9
MsG0280011014.01.T01 MTR_7g027610 41.818 110 38 3 18 126 10 94 1.05e-15 68.9
MsG0280011014.01.T01 MTR_2g096590 75.000 40 10 0 108 147 12 51 6.71e-14 62.4
MsG0280011014.01.T01 MTR_0653s0030 54.545 55 25 0 53 107 43 97 3.58e-12 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280011014.01.T01 AT5G02560 82.353 153 22 2 1 148 1 153 9.70e-80 233
MsG0280011014.01.T01 AT5G27670 77.551 147 32 1 2 148 5 150 5.47e-76 223
MsG0280011014.01.T01 AT5G02560 71.186 177 22 3 1 148 1 177 9.82e-75 221
MsG0280011014.01.T01 AT5G59870 72.667 150 39 1 1 148 1 150 5.70e-73 215
MsG0280011014.01.T01 AT1G51060 80.180 111 22 0 17 127 11 121 2.38e-60 182
MsG0280011014.01.T01 AT1G08880 72.800 125 30 1 7 127 3 127 9.47e-59 179
MsG0280011014.01.T01 AT4G27230 77.477 111 25 0 17 127 11 121 1.33e-58 178
MsG0280011014.01.T01 AT4G27230 77.477 111 25 0 17 127 11 121 1.33e-58 178
MsG0280011014.01.T01 AT5G54640 78.378 111 24 0 17 127 11 121 1.71e-58 178
MsG0280011014.01.T01 AT1G54690 73.770 122 28 1 10 127 6 127 2.38e-58 178
MsG0280011014.01.T01 AT3G20670 77.477 111 25 0 17 127 11 121 2.95e-58 177
MsG0280011014.01.T01 AT2G38810 59.292 113 44 2 15 126 21 132 7.92e-37 123
MsG0280011014.01.T01 AT2G38810 59.292 113 44 2 15 126 21 132 7.92e-37 123
MsG0280011014.01.T01 AT2G38810 59.292 113 44 2 15 126 21 132 7.92e-37 123
MsG0280011014.01.T01 AT2G38810 59.292 113 44 2 15 126 21 132 7.92e-37 123
MsG0280011014.01.T01 AT1G52740 62.857 105 37 2 23 126 27 130 1.40e-35 120
MsG0280011014.01.T01 AT3G54560 58.716 109 43 2 23 130 29 136 1.98e-34 117
MsG0280011014.01.T01 AT3G54560 58.716 109 43 2 23 130 29 136 1.98e-34 117
MsG0280011014.01.T01 AT4G13570 48.913 92 45 2 30 120 28 118 1.70e-20 81.3
MsG0280011014.01.T01 AT4G13570 48.352 91 45 2 31 120 35 124 4.76e-20 80.5

Find 47 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTAAGCCTTCTTTGGAGATT+TGG 0.253652 2:-78354836 None:intergenic
GAAGCCTTCTCATTCTTCTT+AGG 0.258486 2:-78354784 None:intergenic
AACTAAATCTAAGCCTTCTT+TGG 0.259152 2:-78354844 None:intergenic
TTCTTCTTAGGTAAAAGAAT+TGG 0.279364 2:-78354772 None:intergenic
CTTGTCACCGCCTTCTTCCT+TGG 0.286829 2:-78354371 None:intergenic
ACCAGCTCCTTTCTTCGTCT+TGG 0.348361 2:-78354333 None:intergenic
GGTAGCCTTGGATGGAGATT+TGG 0.359896 2:-78354815 None:intergenic
AAAAGAATTGGGTTGATGTT+TGG 0.366899 2:-78354760 None:intergenic
CTTTGGAGATTTGGTAGCCT+TGG 0.379306 2:-78354827 None:intergenic
CAATTTCCAGTTGGAAGAAT+CGG 0.397925 2:+78354413 MsG0280011014.01.T01:CDS
AGAACAGCTGCAAGATAAAC+AGG 0.409350 2:-78354485 None:intergenic
TCTTCTTAGGTAAAAGAATT+GGG 0.411045 2:-78354771 None:intergenic
TTGTGTTCAGGTTCTTGAAT+TGG 0.420978 2:+78354609 MsG0280011014.01.T01:intron
TACCGACCGATTCTTCCAAC+TGG 0.449917 2:-78354419 None:intergenic
TACGCTCAGCGTGTTGGTAC+TGG 0.452334 2:+78354458 MsG0280011014.01.T01:CDS
CACGTGACAACAAGAAGAAC+AGG 0.464610 2:+78354644 MsG0280011014.01.T01:CDS
TGGAAATTGAAGACCAGCTC+TGG 0.468882 2:-78354399 None:intergenic
AAAGGAGCTGGTGGAAGGAA+AGG 0.477487 2:+78354344 MsG0280011014.01.T01:CDS
GCAAGCACCAAGACGAAGAA+AGG 0.482883 2:+78354326 MsG0280011014.01.T01:CDS
GTTCAGGTTCTTGAATTGGC+TGG 0.491391 2:+78354613 MsG0280011014.01.T01:intron
CATCCCTAGGCATTTGCTGT+TGG 0.495579 2:+78354669 MsG0280011014.01.T01:CDS
GAACTTGGAAAGTTGCTAGC+TGG 0.514674 2:+78354709 MsG0280011014.01.T01:CDS
GGCTACCAAATCTCCAAAGA+AGG 0.519492 2:+78354831 MsG0280011014.01.T01:CDS
GGAGATTTGGTAGCCTTGGA+TGG 0.524077 2:-78354823 None:intergenic
GTGAGGAATGATGAAGAACT+TGG 0.529342 2:+78354694 MsG0280011014.01.T01:CDS
ATCGGTCGGTACTTGAAGAA+AGG 0.536042 2:+78354431 MsG0280011014.01.T01:CDS
GAAAGGTGGTCCAAGGAAGA+AGG 0.553269 2:+78354361 MsG0280011014.01.T01:CDS
TTTACCTAAGAAGAATGAGA+AGG 0.555333 2:+78354780 MsG0280011014.01.T01:CDS
CGAAGAAAGGAGCTGGTGGA+AGG 0.557443 2:+78354339 MsG0280011014.01.T01:CDS
TCTTGAATATCTTGCTGCTG+AGG 0.561765 2:+78354505 MsG0280011014.01.T01:CDS
AAATTGAAGACCAGCTCTGG+TGG 0.562253 2:-78354396 None:intergenic
GTGACAAGATCCACCAGAGC+TGG 0.571539 2:+78354386 MsG0280011014.01.T01:CDS
ACTGCCAACAGCAAATGCCT+AGG 0.578012 2:-78354673 None:intergenic
ACCAAGACGAAGAAAGGAGC+TGG 0.579581 2:+78354332 MsG0280011014.01.T01:CDS
GGAAGATACGCTCAGCGTGT+TGG 0.585684 2:+78354452 MsG0280011014.01.T01:CDS
TGCTACCAAATCTCCATCCA+AGG 0.591170 2:+78354810 MsG0280011014.01.T01:CDS
GCTGGTCTTCAATTTCCAGT+TGG 0.598252 2:+78354404 MsG0280011014.01.T01:CDS
GTGGAAGGAAAGGTGGTCCA+AGG 0.602831 2:+78354354 MsG0280011014.01.T01:CDS
GGCATTTGCTGTTGGCAGTG+AGG 0.603106 2:+78354677 MsG0280011014.01.T01:CDS
CTGCCAACAGCAAATGCCTA+GGG 0.606589 2:-78354672 None:intergenic
AGAAGAACAGGATCATCCCT+AGG 0.619121 2:+78354656 MsG0280011014.01.T01:CDS
TTCCAGTTGGAAGAATCGGT+CGG 0.638316 2:+78354417 MsG0280011014.01.T01:CDS
GGAGCTGGTGGAAGGAAAGG+TGG 0.644393 2:+78354347 MsG0280011014.01.T01:CDS
AAGACGAAGAAAGGAGCTGG+TGG 0.662071 2:+78354335 MsG0280011014.01.T01:CDS
GCTGGAGTTACAATTGCACA+TGG 0.698871 2:+78354727 MsG0280011014.01.T01:CDS
AGGTGGTCCAAGGAAGAAGG+CGG 0.714301 2:+78354364 MsG0280011014.01.T01:CDS
GGAGTTACAATTGCACATGG+TGG 0.781867 2:+78354730 MsG0280011014.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTCAATTTCATCAAAATT+AGG - Chr2:78354584-78354603 None:intergenic 15.0%
!! TTTCAATTTCATCAAAATTA+GGG - Chr2:78354583-78354602 None:intergenic 15.0%
!! CAAATTTGAAAATCAAAAGA+GGG - Chr2:78354551-78354570 None:intergenic 20.0%
!! TCAAATTTGAAAATCAAAAG+AGG - Chr2:78354552-78354571 None:intergenic 20.0%
! TCTTCTTAGGTAAAAGAATT+GGG - Chr2:78354774-78354793 None:intergenic 25.0%
! TTCTTCTTAGGTAAAAGAAT+TGG - Chr2:78354775-78354794 None:intergenic 25.0%
!!! TTGAAATTTGATTTGTGTTC+AGG + Chr2:78354597-78354616 MsG0280011014.01.T01:intron 25.0%
TTTACCTAAGAAGAATGAGA+AGG + Chr2:78354780-78354799 MsG0280011014.01.T01:CDS 30.0%
!! AAAAGAATTGGGTTGATGTT+TGG - Chr2:78354763-78354782 None:intergenic 30.0%
CAATTTCCAGTTGGAAGAAT+CGG + Chr2:78354413-78354432 MsG0280011014.01.T01:CDS 35.0%
! TTGTGTTCAGGTTCTTGAAT+TGG + Chr2:78354609-78354628 MsG0280011014.01.T01:intron 35.0%
AGAACAGCTGCAAGATAAAC+AGG - Chr2:78354488-78354507 None:intergenic 40.0%
GAAGCCTTCTCATTCTTCTT+AGG - Chr2:78354787-78354806 None:intergenic 40.0%
GTGAGGAATGATGAAGAACT+TGG + Chr2:78354694-78354713 MsG0280011014.01.T01:CDS 40.0%
TCTTGAATATCTTGCTGCTG+AGG + Chr2:78354505-78354524 MsG0280011014.01.T01:CDS 40.0%
! CTAAGCCTTCTTTGGAGATT+TGG - Chr2:78354839-78354858 None:intergenic 40.0%
AGAAGAACAGGATCATCCCT+AGG + Chr2:78354656-78354675 MsG0280011014.01.T01:CDS 45.0%
ATCGGTCGGTACTTGAAGAA+AGG + Chr2:78354431-78354450 MsG0280011014.01.T01:CDS 45.0%
CACGTGACAACAAGAAGAAC+AGG + Chr2:78354644-78354663 MsG0280011014.01.T01:CDS 45.0%
GAACTTGGAAAGTTGCTAGC+TGG + Chr2:78354709-78354728 MsG0280011014.01.T01:CDS 45.0%
GCTGGAGTTACAATTGCACA+TGG + Chr2:78354727-78354746 MsG0280011014.01.T01:CDS 45.0%
GGAGTTACAATTGCACATGG+TGG + Chr2:78354730-78354749 MsG0280011014.01.T01:CDS 45.0%
GGCTACCAAATCTCCAAAGA+AGG + Chr2:78354831-78354850 MsG0280011014.01.T01:CDS 45.0%
TGCTACCAAATCTCCATCCA+AGG + Chr2:78354810-78354829 MsG0280011014.01.T01:CDS 45.0%
TGGAAATTGAAGACCAGCTC+TGG - Chr2:78354402-78354421 None:intergenic 45.0%
TTCCAGTTGGAAGAATCGGT+CGG + Chr2:78354417-78354436 MsG0280011014.01.T01:CDS 45.0%
! AAATTGAAGACCAGCTCTGG+TGG - Chr2:78354399-78354418 None:intergenic 45.0%
! CTTTGGAGATTTGGTAGCCT+TGG - Chr2:78354830-78354849 None:intergenic 45.0%
! GTTCAGGTTCTTGAATTGGC+TGG + Chr2:78354613-78354632 MsG0280011014.01.T01:intron 45.0%
!! GCTGGTCTTCAATTTCCAGT+TGG + Chr2:78354404-78354423 MsG0280011014.01.T01:CDS 45.0%
AAAGGAGCTGGTGGAAGGAA+AGG + Chr2:78354344-78354363 MsG0280011014.01.T01:CDS 50.0%
AAGACGAAGAAAGGAGCTGG+TGG + Chr2:78354335-78354354 MsG0280011014.01.T01:CDS 50.0%
ACCAAGACGAAGAAAGGAGC+TGG + Chr2:78354332-78354351 MsG0280011014.01.T01:CDS 50.0%
ACCAGCTCCTTTCTTCGTCT+TGG - Chr2:78354336-78354355 None:intergenic 50.0%
ACTGCCAACAGCAAATGCCT+AGG - Chr2:78354676-78354695 None:intergenic 50.0%
CTGCCAACAGCAAATGCCTA+GGG - Chr2:78354675-78354694 None:intergenic 50.0%
GAAAGGTGGTCCAAGGAAGA+AGG + Chr2:78354361-78354380 MsG0280011014.01.T01:CDS 50.0%
GCAAGCACCAAGACGAAGAA+AGG + Chr2:78354326-78354345 MsG0280011014.01.T01:CDS 50.0%
! GGAGATTTGGTAGCCTTGGA+TGG - Chr2:78354826-78354845 None:intergenic 50.0%
! GGTAGCCTTGGATGGAGATT+TGG - Chr2:78354818-78354837 None:intergenic 50.0%
! TACCGACCGATTCTTCCAAC+TGG - Chr2:78354422-78354441 None:intergenic 50.0%
!! CATCCCTAGGCATTTGCTGT+TGG + Chr2:78354669-78354688 MsG0280011014.01.T01:CDS 50.0%
AGGTGGTCCAAGGAAGAAGG+CGG + Chr2:78354364-78354383 MsG0280011014.01.T01:CDS 55.0%
CGAAGAAAGGAGCTGGTGGA+AGG + Chr2:78354339-78354358 MsG0280011014.01.T01:CDS 55.0%
CTTGTCACCGCCTTCTTCCT+TGG - Chr2:78354374-78354393 None:intergenic 55.0%
GGAAGATACGCTCAGCGTGT+TGG + Chr2:78354452-78354471 MsG0280011014.01.T01:CDS 55.0%
GTGACAAGATCCACCAGAGC+TGG + Chr2:78354386-78354405 MsG0280011014.01.T01:CDS 55.0%
GTGGAAGGAAAGGTGGTCCA+AGG + Chr2:78354354-78354373 MsG0280011014.01.T01:CDS 55.0%
! TACGCTCAGCGTGTTGGTAC+TGG + Chr2:78354458-78354477 MsG0280011014.01.T01:CDS 55.0%
!! GGCATTTGCTGTTGGCAGTG+AGG + Chr2:78354677-78354696 MsG0280011014.01.T01:CDS 55.0%
GGAGCTGGTGGAAGGAAAGG+TGG + Chr2:78354347-78354366 MsG0280011014.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr2 gene 78354320 78354858 78354320 ID=MsG0280011014.01;Name=MsG0280011014.01
Chr2 mRNA 78354320 78354858 78354320 ID=MsG0280011014.01.T01;Parent=MsG0280011014.01;Name=MsG0280011014.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|1|1|2|0|148
Chr2 exon 78354320 78354526 78354320 ID=MsG0280011014.01.T01:exon:20565;Parent=MsG0280011014.01.T01
Chr2 exon 78354619 78354858 78354619 ID=MsG0280011014.01.T01:exon:20564;Parent=MsG0280011014.01.T01
Chr2 CDS 78354320 78354526 78354320 ID=MsG0280011014.01.T01:cds;Parent=MsG0280011014.01.T01
Chr2 CDS 78354619 78354858 78354619 ID=MsG0280011014.01.T01:cds;Parent=MsG0280011014.01.T01
Gene Sequence

>MsG0280011014.01.T01

ATGGACGCAAGCACCAAGACGAAGAAAGGAGCTGGTGGAAGGAAAGGTGGTCCAAGGAAGAAGGCGGTGACAAGATCCACCAGAGCTGGTCTTCAATTTCCAGTTGGAAGAATCGGTCGGTACTTGAAGAAAGGAAGATACGCTCAGCGTGTTGGTACTGGTGCTCCTGTTTATCTTGCAGCTGTTCTTGAATATCTTGCTGCTGAGGTTCTTGAATTGGCTGGAAATGCTGCACGTGACAACAAGAAGAACAGGATCATCCCTAGGCATTTGCTGTTGGCAGTGAGGAATGATGAAGAACTTGGAAAGTTGCTAGCTGGAGTTACAATTGCACATGGTGGTGTTCTTCCAAACATCAACCCAATTCTTTTACCTAAGAAGAATGAGAAGGCTTCAACTGCTACCAAATCTCCATCCAAGGCTACCAAATCTCCAAAGAAGGCTTAG

Protein sequence

>MsG0280011014.01.T01

MDASTKTKKGAGGRKGGPRKKAVTRSTRAGLQFPVGRIGRYLKKGRYAQRVGTGAPVYLAAVLEYLAAEVLELAGNAARDNKKNRIIPRHLLLAVRNDEELGKLLAGVTIAHGGVLPNINPILLPKKNEKASTATKSPSKATKSPKKA*