AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780040071.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040071.01.T01 MTR_7g084150 98.592 213 3 0 10 222 61 273 2.97e-157 436
MsG0780040071.01.T01 MTR_3g082230 79.688 64 13 0 103 166 68 131 2.11e-28 106
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040071.01.T01 AT5G13430 83.410 217 36 0 6 222 56 272 5.13e-138 388
MsG0780040071.01.T01 AT5G13440 84.038 213 34 0 10 222 62 274 6.49e-138 387

Find 73 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGATCTCATTATGATATTTC+TGG 0.147844 7:+72731357 MsG0780040071.01.T01:CDS
GAAACAGTTGCAGGAATTTC+AGG 0.237158 7:-72730868 None:intergenic
ATGATATTTCTGGCAGAATT+AGG 0.242646 7:+72731367 MsG0780040071.01.T01:CDS
CCGATCACAACGAGCCATTC+TGG 0.268480 7:-72731261 None:intergenic
GGTACCAACTTATTCATTCT+TGG 0.306788 7:+72731416 MsG0780040071.01.T01:CDS
AACAGATGACGATGTGAAAT+TGG 0.317786 7:+72731176 MsG0780040071.01.T01:CDS
GATCGGTGTTTGCACACATC+TGG 0.323690 7:+72731278 MsG0780040071.01.T01:CDS
CTCCGAATTTCAAGTATGTT+TGG 0.327058 7:+72731598 MsG0780040071.01.T01:three_prime_UTR
TTCGCATACTTTGTCCTAAC+AGG 0.331271 7:+72730976 MsG0780040071.01.T01:CDS
ATCATATCATAAATTACATT+TGG 0.332883 7:+72731725 MsG0780040071.01.T01:three_prime_UTR
GGGACCTGCACCATACAATT+TGG 0.359660 7:+72731392 MsG0780040071.01.T01:CDS
CGCCAAACATACTTGAAATT+CGG 0.375232 7:-72731600 None:intergenic
TGCATTCCCTTGCCAAATGC+TGG 0.381560 7:+72731303 MsG0780040071.01.T01:CDS
CCGCCAAAGTCTCCAGCATT+TGG 0.391956 7:-72731315 None:intergenic
TTAACAGCAGAAACAGTTGC+AGG 0.410008 7:-72730877 None:intergenic
TCATACACAATCTTTGAAGA+TGG 0.414369 7:-72730904 None:intergenic
AACAAATAATAAAGATTCTC+AGG 0.415954 7:-72731551 None:intergenic
TTGCCAAATGCTGGAGACTT+TGG 0.416842 7:+72731312 MsG0780040071.01.T01:CDS
GCCCTCTTGCTTGGATCACC+AGG 0.425512 7:-72730952 None:intergenic
GTTGATCGGTTAAAGACCTT+GGG 0.438975 7:+72731452 MsG0780040071.01.T01:exon
TAAAAGCTGATGATGACTTC+AGG 0.441229 7:+72731679 MsG0780040071.01.T01:three_prime_UTR
TATGCGAATGCCCTCTTGCT+TGG 0.442545 7:-72730961 None:intergenic
AAACAAACCTTCCACCTGTT+AGG 0.452175 7:-72730990 None:intergenic
CCAAATGCTGGAGACTTTGG+CGG 0.457248 7:+72731315 MsG0780040071.01.T01:CDS
GTGGAAAGCCAGTATTCATC+AGG 0.460050 7:+72731148 MsG0780040071.01.T01:CDS
ATTGTGCGTATGCTATTCGT+TGG 0.461507 7:+72730699 None:intergenic
CGATCACAACGAGCCATTCT+GGG 0.462447 7:-72731260 None:intergenic
GCTCAGCATGTCCGCAAGTA+AGG 0.463109 7:+72731050 MsG0780040071.01.T01:CDS
ACCTCCAAATTGTATGGTGC+AGG 0.482330 7:-72731396 None:intergenic
GTTCTGCGCCTGATGAATAC+TGG 0.484039 7:-72731156 None:intergenic
CAAACTTGAGGACAAGTAAA+CGG 0.484302 7:-72731026 None:intergenic
TGTTGATCGGTTAAAGACCT+TGG 0.487515 7:+72731451 MsG0780040071.01.T01:exon
CACCAGGAGGAAACCGTTCA+TGG 0.490179 7:-72730936 None:intergenic
GTTGGTACCTCCAAATTGTA+TGG 0.490642 7:-72731402 None:intergenic
AGTAGTGCCTGGCTCAATGC+TGG 0.496319 7:-72731109 None:intergenic
ACCAACTTATTCATTCTTGG+AGG 0.499770 7:+72731419 MsG0780040071.01.T01:CDS
AAATGCGCCATGGACATACT+TGG 0.503500 7:+72731528 MsG0780040071.01.T01:three_prime_UTR
TCCTCCAAGAATGAATAAGT+TGG 0.514284 7:-72731420 None:intergenic
AAGTAAGGATGTTCTTGCTA+TGG 0.519506 7:+72731065 MsG0780040071.01.T01:CDS
AACCATGAACGGTTTCCTCC+TGG 0.520987 7:+72730934 MsG0780040071.01.T01:CDS
GAGGAAAACAAGTTGTTGAT+CGG 0.524976 7:+72731438 MsG0780040071.01.T01:CDS
CCAGAATGGCTCGTTGTGAT+CGG 0.536344 7:+72731261 MsG0780040071.01.T01:CDS
ATCGGTGTTTGCACACATCT+GGG 0.542075 7:+72731279 MsG0780040071.01.T01:CDS
GATCTGTCCAGCATTGAGCC+AGG 0.548945 7:+72731102 MsG0780040071.01.T01:CDS
GCACTACTGTCACTGTTAAG+TGG 0.549316 7:+72731124 MsG0780040071.01.T01:CDS
TATTTCTGGCAGAATTAGGA+AGG 0.553507 7:+72731371 MsG0780040071.01.T01:CDS
GACCTTGGGCTGCTCACTAG+TGG 0.554881 7:+72731466 MsG0780040071.01.T01:three_prime_UTR
CTCTCTCAGAATCTTGCTGA+GGG 0.558977 7:-72731230 None:intergenic
ATGCTGGAGACTTTGGCGGA+TGG 0.561425 7:+72731319 MsG0780040071.01.T01:CDS
TTGGCAAACAGTGTTGATGT+TGG 0.567703 7:+72731195 MsG0780040071.01.T01:CDS
ACTCTCTCAGAATCTTGCTG+AGG 0.573430 7:-72731231 None:intergenic
TTGTGCGTATGCTATTCGTT+GGG 0.585841 7:+72730700 None:intergenic
TTAACAGTGACAGTAGTGCC+TGG 0.588903 7:-72731120 None:intergenic
GCATACTTTGTCCTAACAGG+TGG 0.589727 7:+72730979 MsG0780040071.01.T01:CDS
ACTGTCACTGTTAAGTGGCG+TGG 0.593737 7:+72731129 MsG0780040071.01.T01:CDS
TCATAATGAGATCCATGACA+TGG 0.597841 7:-72731348 None:intergenic
TTCCACTAGTGAGCAGCCCA+AGG 0.599328 7:-72731468 None:intergenic
TCTCAGGCCAAGTATGTCCA+TGG 0.606329 7:-72731535 None:intergenic
AAAGTCTCCAGCATTTGGCA+AGG 0.607108 7:-72731310 None:intergenic
ACTTTGTCCTAACAGGTGGA+AGG 0.607644 7:+72730983 MsG0780040071.01.T01:CDS
AGAGAGTCAAGAACCCAGAA+TGG 0.614301 7:+72731247 MsG0780040071.01.T01:CDS
ATGATGAACACAACCATGAA+CGG 0.617851 7:+72730923 MsG0780040071.01.T01:CDS
ACCTGCACCATACAATTTGG+AGG 0.619569 7:+72731395 MsG0780040071.01.T01:CDS
GATAATGTCGAAATGCGCCA+TGG 0.622811 7:+72731518 MsG0780040071.01.T01:three_prime_UTR
ACATGCTGAGCACAAACTTG+AGG 0.627184 7:-72731038 None:intergenic
AGCAAGAACATCCTTACTTG+CGG 0.635054 7:-72731061 None:intergenic
AAGTCTCCAGCATTTGGCAA+GGG 0.635128 7:-72731309 None:intergenic
ATTTCTGGCAGAATTAGGAA+GGG 0.638657 7:+72731372 MsG0780040071.01.T01:CDS
CTCCTGGTGATCCAAGCAAG+AGG 0.642258 7:+72730950 MsG0780040071.01.T01:CDS
TCCTGGTGATCCAAGCAAGA+GGG 0.654973 7:+72730951 MsG0780040071.01.T01:CDS
CATAATGAGATCCATGACAT+GGG 0.657346 7:-72731347 None:intergenic
GACAAGTAAACGGATCAAAG+AGG 0.665826 7:-72731016 None:intergenic
CTCTTGCTTGGATCACCAGG+AGG 0.672619 7:-72730949 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATCAAATTAAATCAATTAA+TGG - Chr7:72730793-72730812 None:intergenic 10.0%
!!! ATTTATATTTTTATCTAACT+AGG + Chr7:72730760-72730779 MsG0780040071.01.T01:intron 10.0%
!! ATCATATCATAAATTACATT+TGG + Chr7:72731725-72731744 MsG0780040071.01.T01:three_prime_UTR 15.0%
!!! CCAAAATATTTTTGATTATT+TGG + Chr7:72731496-72731515 MsG0780040071.01.T01:three_prime_UTR 15.0%
!!! CCAAATAATCAAAAATATTT+TGG - Chr7:72731499-72731518 None:intergenic 15.0%
!! AAATTGTAAACAAAAAAGTC+AGG - Chr7:72731645-72731664 None:intergenic 20.0%
!! AACAAATAATAAAGATTCTC+AGG - Chr7:72731554-72731573 None:intergenic 20.0%
GGATCTCATTATGATATTTC+TGG + Chr7:72731357-72731376 MsG0780040071.01.T01:CDS 30.0%
! TCATACACAATCTTTGAAGA+TGG - Chr7:72730907-72730926 None:intergenic 30.0%
!! ATGATATTTCTGGCAGAATT+AGG + Chr7:72731367-72731386 MsG0780040071.01.T01:CDS 30.0%
!! GATTTAATTTGATTGCATCC+AGG + Chr7:72730799-72730818 MsG0780040071.01.T01:intron 30.0%
!!! TAATGCTGTTTTCTTTTGTG+TGG - Chr7:72730846-72730865 None:intergenic 30.0%
AACAGATGACGATGTGAAAT+TGG + Chr7:72731176-72731195 MsG0780040071.01.T01:CDS 35.0%
AAGTAAGGATGTTCTTGCTA+TGG + Chr7:72731065-72731084 MsG0780040071.01.T01:CDS 35.0%
ACCAACTTATTCATTCTTGG+AGG + Chr7:72731419-72731438 MsG0780040071.01.T01:CDS 35.0%
ATGATGAACACAACCATGAA+CGG + Chr7:72730923-72730942 MsG0780040071.01.T01:CDS 35.0%
CAAACTTGAGGACAAGTAAA+CGG - Chr7:72731029-72731048 None:intergenic 35.0%
CATAATGAGATCCATGACAT+GGG - Chr7:72731350-72731369 None:intergenic 35.0%
CGCCAAACATACTTGAAATT+CGG - Chr7:72731603-72731622 None:intergenic 35.0%
GAGGAAAACAAGTTGTTGAT+CGG + Chr7:72731438-72731457 MsG0780040071.01.T01:CDS 35.0%
GGTACCAACTTATTCATTCT+TGG + Chr7:72731416-72731435 MsG0780040071.01.T01:CDS 35.0%
TCATAATGAGATCCATGACA+TGG - Chr7:72731351-72731370 None:intergenic 35.0%
TCCTCCAAGAATGAATAAGT+TGG - Chr7:72731423-72731442 None:intergenic 35.0%
! CTCCGAATTTCAAGTATGTT+TGG + Chr7:72731598-72731617 MsG0780040071.01.T01:three_prime_UTR 35.0%
! TAAAAGCTGATGATGACTTC+AGG + Chr7:72731679-72731698 MsG0780040071.01.T01:three_prime_UTR 35.0%
!! ATTTCTGGCAGAATTAGGAA+GGG + Chr7:72731372-72731391 MsG0780040071.01.T01:CDS 35.0%
!! TATTTCTGGCAGAATTAGGA+AGG + Chr7:72731371-72731390 MsG0780040071.01.T01:CDS 35.0%
AAACAAACCTTCCACCTGTT+AGG - Chr7:72730993-72731012 None:intergenic 40.0%
AGCAAGAACATCCTTACTTG+CGG - Chr7:72731064-72731083 None:intergenic 40.0%
GAAACAGTTGCAGGAATTTC+AGG - Chr7:72730871-72730890 None:intergenic 40.0%
GACAAGTAAACGGATCAAAG+AGG - Chr7:72731019-72731038 None:intergenic 40.0%
GTTGATCGGTTAAAGACCTT+GGG + Chr7:72731452-72731471 MsG0780040071.01.T01:exon 40.0%
GTTGGTACCTCCAAATTGTA+TGG - Chr7:72731405-72731424 None:intergenic 40.0%
TGTTGATCGGTTAAAGACCT+TGG + Chr7:72731451-72731470 MsG0780040071.01.T01:exon 40.0%
TTAACAGCAGAAACAGTTGC+AGG - Chr7:72730880-72730899 None:intergenic 40.0%
TTCGCATACTTTGTCCTAAC+AGG + Chr7:72730976-72730995 MsG0780040071.01.T01:CDS 40.0%
! TTGGGTTGTGTGTTAGTATG+AGG + Chr7:72730718-72730737 MsG0780040071.01.T01:intron 40.0%
!! TTGGCAAACAGTGTTGATGT+TGG + Chr7:72731195-72731214 MsG0780040071.01.T01:CDS 40.0%
AAAGTCTCCAGCATTTGGCA+AGG - Chr7:72731313-72731332 None:intergenic 45.0%
AAATGCGCCATGGACATACT+TGG + Chr7:72731528-72731547 MsG0780040071.01.T01:three_prime_UTR 45.0%
AAGTCTCCAGCATTTGGCAA+GGG - Chr7:72731312-72731331 None:intergenic 45.0%
ACATGCTGAGCACAAACTTG+AGG - Chr7:72731041-72731060 None:intergenic 45.0%
ACCTCCAAATTGTATGGTGC+AGG - Chr7:72731399-72731418 None:intergenic 45.0%
ACCTGCACCATACAATTTGG+AGG + Chr7:72731395-72731414 MsG0780040071.01.T01:CDS 45.0%
ACTCTCTCAGAATCTTGCTG+AGG - Chr7:72731234-72731253 None:intergenic 45.0%
ACTTTGTCCTAACAGGTGGA+AGG + Chr7:72730983-72731002 MsG0780040071.01.T01:CDS 45.0%
AGAGAGTCAAGAACCCAGAA+TGG + Chr7:72731247-72731266 MsG0780040071.01.T01:CDS 45.0%
AGTGACTCAGTAGCAAAACC+TGG - Chr7:72730820-72730839 None:intergenic 45.0%
CTCTCTCAGAATCTTGCTGA+GGG - Chr7:72731233-72731252 None:intergenic 45.0%
GATAATGTCGAAATGCGCCA+TGG + Chr7:72731518-72731537 MsG0780040071.01.T01:three_prime_UTR 45.0%
GCACTACTGTCACTGTTAAG+TGG + Chr7:72731124-72731143 MsG0780040071.01.T01:CDS 45.0%
GCATACTTTGTCCTAACAGG+TGG + Chr7:72730979-72730998 MsG0780040071.01.T01:CDS 45.0%
GTGGAAAGCCAGTATTCATC+AGG + Chr7:72731148-72731167 MsG0780040071.01.T01:CDS 45.0%
TTAACAGTGACAGTAGTGCC+TGG - Chr7:72731123-72731142 None:intergenic 45.0%
TTGCCAAATGCTGGAGACTT+TGG + Chr7:72731312-72731331 MsG0780040071.01.T01:CDS 45.0%
! ATCGGTGTTTGCACACATCT+GGG + Chr7:72731279-72731298 MsG0780040071.01.T01:CDS 45.0%
AACCATGAACGGTTTCCTCC+TGG + Chr7:72730934-72730953 MsG0780040071.01.T01:CDS 50.0%
ACTGTCACTGTTAAGTGGCG+TGG + Chr7:72731129-72731148 MsG0780040071.01.T01:CDS 50.0%
CCAAATGCTGGAGACTTTGG+CGG + Chr7:72731315-72731334 MsG0780040071.01.T01:CDS 50.0%
CCAGAATGGCTCGTTGTGAT+CGG + Chr7:72731261-72731280 MsG0780040071.01.T01:CDS 50.0%
CGATCACAACGAGCCATTCT+GGG - Chr7:72731263-72731282 None:intergenic 50.0%
GGGACCTGCACCATACAATT+TGG + Chr7:72731392-72731411 MsG0780040071.01.T01:CDS 50.0%
GTTCTGCGCCTGATGAATAC+TGG - Chr7:72731159-72731178 None:intergenic 50.0%
TATGCGAATGCCCTCTTGCT+TGG - Chr7:72730964-72730983 None:intergenic 50.0%
TCCTGGTGATCCAAGCAAGA+GGG + Chr7:72730951-72730970 MsG0780040071.01.T01:CDS 50.0%
TCTCAGGCCAAGTATGTCCA+TGG - Chr7:72731538-72731557 None:intergenic 50.0%
TGCATTCCCTTGCCAAATGC+TGG + Chr7:72731303-72731322 MsG0780040071.01.T01:CDS 50.0%
! GATCGGTGTTTGCACACATC+TGG + Chr7:72731278-72731297 MsG0780040071.01.T01:CDS 50.0%
!! GGATGGTTTTGCCCATGTCA+TGG + Chr7:72731336-72731355 MsG0780040071.01.T01:CDS 50.0%
AGTAGTGCCTGGCTCAATGC+TGG - Chr7:72731112-72731131 None:intergenic 55.0%
ATGCTGGAGACTTTGGCGGA+TGG + Chr7:72731319-72731338 MsG0780040071.01.T01:CDS 55.0%
CACCAGGAGGAAACCGTTCA+TGG - Chr7:72730939-72730958 None:intergenic 55.0%
CCGATCACAACGAGCCATTC+TGG - Chr7:72731264-72731283 None:intergenic 55.0%
CCGCCAAAGTCTCCAGCATT+TGG - Chr7:72731318-72731337 None:intergenic 55.0%
CTCCTGGTGATCCAAGCAAG+AGG + Chr7:72730950-72730969 MsG0780040071.01.T01:CDS 55.0%
CTCTTGCTTGGATCACCAGG+AGG - Chr7:72730952-72730971 None:intergenic 55.0%
GCTCAGCATGTCCGCAAGTA+AGG + Chr7:72731050-72731069 MsG0780040071.01.T01:CDS 55.0%
TTCCACTAGTGAGCAGCCCA+AGG - Chr7:72731471-72731490 None:intergenic 55.0%
! GATCTGTCCAGCATTGAGCC+AGG + Chr7:72731102-72731121 MsG0780040071.01.T01:CDS 55.0%
GACCTTGGGCTGCTCACTAG+TGG + Chr7:72731466-72731485 MsG0780040071.01.T01:three_prime_UTR 60.0%
GCCCTCTTGCTTGGATCACC+AGG - Chr7:72730955-72730974 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr7 gene 72730708 72731757 72730708 ID=MsG0780040071.01;Name=MsG0780040071.01
Chr7 mRNA 72730708 72731757 72730708 ID=MsG0780040071.01.T01;Parent=MsG0780040071.01;Name=MsG0780040071.01.T01;_AED=0.49;_eAED=0.51;_QI=0|0|0.5|1|1|1|2|293|222
Chr7 exon 72730708 72730732 72730708 ID=MsG0780040071.01.T01:exon:12897;Parent=MsG0780040071.01.T01
Chr7 exon 72730821 72731757 72730821 ID=MsG0780040071.01.T01:exon:12898;Parent=MsG0780040071.01.T01
Chr7 CDS 72730708 72730732 72730708 ID=MsG0780040071.01.T01:cds;Parent=MsG0780040071.01.T01
Chr7 CDS 72730821 72731464 72730821 ID=MsG0780040071.01.T01:cds;Parent=MsG0780040071.01.T01
Chr7 three_prime_UTR 72731465 72731757 72731465 ID=MsG0780040071.01.T01:three_prime_utr;Parent=MsG0780040071.01.T01
Gene Sequence

>MsG0780040071.01.T01

ATGCTATTCGTTGGGTTGTGTGTTAGTTTTGCTACTGAGTCACTGATCCACACAAAAGAAAACAGCATTATTCCTGAAATTCCTGCAACTGTTTCTGCTGTTAAAAATCCATCTTCAAAGATTGTGTATGATGAACACAACCATGAACGGTTTCCTCCTGGTGATCCAAGCAAGAGGGCATTCGCATACTTTGTCCTAACAGGTGGAAGGTTTGTTTATGCCTCTTTGATCCGTTTACTTGTCCTCAAGTTTGTGCTCAGCATGTCCGCAAGTAAGGATGTTCTTGCTATGGCTTCACTTGAAGTTGATCTGTCCAGCATTGAGCCAGGCACTACTGTCACTGTTAAGTGGCGTGGAAAGCCAGTATTCATCAGGCGCAGAACAGATGACGATGTGAAATTGGCAAACAGTGTTGATGTTGGATCTCTTCGTGACCCTCAGCAAGATTCTGAGAGAGTCAAGAACCCAGAATGGCTCGTTGTGATCGGTGTTTGCACACATCTGGGTTGCATTCCCTTGCCAAATGCTGGAGACTTTGGCGGATGGTTTTGCCCATGTCATGGATCTCATTATGATATTTCTGGCAGAATTAGGAAGGGACCTGCACCATACAATTTGGAGGTACCAACTTATTCATTCTTGGAGGAAAACAAGTTGTTGATCGGTTAA

Protein sequence

>MsG0780040071.01.T01

MLFVGLCVSFATESLIHTKENSIIPEIPATVSAVKNPSSKIVYDEHNHERFPPGDPSKRAFAYFVLTGGRFVYASLIRLLVLKFVLSMSASKDVLAMASLEVDLSSIEPGTTVTVKWRGKPVFIRRRTDDDVKLANSVDVGSLRDPQQDSERVKNPEWLVVIGVCTHLGCIPLPNAGDFGGWFCPCHGSHYDISGRIRKGPAPYNLEVPTYSFLEENKLLIG*