Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040521.01.T01 | XP_003625156.1 | 97.5 | 80 | 2 | 0 | 1 | 80 | 679 | 758 | 2.14E-50 | 179 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040521.01.T01 | P93756 | 65.574 | 61 | 21 | 0 | 3 | 63 | 684 | 744 | 2.37E-20 | 86.3 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040521.01.T01 | A2Q3L3 | 97.500 | 80 | 2 | 0 | 1 | 80 | 679 | 758 | 1.02e-50 | 179 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000145.01 | MsG0780040521.01 | 0.801124 | 1.043982e-48 | 4.946516e-46 |
| MsG0180000835.01 | MsG0780040521.01 | 0.800041 | 1.735601e-48 | 8.000356e-46 |
| MsG0780040521.01 | MsG0780041440.01 | 0.823205 | 1.569889e-53 | 1.342868e-50 |
| MsG0780040521.01 | MsG0880043937.01 | 0.801242 | 9.877207e-49 | 4.693834e-46 |
| MsG0480023172.01 | MsG0780040521.01 | 0.801666 | 8.086077e-49 | 3.883812e-46 |
| MsG0580025661.01 | MsG0780040521.01 | 0.800761 | 1.238516e-48 | 5.814087e-46 |
| MsG0680030413.01 | MsG0780040521.01 | 0.802981 | 4.333691e-49 | 2.152340e-46 |
| MsG0280008457.01 | MsG0780040521.01 | 0.809278 | 2.047773e-50 | 1.197218e-47 |
| MsG0280010148.01 | MsG0780040521.01 | 0.801359 | 9.347267e-49 | 4.455073e-46 |
| MsG0780037054.01 | MsG0780040521.01 | 0.817453 | 3.272147e-52 | 2.384043e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040521.01.T01 | MTR_7g092050 | 97.500 | 80 | 2 | 0 | 1 | 80 | 679 | 758 | 2.59e-54 | 179 |
| MsG0780040521.01.T01 | MTR_2g089440 | 45.000 | 60 | 33 | 0 | 3 | 62 | 492 | 551 | 1.15e-15 | 70.1 |
| MsG0780040521.01.T01 | MTR_2g089440 | 45.000 | 60 | 33 | 0 | 3 | 62 | 724 | 783 | 1.17e-15 | 70.1 |
| MsG0780040521.01.T01 | MTR_2g089360 | 44.444 | 72 | 38 | 1 | 3 | 72 | 719 | 790 | 1.63e-15 | 69.7 |
| MsG0780040521.01.T01 | MTR_0280s0040 | 44.444 | 63 | 35 | 0 | 3 | 65 | 717 | 779 | 5.04e-14 | 65.5 |
| MsG0780040521.01.T01 | MTR_1g099400 | 46.774 | 62 | 31 | 1 | 3 | 62 | 746 | 807 | 1.21e-12 | 61.6 |
| MsG0780040521.01.T01 | MTR_5g013070 | 37.681 | 69 | 43 | 0 | 1 | 69 | 735 | 803 | 9.32e-12 | 58.9 |
| MsG0780040521.01.T01 | MTR_6g043510 | 35.484 | 62 | 39 | 1 | 2 | 62 | 712 | 773 | 2.90e-11 | 57.8 |
| MsG0780040521.01.T01 | MTR_2g036490 | 39.344 | 61 | 37 | 0 | 18 | 78 | 372 | 432 | 5.05e-11 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040521.01.T01 | AT2G41890 | 65.574 | 61 | 21 | 0 | 3 | 63 | 684 | 744 | 2.42e-21 | 86.3 |
| MsG0780040521.01.T01 | AT2G19130 | 46.774 | 62 | 32 | 1 | 2 | 62 | 717 | 778 | 6.48e-15 | 68.2 |
| MsG0780040521.01.T01 | AT5G24080 | 45.902 | 61 | 32 | 1 | 3 | 62 | 627 | 687 | 1.54e-11 | 58.5 |
| MsG0780040521.01.T01 | AT5G24080 | 45.902 | 61 | 32 | 1 | 3 | 62 | 627 | 687 | 1.54e-11 | 58.5 |
| MsG0780040521.01.T01 | AT5G24080 | 45.902 | 61 | 32 | 1 | 3 | 62 | 754 | 814 | 1.74e-11 | 58.5 |
Find 22 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCATTTGATCCACAAGAAAA+TGG | 0.231856 | 7:-78731889 | MsG0780040521.01.T01:CDS |
| AATTGTCGGTGGTGCTGATT+TGG | 0.344236 | 7:-78732049 | MsG0780040521.01.T01:CDS |
| GCTCATTAAGAATCGCATTC+TGG | 0.405745 | 7:-78732014 | MsG0780040521.01.T01:CDS |
| GGATCAAATGGCAAATGAAA+TGG | 0.448307 | 7:+78731901 | None:intergenic |
| CTCACTACCTCTTCCATTGG+AGG | 0.477198 | 7:+78731964 | None:intergenic |
| ACAAAGAATGGATGGAAGGG+AGG | 0.479441 | 7:-78732095 | None:intergenic |
| GGAAGGGAGGGTAGCTAATG+TGG | 0.482621 | 7:-78732082 | MsG0780040521.01.T01:CDS |
| AAATGAAATGGAGGTGGTGG+TGG | 0.509860 | 7:+78731913 | None:intergenic |
| GGCAAATGAAATGGAGGTGG+TGG | 0.515777 | 7:+78731910 | None:intergenic |
| ACTCTCACTACCTCTTCCAT+TGG | 0.516459 | 7:+78731961 | None:intergenic |
| GTGGTTGATAAAAGAATTGT+CGG | 0.524081 | 7:-78732063 | MsG0780040521.01.T01:CDS |
| TGTCGGTGGTGCTGATTTGG+AGG | 0.538846 | 7:-78732046 | MsG0780040521.01.T01:CDS |
| CAAAGAATGGATGGAAGGGA+GGG | 0.559758 | 7:-78732094 | None:intergenic |
| TAGAAGACCTCCAATGGAAG+AGG | 0.560698 | 7:-78731971 | MsG0780040521.01.T01:CDS |
| TGAACATAGAAGACCTCCAA+TGG | 0.564528 | 7:-78731977 | MsG0780040521.01.T01:CDS |
| GAGGTAGTGAGAGTACTAGA+TGG | 0.564811 | 7:-78731952 | MsG0780040521.01.T01:CDS |
| GGATACAAAGAATGGATGGA+AGG | 0.567341 | 7:-78732099 | None:intergenic |
| TGGTTCAGAAACAGAATCAG+TGG | 0.601093 | 7:-78731869 | MsG0780040521.01.T01:CDS |
| GATACAAAGAATGGATGGAA+GGG | 0.618765 | 7:-78732098 | None:intergenic |
| AATGGCAAATGAAATGGAGG+TGG | 0.680391 | 7:+78731907 | None:intergenic |
| TCAAATGGCAAATGAAATGG+AGG | 0.688108 | 7:+78731904 | None:intergenic |
| GTTGATAAAAGAATTGTCGG+TGG | 0.757626 | 7:-78732060 | MsG0780040521.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTGGTTGATAAAAGAATTGT+CGG | - | Chr7:78731885-78731904 | MsG0780040521.01.T01:CDS | 30.0% |
| CCATTTGATCCACAAGAAAA+TGG | - | Chr7:78732059-78732078 | MsG0780040521.01.T01:CDS | 35.0% | |
| GGATCAAATGGCAAATGAAA+TGG | + | Chr7:78732050-78732069 | None:intergenic | 35.0% | |
| TCAAATGGCAAATGAAATGG+AGG | + | Chr7:78732047-78732066 | None:intergenic | 35.0% | |
| ! | CCATTTTCTTGTGGATCAAA+TGG | + | Chr7:78732062-78732081 | None:intergenic | 35.0% |
| ! | GTTGATAAAAGAATTGTCGG+TGG | - | Chr7:78731888-78731907 | MsG0780040521.01.T01:CDS | 35.0% |
| ! | GTTTCTGAACCATTTTCTTG+TGG | + | Chr7:78732071-78732090 | None:intergenic | 35.0% |
| AATGGCAAATGAAATGGAGG+TGG | + | Chr7:78732044-78732063 | None:intergenic | 40.0% | |
| GCTCATTAAGAATCGCATTC+TGG | - | Chr7:78731934-78731953 | MsG0780040521.01.T01:CDS | 40.0% | |
| TGAACATAGAAGACCTCCAA+TGG | - | Chr7:78731971-78731990 | MsG0780040521.01.T01:CDS | 40.0% | |
| TGGTTCAGAAACAGAATCAG+TGG | - | Chr7:78732079-78732098 | MsG0780040521.01.T01:CDS | 40.0% | |
| ACTCTCACTACCTCTTCCAT+TGG | + | Chr7:78731990-78732009 | None:intergenic | 45.0% | |
| TAGAAGACCTCCAATGGAAG+AGG | - | Chr7:78731977-78731996 | MsG0780040521.01.T01:CDS | 45.0% | |
| ! | AAATGAAATGGAGGTGGTGG+TGG | + | Chr7:78732038-78732057 | None:intergenic | 45.0% |
| !! | AATTGTCGGTGGTGCTGATT+TGG | - | Chr7:78731899-78731918 | MsG0780040521.01.T01:CDS | 45.0% |
| !! | GAGGTAGTGAGAGTACTAGA+TGG | - | Chr7:78731996-78732015 | MsG0780040521.01.T01:CDS | 45.0% |
| CTCACTACCTCTTCCATTGG+AGG | + | Chr7:78731987-78732006 | None:intergenic | 50.0% | |
| GGCAAATGAAATGGAGGTGG+TGG | + | Chr7:78732041-78732060 | None:intergenic | 50.0% | |
| GGAAGGGAGGGTAGCTAATG+TGG | - | Chr7:78731866-78731885 | MsG0780040521.01.T01:CDS | 55.0% | |
| !! | TGTCGGTGGTGCTGATTTGG+AGG | - | Chr7:78731902-78731921 | MsG0780040521.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 78731864 | 78732106 | 78731864 | ID=MsG0780040521.01;Name=MsG0780040521.01 |
| Chr7 | mRNA | 78731864 | 78732106 | 78731864 | ID=MsG0780040521.01.T01;Parent=MsG0780040521.01;Name=MsG0780040521.01.T01;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|80 |
| Chr7 | exon | 78731864 | 78732106 | 78731864 | ID=MsG0780040521.01.T01:exon:16385;Parent=MsG0780040521.01.T01 |
| Chr7 | CDS | 78731864 | 78732106 | 78731864 | ID=MsG0780040521.01.T01:cds;Parent=MsG0780040521.01.T01 |
| Gene Sequence |
| Protein sequence |
