AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880043937.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043937.01.T01 MTR_8g045300 95.541 157 7 0 1 157 1 157 2.32e-110 310
MsG0880043937.01.T01 MTR_8g045520 73.718 156 41 0 1 156 1 156 1.11e-84 245
MsG0880043937.01.T01 MTR_8g045490 68.590 156 48 1 1 156 1 155 3.09e-81 237
MsG0880043937.01.T01 MTR_8g045570 70.199 151 45 0 1 151 1 151 1.24e-80 235
MsG0880043937.01.T01 MTR_8g045400 67.308 156 50 1 1 156 1 155 1.12e-77 228
MsG0880043937.01.T01 MTR_8g045555 68.212 151 48 0 1 151 1 151 2.64e-77 227
MsG0880043937.01.T01 MTR_8g045695 65.385 156 53 1 1 156 1 155 2.85e-76 224
MsG0880043937.01.T01 MTR_8g045735 65.806 155 52 1 1 155 1 154 2.87e-74 219
MsG0880043937.01.T01 MTR_8g045640 62.821 156 57 1 1 156 1 155 1.70e-70 209
MsG0880043937.01.T01 MTR_8g045560 62.179 156 58 1 1 156 1 155 2.07e-69 207
MsG0880043937.01.T01 MTR_8g045665 61.538 156 59 1 1 156 1 155 2.97e-69 206
MsG0880043937.01.T01 MTR_1g030810 42.208 154 86 2 1 152 1 153 1.32e-38 129
MsG0880043937.01.T01 MTR_1g030820 39.610 154 90 2 1 152 1 153 4.44e-37 125
MsG0880043937.01.T01 MTR_8g012550 33.333 150 97 3 6 154 8 155 1.61e-26 98.2
MsG0880043937.01.T01 MTR_1g030840 32.692 156 97 4 1 149 1 155 4.80e-22 87.0
MsG0880043937.01.T01 MTR_1g031640 34.694 147 87 5 7 151 7 146 6.11e-21 83.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043937.01.T01 AT5G28010 32.895 152 99 3 3 152 15 165 7.23e-23 89.4
MsG0880043937.01.T01 AT5G28010 32.895 152 99 3 3 152 15 165 7.23e-23 89.4
MsG0880043937.01.T01 AT1G70830 32.237 152 100 3 3 152 22 172 1.27e-22 88.6
MsG0880043937.01.T01 AT1G70830 32.237 152 100 3 3 152 22 172 2.95e-22 88.6
MsG0880043937.01.T01 AT1G70840 32.237 152 100 3 3 152 20 170 6.25e-22 87.0
MsG0880043937.01.T01 AT1G70830 32.237 152 100 3 3 152 22 172 7.35e-22 88.6
MsG0880043937.01.T01 AT1G70830 32.237 152 100 3 3 152 22 172 2.14e-21 88.6
MsG0880043937.01.T01 AT1G70830 30.921 152 102 3 3 152 184 334 7.16e-20 84.7
MsG0880043937.01.T01 AT1G70890 30.921 152 102 3 3 152 7 157 2.69e-21 84.7
MsG0880043937.01.T01 AT1G70830 30.921 152 102 3 3 152 137 287 3.19e-20 84.7
MsG0880043937.01.T01 AT1G70850 30.667 150 101 3 3 150 9 157 7.76e-20 82.8
MsG0880043937.01.T01 AT1G70850 30.667 150 101 3 3 150 9 157 1.81e-19 83.2
MsG0880043937.01.T01 AT1G70850 32.394 142 93 3 13 152 175 315 1.10e-18 81.3
MsG0880043937.01.T01 AT1G70850 30.667 150 101 3 3 150 9 157 1.81e-19 83.2
MsG0880043937.01.T01 AT1G70850 32.394 142 93 3 13 152 175 315 1.10e-18 81.3
MsG0880043937.01.T01 AT1G23120 28.105 153 105 3 1 153 1 148 9.29e-19 78.2
MsG0880043937.01.T01 AT1G23120 28.571 154 103 4 2 153 50 198 4.56e-18 77.4
MsG0880043937.01.T01 AT1G23130 27.815 151 107 2 3 152 10 159 8.74e-18 75.9
MsG0880043937.01.T01 AT1G70870 32.450 151 89 4 1 151 1 138 4.10e-17 73.6
MsG0880043937.01.T01 AT4G23670 30.921 152 102 3 1 151 1 150 6.21e-17 73.6
MsG0880043937.01.T01 AT5G28000 28.947 152 105 3 3 152 13 163 7.67e-17 73.6
MsG0880043937.01.T01 AT1G70880 26.974 152 108 3 3 152 8 158 1.16e-16 72.8
MsG0880043937.01.T01 AT1G24020 29.870 154 104 3 1 152 1 152 1.30e-15 70.1
MsG0880043937.01.T01 AT1G24020 29.870 154 104 3 1 152 1 152 1.30e-15 70.1
MsG0880043937.01.T01 AT2G01520 27.632 152 107 3 1 151 1 150 2.94e-15 68.9
MsG0880043937.01.T01 AT1G14930 27.389 157 111 3 1 156 1 155 1.22e-14 67.4
MsG0880043937.01.T01 AT2G01530 28.947 152 105 3 1 151 1 150 3.36e-14 66.2
MsG0880043937.01.T01 AT1G35310 28.169 142 99 3 13 153 12 151 1.10e-13 65.1
MsG0880043937.01.T01 AT1G35260 27.815 151 99 4 7 152 6 151 1.33e-13 64.7
MsG0880043937.01.T01 AT4G14060 27.632 152 107 3 1 151 1 150 2.69e-13 63.9
MsG0880043937.01.T01 AT1G14940 27.152 151 107 3 1 150 1 149 1.71e-12 62.0
MsG0880043937.01.T01 AT1G30990 28.571 154 100 5 1 150 1 148 2.03e-12 61.6
MsG0880043937.01.T01 AT4G23680 25.000 152 111 3 1 151 1 150 2.30e-12 61.6
MsG0880043937.01.T01 AT1G14950 24.841 157 115 3 1 156 1 155 1.84e-11 59.3

Find 29 sgRNAs with CRISPR-Local

Find 77 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACTTATTTCTTATGACTTA+TGG 0.173467 8:+32977425 None:intergenic
GGTAAACTTAGCACTGAAAT+TGG 0.239596 8:-32978862 MsG0880043937.01.T01:CDS
AGGTATTCCATCCAACTATT+TGG 0.260874 8:+32977503 None:intergenic
GTGTTTGTGTCTTCAATAAA+TGG 0.262059 8:-32977267 MsG0880043937.01.T01:three_prime_UTR
GGCATATTGCATATGCATAT+TGG 0.310006 8:-32977337 MsG0880043937.01.T01:three_prime_UTR
ATGGCTCTACTGTAGTTAAA+TGG 0.325362 8:-32977565 MsG0880043937.01.T01:CDS
TTCATCACCTTGATGCAGCT+TGG 0.437287 8:+32978753 None:intergenic
GTAAACTTAGCACTGAAATT+GGG 0.446714 8:-32978861 MsG0880043937.01.T01:CDS
AGAAATTGATGAGCACTATA+AGG 0.468901 8:-32977624 MsG0880043937.01.T01:CDS
AGAATCGTCCACAAGATCTT+CGG 0.493976 8:-32977650 MsG0880043937.01.T01:CDS
TATTCCATCCAACTATTTGG+AGG 0.505271 8:+32977506 None:intergenic
AGCTGCATCAAGGTGATGAA+TGG 0.510022 8:-32978750 MsG0880043937.01.T01:CDS
TTTCTTCACCGAAGATCTTG+TGG 0.512241 8:+32977642 None:intergenic
TCATAAGAAATAAGTAGTAA+TGG 0.515556 8:-32977418 MsG0880043937.01.T01:three_prime_UTR
CATCATTGAAGTGATTGATA+AGG 0.521648 8:-32977591 MsG0880043937.01.T01:CDS
TAATGGAATGGCTTAATGCA+TGG 0.530715 8:-32977401 MsG0880043937.01.T01:three_prime_UTR
AGAAATAAGTAGTAATGGAA+TGG 0.530981 8:-32977413 MsG0880043937.01.T01:three_prime_UTR
ATGTTGAACCTCCAAATAGT+TGG 0.544310 8:-32977514 MsG0880043937.01.T01:CDS
CGATTTCACAACAGAGGGCA+AGG 0.552186 8:-32977720 MsG0880043937.01.T01:intron
AATGGAATGGCTTAATGCAT+GGG 0.552317 8:-32977400 MsG0880043937.01.T01:three_prime_UTR
CATGAAACCAAGCTGCATCA+AGG 0.562710 8:-32978760 MsG0880043937.01.T01:CDS
TGAACCTCCAAATAGTTGGA+TGG 0.602941 8:-32977510 MsG0880043937.01.T01:CDS
TGTCTCGAGTGCATTTGCTA+AGG 0.617854 8:+32977483 None:intergenic
ACTCTGATTCAGTTAAACAC+TGG 0.620261 8:-32978723 MsG0880043937.01.T01:CDS
TAGTTTGAAGAACTTGTCAG+AGG 0.640528 8:+32978828 None:intergenic
GAAGTGATTGATAAGGCTGA+TGG 0.645628 8:-32977584 MsG0880043937.01.T01:CDS
TCATCTTGTCAAGCAAGCAA+AGG 0.651550 8:-32977453 MsG0880043937.01.T01:CDS
ATGGAATGGCTTAATGCATG+GGG 0.652110 8:-32977399 MsG0880043937.01.T01:three_prime_UTR
AAACACTGGACTCATGTCCG+AGG 0.721608 8:-32978709 MsG0880043937.01.T01:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAACATATTGTAAAATTT+GGG - Chr8:32977659-32977678 MsG0880043937.01.T01:CDS 10.0%
!! AATTCCAATAAAAATTAAAA+CGG + Chr8:32978380-32978399 None:intergenic 10.0%
!! ATCATCATTATATATAAAAT+TGG - Chr8:32978139-32978158 MsG0880043937.01.T01:intron 10.0%
!! ACCATTAAATTAAAATTAGA+TGG - Chr8:32977929-32977948 MsG0880043937.01.T01:intron 15.0%
!! AGTATAATTATAGAAACATA+TGG + Chr8:32978313-32978332 None:intergenic 15.0%
!! ATTAGAAATATAATTTACCT+CGG + Chr8:32977440-32977459 None:intergenic 15.0%
!! CAATAACATATTGTAAAATT+TGG - Chr8:32977658-32977677 MsG0880043937.01.T01:CDS 15.0%
!!! AGATGAATATTTTAATTGAA+CGG - Chr8:32978022-32978041 MsG0880043937.01.T01:intron 15.0%
!!! ATAATTTAAAACTATTTGGT+TGG + Chr8:32978211-32978230 None:intergenic 15.0%
!!! TGTTTACAATTTGTTTTTTT+GGG - Chr8:32978253-32978272 MsG0880043937.01.T01:intron 15.0%
!!! TTAACCGTTTTAATTTTTAT+TGG - Chr8:32978373-32978392 MsG0880043937.01.T01:intron 15.0%
!!! TTAGTTTTTCTTTTCAAAAA+TGG - Chr8:32977236-32977255 MsG0880043937.01.T01:three_prime_UTR 15.0%
!!! TTGTTTACAATTTGTTTTTT+TGG - Chr8:32978252-32978271 MsG0880043937.01.T01:intron 15.0%
!! AACCAATCAACAATTAATAA+TGG - Chr8:32977849-32977868 MsG0880043937.01.T01:intron 20.0%
!! AAGAAAAAGAAAGATAGAAA+GGG + Chr8:32977213-32977232 None:intergenic 20.0%
!! ATTCAAGATCAATATCAATA+TGG + Chr8:32977791-32977810 None:intergenic 20.0%
!! GATCTAAGTAAAAGATATAA+AGG - Chr8:32977746-32977765 MsG0880043937.01.T01:intron 20.0%
!! TAAAAATATGACAGATCTAA+AGG - Chr8:32977710-32977729 MsG0880043937.01.T01:intron 20.0%
!! TAAGAAAAAGAAAGATAGAA+AGG + Chr8:32977214-32977233 None:intergenic 20.0%
!! TCATAAGAAATAAGTAGTAA+TGG - Chr8:32978711-32978730 MsG0880043937.01.T01:CDS 20.0%
!! TTCCATTATTAATTGTTGAT+TGG + Chr8:32977854-32977873 None:intergenic 20.0%
!!! GACATCTTTAAATTTCATTT+TGG - Chr8:32977589-32977608 MsG0880043937.01.T01:CDS 20.0%
!!! GCCATCTAATTTTAATTTAA+TGG + Chr8:32977933-32977952 None:intergenic 20.0%
!!! GTTTACAATTTGTTTTTTTG+GGG - Chr8:32978254-32978273 MsG0880043937.01.T01:intron 20.0%
!!! TACTTATTTCTTATGACTTA+TGG + Chr8:32978707-32978726 None:intergenic 20.0%
!!! TATAAAATTGGTTGATTTGA+TGG - Chr8:32978151-32978170 MsG0880043937.01.T01:intron 20.0%
!!! TTTAGATCTGTCATATTTTT+AGG + Chr8:32977711-32977730 None:intergenic 20.0%
!!! TTTTTATGGAATAAATGTGT+CGG - Chr8:32978832-32978851 MsG0880043937.01.T01:CDS 20.0%
! ATGTACAAGTACAACTATAA+AGG - Chr8:32978051-32978070 MsG0880043937.01.T01:intron 25.0%
! ATTTCTAATAACACTAACAC+TGG - Chr8:32977451-32977470 MsG0880043937.01.T01:CDS 25.0%
! GATATATATAGCAAAAGTGA+TGG - Chr8:32977877-32977896 MsG0880043937.01.T01:intron 25.0%
!! AGAAATAAGTAGTAATGGAA+TGG - Chr8:32978716-32978735 MsG0880043937.01.T01:CDS 25.0%
!!! AAATGGTTTTTTGTGCTTTT+TGG - Chr8:32978879-32978898 MsG0880043937.01.T01:exon 25.0%
!!! AGTTTGTGTTATTTTCGTAA+TGG - Chr8:32978771-32978790 MsG0880043937.01.T01:CDS 25.0%
!!! GTTTTCATCTTTCGTTTTTA+TGG - Chr8:32978818-32978837 MsG0880043937.01.T01:CDS 25.0%
!!! TTGTCTTCTGATTTTTAACT+TGG - Chr8:32977516-32977535 MsG0880043937.01.T01:CDS 25.0%
AGAAATTGATGAGCACTATA+AGG - Chr8:32978505-32978524 MsG0880043937.01.T01:intron 30.0%
CATCATTGAAGTGATTGATA+AGG - Chr8:32978538-32978557 MsG0880043937.01.T01:intron 30.0%
GTGTTTGTGTCTTCAATAAA+TGG - Chr8:32978862-32978881 MsG0880043937.01.T01:CDS 30.0%
! GTAAACTTAGCACTGAAATT+GGG - Chr8:32977268-32977287 MsG0880043937.01.T01:three_prime_UTR 30.0%
! TTGATTTGATGGTTGGAATA+GGG - Chr8:32978162-32978181 MsG0880043937.01.T01:intron 30.0%
!! AAACTATTTGGTTGGCTTTA+AGG + Chr8:32978203-32978222 None:intergenic 30.0%
!! AAATTGGTTGATTTGATGGT+TGG - Chr8:32978155-32978174 MsG0880043937.01.T01:intron 30.0%
!! ACTGGATTTATGTGCTATTT+CGG - Chr8:32977469-32977488 MsG0880043937.01.T01:CDS 30.0%
!! ATAGCAAAAGTGATGGATTT+AGG - Chr8:32977884-32977903 MsG0880043937.01.T01:intron 30.0%
!! GCTTTAAGGATCTCTAATTT+AGG + Chr8:32978189-32978208 None:intergenic 30.0%
AATGGAATGGCTTAATGCAT+GGG - Chr8:32978729-32978748 MsG0880043937.01.T01:CDS 35.0%
ACTCTGATTCAGTTAAACAC+TGG - Chr8:32977406-32977425 MsG0880043937.01.T01:three_prime_UTR 35.0%
AGGTATTCCATCCAACTATT+TGG + Chr8:32978629-32978648 None:intergenic 35.0%
ATGTTGAACCTCCAAATAGT+TGG - Chr8:32978615-32978634 MsG0880043937.01.T01:intron 35.0%
GGCATATTGCATATGCATAT+TGG - Chr8:32978792-32978811 MsG0880043937.01.T01:CDS 35.0%
TAATGGAATGGCTTAATGCA+TGG - Chr8:32978728-32978747 MsG0880043937.01.T01:CDS 35.0%
TAGTTTGAAGAACTTGTCAG+AGG + Chr8:32977304-32977323 None:intergenic 35.0%
TATTCCATCCAACTATTTGG+AGG + Chr8:32978626-32978645 None:intergenic 35.0%
! ATGGCTCTACTGTAGTTAAA+TGG - Chr8:32978564-32978583 MsG0880043937.01.T01:intron 35.0%
! GGTAAACTTAGCACTGAAAT+TGG - Chr8:32977267-32977286 MsG0880043937.01.T01:three_prime_UTR 35.0%
! TGATTTGATGGTTGGAATAG+GGG - Chr8:32978163-32978182 MsG0880043937.01.T01:intron 35.0%
! TTTTCAAAAATGGTGCTTGC+TGG - Chr8:32977246-32977265 MsG0880043937.01.T01:three_prime_UTR 35.0%
!! GTTGATTTGATGGTTGGAAT+AGG - Chr8:32978161-32978180 MsG0880043937.01.T01:intron 35.0%
ACCTTCGATTTCACAACAGA+GGG - Chr8:32978404-32978423 MsG0880043937.01.T01:intron 40.0%
AGAATCGTCCACAAGATCTT+CGG - Chr8:32978479-32978498 MsG0880043937.01.T01:intron 40.0%
ATGGAATGGCTTAATGCATG+GGG - Chr8:32978730-32978749 MsG0880043937.01.T01:CDS 40.0%
GAAGTGATTGATAAGGCTGA+TGG - Chr8:32978545-32978564 MsG0880043937.01.T01:intron 40.0%
TACCTTCGATTTCACAACAG+AGG - Chr8:32978403-32978422 MsG0880043937.01.T01:intron 40.0%
TCATCTTGTCAAGCAAGCAA+AGG - Chr8:32978676-32978695 MsG0880043937.01.T01:intron 40.0%
TGAACCTCCAAATAGTTGGA+TGG - Chr8:32978619-32978638 MsG0880043937.01.T01:intron 40.0%
TTTCTTCACCGAAGATCTTG+TGG + Chr8:32978490-32978509 None:intergenic 40.0%
AGCTGCATCAAGGTGATGAA+TGG - Chr8:32977379-32977398 MsG0880043937.01.T01:three_prime_UTR 45.0%
CATGAAACCAAGCTGCATCA+AGG - Chr8:32977369-32977388 MsG0880043937.01.T01:three_prime_UTR 45.0%
TTCATCACCTTGATGCAGCT+TGG + Chr8:32977379-32977398 None:intergenic 45.0%
! TGTCTCGAGTGCATTTGCTA+AGG + Chr8:32978649-32978668 None:intergenic 45.0%
!!! AATAATAATTTAAAACTATT+TGG + Chr8:32978215-32978234 None:intergenic 5.0%
!!! ATTTTATTTAAGAAAAAATT+GGG - Chr8:32978285-32978304 MsG0880043937.01.T01:intron 5.0%
!!! TATTTTATTTAAGAAAAAAT+TGG - Chr8:32978284-32978303 MsG0880043937.01.T01:intron 5.0%
AAACACTGGACTCATGTCCG+AGG - Chr8:32977420-32977439 MsG0880043937.01.T01:three_prime_UTR 50.0%
CGATTTCACAACAGAGGGCA+AGG - Chr8:32978409-32978428 MsG0880043937.01.T01:intron 50.0%
GCCCTCTGTTGTGAAATCGA+AGG + Chr8:32978408-32978427 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
Chr8 gene 32977203 32978948 32977203 ID=MsG0880043937.01;Name=MsG0880043937.01
Chr8 mRNA 32977203 32978948 32977203 ID=MsG0880043937.01.T01;Parent=MsG0880043937.01;Name=MsG0880043937.01.T01;_AED=0.30;_eAED=0.30;_QI=52|1|1|1|1|1|2|239|157
Chr8 exon 32977203 32977728 32977203 ID=MsG0880043937.01.T01:exon:8111;Parent=MsG0880043937.01.T01
Chr8 exon 32978710 32978948 32978710 ID=MsG0880043937.01.T01:exon:8110;Parent=MsG0880043937.01.T01
Chr8 five_prime_UTR 32978897 32978948 32978897 ID=MsG0880043937.01.T01:five_prime_utr;Parent=MsG0880043937.01.T01
Chr8 CDS 32978710 32978896 32978710 ID=MsG0880043937.01.T01:cds;Parent=MsG0880043937.01.T01
Chr8 CDS 32977442 32977728 32977442 ID=MsG0880043937.01.T01:cds;Parent=MsG0880043937.01.T01
Chr8 three_prime_UTR 32977203 32977441 32977203 ID=MsG0880043937.01.T01:three_prime_utr;Parent=MsG0880043937.01.T01
Gene Sequence

>MsG0880043937.01.T01

ATGGTGCTTGCTGGTAAACTTAGCACTGAAATTGGGATCAAAGCATCCTCTGACAAGTTCTTCAAACTATTCGCATCAAATATTCACGAACTGCAAAACATTTGTGAAAGTGTTCATGAAACCAAGCTGCATCAAGGTGATGAATGGCATCACTCTGATTCAGTTAAACACTGGACTCATGTCCGAGAGGGCAAGGTATACACATGTCATGAGAGTTTCGAAGAAATTGATGAAAAGAACAAAAGAATCGTCCACAAGATCTTCGGTGAAGAAATTGATGAGCACTATAAGGTCTTTAAGCTCATCATTGAAGTGATTGATAAGGCTGATGGCTCTACTGTAGTTAAATGGACTGTTGAATATGAGAAGATCAATGAAGATGTTGAACCTCCAAATAGTTGGATGGAATACCTTAGCAAATGCACTCGAGACATTGATGCTCATCTTGTCAAGCAAGCAAAGGTTGCTCCATAA

Protein sequence

>MsG0880043937.01.T01

MVLAGKLSTEIGIKASSDKFFKLFASNIHELQNICESVHETKLHQGDEWHHSDSVKHWTHVREGKVYTCHESFEEIDEKNKRIVHKIFGEEIDEHYKVFKLIIEVIDKADGSTVVKWTVEYEKINEDVEPPNSWMEYLSKCTRDIDAHLVKQAKVAP*