AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780037054.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780037054.01.T01 MTR_7g026320 100.000 227 0 0 1 227 1 227 1.65e-171 471
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780037054.01.T01 AT3G55420 65.534 206 60 3 26 227 18 216 4.40e-85 252

Find 34 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTGAGCTTAAAGATTTCT+TGG 0.245364 7:-18640122 MsG0780037054.01.T01:CDS
GTGTATGCTGTTAGAGAAAT+TGG 0.256026 7:-18640196 MsG0780037054.01.T01:CDS
AAACCAACCTCAGAAGTTAA+AGG 0.274880 7:-18640604 MsG0780037054.01.T01:CDS
AAATGGCTAGGTTCAAATTC+AGG 0.286811 7:-18640240 MsG0780037054.01.T01:CDS
AATGTTCTGTTGATAGAATT+TGG 0.315336 7:-18640324 MsG0780037054.01.T01:CDS
TAATTAATACTTCTTTGATT+TGG 0.320121 7:+18640076 None:intergenic
GTTAGAGAAATTGGAAGCTT+TGG 0.336072 7:-18640187 MsG0780037054.01.T01:CDS
GAAGTTAAAGGTATAAGATT+TGG 0.355820 7:-18640592 MsG0780037054.01.T01:CDS
TTACTCTCAATCTCAAAATC+AGG 0.362211 7:-18640056 MsG0780037054.01.T01:CDS
AACTTCTGAGGTTGGTTTGA+AGG 0.367774 7:+18640609 None:intergenic
TGTGAACAAGAAACTCATAA+AGG 0.455332 7:-18640278 MsG0780037054.01.T01:CDS
GCTAGGATTGTGAAATTTGT+TGG 0.455562 7:+18640433 None:intergenic
GTGAACAAGAAACTCATAAA+GGG 0.463123 7:-18640277 MsG0780037054.01.T01:CDS
AAACTCATAAAGGGTCTTTG+TGG 0.468332 7:-18640268 MsG0780037054.01.T01:CDS
ATACCTTTAACTTCTGAGGT+TGG 0.471100 7:+18640601 None:intergenic
ATCTCAAAATCAGGTTGCTA+TGG 0.477887 7:-18640047 MsG0780037054.01.T01:CDS
ATTTCTTTGATCCAAGCCCT+AGG 0.480022 7:+18640386 None:intergenic
GGCAAGAATGCTGTTGTTGA+AGG 0.519600 7:+18640454 None:intergenic
TCTTATACCTTTAACTTCTG+AGG 0.522452 7:+18640597 None:intergenic
GTTAAAGGTATAAGATTTGG+TGG 0.527957 7:-18640589 MsG0780037054.01.T01:CDS
TTGTTTGAGAATTAGGATGT+TGG 0.533338 7:+18640653 None:intergenic
AGAACTTTGAGAAGTTCAAG+AGG 0.536254 7:+18640526 None:intergenic
CACACACTAACCCTAGGGCT+TGG 0.546093 7:-18640397 MsG0780037054.01.T01:CDS
TGTGCCATGCTTGATGTGCT+AGG 0.551608 7:+18640416 None:intergenic
GCATGGCACACACTAACCCT+AGG 0.552987 7:-18640403 MsG0780037054.01.T01:CDS
GCTAGGTTCAAATTCAGGAA+AGG 0.582435 7:-18640235 MsG0780037054.01.T01:CDS
TTTCTTTGATCCAAGCCCTA+GGG 0.582873 7:+18640387 None:intergenic
TGGCCACATGAAATAGCACT+TGG 0.594109 7:-18640304 MsG0780037054.01.T01:CDS
AATGCTGTTGTTGAAGGGAA+AGG 0.602603 7:+18640460 None:intergenic
TGGTTTGAAGGAGAAATCTG+AGG 0.603282 7:+18640621 None:intergenic
CAATCCTAGCACATCAAGCA+TGG 0.615793 7:-18640420 MsG0780037054.01.T01:CDS
GCAAGAATGCTGTTGTTGAA+GGG 0.631568 7:+18640455 None:intergenic
CATGGCACACACTAACCCTA+GGG 0.642128 7:-18640402 MsG0780037054.01.T01:CDS
TCACCAAGTGCTATTTCATG+TGG 0.645451 7:+18640301 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATTAATACTTCTTTGATT+TGG + Chr7:18640666-18640685 None:intergenic 15.0%
!!! GAAATGATTTTACTATGAAT+TGG + Chr7:18640176-18640195 None:intergenic 20.0%
!! AATGTTCTGTTGATAGAATT+TGG - Chr7:18640415-18640434 MsG0780037054.01.T01:CDS 25.0%
!! GAAGTTAAAGGTATAAGATT+TGG - Chr7:18640147-18640166 MsG0780037054.01.T01:CDS 25.0%
!!! TGTTTTGTTGTTTGAGAATT+AGG + Chr7:18640096-18640115 None:intergenic 25.0%
GTGAACAAGAAACTCATAAA+GGG - Chr7:18640462-18640481 MsG0780037054.01.T01:CDS 30.0%
TGTGAACAAGAAACTCATAA+AGG - Chr7:18640461-18640480 MsG0780037054.01.T01:CDS 30.0%
TGTTGAGCTTAAAGATTTCT+TGG - Chr7:18640617-18640636 MsG0780037054.01.T01:CDS 30.0%
TTACTCTCAATCTCAAAATC+AGG - Chr7:18640683-18640702 MsG0780037054.01.T01:CDS 30.0%
TTGTTTGAGAATTAGGATGT+TGG + Chr7:18640089-18640108 None:intergenic 30.0%
! GTTAAAGGTATAAGATTTGG+TGG - Chr7:18640150-18640169 MsG0780037054.01.T01:CDS 30.0%
! TCTTATACCTTTAACTTCTG+AGG + Chr7:18640145-18640164 None:intergenic 30.0%
!! AGAGGTTTTGCTTTTCTTAT+TGG + Chr7:18640198-18640217 None:intergenic 30.0%
!!! GGAAGTTTTTTGTTGTCTTT+TGG + Chr7:18640261-18640280 None:intergenic 30.0%
!!! TTGTCTTTTGGTTTTGTTGT+TGG + Chr7:18640249-18640268 None:intergenic 30.0%
AAACCAACCTCAGAAGTTAA+AGG - Chr7:18640135-18640154 MsG0780037054.01.T01:CDS 35.0%
AAACTCATAAAGGGTCTTTG+TGG - Chr7:18640471-18640490 MsG0780037054.01.T01:CDS 35.0%
AAATGGCTAGGTTCAAATTC+AGG - Chr7:18640499-18640518 MsG0780037054.01.T01:CDS 35.0%
GCTAGGATTGTGAAATTTGT+TGG + Chr7:18640309-18640328 None:intergenic 35.0%
GTGTATGCTGTTAGAGAAAT+TGG - Chr7:18640543-18640562 MsG0780037054.01.T01:CDS 35.0%
GTTAGAGAAATTGGAAGCTT+TGG - Chr7:18640552-18640571 MsG0780037054.01.T01:CDS 35.0%
! AGAACTTTGAGAAGTTCAAG+AGG + Chr7:18640216-18640235 None:intergenic 35.0%
! ATCTCAAAATCAGGTTGCTA+TGG - Chr7:18640692-18640711 MsG0780037054.01.T01:CDS 35.0%
!! ATACCTTTAACTTCTGAGGT+TGG + Chr7:18640141-18640160 None:intergenic 35.0%
!!! CTTTTGGTTTTGTTGTTGGT+GGG + Chr7:18640245-18640264 None:intergenic 35.0%
!!! TCTTTTGGTTTTGTTGTTGG+TGG + Chr7:18640246-18640265 None:intergenic 35.0%
!!! TTTGTGGTTTTGAAATGGCT+AGG - Chr7:18640487-18640506 MsG0780037054.01.T01:CDS 35.0%
AATGCTGTTGTTGAAGGGAA+AGG + Chr7:18640282-18640301 None:intergenic 40.0%
ATTTCTTTGATCCAAGCCCT+AGG + Chr7:18640356-18640375 None:intergenic 40.0%
GCAAGAATGCTGTTGTTGAA+GGG + Chr7:18640287-18640306 None:intergenic 40.0%
GCTAGGTTCAAATTCAGGAA+AGG - Chr7:18640504-18640523 MsG0780037054.01.T01:CDS 40.0%
TGGTTTGAAGGAGAAATCTG+AGG + Chr7:18640121-18640140 None:intergenic 40.0%
TTTCTTTGATCCAAGCCCTA+GGG + Chr7:18640355-18640374 None:intergenic 40.0%
!! AACTTCTGAGGTTGGTTTGA+AGG + Chr7:18640133-18640152 None:intergenic 40.0%
!! TCACCAAGTGCTATTTCATG+TGG + Chr7:18640441-18640460 None:intergenic 40.0%
!!! GGGTCTTTGTGGTTTTGAAA+TGG - Chr7:18640482-18640501 MsG0780037054.01.T01:CDS 40.0%
CAATCCTAGCACATCAAGCA+TGG - Chr7:18640319-18640338 MsG0780037054.01.T01:CDS 45.0%
GGCAAGAATGCTGTTGTTGA+AGG + Chr7:18640288-18640307 None:intergenic 45.0%
TGGCCACATGAAATAGCACT+TGG - Chr7:18640435-18640454 MsG0780037054.01.T01:CDS 45.0%
CATGGCACACACTAACCCTA+GGG - Chr7:18640337-18640356 MsG0780037054.01.T01:CDS 50.0%
TGTGCCATGCTTGATGTGCT+AGG + Chr7:18640326-18640345 None:intergenic 50.0%
CACACACTAACCCTAGGGCT+TGG - Chr7:18640342-18640361 MsG0780037054.01.T01:CDS 55.0%
GCATGGCACACACTAACCCT+AGG - Chr7:18640336-18640355 MsG0780037054.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr7 gene 18640039 18640722 18640039 ID=MsG0780037054.01;Name=MsG0780037054.01
Chr7 mRNA 18640039 18640722 18640039 ID=MsG0780037054.01.T01;Parent=MsG0780037054.01;Name=MsG0780037054.01.T01;_AED=0.32;_eAED=0.32;_QI=0|-1|0|1|-1|1|1|0|227
Chr7 exon 18640039 18640722 18640039 ID=MsG0780037054.01.T01:exon:3019;Parent=MsG0780037054.01.T01
Chr7 CDS 18640039 18640722 18640039 ID=MsG0780037054.01.T01:cds;Parent=MsG0780037054.01.T01
Gene Sequence

>MsG0780037054.01.T01

ATGGGAGAAAAAGAGAAGAACAAGAACAATAACAAGAAACAAAAACACCAACATCCTAATTCTCAAACAACAAAACAAACCTCAGATTTCTCCTTCAAACCAACCTCAGAAGTTAAAGGTATAAGATTTGGTGGCCAATTCATAGTAAAATCATTTCCAATAAGAAAAGCAAAACCTCTTGAACTTCTCAAAGTTCTTTCTTTCCCACCAACAACAAAACCAAAAGACAACAAAAAACTTCCTTTCCCTTCAACAACAGCATTCTTGCCAACAAATTTCACAATCCTAGCACATCAAGCATGGCACACACTAACCCTAGGGCTTGGATCAAAGAAATCAAAAGTTCTTGTGTTTGTGTTTGAAACAGAAGCTATGAAATGTTCTGTTGATAGAATTTGGCCACATGAAATAGCACTTGGTGATGTGAACAAGAAACTCATAAAGGGTCTTTGTGGTTTTGAAATGGCTAGGTTCAAATTCAGGAAAGGTTGTTTGACTTTTTATGTGTATGCTGTTAGAGAAATTGGAAGCTTTGGTTTTCTTTGTGCTGAAGATTTGAAGACTATTCTTCAATCTGTTGTTGAGCTTAAAGATTTCTTGGATCATACTGTTATGCTTGCTATGCCAAATCAAAGAAGTATTAATTACTCTCAATCTCAAAATCAGGTTGCTATGGCTCATTAA

Protein sequence

>MsG0780037054.01.T01

MGEKEKNKNNNKKQKHQHPNSQTTKQTSDFSFKPTSEVKGIRFGGQFIVKSFPIRKAKPLELLKVLSFPPTTKPKDNKKLPFPSTTAFLPTNFTILAHQAWHTLTLGLGSKKSKVLVFVFETEAMKCSVDRIWPHEIALGDVNKKLIKGLCGFEMARFKFRKGCLTFYVYAVREIGSFGFLCAEDLKTILQSVVELKDFLDHTVMLAMPNQRSINYSQSQNQVAMAH*