AlfalfaGEDB | MsG0780040859.01

M. sativa cultivar ZhongmuNo.1 / MsG0780040859.01

KEGG: K11252
GO: GO:0000786 GO:0003677 GO:0046982
PFAM: PF00125

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780040859.01.T01	XP_003625510.1	99.275	138	1	0	1	138	1	138	1.59E-90	268

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780040859.01.T01	Q1SU99	99.275	138	1	0	1	138	1	138	1.98E-93	268

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780040859.01.T01	B7FH68	99.275	138	1	0	1	138	1	138	7.58e-91	268

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0780040844.01	MsG0780040859.01	0.811257	7.665642e-51	4.722400e-48
MsG0780040845.01	MsG0780040859.01	0.805129	1.549212e-49	8.131546e-47
MsG0780040859.01	MsG0880045145.01	0.836744	7.820567e-57	9.946953e-54
MsG0780040859.01	MsG0880047472.01	0.801444	8.979815e-49	4.289041e-46
MsG0280011003.01	MsG0780040859.01	0.807081	6.015095e-50	3.319280e-47
MsG0380014289.01	MsG0780040859.01	0.816763	4.676449e-52	3.343294e-49
MsG0680031507.01	MsG0780040859.01	0.828244	1.001247e-54	9.895754e-52
MsG0480021302.01	MsG0780040859.01	0.827582	1.445123e-54	1.400827e-51
MsG0380015444.01	MsG0780040859.01	0.819365	1.206821e-52	9.267150e-50
MsG0780037240.01	MsG0780040859.01	0.803534	3.330340e-49	1.677786e-46

PPI

Gene1	Gene2	Type
MsG0480020877.01	MsG0780040859.01	PPI
MsG0780040859.01	MsG0880042844.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780040859.01.T01	MTR_7g099960	99.275	138	1	0	1	138	1	138	1.92e-94	268
MsG0780040859.01.T01	MTR_4g063240	85.811	148	10	3	1	138	1	147	2.83e-81	236
MsG0780040859.01.T01	MTR_1g090780	93.525	139	6	3	1	138	1	137	6.50e-79	229
MsG0780040859.01.T01	MTR_1g492910	93.525	139	6	3	1	138	1	137	7.10e-79	229
MsG0780040859.01.T01	MTR_4g063220	86.577	149	8	3	1	138	1	148	1.57e-77	226
MsG0780040859.01.T01	MTR_4g063990	86.577	149	8	3	1	138	1	148	1.57e-77	226
MsG0780040859.01.T01	MTR_4g063200	86.577	149	8	3	1	138	1	148	1.57e-77	226
MsG0780040859.01.T01	MTR_4g064020	86.577	149	8	3	1	138	1	148	1.57e-77	226
MsG0780040859.01.T01	MTR_4g071180	86.577	149	8	3	1	138	1	148	1.47e-76	224
MsG0780040859.01.T01	MTR_4g070240	86.577	149	8	3	1	138	1	148	1.47e-76	224
MsG0780040859.01.T01	MTR_4g088025	87.500	144	11	3	1	138	1	143	4.70e-75	220
MsG0780040859.01.T01	MTR_4g070245	85.235	149	10	3	1	138	1	148	5.92e-75	220
MsG0780040859.01.T01	MTR_4g063275	83.893	149	12	3	1	138	1	148	2.63e-74	218
MsG0780040859.01.T01	MTR_4g064983	85.517	145	9	3	1	134	1	144	6.26e-73	218
MsG0780040859.01.T01	MTR_1g068600	95.370	108	4	1	31	138	30	136	5.83e-65	194
MsG0780040859.01.T01	MTR_2g084480	60.674	89	35	0	49	137	136	224	2.88e-37	127
MsG0780040859.01.T01	MTR_8g069990	71.910	89	22	2	9	97	3	88	1.10e-35	119
MsG0780040859.01.T01	MTR_4g063270	90.196	51	5	0	76	126	1	51	4.56e-27	95.9
MsG0780040859.01.T01	MTR_7g114580	90.244	41	4	0	98	138	11	51	6.10e-20	77.8
MsG0780040859.01.T01	MTR_4g032777	39.744	78	47	0	45	122	33	110	7.78e-13	61.6
MsG0780040859.01.T01	MTR_2g083800	66.071	56	14	3	9	62	11	63	3.82e-11	55.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780040859.01.T01	AT2G37470	89.855	138	13	1	1	137	1	138	8.77e-79	229
MsG0780040859.01.T01	AT3G53650	89.855	138	14	0	1	138	1	138	1.27e-77	226
MsG0780040859.01.T01	AT3G45980	82.781	151	12	3	1	138	1	150	9.51e-75	219
MsG0780040859.01.T01	AT1G07790	82.550	149	14	2	1	138	1	148	8.61e-74	217
MsG0780040859.01.T01	AT2G28720	82.237	152	12	3	1	138	1	151	1.94e-73	216
MsG0780040859.01.T01	AT5G59910	85.926	135	17	1	6	138	16	150	1.59e-71	211
MsG0780040859.01.T01	AT3G46030	88.372	129	13	1	12	138	17	145	6.12e-71	209
MsG0780040859.01.T01	AT5G02570	88.550	131	9	2	11	138	5	132	2.11e-67	200
MsG0780040859.01.T01	AT5G22880	81.119	143	18	2	5	138	3	145	5.89e-66	197
MsG0780040859.01.T01	AT3G09480	82.114	123	21	1	16	138	5	126	8.61e-64	191
MsG0780040859.01.T01	AT1G08170	60.000	85	34	0	51	135	151	235	3.18e-37	127

Find 34 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATCAAGAGCTGGTGAATT+TGG	0.235894	7:+83512567	None:intergenic
CTCCGCCTTTGGTTTCTTCT+CGG	0.278156	7:+83512903	None:intergenic
GCTGTTAGATTGGTTCTTCC+TGG	0.314946	7:-83512634	MsG0780040859.01.T01:CDS
GAGATCTTCTTCTCCGCCTT+TGG	0.325858	7:+83512892	None:intergenic
ATTGGTTCTTCCTGGAGAAT+TGG	0.353160	7:-83512626	MsG0780040859.01.T01:CDS
GCCTTTGGTTTCTTCTCGGC+TGG	0.357848	7:+83512907	None:intergenic
CATCTTCAAGGTTCTGAAAC+AGG	0.359264	7:-83512803	MsG0780040859.01.T01:CDS
AAACAGGTTCATCCTGATAT+TGG	0.374570	7:-83512787	MsG0780040859.01.T01:CDS
GATTCAAACTGCTGTTAGAT+TGG	0.401031	7:-83512644	MsG0780040859.01.T01:CDS
TGCTCAGGAATCGTCTCGTT+TGG	0.412474	7:-83512704	MsG0780040859.01.T01:CDS
TTGCTTGAAACACCAATATC+AGG	0.417581	7:+83512775	None:intergenic
TGGTGTTTCAAGCAAGGCTA+TGG	0.418611	7:-83512767	MsG0780040859.01.T01:CDS
TCGGCTGGAGCTTTCTCTGC+GGG	0.433183	7:+83512922	None:intergenic
TGCTGTTTCTGAAGGGACTA+AGG	0.469671	7:-83512596	MsG0780040859.01.T01:CDS
ATACAAGATATACATCTTCA+AGG	0.488494	7:-83512815	MsG0780040859.01.T01:CDS
GCTAAACATGCTGTTTCTGA+AGG	0.504101	7:-83512604	MsG0780040859.01.T01:CDS
TCTGCGGGCTTCTTCTCCGC+GGG	0.504996	7:+83512937	None:intergenic
GCATGTTTAGCCAATTCTCC+AGG	0.514866	7:+83512616	None:intergenic
TATCTTTGAGAAACTTGCTC+AGG	0.528928	7:-83512719	MsG0780040859.01.T01:CDS
ATCTCCCTTGAAGTGATCGT+CGG	0.552358	7:+83512664	None:intergenic
CTCTGCGGGCTTCTTCTCCG+CGG	0.552987	7:+83512936	None:intergenic
GGTGTTTCAAGCAAGGCTAT+GGG	0.553844	7:-83512766	MsG0780040859.01.T01:CDS
CTCGGCTGGAGCTTTCTCTG+CGG	0.557332	7:+83512921	None:intergenic
AGAAGCCGACGATCACTTCA+AGG	0.560392	7:-83512669	MsG0780040859.01.T01:CDS
GAGAAGAAGATCTCAAAAGA+AGG	0.561490	7:-83512883	MsG0780040859.01.T01:CDS
TGATATTGGTGTTTCAAGCA+AGG	0.568075	7:-83512773	MsG0780040859.01.T01:CDS
AGGAATCGTCTCGTTTGGCT+AGG	0.569875	7:-83512699	MsG0780040859.01.T01:CDS
AAAGCAACAAAATCAAGAGC+TGG	0.570887	7:+83512558	None:intergenic
CTAAACATGCTGTTTCTGAA+GGG	0.585084	7:-83512603	MsG0780040859.01.T01:CDS
ATCAAGAATGGCTCCCACAA+AGG	0.630896	7:-83512971	None:intergenic
TCCAGCCGAGAAGAAACCAA+AGG	0.637685	7:-83512908	MsG0780040859.01.T01:CDS
AAAGGCAGAAAAGAAACCCG+CGG	0.639354	7:-83512953	MsG0780040859.01.T01:CDS
GAAGCCGACGATCACTTCAA+GGG	0.644853	7:-83512668	MsG0780040859.01.T01:CDS
AGCCGAGAAGAAACCAAAGG+CGG	0.671315	7:-83512905	MsG0780040859.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ATACAAGATATACATCTTCA+AGG	-	Chr7:83512719-83512738	MsG0780040859.01.T01:CDS	25.0%
	AAACAGGTTCATCCTGATAT+TGG	-	Chr7:83512747-83512766	MsG0780040859.01.T01:CDS	35.0%
	GAGAAGAAGATCTCAAAAGA+AGG	-	Chr7:83512651-83512670	MsG0780040859.01.T01:CDS	35.0%
	GATTCAAACTGCTGTTAGAT+TGG	-	Chr7:83512890-83512909	MsG0780040859.01.T01:CDS	35.0%
	TTGCTTGAAACACCAATATC+AGG	+	Chr7:83512762-83512781	None:intergenic	35.0%
!	TATCTTTGAGAAACTTGCTC+AGG	-	Chr7:83512815-83512834	MsG0780040859.01.T01:CDS	35.0%
!	TGATATTGGTGTTTCAAGCA+AGG	-	Chr7:83512761-83512780	MsG0780040859.01.T01:CDS	35.0%
!!	CTAAACATGCTGTTTCTGAA+GGG	-	Chr7:83512931-83512950	MsG0780040859.01.T01:CDS	35.0%
!	ATTGGTTCTTCCTGGAGAAT+TGG	-	Chr7:83512908-83512927	MsG0780040859.01.T01:CDS	40.0%
!	GGTTTCTTTTCTGCCTTTGT+GGG	+	Chr7:83512579-83512598	None:intergenic	40.0%
!!	CATCTTCAAGGTTCTGAAAC+AGG	-	Chr7:83512731-83512750	MsG0780040859.01.T01:CDS	40.0%
!!	GCTAAACATGCTGTTTCTGA+AGG	-	Chr7:83512930-83512949	MsG0780040859.01.T01:CDS	40.0%
	AAAGGCAGAAAAGAAACCCG+CGG	-	Chr7:83512581-83512600	MsG0780040859.01.T01:CDS	45.0%
	ATCTCCCTTGAAGTGATCGT+CGG	+	Chr7:83512873-83512892	None:intergenic	45.0%
	GCATGTTTAGCCAATTCTCC+AGG	+	Chr7:83512921-83512940	None:intergenic	45.0%
	GGTGTTTCAAGCAAGGCTAT+GGG	-	Chr7:83512768-83512787	MsG0780040859.01.T01:CDS	45.0%
	TGGTGTTTCAAGCAAGGCTA+TGG	-	Chr7:83512767-83512786	MsG0780040859.01.T01:CDS	45.0%
!	GCTGTTAGATTGGTTCTTCC+TGG	-	Chr7:83512900-83512919	MsG0780040859.01.T01:CDS	45.0%
!	GGGTTTCTTTTCTGCCTTTG+TGG	+	Chr7:83512580-83512599	None:intergenic	45.0%
!!	TGCTGTTTCTGAAGGGACTA+AGG	-	Chr7:83512938-83512957	MsG0780040859.01.T01:CDS	45.0%
	AGAAGCCGACGATCACTTCA+AGG	-	Chr7:83512865-83512884	MsG0780040859.01.T01:CDS	50.0%
	AGCCGAGAAGAAACCAAAGG+CGG	-	Chr7:83512629-83512648	MsG0780040859.01.T01:CDS	50.0%
	AGGAATCGTCTCGTTTGGCT+AGG	-	Chr7:83512835-83512854	MsG0780040859.01.T01:CDS	50.0%
	GAAGCCGACGATCACTTCAA+GGG	-	Chr7:83512866-83512885	MsG0780040859.01.T01:CDS	50.0%
	GAGATCTTCTTCTCCGCCTT+TGG	+	Chr7:83512645-83512664	None:intergenic	50.0%
	TCCAGCCGAGAAGAAACCAA+AGG	-	Chr7:83512626-83512645	MsG0780040859.01.T01:CDS	50.0%
	TGCTCAGGAATCGTCTCGTT+TGG	-	Chr7:83512830-83512849	MsG0780040859.01.T01:CDS	50.0%
!	CTCCGCCTTTGGTTTCTTCT+CGG	+	Chr7:83512634-83512653	None:intergenic	50.0%
!	GCCTTTGGTTTCTTCTCGGC+TGG	+	Chr7:83512630-83512649	None:intergenic	55.0%
	CTCGGCTGGAGCTTTCTCTG+CGG	+	Chr7:83512616-83512635	None:intergenic	60.0%
	TCGGCTGGAGCTTTCTCTGC+GGG	+	Chr7:83512615-83512634	None:intergenic	60.0%
	CTCTGCGGGCTTCTTCTCCG+CGG	+	Chr7:83512601-83512620	None:intergenic	65.0%
	TCTGCGGGCTTCTTCTCCGC+GGG	+	Chr7:83512600-83512619	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
Chr7	gene	83512570	83512986	83512570	ID=MsG0780040859.01;Name=MsG0780040859.01
Chr7	mRNA	83512570	83512986	83512570	ID=MsG0780040859.01.T01;Parent=MsG0780040859.01;Name=MsG0780040859.01.T01;_AED=0.23;_eAED=0.23;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|138
Chr7	exon	83512570	83512986	83512570	ID=MsG0780040859.01.T01:exon:19790;Parent=MsG0780040859.01.T01
Chr7	CDS	83512570	83512986	83512570	ID=MsG0780040859.01.T01:cds;Parent=MsG0780040859.01.T01

Gene Sequence

>MsG0780040859.01.T01

ATGGCTCCCACAAAGGCAGAAAAGAAACCCGCGGAGAAGAAGCCCGCAGAGAAAGCTCCAGCCGAGAAGAAACCAAAGGCGGAGAAGAAGATCTCAAAAGAAGGAAGCAGCGACAAGAAGAAAAAGAGAACGAAGAAAAGCGTTGAAACATACAAGATATACATCTTCAAGGTTCTGAAACAGGTTCATCCTGATATTGGTGTTTCAAGCAAGGCTATGGGAATTATGAACAGTTTCATTAACGATATCTTTGAGAAACTTGCTCAGGAATCGTCTCGTTTGGCTAGGTATAACAAGAAGCCGACGATCACTTCAAGGGAGATTCAAACTGCTGTTAGATTGGTTCTTCCTGGAGAATTGGCTAAACATGCTGTTTCTGAAGGGACTAAGGCTGTTACCAAATTCACCAGCTCTTGA

Protein sequence

>MsG0780040859.01.T01

MAPTKAEKKPAEKKPAEKAPAEKKPKAEKKISKEGSSDKKKKRTKKSVETYKIYIFKVLKQVHPDIGVSSKAMGIMNSFINDIFEKLAQESSRLARYNKKPTITSREIQTAVRLVLPGELAKHAVSEGTKAVTKFTSS*