AlfalfaGEDB | MsG0480021302.01

M. sativa cultivar ZhongmuNo.1 / MsG0480021302.01

KEGG: K11252
GO: GO:0000786 GO:0003677 GO:0046982
PFAM: PF00125

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021302.01.T01	XP_003606627.1	99.324	148	1	0	1	148	1	148	3.31E-98	289

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021302.01.T01	Q1S9I9	99.324	148	1	0	1	148	1	148	8.77E-101	288

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021302.01.T01	G7JTQ7	99.324	148	1	0	1	148	1	148	1.58e-98	289

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180002936.01	MsG0480021302.01	0.832367	9.843954e-56	1.097953e-52
MsG0180004994.01	MsG0480021302.01	0.820043	8.449810e-53	6.613629e-50
MsG0180004995.01	MsG0480021302.01	0.817515	3.168781e-52	2.312610e-49
MsG0180005048.01	MsG0480021302.01	0.845049	5.173394e-59	8.515426e-56
MsG0180006133.01	MsG0480021302.01	0.804157	2.472108e-49	1.265456e-46
MsG0380014684.01	MsG0480021302.01	0.869246	3.575691e-66	1.332551e-62
MsG0480020918.01	MsG0480021302.01	0.858283	9.159848e-63	2.325703e-59
MsG0480020920.01	MsG0480021302.01	0.851167	1.058285e-60	2.123202e-57
MsG0480020927.01	MsG0480021302.01	0.898888	3.409723e-77	4.112013e-73
MsG0480020969.01	MsG0480021302.01	0.866982	1.913336e-65	6.573238e-62
MsG0480020971.01	MsG0480021302.01	0.870829	1.085653e-66	4.283160e-63
MsG0480021015.01	MsG0480021302.01	0.885916	5.380883e-72	3.762056e-68
MsG0480021252.01	MsG0480021302.01	0.830963	2.183732e-55	2.336587e-52
MsG0480021301.01	MsG0480021302.01	0.837504	5.000479e-57	6.509139e-54
MsG0480021302.01	MsG0580025203.01	0.828907	6.926178e-55	6.978184e-52
MsG0480021302.01	MsG0580025506.01	0.819079	1.402259e-52	1.068348e-49
MsG0480021302.01	MsG0780040620.01	0.800306	1.533537e-48	7.116910e-46
MsG0480021302.01	MsG0780040844.01	0.853526	2.253904e-61	4.884619e-58
MsG0480021302.01	MsG0780040859.01	0.827582	1.445123e-54	1.400827e-51
MsG0480021302.01	MsG0780041273.01	0.810981	8.797107e-51	5.380827e-48
MsG0480021302.01	MsG0780041546.01	0.818440	1.957618e-52	1.465589e-49
MsG0480021302.01	MsG0780041592.01	0.838322	3.080273e-57	4.110753e-54
MsG0480021302.01	MsG0880045015.01	0.854000	1.646941e-61	3.625692e-58
MsG0380015234.01	MsG0480021302.01	0.841874	3.646579e-58	5.431535e-55
MsG0280010346.01	MsG0480021302.01	0.850573	1.556078e-60	3.061474e-57

PPI

Gene1	Gene2	Type
MsG0580027231.01	MsG0480021302.01	PPI
MsG0480021302.01	MsG0480023842.01	PPI
MsG0180000243.01	MsG0480021302.01	PPI
MsG0880047260.01	MsG0480021302.01	PPI
MsG0880045015.01	MsG0480021302.01	PPI
MsG0880042844.01	MsG0480021302.01	PPI
MsG0480021302.01	MsG0780041593.01	PPI
MsG0480021302.01	MsG0780036534.01	PPI
MsG0380012788.01	MsG0480021302.01	PPI
MsG0780038642.01	MsG0480021302.01	PPI
MsG0480021302.01	MsG0280007919.01	PPI
MsG0580029194.01	MsG0480021302.01	PPI
MsG0580029044.01	MsG0480021302.01	PPI
MsG0580025506.01	MsG0480021302.01	PPI
MsG0480020877.01	MsG0480021302.01	PPI
MsG0480021302.01	MsG0480022307.01	PPI
MsG0880044910.01	MsG0480021302.01	PPI
MsG0480021302.01	MsG0580025504.01	PPI
MsG0880044910.01	MsG0480021302.01	PPI
MsG0480021302.01	MsG0780041592.01	PPI
MsG0480021302.01	MsG0280008380.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021302.01.T01	MTR_4g063220	99.324	148	1	0	1	148	1	148	4.00e-102	289
MsG0480021302.01.T01	MTR_4g063990	99.324	148	1	0	1	148	1	148	4.00e-102	289
MsG0480021302.01.T01	MTR_4g063200	99.324	148	1	0	1	148	1	148	4.00e-102	289
MsG0480021302.01.T01	MTR_4g064020	99.324	148	1	0	1	148	1	148	4.00e-102	289
MsG0480021302.01.T01	MTR_4g071180	99.324	148	1	0	1	148	1	148	8.54e-102	288
MsG0480021302.01.T01	MTR_4g070240	99.324	148	1	0	1	148	1	148	8.54e-102	288
MsG0480021302.01.T01	MTR_4g070245	97.973	148	3	0	1	148	1	148	4.20e-100	284
MsG0480021302.01.T01	MTR_4g063240	98.649	148	1	1	1	148	1	147	1.15e-99	283
MsG0480021302.01.T01	MTR_4g063275	96.622	148	5	0	1	148	1	148	8.49e-99	280
MsG0480021302.01.T01	MTR_4g064983	98.611	144	2	0	1	144	1	144	8.23e-97	279
MsG0480021302.01.T01	MTR_4g088025	93.919	148	4	1	1	148	1	143	8.30e-89	255
MsG0480021302.01.T01	MTR_1g090780	88.514	148	6	3	1	148	1	137	1.68e-78	229
MsG0480021302.01.T01	MTR_1g492910	87.838	148	7	3	1	148	1	137	8.34e-78	227
MsG0480021302.01.T01	MTR_1g068600	92.793	111	4	1	38	148	30	136	3.40e-64	192
MsG0480021302.01.T01	MTR_7g099960	92.793	111	5	1	38	148	31	138	6.27e-64	192
MsG0480021302.01.T01	MTR_2g084480	61.798	89	34	0	59	147	136	224	4.83e-37	127
MsG0480021302.01.T01	MTR_8g069990	68.817	93	21	3	15	107	4	88	3.55e-34	115
MsG0480021302.01.T01	MTR_4g063270	90.196	51	5	0	86	136	1	51	5.24e-27	95.9
MsG0480021302.01.T01	MTR_7g114580	90.244	41	4	0	108	148	11	51	9.46e-20	77.4
MsG0480021302.01.T01	MTR_4g032777	36.792	106	63	2	31	132	5	110	1.73e-13	63.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021302.01.T01	AT3G45980	88.742	151	13	2	1	148	1	150	1.47e-83	242
MsG0480021302.01.T01	AT2G28720	86.093	151	18	1	1	148	1	151	9.69e-81	235
MsG0480021302.01.T01	AT1G07790	86.928	153	10	3	1	148	1	148	7.69e-80	233
MsG0480021302.01.T01	AT5G59910	91.176	136	9	2	13	148	18	150	2.65e-77	226
MsG0480021302.01.T01	AT3G46030	94.444	126	6	1	23	148	21	145	8.66e-76	222
MsG0480021302.01.T01	AT2G37470	82.432	148	15	3	1	147	1	138	6.01e-71	210
MsG0480021302.01.T01	AT3G53650	81.208	149	16	3	1	148	1	138	4.61e-69	205
MsG0480021302.01.T01	AT5G22880	87.586	145	16	2	4	148	3	145	4.25e-67	200
MsG0480021302.01.T01	AT5G02570	79.730	148	14	1	1	148	1	132	9.71e-67	199
MsG0480021302.01.T01	AT3G09480	84.127	126	16	1	23	148	5	126	2.13e-62	187
MsG0480021302.01.T01	AT1G08170	61.176	85	33	0	61	145	151	235	2.97e-37	127

Find 42 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCTGGCTTCTTCTCTCCTTT+TGG	0.266976	4:-56483081	None:intergenic
GGTAGCTTCTTTCCAGCCTT+TGG	0.299167	4:-56483168	None:intergenic
GCTTGTTCTGCCCGGTGAAT+TGG	0.304089	4:+56483443	MsG0480021302.01.T01:CDS
TTGCTTGAGATACCAATGTC+TGG	0.335174	4:-56483294	None:intergenic
CTTGTTGTACCTAGCGAGTC+TGG	0.384866	4:-56483379	None:intergenic
TCTGCGGGTTTCTTCTCTGC+TGG	0.399254	4:-56483099	None:intergenic
GCCTTTGGCTTCTTCTCCGC+CGG	0.402654	4:-56483153	None:intergenic
GCGGCTGAACCACCTTCCTT+TGG	0.410543	4:-56483189	None:intergenic
GTGGACTTCTTCTCCTCTGC+GGG	0.437960	4:-56483114	None:intergenic
TCTCTTCTTCTTCTTGTCTC+CGG	0.444808	4:-56483211	None:intergenic
TGCTGTTTCTGAGGGTACTA+AGG	0.448307	4:+56483473	MsG0480021302.01.T01:CDS
ACCCTCAGAAACAGCATGCT+TGG	0.464482	4:-56483466	None:intergenic
AGCATGCTTGGCCAATTCAC+CGG	0.471051	4:-56483454	None:intergenic
GGGAAATTCAGACCGCGGTC+AGG	0.473209	4:+56483421	MsG0480021302.01.T01:CDS
GCGGAGAAGAAGCCAAAGGC+TGG	0.504484	4:+56483156	MsG0480021302.01.T01:CDS
AAGGAAGGTGGTTCAGCCGC+CGG	0.504946	4:+56483192	MsG0480021302.01.T01:CDS
CGCCGGAGCTTTCTCTGCGA+CGG	0.521178	4:-56483136	None:intergenic
GCATGCTTGGCCAATTCACC+GGG	0.525255	4:-56483453	None:intergenic
TGCTTGAGATACCAATGTCT+GGG	0.532971	4:-56483293	None:intergenic
TGGTATCTCAAGCAAAGCCA+TGG	0.533917	4:+56483302	MsG0480021302.01.T01:CDS
GCCAAGCATGCTGTTTCTGA+GGG	0.538001	4:+56483465	MsG0480021302.01.T01:CDS
GGCCAAGCATGCTGTTTCTG+AGG	0.538906	4:+56483464	MsG0480021302.01.T01:CDS
AGAAGCCGACGATCACTTCA+AGG	0.553571	4:+56483400	MsG0480021302.01.T01:CDS
GCGGTCAGGCTTGTTCTGCC+CGG	0.555879	4:+56483435	MsG0480021302.01.T01:CDS
ATTTCCCTTGAAGTGATCGT+CGG	0.564140	4:-56483405	None:intergenic
CTTCTTCTTCTTGTCTCCGG+CGG	0.574610	4:-56483208	None:intergenic
CACCGTCGCAGAGAAAGCTC+CGG	0.578048	4:+56483134	MsG0480021302.01.T01:CDS
AAGAATCTTCCAGACTCGCT+AGG	0.594905	4:+56483370	MsG0480021302.01.T01:CDS
CGGAGCTTTCTCTGCGACGG+TGG	0.603516	4:-56483133	None:intergenic
AAACAAGTTCACCCAGACAT+TGG	0.612279	4:+56483282	MsG0480021302.01.T01:CDS
GGAAAGAAGCTACCAAAGGA+AGG	0.624050	4:+56483177	MsG0480021302.01.T01:CDS
GAAACTGTTCATGATTCCCA+TGG	0.633384	4:-56483319	None:intergenic
GGCTGGAAAGAAGCTACCAA+AGG	0.634160	4:+56483173	MsG0480021302.01.T01:CDS
GAAGCCGACGATCACTTCAA+GGG	0.638033	4:+56483401	MsG0480021302.01.T01:CDS
CGTCGCAGAGAAAGCTCCGG+CGG	0.643171	4:+56483137	MsG0480021302.01.T01:CDS
GGTATCTCAAGCAAAGCCAT+GGG	0.654333	4:+56483303	MsG0480021302.01.T01:CDS
AAGAAGCTACCAAAGGAAGG+TGG	0.666846	4:+56483180	MsG0480021302.01.T01:CDS
GGTGGACTTCTTCTCCTCTG+CGG	0.668086	4:-56483115	None:intergenic
TCCGGCGGAGAAGAAGCCAA+AGG	0.682003	4:+56483152	MsG0480021302.01.T01:CDS
TTCAAGGGAAATTCAGACCG+CGG	0.690059	4:+56483416	MsG0480021302.01.T01:CDS
GGGCAGAACAAGCCTGACCG+CGG	0.695125	4:-56483433	None:intergenic
AGCAGAGAAGAAACCCGCAG+AGG	0.698236	4:+56483101	MsG0480021302.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAACAAGTTCACCCAGACAT+TGG	+	Chr4:56483282-56483301	MsG0480021302.01.T01:CDS	40.0%
	ATTTCCCTTGAAGTGATCGT+CGG	-	Chr4:56483408-56483427	None:intergenic	40.0%
	GAAACTGTTCATGATTCCCA+TGG	-	Chr4:56483322-56483341	None:intergenic	40.0%
	TCTCTTCTTCTTCTTGTCTC+CGG	-	Chr4:56483214-56483233	None:intergenic	40.0%
	TGCTTGAGATACCAATGTCT+GGG	-	Chr4:56483296-56483315	None:intergenic	40.0%
	TTGCTTGAGATACCAATGTC+TGG	-	Chr4:56483297-56483316	None:intergenic	40.0%
	AAGAAGCTACCAAAGGAAGG+TGG	+	Chr4:56483180-56483199	MsG0480021302.01.T01:CDS	45.0%
	AAGAATCTTCCAGACTCGCT+AGG	+	Chr4:56483370-56483389	MsG0480021302.01.T01:CDS	45.0%
	GGAAAGAAGCTACCAAAGGA+AGG	+	Chr4:56483177-56483196	MsG0480021302.01.T01:CDS	45.0%
	GGTATCTCAAGCAAAGCCAT+GGG	+	Chr4:56483303-56483322	MsG0480021302.01.T01:CDS	45.0%
	TGGTATCTCAAGCAAAGCCA+TGG	+	Chr4:56483302-56483321	MsG0480021302.01.T01:CDS	45.0%
	TTCAAGGGAAATTCAGACCG+CGG	+	Chr4:56483416-56483435	MsG0480021302.01.T01:CDS	45.0%
!!	TGCTGTTTCTGAGGGTACTA+AGG	+	Chr4:56483473-56483492	MsG0480021302.01.T01:CDS	45.0%
	ACCCTCAGAAACAGCATGCT+TGG	-	Chr4:56483469-56483488	None:intergenic	50.0%
	AGAAGCCGACGATCACTTCA+AGG	+	Chr4:56483400-56483419	MsG0480021302.01.T01:CDS	50.0%
	AGCATGCTTGGCCAATTCAC+CGG	-	Chr4:56483457-56483476	None:intergenic	50.0%
	CTTCTTCTTCTTGTCTCCGG+CGG	-	Chr4:56483211-56483230	None:intergenic	50.0%
	GAAGCCGACGATCACTTCAA+GGG	+	Chr4:56483401-56483420	MsG0480021302.01.T01:CDS	50.0%
	GGCTGGAAAGAAGCTACCAA+AGG	+	Chr4:56483173-56483192	MsG0480021302.01.T01:CDS	50.0%
	GGTAGCTTCTTTCCAGCCTT+TGG	-	Chr4:56483171-56483190	None:intergenic	50.0%
!	CTTGTTGTACCTAGCGAGTC+TGG	-	Chr4:56483382-56483401	None:intergenic	50.0%
!!	GCCAAGCATGCTGTTTCTGA+GGG	+	Chr4:56483465-56483484	MsG0480021302.01.T01:CDS	50.0%
!!	GCTGGCTTCTTCTCTCCTTT+TGG	-	Chr4:56483084-56483103	None:intergenic	50.0%
	AGCAGAGAAGAAACCCGCAG+AGG	+	Chr4:56483101-56483120	MsG0480021302.01.T01:CDS	55.0%
	GCATGCTTGGCCAATTCACC+GGG	-	Chr4:56483456-56483475	None:intergenic	55.0%
	GCTTGTTCTGCCCGGTGAAT+TGG	+	Chr4:56483443-56483462	MsG0480021302.01.T01:CDS	55.0%
	GTGGACTTCTTCTCCTCTGC+GGG	-	Chr4:56483117-56483136	None:intergenic	55.0%
	TCTGCGGGTTTCTTCTCTGC+TGG	-	Chr4:56483102-56483121	None:intergenic	55.0%
!	GGTGGACTTCTTCTCCTCTG+CGG	-	Chr4:56483118-56483137	None:intergenic	55.0%
!!	GGCCAAGCATGCTGTTTCTG+AGG	+	Chr4:56483464-56483483	MsG0480021302.01.T01:CDS	55.0%
	AAGGAAGGTGGTTCAGCCGC+CGG	+	Chr4:56483192-56483211	MsG0480021302.01.T01:CDS	60.0%
	CACCGTCGCAGAGAAAGCTC+CGG	+	Chr4:56483134-56483153	MsG0480021302.01.T01:CDS	60.0%
	GCGGAGAAGAAGCCAAAGGC+TGG	+	Chr4:56483156-56483175	MsG0480021302.01.T01:CDS	60.0%
	GCGGCTGAACCACCTTCCTT+TGG	-	Chr4:56483192-56483211	None:intergenic	60.0%
	GGGAAATTCAGACCGCGGTC+AGG	+	Chr4:56483421-56483440	MsG0480021302.01.T01:CDS	60.0%
	TCCGGCGGAGAAGAAGCCAA+AGG	+	Chr4:56483152-56483171	MsG0480021302.01.T01:CDS	60.0%
!	GCCTTTGGCTTCTTCTCCGC+CGG	-	Chr4:56483156-56483175	None:intergenic	60.0%
	CGCCGGAGCTTTCTCTGCGA+CGG	-	Chr4:56483139-56483158	None:intergenic	65.0%
	CGGAGCTTTCTCTGCGACGG+TGG	-	Chr4:56483136-56483155	None:intergenic	65.0%
	CGTCGCAGAGAAAGCTCCGG+CGG	+	Chr4:56483137-56483156	MsG0480021302.01.T01:CDS	65.0%
	GGGCAGAACAAGCCTGACCG+CGG	-	Chr4:56483436-56483455	None:intergenic	65.0%
!	GCGGTCAGGCTTGTTCTGCC+CGG	+	Chr4:56483435-56483454	MsG0480021302.01.T01:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	56483075	56483521	56483075	ID=MsG0480021302.01;Name=MsG0480021302.01
Chr4	mRNA	56483075	56483521	56483075	ID=MsG0480021302.01.T01;Parent=MsG0480021302.01;Name=MsG0480021302.01.T01;_AED=0.49;_eAED=0.49;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|148
Chr4	exon	56483075	56483521	56483075	ID=MsG0480021302.01.T01:exon:19063;Parent=MsG0480021302.01.T01
Chr4	CDS	56483075	56483521	56483075	ID=MsG0480021302.01.T01:cds;Parent=MsG0480021302.01.T01

Gene Sequence

>MsG0480021302.01.T01

ATGGCGCCAAAAGGAGAGAAGAAGCCAGCAGAGAAGAAACCCGCAGAGGAGAAGAAGTCCACCGTCGCAGAGAAAGCTCCGGCGGAGAAGAAGCCAAAGGCTGGAAAGAAGCTACCAAAGGAAGGTGGTTCAGCCGCCGGAGACAAGAAGAAGAAGAGAAGCAAGAAGAGCGTTGAAACATACAAGATCTACATCTTCAAAGTTCTGAAACAAGTTCACCCAGACATTGGTATCTCAAGCAAAGCCATGGGAATCATGAACAGTTTCATCAACGATATCTTTGAGAAGCTCGCTCAAGAATCTTCCAGACTCGCTAGGTACAACAAGAAGCCGACGATCACTTCAAGGGAAATTCAGACCGCGGTCAGGCTTGTTCTGCCCGGTGAATTGGCCAAGCATGCTGTTTCTGAGGGTACTAAGGCTGTGACTAAGTTCACAAGTTCTTAG

Protein sequence

>MsG0480021302.01.T01

MAPKGEKKPAEKKPAEEKKSTVAEKAPAEKKPKAGKKLPKEGGSAAGDKKKKRSKKSVETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSRLARYNKKPTITSREIQTAVRLVLPGELAKHAVSEGTKAVTKFTSS*