AlfalfaGEDB | MsG0780040844.01

M. sativa cultivar ZhongmuNo.1 / MsG0780040844.01

KEGG: K11254
GO: GO:0000786 GO:0003677 GO:0005634 GO:0006334 GO:0046982
PFAM: PF15511

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780040844.01.T01	KAG2660576.1	100	103	0	0	1	103	28	130	9.14E-66	204

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780040844.01.T01	P0CG89	100	103	0	0	1	103	1	103	3.05E-67	199

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780040844.01.T01	A0A453MZH1	100.000	103	0	0	1	103	31	133	3.54e-66	204

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180002936.01	MsG0780040844.01	0.809107	2.227706e-50	1.296488e-47
MsG0180005048.01	MsG0780040844.01	0.820675	6.051699e-53	4.819639e-50
MsG0780040841.01	MsG0780040844.01	0.816665	4.919803e-52	3.507848e-49
MsG0780040844.01	MsG0780040859.01	0.811257	7.665642e-51	4.722400e-48
MsG0780040844.01	MsG0780041592.01	0.828113	1.076725e-54	1.060184e-51
MsG0780040844.01	MsG0880045145.01	0.801513	8.688857e-49	4.157375e-46
MsG0280011003.01	MsG0780040844.01	0.804515	2.082122e-49	1.075605e-46
MsG0380014289.01	MsG0780040844.01	0.815109	1.094032e-51	7.474728e-49
MsG0380014684.01	MsG0780040844.01	0.829032	6.456372e-55	6.529098e-52
MsG0480020927.01	MsG0780040844.01	0.819011	1.452822e-52	1.104943e-49
MsG0480021016.01	MsG0780040844.01	0.804789	1.824976e-49	9.495557e-47
MsG0480021252.01	MsG0780040844.01	0.805283	1.438602e-49	7.580409e-47
MsG0480021302.01	MsG0780040844.01	0.853526	2.253904e-61	4.884619e-58
MsG0480022982.01	MsG0780040844.01	0.831474	1.635486e-55	1.776797e-52
MsG0380015234.01	MsG0780040844.01	0.867129	1.718601e-65	5.933703e-62

PPI

Gene1	Gene2	Type
MsG0780040844.01	MsG0880043592.01	PPI
MsG0780040844.01	MsG0880047472.01	PPI
MsG0780040844.01	MsG0780037240.01	PPI
MsG0780040844.01	MsG0580027650.01	PPI
MsG0780040844.01	MsG0880045145.01	PPI
MsG0780040844.01	MsG0480020879.01	PPI
MsG0780040844.01	MsG0380014681.01	PPI
MsG0880047473.01	MsG0780040844.01	PPI
MsG0780040844.01	MsG0780041535.01	PPI
MsG0880045145.01	MsG0780040844.01	PPI
MsG0780040844.01	MsG0380014684.01	PPI
MsG0580027650.01	MsG0780040844.01	PPI
MsG0780037240.01	MsG0780040844.01	PPI
MsG0780040844.01	MsG0380015234.01	PPI
MsG0780040844.01	MsG0580027651.01	PPI
MsG0780040844.01	MsG0880047470.01	PPI
MsG0780040844.01	MsG0880047473.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780040844.01.T01	MTR_7g029510	100.000	103	0	1	103	1	103	2.97e-68	199
MsG0780040844.01.T01	MTR_7g099610	100.000	103	0	1	103	1	103	2.97e-68	199
MsG0780040844.01.T01	MTR_5g063620	100.000	103	0	1	103	1	103	2.97e-68	199
MsG0780040844.01.T01	MTR_4g061827	100.000	103	0	1	103	1	103	2.97e-68	199
MsG0780040844.01.T01	MTR_4g061920	100.000	103	0	1	103	1	103	2.97e-68	199
MsG0780040844.01.T01	MTR_3g462400	100.000	103	0	1	103	1	103	2.97e-68	199
MsG0780040844.01.T01	MTR_3g054380	100.000	103	0	1	103	1	103	2.97e-68	199
MsG0780040844.01.T01	MTR_3g462730	100.000	103	0	1	103	1	103	2.97e-68	199
MsG0780040844.01.T01	MTR_3g462510	100.000	103	0	1	103	1	103	2.97e-68	199
MsG0780040844.01.T01	MTR_3g070920	100.000	103	0	1	103	1	103	2.97e-68	199
MsG0780040844.01.T01	MTR_3g061440	100.000	103	0	1	103	1	103	2.97e-68	199
MsG0780040844.01.T01	MTR_3g462410	100.000	103	0	1	103	1	103	2.97e-68	199
MsG0780040844.01.T01	MTR_2g096100	100.000	103	0	1	103	1	103	2.97e-68	199
MsG0780040844.01.T01	MTR_8g038460	100.000	103	0	1	103	1	103	2.97e-68	199
MsG0780040844.01.T01	MTR_4g128150	100.000	102	0	1	102	1	102	1.74e-66	204
MsG0780040844.01.T01	MTR_7g029520	84.000	50	8	27	76	1	50	2.23e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780040844.01.T01	AT5G59970	100.000	103	0	1	103	1	103	3.11e-68	199
MsG0780040844.01.T01	AT5G59690	100.000	103	0	1	103	1	103	3.11e-68	199
MsG0780040844.01.T01	AT5G59970	100.000	103	0	1	103	1	103	3.11e-68	199
MsG0780040844.01.T01	AT3G46320	100.000	103	0	1	103	1	103	3.11e-68	199
MsG0780040844.01.T01	AT3G45930	100.000	103	0	1	103	1	103	3.11e-68	199
MsG0780040844.01.T01	AT3G53730	100.000	103	0	1	103	1	103	3.11e-68	199
MsG0780040844.01.T01	AT2G28740	100.000	103	0	1	103	1	103	3.11e-68	199
MsG0780040844.01.T01	AT1G07660	100.000	103	0	1	103	1	103	3.11e-68	199
MsG0780040844.01.T01	AT1G07820	100.000	103	0	1	103	1	103	3.11e-68	199
MsG0780040844.01.T01	AT1G07820	100.000	103	0	1	103	1	103	3.11e-68	199
MsG0780040844.01.T01	AT1G07660	83.495	103	1	1	103	1	86	4.52e-51	155

Find 32 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGGTGTTCTCAAGATCTTCT+TGG	0.324000	7:-83247101	MsG0780040844.01.T01:CDS
GGAAAGGGATTGGGAAAGGG+AGG	0.412951	7:-83247247	MsG0780040844.01.T01:CDS
TCGCAGGCTTTGTTATGCCT+TGG	0.436279	7:+83247188	None:intergenic
AAAATGTCTGGCCGCGGAAA+AGG	0.446222	7:-83247271	MsG0780040844.01.T01:CDS
TGTTTATGCTTTGAAGAGAC+AGG	0.447929	7:-83247008	MsG0780040844.01.T01:CDS
GGAGGAAAGGGATTGGGAAA+GGG	0.462159	7:-83247250	MsG0780040844.01.T01:CDS
GGAAGGACTCTCTACGGTTT+CGG	0.462635	7:-83246986	MsG0780040844.01.T01:CDS
AGGAGGAAAGGGATTGGGAA+AGG	0.466050	7:-83247251	MsG0780040844.01.T01:CDS
GGAAAAGGAGGAAAGGGATT+GGG	0.479340	7:-83247256	MsG0780040844.01.T01:CDS
CGGAAAAGGAGGAAAGGGAT+TGG	0.482086	7:-83247257	MsG0780040844.01.T01:CDS
CTGCGATTCGTCGTCTTGCA+AGG	0.502831	7:-83247171	MsG0780040844.01.T01:CDS
GGCCGCGGAAAAGGAGGAAA+GGG	0.550993	7:-83247262	MsG0780040844.01.T01:CDS
CTTGCAAGACGACGAATCGC+AGG	0.552192	7:+83247172	None:intergenic
AGGAGCAAAGAGGCACAGAA+AGG	0.564264	7:-83247227	MsG0780040844.01.T01:CDS
TTACTTACACTGAGCACGCT+AGG	0.572434	7:-83247057	MsG0780040844.01.T01:CDS
ATGTCTGGCCGCGGAAAAGG+AGG	0.578066	7:-83247268	MsG0780040844.01.T01:CDS
GAGGAAGACTGTTACCGCCA+TGG	0.588179	7:-83247035	MsG0780040844.01.T01:CDS
TGGCCGCGGAAAAGGAGGAA+AGG	0.594714	7:-83247263	MsG0780040844.01.T01:CDS
AGACAGGGAAGGACTCTCTA+CGG	0.604561	7:-83246992	MsG0780040844.01.T01:CDS
AGGACTCTCTACGGTTTCGG+TGG	0.607233	7:-83246983	MsG0780040844.01.T01:CDS
CAAAGCATAAACAACATCCA+TGG	0.612707	7:+83247018	None:intergenic
CGTCGTCTTGCAAGGCGTGG+TGG	0.615268	7:-83247163	MsG0780040844.01.T01:CDS
GTTTATGCTTTGAAGAGACA+GGG	0.618996	7:-83247007	MsG0780040844.01.T01:CDS
ATGCTTTGAAGAGACAGGGA+AGG	0.619457	7:-83247003	MsG0780040844.01.T01:CDS
ATTCGTCGTCTTGCAAGGCG+TGG	0.619919	7:-83247166	MsG0780040844.01.T01:CDS
GTTCTTCGCGATAACATCCA+AGG	0.633409	7:-83247205	MsG0780040844.01.T01:CDS
AGCGTGCTCAGTGTAAGTAA+CGG	0.650649	7:+83247060	None:intergenic
CTTACACTGAGCACGCTAGG+AGG	0.678435	7:-83247054	MsG0780040844.01.T01:CDS
TGGGAAAGGGAGGAGCAAAG+AGG	0.685544	7:-83247237	MsG0780040844.01.T01:CDS
GGTGGTGTGAAGAGAATCAG+TGG	0.694412	7:-83247145	MsG0780040844.01.T01:CDS
CTCATCTATGAAGAGACTCG+CGG	0.697662	7:-83247121	MsG0780040844.01.T01:CDS
AGCATAAACAACATCCATGG+CGG	0.725411	7:+83247021	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	CAAAGCATAAACAACATCCA+TGG	+	Chr7:83247232-83247251	None:intergenic	35.0%
!!	GTTTATGCTTTGAAGAGACA+GGG	-	Chr7:83247240-83247259	MsG0780040844.01.T01:CDS	35.0%
!!	TGTTTATGCTTTGAAGAGAC+AGG	-	Chr7:83247239-83247258	MsG0780040844.01.T01:CDS	35.0%
	AGCATAAACAACATCCATGG+CGG	+	Chr7:83247229-83247248	None:intergenic	40.0%
	AGCGTGCTCAGTGTAAGTAA+CGG	+	Chr7:83247190-83247209	None:intergenic	45.0%
	CGGTGTTCTCAAGATCTTCT+TGG	-	Chr7:83247146-83247165	MsG0780040844.01.T01:CDS	45.0%
	CTCATCTATGAAGAGACTCG+CGG	-	Chr7:83247126-83247145	MsG0780040844.01.T01:CDS	45.0%
	GGAAAAGGAGGAAAGGGATT+GGG	-	Chr7:83246991-83247010	MsG0780040844.01.T01:CDS	45.0%
	GTTCTTCGCGATAACATCCA+AGG	-	Chr7:83247042-83247061	MsG0780040844.01.T01:CDS	45.0%
	TTACTTACACTGAGCACGCT+AGG	-	Chr7:83247190-83247209	MsG0780040844.01.T01:CDS	45.0%
!!	ATGCTTTGAAGAGACAGGGA+AGG	-	Chr7:83247244-83247263	MsG0780040844.01.T01:CDS	45.0%
	AGACAGGGAAGGACTCTCTA+CGG	-	Chr7:83247255-83247274	MsG0780040844.01.T01:CDS	50.0%
	AGGAGGAAAGGGATTGGGAA+AGG	-	Chr7:83246996-83247015	MsG0780040844.01.T01:CDS	50.0%
	CGGAAAAGGAGGAAAGGGAT+TGG	-	Chr7:83246990-83247009	MsG0780040844.01.T01:CDS	50.0%
	GGAAGGACTCTCTACGGTTT+CGG	-	Chr7:83247261-83247280	MsG0780040844.01.T01:CDS	50.0%
	GGAGGAAAGGGATTGGGAAA+GGG	-	Chr7:83246997-83247016	MsG0780040844.01.T01:CDS	50.0%
	GGTGGTGTGAAGAGAATCAG+TGG	-	Chr7:83247102-83247121	MsG0780040844.01.T01:CDS	50.0%
!	AGGAGCAAAGAGGCACAGAA+AGG	-	Chr7:83247020-83247039	MsG0780040844.01.T01:CDS	50.0%
!	ATCCCTTTCCTCCTTTTCCG+CGG	+	Chr7:83246990-83247009	None:intergenic	50.0%
!!	TCGCAGGCTTTGTTATGCCT+TGG	+	Chr7:83247062-83247081	None:intergenic	50.0%
	AGGACTCTCTACGGTTTCGG+TGG	-	Chr7:83247264-83247283	MsG0780040844.01.T01:CDS	55.0%
	ATTCGTCGTCTTGCAAGGCG+TGG	-	Chr7:83247081-83247100	MsG0780040844.01.T01:CDS	55.0%
	CTGCGATTCGTCGTCTTGCA+AGG	-	Chr7:83247076-83247095	MsG0780040844.01.T01:CDS	55.0%
	CTTACACTGAGCACGCTAGG+AGG	-	Chr7:83247193-83247212	MsG0780040844.01.T01:CDS	55.0%
	CTTGCAAGACGACGAATCGC+AGG	+	Chr7:83247078-83247097	None:intergenic	55.0%
	GAGGAAGACTGTTACCGCCA+TGG	-	Chr7:83247212-83247231	MsG0780040844.01.T01:CDS	55.0%
	GGAAAGGGATTGGGAAAGGG+AGG	-	Chr7:83247000-83247019	MsG0780040844.01.T01:CDS	55.0%
	TGGGAAAGGGAGGAGCAAAG+AGG	-	Chr7:83247010-83247029	MsG0780040844.01.T01:CDS	55.0%
	ATGTCTGGCCGCGGAAAAGG+AGG	-	Chr7:83246979-83246998	MsG0780040844.01.T01:CDS	60.0%
	GGCCGCGGAAAAGGAGGAAA+GGG	-	Chr7:83246985-83247004	MsG0780040844.01.T01:CDS	60.0%
	TGGCCGCGGAAAAGGAGGAA+AGG	-	Chr7:83246984-83247003	MsG0780040844.01.T01:CDS	60.0%
	CGTCGTCTTGCAAGGCGTGG+TGG	-	Chr7:83247084-83247103	MsG0780040844.01.T01:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
Chr7	gene	83246979	83247290	83246979	ID=MsG0780040844.01;Name=MsG0780040844.01
Chr7	mRNA	83246979	83247290	83246979	ID=MsG0780040844.01.T01;Parent=MsG0780040844.01;Name=MsG0780040844.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|103
Chr7	exon	83246979	83247290	83246979	ID=MsG0780040844.01.T01:exon:19741;Parent=MsG0780040844.01.T01
Chr7	CDS	83246979	83247290	83246979	ID=MsG0780040844.01.T01:cds;Parent=MsG0780040844.01.T01

Gene Sequence	>MsG0780040844.01.T01 ATGTCTGGCCGCGGAAAAGGAGGAAAGGGATTGGGAAAGGGAGGAGCAAAGAGGCACAGAAAGGTTCTTCGCGATAACATCCAAGGCATAACAAAGCCTGCGATTCGTCGTCTTGCAAGGCGTGGTGGTGTGAAGAGAATCAGTGGTCTCATCTATGAAGAGACTCGCGGTGTTCTCAAGATCTTCTTGGAGAACGTGATTCGTGATGCCGTTACTTACACTGAGCACGCTAGGAGGAAGACTGTTACCGCCATGGATGTTGTTTATGCTTTGAAGAGACAGGGAAGGACTCTCTACGGTTTCGGTGGTTAA
Protein sequence	>MsG0780040844.01.T01 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG*