Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041176.01.T01 | PNX80109.1 | 64.783 | 230 | 49 | 5 | 1 | 198 | 1 | 230 | 5.50E-95 | 286 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041176.01.T01 | Q9FNH6 | 33.058 | 242 | 106 | 7 | 1 | 197 | 1 | 231 | 1.52E-32 | 119 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041176.01.T01 | A0A2K3LNK4 | 64.783 | 230 | 49 | 5 | 1 | 198 | 1 | 230 | 2.63e-95 | 286 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180003248.01 | MsG0780041176.01 | 0.813642 | 2.307762e-51 | 1.515463e-48 |
| MsG0180003249.01 | MsG0780041176.01 | 0.844754 | 6.216667e-59 | 1.013686e-55 |
| MsG0180003250.01 | MsG0780041176.01 | 0.810361 | 1.197724e-50 | 7.204950e-48 |
| MsG0180004328.01 | MsG0780041176.01 | 0.803677 | 3.110727e-49 | 1.572776e-46 |
| MsG0180005636.01 | MsG0780041176.01 | 0.822640 | 2.126356e-53 | 1.790139e-50 |
| MsG0180005648.01 | MsG0780041176.01 | 0.805172 | 1.517621e-49 | 7.974395e-47 |
| MsG0780041176.01 | MsG0780041177.01 | 0.840672 | 7.551399e-58 | 1.083462e-54 |
| MsG0780041176.01 | MsG0780041574.01 | 0.827903 | 1.209922e-54 | 1.183939e-51 |
| MsG0780041176.01 | MsG0880047494.01 | 0.821886 | 3.181416e-53 | 2.621240e-50 |
| MsG0480021697.01 | MsG0780041176.01 | 0.809711 | 1.653349e-50 | 9.778209e-48 |
| MsG0480023349.01 | MsG0780041176.01 | 0.829021 | 6.496997e-55 | 6.568054e-52 |
| MsG0480023658.01 | MsG0780041176.01 | 0.817992 | 2.473579e-52 | 1.828943e-49 |
| MsG0380015459.01 | MsG0780041176.01 | 0.803095 | 4.106134e-49 | 2.045199e-46 |
| MsG0380016481.01 | MsG0780041176.01 | 0.822909 | 1.840474e-53 | 1.561284e-50 |
| MsG0380017859.01 | MsG0780041176.01 | 0.834248 | 3.345643e-56 | 3.947817e-53 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041176.01.T01 | MTR_7g105970 | 70.435 | 230 | 33 | 5 | 1 | 198 | 1 | 227 | 1.49e-95 | 277 |
| MsG0780041176.01.T01 | MTR_7g106000 | 63.043 | 230 | 50 | 6 | 1 | 198 | 1 | 227 | 3.44e-91 | 266 |
| MsG0780041176.01.T01 | MTR_7g106010 | 56.637 | 226 | 64 | 6 | 6 | 197 | 2 | 227 | 1.47e-77 | 232 |
| MsG0780041176.01.T01 | MTR_7g106030 | 55.895 | 229 | 66 | 6 | 4 | 197 | 3 | 231 | 1.53e-73 | 222 |
| MsG0780041176.01.T01 | MTR_1g057400 | 56.944 | 144 | 60 | 2 | 55 | 197 | 67 | 209 | 3.53e-50 | 161 |
| MsG0780041176.01.T01 | MTR_1g057290 | 51.825 | 137 | 65 | 1 | 62 | 197 | 81 | 217 | 5.13e-39 | 133 |
| MsG0780041176.01.T01 | MTR_1g057270 | 40.000 | 160 | 90 | 3 | 40 | 197 | 87 | 242 | 5.59e-31 | 113 |
| MsG0780041176.01.T01 | MTR_4g119428 | 30.204 | 245 | 115 | 7 | 1 | 196 | 2 | 239 | 2.53e-27 | 104 |
| MsG0780041176.01.T01 | MTR_7g105870 | 40.602 | 133 | 74 | 3 | 65 | 196 | 99 | 227 | 1.07e-22 | 91.7 |
| MsG0780041176.01.T01 | MTR_2g048270 | 33.775 | 151 | 87 | 8 | 48 | 193 | 2 | 144 | 3.42e-12 | 62.4 |
| MsG0780041176.01.T01 | MTR_1g057300 | 60.417 | 48 | 18 | 1 | 54 | 101 | 64 | 110 | 1.26e-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041176.01.T01 | AT5G06320 | 33.058 | 242 | 106 | 7 | 1 | 197 | 1 | 231 | 1.55e-33 | 119 |
| MsG0780041176.01.T01 | AT2G35980 | 31.004 | 229 | 117 | 6 | 5 | 197 | 4 | 227 | 5.48e-31 | 113 |
| MsG0780041176.01.T01 | AT2G35460 | 40.541 | 148 | 82 | 3 | 51 | 197 | 96 | 238 | 9.58e-29 | 107 |
| MsG0780041176.01.T01 | AT3G11650 | 30.579 | 242 | 115 | 7 | 4 | 197 | 3 | 239 | 1.26e-27 | 104 |
| MsG0780041176.01.T01 | AT1G32270 | 30.303 | 132 | 87 | 2 | 54 | 184 | 24 | 151 | 1.61e-12 | 65.5 |
| MsG0780041176.01.T01 | AT1G32270 | 30.303 | 132 | 87 | 2 | 54 | 184 | 24 | 151 | 2.65e-12 | 65.1 |
Find 40 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATCACTTCTCTTCTTAAAT+TGG | 0.196139 | 7:+87642736 | None:intergenic |
| GCTGTCTCTTCAGTTTCTTC+TGG | 0.267585 | 7:-87643028 | MsG0780041176.01.T01:CDS |
| AACAAACAACCACAATTAAA+TGG | 0.271533 | 7:-87643127 | MsG0780041176.01.T01:CDS |
| AGACTCGGGAATGTGATATT+TGG | 0.318840 | 7:-87642588 | MsG0780041176.01.T01:CDS |
| TGTGCTGGTGGTGGAATGGC+GGG | 0.331387 | 7:+87643091 | None:intergenic |
| TAATTGTGGTTGTTTGTTAT+CGG | 0.339443 | 7:+87643132 | None:intergenic |
| TTGAGGTCACATTTCACTTT+CGG | 0.367481 | 7:+87642555 | None:intergenic |
| TGTTTCTGTAGTCGTTAGAT+TGG | 0.389981 | 7:+87642910 | None:intergenic |
| GTGAGGTGGTGAGGTTGTGC+TGG | 0.399692 | 7:+87643076 | None:intergenic |
| TGGTGAGGTTGTGCTGGTGG+TGG | 0.412702 | 7:+87643082 | None:intergenic |
| AGGTGGTGAGGTTGTGCTGG+TGG | 0.419177 | 7:+87643079 | None:intergenic |
| AGGTTCAAGGTTCGCGGATG+TGG | 0.425570 | 7:-87642787 | MsG0780041176.01.T01:CDS |
| AAATTGGCGGAATGAATTCA+TGG | 0.426566 | 7:+87642752 | None:intergenic |
| CACTTCTCTTCTTAAATTGG+CGG | 0.426788 | 7:+87642739 | None:intergenic |
| AGGTTGTGCTGGTGGTGGAA+TGG | 0.466852 | 7:+87643087 | None:intergenic |
| TGGAATGGCGGGGCCGTAGT+AGG | 0.474577 | 7:+87643102 | None:intergenic |
| CAATTAAATGGTGCCTACTA+CGG | 0.482964 | 7:-87643115 | MsG0780041176.01.T01:CDS |
| CCTGAGAAAACACCACTCAT+AGG | 0.484020 | 7:+87642714 | None:intergenic |
| GAGCATTCTATGAAGGTTCA+AGG | 0.492970 | 7:-87642800 | MsG0780041176.01.T01:CDS |
| TAGAACTCAAAGGAACTTTG+AGG | 0.493608 | 7:+87642538 | None:intergenic |
| TTGTGCTGGTGGTGGAATGG+CGG | 0.506260 | 7:+87643090 | None:intergenic |
| CTATGAAGGTTCAAGGTTCG+CGG | 0.520781 | 7:-87642793 | MsG0780041176.01.T01:CDS |
| TTGAACCTTCATAGAATGCT+CGG | 0.521945 | 7:+87642802 | None:intergenic |
| GAAATCAACATCACACTTGG+TGG | 0.535038 | 7:+87642482 | None:intergenic |
| GAGGCCCGAGCATTCTATGA+AGG | 0.539846 | 7:-87642807 | MsG0780041176.01.T01:CDS |
| AAGCTGAGTGAAGGTTGCAC+TGG | 0.553930 | 7:+87642509 | None:intergenic |
| TTGCTTCGGTGGCGGTGAGG+TGG | 0.554196 | 7:+87643062 | None:intergenic |
| TCGAAATTAGATTCAGACTC+GGG | 0.556645 | 7:-87642602 | MsG0780041176.01.T01:CDS |
| TGAACCTTCATAGAATGCTC+GGG | 0.564728 | 7:+87642803 | None:intergenic |
| TAGTAGGCACCATTTAATTG+TGG | 0.569855 | 7:+87643118 | None:intergenic |
| TCGGTGGCGGTGAGGTGGTG+AGG | 0.575116 | 7:+87643067 | None:intergenic |
| CTGGTATTCTTAGAACTCAA+AGG | 0.578974 | 7:+87642528 | None:intergenic |
| TTCGAAATTAGATTCAGACT+CGG | 0.596801 | 7:-87642603 | MsG0780041176.01.T01:CDS |
| AAATCAACATCACACTTGGT+GGG | 0.599036 | 7:+87642483 | None:intergenic |
| GTGCTGGTGGTGGAATGGCG+GGG | 0.625212 | 7:+87643092 | None:intergenic |
| CTTCTATTACGACAAAGTTG+AGG | 0.634945 | 7:-87642826 | MsG0780041176.01.T01:CDS |
| CAACACACTACATTACAACA+TGG | 0.639018 | 7:-87642889 | MsG0780041176.01.T01:CDS |
| GTAGAAATCAACATCACACT+TGG | 0.671261 | 7:+87642479 | None:intergenic |
| AAGAGAAGTGATCCTATGAG+TGG | 0.676717 | 7:-87642726 | MsG0780041176.01.T01:CDS |
| GGTGGGCACAAGCTGAGTGA+AGG | 0.685346 | 7:+87642500 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AACAAACAACCACAATTAAA+TGG | - | Chr7:87642485-87642504 | MsG0780041176.01.T01:CDS | 25.0% |
| !!! | AGAAGTTGAGTTTTTTGTTT+GGG | + | Chr7:87642771-87642790 | None:intergenic | 25.0% |
| !!! | TAATTGTGGTTGTTTGTTAT+CGG | + | Chr7:87642483-87642502 | None:intergenic | 25.0% |
| !!! | TAGAAGTTGAGTTTTTTGTT+TGG | + | Chr7:87642772-87642791 | None:intergenic | 25.0% |
| GAACAATGAGATAGAAGATA+AGG | + | Chr7:87642649-87642668 | None:intergenic | 30.0% | |
| GATCACTTCTCTTCTTAAAT+TGG | + | Chr7:87642879-87642898 | None:intergenic | 30.0% | |
| TTCGAAATTAGATTCAGACT+CGG | - | Chr7:87643009-87643028 | MsG0780041176.01.T01:intron | 30.0% | |
| AAATCAACATCACACTTGGT+GGG | + | Chr7:87643132-87643151 | None:intergenic | 35.0% | |
| AAATTGGCGGAATGAATTCA+TGG | + | Chr7:87642863-87642882 | None:intergenic | 35.0% | |
| CAACACACTACATTACAACA+TGG | - | Chr7:87642723-87642742 | MsG0780041176.01.T01:CDS | 35.0% | |
| CAATTAAATGGTGCCTACTA+CGG | - | Chr7:87642497-87642516 | MsG0780041176.01.T01:CDS | 35.0% | |
| CACTTCTCTTCTTAAATTGG+CGG | + | Chr7:87642876-87642895 | None:intergenic | 35.0% | |
| CTGGTATTCTTAGAACTCAA+AGG | + | Chr7:87643087-87643106 | None:intergenic | 35.0% | |
| CTTCTATTACGACAAAGTTG+AGG | - | Chr7:87642786-87642805 | MsG0780041176.01.T01:CDS | 35.0% | |
| GTAGAAATCAACATCACACT+TGG | + | Chr7:87643136-87643155 | None:intergenic | 35.0% | |
| TAGAACTCAAAGGAACTTTG+AGG | + | Chr7:87643077-87643096 | None:intergenic | 35.0% | |
| TAGTAGGCACCATTTAATTG+TGG | + | Chr7:87642497-87642516 | None:intergenic | 35.0% | |
| TCGAAATTAGATTCAGACTC+GGG | - | Chr7:87643010-87643029 | MsG0780041176.01.T01:intron | 35.0% | |
| TTCGTTGACATAGAACTTGA+AGG | + | Chr7:87642681-87642700 | None:intergenic | 35.0% | |
| TTGAACCTTCATAGAATGCT+CGG | + | Chr7:87642813-87642832 | None:intergenic | 35.0% | |
| TTGAGGTCACATTTCACTTT+CGG | + | Chr7:87643060-87643079 | None:intergenic | 35.0% | |
| ! | TGTTTCTGTAGTCGTTAGAT+TGG | + | Chr7:87642705-87642724 | None:intergenic | 35.0% |
| AAGAGAAGTGATCCTATGAG+TGG | - | Chr7:87642886-87642905 | MsG0780041176.01.T01:CDS | 40.0% | |
| AGACTCGGGAATGTGATATT+TGG | - | Chr7:87643024-87643043 | MsG0780041176.01.T01:CDS | 40.0% | |
| GAAATCAACATCACACTTGG+TGG | + | Chr7:87643133-87643152 | None:intergenic | 40.0% | |
| GAGCATTCTATGAAGGTTCA+AGG | - | Chr7:87642812-87642831 | MsG0780041176.01.T01:CDS | 40.0% | |
| TGAACCTTCATAGAATGCTC+GGG | + | Chr7:87642812-87642831 | None:intergenic | 40.0% | |
| ! | ACATAGAACTTGAAGGCATG+AGG | + | Chr7:87642674-87642693 | None:intergenic | 40.0% |
| ! | AGCAACAACAGCTTTTGCTT+CGG | + | Chr7:87642567-87642586 | None:intergenic | 40.0% |
| ! | CTATGAGTGGTGTTTTCTCA+GGG | - | Chr7:87642899-87642918 | MsG0780041176.01.T01:CDS | 40.0% |
| !!! | TTTTTTGTTTGGGTTGCGAG+CGG | + | Chr7:87642761-87642780 | None:intergenic | 40.0% |
| CCTGAGAAAACACCACTCAT+AGG | + | Chr7:87642901-87642920 | None:intergenic | 45.0% | |
| CTATGAAGGTTCAAGGTTCG+CGG | - | Chr7:87642819-87642838 | MsG0780041176.01.T01:CDS | 45.0% | |
| GCTGTCTCTTCAGTTTCTTC+TGG | - | Chr7:87642584-87642603 | MsG0780041176.01.T01:CDS | 45.0% | |
| ! | AACAACAGCTTTTGCTTCGG+TGG | + | Chr7:87642564-87642583 | None:intergenic | 45.0% |
| ! | CCTATGAGTGGTGTTTTCTC+AGG | - | Chr7:87642898-87642917 | MsG0780041176.01.T01:CDS | 45.0% |
| AAGCTGAGTGAAGGTTGCAC+TGG | + | Chr7:87643106-87643125 | None:intergenic | 50.0% | |
| ! | AACAGCTTTTGCTTCGGTGG+CGG | + | Chr7:87642561-87642580 | None:intergenic | 50.0% |
| ! | GTGGTGTTTTCTCAGGGCAA+CGG | - | Chr7:87642905-87642924 | MsG0780041176.01.T01:CDS | 50.0% |
| AGGTTCAAGGTTCGCGGATG+TGG | - | Chr7:87642825-87642844 | MsG0780041176.01.T01:CDS | 55.0% | |
| GAGGCCCGAGCATTCTATGA+AGG | - | Chr7:87642805-87642824 | MsG0780041176.01.T01:CDS | 55.0% | |
| !! | AGGTTGTGCTGGTGGTGGAA+TGG | + | Chr7:87642528-87642547 | None:intergenic | 55.0% |
| !! | TTGTGCTGGTGGTGGAATGG+CGG | + | Chr7:87642525-87642544 | None:intergenic | 55.0% |
| AGGTGGTGAGGTTGTGCTGG+TGG | + | Chr7:87642536-87642555 | None:intergenic | 60.0% | |
| GGTGGGCACAAGCTGAGTGA+AGG | + | Chr7:87643115-87643134 | None:intergenic | 60.0% | |
| GTGAGGTGGTGAGGTTGTGC+TGG | + | Chr7:87642539-87642558 | None:intergenic | 60.0% | |
| ! | TGGTGAGGTTGTGCTGGTGG+TGG | + | Chr7:87642533-87642552 | None:intergenic | 60.0% |
| !! | CTTTTGCTTCGGTGGCGGTG+AGG | + | Chr7:87642556-87642575 | None:intergenic | 60.0% |
| !! | TGTGCTGGTGGTGGAATGGC+GGG | + | Chr7:87642524-87642543 | None:intergenic | 60.0% |
| ! | TGGAATGGCGGGGCCGTAGT+AGG | + | Chr7:87642513-87642532 | None:intergenic | 65.0% |
| ! | TTGCTTCGGTGGCGGTGAGG+TGG | + | Chr7:87642553-87642572 | None:intergenic | 65.0% |
| !! | GTGCTGGTGGTGGAATGGCG+GGG | + | Chr7:87642523-87642542 | None:intergenic | 65.0% |
| ! | TCGGTGGCGGTGAGGTGGTG+AGG | + | Chr7:87642548-87642567 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 87642476 | 87643158 | 87642476 | ID=MsG0780041176.01;Name=MsG0780041176.01 |
| Chr7 | mRNA | 87642476 | 87643158 | 87642476 | ID=MsG0780041176.01.T01;Parent=MsG0780041176.01;Name=MsG0780041176.01.T01;_AED=0.46;_eAED=0.46;_QI=0|0|0|1|1|1|2|0|198 |
| Chr7 | exon | 87643019 | 87643158 | 87643019 | ID=MsG0780041176.01.T01:exon:20451;Parent=MsG0780041176.01.T01 |
| Chr7 | exon | 87642476 | 87642932 | 87642476 | ID=MsG0780041176.01.T01:exon:20450;Parent=MsG0780041176.01.T01 |
| Chr7 | CDS | 87643019 | 87643158 | 87643019 | ID=MsG0780041176.01.T01:cds;Parent=MsG0780041176.01.T01 |
| Chr7 | CDS | 87642476 | 87642932 | 87642476 | ID=MsG0780041176.01.T01:cds;Parent=MsG0780041176.01.T01 |
| Gene Sequence |
| Protein sequence |
