AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880043837.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043837.01.T02 MTR_8g037923 67.399 273 76 2 1 260 6 278 1.29e-130 378
MsG0880043837.01.T02 MTR_8g042170 67.041 267 79 4 1 260 1 265 2.23e-124 362
MsG0880043837.01.T02 MTR_8g042080 80.000 170 34 0 1 170 1 170 2.51e-102 295
MsG0880043837.01.T02 MTR_8g037920 64.384 219 67 1 46 253 6 224 5.70e-99 295
MsG0880043837.01.T02 MTR_8g042400 58.710 155 60 3 107 260 1 152 1.38e-54 179
MsG0880043837.01.T02 MTR_7g060320 33.582 268 135 7 1 237 7 262 6.58e-38 138
MsG0880043837.01.T02 MTR_7g060310 39.362 188 93 3 1 179 8 183 1.59e-37 138
MsG0880043837.01.T02 MTR_7g059810 36.585 246 120 9 24 243 26 261 8.63e-37 135
MsG0880043837.01.T02 MTR_7g059515 39.362 188 90 6 1 179 6 178 6.57e-36 127
MsG0880043837.01.T02 MTR_7g060115 33.992 253 124 8 20 237 21 265 1.46e-35 132
MsG0880043837.01.T02 MTR_7g060020 40.828 169 83 4 20 179 25 185 1.70e-35 131
MsG0880043837.01.T02 MTR_7g446270 34.553 246 116 9 20 230 25 260 2.68e-35 132
MsG0880043837.01.T02 MTR_0182s0060 33.700 273 136 9 1 233 7 274 6.21e-35 131
MsG0880043837.01.T02 MTR_7g060370 38.220 191 92 6 1 179 7 183 7.73e-34 127
MsG0880043837.01.T02 MTR_7g059540 38.660 194 91 6 1 180 7 186 1.44e-32 124
MsG0880043837.01.T02 MTR_7g059540 38.660 194 91 6 1 180 8 187 1.52e-32 124
MsG0880043837.01.T02 MTR_7g059480 39.759 166 82 5 24 179 25 182 9.52e-32 123
MsG0880043837.01.T02 MTR_7g060260 40.000 170 85 4 20 180 22 183 1.42e-31 120
MsG0880043837.01.T02 MTR_7g060840 37.696 191 95 6 1 179 7 185 1.73e-31 120
MsG0880043837.01.T02 MTR_7g060220 33.906 233 115 7 20 227 21 239 1.53e-29 115
MsG0880043837.01.T02 MTR_7g060330 33.471 242 121 9 22 238 28 254 1.82e-29 115
MsG0880043837.01.T02 MTR_7g060060 39.716 141 68 4 48 179 1 133 1.01e-24 98.6
MsG0880043837.01.T02 MTR_7g060160 29.958 237 119 6 1 227 6 205 1.69e-23 98.6
MsG0880043837.01.T02 MTR_1g048070 32.967 182 84 5 1 175 7 157 1.45e-21 90.5
MsG0880043837.01.T02 MTR_7g059510 35.484 155 61 3 15 169 20 135 1.03e-19 88.2
MsG0880043837.01.T02 MTR_7g059475 36.036 111 62 2 78 179 2 112 1.91e-15 74.3
MsG0880043837.01.T02 MTR_7g060200 35.455 110 52 4 69 169 1 100 7.09e-13 66.2
MsG0880043837.01.T01 MTR_8g037923 67.273 275 77 2 7 268 4 278 7.17e-132 382
MsG0880043837.01.T01 MTR_8g042170 67.041 267 79 4 9 268 1 265 1.92e-124 362
MsG0880043837.01.T01 MTR_8g042080 80.000 170 34 0 9 178 1 170 2.26e-102 295
MsG0880043837.01.T01 MTR_8g037920 64.384 219 67 1 54 261 6 224 5.93e-99 295
MsG0880043837.01.T01 MTR_8g042400 58.710 155 60 3 115 268 1 152 1.60e-54 179
MsG0880043837.01.T01 MTR_7g060310 39.474 190 94 3 7 187 6 183 3.64e-38 140
MsG0880043837.01.T01 MTR_7g060320 33.582 268 135 7 9 245 7 262 7.42e-38 139
MsG0880043837.01.T01 MTR_7g059810 36.585 246 120 9 32 251 26 261 8.30e-37 135
MsG0880043837.01.T01 MTR_7g059515 40.526 190 88 7 7 187 5 178 5.01e-36 128
MsG0880043837.01.T01 MTR_7g446270 37.019 208 104 7 7 201 5 198 1.08e-35 133
MsG0880043837.01.T01 MTR_7g060115 33.992 253 124 8 28 245 21 265 1.20e-35 132
MsG0880043837.01.T01 MTR_7g060020 40.828 169 83 4 28 187 25 185 1.87e-35 132
MsG0880043837.01.T01 MTR_0182s0060 34.432 273 134 10 9 241 7 274 8.61e-35 131
MsG0880043837.01.T01 MTR_7g060370 38.342 193 93 6 7 187 5 183 2.12e-34 129
MsG0880043837.01.T01 MTR_7g059540 38.776 196 92 6 7 188 5 186 3.59e-33 126
MsG0880043837.01.T01 MTR_7g059540 38.776 196 92 6 7 188 6 187 3.78e-33 126
MsG0880043837.01.T01 MTR_7g060260 38.542 192 96 6 6 188 5 183 8.90e-32 122
MsG0880043837.01.T01 MTR_7g060840 37.824 193 96 6 7 187 5 185 9.03e-32 122
MsG0880043837.01.T01 MTR_7g059480 39.759 166 82 5 32 187 25 182 1.20e-31 123
MsG0880043837.01.T01 MTR_7g060330 33.471 242 121 9 30 246 28 254 2.07e-29 116
MsG0880043837.01.T01 MTR_7g060220 33.906 233 115 7 28 235 21 239 2.21e-29 115
MsG0880043837.01.T01 MTR_7g060060 39.716 141 68 4 56 187 1 133 1.06e-24 98.6
MsG0880043837.01.T01 MTR_7g060160 30.833 240 118 7 6 235 4 205 1.15e-23 99.4
MsG0880043837.01.T01 MTR_1g048070 32.973 185 86 5 6 183 4 157 3.48e-22 92.4
MsG0880043837.01.T01 MTR_7g059510 35.484 155 61 3 23 177 20 135 1.14e-19 88.2
MsG0880043837.01.T01 MTR_7g059475 36.036 111 62 2 86 187 2 112 2.20e-15 74.3
MsG0880043837.01.T01 MTR_7g060200 35.455 110 52 4 77 177 1 100 7.29e-13 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 43 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGTTCTTTGATACACTTTA+TGG 0.141064 8:+31442068 MsG0880043837.01.T01:CDS
CATTACAATCATCTTGATTT+AGG 0.149428 8:-31442413 None:intergenic
TTAGAAACCCTTTGAATCTT+AGG 0.176823 8:-31441751 None:intergenic
TCAATATTTGTTGAAGAATA+TGG 0.271682 8:+31441895 MsG0880043837.01.T01:CDS
TAGATACCATTATGGATAAA+AGG 0.284378 8:+31441671 MsG0880043837.01.T01:CDS
ACTTTACTTGACATCCAATA+TGG 0.289356 8:+31442344 MsG0880043837.01.T01:CDS
TCCTTCATTGATGTCCATAT+TGG 0.304215 8:-31442358 None:intergenic
TAACAAAGCATGTAAACTTT+TGG 0.305190 8:+31441939 MsG0880043837.01.T01:CDS
TGGACTTGAGATATTGTTAT+AGG 0.306408 8:+31442226 MsG0880043837.01.T01:CDS
GCATCAATTGGTCATACTTA+AGG 0.314903 8:-31442125 None:intergenic
GGACCAAATGATATAATCTT+AGG 0.321680 8:-31441814 None:intergenic
AACGGTAAAGATTGTTCAAA+TGG 0.332688 8:-31442297 None:intergenic
TTATAGGAATTATCTCAGTA+TGG 0.369917 8:+31442242 MsG0880043837.01.T01:CDS
GAGAGCATTACAAGCTACTA+TGG 0.406521 8:+31441869 MsG0880043837.01.T01:CDS
CTCTAAACATATGCATCAAT+TGG 0.416193 8:-31442137 None:intergenic
TACTTAAGGTTTAGTGTTTG+AGG 0.419939 8:-31442111 None:intergenic
GTTTAGAGATGCTATGTTGT+TGG 0.424654 8:+31442152 MsG0880043837.01.T01:CDS
AGAGAAACTAGATACCATTA+TGG 0.426934 8:+31441663 MsG0880043837.01.T01:CDS
ACACCTAAGATTATATCATT+TGG 0.427896 8:+31441811 MsG0880043837.01.T01:CDS
TGAAGGAAATGAACACAATC+AGG 0.460743 8:+31442374 MsG0880043837.01.T01:CDS
TTTCAATTCCTAAGATTCAA+AGG 0.467119 8:+31441743 MsG0880043837.01.T01:CDS
ACAATAATAATGATATTGCT+TGG 0.471525 8:+31442028 MsG0880043837.01.T01:CDS
TTCAATTCCTAAGATTCAAA+GGG 0.485063 8:+31441744 MsG0880043837.01.T01:CDS
GGTATCTAGTTTCTCTGCCA+TGG 0.488995 8:-31441656 None:intergenic
TTCACTGAGGATGCAATTGA+AGG 0.489786 8:+31442003 MsG0880043837.01.T01:CDS
TCCAATATGGACATCAATGA+AGG 0.500777 8:+31442357 MsG0880043837.01.T01:CDS
AATAATAAAAGTCTGAAAGA+AGG 0.509557 8:+31441847 MsG0880043837.01.T01:CDS
ACGGTAAAGATTGTTCAAAT+GGG 0.525435 8:-31442296 None:intergenic
AAGGATTTCCCAACTGAAAG+AGG 0.526037 8:+31441690 MsG0880043837.01.T01:CDS
GGTTTAGTGTTTGAGGACAT+TGG 0.535592 8:-31442104 None:intergenic
GGAGATTATATGCAAAGAGA+AGG 0.536800 8:+31442200 MsG0880043837.01.T01:CDS
TCAACTTCCAAGGAAAATGT+TGG 0.538503 8:+31442179 MsG0880043837.01.T01:CDS
TATAGGAATTATCTCAGTAT+GGG 0.556465 8:+31442243 MsG0880043837.01.T01:CDS
TGTTGGAGAATCAACTTCCA+AGG 0.560053 8:+31442169 MsG0880043837.01.T01:CDS
GAGATTATATGCAAAGAGAA+GGG 0.579002 8:+31442201 MsG0880043837.01.T01:CDS
GGAAATGAACACAATCAGGT+GGG 0.585303 8:+31442378 MsG0880043837.01.T01:CDS
ACTGAATCAAGTATACGAAA+CGG 0.604678 8:-31442315 None:intergenic
ATGGGTGAATGCAAACGACA+TGG 0.614443 8:+31442261 MsG0880043837.01.T01:CDS
AGGAAATGAACACAATCAGG+TGG 0.629310 8:+31442377 MsG0880043837.01.T01:CDS
GAAATGAACACAATCAGGTG+GGG 0.647588 8:+31442379 MsG0880043837.01.T01:CDS
AACTGAAAGAGGCAAAACAA+AGG 0.653451 8:+31441701 MsG0880043837.01.T01:CDS
TATATGCAAAGAGAAGGGTG+TGG 0.675111 8:+31442206 MsG0880043837.01.T01:CDS
TTTGAAGAATATGTTCACTG+AGG 0.714146 8:+31441990 MsG0880043837.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAATAAAAGTCTGAAAGA+AGG + Chr8:31441847-31441866 MsG0880043837.01.T01:CDS 20.0%
!! ACAATAATAATGATATTGCT+TGG + Chr8:31442028-31442047 MsG0880043837.01.T01:CDS 20.0%
!!! CTTTTATTATTGTGATGAAT+AGG - Chr8:31441838-31441857 None:intergenic 20.0%
!!! TCAATATTTGTTGAAGAATA+TGG + Chr8:31441895-31441914 MsG0880043837.01.T01:CDS 20.0%
! ACACCTAAGATTATATCATT+TGG + Chr8:31441811-31441830 MsG0880043837.01.T01:CDS 25.0%
! ATGTTCTTTGATACACTTTA+TGG + Chr8:31442068-31442087 MsG0880043837.01.T01:CDS 25.0%
! CATTACAATCATCTTGATTT+AGG - Chr8:31442416-31442435 None:intergenic 25.0%
! TAGATACCATTATGGATAAA+AGG + Chr8:31441671-31441690 MsG0880043837.01.T01:CDS 25.0%
! TATAGGAATTATCTCAGTAT+GGG + Chr8:31442243-31442262 MsG0880043837.01.T01:CDS 25.0%
! TTATAGGAATTATCTCAGTA+TGG + Chr8:31442242-31442261 MsG0880043837.01.T01:CDS 25.0%
! TTCAATTCCTAAGATTCAAA+GGG + Chr8:31441744-31441763 MsG0880043837.01.T01:CDS 25.0%
! TTTCAATTCCTAAGATTCAA+AGG + Chr8:31441743-31441762 MsG0880043837.01.T01:CDS 25.0%
!! TAACAAAGCATGTAAACTTT+TGG + Chr8:31441939-31441958 MsG0880043837.01.T01:CDS 25.0%
!!! ATGACGAGCTAAATTTTAAT+TGG + Chr8:31442433-31442452 MsG0880043837.01.T01:CDS 25.0%
AACGGTAAAGATTGTTCAAA+TGG - Chr8:31442300-31442319 None:intergenic 30.0%
ACGGTAAAGATTGTTCAAAT+GGG - Chr8:31442299-31442318 None:intergenic 30.0%
ACTGAATCAAGTATACGAAA+CGG - Chr8:31442318-31442337 None:intergenic 30.0%
ACTTTACTTGACATCCAATA+TGG + Chr8:31442344-31442363 MsG0880043837.01.T01:CDS 30.0%
AGAGAAACTAGATACCATTA+TGG + Chr8:31441663-31441682 MsG0880043837.01.T01:CDS 30.0%
CTCTAAACATATGCATCAAT+TGG - Chr8:31442140-31442159 None:intergenic 30.0%
GAGATTATATGCAAAGAGAA+GGG + Chr8:31442201-31442220 MsG0880043837.01.T01:CDS 30.0%
GGACCAAATGATATAATCTT+AGG - Chr8:31441817-31441836 None:intergenic 30.0%
TACTTAAGGTTTAGTGTTTG+AGG - Chr8:31442114-31442133 None:intergenic 30.0%
TGGACTTGAGATATTGTTAT+AGG + Chr8:31442226-31442245 MsG0880043837.01.T01:CDS 30.0%
TTAGAAACCCTTTGAATCTT+AGG - Chr8:31441754-31441773 None:intergenic 30.0%
TTTGAAGAATATGTTCACTG+AGG + Chr8:31441990-31442009 MsG0880043837.01.T01:CDS 30.0%
! ACTTTTTGCGACAAAATGAA+AGG + Chr8:31441773-31441792 MsG0880043837.01.T01:CDS 30.0%
! ATAATCTCCAACATTTTCCT+TGG - Chr8:31442189-31442208 None:intergenic 30.0%
! GGAAATCCTTTTATCCATAA+TGG - Chr8:31441680-31441699 None:intergenic 30.0%
!! TATTGCTTGGATTTTGTTTG+TGG + Chr8:31442041-31442060 MsG0880043837.01.T01:CDS 30.0%
AACTGAAAGAGGCAAAACAA+AGG + Chr8:31441701-31441720 MsG0880043837.01.T01:CDS 35.0%
GCATCAATTGGTCATACTTA+AGG - Chr8:31442128-31442147 None:intergenic 35.0%
GGAGATTATATGCAAAGAGA+AGG + Chr8:31442200-31442219 MsG0880043837.01.T01:CDS 35.0%
GTTTAGAGATGCTATGTTGT+TGG + Chr8:31442152-31442171 MsG0880043837.01.T01:CDS 35.0%
TCAACTTCCAAGGAAAATGT+TGG + Chr8:31442179-31442198 MsG0880043837.01.T01:CDS 35.0%
TCCAATATGGACATCAATGA+AGG + Chr8:31442357-31442376 MsG0880043837.01.T01:CDS 35.0%
TCCTTCATTGATGTCCATAT+TGG - Chr8:31442361-31442380 None:intergenic 35.0%
TGAAGGAAATGAACACAATC+AGG + Chr8:31442374-31442393 MsG0880043837.01.T01:CDS 35.0%
!! TTGTTTTGCCTCTTTCAGTT+GGG - Chr8:31441701-31441720 None:intergenic 35.0%
!!! TTTGTTTTGCCTCTTTCAGT+TGG - Chr8:31441702-31441721 None:intergenic 35.0%
AAGGATTTCCCAACTGAAAG+AGG + Chr8:31441690-31441709 MsG0880043837.01.T01:CDS 40.0%
AGGAAATGAACACAATCAGG+TGG + Chr8:31442377-31442396 MsG0880043837.01.T01:CDS 40.0%
GAAATGAACACAATCAGGTG+GGG + Chr8:31442379-31442398 MsG0880043837.01.T01:CDS 40.0%
GGAAATGAACACAATCAGGT+GGG + Chr8:31442378-31442397 MsG0880043837.01.T01:CDS 40.0%
GGTTTAGTGTTTGAGGACAT+TGG - Chr8:31442107-31442126 None:intergenic 40.0%
TATATGCAAAGAGAAGGGTG+TGG + Chr8:31442206-31442225 MsG0880043837.01.T01:CDS 40.0%
TGTTGGAGAATCAACTTCCA+AGG + Chr8:31442169-31442188 MsG0880043837.01.T01:CDS 40.0%
TTCACTGAGGATGCAATTGA+AGG + Chr8:31442003-31442022 MsG0880043837.01.T01:CDS 40.0%
!! GAGAGCATTACAAGCTACTA+TGG + Chr8:31441869-31441888 MsG0880043837.01.T01:CDS 40.0%
!! GCTTGGATTTTGTTTGTGGA+TGG + Chr8:31442045-31442064 MsG0880043837.01.T01:CDS 40.0%
ATGGGTGAATGCAAACGACA+TGG + Chr8:31442261-31442280 MsG0880043837.01.T01:CDS 45.0%
Chromosome Type Strat End Strand Name
Chr8 gene 31441658 31442464 31441658 ID=MsG0880043837.01;Name=MsG0880043837.01
Chr8 mRNA 31441658 31442464 31441658 ID=MsG0880043837.01.T01;Parent=MsG0880043837.01;Name=MsG0880043837.01.T01;_AED=0.31;_eAED=0.31;_QI=0|-1|0|1|-1|1|1|0|268
Chr8 exon 31441658 31442464 31441658 ID=MsG0880043837.01.T01:exon:9453;Parent=MsG0880043837.01.T01
Chr8 CDS 31441658 31442464 31441658 ID=MsG0880043837.01.T01:cds;Parent=MsG0880043837.01.T01
Chr8 mRNA 31441682 31442464 31441682 ID=MsG0880043837.01.T02;Parent=MsG0880043837.01;Name=MsG0880043837.01.T02;_AED=0.31;_eAED=0.31;_QI=0|-1|0|1|-1|0|1|0|260
Chr8 exon 31441682 31442464 31441682 ID=MsG0880043837.01.T02:exon:9454;Parent=MsG0880043837.01.T02
Chr8 CDS 31441682 31442464 31441682 ID=MsG0880043837.01.T02:cds;Parent=MsG0880043837.01.T02
Gene Sequence

>MsG0880043837.01.T02

ATGGATAAAAGGATTTCCCAACTGAAAGAGGCAAAACAAAGGTTTGATCTAAATAATCAAATTTCAATTCCTAAGATTCAAAGGGTTTCTAACTTTTTGCGACAAAATGAAAGGTTTTATAAGTACTGCACACCTAAGATTATATCATTTGGTCCTATTCATCACAATAATAAAAGTCTGAAAGAAGGAGAGCATTACAAGCTACTATGGACATCAATATTTGTTGAAGAATATGGTCAAAAAATTGATCAAGATGCTAACAAAGCATGTAAACTTTTGGTTCAAAAGATTGAAGATAATATTGAAGATTTGAAGAATATGTTCACTGAGGATGCAATTGAAGGCTACAATAATAATGATATTGCTTGGATTTTGTTTGTGGATGGATGTTCTTTGATACACTTTATGGAAAATATTGATGACCAATGTCCTCAAACACTAAACCTTAAGTATGACCAATTGATGCATATGTTTAGAGATGCTATGTTGTTGGAGAATCAACTTCCAAGGAAAATGTTGGAGATTATATGCAAAGAGAAGGGTGTGGACTTGAGATATTGTTATAGGAATTATCTCAGTATGGGTGAATGCAAACGACATGGAAATGTAGCGATCCCATTTGAACAATCTTTACCGTTTCGTATACTTGATTCAGTTCGCTCACTTTACTTGACATCCAATATGGACATCAATGAAGGAAATGAACACAATCAGGTGGGGAAAAATACAATCCTAAATCAAGATGATTGTAATGACGAGCTAAATTTTAATTGGAATACTTAA

>MsG0880043837.01.T01

ATGGCAGAGAAACTAGATACCATTATGGATAAAAGGATTTCCCAACTGAAAGAGGCAAAACAAAGGTTTGATCTAAATAATCAAATTTCAATTCCTAAGATTCAAAGGGTTTCTAACTTTTTGCGACAAAATGAAAGGTTTTATAAGTACTGCACACCTAAGATTATATCATTTGGTCCTATTCATCACAATAATAAAAGTCTGAAAGAAGGAGAGCATTACAAGCTACTATGGACATCAATATTTGTTGAAGAATATGGTCAAAAAATTGATCAAGATGCTAACAAAGCATGTAAACTTTTGGTTCAAAAGATTGAAGATAATATTGAAGATTTGAAGAATATGTTCACTGAGGATGCAATTGAAGGCTACAATAATAATGATATTGCTTGGATTTTGTTTGTGGATGGATGTTCTTTGATACACTTTATGGAAAATATTGATGACCAATGTCCTCAAACACTAAACCTTAAGTATGACCAATTGATGCATATGTTTAGAGATGCTATGTTGTTGGAGAATCAACTTCCAAGGAAAATGTTGGAGATTATATGCAAAGAGAAGGGTGTGGACTTGAGATATTGTTATAGGAATTATCTCAGTATGGGTGAATGCAAACGACATGGAAATGTAGCGATCCCATTTGAACAATCTTTACCGTTTCGTATACTTGATTCAGTTCGCTCACTTTACTTGACATCCAATATGGACATCAATGAAGGAAATGAACACAATCAGGTGGGGAAAAATACAATCCTAAATCAAGATGATTGTAATGACGAGCTAAATTTTAATTGGAATACTTAA

Protein sequence

>MsG0880043837.01.T02

MDKRISQLKEAKQRFDLNNQISIPKIQRVSNFLRQNERFYKYCTPKIISFGPIHHNNKSLKEGEHYKLLWTSIFVEEYGQKIDQDANKACKLLVQKIEDNIEDLKNMFTEDAIEGYNNNDIAWILFVDGCSLIHFMENIDDQCPQTLNLKYDQLMHMFRDAMLLENQLPRKMLEIICKEKGVDLRYCYRNYLSMGECKRHGNVAIPFEQSLPFRILDSVRSLYLTSNMDINEGNEHNQVGKNTILNQDDCNDELNFNWNT*

>MsG0880043837.01.T01

MAEKLDTIMDKRISQLKEAKQRFDLNNQISIPKIQRVSNFLRQNERFYKYCTPKIISFGPIHHNNKSLKEGEHYKLLWTSIFVEEYGQKIDQDANKACKLLVQKIEDNIEDLKNMFTEDAIEGYNNNDIAWILFVDGCSLIHFMENIDDQCPQTLNLKYDQLMHMFRDAMLLENQLPRKMLEIICKEKGVDLRYCYRNYLSMGECKRHGNVAIPFEQSLPFRILDSVRSLYLTSNMDINEGNEHNQVGKNTILNQDDCNDELNFNWNT*