AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480021056.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021056.01.T01 MTR_4g065017 94.017 117 4 2 1 117 219 332 1.01e-59 186
MsG0480021056.01.T01 MTR_2g097910 40.351 114 38 4 1 112 233 318 7.43e-13 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 14 sgRNAs with CRISPR-Local

Find 16 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGAGTGTAAATATTATTAA+TGG 0.283826 4:-52740077 MsG0480021056.01.T01:CDS
GAGATTTATCTGTTGGAGAT+TGG 0.356328 4:-52739812 MsG0480021056.01.T01:CDS
ATCTGTTGGAGATTGGGATT+TGG 0.391051 4:-52739805 MsG0480021056.01.T01:CDS
AGATTTATCTGTTGGAGATT+GGG 0.394773 4:-52739811 MsG0480021056.01.T01:CDS
AATATTATTAATGGTGTAGA+AGG 0.397124 4:-52740068 MsG0480021056.01.T01:CDS
GAGGTGGAGAATTTATTTCA+TGG 0.406594 4:-52739834 MsG0480021056.01.T01:CDS
TTTCATGGAGATTTATCTGT+TGG 0.445436 4:-52739819 MsG0480021056.01.T01:CDS
TGGTGTAGAAGGAGATGAGA+TGG 0.483693 4:-52740057 MsG0480021056.01.T01:CDS
AATGCATGCAATGGAGAAGA+TGG 0.536091 4:-52739957 MsG0480021056.01.T01:CDS
TAGAGCTGATCATGATGAGG+TGG 0.613651 4:-52739850 MsG0480021056.01.T01:CDS
AAAATGGAGAATGCATGCAA+TGG 0.638345 4:-52739966 MsG0480021056.01.T01:CDS
TTAGTATTAACACTCTCTAG+AGG 0.641496 4:+52740017 None:intergenic
GTATTAACACTCTCTAGAGG+AGG 0.651371 4:+52740020 None:intergenic
AAATAGAGCTGATCATGATG+AGG 0.716489 4:-52739853 MsG0480021056.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATTATTAATGGTGTAGA+AGG - Chr4:52739767-52739786 MsG0480021056.01.T01:CDS 20.0%
! CAGTTTGAAAAATCAAGAAA+TGG + Chr4:52740085-52740104 None:intergenic 25.0%
! TTGTGAACATAGTGTAAAAA+TGG - Chr4:52739853-52739872 MsG0480021056.01.T01:CDS 25.0%
!! GGGAGTGTAAATATTATTAA+TGG - Chr4:52739758-52739777 MsG0480021056.01.T01:CDS 25.0%
AGATTTATCTGTTGGAGATT+GGG - Chr4:52740024-52740043 MsG0480021056.01.T01:CDS 30.0%
TTAGTATTAACACTCTCTAG+AGG + Chr4:52739821-52739840 None:intergenic 30.0%
TTTCATGGAGATTTATCTGT+TGG - Chr4:52740016-52740035 MsG0480021056.01.T01:CDS 30.0%
AAAATGGAGAATGCATGCAA+TGG - Chr4:52739869-52739888 MsG0480021056.01.T01:CDS 35.0%
AAATAGAGCTGATCATGATG+AGG - Chr4:52739982-52740001 MsG0480021056.01.T01:CDS 35.0%
GAGATTTATCTGTTGGAGAT+TGG - Chr4:52740023-52740042 MsG0480021056.01.T01:CDS 35.0%
GAGGTGGAGAATTTATTTCA+TGG - Chr4:52740001-52740020 MsG0480021056.01.T01:CDS 35.0%
AATGCATGCAATGGAGAAGA+TGG - Chr4:52739878-52739897 MsG0480021056.01.T01:CDS 40.0%
ATCTGTTGGAGATTGGGATT+TGG - Chr4:52740030-52740049 MsG0480021056.01.T01:CDS 40.0%
! GTATTAACACTCTCTAGAGG+AGG + Chr4:52739818-52739837 None:intergenic 40.0%
TAGAGCTGATCATGATGAGG+TGG - Chr4:52739985-52740004 MsG0480021056.01.T01:CDS 45.0%
TGGTGTAGAAGGAGATGAGA+TGG - Chr4:52739778-52739797 MsG0480021056.01.T01:CDS 45.0%
Chromosome Type Strat End Strand Name
Chr4 gene 52739752 52740105 52739752 ID=MsG0480021056.01;Name=MsG0480021056.01
Chr4 mRNA 52739752 52740105 52739752 ID=MsG0480021056.01.T01;Parent=MsG0480021056.01;Name=MsG0480021056.01.T01;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|117
Chr4 exon 52739752 52740105 52739752 ID=MsG0480021056.01.T01:exon:20009;Parent=MsG0480021056.01.T01
Chr4 CDS 52739752 52740105 52739752 ID=MsG0480021056.01.T01:cds;Parent=MsG0480021056.01.T01
Gene Sequence

>MsG0480021056.01.T01

ATGTTTGGGAGTGTAAATATTATTAATGGTGTAGAAGGAGATGAGATGGAAATTTACAATGTTCCTCCTCTAGAGAGTGTTAATACTAATAGTACTATTACTTGTGAACATAGTGTAAAAATGGAGAATGCATGCAATGGAGAAGATGGTAATTACAATAGTAGTTATAACTTTGATGACATAAACAACATTGTTATTAACAACTGCAATGTTGTTAGTAAGAGAAGTGAAAATAGAGCTGATCATGATGAGGTGGAGAATTTATTTCATGGAGATTTATCTGTTGGAGATTGGGATTTGGAAGATTTGATGAAAGATGTTTCATCATTTCCATTTCTTGATTTTTCAAACTGA

Protein sequence

>MsG0480021056.01.T01

MFGSVNIINGVEGDEMEIYNVPPLESVNTNSTITCEHSVKMENACNGEDGNYNSSYNFDDINNIVINNCNVVSKRSENRADHDEVENLFHGDLSVGDWDLEDLMKDVSSFPFLDFSN*