AlfalfaGEDB | MsG0880044229.01

M. sativa cultivar ZhongmuNo.1 / MsG0880044229.01

KEGG: -
GO: GO:0009733
PFAM: PF02519

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880044229.01.T01	XP_039685382.1	98.425	127	2	0	1	127	1	127	3.08E-84	252

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880044229.01.T01	Q9C9E1	29.921	127	75	2	1	127	7	119	5.51E-18	76.6

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880044229.01.T01	A0A396GHY8	98.425	127	2	0	1	127	1	127	1.47e-84	252

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0880044229.01	MsG0880045145.01	0.810494	1.120799e-50	6.766065e-48
MsG0880044229.01	MsG0880045965.01	0.818543	1.855814e-52	1.393206e-49
MsG0180003572.01	MsG0880044229.01	0.804730	1.877160e-49	9.752708e-47
MsG0180004854.01	MsG0880044229.01	0.801641	8.181961e-49	3.927389e-46
MsG0180005575.01	MsG0880044229.01	0.801581	8.416795e-49	4.033953e-46
MsG0180006024.01	MsG0880044229.01	0.804104	2.534788e-49	1.295803e-46
MsG0780041259.01	MsG0880044229.01	0.802604	5.185530e-49	2.550853e-46
MsG0680031628.01	MsG0880044229.01	0.810296	1.236688e-50	7.426453e-48
MsG0480022223.01	MsG0880044229.01	0.801276	9.719537e-49	4.622826e-46
MsG0480022696.01	MsG0880044229.01	0.811224	7.791901e-51	4.796053e-48
MsG0580024339.01	MsG0880044229.01	0.806223	9.129171e-50	4.928225e-47
MsG0780037240.01	MsG0880044229.01	0.802492	5.467443e-49	2.681852e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880044229.01.T01	MTR_4g124700	50.000	76	36	1	50	125	47	120	6.37e-22	84.3
MsG0880044229.01.T01	MTR_2g044020	41.463	82	44	2	46	125	45	124	3.34e-20	80.1
MsG0880044229.01.T01	MTR_8g022380	40.964	83	45	1	43	125	36	114	4.99e-12	58.9
MsG0880044229.01.T01	MTR_8g022440	38.554	83	47	1	43	125	38	116	9.47e-11	55.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880044229.01.T01	AT3G12955	48.120	133	59	4	3	126	6	137	1.13e-34	117
MsG0880044229.01.T01	AT1G72430	29.921	127	75	2	1	127	7	119	5.62e-19	76.6
MsG0880044229.01.T01	AT5G20820	37.594	133	69	4	1	126	1	126	3.59e-18	75.1
MsG0880044229.01.T01	AT1G17345	42.857	91	42	3	46	127	42	131	9.79e-18	73.9
MsG0880044229.01.T01	AT1G56150	35.052	97	58	3	12	103	2	98	4.00e-11	56.2

Find 16 sgRNAs with CRISPR-Local

Find 17 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCTCTTTGATCATTTATTA+TGG	0.230139	8:+37990942	MsG0880044229.01.T01:CDS
TTCTGAATCTTTAGAGGAAT+TGG	0.285513	8:+37990994	MsG0880044229.01.T01:CDS
GTTTAGTTCTGAATCTTTAG+AGG	0.292832	8:+37990988	MsG0880044229.01.T01:CDS
GATCAACAAGTCTAAGCAAA+AGG	0.406159	8:+37990877	MsG0880044229.01.T01:CDS
AAGAGCTTGTCAAAGCAATT+AGG	0.412848	8:+37990686	MsG0880044229.01.T01:CDS
TAGTGATAATGAAAATGTTT+TGG	0.436689	8:+37990901	MsG0880044229.01.T01:CDS
CATCATCATATAGTAGCTTA+AGG	0.443310	8:+37990714	MsG0880044229.01.T01:CDS
AATAGAGAAGATTTATATGA+AGG	0.449877	8:+37990746	MsG0880044229.01.T01:CDS
AATTGTGAAACTATATTTGT+TGG	0.453808	8:+37990791	MsG0880044229.01.T01:CDS
AAAGATTCAGAACTAAACTT+AGG	0.498126	8:-37990983	None:intergenic
TTGATCAAAGCTTCAAGAAG+AGG	0.508267	8:-37990860	None:intergenic
CTATATTTGTTGGTAGCTCA+AGG	0.523677	8:+37990801	MsG0880044229.01.T01:CDS
ATCAACAAGTCTAAGCAAAA+GGG	0.536159	8:+37990878	MsG0880044229.01.T01:CDS
TTGTTGGTAGCTCAAGGAAA+CGG	0.544116	8:+37990807	MsG0880044229.01.T01:CDS
ATAGAGAAGATTTATATGAA+GGG	0.553931	8:+37990747	MsG0880044229.01.T01:CDS
GAAGATTTATATGAAGGGCA+TGG	0.610778	8:+37990752	MsG0880044229.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATAGAGAAGATTTATATGA+AGG	+	Chr8:37990746-37990765	MsG0880044229.01.T01:CDS	20.0%
!!	AATTGTGAAACTATATTTGT+TGG	+	Chr8:37990791-37990810	MsG0880044229.01.T01:CDS	20.0%
!!	ATAGAGAAGATTTATATGAA+GGG	+	Chr8:37990747-37990766	MsG0880044229.01.T01:CDS	20.0%
!!	TTCTCTTTGATCATTTATTA+TGG	+	Chr8:37990942-37990961	MsG0880044229.01.T01:CDS	20.0%
!!!	TAGTGATAATGAAAATGTTT+TGG	+	Chr8:37990901-37990920	MsG0880044229.01.T01:CDS	20.0%
!	AAAGATTCAGAACTAAACTT+AGG	-	Chr8:37990986-37991005	None:intergenic	25.0%
	ATCAACAAGTCTAAGCAAAA+GGG	+	Chr8:37990878-37990897	MsG0880044229.01.T01:CDS	30.0%
	CATCATCATATAGTAGCTTA+AGG	+	Chr8:37990714-37990733	MsG0880044229.01.T01:CDS	30.0%
!	TTCTGAATCTTTAGAGGAAT+TGG	+	Chr8:37990994-37991013	MsG0880044229.01.T01:CDS	30.0%
!!	GTTTAGTTCTGAATCTTTAG+AGG	+	Chr8:37990988-37991007	MsG0880044229.01.T01:CDS	30.0%
!!!	TGTTTTGGTTGTTAATTGTG+AGG	+	Chr8:37990916-37990935	MsG0880044229.01.T01:CDS	30.0%
	AAGAGCTTGTCAAAGCAATT+AGG	+	Chr8:37990686-37990705	MsG0880044229.01.T01:CDS	35.0%
	GAAGATTTATATGAAGGGCA+TGG	+	Chr8:37990752-37990771	MsG0880044229.01.T01:CDS	35.0%
	GATCAACAAGTCTAAGCAAA+AGG	+	Chr8:37990877-37990896	MsG0880044229.01.T01:CDS	35.0%
	TTGATCAAAGCTTCAAGAAG+AGG	-	Chr8:37990863-37990882	None:intergenic	35.0%
!!	CTATATTTGTTGGTAGCTCA+AGG	+	Chr8:37990801-37990820	MsG0880044229.01.T01:CDS	35.0%
!!	TTGTTGGTAGCTCAAGGAAA+CGG	+	Chr8:37990807-37990826	MsG0880044229.01.T01:CDS	40.0%

Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	37990653	37991036	37990653	ID=MsG0880044229.01;Name=MsG0880044229.01
Chr8	mRNA	37990653	37991036	37990653	ID=MsG0880044229.01.T01;Parent=MsG0880044229.01;Name=MsG0880044229.01.T01;_AED=0.27;_eAED=0.27;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|127
Chr8	exon	37990653	37991036	37990653	ID=MsG0880044229.01.T01:exon:16772;Parent=MsG0880044229.01.T01
Chr8	CDS	37990653	37991036	37990653	ID=MsG0880044229.01.T01:cds;Parent=MsG0880044229.01.T01

Gene Sequence

>MsG0880044229.01.T01

ATGAAGAAAATCAATCTATTACTAAGAAAGTGCAAGAGCTTGTCAAAGCAATTAGGAAGATCATCATCATATAGTAGCTTAAGGTCAAAATCAAATAGAGAAGATTTATATGAAGGGCATGGTATGCAACAAGATGAAAATTGTGAAACTATATTTGTTGGTAGCTCAAGGAAACGGTATGTGATAAGTTCAAAGTATTTGAATCATCCTCTTCTTGAAGCTTTGATCAACAAGTCTAAGCAAAAGGGTAGTGATAATGAAAATGTTTTGGTTGTTAATTGTGAGGTTGTTCTCTTTGATCATTTATTATGGATGCTAGAAAATGCTGATCCTAAGTTTAGTTCTGAATCTTTAGAGGAATTGGCTGAACTTTATGTGTTCTAA

Protein sequence

>MsG0880044229.01.T01

MKKINLLLRKCKSLSKQLGRSSSYSSLRSKSNREDLYEGHGMQQDENCETIFVGSSRKRYVISSKYLNHPLLEALINKSKQKGSDNENVLVVNCEVVLFDHLLWMLENADPKFSSESLEELAELYVF*