AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180006024.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180006024.01.T01 MTR_1g112160 95.455 110 5 0 1 110 142 251 1.21e-75 224
MsG0180006024.01.T01 MTR_1g112150 68.868 106 31 2 1 105 191 295 6.94e-47 152
MsG0180006024.01.T01 MTR_2g021690 51.351 111 50 4 1 109 194 302 5.77e-26 98.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180006024.01.T01 AT1G55230 47.368 95 49 1 3 97 198 291 3.63e-26 99.0
MsG0180006024.01.T01 AT1G55240 47.368 95 50 0 3 97 198 292 5.79e-26 98.6
MsG0180006024.01.T01 AT5G19870 51.220 82 40 0 1 82 195 276 8.22e-26 97.4

Find 21 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTTGCCTGTGGTGATCTTT+TGG 0.244017 1:-98970611 None:intergenic
AGGTTTGTGGCTTATGTTCA+TGG 0.281409 1:+98970302 None:intergenic
CAATTCAGCTGGTTCTTGAT+TGG 0.309654 1:+98970450 MsG0180006024.01.T01:CDS
TGTGTCCTTCTACTTGATTA+TGG 0.364513 1:+98970488 MsG0180006024.01.T01:CDS
TTGATTATGGCTGTAAGTTA+TGG 0.394009 1:+98970501 MsG0180006024.01.T01:CDS
CAAGTTATTCAGATTTGCTC+TGG 0.427300 1:-98970633 None:intergenic
TACAGCCATAATCAAGTAGA+AGG 0.441076 1:-98970493 None:intergenic
GGTTTGTGGCTTATGTTCAT+GGG 0.442596 1:+98970303 None:intergenic
TGGTGAATCTTCAATTCAGC+TGG 0.455765 1:+98970439 MsG0180006024.01.T01:CDS
GGTTGCTTTACGAAACTTGA+GGG 0.479857 1:+98970360 MsG0180006024.01.T01:CDS
CAACCTTTCGGTTCAAAACC+AGG 0.497142 1:-98970342 None:intergenic
AGTTTCGTAAAGCAACCTTT+CGG 0.522864 1:-98970354 None:intergenic
GAGGAACATTATCGTGAAGA+TGG 0.535159 1:+98970558 MsG0180006024.01.T01:CDS
TGTTAAGTGCAGTGATGAAA+AGG 0.547706 1:+98970395 MsG0180006024.01.T01:CDS
TCTTCATCGTGCTGTAGCGT+TGG 0.552622 1:+98970419 MsG0180006024.01.T01:CDS
GTTTGTGGCTTATGTTCATG+GGG 0.570855 1:+98970304 None:intergenic
GATTTGCTCTGGCTTGCCTG+TGG 0.582887 1:-98970622 None:intergenic
GTATGTTTCACTGATGATGA+AGG 0.586326 1:+98970536 MsG0180006024.01.T01:CDS
CAATGCCAAAAGATCACCAC+AGG 0.593405 1:+98970606 MsG0180006024.01.T01:CDS
AGGTTGCTTTACGAAACTTG+AGG 0.630393 1:+98970359 MsG0180006024.01.T01:CDS
TGTTTCACTGATGATGAAGG+AGG 0.670252 1:+98970539 MsG0180006024.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TTGATTATGGCTGTAAGTTA+TGG + Chr1:98970501-98970520 MsG0180006024.01.T01:CDS 30.0%
AGTTTCGTAAAGCAACCTTT+CGG - Chr1:98970357-98970376 None:intergenic 35.0%
TACAGCCATAATCAAGTAGA+AGG - Chr1:98970496-98970515 None:intergenic 35.0%
TGTGTCCTTCTACTTGATTA+TGG + Chr1:98970488-98970507 MsG0180006024.01.T01:CDS 35.0%
TGTTAAGTGCAGTGATGAAA+AGG + Chr1:98970395-98970414 MsG0180006024.01.T01:CDS 35.0%
! GTATGTTTCACTGATGATGA+AGG + Chr1:98970536-98970555 MsG0180006024.01.T01:CDS 35.0%
GAGGAACATTATCGTGAAGA+TGG + Chr1:98970558-98970577 MsG0180006024.01.T01:CDS 40.0%
TGGTGAATCTTCAATTCAGC+TGG + Chr1:98970439-98970458 MsG0180006024.01.T01:CDS 40.0%
! AGGTTGCTTTACGAAACTTG+AGG + Chr1:98970359-98970378 MsG0180006024.01.T01:CDS 40.0%
! CAATTCAGCTGGTTCTTGAT+TGG + Chr1:98970450-98970469 MsG0180006024.01.T01:CDS 40.0%
! GGTTGCTTTACGAAACTTGA+GGG + Chr1:98970360-98970379 MsG0180006024.01.T01:CDS 40.0%
! TGTGGTGATCTTTTGGCATT+GGG - Chr1:98970607-98970626 None:intergenic 40.0%
! TGTTTCACTGATGATGAAGG+AGG + Chr1:98970539-98970558 MsG0180006024.01.T01:CDS 40.0%
CAACCTTTCGGTTCAAAACC+AGG - Chr1:98970345-98970364 None:intergenic 45.0%
CAATGCCAAAAGATCACCAC+AGG + Chr1:98970606-98970625 MsG0180006024.01.T01:CDS 45.0%
! CTGTGGTGATCTTTTGGCAT+TGG - Chr1:98970608-98970627 None:intergenic 45.0%
!!! ACACCTGGTTTTGAACCGAA+AGG + Chr1:98970339-98970358 MsG0180006024.01.T01:CDS 45.0%
TCTTCATCGTGCTGTAGCGT+TGG + Chr1:98970419-98970438 MsG0180006024.01.T01:CDS 50.0%
! GCTTGCCTGTGGTGATCTTT+TGG - Chr1:98970614-98970633 None:intergenic 50.0%
!! GGGCTCATGCTTTTTACACC+TGG + Chr1:98970324-98970343 MsG0180006024.01.T01:CDS 50.0%
! GATTTGCTCTGGCTTGCCTG+TGG - Chr1:98970625-98970644 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr1 gene 98970315 98970647 98970315 ID=MsG0180006024.01;Name=MsG0180006024.01
Chr1 mRNA 98970315 98970647 98970315 ID=MsG0180006024.01.T01;Parent=MsG0180006024.01;Name=MsG0180006024.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|110
Chr1 exon 98970315 98970647 98970315 ID=MsG0180006024.01.T01:exon:11007;Parent=MsG0180006024.01.T01
Chr1 CDS 98970315 98970647 98970315 ID=MsG0180006024.01.T01:cds;Parent=MsG0180006024.01.T01
Gene Sequence

>MsG0180006024.01.T01

ATGTTCATGGGGCTCATGCTTTTTACACCTGGTTTTGAACCGAAAGGTTGCTTTACGAAACTTGAGGGAGATCAATATGTTGTTAAGTGCAGTGATGAAAAGGCTCTTCATCGTGCTGTAGCGTTGGTGAATCTTCAATTCAGCTGGTTCTTGATTGGAGTCACTGTTTTTGCTGTGTCCTTCTACTTGATTATGGCTGTAAGTTATGGCGAAAAAGTAGAGTATGTTTCACTGATGATGAAGGAGGAACATTATCGTGAAGATGGCTTAAAAAATTTAGATGTTGAGTCCCAATGCCAAAAGATCACCACAGGCAAGCCAGAGCAAATCTGA

Protein sequence

>MsG0180006024.01.T01

MFMGLMLFTPGFEPKGCFTKLEGDQYVVKCSDEKALHRAVALVNLQFSWFLIGVTVFAVSFYLIMAVSYGEKVEYVSLMMKEEHYREDGLKNLDVESQCQKITTGKPEQI*