AlfalfaGEDB | MsG0880045128.01

M. sativa cultivar ZhongmuNo.1 / MsG0880045128.01

KEGG: -
GO: GO:0008146
PFAM: PF00685

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880045128.01.T01	RHN41447.1	46.545	275	70	4	2	199	38	312	4.37E-69	223
MsG0880045128.01.T02	PNX60102.1	74.731	186	47	0	16	201	63	248	2.15E-97	293
MsG0880045128.01.T03	PNX60102.1	74.731	186	47	0	8	193	63	248	1.95E-97	293
MsG0880045128.01.T04	XP_003628744.1	75.397	126	31	0	2	127	84	209	6.46E-62	198
MsG0880045128.01.T05	XP_039688440.1	70.408	98	29	0	1	98	27	124	6.79E-43	146
MsG0880045128.01.T06	XP_031273360.1	61.404	57	22	0	1	57	278	334	6.10E-17	82.8

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880045128.01.T01	Q8RUC1	36.364	253	86	4	6	199	37	273	3.38E-43	148
MsG0880045128.01.T02	Q9M1V2	52.174	184	88	0	18	201	140	323	2.71E-62	199
MsG0880045128.01.T03	Q9M1V2	52.174	184	88	0	10	193	140	323	1.91E-62	199
MsG0880045128.01.T04	Q9M1V2	54.762	126	57	0	2	127	198	323	5.02E-44	149
MsG0880045128.01.T05	Q9M1V2	53.061	98	46	0	1	98	226	323	4.65E-28	106
MsG0880045128.01.T06	Q9M1V2	52.632	57	27	0	1	57	267	323	9.63E-13	63.5

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880045128.01.T01	A0A396GRY2	46.545	275	70	4	2	199	38	312	2.09e-69	223
MsG0880045128.01.T02	A0A2K3K1H1	74.731	186	47	0	16	201	63	248	1.03e-97	293
MsG0880045128.01.T03	A0A2K3K1H1	74.731	186	47	0	8	193	63	248	9.33e-98	293
MsG0880045128.01.T04	G7LGX1	75.397	126	31	0	2	127	84	209	3.08e-62	198
MsG0880045128.01.T05	G7IXE9	72.449	98	27	0	1	98	236	333	2.92e-41	147
MsG0880045128.01.T06	A0A151S4L2	63.158	57	21	0	1	57	277	333	2.05e-16	80.5

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180004652.01	MsG0880045128.01	0.804601	1.997256e-49	1.034124e-46
MsG0280007505.01	MsG0880045128.01	0.814405	1.566501e-51	1.050243e-48
MsG0380017030.01	MsG0880045128.01	0.821216	4.542439e-53	3.673005e-50
MsG0380017901.01	MsG0880045128.01	0.802207	6.259804e-49	3.048242e-46
MsG0380018004.01	MsG0880045128.01	0.809867	1.530540e-50	9.088857e-48
MsG0480020810.01	MsG0880045128.01	0.804970	1.672836e-49	8.744614e-47
MsG0480021396.01	MsG0880045128.01	0.803598	3.230255e-49	1.630012e-46
MsG0580029696.01	MsG0880045128.01	0.800856	1.184172e-48	5.572869e-46
MsG0680030296.01	MsG0880045128.01	0.815516	8.884472e-52	6.138742e-49
MsG0680030613.01	MsG0880045128.01	0.800701	1.273850e-48	5.970864e-46

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880045128.01.T01	MTR_3g434790	49.268	205	88	3	2	199	39	234	6.58e-55	174
MsG0880045128.01.T01	MTR_0071s0010	70.833	120	35	0	80	199	214	333	4.15e-54	176
MsG0880045128.01.T01	MTR_0071s0010	73.404	94	25	0	2	95	37	130	9.93e-45	151
MsG0880045128.01.T01	MTR_3g046670	70.833	120	35	0	80	199	214	333	4.15e-54	176
MsG0880045128.01.T01	MTR_3g046670	73.404	94	25	0	2	95	37	130	9.93e-45	151
MsG0880045128.01.T01	MTR_8g066280	70.000	120	36	0	80	199	90	209	4.22e-54	172
MsG0880045128.01.T01	MTR_3g435130	67.500	120	39	0	80	199	215	334	3.06e-52	171
MsG0880045128.01.T01	MTR_3g435130	73.563	87	23	0	2	88	38	124	3.13e-39	137
MsG0880045128.01.T01	MTR_8g066210	65.000	120	42	0	80	199	199	318	1.76e-50	166
MsG0880045128.01.T01	MTR_8g066210	70.330	91	26	1	2	92	38	127	2.70e-38	134

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880045128.01.T01	AT2G27570	36.364	253	86	4	6	199	37	273	3.45e-44	148
MsG0880045128.01.T01	AT3G45070	50.000	120	60	0	80	199	204	323	2.40e-35	127
MsG0880045128.01.T01	AT3G45070	62.651	83	30	1	6	88	32	113	1.36e-27	106
MsG0880045128.01.T01	AT3G45070	50.000	120	60	0	80	199	209	328	2.89e-35	127
MsG0880045128.01.T01	AT3G45070	62.651	83	30	1	6	88	37	118	1.35e-27	106
MsG0880045128.01.T01	AT3G45070	50.000	120	60	0	80	199	236	355	3.49e-35	127
MsG0880045128.01.T01	AT3G45070	62.651	83	30	1	6	88	64	145	1.19e-27	107
MsG0880045128.01.T01	AT1G13420	52.252	111	53	0	87	197	219	329	1.56e-32	119
MsG0880045128.01.T01	AT1G13420	56.627	83	33	1	6	88	43	122	1.36e-23	95.9
MsG0880045128.01.T01	AT3G45080	47.934	121	62	1	80	199	209	329	7.78e-32	117

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Find 62 sgRNAs with CRISPR-Local

Find 77 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACTCCTTGAAGATCTTGTG+TGG	0.593904	8:-54608119	None:intergenic
AACTTGGAGACTAATAAGAC+TGG	0.514423	8:+54608804	MsG0880045128.01.T01:CDS
AAGAAGGGAACAAGAGCATG+AGG	0.605363	8:-54608304	None:intergenic
AAGACTGGGAAATTGCCTTC+TGG	0.423203	8:+54608819	MsG0880045128.01.T01:CDS
AAGAGAAATGGGCTAAACAT+GGG	0.620553	8:+54608937	MsG0880045128.01.T01:CDS
AAGTTAAGACCACATAGTTG+GGG	0.664111	8:+54608513	MsG0880045128.01.T01:intron
ACAAAAGAAGAAGAATCCAA+AGG	0.648101	8:+54608735	MsG0880045128.01.T01:CDS
ACAAGTTAAGACCACATAGT+TGG	0.537480	8:+54608511	MsG0880045128.01.T01:intron
ACACTATCACTGAAGAGAAA+TGG	0.382705	8:+54608925	MsG0880045128.01.T01:CDS
ACTTGGAGACTAATAAGACT+GGG	0.632456	8:+54608805	MsG0880045128.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACAAAAGAAGAAGAATCCAA+AGG	+	Chr8:54608735-54608754	MsG0880045128.01.T01:CDS	30.0%
CTACGATTATATCTGTATCA+TGG	-	Chr8:54608171-54608190	None:intergenic	30.0%
GAATGTATGGATATTTCTTG+CGG	-	Chr8:54608252-54608271	None:intergenic	30.0%
GTTGAGTCTTTGATAGATTT+CGG	-	Chr8:54608426-54608445	None:intergenic	30.0%
TATCAAAGACTCAACTTGTA+AGG	+	Chr8:54608431-54608450	MsG0880045128.01.T01:intron	30.0%
TCTCAGATGAAATGATTGAA+CGG	+	Chr8:54608898-54608917	MsG0880045128.01.T01:CDS	30.0%
TTTGCCAAAATCCTTGATAT+TGG	-	Chr8:54608099-54608118	None:intergenic	30.0%
AACTTGGAGACTAATAAGAC+TGG	+	Chr8:54608804-54608823	MsG0880045128.01.T01:CDS	35.0%
AAGTTAAGACCACATAGTTG+GGG	+	Chr8:54608513-54608532	MsG0880045128.01.T01:intron	35.0%
ACAAGTTAAGACCACATAGT+TGG	+	Chr8:54608511-54608530	MsG0880045128.01.T01:intron	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	54608076	54608971	54608076	ID=MsG0880045128.01;Name=MsG0880045128.01
Chr8	mRNA	54608076	54608971	54608076	ID=MsG0880045128.01.T01;Parent=MsG0880045128.01;Name=MsG0880045128.01.T01;_AED=0.49;_eAED=0.49;_QI=0\|0\|0\|1\|0\|0\|2\|0\|199
Chr8	exon	54608076	54608330	54608076	ID=MsG0880045128.01.T01:exon:23504;Parent=MsG0880045128.01.T01
Chr8	exon	54608627	54608971	54608627	ID=MsG0880045128.01.T01:exon:23505;Parent=MsG0880045128.01.T01
Chr8	CDS	54608076	54608330	54608076	ID=MsG0880045128.01.T01:cds;Parent=MsG0880045128.01.T01
Chr8	CDS	54608627	54608971	54608627	ID=MsG0880045128.01.T01:cds;Parent=MsG0880045128.01.T01
Chr8	mRNA	54608366	54608971	54608366	ID=MsG0880045128.01.T02;Parent=MsG0880045128.01;Name=MsG0880045128.01.T02;_AED=0.49;_eAED=0.49;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|201
Chr8	exon	54608366	54608971	54608366	ID=MsG0880045128.01.T02:exon:23506;Parent=MsG0880045128.01.T02
Chr8	CDS	54608366	54608971	54608366	ID=MsG0880045128.01.T02:cds;Parent=MsG0880045128.01.T02
Chr8	mRNA	54608390	54608971	54608390	ID=MsG0880045128.01.T03;Parent=MsG0880045128.01;Name=MsG0880045128.01.T03;_AED=0.49;_eAED=0.49;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|193
Chr8	exon	54608390	54608971	54608390	ID=MsG0880045128.01.T03:exon:23507;Parent=MsG0880045128.01.T03
Chr8	CDS	54608390	54608971	54608390	ID=MsG0880045128.01.T03:cds;Parent=MsG0880045128.01.T03
Chr8	mRNA	54608588	54608971	54608588	ID=MsG0880045128.01.T04;Parent=MsG0880045128.01;Name=MsG0880045128.01.T04;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|127
Chr8	exon	54608588	54608971	54608588	ID=MsG0880045128.01.T04:exon:23508;Parent=MsG0880045128.01.T04
Chr8	CDS	54608588	54608971	54608588	ID=MsG0880045128.01.T04:cds;Parent=MsG0880045128.01.T04
Chr8	mRNA	54608675	54608971	54608675	ID=MsG0880045128.01.T05;Parent=MsG0880045128.01;Name=MsG0880045128.01.T05;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|98
Chr8	exon	54608675	54608971	54608675	ID=MsG0880045128.01.T05:exon:23509;Parent=MsG0880045128.01.T05
Chr8	CDS	54608675	54608971	54608675	ID=MsG0880045128.01.T05:cds;Parent=MsG0880045128.01.T05
Chr8	mRNA	54608798	54608971	54608798	ID=MsG0880045128.01.T06;Parent=MsG0880045128.01;Name=MsG0880045128.01.T06;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|57
Chr8	exon	54608798	54608971	54608798	ID=MsG0880045128.01.T06:exon:23510;Parent=MsG0880045128.01.T06
Chr8	CDS	54608798	54608971	54608798	ID=MsG0880045128.01.T06:cds;Parent=MsG0880045128.01.T06

Gene Sequence

>MsG0880045128.01.T03

GCTCTTCTCTACACATCTTCTTATGAATCATTGCCGAAATCTATCAAAGACTCAACTTGTAAGGTGGTGTATATTTGTAGAGATCCAAAAGACACTTTTGTTTCACTTTTGCATTTTGTGAACAAGTTAAGACCACATAGTTGGGGAACACTTCCATTAGAAGAGGCATTCGAGAGTTTTTGTAGAGGAGTGAGTCCAATGGGACCCTTTTGGGAGCATGTATTAGGATATTGGCAGGAGAGTTTGGAAAAACCAAAGAAAGTTATGTTTTTGAGATATGAAGAAATGAAAATGAACCCTAATTTTTATTTGAAAAAGATTGCTAAGTTTTTGGAGTGTCCATTTACAAAAGAAGAAGAATCCAAAGGTGTGGTTGGTGATATATTAAACTTGTGTAGTTTTGAGAAGATGAGCAACTTGGAGACTAATAAGACTGGGAAATTGCCTTCTGGTGAACAAAATAATATTTTCTTTCGTCGAGGCCAAGTTGGAGATAGCAAAAATTTTCTCTCAGATGAAATGATTGAACGGTTGAACACTATCACTGAAGAGAAATGGGCTAAACATGGGTTGAGTTTTTAG

>MsG0880045128.01.T06

ATGAGCAACTTGGAGACTAATAAGACTGGGAAATTGCCTTCTGGTGAACAAAATAATATTTTCTTTCGTCGAGGCCAAGTTGGAGATAGCAAAAATTTTCTCTCAGATGAAATGATTGAACGGTTGAACACTATCACTGAAGAGAAATGGGCTAAACATGGGTTGAGTTTTTAG

>MsG0880045128.01.T05

ATGAAAATGAACCCTAATTTTTATTTGAAAAAGATTGCTAAGTTTTTGGAGTGTCCATTTACAAAAGAAGAAGAATCCAAAGGTGTGGTTGGTGATATATTAAACTTGTGTAGTTTTGAGAAGATGAGCAACTTGGAGACTAATAAGACTGGGAAATTGCCTTCTGGTGAACAAAATAATATTTTCTTTCGTCGAGGCCAAGTTGGAGATAGCAAAAATTTTCTCTCAGATGAAATGATTGAACGGTTGAACACTATCACTGAAGAGAAATGGGCTAAACATGGGTTGAGTTTTTAG

>MsG0880045128.01.T04

ATGGGACCCTTTTGGGAGCATGTATTAGGATATTGGCAGGAGAGTTTGGAAAAACCAAAGAAAGTTATGTTTTTGAGATATGAAGAAATGAAAATGAACCCTAATTTTTATTTGAAAAAGATTGCTAAGTTTTTGGAGTGTCCATTTACAAAAGAAGAAGAATCCAAAGGTGTGGTTGGTGATATATTAAACTTGTGTAGTTTTGAGAAGATGAGCAACTTGGAGACTAATAAGACTGGGAAATTGCCTTCTGGTGAACAAAATAATATTTTCTTTCGTCGAGGCCAAGTTGGAGATAGCAAAAATTTTCTCTCAGATGAAATGATTGAACGGTTGAACACTATCACTGAAGAGAAATGGGCTAAACATGGGTTGAGTTTTTAG

>MsG0880045128.01.T02

TCTAGATTCATTATCCCCTCCAAGGCTCTTCTCTACACATCTTCTTATGAATCATTGCCGAAATCTATCAAAGACTCAACTTGTAAGGTGGTGTATATTTGTAGAGATCCAAAAGACACTTTTGTTTCACTTTTGCATTTTGTGAACAAGTTAAGACCACATAGTTGGGGAACACTTCCATTAGAAGAGGCATTCGAGAGTTTTTGTAGAGGAGTGAGTCCAATGGGACCCTTTTGGGAGCATGTATTAGGATATTGGCAGGAGAGTTTGGAAAAACCAAAGAAAGTTATGTTTTTGAGATATGAAGAAATGAAAATGAACCCTAATTTTTATTTGAAAAAGATTGCTAAGTTTTTGGAGTGTCCATTTACAAAAGAAGAAGAATCCAAAGGTGTGGTTGGTGATATATTAAACTTGTGTAGTTTTGAGAAGATGAGCAACTTGGAGACTAATAAGACTGGGAAATTGCCTTCTGGTGAACAAAATAATATTTTCTTTCGTCGAGGCCAAGTTGGAGATAGCAAAAATTTTCTCTCAGATGAAATGATTGAACGGTTGAACACTATCACTGAAGAGAAATGGGCTAAACATGGGTTGAGTTTTTAG

>MsG0880045128.01.T01

ATGATTACACCCTACGTCCACCAATATCAAGGATTTTGGCAAACCACACAAGATCTTCAAGGAGTTTTATCTTGTCAAAAACACTTTCATGCCCATGATACAGATATAATCGTAGCTACCACTCCCAAATCAGGTACCACTTGGCTCAAAGCATTAACATTTGCCTTGCTCAACCGCAAGAAATATCCATACATTCATGATAACAATCATCCTTTGCTTACTAACAACCCTCATGCTCTTGTTCCCTTCTTGGAAGAGAGTTTGGAAAAACCAAAGAAAGTTATGTTTTTGAGATATGAAGAAATGAAAATGAACCCTAATTTTTATTTGAAAAAGATTGCTAAGTTTTTGGAGTGTCCATTTACAAAAGAAGAAGAATCCAAAGGTGTGGTTGGTGATATATTAAACTTGTGTAGTTTTGAGAAGATGAGCAACTTGGAGACTAATAAGACTGGGAAATTGCCTTCTGGTGAACAAAATAATATTTTCTTTCGTCGAGGCCAAGTTGGAGATAGCAAAAATTTTCTCTCAGATGAAATGATTGAACGGTTGAACACTATCACTGAAGAGAAATGGGCTAAACATGGGTTGAGTTTTTAG

Protein sequence

>MsG0880045128.01.T03

ALLYTSSYESLPKSIKDSTCKVVYICRDPKDTFVSLLHFVNKLRPHSWGTLPLEEAFESFCRGVSPMGPFWEHVLGYWQESLEKPKKVMFLRYEEMKMNPNFYLKKIAKFLECPFTKEEESKGVVGDILNLCSFEKMSNLETNKTGKLPSGEQNNIFFRRGQVGDSKNFLSDEMIERLNTITEEKWAKHGLSF*

>MsG0880045128.01.T06

MSNLETNKTGKLPSGEQNNIFFRRGQVGDSKNFLSDEMIERLNTITEEKWAKHGLSF*

>MsG0880045128.01.T05

MKMNPNFYLKKIAKFLECPFTKEEESKGVVGDILNLCSFEKMSNLETNKTGKLPSGEQNNIFFRRGQVGDSKNFLSDEMIERLNTITEEKWAKHGLSF*

>MsG0880045128.01.T04

MGPFWEHVLGYWQESLEKPKKVMFLRYEEMKMNPNFYLKKIAKFLECPFTKEEESKGVVGDILNLCSFEKMSNLETNKTGKLPSGEQNNIFFRRGQVGDSKNFLSDEMIERLNTITEEKWAKHGLSF*

>MsG0880045128.01.T02

SRFIIPSKALLYTSSYESLPKSIKDSTCKVVYICRDPKDTFVSLLHFVNKLRPHSWGTLPLEEAFESFCRGVSPMGPFWEHVLGYWQESLEKPKKVMFLRYEEMKMNPNFYLKKIAKFLECPFTKEEESKGVVGDILNLCSFEKMSNLETNKTGKLPSGEQNNIFFRRGQVGDSKNFLSDEMIERLNTITEEKWAKHGLSF*

>MsG0880045128.01.T01

MITPYVHQYQGFWQTTQDLQGVLSCQKHFHAHDTDIIVATTPKSGTTWLKALTFALLNRKKYPYIHDNNHPLLTNNPHALVPFLEESLEKPKKVMFLRYEEMKMNPNFYLKKIAKFLECPFTKEEESKGVVGDILNLCSFEKMSNLETNKTGKLPSGEQNNIFFRRGQVGDSKNFLSDEMIERLNTITEEKWAKHGLSF*