Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480020810.01.T01 | XP_003606401.1 | 94.33 | 388 | 7 | 2 | 1 | 375 | 1 | 386 | 0 | 758 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480020810.01.T01 | G7JPE3 | 94.330 | 388 | 7 | 2 | 1 | 375 | 1 | 386 | 0.0 | 758 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047985.01 | MsG0480020810.01 | 0.807390 | 5.174836e-50 | 2.878742e-47 |
| MsG0080048134.01 | MsG0480020810.01 | 0.808236 | 3.419573e-50 | 1.944946e-47 |
| MsG0080049008.01 | MsG0480020810.01 | 0.828511 | 8.633153e-55 | 8.599373e-52 |
| MsG0180000028.01 | MsG0480020810.01 | 0.819882 | 9.196983e-53 | 7.165604e-50 |
| MsG0180000029.01 | MsG0480020810.01 | 0.812054 | 5.142506e-51 | 3.235762e-48 |
| MsG0180000495.01 | MsG0480020810.01 | 0.817718 | 2.851802e-52 | 2.092846e-49 |
| MsG0180000497.01 | MsG0480020810.01 | 0.837980 | 3.774010e-57 | 4.985147e-54 |
| MsG0180001004.01 | MsG0480020810.01 | 0.816997 | 4.143021e-52 | 2.981185e-49 |
| MsG0180001029.01 | MsG0480020810.01 | 0.819451 | 1.153593e-52 | 8.880644e-50 |
| MsG0180001499.01 | MsG0480020810.01 | 0.801043 | 1.084722e-48 | 5.129189e-46 |
| MsG0180003320.01 | MsG0480020810.01 | 0.829845 | 4.097890e-55 | 4.244968e-52 |
| MsG0180003405.01 | MsG0480020810.01 | -0.801799 | 7.593024e-49 | 3.659398e-46 |
| MsG0180003505.01 | MsG0480020810.01 | 0.828503 | 8.670994e-55 | 8.635079e-52 |
| MsG0180004467.01 | MsG0480020810.01 | 0.811177 | 7.975450e-51 | 4.902972e-48 |
| MsG0180004549.01 | MsG0480020810.01 | 0.809492 | 1.842166e-50 | 1.083131e-47 |
| MsG0180004610.01 | MsG0480020810.01 | 0.815110 | 1.093265e-51 | 7.469724e-49 |
| MsG0180004948.01 | MsG0480020810.01 | 0.800777 | 1.229280e-48 | 5.773130e-46 |
| MsG0280011281.01 | MsG0480020810.01 | -0.802004 | 6.891809e-49 | 3.338753e-46 |
| MsG0280011393.01 | MsG0480020810.01 | 0.811578 | 6.527157e-51 | 4.055251e-48 |
| MsG0380011686.01 | MsG0480020810.01 | 0.816792 | 4.606156e-52 | 3.295601e-49 |
| MsG0380011701.01 | MsG0480020810.01 | 0.819740 | 9.908845e-53 | 7.689855e-50 |
| MsG0380012900.01 | MsG0480020810.01 | 0.821982 | 3.022398e-53 | 2.496904e-50 |
| MsG0380013843.01 | MsG0480020810.01 | 0.827914 | 1.202032e-54 | 1.176664e-51 |
| MsG0480019146.01 | MsG0480020810.01 | 0.828053 | 1.113317e-54 | 1.094289e-51 |
| MsG0480019171.01 | MsG0480020810.01 | 0.820031 | 8.502768e-53 | 6.652782e-50 |
| MsG0480019663.01 | MsG0480020810.01 | 0.840892 | 6.614419e-58 | 9.555640e-55 |
| MsG0480019818.01 | MsG0480020810.01 | 0.803801 | 2.930443e-49 | 1.486429e-46 |
| MsG0480019842.01 | MsG0480020810.01 | 0.813700 | 2.241034e-51 | 1.473957e-48 |
| MsG0480020118.01 | MsG0480020810.01 | 0.839723 | 1.335878e-57 | 1.860936e-54 |
| MsG0480020810.01 | MsG0480020811.01 | 0.822298 | 2.552710e-53 | 2.128169e-50 |
| MsG0480020810.01 | MsG0480020904.01 | 0.819001 | 1.460338e-52 | 1.110372e-49 |
| MsG0480020810.01 | MsG0480021133.01 | 0.800298 | 1.539134e-48 | 7.141402e-46 |
| MsG0480020810.01 | MsG0480022840.01 | 0.804368 | 2.233981e-49 | 1.149796e-46 |
| MsG0480020810.01 | MsG0580024087.01 | 0.800690 | 1.280552e-48 | 6.000746e-46 |
| MsG0480020810.01 | MsG0580024412.01 | 0.802371 | 5.790337e-49 | 2.831742e-46 |
| MsG0480020810.01 | MsG0580024591.01 | 0.801504 | 8.728355e-49 | 4.175328e-46 |
| MsG0480020810.01 | MsG0580024980.01 | 0.804955 | 1.684989e-49 | 8.804961e-47 |
| MsG0480020810.01 | MsG0580025432.01 | 0.809748 | 1.623237e-50 | 9.609883e-48 |
| MsG0480020810.01 | MsG0580025633.01 | 0.816585 | 5.125688e-52 | 3.646583e-49 |
| MsG0480020810.01 | MsG0580026180.01 | 0.800055 | 1.724199e-48 | 7.950747e-46 |
| MsG0480020810.01 | MsG0580026521.01 | 0.835230 | 1.893527e-56 | 2.301346e-53 |
| MsG0480020810.01 | MsG0580028302.01 | 0.814346 | 1.614693e-51 | 1.080756e-48 |
| MsG0480020810.01 | MsG0680030296.01 | 0.819666 | 1.030471e-52 | 7.980178e-50 |
| MsG0480020810.01 | MsG0680030613.01 | 0.832334 | 1.003316e-55 | 1.117820e-52 |
| MsG0480020810.01 | MsG0680033367.01 | 0.806327 | 8.680562e-50 | 4.698310e-47 |
| MsG0480020810.01 | MsG0680034238.01 | 0.809935 | 1.479909e-50 | 8.804002e-48 |
| MsG0480020810.01 | MsG0680034418.01 | 0.826213 | 3.069474e-54 | 2.860235e-51 |
| MsG0480020810.01 | MsG0780036839.01 | 0.827740 | 1.324220e-54 | 1.289616e-51 |
| MsG0480020810.01 | MsG0780037998.01 | 0.816449 | 5.498202e-52 | 3.896827e-49 |
| MsG0480020810.01 | MsG0780038307.01 | 0.804356 | 2.246697e-49 | 1.155983e-46 |
| MsG0480020810.01 | MsG0780038519.01 | 0.808564 | 2.910679e-50 | 1.669729e-47 |
| MsG0480020810.01 | MsG0780038657.01 | 0.844534 | 7.123200e-59 | 1.153501e-55 |
| MsG0480020810.01 | MsG0780039373.01 | 0.811140 | 8.123417e-51 | 4.989120e-48 |
| MsG0480020810.01 | MsG0780039619.01 | 0.817279 | 3.580591e-52 | 2.596100e-49 |
| MsG0480020810.01 | MsG0780039883.01 | 0.819426 | 1.168534e-52 | 8.989330e-50 |
| MsG0480020810.01 | MsG0780040560.01 | 0.824062 | 9.891159e-54 | 8.667713e-51 |
| MsG0480020810.01 | MsG0780040831.01 | 0.816944 | 4.258603e-52 | 3.059939e-49 |
| MsG0480020810.01 | MsG0780041036.01 | 0.818175 | 2.248280e-52 | 1.670752e-49 |
| MsG0480020810.01 | MsG0780041180.01 | 0.818425 | 1.973802e-52 | 1.477025e-49 |
| MsG0480020810.01 | MsG0780041248.01 | 0.823813 | 1.131910e-53 | 9.850717e-51 |
| MsG0480020810.01 | MsG0780041397.01 | 0.807163 | 5.780927e-50 | 3.196911e-47 |
| MsG0480020810.01 | MsG0880041977.01 | 0.800923 | 1.147502e-48 | 5.409437e-46 |
| MsG0480020810.01 | MsG0880042262.01 | 0.828658 | 7.953929e-55 | 7.956744e-52 |
| MsG0480020810.01 | MsG0880042591.01 | 0.822657 | 2.106939e-53 | 1.774612e-50 |
| MsG0480020810.01 | MsG0880043212.01 | 0.800011 | 1.760028e-48 | 8.106840e-46 |
| MsG0480020810.01 | MsG0880043495.01 | 0.803497 | 3.388501e-49 | 1.705444e-46 |
| MsG0480020810.01 | MsG0880043550.01 | 0.808879 | 2.492776e-50 | 1.441817e-47 |
| MsG0480020810.01 | MsG0880043882.01 | 0.829088 | 6.260907e-55 | 6.342140e-52 |
| MsG0480020810.01 | MsG0880043998.01 | 0.816094 | 6.601601e-52 | 4.634134e-49 |
| MsG0480020810.01 | MsG0880044712.01 | 0.814277 | 1.672065e-51 | 1.117074e-48 |
| MsG0480020810.01 | MsG0880045128.01 | 0.804970 | 1.672836e-49 | 8.744614e-47 |
| MsG0480020810.01 | MsG0880045130.01 | 0.800844 | 1.190830e-48 | 5.602422e-46 |
| MsG0480020810.01 | MsG0880045854.01 | 0.815607 | 8.477185e-52 | 5.872164e-49 |
| MsG0480020810.01 | MsG0880045949.01 | 0.802028 | 6.811940e-49 | 3.302145e-46 |
| MsG0480020810.01 | MsG0880046870.01 | 0.811594 | 6.473984e-51 | 4.023837e-48 |
| MsG0480020810.01 | MsG0880046932.01 | 0.815323 | 9.803790e-52 | 6.737898e-49 |
| MsG0480020810.01 | MsG0880047057.01 | 0.822285 | 2.570763e-53 | 2.142468e-50 |
| MsG0380015711.01 | MsG0480020810.01 | 0.809089 | 2.247949e-50 | 1.307610e-47 |
| MsG0380016120.01 | MsG0480020810.01 | 0.801132 | 1.040202e-48 | 4.929659e-46 |
| MsG0380016372.01 | MsG0480020810.01 | 0.810794 | 9.655737e-51 | 5.875993e-48 |
| MsG0380016485.01 | MsG0480020810.01 | 0.851116 | 1.094108e-60 | 2.191476e-57 |
| MsG0380016490.01 | MsG0480020810.01 | 0.822472 | 2.326553e-53 | 1.949224e-50 |
| MsG0380017030.01 | MsG0480020810.01 | 0.806487 | 8.033636e-50 | 4.365840e-47 |
| MsG0380017168.01 | MsG0480020810.01 | 0.826762 | 2.270457e-54 | 2.148996e-51 |
| MsG0380017901.01 | MsG0480020810.01 | 0.853595 | 2.154636e-61 | 4.680348e-58 |
| MsG0480018362.01 | MsG0480020810.01 | 0.818320 | 2.084697e-52 | 1.555386e-49 |
| MsG0280006546.01 | MsG0480020810.01 | 0.806184 | 9.304434e-50 | 5.017774e-47 |
| MsG0280006559.01 | MsG0480020810.01 | 0.801147 | 1.032946e-48 | 4.896966e-46 |
| MsG0280006579.01 | MsG0480020810.01 | 0.848718 | 5.127265e-60 | 9.495819e-57 |
| MsG0280007054.01 | MsG0480020810.01 | 0.809524 | 1.813715e-50 | 1.067311e-47 |
| MsG0280007199.01 | MsG0480020810.01 | 0.802813 | 4.693818e-49 | 2.321523e-46 |
| MsG0280007731.01 | MsG0480020810.01 | 0.807428 | 5.080193e-50 | 2.828850e-47 |
| MsG0280007937.01 | MsG0480020810.01 | 0.807760 | 4.319094e-50 | 2.425860e-47 |
| MsG0280009232.01 | MsG0480020810.01 | 0.813032 | 3.142712e-51 | 2.029788e-48 |
| MsG0280009432.01 | MsG0480020810.01 | 0.810486 | 1.125686e-50 | 6.793924e-48 |
| MsG0280009526.01 | MsG0480020810.01 | 0.811736 | 6.031285e-51 | 3.762925e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480020810.01.T01 | MTR_4g059630 | 94.330 | 388 | 7 | 2 | 1 | 375 | 1 | 386 | 0.0 | 758 |
| MsG0480020810.01.T01 | MTR_4g059670 | 89.869 | 306 | 30 | 1 | 70 | 375 | 91 | 395 | 0.0 | 566 |
| MsG0480020810.01.T01 | MTR_2g080260 | 47.018 | 285 | 145 | 4 | 94 | 375 | 41 | 322 | 1.46e-96 | 291 |
| MsG0480020810.01.T01 | MTR_2g080230 | 46.667 | 285 | 146 | 4 | 94 | 375 | 41 | 322 | 8.92e-94 | 284 |
| MsG0480020810.01.T01 | MTR_8g464300 | 47.037 | 270 | 139 | 3 | 94 | 362 | 41 | 307 | 2.25e-88 | 270 |
| MsG0480020810.01.T01 | MTR_2g030835 | 43.774 | 265 | 138 | 4 | 103 | 362 | 36 | 294 | 7.29e-76 | 238 |
| MsG0480020810.01.T01 | MTR_2g021580 | 43.019 | 265 | 140 | 4 | 103 | 362 | 37 | 295 | 3.67e-75 | 236 |
| MsG0480020810.01.T01 | MTR_2g030825 | 42.379 | 269 | 135 | 4 | 94 | 362 | 311 | 559 | 3.50e-72 | 236 |
| MsG0480020810.01.T01 | MTR_2g030845 | 42.007 | 269 | 136 | 4 | 94 | 362 | 312 | 560 | 8.47e-71 | 232 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480020810.01.T01 | AT5G60520 | 68.874 | 302 | 94 | 0 | 62 | 363 | 27 | 328 | 1.52e-155 | 441 |
| MsG0480020810.01.T01 | AT5G60530 | 63.323 | 319 | 116 | 1 | 46 | 363 | 111 | 429 | 8.04e-155 | 443 |
| MsG0480020810.01.T01 | AT4G27400 | 42.808 | 292 | 160 | 5 | 85 | 372 | 28 | 316 | 2.75e-90 | 275 |
| MsG0480020810.01.T01 | AT5G54370 | 42.500 | 280 | 154 | 4 | 94 | 372 | 36 | 309 | 6.61e-86 | 264 |
| MsG0480020810.01.T01 | AT1G54890 | 44.086 | 279 | 149 | 5 | 94 | 368 | 42 | 317 | 1.48e-85 | 263 |
| MsG0480020810.01.T01 | AT3G19430 | 42.456 | 285 | 145 | 6 | 81 | 362 | 258 | 526 | 4.45e-75 | 243 |
| MsG0480020810.01.T01 | AT3G19430 | 42.456 | 285 | 145 | 6 | 81 | 362 | 281 | 549 | 5.28e-75 | 243 |
Find 92 sgRNAs with CRISPR-Local
Find 121 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTGTTCTTCTTTGGCTTCC+TGG | 0.127198 | 4:-48713653 | None:intergenic |
| GAATTCGTCGTCTGAAACTA+TGG | 0.170329 | 4:-48714426 | None:intergenic |
| TTGACTCTCTTACAGTAAAA+TGG | 0.170606 | 4:+48714588 | MsG0480020810.01.T01:CDS |
| TTATCTTTCTTGTTCTTCTT+TGG | 0.195002 | 4:-48713661 | None:intergenic |
| TAATTTGATGATTCCTCTTT+TGG | 0.202224 | 4:-48713529 | None:intergenic |
| AGTGTTAGAGTCACAGTTTC+TGG | 0.225553 | 4:+48714710 | MsG0480020810.01.T01:CDS |
| GTTTATCTTACTTCTCTCTA+TGG | 0.333386 | 4:+48713369 | MsG0480020810.01.T01:CDS |
| TTGAAAAGAGAGATGGAGTT+TGG | 0.337740 | 4:-48714961 | None:intergenic |
| CAATGAAGTGTGCGTTGATT+TGG | 0.340778 | 4:-48714448 | None:intergenic |
| AGTTTCTGGTTTGCTTGAAA+TGG | 0.354070 | 4:+48714724 | MsG0480020810.01.T01:CDS |
| TATGGCGAAGTTTCCTCCTT+TGG | 0.367101 | 4:-48714408 | None:intergenic |
| ATTCCATTGAGTTACTCTAT+TGG | 0.378851 | 4:-48714558 | None:intergenic |
| TTGTCTTCTCCTCCCATCAT+TGG | 0.383781 | 4:-48714935 | None:intergenic |
| CGTTACCATTACCATTTCCT+TGG | 0.391236 | 4:-48713411 | None:intergenic |
| AATCCTACAGATTCTATTGA+AGG | 0.405188 | 4:+48714851 | MsG0480020810.01.T01:CDS |
| GACATAAGGGTAAGACCTAT+TGG | 0.405265 | 4:+48714746 | MsG0480020810.01.T01:CDS |
| AAGAAGAACAAGAAAGATAA+AGG | 0.413234 | 4:+48713664 | MsG0480020810.01.T01:CDS |
| CTTCATTGGTACTCGGCCTC+AGG | 0.419986 | 4:+48714463 | MsG0480020810.01.T01:CDS |
| GAAGGCAAGGAGAAAGGAAA+AGG | 0.426860 | 4:+48713469 | MsG0480020810.01.T01:CDS |
| GTTTGCTTGAAATGGACATA+AGG | 0.432171 | 4:+48714732 | MsG0480020810.01.T01:CDS |
| CTTGGCTAGTGGAAGGCCTC+TGG | 0.435290 | 4:-48715006 | None:intergenic |
| CAAATCAACGCACACTTCAT+TGG | 0.436180 | 4:+48714449 | MsG0480020810.01.T01:CDS |
| TGCTACGATCCTCGCTTTGT+TGG | 0.444194 | 4:+48714353 | MsG0480020810.01.T01:CDS |
| CCTCGCTTTGTTGGTGGAGA+CGG | 0.444632 | 4:+48714362 | MsG0480020810.01.T01:CDS |
| AGGAATTGCTATGCCAATGA+TGG | 0.444976 | 4:+48714922 | MsG0480020810.01.T01:CDS |
| GGTAAGACCTATTGGAGAAA+AGG | 0.448306 | 4:+48714754 | MsG0480020810.01.T01:CDS |
| CGGTAACAATGGAAATGGTA+AGG | 0.455538 | 4:+48713432 | MsG0480020810.01.T01:CDS |
| CAACAACATGCAAACTCTGC+AGG | 0.455560 | 4:+48714981 | MsG0480020810.01.T01:CDS |
| AAGGAAGAAGGCAAGGAGAA+AGG | 0.456765 | 4:+48713463 | MsG0480020810.01.T01:CDS |
| TTTGCTTGAAATGGACATAA+GGG | 0.458437 | 4:+48714733 | MsG0480020810.01.T01:CDS |
| ATTGCTCAAGGCCAAGGAAA+TGG | 0.467146 | 4:+48713400 | MsG0480020810.01.T01:CDS |
| GGTAACGGTAACAATGGAAA+TGG | 0.468560 | 4:+48713427 | MsG0480020810.01.T01:CDS |
| CTACCGATGATGACGCTGAA+TGG | 0.469881 | 4:+48714633 | MsG0480020810.01.T01:CDS |
| GAAAGGAAAAGGAAAAGAGA+AGG | 0.473332 | 4:+48713480 | MsG0480020810.01.T01:CDS |
| CAGTTGCAAACTTTGCCATC+AGG | 0.477219 | 4:+48713556 | MsG0480020810.01.T01:CDS |
| GCAAACTTTGCCATCAGGAC+AGG | 0.479019 | 4:+48713561 | MsG0480020810.01.T01:CDS |
| TACGATCCTCGCTTTGTTGG+TGG | 0.482458 | 4:+48714356 | MsG0480020810.01.T01:CDS |
| AGTTTCCTCCTTTGGCTCCA+TGG | 0.483265 | 4:-48714400 | None:intergenic |
| TGACTCTCTTACAGTAAAAT+GGG | 0.484783 | 4:+48714589 | MsG0480020810.01.T01:CDS |
| AATATTGAGCAATGACTCCT+TGG | 0.485386 | 4:-48715024 | None:intergenic |
| CATGTTGTTGAAAAGAGAGA+TGG | 0.491460 | 4:-48714968 | None:intergenic |
| AGAGCAAACTGTGATGGTTA+CGG | 0.511380 | 4:+48714323 | MsG0480020810.01.T01:CDS |
| CCAAGATGGCAGGAGGAAAG+TGG | 0.514698 | 4:+48713329 | None:intergenic |
| AGAGAGTTCACCAAGATGGC+AGG | 0.515343 | 4:+48713319 | None:intergenic |
| GACGCTGAATGGAAGACTAA+TGG | 0.526534 | 4:+48714644 | MsG0480020810.01.T01:CDS |
| AAATGGTAAGGGAAATGATA+AGG | 0.526665 | 4:+48713444 | MsG0480020810.01.T01:CDS |
| TTGACAGGACTAACATAACT+TGG | 0.528654 | 4:-48714896 | None:intergenic |
| TTCTACTTCCATGGAGCCAA+AGG | 0.530261 | 4:+48714392 | MsG0480020810.01.T01:CDS |
| AGGAGGAAAGTGGAGCATCT+TGG | 0.533383 | 4:+48713339 | MsG0480020810.01.T01:CDS |
| ATAGCAATTCCTCTCTTGAC+AGG | 0.533897 | 4:-48714911 | None:intergenic |
| GAAGGACTCGCGACTACACA+TGG | 0.541845 | 4:+48714486 | MsG0480020810.01.T01:CDS |
| GCTGCCATTGCTCAAGGCCA+AGG | 0.542712 | 4:+48713394 | MsG0480020810.01.T01:CDS |
| CAAGGCCAAGGAAATGGTAA+TGG | 0.543693 | 4:+48713406 | MsG0480020810.01.T01:CDS |
| AAAGAAGAAGGCTCCAAAAG+AGG | 0.546050 | 4:+48713516 | MsG0480020810.01.T01:CDS |
| AAGGGAAATGATAAGGAAGA+AGG | 0.548679 | 4:+48713451 | MsG0480020810.01.T01:CDS |
| AAATGATAAGGAAGAAGGCA+AGG | 0.550988 | 4:+48713456 | MsG0480020810.01.T01:CDS |
| TAGCCAATAGAGTAACTCAA+TGG | 0.551874 | 4:+48714555 | MsG0480020810.01.T01:CDS |
| AGCGTCATCATCGGTAGGAA+TGG | 0.553954 | 4:-48714627 | None:intergenic |
| AATGCTCGCTCCTGTCCTGA+TGG | 0.556099 | 4:-48713571 | None:intergenic |
| GATGAACTTACACTTGCAAG+TGG | 0.557056 | 4:-48713719 | None:intergenic |
| ATGGAAGCTGCCATTGCTCA+AGG | 0.563232 | 4:+48713388 | MsG0480020810.01.T01:CDS |
| AGCAATGACTCCTTGGCTAG+TGG | 0.564443 | 4:-48715017 | None:intergenic |
| ATTTCCTTGGCCTTGAGCAA+TGG | 0.568400 | 4:-48713398 | None:intergenic |
| GGTGTGATGTTCTACTTCCA+TGG | 0.570969 | 4:+48714383 | MsG0480020810.01.T01:CDS |
| GGTAATGGTAACGGTAACAA+TGG | 0.571925 | 4:+48713421 | MsG0480020810.01.T01:CDS |
| TTGGTACTCGGCCTCAGGGA+AGG | 0.575908 | 4:+48714468 | MsG0480020810.01.T01:CDS |
| CTTCCATTCAGCGTCATCAT+CGG | 0.576076 | 4:-48714636 | None:intergenic |
| GCAAACTCTGCAGGTTCCAG+AGG | 0.576301 | 4:+48714990 | MsG0480020810.01.T01:CDS |
| GGTAACAATGGAAATGGTAA+GGG | 0.582315 | 4:+48713433 | MsG0480020810.01.T01:CDS |
| TGATGAAAGAGTAGTAGTTG+TGG | 0.583327 | 4:+48714667 | MsG0480020810.01.T01:CDS |
| GGAATTGCTATGCCAATGAT+GGG | 0.584815 | 4:+48714923 | MsG0480020810.01.T01:CDS |
| GTCCAGCTGAGTGCAAAACC+AGG | 0.585455 | 4:+48713635 | MsG0480020810.01.T01:CDS |
| GTTAGAAGAGCAAACTGTGA+TGG | 0.591528 | 4:+48714317 | MsG0480020810.01.T01:intron |
| ACTCCTTCAATAGAATCTGT+AGG | 0.597663 | 4:-48714854 | None:intergenic |
| ACGCACACTTCATTGGTACT+CGG | 0.600159 | 4:+48714456 | MsG0480020810.01.T01:CDS |
| CAAGGAAATGGTAATGGTAA+CGG | 0.600503 | 4:+48713412 | MsG0480020810.01.T01:CDS |
| CAGAGGCCTTCCACTAGCCA+AGG | 0.603668 | 4:+48715007 | MsG0480020810.01.T01:CDS |
| TGAGCAAAAGCATCATCTGA+TGG | 0.608641 | 4:-48714803 | None:intergenic |
| TTCATTGGTACTCGGCCTCA+GGG | 0.609556 | 4:+48714464 | MsG0480020810.01.T01:CDS |
| ATGACTCCTTGGCTAGTGGA+AGG | 0.614412 | 4:-48715013 | None:intergenic |
| TACTTCCATGGAGCCAAAGG+AGG | 0.618849 | 4:+48714395 | MsG0480020810.01.T01:CDS |
| AGGAAAGTGGAGCATCTTGG+TGG | 0.627956 | 4:+48713342 | MsG0480020810.01.T01:CDS |
| TATTGGAGAAAAGGAAAACA+AGG | 0.629127 | 4:+48714763 | MsG0480020810.01.T01:CDS |
| CATTCAGCGTCATCATCGGT+AGG | 0.633170 | 4:-48714632 | None:intergenic |
| TATGTTAGTCCTGTCAAGAG+AGG | 0.634498 | 4:+48714902 | MsG0480020810.01.T01:CDS |
| GAGTTCACCAAGATGGCAGG+AGG | 0.649508 | 4:+48713322 | None:intergenic |
| TCTCTTACAGTAAAATGGGA+CGG | 0.652194 | 4:+48714593 | MsG0480020810.01.T01:CDS |
| CCGTCTCCACCAACAAAGCG+AGG | 0.670633 | 4:-48714362 | None:intergenic |
| AGAGAGAAGTAAGATAAACA+AGG | 0.672417 | 4:-48713365 | None:intergenic |
| ATTGCTATGCCAATGATGGG+AGG | 0.672930 | 4:+48714926 | MsG0480020810.01.T01:CDS |
| AAGGACTCGCGACTACACAT+GGG | 0.715742 | 4:+48714487 | MsG0480020810.01.T01:CDS |
| TAGTCGCGAGTCCTTCCCTG+AGG | 0.736450 | 4:-48714479 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTGGTATAATATTCAATT+TGG | + | Chr4:48713759-48713778 | MsG0480020810.01.T01:intron | 15.0% |
| !! | TAATTTAATTAGAGAATGAG+AGG | + | Chr4:48714041-48714060 | MsG0480020810.01.T01:intron | 20.0% |
| !! | TGCCTAAATTAATTACATTT+GGG | - | Chr4:48714082-48714101 | None:intergenic | 20.0% |
| !! | TTGCATTCAAAATATAATGT+TGG | - | Chr4:48713855-48713874 | None:intergenic | 20.0% |
| !!! | TTTATACTCTTCTATACTTT+TGG | + | Chr4:48713742-48713761 | MsG0480020810.01.T01:intron | 20.0% |
| ! | AAGAAGAACAAGAAAGATAA+AGG | + | Chr4:48713664-48713683 | MsG0480020810.01.T01:CDS | 25.0% |
| ! | ACCCCAAATGTAATTAATTT+AGG | + | Chr4:48714077-48714096 | MsG0480020810.01.T01:intron | 25.0% |
| ! | ATGAACTATTTGACTAAGTT+CGG | + | Chr4:48713817-48713836 | MsG0480020810.01.T01:intron | 25.0% |
| ! | GCCTAAATTAATTACATTTG+GGG | - | Chr4:48714081-48714100 | None:intergenic | 25.0% |
| ! | GTGCCTAAATTAATTACATT+TGG | - | Chr4:48714083-48714102 | None:intergenic | 25.0% |
| ! | TATTGTTATGTGTGCTTAAA+TGG | + | Chr4:48714176-48714195 | MsG0480020810.01.T01:intron | 25.0% |
| ! | TTATCTTTCTTGTTCTTCTT+TGG | - | Chr4:48713664-48713683 | None:intergenic | 25.0% |
| ! | TTATTACCATCAATCATTCT+CGG | - | Chr4:48714237-48714256 | None:intergenic | 25.0% |
| ! | TTCCTTACTATAGTAAATTC+AGG | + | Chr4:48713939-48713958 | MsG0480020810.01.T01:intron | 25.0% |
| !! | AAGAACAAAGAAGCATAAAA+TGG | - | Chr4:48714007-48714026 | None:intergenic | 25.0% |
| !!! | ATTGTACCTTTTTTAAGTTG+TGG | - | Chr4:48714110-48714129 | None:intergenic | 25.0% |
| !!! | TAATTTGATGATTCCTCTTT+TGG | - | Chr4:48713532-48713551 | None:intergenic | 25.0% |
| !!! | TTGTACCTTTTTTAAGTTGT+GGG | - | Chr4:48714109-48714128 | None:intergenic | 25.0% |
| AAATGGTAAGGGAAATGATA+AGG | + | Chr4:48713444-48713463 | MsG0480020810.01.T01:CDS | 30.0% | |
| AATCCTACAGATTCTATTGA+AGG | + | Chr4:48714851-48714870 | MsG0480020810.01.T01:CDS | 30.0% | |
| AGAGAGAAGTAAGATAAACA+AGG | - | Chr4:48713368-48713387 | None:intergenic | 30.0% | |
| ATTCCATTGAGTTACTCTAT+TGG | - | Chr4:48714561-48714580 | None:intergenic | 30.0% | |
| CACCTGAATTTACTATAGTA+AGG | - | Chr4:48713944-48713963 | None:intergenic | 30.0% | |
| GTTTATCTTACTTCTCTCTA+TGG | + | Chr4:48713369-48713388 | MsG0480020810.01.T01:CDS | 30.0% | |
| TATTGGAGAAAAGGAAAACA+AGG | + | Chr4:48714763-48714782 | MsG0480020810.01.T01:CDS | 30.0% | |
| TGACTCTCTTACAGTAAAAT+GGG | + | Chr4:48714589-48714608 | MsG0480020810.01.T01:CDS | 30.0% | |
| TTGACTCTCTTACAGTAAAA+TGG | + | Chr4:48714588-48714607 | MsG0480020810.01.T01:CDS | 30.0% | |
| TTTGCTTGAAATGGACATAA+GGG | + | Chr4:48714733-48714752 | MsG0480020810.01.T01:CDS | 30.0% | |
| ! | GATTCTATTGAAGGAGTTTT+AGG | + | Chr4:48714860-48714879 | MsG0480020810.01.T01:CDS | 30.0% |
| ! | TTGTTTTCCTTTTCTCCAAT+AGG | - | Chr4:48714764-48714783 | None:intergenic | 30.0% |
| !!! | GAGTTTTAGGTCAAACTTAT+AGG | + | Chr4:48714873-48714892 | MsG0480020810.01.T01:CDS | 30.0% |
| AAATGATAAGGAAGAAGGCA+AGG | + | Chr4:48713456-48713475 | MsG0480020810.01.T01:CDS | 35.0% | |
| AAGGGAAATGATAAGGAAGA+AGG | + | Chr4:48713451-48713470 | MsG0480020810.01.T01:CDS | 35.0% | |
| AATATTGAGCAATGACTCCT+TGG | - | Chr4:48715027-48715046 | None:intergenic | 35.0% | |
| ACACTCCCACAACTTAAAAA+AGG | + | Chr4:48714101-48714120 | MsG0480020810.01.T01:intron | 35.0% | |
| ACTCCTTCAATAGAATCTGT+AGG | - | Chr4:48714857-48714876 | None:intergenic | 35.0% | |
| ATTTCTCCGAGAATGATTGA+TGG | + | Chr4:48714228-48714247 | MsG0480020810.01.T01:intron | 35.0% | |
| CATGTTGTTGAAAAGAGAGA+TGG | - | Chr4:48714971-48714990 | None:intergenic | 35.0% | |
| GAAAGCTCCAAAAAAGAAGA+AGG | + | Chr4:48713504-48713523 | MsG0480020810.01.T01:CDS | 35.0% | |
| GAAAGGAAAAGGAAAAGAGA+AGG | + | Chr4:48713480-48713499 | MsG0480020810.01.T01:CDS | 35.0% | |
| GGTAACAATGGAAATGGTAA+GGG | + | Chr4:48713433-48713452 | MsG0480020810.01.T01:CDS | 35.0% | |
| GTTTGCTTGAAATGGACATA+AGG | + | Chr4:48714732-48714751 | MsG0480020810.01.T01:CDS | 35.0% | |
| TAGCCAATAGAGTAACTCAA+TGG | + | Chr4:48714555-48714574 | MsG0480020810.01.T01:CDS | 35.0% | |
| TCTCTTACAGTAAAATGGGA+CGG | + | Chr4:48714593-48714612 | MsG0480020810.01.T01:CDS | 35.0% | |
| TGATGAAAGAGTAGTAGTTG+TGG | + | Chr4:48714667-48714686 | MsG0480020810.01.T01:CDS | 35.0% | |
| TTCTCGGAGAAATACTTCTA+TGG | - | Chr4:48714221-48714240 | None:intergenic | 35.0% | |
| TTGAAAAGAGAGATGGAGTT+TGG | - | Chr4:48714964-48714983 | None:intergenic | 35.0% | |
| TTGACAGGACTAACATAACT+TGG | - | Chr4:48714899-48714918 | None:intergenic | 35.0% | |
| ! | AGATGATGCTTTTGCTCATT+TGG | + | Chr4:48714808-48714827 | MsG0480020810.01.T01:CDS | 35.0% |
| !! | AGTTTCTGGTTTGCTTGAAA+TGG | + | Chr4:48714724-48714743 | MsG0480020810.01.T01:CDS | 35.0% |
| !! | CAAGGAAATGGTAATGGTAA+CGG | + | Chr4:48713412-48713431 | MsG0480020810.01.T01:CDS | 35.0% |
| !!! | TTTGGAGCCTTCTTCTTTTT+TGG | - | Chr4:48713514-48713533 | None:intergenic | 35.0% |
| AAAGAAGAAGGCTCCAAAAG+AGG | + | Chr4:48713516-48713535 | MsG0480020810.01.T01:CDS | 40.0% | |
| AGAGCAAACTGTGATGGTTA+CGG | + | Chr4:48714323-48714342 | MsG0480020810.01.T01:CDS | 40.0% | |
| AGGAATTGCTATGCCAATGA+TGG | + | Chr4:48714922-48714941 | MsG0480020810.01.T01:CDS | 40.0% | |
| AGTGTTAGAGTCACAGTTTC+TGG | + | Chr4:48714710-48714729 | MsG0480020810.01.T01:CDS | 40.0% | |
| ATAGCAATTCCTCTCTTGAC+AGG | - | Chr4:48714914-48714933 | None:intergenic | 40.0% | |
| CAAATCAACGCACACTTCAT+TGG | + | Chr4:48714449-48714468 | MsG0480020810.01.T01:CDS | 40.0% | |
| CGGTAACAATGGAAATGGTA+AGG | + | Chr4:48713432-48713451 | MsG0480020810.01.T01:CDS | 40.0% | |
| CGTTACCATTACCATTTCCT+TGG | - | Chr4:48713414-48713433 | None:intergenic | 40.0% | |
| CTCAACCGTCAGATTAAGAT+CGG | - | Chr4:48713892-48713911 | None:intergenic | 40.0% | |
| CTTAATCTGACGGTTGAGAT+CGG | + | Chr4:48713894-48713913 | MsG0480020810.01.T01:intron | 40.0% | |
| GACATAAGGGTAAGACCTAT+TGG | + | Chr4:48714746-48714765 | MsG0480020810.01.T01:CDS | 40.0% | |
| GATGAACTTACACTTGCAAG+TGG | - | Chr4:48713722-48713741 | None:intergenic | 40.0% | |
| GGAATTGCTATGCCAATGAT+GGG | + | Chr4:48714923-48714942 | MsG0480020810.01.T01:CDS | 40.0% | |
| GGTAAGACCTATTGGAGAAA+AGG | + | Chr4:48714754-48714773 | MsG0480020810.01.T01:CDS | 40.0% | |
| GTTAGAAGAGCAAACTGTGA+TGG | + | Chr4:48714317-48714336 | MsG0480020810.01.T01:intron | 40.0% | |
| TATGTTAGTCCTGTCAAGAG+AGG | + | Chr4:48714902-48714921 | MsG0480020810.01.T01:CDS | 40.0% | |
| TTAATCTGACGGTTGAGATC+GGG | + | Chr4:48713895-48713914 | MsG0480020810.01.T01:intron | 40.0% | |
| ! | GAATTCGTCGTCTGAAACTA+TGG | - | Chr4:48714429-48714448 | None:intergenic | 40.0% |
| ! | GGTAACGGTAACAATGGAAA+TGG | + | Chr4:48713427-48713446 | MsG0480020810.01.T01:CDS | 40.0% |
| !! | CAATGAAGTGTGCGTTGATT+TGG | - | Chr4:48714451-48714470 | None:intergenic | 40.0% |
| !! | GGTAATGGTAACGGTAACAA+TGG | + | Chr4:48713421-48713440 | MsG0480020810.01.T01:CDS | 40.0% |
| !! | TGAGCAAAAGCATCATCTGA+TGG | - | Chr4:48714806-48714825 | None:intergenic | 40.0% |
| AAGGAAGAAGGCAAGGAGAA+AGG | + | Chr4:48713463-48713482 | MsG0480020810.01.T01:CDS | 45.0% | |
| ACGCACACTTCATTGGTACT+CGG | + | Chr4:48714456-48714475 | MsG0480020810.01.T01:CDS | 45.0% | |
| ATTGCTCAAGGCCAAGGAAA+TGG | + | Chr4:48713400-48713419 | MsG0480020810.01.T01:CDS | 45.0% | |
| CAACAACATGCAAACTCTGC+AGG | + | Chr4:48714981-48715000 | MsG0480020810.01.T01:CDS | 45.0% | |
| CAAGGCCAAGGAAATGGTAA+TGG | + | Chr4:48713406-48713425 | MsG0480020810.01.T01:CDS | 45.0% | |
| CAGTTGCAAACTTTGCCATC+AGG | + | Chr4:48713556-48713575 | MsG0480020810.01.T01:CDS | 45.0% | |
| CTTCCATTCAGCGTCATCAT+CGG | - | Chr4:48714639-48714658 | None:intergenic | 45.0% | |
| GAAGGCAAGGAGAAAGGAAA+AGG | + | Chr4:48713469-48713488 | MsG0480020810.01.T01:CDS | 45.0% | |
| GACGCTGAATGGAAGACTAA+TGG | + | Chr4:48714644-48714663 | MsG0480020810.01.T01:CDS | 45.0% | |
| GGTGTGATGTTCTACTTCCA+TGG | + | Chr4:48714383-48714402 | MsG0480020810.01.T01:CDS | 45.0% | |
| GTCGTCCGATCTTAATCTGA+CGG | + | Chr4:48713884-48713903 | MsG0480020810.01.T01:intron | 45.0% | |
| TATGGCGAAGTTTCCTCCTT+TGG | - | Chr4:48714411-48714430 | None:intergenic | 45.0% | |
| TTCTACTTCCATGGAGCCAA+AGG | + | Chr4:48714392-48714411 | MsG0480020810.01.T01:CDS | 45.0% | |
| TTGTCTTCTCCTCCCATCAT+TGG | - | Chr4:48714938-48714957 | None:intergenic | 45.0% | |
| ! | ATTGCTATGCCAATGATGGG+AGG | + | Chr4:48714926-48714945 | MsG0480020810.01.T01:CDS | 45.0% |
| ! | CTTGTTCTTCTTTGGCTTCC+TGG | - | Chr4:48713656-48713675 | None:intergenic | 45.0% |
| !! | ATTTCCTTGGCCTTGAGCAA+TGG | - | Chr4:48713401-48713420 | None:intergenic | 45.0% |
| AAGGACTCGCGACTACACAT+GGG | + | Chr4:48714487-48714506 | MsG0480020810.01.T01:CDS | 50.0% | |
| ACTGCGTGATTGCATGAACG+CGG | - | Chr4:48713790-48713809 | None:intergenic | 50.0% | |
| AGCAATGACTCCTTGGCTAG+TGG | - | Chr4:48715020-48715039 | None:intergenic | 50.0% | |
| AGCGTCATCATCGGTAGGAA+TGG | - | Chr4:48714630-48714649 | None:intergenic | 50.0% | |
| ATGACTCCTTGGCTAGTGGA+AGG | - | Chr4:48715016-48715035 | None:intergenic | 50.0% | |
| ATGGAAGCTGCCATTGCTCA+AGG | + | Chr4:48713388-48713407 | MsG0480020810.01.T01:CDS | 50.0% | |
| CATTCAGCGTCATCATCGGT+AGG | - | Chr4:48714635-48714654 | None:intergenic | 50.0% | |
| GATCGGGTGACTGCATCAAA+AGG | + | Chr4:48713911-48713930 | MsG0480020810.01.T01:intron | 50.0% | |
| GCAAACTTTGCCATCAGGAC+AGG | + | Chr4:48713561-48713580 | MsG0480020810.01.T01:CDS | 50.0% | |
| TACTTCCATGGAGCCAAAGG+AGG | + | Chr4:48714395-48714414 | MsG0480020810.01.T01:CDS | 50.0% | |
| TGCTACGATCCTCGCTTTGT+TGG | + | Chr4:48714353-48714372 | MsG0480020810.01.T01:CDS | 50.0% | |
| TTCATTGGTACTCGGCCTCA+GGG | + | Chr4:48714464-48714483 | MsG0480020810.01.T01:CDS | 50.0% | |
| ! | AGGAAAGTGGAGCATCTTGG+TGG | + | Chr4:48713342-48713361 | MsG0480020810.01.T01:CDS | 50.0% |
| ! | AGGAGGAAAGTGGAGCATCT+TGG | + | Chr4:48713339-48713358 | MsG0480020810.01.T01:CDS | 50.0% |
| ! | AGTTTCCTCCTTTGGCTCCA+TGG | - | Chr4:48714403-48714422 | None:intergenic | 50.0% |
| ! | CTACCGATGATGACGCTGAA+TGG | + | Chr4:48714633-48714652 | MsG0480020810.01.T01:CDS | 50.0% |
| ! | TACGATCCTCGCTTTGTTGG+TGG | + | Chr4:48714356-48714375 | MsG0480020810.01.T01:CDS | 50.0% |
| !!! | TTCCTGGTTTTGCACTCAGC+TGG | - | Chr4:48713640-48713659 | None:intergenic | 50.0% |
| AATGCTCGCTCCTGTCCTGA+TGG | - | Chr4:48713574-48713593 | None:intergenic | 55.0% | |
| CTTCATTGGTACTCGGCCTC+AGG | + | Chr4:48714463-48714482 | MsG0480020810.01.T01:CDS | 55.0% | |
| GAAGGACTCGCGACTACACA+TGG | + | Chr4:48714486-48714505 | MsG0480020810.01.T01:CDS | 55.0% | |
| GCAAACTCTGCAGGTTCCAG+AGG | + | Chr4:48714990-48715009 | MsG0480020810.01.T01:CDS | 55.0% | |
| GTCCAGCTGAGTGCAAAACC+AGG | + | Chr4:48713635-48713654 | MsG0480020810.01.T01:CDS | 55.0% | |
| ! | CCTCGCTTTGTTGGTGGAGA+CGG | + | Chr4:48714362-48714381 | MsG0480020810.01.T01:CDS | 55.0% |
| CAGAGGCCTTCCACTAGCCA+AGG | + | Chr4:48715007-48715026 | MsG0480020810.01.T01:CDS | 60.0% | |
| CCGTCTCCACCAACAAAGCG+AGG | - | Chr4:48714365-48714384 | None:intergenic | 60.0% | |
| CTTGGCTAGTGGAAGGCCTC+TGG | - | Chr4:48715009-48715028 | None:intergenic | 60.0% | |
| GCTGCCATTGCTCAAGGCCA+AGG | + | Chr4:48713394-48713413 | MsG0480020810.01.T01:CDS | 60.0% | |
| TAGTCGCGAGTCCTTCCCTG+AGG | - | Chr4:48714482-48714501 | None:intergenic | 60.0% | |
| TTGGTACTCGGCCTCAGGGA+AGG | + | Chr4:48714468-48714487 | MsG0480020810.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 48713334 | 48715048 | 48713334 | ID=MsG0480020810.01;Name=MsG0480020810.01 |
| Chr4 | mRNA | 48713334 | 48715048 | 48713334 | ID=MsG0480020810.01.T01;Parent=MsG0480020810.01;Name=MsG0480020810.01.T01;_AED=0.49;_eAED=0.49;_QI=0|0|0|1|1|1|2|0|375 |
| Chr4 | exon | 48713334 | 48713730 | 48713334 | ID=MsG0480020810.01.T01:exon:7374;Parent=MsG0480020810.01.T01 |
| Chr4 | exon | 48714318 | 48715048 | 48714318 | ID=MsG0480020810.01.T01:exon:7375;Parent=MsG0480020810.01.T01 |
| Chr4 | CDS | 48713334 | 48713730 | 48713334 | ID=MsG0480020810.01.T01:cds;Parent=MsG0480020810.01.T01 |
| Chr4 | CDS | 48714318 | 48715048 | 48714318 | ID=MsG0480020810.01.T01:cds;Parent=MsG0480020810.01.T01 |
| Gene Sequence |
| Protein sequence |
