AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280007505.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007505.01.T01 MTR_2g026670 91.852 135 11 0 1 135 1 135 5.66e-57 177
MsG0280007505.01.T01 MTR_4g133900 80.556 72 14 0 4 75 8 79 4.48e-33 117
MsG0280007505.01.T01 MTR_7g056413 73.333 75 20 0 1 75 1 75 2.53e-24 93.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007505.01.T01 AT4G16380 74.324 74 19 0 3 76 7 80 1.03e-32 115
MsG0280007505.01.T01 AT4G16380 72.603 73 20 0 4 76 15 87 1.11e-31 113
MsG0280007505.01.T01 AT1G51090 60.526 76 30 0 1 76 1 76 7.62e-27 98.6
MsG0280007505.01.T01 AT1G49420 48.182 110 45 2 3 111 7 105 1.76e-17 75.1
MsG0280007505.01.T01 AT4G16380 73.333 45 12 0 32 76 15 59 3.76e-16 72.4

Find 43 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACAAAAGTAACTTTCTTTC+TGG 0.091426 2:+16384535 None:intergenic
CTCGGCTTCTTTAGCGGGCT+TGG 0.155696 2:+16384576 None:intergenic
CTTCTCGGGCTCAGCGGACT+TGG 0.187553 2:+16384636 None:intergenic
ACAAGAAATTCAAACCTTAT+AGG 0.213673 2:-16385493 MsG0280007505.01.T01:five_prime_UTR
TTGCTTTGTCATCATACTTT+TGG 0.238102 2:+16384812 None:intergenic
GACTCAGCTGGTTTGGGCTT+TGG 0.238535 2:+16384685 None:intergenic
TTCTCGGACTCAGCTGGTTT+GGG 0.239304 2:+16384679 None:intergenic
CTTCTCGGACTCAGCTGGTT+TGG 0.246232 2:+16384678 None:intergenic
TCTTTCTGGGTCAGCTGTCT+TGG 0.269631 2:+16384549 None:intergenic
CAAGAAATTCAAACCTTATA+GGG 0.285885 2:-16385492 MsG0280007505.01.T01:five_prime_UTR
CTTTCTGGGTCAGCTGTCTT+GGG 0.314272 2:+16384550 None:intergenic
AGCAGAGAAACAACACAAAA+TGG 0.336414 2:+16385409 None:intergenic
CGCTGAGCCCGAGAAGAAAA+AGG 0.353767 2:-16384629 MsG0280007505.01.T01:CDS
TCGGCTTCTTTAGCGGGCTT+GGG 0.362622 2:+16384577 None:intergenic
ACAAAAGTAACTTTCTTTCT+GGG 0.367127 2:+16384536 None:intergenic
GTTGTAGTCCTGAGAAGATT+AGG 0.368435 2:-16384765 MsG0280007505.01.T01:CDS
AGCTGAGTCCGAGAAGAAAA+AGG 0.368826 2:-16384671 MsG0280007505.01.T01:CDS
GGCTTCTCGGCTTCTTTAGC+GGG 0.368935 2:+16384571 None:intergenic
GTCAGCTGTCTTGGGCTTCT+CGG 0.388753 2:+16384558 None:intergenic
ATAAACATGTAGGCCCTATA+AGG 0.393156 2:+16385479 None:intergenic
TTCTCGGGCTCAGCGGACTT+GGG 0.416542 2:+16384637 None:intergenic
TTGTAGTCCTGAGAAGATTA+GGG 0.426247 2:-16384764 MsG0280007505.01.T01:CDS
AGAAATGCTACAAGAAGGTT+AGG 0.427970 2:-16385052 MsG0280007505.01.T01:CDS
TCAGCTGGTTTGGGCTTTGG+CGG 0.463353 2:+16384688 None:intergenic
TATCAAGAGTATTGAAATAG+TGG 0.464638 2:-16384713 MsG0280007505.01.T01:CDS
ATCGACAACCATGGCTGAAA+AGG 0.467264 2:-16385332 MsG0280007505.01.T01:intron
TTATTCAGGTGACCATTATG+AGG 0.467676 2:-16385097 MsG0280007505.01.T01:intron
AGCTTGTCCCTAATCTTCTC+AGG 0.499784 2:+16384757 None:intergenic
ATCAACCTTGAGCCTCATAA+TGG 0.505835 2:+16385085 None:intergenic
GGGCTTCTCGGCTTCTTTAG+CGG 0.509630 2:+16384570 None:intergenic
AGGGACAAGCTTTGTTACAA+AGG 0.533858 2:-16384745 MsG0280007505.01.T01:CDS
TGCATAGAGTCAATAAGGTT+AGG 0.536861 2:-16385682 None:intergenic
AAGCTTTGTTACAAAGGTGG+AGG 0.555529 2:-16384739 MsG0280007505.01.T01:CDS
GACAAGCTTTGTTACAAAGG+TGG 0.583958 2:-16384742 MsG0280007505.01.T01:CDS
GGTGACCATTATGAGGCTCA+AGG 0.605189 2:-16385090 MsG0280007505.01.T01:CDS
GTGTGAGAAATGCTACAAGA+AGG 0.610703 2:-16385057 MsG0280007505.01.T01:CDS
AGATTGTTGATAGACAGTGT+TGG 0.619844 2:+16385518 None:intergenic
AGCTTAATACATAAACATGT+AGG 0.630199 2:+16385469 None:intergenic
AGCAAACATTGTGACAATCA+CGG 0.650689 2:-16384794 MsG0280007505.01.T01:CDS
AGATCTCTGATCGACAACCA+TGG 0.651264 2:-16385341 MsG0280007505.01.T01:exon
TAGGCGTGTAAATCAAAAGA+CGG 0.656958 2:-16385663 MsG0280007505.01.T01:five_prime_UTR
TCGATCAGAGATCTGATCTG+AGG 0.666948 2:+16385350 None:intergenic
AAACATTGTGACAATCACGG+TGG 0.804631 2:-16384791 MsG0280007505.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAACAATTAAAATTATACAT+AGG + Chr2:16385078-16385097 None:intergenic 15.0%
!!! ATATAAATTGAGCTAATTTT+TGG - Chr2:16385326-16385345 MsG0280007505.01.T01:intron 15.0%
!!! TATAAATTGAGCTAATTTTT+GGG - Chr2:16385327-16385346 MsG0280007505.01.T01:intron 15.0%
!!! TATTGTTTTATTAGAATTTG+TGG - Chr2:16385249-16385268 MsG0280007505.01.T01:intron 15.0%
!!! TTAATTGTTGATTTTTATTC+AGG - Chr2:16385088-16385107 MsG0280007505.01.T01:CDS 15.0%
!!! TTATATCCTTTTCATTTTTT+TGG + Chr2:16385312-16385331 None:intergenic 15.0%
!! AATAAAAGAATGTCTTCATA+TGG + Chr2:16385027-16385046 None:intergenic 20.0%
! AAATTATACATAGGACCATA+TGG + Chr2:16385069-16385088 None:intergenic 25.0%
! AACAAAAGTAACTTTCTTTC+TGG + Chr2:16385667-16385686 None:intergenic 25.0%
! ACAAAAGTAACTTTCTTTCT+GGG + Chr2:16385666-16385685 None:intergenic 25.0%
! ACAAGAAATTCAAACCTTAT+AGG - Chr2:16384706-16384725 MsG0280007505.01.T01:CDS 25.0%
! AGCTTAATACATAAACATGT+AGG + Chr2:16384733-16384752 None:intergenic 25.0%
! ATAGTTAGAAATGAAAGTAG+AGG + Chr2:16385207-16385226 None:intergenic 25.0%
! CAAGAAATTCAAACCTTATA+GGG - Chr2:16384707-16384726 MsG0280007505.01.T01:CDS 25.0%
! GGATTACCAAAAAAATGAAA+AGG - Chr2:16385303-16385322 MsG0280007505.01.T01:intron 25.0%
! TAAAAACACTTGTATGCATT+TGG - Chr2:16385282-16385301 MsG0280007505.01.T01:intron 25.0%
!! TCATAAATTTGTTCACCATA+TGG - Chr2:16385051-16385070 MsG0280007505.01.T01:CDS 25.0%
!!! TATCAAGAGTATTGAAATAG+TGG - Chr2:16385486-16385505 MsG0280007505.01.T01:five_prime_UTR 25.0%
! TGACAATCATGTGTTTGTTT+TGG + Chr2:16384939-16384958 None:intergenic 30.0%
! TTGCTTTGTCATCATACTTT+TGG + Chr2:16385390-16385409 None:intergenic 30.0%
AGAAATGCTACAAGAAGGTT+AGG - Chr2:16385147-16385166 MsG0280007505.01.T01:intron 35.0%
AGCAAACATTGTGACAATCA+CGG - Chr2:16385405-16385424 MsG0280007505.01.T01:five_prime_UTR 35.0%
AGCAGAGAAACAACACAAAA+TGG + Chr2:16384793-16384812 None:intergenic 35.0%
ATAAACATGTAGGCCCTATA+AGG + Chr2:16384723-16384742 None:intergenic 35.0%
GAACCAGAGAAGAAAAAAGA+AGG - Chr2:16385592-16385611 MsG0280007505.01.T01:five_prime_UTR 35.0%
TAGGCGTGTAAATCAAAAGA+CGG - Chr2:16384536-16384555 MsG0280007505.01.T01:CDS 35.0%
TTATTCAGGTGACCATTATG+AGG - Chr2:16385102-16385121 MsG0280007505.01.T01:intron 35.0%
TTGTAGTCCTGAGAAGATTA+GGG - Chr2:16385435-16385454 MsG0280007505.01.T01:five_prime_UTR 35.0%
! AGATTGTTGATAGACAGTGT+TGG + Chr2:16384684-16384703 None:intergenic 35.0%
! GGTTTTTGCTAGAACTGAAA+GGG + Chr2:16384663-16384682 None:intergenic 35.0%
! TGGTTTTTGCTAGAACTGAA+AGG + Chr2:16384664-16384683 None:intergenic 35.0%
AAACATTGTGACAATCACGG+TGG - Chr2:16385408-16385427 MsG0280007505.01.T01:five_prime_UTR 40.0%
ATCAACCTTGAGCCTCATAA+TGG + Chr2:16385117-16385136 None:intergenic 40.0%
GTGTGAGAAATGCTACAAGA+AGG - Chr2:16385142-16385161 MsG0280007505.01.T01:intron 40.0%
GTTGTAGTCCTGAGAAGATT+AGG - Chr2:16385434-16385453 MsG0280007505.01.T01:five_prime_UTR 40.0%
! ACACTTTACCTTTTCAGCCA+TGG + Chr2:16384878-16384897 None:intergenic 40.0%
!! AAGCTTTGTTACAAAGGTGG+AGG - Chr2:16385460-16385479 MsG0280007505.01.T01:five_prime_UTR 40.0%
!! AGGGACAAGCTTTGTTACAA+AGG - Chr2:16385454-16385473 MsG0280007505.01.T01:five_prime_UTR 40.0%
!! CTCTGGTTCCTTTTTCTTCT+CGG + Chr2:16385581-16385600 None:intergenic 40.0%
!! GACAAGCTTTGTTACAAAGG+TGG - Chr2:16385457-16385476 MsG0280007505.01.T01:five_prime_UTR 40.0%
!! TCTGGTTCCTTTTTCTTCTC+GGG + Chr2:16385580-16385599 None:intergenic 40.0%
!!! TCGCCTTCTTTTTTCTTCTC+TGG + Chr2:16385598-16385617 None:intergenic 40.0%
AGATCTCTGATCGACAACCA+TGG - Chr2:16384858-16384877 MsG0280007505.01.T01:intron 45.0%
AGCTGAGTCCGAGAAGAAAA+AGG - Chr2:16385528-16385547 MsG0280007505.01.T01:five_prime_UTR 45.0%
AGCTTGTCCCTAATCTTCTC+AGG + Chr2:16385445-16385464 None:intergenic 45.0%
ATCGACAACCATGGCTGAAA+AGG - Chr2:16384867-16384886 MsG0280007505.01.T01:intron 45.0%
GAGTCCGAGAAGAAAAAGGA+AGG - Chr2:16385532-16385551 MsG0280007505.01.T01:five_prime_UTR 45.0%
TAGAGGTGATGAACTCACGA+GGG + Chr2:16385190-16385209 None:intergenic 45.0%
TCGATCAGAGATCTGATCTG+AGG + Chr2:16384852-16384871 None:intergenic 45.0%
! CTCGCCTTCCTTTTTCTTCT+CGG + Chr2:16385539-16385558 None:intergenic 45.0%
! TTTTTCTTCTCGGACTCAGC+TGG + Chr2:16385529-16385548 None:intergenic 45.0%
CTTTCTGGGTCAGCTGTCTT+GGG + Chr2:16385652-16385671 None:intergenic 50.0%
GGTGACCATTATGAGGCTCA+AGG - Chr2:16385109-16385128 MsG0280007505.01.T01:intron 50.0%
GTAGAGGTGATGAACTCACG+AGG + Chr2:16385191-16385210 None:intergenic 50.0%
TCTTTCTGGGTCAGCTGTCT+TGG + Chr2:16385653-16385672 None:intergenic 50.0%
! CTTTTTCTTCTCGGGCTCAG+CGG + Chr2:16385572-16385591 None:intergenic 50.0%
! TTCTCGGACTCAGCTGGTTT+GGG + Chr2:16385523-16385542 None:intergenic 50.0%
CGCTGAGCCCGAGAAGAAAA+AGG - Chr2:16385570-16385589 MsG0280007505.01.T01:five_prime_UTR 55.0%
CTTCTCGGACTCAGCTGGTT+TGG + Chr2:16385524-16385543 None:intergenic 55.0%
GGCTTCTCGGCTTCTTTAGC+GGG + Chr2:16385631-16385650 None:intergenic 55.0%
GGGCTTCTCGGCTTCTTTAG+CGG + Chr2:16385632-16385651 None:intergenic 55.0%
GTCAGCTGTCTTGGGCTTCT+CGG + Chr2:16385644-16385663 None:intergenic 55.0%
!! GACTCAGCTGGTTTGGGCTT+TGG + Chr2:16385517-16385536 None:intergenic 55.0%
!! TCAGCTGGTTTGGGCTTTGG+CGG + Chr2:16385514-16385533 None:intergenic 55.0%
!! TCGGCTTCTTTAGCGGGCTT+GGG + Chr2:16385625-16385644 None:intergenic 55.0%
! TTCTCGGGCTCAGCGGACTT+GGG + Chr2:16385565-16385584 None:intergenic 60.0%
!! CTCGGCTTCTTTAGCGGGCT+TGG + Chr2:16385626-16385645 None:intergenic 60.0%
! CTTCTCGGGCTCAGCGGACT+TGG + Chr2:16385566-16385585 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr2 gene 16384528 16385693 16384528 ID=MsG0280007505.01;Name=MsG0280007505.01
Chr2 mRNA 16384528 16385693 16384528 ID=MsG0280007505.01.T01;Parent=MsG0280007505.01;Name=MsG0280007505.01.T01;_AED=0.31;_eAED=0.31;_QI=349|1|1|1|1|1|3|0|136
Chr2 exon 16384528 16384844 16384528 ID=MsG0280007505.01.T01:exon:1237;Parent=MsG0280007505.01.T01
Chr2 exon 16385030 16385111 16385030 ID=MsG0280007505.01.T01:exon:1236;Parent=MsG0280007505.01.T01
Chr2 exon 16385333 16385693 16385333 ID=MsG0280007505.01.T01:exon:1235;Parent=MsG0280007505.01.T01
Chr2 five_prime_UTR 16385345 16385693 16385345 ID=MsG0280007505.01.T01:five_prime_utr;Parent=MsG0280007505.01.T01
Chr2 CDS 16385333 16385344 16385333 ID=MsG0280007505.01.T01:cds;Parent=MsG0280007505.01.T01
Chr2 CDS 16385030 16385111 16385030 ID=MsG0280007505.01.T01:cds;Parent=MsG0280007505.01.T01
Chr2 CDS 16384528 16384844 16384528 ID=MsG0280007505.01.T01:cds;Parent=MsG0280007505.01.T01
Gene Sequence

>MsG0280007505.01.T01

ATGGCTGAAAAGGTGACCATTATGAGGCTCAAGGTTGATCTTGAGTGTGAGAAATGCTACAAGAAGGTTAGGAAGCTTCTCAACAAGTACCCTCAAATTCGAGACCAAAAGTATGATGACAAAGCAAACATTGTGACAATCACGGTGGTATGTTGTAGTCCTGAGAAGATTAGGGACAAGCTTTGTTACAAAGGTGGAGGTTCTATCAAGAGTATTGAAATAGTGGATCCGCCAAAGCCCAAACCAGCTGAGTCCGAGAAGAAAAAGGAAGGCGAGAAGCCCAAGTCCGCTGAGCCCGAGAAGAAAAAGGAACCAGAGAAGAAAAAAGAAGGCGAGAAGCCCAAGCCCGCTAAAGAAGCCGAGAAGCCCAAGACAGCTGACCCAGAAAGAAAGTTACTTTTGTTTCTGTAG

Protein sequence

>MsG0280007505.01.T01

MAEKVTIMRLKVDLECEKCYKKVRKLLNKYPQIRDQKYDDKANIVTITVVCCSPEKIRDKLCYKGGGSIKSIEIVDPPKPKPAESEKKKEGEKPKSAEPEKKKEPEKKKEGEKPKPAKEAEKPKTADPERKLLLFL*