AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880045796.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045796.01.T01 MTR_8g079120 95.769 260 11 0 1 260 1 260 0.0 525
MsG0880045796.01.T01 MTR_8g079120 94.643 168 9 0 1 168 43 210 3.44e-118 337
MsG0880045796.01.T01 MTR_5g018365 62.302 252 95 0 9 260 10 261 2.78e-116 334
MsG0880045796.01.T01 MTR_5g018350 59.200 250 102 0 11 260 7 256 2.74e-108 313
MsG0880045796.01.T01 MTR_5g018300 42.912 261 144 2 5 260 2 262 2.03e-73 225
MsG0880045796.01.T01 MTR_5g018320 45.525 257 131 4 11 260 5 259 9.45e-73 223
MsG0880045796.01.T01 MTR_8g007675 43.191 257 137 5 11 260 32 286 3.14e-68 213
MsG0880045796.01.T01 MTR_3g464040 41.634 257 140 4 10 260 18 270 3.86e-66 207
MsG0880045796.01.T01 MTR_5g018280 40.856 257 146 3 9 260 6 261 3.96e-65 204
MsG0880045796.01.T01 MTR_8g008660 42.023 257 139 5 10 260 26 278 7.38e-63 199
MsG0880045796.01.T01 MTR_8g008505 41.313 259 140 5 10 260 27 281 4.82e-61 194
MsG0880045796.01.T01 MTR_8g007670 40.385 260 145 5 9 260 5 262 3.87e-60 191
MsG0880045796.01.T01 MTR_8g008500 38.996 259 150 5 10 260 26 284 9.87e-59 188
MsG0880045796.01.T01 MTR_8g007670 39.062 256 110 5 9 260 5 218 4.26e-55 177
MsG0880045796.01.T01 MTR_7g015220 33.730 252 158 3 11 257 124 371 3.40e-37 135
MsG0880045796.01.T01 MTR_3g005410 33.077 260 158 5 7 257 90 342 2.10e-36 132
MsG0880045796.01.T01 MTR_3g093730 32.927 246 153 5 7 246 32 271 4.99e-34 124
MsG0880045796.01.T01 MTR_5g068100 31.395 258 166 4 9 260 28 280 5.00e-34 124
MsG0880045796.01.T01 MTR_3g093730 31.148 244 115 5 7 246 32 226 4.83e-30 113
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045796.01.T01 AT2G23620 54.086 257 114 2 9 261 7 263 3.05e-102 298
MsG0880045796.01.T01 AT2G23610 52.734 256 117 3 9 260 7 262 4.58e-94 278
MsG0880045796.01.T01 AT2G23600 52.964 253 115 2 9 257 7 259 5.33e-94 277
MsG0880045796.01.T01 AT2G23600 52.964 253 115 2 9 257 22 274 5.78e-94 278
MsG0880045796.01.T01 AT2G23590 47.638 254 128 1 7 260 23 271 1.23e-88 264
MsG0880045796.01.T01 AT4G37150 48.627 255 126 2 10 260 2 255 6.40e-88 262
MsG0880045796.01.T01 AT2G23580 49.805 257 124 2 9 260 6 262 1.03e-87 261
MsG0880045796.01.T01 AT5G10300 49.027 257 125 2 9 261 4 258 9.06e-87 259
MsG0880045796.01.T01 AT2G23550 48.638 257 127 2 9 260 6 262 1.38e-83 251
MsG0880045796.01.T01 AT2G23550 48.638 257 127 2 9 260 13 269 1.67e-83 251
MsG0880045796.01.T01 AT2G23560 46.063 254 135 2 9 260 6 259 6.61e-80 241
MsG0880045796.01.T01 AT2G23610 46.640 253 92 2 9 260 7 217 1.13e-77 234
MsG0880045796.01.T01 AT3G50440 43.478 253 139 2 12 260 22 274 1.08e-72 224
MsG0880045796.01.T01 AT2G23550 45.312 256 108 2 9 260 6 233 8.80e-70 215
MsG0880045796.01.T01 AT2G23600 52.439 164 74 2 9 168 22 185 7.38e-59 185
MsG0880045796.01.T01 AT2G23570 48.315 178 88 1 87 260 1 178 6.79e-53 170
MsG0880045796.01.T01 AT2G23580 48.045 179 85 3 9 182 6 181 9.62e-53 170
MsG0880045796.01.T01 AT2G23550 47.273 165 82 2 9 168 13 177 3.27e-50 164
MsG0880045796.01.T01 AT5G10300 47.590 166 81 2 9 170 4 167 2.05e-47 157
MsG0880045796.01.T01 AT4G37140 53.846 117 54 0 10 126 2 118 3.09e-45 149
MsG0880045796.01.T01 AT4G09900 33.074 257 156 5 10 257 97 346 1.84e-39 140
MsG0880045796.01.T01 AT1G26360 32.669 251 164 2 10 257 188 436 4.37e-39 141
MsG0880045796.01.T01 AT1G33990 32.443 262 164 5 3 257 90 345 5.01e-39 139
MsG0880045796.01.T01 AT3G29770 35.294 255 150 5 11 257 138 385 1.64e-37 136
MsG0880045796.01.T01 AT5G58310 32.700 263 160 5 7 257 1 258 2.57e-37 132
MsG0880045796.01.T01 AT4G37140 58.242 91 38 0 10 100 2 92 4.43e-37 127
MsG0880045796.01.T01 AT1G69240 31.518 257 161 6 10 257 186 436 1.73e-36 134
MsG0880045796.01.T01 AT4G16690 31.707 246 153 4 11 249 12 249 3.32e-33 122
MsG0880045796.01.T01 AT3G10870 30.738 244 157 5 11 246 20 259 9.26e-31 115
MsG0880045796.01.T01 AT3G29770 40.278 72 43 0 11 82 138 209 2.85e-12 65.5

Find 67 sgRNAs with CRISPR-Local

Find 211 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCATTAATCCCAGCATTT+TGG 0.157812 8:+64591501 None:intergenic
GAGCTTATTGGACAAGAATT+TGG 0.163249 8:+64591857 None:intergenic
GAGGCCAGGGTCCCTCTTTA+TGG 0.202954 8:-64591639 MsG0880045796.01.T01:CDS
ATTCTTGACCTTTCAGCTTC+TGG 0.238179 8:-64594442 MsG0880045796.01.T01:CDS
AACAAAACATCAATGTTCTT+TGG 0.296879 8:-64591882 MsG0880045796.01.T01:CDS
GCAAGATAACTTCTCTAAAC+AGG 0.300900 8:-64591603 MsG0880045796.01.T01:CDS
AGCTTATTGGACAAGAATTT+GGG 0.339561 8:+64591858 None:intergenic
GGAGTGTTGGTAGAGCTTAT+TGG 0.344380 8:+64591845 None:intergenic
AACTTCTCTAAACAGGGTTA+TGG 0.360676 8:-64591596 MsG0880045796.01.T01:CDS
ACTTCTCTAAACAGGGTTAT+GGG 0.399672 8:-64591595 MsG0880045796.01.T01:CDS
AATGGAACAATTTCCAGAAA+AGG 0.417631 8:-64594278 MsG0880045796.01.T01:CDS
ATTCTTGTGGGTCATAGTCT+TGG 0.418009 8:-64594322 MsG0880045796.01.T01:CDS
ATCCAGTGTTGAAATTTCAA+TGG 0.421026 8:+64591527 None:intergenic
GATTTAGCAGGATCTTGAAT+TGG 0.423617 8:-64591675 MsG0880045796.01.T01:intron
TGAACCATTGTTGAAGCTAA+TGG 0.426644 8:-64594371 MsG0880045796.01.T01:CDS
ATCTTCAAGTTTCTTCAAGT+TGG 0.431463 8:+64594417 None:intergenic
GTGCACAAAATTTAATTGCT+AGG 0.449601 8:-64594558 MsG0880045796.01.T01:CDS
TGCTTCTTATGTCATGGAAA+AGG 0.451368 8:-64594206 MsG0880045796.01.T01:intron
CACTGGATGATCCAAAATGC+TGG 0.456893 8:-64591512 MsG0880045796.01.T01:CDS
GATCAAAGGGGCAGATCATA+TGG 0.460208 8:-64591471 MsG0880045796.01.T01:CDS
TGTCCAACCATTCAGCCGTC+GGG 0.460887 8:+64591925 None:intergenic
ATGATGTATATGAGATCAAA+GGG 0.461047 8:-64591484 MsG0880045796.01.T01:CDS
CTTGTGGGTCATAGTCTTGG+AGG 0.464347 8:-64594319 MsG0880045796.01.T01:CDS
ATGGTGCTTGCCATGGTGCT+TGG 0.470545 8:-64594516 MsG0880045796.01.T01:CDS
TTCCATTGAAATTTCAACAC+TGG 0.470781 8:-64591529 MsG0880045796.01.T01:CDS
GCCAAGACTTTATTGAGGCC+AGG 0.472046 8:-64591653 MsG0880045796.01.T01:CDS
CACAATTCCACCAAATGAAA+AGG 0.475812 8:-64594347 MsG0880045796.01.T01:CDS
CTTGCCATGGTGCTTGGAGT+TGG 0.476673 8:-64594510 MsG0880045796.01.T01:CDS
GAAGCATTGTGTTGAATGTC+CGG 0.482394 8:+64594223 None:intergenic
CCCTGGCCTCAATAAAGTCT+TGG 0.486588 8:+64591652 None:intergenic
ACACAATGCTTCTTATGTCA+TGG 0.491092 8:-64594212 MsG0880045796.01.T01:CDS
AATGAAAAGGTGATTCTTGT+GGG 0.497441 8:-64594334 MsG0880045796.01.T01:CDS
CTTGTACATGGTGCTTGCCA+TGG 0.498303 8:-64594523 MsG0880045796.01.T01:CDS
TACCCCGACGGCTGAATGGT+TGG 0.505808 8:-64591928 MsG0880045796.01.T01:CDS
AGAGTACCCCGACGGCTGAA+TGG 0.512702 8:-64591932 MsG0880045796.01.T01:CDS
ATTGTTTGCAATGAAGACCT+TGG 0.520269 8:-64591554 MsG0880045796.01.T01:CDS
AGAGAATTACATAGTTCTTG+TGG 0.522605 8:+64591434 None:intergenic
CCAAGACTTTATTGAGGCCA+GGG 0.522924 8:-64591652 MsG0880045796.01.T01:CDS
GAAATTTCAATGGAATTCCA+AGG 0.525367 8:+64591537 None:intergenic
GTGTCCAACCATTCAGCCGT+CGG 0.529692 8:+64591924 None:intergenic
AAGCATTATGTTCTTGTACA+TGG 0.531450 8:-64594535 MsG0880045796.01.T01:CDS
TTTCCAGAAAAGGTTGCTGT+TGG 0.543459 8:-64594268 MsG0880045796.01.T01:CDS
AATGATGTATATGAGATCAA+AGG 0.543714 8:-64591485 MsG0880045796.01.T01:CDS
AAATGAAAAGGTGATTCTTG+TGG 0.544198 8:-64594335 MsG0880045796.01.T01:CDS
AATTGGCCAAGACTTTATTG+AGG 0.557607 8:-64591658 MsG0880045796.01.T01:CDS
TGGTGCTTGGAGTTGGTACA+AGG 0.561033 8:-64594503 MsG0880045796.01.T01:CDS
GTGGCCATTAGCTTCAACAA+TGG 0.573860 8:+64594367 None:intergenic
GAGTCAAGTCTTCCATAAAG+AGG 0.579389 8:+64591627 None:intergenic
AGTTGGTTCCAGAAGCTGAA+AGG 0.584796 8:+64594434 None:intergenic
AAGCCACGTTTAGAATCTGC+TGG 0.586149 8:-64594478 MsG0880045796.01.T01:CDS
TTACCTCAGTGGAGGAGTGT+TGG 0.588942 8:+64591832 None:intergenic
ACTGGATGATCCAAAATGCT+GGG 0.589459 8:-64591511 MsG0880045796.01.T01:CDS
CAAGATAACTTCTCTAAACA+GGG 0.605122 8:-64591602 MsG0880045796.01.T01:CDS
ACTTAGCATAGCTCTTGCAA+TGG 0.605259 8:-64594296 MsG0880045796.01.T01:CDS
AGTCAAGTCTTCCATAAAGA+GGG 0.612508 8:+64591628 None:intergenic
TTTAGAATCTGCTGGTCATG+TGG 0.623515 8:-64594470 MsG0880045796.01.T01:CDS
GACACTGAATTCTGCCAGTG+TGG 0.627184 8:-64591906 MsG0880045796.01.T01:CDS
TCTTCCATAAAGAGGGACCC+TGG 0.632528 8:+64591635 None:intergenic
GTCCAACCATTCAGCCGTCG+GGG 0.648788 8:+64591926 None:intergenic
ACGTGTAGTTACCTCAGTGG+AGG 0.650895 8:+64591824 None:intergenic
TTAACGTGTAGTTACCTCAG+TGG 0.666283 8:+64591821 None:intergenic
TTGCAAACAATAAAAGCACG+TGG 0.692906 8:+64591566 None:intergenic
TGTACCAACTCCAAGCACCA+TGG 0.696634 8:+64594506 None:intergenic
TGACCAGCAGATTCTAAACG+TGG 0.719220 8:+64594475 None:intergenic
GTATATTGAGAGTACCCCGA+CGG 0.725457 8:-64591940 MsG0880045796.01.T01:CDS
TGATGTATATGAGATCAAAG+GGG 0.769291 8:-64591483 MsG0880045796.01.T01:CDS
CTACCAACACTCCTCCACTG+AGG 0.776664 8:-64591835 MsG0880045796.01.T01:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAAAAAATAAGATTAATAA+TGG + Chr8:64593994-64594013 None:intergenic 10.0%
!! ACATATAATAAAACATAAAA+AGG + Chr8:64592924-64592943 None:intergenic 10.0%
!! ATATAATAAAACATAAAAAG+GGG + Chr8:64592922-64592941 None:intergenic 10.0%
!! CATATAATAAAACATAAAAA+GGG + Chr8:64592923-64592942 None:intergenic 10.0%
!! TTATAACAAAATTTAGATTA+GGG - Chr8:64593439-64593458 MsG0880045796.01.T01:intron 10.0%
!! TTTATAACAAAATTTAGATT+AGG - Chr8:64593438-64593457 MsG0880045796.01.T01:intron 10.0%
!!! AAATACATTTTATTCATTTA+TGG + Chr8:64593285-64593304 None:intergenic 10.0%
!!! CATAATAAGTAATTTTTATT+TGG + Chr8:64593369-64593388 None:intergenic 10.0%
!! ATACATTTAGTATACAAAAT+TGG + Chr8:64592685-64592704 None:intergenic 15.0%
!! CCTATATTTATAAAAACAAA+TGG + Chr8:64593625-64593644 None:intergenic 15.0%
!! GAAAATAAATTAAAAAGTTG+TGG + Chr8:64592104-64592123 None:intergenic 15.0%
!! TACTTCAGAAAATAATATAA+GGG + Chr8:64592297-64592316 None:intergenic 15.0%
!! TATAATAAAACATAAAAAGG+GGG + Chr8:64592921-64592940 None:intergenic 15.0%
!! TTACTTCAGAAAATAATATA+AGG + Chr8:64592298-64592317 None:intergenic 15.0%
!!! ATAAGTCTTATTTAATCTAA+CGG + Chr8:64591970-64591989 None:intergenic 15.0%
!!! CCATTTGTTTTTATAAATAT+AGG - Chr8:64593622-64593641 MsG0880045796.01.T01:intron 15.0%
!!! GAATTTGTAAAATTTTGTTA+TGG - Chr8:64592410-64592429 MsG0880045796.01.T01:intron 15.0%
!!! TAGTTTTTATGAAATTTTGT+AGG - Chr8:64591888-64591907 MsG0880045796.01.T01:CDS 15.0%
!! ATAATAAAACATAAAAAGGG+GGG + Chr8:64592920-64592939 None:intergenic 20.0%
!! ATTTGTCCTTTACAATAAAA+TGG - Chr8:64592962-64592981 MsG0880045796.01.T01:intron 20.0%
!! TCACAAATATGATTTCTATA+TGG - Chr8:64591844-64591863 MsG0880045796.01.T01:CDS 20.0%
!! TCATACAAGAAAAATAGATT+AGG - Chr8:64593662-64593681 MsG0880045796.01.T01:intron 20.0%
!! TGACAATTAAAAAGTAATGA+AGG + Chr8:64593844-64593863 None:intergenic 20.0%
!! TTGAAGTTTCCATTAAAAAA+TGG - Chr8:64594232-64594251 MsG0880045796.01.T01:CDS 20.0%
!!! AATTGTACTCCATTTTTTAA+TGG + Chr8:64594244-64594263 None:intergenic 20.0%
!!! AGTTAAATTCTTGATACTTT+TGG + Chr8:64593114-64593133 None:intergenic 20.0%
!!! TAACTATTTAGCATTTTTGT+CGG - Chr8:64592823-64592842 MsG0880045796.01.T01:intron 20.0%
!!! TGGAAAATTTGAGTTATAAA+AGG + Chr8:64592324-64592343 None:intergenic 20.0%
!!! TTTTATGAAATTTTGTAGGT+GGG - Chr8:64591892-64591911 MsG0880045796.01.T01:CDS 20.0%
!!! TTTTCCATTTTTTTTGTCAA+TGG + Chr8:64592249-64592268 None:intergenic 20.0%
!!! TTTTTATGAAATTTTGTAGG+TGG - Chr8:64591891-64591910 MsG0880045796.01.T01:CDS 20.0%
! AACAAAACATCAATGTTCTT+TGG - Chr8:64594086-64594105 MsG0880045796.01.T01:intron 25.0%
! AATGATGTATATGAGATCAA+AGG - Chr8:64594483-64594502 MsG0880045796.01.T01:CDS 25.0%
! ACTAATAAAGACAAACTACT+TGG - Chr8:64594271-64594290 MsG0880045796.01.T01:CDS 25.0%
! AGAAGAACATGTAATAGAAA+AGG + Chr8:64592878-64592897 None:intergenic 25.0%
! ATATAAAACGACAAGTAAGT+AGG + Chr8:64593780-64593799 None:intergenic 25.0%
! ATCACCATTGACAAAAAAAA+TGG - Chr8:64592242-64592261 MsG0880045796.01.T01:intron 25.0%
! ATGATGTATATGAGATCAAA+GGG - Chr8:64594484-64594503 MsG0880045796.01.T01:CDS 25.0%
! CATTAATTACTGAAGAGATA+TGG - Chr8:64593945-64593964 MsG0880045796.01.T01:intron 25.0%
! CATTTAGTATACAAAATTGG+TGG + Chr8:64592682-64592701 None:intergenic 25.0%
! GAAGAACATGTAATAGAAAA+GGG + Chr8:64592877-64592896 None:intergenic 25.0%
! TCAATGGTGATATTTATTGT+GGG + Chr8:64592233-64592252 None:intergenic 25.0%
!! TCTAAGACAATTAAGTAAAC+TGG + Chr8:64593883-64593902 None:intergenic 25.0%
!! TTCCTCTTAATTTGAATTTG+TGG - Chr8:64593407-64593426 MsG0880045796.01.T01:intron 25.0%
!!! AAAATTTTGTTATGGAACGA+AGG - Chr8:64592418-64592437 MsG0880045796.01.T01:intron 25.0%
!!! AATTTTAAAACGACAAGCAA+GGG + Chr8:64593917-64593936 None:intergenic 25.0%
AAAGCAAAACTTTCCAGTTT+CGG + Chr8:64592990-64593009 None:intergenic 30.0%
AAATGAAAAGGTGATTCTTG+TGG - Chr8:64591633-64591652 MsG0880045796.01.T01:CDS 30.0%
AAGCATTATGTTCTTGTACA+TGG - Chr8:64591433-64591452 MsG0880045796.01.T01:CDS 30.0%
AATGAAAAGGTGATTCTTGT+GGG - Chr8:64591634-64591653 MsG0880045796.01.T01:CDS 30.0%
AATGGAACAATTTCCAGAAA+AGG - Chr8:64591690-64591709 MsG0880045796.01.T01:intron 30.0%
AATTGTCACATTTACACTTG+TGG - Chr8:64593856-64593875 MsG0880045796.01.T01:intron 30.0%
AGAGAATTACATAGTTCTTG+TGG + Chr8:64594537-64594556 None:intergenic 30.0%
AGCTTATTGGACAAGAATTT+GGG + Chr8:64594113-64594132 None:intergenic 30.0%
AGGACACTTAAAAAGATATC+GGG - Chr8:64592772-64592791 MsG0880045796.01.T01:intron 30.0%
CAAGATAACTTCTCTAAACA+GGG - Chr8:64594366-64594385 MsG0880045796.01.T01:CDS 30.0%
CAGGACACTTAAAAAGATAT+CGG - Chr8:64592771-64592790 MsG0880045796.01.T01:intron 30.0%
GAAAGAACATTAATTCAGTG+TGG + Chr8:64592013-64592032 None:intergenic 30.0%
GAAATTTCAATGGAATTCCA+AGG + Chr8:64594434-64594453 None:intergenic 30.0%
GACCACAAATTCAAATTAAG+AGG + Chr8:64593412-64593431 None:intergenic 30.0%
GTAAAAAAACCATGTCTCTA+GGG + Chr8:64592529-64592548 None:intergenic 30.0%
GTCAATGGTGATATTTATTG+TGG + Chr8:64592234-64592253 None:intergenic 30.0%
GTGCACAAAATTTAATTGCT+AGG - Chr8:64591410-64591429 MsG0880045796.01.T01:CDS 30.0%
TATAGTACTAGTATGAGACA+AGG + Chr8:64594186-64594205 None:intergenic 30.0%
TCGAGTACTTACATTAGAAA+AGG + Chr8:64592133-64592152 None:intergenic 30.0%
TGACATGAGAAAGACATTAA+TGG + Chr8:64592201-64592220 None:intergenic 30.0%
TGACTTGAATTCAAGTAAGT+AGG + Chr8:64593736-64593755 None:intergenic 30.0%
TGATGTATATGAGATCAAAG+GGG - Chr8:64594485-64594504 MsG0880045796.01.T01:CDS 30.0%
TGATTCTCTCAACTTTACAT+GGG + Chr8:64593475-64593494 None:intergenic 30.0%
TGCAGTTGACACAAATTAAA+AGG + Chr8:64593593-64593612 None:intergenic 30.0%
TTCCATTGAAATTTCAACAC+TGG - Chr8:64594439-64594458 MsG0880045796.01.T01:CDS 30.0%
TTCTAATGTAAGTACTCGAT+AGG - Chr8:64592134-64592153 MsG0880045796.01.T01:intron 30.0%
! AAATATTTACAGAGTGTCTC+AGG - Chr8:64592752-64592771 MsG0880045796.01.T01:intron 30.0%
! ACAAGAATCACCTTTTCATT+TGG + Chr8:64591634-64591653 None:intergenic 30.0%
! ATCCAGTGTTGAAATTTCAA+TGG + Chr8:64594444-64594463 None:intergenic 30.0%
! ATCTTCAAGTTTCTTCAAGT+TGG + Chr8:64591554-64591573 None:intergenic 30.0%
! ATTCGTTCGAGTTTAAATTC+AGG - Chr8:64593195-64593214 MsG0880045796.01.T01:intron 30.0%
! TATTTAGCATTTTTGTCGGT+GGG - Chr8:64592827-64592846 MsG0880045796.01.T01:intron 30.0%
! TCAATTTTTAACCCAGACAA+TGG - Chr8:64592486-64592505 MsG0880045796.01.T01:intron 30.0%
! TCGGTACCATTTTATTGTAA+AGG + Chr8:64592971-64592990 None:intergenic 30.0%
! TTTCTTAACAGCTTTTATGC+CGG - Chr8:64591726-64591745 MsG0880045796.01.T01:intron 30.0%
!! GGGGGAAAAGTATATTTTAA+GGG + Chr8:64592902-64592921 None:intergenic 30.0%
!! TTGATACTTTTGGAAACGAT+AGG + Chr8:64593104-64593123 None:intergenic 30.0%
!!! GAATTTTAAAACGACAAGCA+AGG + Chr8:64593918-64593937 None:intergenic 30.0%
AACTTCTCTAAACAGGGTTA+TGG - Chr8:64594372-64594391 MsG0880045796.01.T01:CDS 35.0%
AAGACTTATGCGATCTCATT+AGG - Chr8:64591981-64592000 MsG0880045796.01.T01:intron 35.0%
AATTGGCCAAGACTTTATTG+AGG - Chr8:64594310-64594329 MsG0880045796.01.T01:CDS 35.0%
ACAAACTACTTGGATTTAGC+AGG - Chr8:64594281-64594300 MsG0880045796.01.T01:CDS 35.0%
ACACAATGCTTCTTATGTCA+TGG - Chr8:64591756-64591775 MsG0880045796.01.T01:intron 35.0%
ACTGAAGAGATATGGAATTG+AGG - Chr8:64593953-64593972 MsG0880045796.01.T01:intron 35.0%
ACTTCTCTAAACAGGGTTAT+GGG - Chr8:64594373-64594392 MsG0880045796.01.T01:CDS 35.0%
AGTAGGACTATTGAGGTTTA+TGG + Chr8:64593719-64593738 None:intergenic 35.0%
AGTCAAGTCTTCCATAAAGA+GGG + Chr8:64594343-64594362 None:intergenic 35.0%
ATACAAAATTGGTGGAGTCT+AGG + Chr8:64592674-64592693 None:intergenic 35.0%
ATTGCAATCTTCATTGACGT+TGG + Chr8:64593498-64593517 None:intergenic 35.0%
ATTGTTTGCAATGAAGACCT+TGG - Chr8:64594414-64594433 MsG0880045796.01.T01:CDS 35.0%
ATTTCACCATCGAAGCATAA+TGG - Chr8:64592377-64592396 MsG0880045796.01.T01:intron 35.0%
CACAATTCCACCAAATGAAA+AGG - Chr8:64591621-64591640 MsG0880045796.01.T01:CDS 35.0%
CATGAGAAAGACATTAATGG+AGG + Chr8:64592198-64592217 None:intergenic 35.0%
CTGAAGAGATATGGAATTGA+GGG - Chr8:64593954-64593973 MsG0880045796.01.T01:intron 35.0%
GAGATATCATACTCTCTACT+TGG - Chr8:64593253-64593272 MsG0880045796.01.T01:intron 35.0%
GAGCTTATTGGACAAGAATT+TGG + Chr8:64594114-64594133 None:intergenic 35.0%
GATTTAGCAGGATCTTGAAT+TGG - Chr8:64594293-64594312 MsG0880045796.01.T01:CDS 35.0%
GCAAGATAACTTCTCTAAAC+AGG - Chr8:64594365-64594384 MsG0880045796.01.T01:CDS 35.0%
GGACACTTAAAAAGATATCG+GGG - Chr8:64592773-64592792 MsG0880045796.01.T01:intron 35.0%
GGTAAAAAAACCATGTCTCT+AGG + Chr8:64592530-64592549 None:intergenic 35.0%
GTGATTCTCTCAACTTTACA+TGG + Chr8:64593476-64593495 None:intergenic 35.0%
TACAAAATTGGTGGAGTCTA+GGG + Chr8:64592673-64592692 None:intergenic 35.0%
TATTTCCACAACCATTGTCT+GGG + Chr8:64592500-64592519 None:intergenic 35.0%
TCAAGTAAGTAGGACTATTG+AGG + Chr8:64593726-64593745 None:intergenic 35.0%
TGAAAGAAACTGATGCCTAA+AGG + Chr8:64593062-64593081 None:intergenic 35.0%
TGAACCATTGTTGAAGCTAA+TGG - Chr8:64591597-64591616 MsG0880045796.01.T01:CDS 35.0%
TGAATTTCCATTTGTGTGGA+TGG - Chr8:64592443-64592462 MsG0880045796.01.T01:intron 35.0%
TGCTTCTTATGTCATGGAAA+AGG - Chr8:64591762-64591781 MsG0880045796.01.T01:intron 35.0%
TGTTGGATAGCGATAATTCA+AGG + Chr8:64593016-64593035 None:intergenic 35.0%
TTATTTCCACAACCATTGTC+TGG + Chr8:64592501-64592520 None:intergenic 35.0%
TTGGAAACGATAGGTAAGTA+GGG + Chr8:64593095-64593114 None:intergenic 35.0%
TTTCACCATCGAAGCATAAT+GGG - Chr8:64592378-64592397 MsG0880045796.01.T01:intron 35.0%
TTTGGAAACGATAGGTAAGT+AGG + Chr8:64593096-64593115 None:intergenic 35.0%
! AGAATCACCTTTTCATTTGG+TGG + Chr8:64591631-64591650 None:intergenic 35.0%
! CAATAAAATGGTACCGAAAC+TGG - Chr8:64592974-64592993 MsG0880045796.01.T01:intron 35.0%
! CATAGATGTGATTTGTGTTC+TGG + Chr8:64593559-64593578 None:intergenic 35.0%
! CATCATTAATCCCAGCATTT+TGG + Chr8:64594470-64594489 None:intergenic 35.0%
! CTATTTAGCATTTTTGTCGG+TGG - Chr8:64592826-64592845 MsG0880045796.01.T01:intron 35.0%
! GAAGCATAATGGGAAAAATC+TGG - Chr8:64592388-64592407 MsG0880045796.01.T01:intron 35.0%
! TCAGTGTTTCTGAGTATTCA+CGG + Chr8:64592062-64592081 None:intergenic 35.0%
! TTGCAAACAATAAAAGCACG+TGG + Chr8:64594405-64594424 None:intergenic 35.0%
! TTTTTCCCATTATGCTTCGA+TGG + Chr8:64592386-64592405 None:intergenic 35.0%
!! AAATTTTGTAGGTGGGTTCT+AGG - Chr8:64591899-64591918 MsG0880045796.01.T01:CDS 35.0%
!! AATCTAACGGTTAAGTTGAG+TGG + Chr8:64591957-64591976 None:intergenic 35.0%
!! AATTTTGTAGGTGGGTTCTA+GGG - Chr8:64591900-64591919 MsG0880045796.01.T01:CDS 35.0%
!! CTTTTCATTTGGTGGAATTG+TGG + Chr8:64591623-64591642 None:intergenic 35.0%
!! GGGGGGAAAAGTATATTTTA+AGG + Chr8:64592903-64592922 None:intergenic 35.0%
!!! ACATGGTTTTTTTACCAGTG+AGG - Chr8:64592534-64592553 MsG0880045796.01.T01:intron 35.0%
!!! CATGGTTTTTTTACCAGTGA+GGG - Chr8:64592535-64592554 MsG0880045796.01.T01:intron 35.0%
!!! TTGATGTTTTGTTTCCACAC+TGG + Chr8:64594079-64594098 None:intergenic 35.0%
ACTGGATGATCCAAAATGCT+GGG - Chr8:64594457-64594476 MsG0880045796.01.T01:CDS 40.0%
ACTTAGCATAGCTCTTGCAA+TGG - Chr8:64591672-64591691 MsG0880045796.01.T01:intron 40.0%
ATTCTTGACCTTTCAGCTTC+TGG - Chr8:64591526-64591545 MsG0880045796.01.T01:CDS 40.0%
ATTCTTGTGGGTCATAGTCT+TGG - Chr8:64591646-64591665 MsG0880045796.01.T01:CDS 40.0%
CGTGCTTGATATTGAAGATG+CGG - Chr8:64592347-64592366 MsG0880045796.01.T01:intron 40.0%
CTTCAATATCAAGCACGCAA+TGG + Chr8:64592344-64592363 None:intergenic 40.0%
GAGTCAAGTCTTCCATAAAG+AGG + Chr8:64594344-64594363 None:intergenic 40.0%
GGACTGAATTTCCATTTGTG+TGG - Chr8:64592439-64592458 MsG0880045796.01.T01:intron 40.0%
GTGGAGTAACACACATTTGT+TGG + Chr8:64593033-64593052 None:intergenic 40.0%
TTAACGTGTAGTTACCTCAG+TGG + Chr8:64594150-64594169 None:intergenic 40.0%
TTACTCCACAGTTCACCTTT+AGG - Chr8:64593044-64593063 MsG0880045796.01.T01:intron 40.0%
TTTAACCCAGACAATGGTTG+TGG - Chr8:64592492-64592511 MsG0880045796.01.T01:intron 40.0%
TTTCCAGAAAAGGTTGCTGT+TGG - Chr8:64591700-64591719 MsG0880045796.01.T01:intron 40.0%
! TTTAGAATCTGCTGGTCATG+TGG - Chr8:64591498-64591517 MsG0880045796.01.T01:CDS 40.0%
!! GAAGCATTGTGTTGAATGTC+CGG + Chr8:64591748-64591767 None:intergenic 40.0%
!!! ATGGTTTTTTCGAACCTTGC+AGG + Chr8:64593700-64593719 None:intergenic 40.0%
ATAACCTGACCCTAGAGACA+TGG - Chr8:64592517-64592536 MsG0880045796.01.T01:intron 45.0%
CACTGGATGATCCAAAATGC+TGG - Chr8:64594456-64594475 MsG0880045796.01.T01:CDS 45.0%
CCAAGACTTTATTGAGGCCA+GGG - Chr8:64594316-64594335 MsG0880045796.01.T01:CDS 45.0%
GATCAAAGGGGCAGATCATA+TGG - Chr8:64594497-64594516 MsG0880045796.01.T01:CDS 45.0%
TGACCAGCAGATTCTAAACG+TGG + Chr8:64591496-64591515 None:intergenic 45.0%
TGATGCCTAAAGGTGAACTG+TGG + Chr8:64593052-64593071 None:intergenic 45.0%
TGGTTGTGATCCAGTGTAGA+CGG - Chr8:64592565-64592584 MsG0880045796.01.T01:intron 45.0%
TTACCAGTGAGGGAAGAAGA+TGG - Chr8:64592545-64592564 MsG0880045796.01.T01:intron 45.0%
TTGTTGTACACCGTCTACAC+TGG + Chr8:64592578-64592597 None:intergenic 45.0%
! AAGCCACGTTTAGAATCTGC+TGG - Chr8:64591490-64591509 MsG0880045796.01.T01:CDS 45.0%
! ACACCAACAGCAACCTTTTC+TGG + Chr8:64591706-64591725 None:intergenic 45.0%
! AGTTGGTTCCAGAAGCTGAA+AGG + Chr8:64591537-64591556 None:intergenic 45.0%
! GTGGCCATTAGCTTCAACAA+TGG + Chr8:64591604-64591623 None:intergenic 45.0%
! GTGTTTCTGAGTATTCACGG+TGG + Chr8:64592059-64592078 None:intergenic 45.0%
!! AAAACCATGTCTCTAGGGTC+AGG + Chr8:64592524-64592543 None:intergenic 45.0%
!! AGCATTTTTGTCGGTGGGTT+AGG - Chr8:64592832-64592851 MsG0880045796.01.T01:intron 45.0%
!! GGAGTGTTGGTAGAGCTTAT+TGG + Chr8:64594126-64594145 None:intergenic 45.0%
!! GTATATTGAGAGTACCCCGA+CGG - Chr8:64594028-64594047 MsG0880045796.01.T01:intron 45.0%
!!! AAATTATTCTTTAAAAAAAT+CGG + Chr8:64592723-64592742 None:intergenic 5.0%
!!! TAAATTTTGTTATAAAAAAA+TGG + Chr8:64593434-64593453 None:intergenic 5.0%
ACGTGTAGTTACCTCAGTGG+AGG + Chr8:64594147-64594166 None:intergenic 50.0%
CAACCATCTTCTTCCCTCAC+TGG + Chr8:64592551-64592570 None:intergenic 50.0%
CACTCGTCCATCCACACAAA+TGG + Chr8:64592453-64592472 None:intergenic 50.0%
CCCTGGCCTCAATAAAGTCT+TGG + Chr8:64594319-64594338 None:intergenic 50.0%
CTTGTACATGGTGCTTGCCA+TGG - Chr8:64591445-64591464 MsG0880045796.01.T01:CDS 50.0%
GACACTGAATTCTGCCAGTG+TGG - Chr8:64594062-64594081 MsG0880045796.01.T01:intron 50.0%
GCCAAGACTTTATTGAGGCC+AGG - Chr8:64594315-64594334 MsG0880045796.01.T01:CDS 50.0%
GCCTCTTAATAGTGCTCCAC+CGG - Chr8:64592624-64592643 MsG0880045796.01.T01:intron 50.0%
GGAGAAACACATCTCCTGCA+AGG - Chr8:64593683-64593702 MsG0880045796.01.T01:intron 50.0%
GGCTCTATTAAGTCCCTCAC+CGG - Chr8:64591927-64591946 MsG0880045796.01.T01:CDS 50.0%
GGTCCAATTAAGTCCCTCAC+CGG + Chr8:64592646-64592665 None:intergenic 50.0%
TAATAGTGCTCCACCGGTGA+GGG - Chr8:64592630-64592649 MsG0880045796.01.T01:intron 50.0%
TCTATTAAGTCCCTCACCGG+TGG - Chr8:64591930-64591949 MsG0880045796.01.T01:CDS 50.0%
TCTTCCATAAAGAGGGACCC+TGG + Chr8:64594336-64594355 None:intergenic 50.0%
TGTACCAACTCCAAGCACCA+TGG + Chr8:64591465-64591484 None:intergenic 50.0%
TTAATAGTGCTCCACCGGTG+AGG - Chr8:64592629-64592648 MsG0880045796.01.T01:intron 50.0%
! AATTGGTGGAGTCTAGGGTG+AGG + Chr8:64592668-64592687 None:intergenic 50.0%
! ACCGGTGGAGCACTATTAAG+AGG + Chr8:64592628-64592647 None:intergenic 50.0%
! ATTGGTGGAGTCTAGGGTGA+GGG + Chr8:64592667-64592686 None:intergenic 50.0%
! CTTGTGGGTCATAGTCTTGG+AGG - Chr8:64591649-64591668 MsG0880045796.01.T01:CDS 50.0%
! TTACCTCAGTGGAGGAGTGT+TGG + Chr8:64594139-64594158 None:intergenic 50.0%
!! GGTTAAGTTGAGTGGTCCAC+CGG + Chr8:64591949-64591968 None:intergenic 50.0%
!! TGGTGCTTGGAGTTGGTACA+AGG - Chr8:64591465-64591484 MsG0880045796.01.T01:CDS 50.0%
ATGGTGCTTGCCATGGTGCT+TGG - Chr8:64591452-64591471 MsG0880045796.01.T01:CDS 55.0%
CACGGTGGAGAGAGTGAGAT+TGG + Chr8:64592044-64592063 None:intergenic 55.0%
CCAATTAAGTCCCTCACCGG+TGG + Chr8:64592643-64592662 None:intergenic 55.0%
CCACCGGTGAGGGACTTAAT+TGG - Chr8:64592640-64592659 MsG0880045796.01.T01:intron 55.0%
CTACCAACACTCCTCCACTG+AGG - Chr8:64594133-64594152 MsG0880045796.01.T01:intron 55.0%
CTTGCCATGGTGCTTGGAGT+TGG - Chr8:64591458-64591477 MsG0880045796.01.T01:CDS 55.0%
GTGTCCAACCATTCAGCCGT+CGG + Chr8:64594047-64594066 None:intergenic 55.0%
TGTCCAACCATTCAGCCGTC+GGG + Chr8:64594046-64594065 None:intergenic 55.0%
!! GTAGGTGGGTTCTAGGGTGA+GGG - Chr8:64591906-64591925 MsG0880045796.01.T01:CDS 55.0%
!! TGTAGGTGGGTTCTAGGGTG+AGG - Chr8:64591905-64591924 MsG0880045796.01.T01:CDS 55.0%
AGTTGAGTGGTCCACCGGTG+AGG + Chr8:64591944-64591963 None:intergenic 60.0%
GAGGCCAGGGTCCCTCTTTA+TGG - Chr8:64594329-64594348 MsG0880045796.01.T01:CDS 60.0%
GTCCAACCATTCAGCCGTCG+GGG + Chr8:64594045-64594064 None:intergenic 60.0%
GTTGAGTGGTCCACCGGTGA+GGG + Chr8:64591943-64591962 None:intergenic 60.0%
TACCCCGACGGCTGAATGGT+TGG - Chr8:64594040-64594059 MsG0880045796.01.T01:intron 60.0%
! AGAGTACCCCGACGGCTGAA+TGG - Chr8:64594036-64594055 MsG0880045796.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr8 gene 64591406 64594584 64591406 ID=MsG0880045796.01;Name=MsG0880045796.01
Chr8 mRNA 64591406 64594584 64591406 ID=MsG0880045796.01.T01;Parent=MsG0880045796.01;Name=MsG0880045796.01.T01;_AED=0.47;_eAED=0.48;_QI=0|0|0|1|1|1|3|0|261
Chr8 exon 64594207 64594584 64594207 ID=MsG0880045796.01.T01:exon:24492;Parent=MsG0880045796.01.T01
Chr8 exon 64591836 64591961 64591836 ID=MsG0880045796.01.T01:exon:24491;Parent=MsG0880045796.01.T01
Chr8 exon 64591406 64591687 64591406 ID=MsG0880045796.01.T01:exon:24490;Parent=MsG0880045796.01.T01
Chr8 CDS 64594207 64594584 64594207 ID=MsG0880045796.01.T01:cds;Parent=MsG0880045796.01.T01
Chr8 CDS 64591836 64591961 64591836 ID=MsG0880045796.01.T01:cds;Parent=MsG0880045796.01.T01
Chr8 CDS 64591406 64591687 64591406 ID=MsG0880045796.01.T01:cds;Parent=MsG0880045796.01.T01
Gene Sequence

>MsG0880045796.01.T01

ATGAGTGCACAAAATTTAATTGCTAGGAAGCATTATGTTCTTGTACATGGTGCTTGCCATGGTGCTTGGAGTTGGTACAAGGTCAAGCCACGTTTAGAATCTGCTGGTCATGTGGTCACAATTCTTGACCTTTCAGCTTCTGGAACCAACTTGAAGAAACTTGAAGATGTTGATACAATTTCAGAATACTCTGAACCATTGTTGAAGCTAATGGCCACAATTCCACCAAATGAAAAGGTGATTCTTGTGGGTCATAGTCTTGGAGGACTTAGCATAGCTCTTGCAATGGAACAATTTCCAGAAAAGGTTGCTGTTGGTGTTTTCTTAACAGCTTTTATGCCGGACATTCAACACAATGCTTCTTATGTCATGGAAAAGTATATTGAGAGTACCCCGACGGCTGAATGGTTGGACACTGAATTCTGCCAGTGTGGAAACAAAACATCAATGTTCTTTGGTCCCAAATTCTTGTCCAATAAGCTCTACCAACACTCCTCCACTGAGGATCTTGAATTGGCCAAGACTTTATTGAGGCCAGGGTCCCTCTTTATGGAAGACTTGACTCAGCAAGATAACTTCTCTAAACAGGGTTATGGGTCAGTTCCACGTGCTTTTATTGTTTGCAATGAAGACCTTGGAATTCCATTGAAATTTCAACACTGGATGATCCAAAATGCTGGGATTAATGATGTATATGAGATCAAAGGGGCAGATCATATGGCTATGATTTGCAAGCCACAAGAACTATGTAATTCTCTCAATCAGATAGCTTTAAAATACTTGTGA
Protein sequence

>MsG0880045796.01.T01

MSAQNLIARKHYVLVHGACHGAWSWYKVKPRLESAGHVVTILDLSASGTNLKKLEDVDTISEYSEPLLKLMATIPPNEKVILVGHSLGGLSIALAMEQFPEKVAVGVFLTAFMPDIQHNASYVMEKYIESTPTAEWLDTEFCQCGNKTSMFFGPKFLSNKLYQHSSTEDLELAKTLLRPGSLFMEDLTQQDNFSKQGYGSVPRAFIVCNEDLGIPLKFQHWMIQNAGINDVYEIKGADHMAMICKPQELCNSLNQIALKYL*