AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880046598.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046598.01.T01 MTR_4g024850 88.473 347 6 1 1 313 1 347 0.0 617
MsG0880046598.01.T01 MTR_4g111780 87.896 347 8 1 1 313 1 347 0.0 611
MsG0880046598.01.T01 MTR_4g124560 30.868 311 178 8 15 302 42 338 1.70e-29 115
MsG0880046598.01.T01 MTR_2g093050 27.974 311 177 7 36 300 27 336 2.49e-24 102
MsG0880046598.01.T01 MTR_1g102670 30.717 293 170 7 8 280 19 298 2.42e-23 98.2
MsG0880046598.01.T01 MTR_2g445230 27.907 258 138 8 90 300 20 276 3.87e-17 80.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046598.01.T01 AT2G45280 64.841 347 83 3 1 313 22 363 1.68e-150 427
MsG0880046598.01.T01 AT2G45280 60.377 371 84 4 1 313 22 387 5.22e-144 411
MsG0880046598.01.T01 AT3G22880 30.915 317 185 7 8 303 39 342 2.80e-30 117
MsG0880046598.01.T01 AT3G22880 31.148 305 176 7 8 291 39 330 1.20e-27 110
MsG0880046598.01.T01 AT5G20850 29.450 309 181 8 15 300 43 337 2.54e-27 109
MsG0880046598.01.T01 AT5G57450 34.066 182 91 4 89 241 21 202 5.56e-25 102
MsG0880046598.01.T01 AT5G57450 34.066 182 91 4 89 241 21 202 5.56e-25 102
MsG0880046598.01.T01 AT2G28560 27.879 330 183 10 21 300 12 336 2.01e-24 102
MsG0880046598.01.T01 AT2G28560 27.879 330 183 10 21 300 12 336 2.57e-24 101
MsG0880046598.01.T01 AT2G28560 27.879 330 183 10 21 300 12 336 2.57e-24 101
MsG0880046598.01.T01 AT2G28560 27.879 330 183 10 21 300 12 336 2.57e-24 101
MsG0880046598.01.T01 AT2G28560 29.304 273 146 7 74 300 33 304 2.93e-24 101
MsG0880046598.01.T01 AT5G20850 29.643 280 161 7 15 272 43 308 4.24e-24 100
MsG0880046598.01.T01 AT2G28560 30.102 196 111 5 74 244 33 227 1.41e-16 78.2

Find 78 sgRNAs with CRISPR-Local

Find 223 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAATCGATCAGTTATAAA+TGG 0.178807 8:-75323183 None:intergenic
AGATACTGAAGGAAGCTTTA+TGG 0.268343 8:+75321246 MsG0880046598.01.T01:intron
CACCATACTCTGTGACTTCT+AGG 0.288453 8:+75322994 MsG0880046598.01.T01:CDS
CCGCCAAGACTTTGATGATA+TGG 0.290126 8:+75321729 MsG0880046598.01.T01:CDS
ACCTAGGCCACCATAGTCTA+AGG 0.294287 8:-75321119 None:intergenic
GGCATCGGTAAAACACAAAT+AGG 0.294764 8:+75320961 MsG0880046598.01.T01:CDS
GTTCAGATTCCCTTAGACTA+TGG 0.313027 8:+75321109 MsG0880046598.01.T01:CDS
TTTGTCCAAATCACTACTTC+TGG 0.324317 8:-75319863 None:intergenic
TTATGGCCTTCAAAGGATAC+TGG 0.333562 8:+75323106 MsG0880046598.01.T01:three_prime_UTR
AATTGCGTTGGTAAATTACT+TGG 0.346991 8:+75321423 MsG0880046598.01.T01:CDS
TGCAACCAGAAGTAGTGATT+TGG 0.352806 8:+75319858 MsG0880046598.01.T01:CDS
CCCCTAGAAGTCACAGAGTA+TGG 0.353043 8:-75322996 None:intergenic
TGCAAAGAAGTGACTGAAAT+TGG 0.359942 8:+75320805 MsG0880046598.01.T01:CDS
AATTTCTGAGAGTGAAGCTT+TGG 0.371631 8:+75319822 MsG0880046598.01.T01:CDS
CCTCTCTTTGATGCCGAGAT+TGG 0.378039 8:-75319462 None:intergenic
CGAACCGGATCATCTTATAT+TGG 0.390394 8:+75322913 MsG0880046598.01.T01:CDS
CCCTTAGACTATGGTGGCCT+AGG 0.427121 8:+75321118 MsG0880046598.01.T01:CDS
ACTAACACTAATGTTATTCA+TGG 0.443602 8:+75319895 MsG0880046598.01.T01:CDS
GTGTAGGTGGTCAGACTGCT+TGG 0.447156 8:+75320698 MsG0880046598.01.T01:intron
TTTCAATTGACCCTTGCCTT+AGG 0.450193 8:+75322588 MsG0880046598.01.T01:CDS
GTTACAACTAAGCATATTGA+AGG 0.450439 8:+75322561 MsG0880046598.01.T01:CDS
GGTCGCATTCGTGCACGAAC+CGG 0.464764 8:+75322898 MsG0880046598.01.T01:CDS
CATCAAAGTCTTGGCGGAAG+TGG 0.467042 8:-75321723 None:intergenic
TTTGAAGGCCATAAAAGAAA+AGG 0.471474 8:-75323097 None:intergenic
AGAGTGGTCGACATGGAAAT+CGG 0.480430 8:+75319432 MsG0880046598.01.T01:exon
GCATCGGTAAAACACAAATA+GGG 0.480592 8:+75320962 MsG0880046598.01.T01:CDS
CTTGATCTAGATAACATCCT+TGG 0.482948 8:+75320769 MsG0880046598.01.T01:CDS
TCAATCGATCAGTTATAAAT+GGG 0.483919 8:-75323182 None:intergenic
GCTCTCCGGACTCGATTACT+TGG 0.488443 8:+75321751 MsG0880046598.01.T01:CDS
CAAAAGCCAGTATCCTTTGA+AGG 0.489333 8:-75323112 None:intergenic
ACCATACTCTGTGACTTCTA+GGG 0.489593 8:+75322995 MsG0880046598.01.T01:CDS
TATTTATTTAGGTGGAGTCC+CGG 0.493765 8:+75320939 MsG0880046598.01.T01:intron
AATCGGAATGCTACCAATCT+CGG 0.507178 8:+75319449 MsG0880046598.01.T01:CDS
CATTCCAATATAAGATGATC+CGG 0.514566 8:-75322917 None:intergenic
AGTGGTGGAAGTGCGAAGAA+TGG 0.524949 8:-75319520 None:intergenic
ATCTAGATCAAGACAAGAAG+TGG 0.525367 8:-75320758 None:intergenic
CTAGATAACATCCTTGGTGG+AGG 0.531137 8:+75320775 MsG0880046598.01.T01:CDS
CGGATCATCTTATATTGGAA+TGG 0.531446 8:+75322918 MsG0880046598.01.T01:CDS
GAGCCATATCATCAAAGTCT+TGG 0.534652 8:-75321732 None:intergenic
AATAAAAGTTACCTAAGGCA+AGG 0.538816 8:-75322599 None:intergenic
TTAGGTGGAGTCCCGGGCAT+CGG 0.539291 8:+75320946 MsG0880046598.01.T01:intron
TGTATATTGCCTTCCCACCT+AGG 0.543573 8:-75321135 None:intergenic
AGAGGAAAGCTCTTAGCAGC+TGG 0.543939 8:+75319480 MsG0880046598.01.T01:CDS
TCCACCACTCACCTTGCTCG+AGG 0.547898 8:+75319534 MsG0880046598.01.T01:CDS
CAGATTCCCTTAGACTATGG+TGG 0.548711 8:+75321112 MsG0880046598.01.T01:CDS
TTTCACCAAGTAATCGAGTC+CGG 0.557484 8:-75321756 None:intergenic
CCATATCATCAAAGTCTTGG+CGG 0.560623 8:-75321729 None:intergenic
ATTTATTTAGGTGGAGTCCC+GGG 0.561610 8:+75320940 MsG0880046598.01.T01:intron
TAGACTATGGTGGCCTAGGT+GGG 0.562545 8:+75321122 MsG0880046598.01.T01:CDS
ACCTCGAGCAAGGTGAGTGG+TGG 0.569638 8:-75319535 None:intergenic
AAGCAGTAAAAGAATCAAGA+TGG 0.571650 8:+75323037 MsG0880046598.01.T01:CDS
TGTAGGTGGTCAGACTGCTT+GGG 0.572676 8:+75320699 MsG0880046598.01.T01:intron
ATAAAAGTTACCTAAGGCAA+GGG 0.578617 8:-75322598 None:intergenic
CTATGGTGGCCTAGGTGGGA+AGG 0.579059 8:+75321126 MsG0880046598.01.T01:CDS
AATGCTGAACAGGTGATAGC+TGG 0.579437 8:+75322877 MsG0880046598.01.T01:intron
CTATACTGAACAAATTGCGT+TGG 0.579516 8:+75321411 MsG0880046598.01.T01:CDS
TTAGACTATGGTGGCCTAGG+TGG 0.582042 8:+75321121 MsG0880046598.01.T01:CDS
ACTTTGATGATATGGCTCTC+CGG 0.582235 8:+75321737 MsG0880046598.01.T01:CDS
GCTGAAGCTGATTTAAGAGA+AGG 0.582838 8:-75322972 None:intergenic
TGCAATTAATCCCTCCACCA+AGG 0.588385 8:-75320786 None:intergenic
GATCTAGATAACATCCTTGG+TGG 0.590736 8:+75320772 MsG0880046598.01.T01:CDS
TGAAAATGAAACCTCGAGCA+AGG 0.593132 8:-75319545 None:intergenic
CCAATCTCGGCATCAAAGAG+AGG 0.613185 8:+75319462 MsG0880046598.01.T01:CDS
ACGAAAGAGAGTGGTCGACA+TGG 0.618889 8:+75319425 MsG0880046598.01.T01:exon
CCATACTCTGTGACTTCTAG+GGG 0.623155 8:+75322996 MsG0880046598.01.T01:CDS
AGTGATTTGGACAAATCAAG+TGG 0.626621 8:+75319871 MsG0880046598.01.T01:CDS
TTACTTGGACAAATTCGTAA+CGG 0.626860 8:+75321438 MsG0880046598.01.T01:CDS
TAGATAACATCCTTGGTGGA+GGG 0.628191 8:+75320776 MsG0880046598.01.T01:CDS
GCATGATACTCAGATACTGA+AGG 0.630770 8:+75321235 MsG0880046598.01.T01:intron
CCTAGGCCACCATAGTCTAA+GGG 0.645792 8:-75321118 None:intergenic
TGCTTGGGATATGCTCAATG+AGG 0.650417 8:+75320714 MsG0880046598.01.T01:CDS
TGGAAGTGCGAAGAATGGAG+TGG 0.664143 8:-75319515 None:intergenic
GAAACCTCGAGCAAGGTGAG+TGG 0.680004 8:-75319538 None:intergenic
CACAAAAGTTCACTATACTG+AGG 0.683168 8:-75323140 None:intergenic
GTGATTTGGACAAATCAAGT+GGG 0.687675 8:+75319872 MsG0880046598.01.T01:CDS
GGTGGGAAGGCAATATACAT+CGG 0.695624 8:+75321139 MsG0880046598.01.T01:CDS
GGAAAAGTAACGAAAGAGAG+TGG 0.695917 8:+75319416 MsG0880046598.01.T01:five_prime_UTR
CATACTCTGTGACTTCTAGG+GGG 0.769105 8:+75322997 MsG0880046598.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTTAGTTTATTTATTTATTT+AGG + Chr8:75320928-75320947 MsG0880046598.01.T01:intron 10.0%
!!! AAACTTTTTAACATTTAAAA+GGG - Chr8:75319588-75319607 None:intergenic 10.0%
!!! TAAACTTTTTAACATTTAAA+AGG - Chr8:75319589-75319608 None:intergenic 10.0%
!! AGTTTATTTATTTATTTAGG+TGG + Chr8:75320931-75320950 MsG0880046598.01.T01:intron 15.0%
!! CAAAAACATGTAAATTTAAT+TGG - Chr8:75320865-75320884 None:intergenic 15.0%
!!! AACTTTTTAACATTTAAAAG+GGG - Chr8:75319587-75319606 None:intergenic 15.0%
!!! GATATCTTTTTGTTATTATA+TGG + Chr8:75319652-75319671 MsG0880046598.01.T01:intron 15.0%
!!! TATTCAATTGATATTTCTTT+TGG + Chr8:75321490-75321509 MsG0880046598.01.T01:intron 15.0%
!!! TTTTCTCCATTTTTTTTTTT+GGG + Chr8:75320673-75320692 MsG0880046598.01.T01:intron 15.0%
!! AAAAAAAATTGCATACACAA+GGG - Chr8:75320634-75320653 None:intergenic 20.0%
!! AAACTAGAACTCTATATATT+AGG + Chr8:75321985-75322004 MsG0880046598.01.T01:intron 20.0%
!! AAATTAATAGACATTCTGTT+TGG + Chr8:75320282-75320301 MsG0880046598.01.T01:intron 20.0%
!! AATAGAGAAGAAAACTATTA+AGG - Chr8:75320890-75320909 None:intergenic 20.0%
!! ACATTAGAACTATTTATACA+TGG - Chr8:75320021-75320040 None:intergenic 20.0%
!! GTTCATTTGAATAGTAAATT+CGG - Chr8:75322777-75322796 None:intergenic 20.0%
!! TAGAAACATGAAATAGAAAT+CGG - Chr8:75321004-75321023 None:intergenic 20.0%
!! TTCATTTGAATAGTAAATTC+GGG - Chr8:75322776-75322795 None:intergenic 20.0%
!!! AATTAAGTATTTAGAGCATA+AGG - Chr8:75322355-75322374 None:intergenic 20.0%
!!! ACTTTTTAACATTTAAAAGG+GGG - Chr8:75319586-75319605 None:intergenic 20.0%
!!! ATATTTTCCAAGATACTATT+CGG - Chr8:75321370-75321389 None:intergenic 20.0%
!!! GTTTTCTCCATTTTTTTTTT+TGG + Chr8:75320672-75320691 MsG0880046598.01.T01:intron 20.0%
!!! TAGACTTAAAAGTATTCATT+TGG - Chr8:75322211-75322230 None:intergenic 20.0%
!!! TGAATACTTTTAAGTCTATT+TGG + Chr8:75322213-75322232 MsG0880046598.01.T01:intron 20.0%
!!! TGCTAACTTTATACAATTTT+GGG - Chr8:75319624-75319643 None:intergenic 20.0%
!!! TTATGTTAAATTTTGATTGC+AGG + Chr8:75322521-75322540 MsG0880046598.01.T01:intron 20.0%
!!! TTCTTTTAATGCATTGTTAT+AGG + Chr8:75321669-75321688 MsG0880046598.01.T01:intron 20.0%
!!! TTGCAATATTGATAATATGA+AGG + Chr8:75319681-75319700 MsG0880046598.01.T01:intron 20.0%
!!! TTGCTAACTTTATACAATTT+TGG - Chr8:75319625-75319644 None:intergenic 20.0%
! AAACTACATATGTATTCCAA+TGG - Chr8:75320575-75320594 None:intergenic 25.0%
! AATCTTCACATACTTAACAA+AGG - Chr8:75320206-75320225 None:intergenic 25.0%
! ACACAACCCAAAAAAAAAAA+TGG - Chr8:75320682-75320701 None:intergenic 25.0%
! ACTAACACTAATGTTATTCA+TGG + Chr8:75319895-75319914 MsG0880046598.01.T01:CDS 25.0%
! ATCAATCGATCAGTTATAAA+TGG - Chr8:75323186-75323205 None:intergenic 25.0%
! ATCTGTTCAAATCAAATGAT+GGG - Chr8:75319797-75319816 None:intergenic 25.0%
! ATCTTCACATACTTAACAAA+GGG - Chr8:75320205-75320224 None:intergenic 25.0%
! GAAAAAAAATTGCATACACA+AGG - Chr8:75320635-75320654 None:intergenic 25.0%
! GAACTCTATATATTAGGAAT+TGG + Chr8:75321991-75322010 MsG0880046598.01.T01:intron 25.0%
! GCATTAAAAGAATATCATGA+TGG - Chr8:75321661-75321680 None:intergenic 25.0%
! GCTAAAATAAAAGTTACCTA+AGG - Chr8:75322607-75322626 None:intergenic 25.0%
! GTAACTAGTGTATATATAGA+GGG + Chr8:75322105-75322124 MsG0880046598.01.T01:intron 25.0%
! TATCTGTTCAAATCAAATGA+TGG - Chr8:75319798-75319817 None:intergenic 25.0%
! TCAATCGATCAGTTATAAAT+GGG - Chr8:75323185-75323204 None:intergenic 25.0%
! TCAGTTATAAATGGGAAAAA+TGG - Chr8:75323177-75323196 None:intergenic 25.0%
! TGTAACTAGTGTATATATAG+AGG + Chr8:75322104-75322123 MsG0880046598.01.T01:intron 25.0%
!! ATGTTTGGTTTCATATTTAG+AGG + Chr8:75322647-75322666 MsG0880046598.01.T01:intron 25.0%
!! CTATCAAGTTTTTGCTTTAT+CGG + Chr8:75321919-75321938 MsG0880046598.01.T01:intron 25.0%
!! CTTCAAAGTTTAGAATTGAA+GGG - Chr8:75322500-75322519 None:intergenic 25.0%
!! CTTTTTAACATTTAAAAGGG+GGG - Chr8:75319585-75319604 None:intergenic 25.0%
!! GCTAACTTTATACAATTTTG+GGG - Chr8:75319623-75319642 None:intergenic 25.0%
!! GTTATTTAACTTTCCTCAAT+CGG - Chr8:75320230-75320249 None:intergenic 25.0%
!! TATTTTCCAAGATACTATTC+GGG - Chr8:75321369-75321388 None:intergenic 25.0%
!! TTAACTAGCTCTTATCATAT+TGG - Chr8:75320526-75320545 None:intergenic 25.0%
!! TTAAGTTGTTTATCGAAACA+GGG + Chr8:75320542-75320561 MsG0880046598.01.T01:intron 25.0%
!! TTCAAAGTTTAGAATTGAAG+GGG - Chr8:75322499-75322518 None:intergenic 25.0%
!! TTCAGCATTATCAAAAGTAT+TGG - Chr8:75322866-75322885 None:intergenic 25.0%
!!! TCAATTTTGAGGATCAAATT+GGG + Chr8:75319723-75319742 MsG0880046598.01.T01:intron 25.0%
!!! TCTTGAGTTGTGAAATATTT+TGG + Chr8:75321032-75321051 MsG0880046598.01.T01:intron 25.0%
!!! TTCAATTTTGAGGATCAAAT+TGG + Chr8:75319722-75319741 MsG0880046598.01.T01:intron 25.0%
!!! TTGAAGTTGATATACATCAT+TGG - Chr8:75319933-75319952 None:intergenic 25.0%
!!! TTTTTTTTTTTGGGTTGTGT+AGG + Chr8:75320682-75320701 MsG0880046598.01.T01:intron 25.0%
AAAATATCCTGGAAATCGTA+TGG - Chr8:75320427-75320446 None:intergenic 30.0%
AAGCAGTAAAAGAATCAAGA+TGG + Chr8:75323037-75323056 MsG0880046598.01.T01:CDS 30.0%
AATAAAAGTTACCTAAGGCA+AGG - Chr8:75322602-75322621 None:intergenic 30.0%
AATCAAAGACTTGCATCAAA+TGG - Chr8:75321203-75321222 None:intergenic 30.0%
ATAAAAGTTACCTAAGGCAA+GGG - Chr8:75322601-75322620 None:intergenic 30.0%
CAAGAAAGAAACAATTCTAG+AGG - Chr8:75322710-75322729 None:intergenic 30.0%
CATTCCAATATAAGATGATC+CGG - Chr8:75322920-75322939 None:intergenic 30.0%
CCATTAGCATTATTCACATA+GGG - Chr8:75320081-75320100 None:intergenic 30.0%
CCTTCAATTCTAAACTTTGA+AGG + Chr8:75322498-75322517 MsG0880046598.01.T01:intron 30.0%
CTAACTCTAAGTTAGTGAAT+AGG + Chr8:75322031-75322050 MsG0880046598.01.T01:intron 30.0%
GACTAAAAATCAGCTATAGA+GGG - Chr8:75322754-75322773 None:intergenic 30.0%
GGGTATGTTTGGATAATAAT+TGG - Chr8:75320061-75320080 None:intergenic 30.0%
GTATAAAGTTAGCAATGTCA+TGG + Chr8:75319630-75319649 MsG0880046598.01.T01:intron 30.0%
GTTACAACTAAGCATATTGA+AGG + Chr8:75322561-75322580 MsG0880046598.01.T01:CDS 30.0%
TAGTATGCTATACAAAGCAA+AGG - Chr8:75322396-75322415 None:intergenic 30.0%
TATGTGAATAATGCTAATGG+AGG + Chr8:75320081-75320100 MsG0880046598.01.T01:intron 30.0%
TCCATTAGCATTATTCACAT+AGG - Chr8:75320082-75320101 None:intergenic 30.0%
TTAATAGACATTCTGTTTGG+TGG + Chr8:75320285-75320304 MsG0880046598.01.T01:intron 30.0%
TTACTTGGACAAATTCGTAA+CGG + Chr8:75321438-75321457 MsG0880046598.01.T01:CDS 30.0%
TTATTCACATAGGGTATGTT+TGG - Chr8:75320072-75320091 None:intergenic 30.0%
TTTGAAGGCCATAAAAGAAA+AGG - Chr8:75323100-75323119 None:intergenic 30.0%
TTTGCTTTGTATAGCATACT+AGG + Chr8:75322395-75322414 MsG0880046598.01.T01:intron 30.0%
TTTGTATAGCATACTAGGTT+CGG + Chr8:75322400-75322419 MsG0880046598.01.T01:intron 30.0%
! AATTGCGTTGGTAAATTACT+TGG + Chr8:75321423-75321442 MsG0880046598.01.T01:CDS 30.0%
! ATGCAGTTATCAGAGTTTTT+AGG + Chr8:75319988-75320007 MsG0880046598.01.T01:intron 30.0%
! CCTTCAAAGTTTAGAATTGA+AGG - Chr8:75322501-75322520 None:intergenic 30.0%
! CTATGTGTCCTTTTCTTTTA+TGG + Chr8:75323089-75323108 MsG0880046598.01.T01:three_prime_UTR 30.0%
! GATACACTACTTCAATTTTG+AGG + Chr8:75319712-75319731 MsG0880046598.01.T01:intron 30.0%
! GCTAACATTTTACGTAGAAA+TGG - Chr8:75319959-75319978 None:intergenic 30.0%
! GTTAAGTTGTTTATCGAAAC+AGG + Chr8:75320541-75320560 MsG0880046598.01.T01:intron 30.0%
! TCTTATTTGAGCTTATCTAC+TGG + Chr8:75320322-75320341 MsG0880046598.01.T01:intron 30.0%
! TTTTCTTTTATGGCCTTCAA+AGG + Chr8:75323099-75323118 MsG0880046598.01.T01:three_prime_UTR 30.0%
!! AAAAGTATTCATTTGGCATG+TGG - Chr8:75322204-75322223 None:intergenic 30.0%
!! ACTTTATACAATTTTGGGGA+TGG - Chr8:75319619-75319638 None:intergenic 30.0%
!! TTAACAAAGGGAGTATTTCT+AGG - Chr8:75320193-75320212 None:intergenic 30.0%
!! TTTTAACTAGTTACAACCCA+AGG - Chr8:75321575-75321594 None:intergenic 30.0%
!!! TACTTTTGATAATGCTGAAC+AGG + Chr8:75322867-75322886 MsG0880046598.01.T01:intron 30.0%
ACTTATGAGACTGTTTGAGA+GGG + Chr8:75320352-75320371 MsG0880046598.01.T01:intron 35.0%
AGATACTGAAGGAAGCTTTA+TGG + Chr8:75321246-75321265 MsG0880046598.01.T01:intron 35.0%
AGTGTATATATAGAGGGATG+AGG + Chr8:75322111-75322130 MsG0880046598.01.T01:intron 35.0%
AGTTTAATGCACCCAATAGA+CGG - Chr8:75321970-75321989 None:intergenic 35.0%
ATCTAGATCAAGACAAGAAG+TGG - Chr8:75320761-75320780 None:intergenic 35.0%
CACAAAAGTTCACTATACTG+AGG - Chr8:75323143-75323162 None:intergenic 35.0%
CATCTTACTTCGTGTATGTT+TGG + Chr8:75322632-75322651 MsG0880046598.01.T01:intron 35.0%
CCCTATGTGAATAATGCTAA+TGG + Chr8:75320078-75320097 MsG0880046598.01.T01:intron 35.0%
CGGATCATCTTATATTGGAA+TGG + Chr8:75322918-75322937 MsG0880046598.01.T01:CDS 35.0%
CTAAAACAATTGCCTGAATG+CGG - Chr8:75321623-75321642 None:intergenic 35.0%
CTATACTGAACAAATTGCGT+TGG + Chr8:75321411-75321430 MsG0880046598.01.T01:CDS 35.0%
CTATATCTACATCCCATCTA+GGG - Chr8:75320161-75320180 None:intergenic 35.0%
CTTGATCTAGATAACATCCT+TGG + Chr8:75320769-75320788 MsG0880046598.01.T01:CDS 35.0%
GCTTGCAAAAAACTTCTCTT+TGG + Chr8:75321798-75321817 MsG0880046598.01.T01:CDS 35.0%
GGACTAAAAATCAGCTATAG+AGG - Chr8:75322755-75322774 None:intergenic 35.0%
TATGGAGTCAAGCTAAAACA+AGG - Chr8:75320409-75320428 None:intergenic 35.0%
TATTTATTTAGGTGGAGTCC+CGG + Chr8:75320939-75320958 MsG0880046598.01.T01:intron 35.0%
TCACTAACTTAGAGTTAGAG+TGG - Chr8:75322029-75322048 None:intergenic 35.0%
TCTATATCTACATCCCATCT+AGG - Chr8:75320162-75320181 None:intergenic 35.0%
TGCAAAGAAGTGACTGAAAT+TGG + Chr8:75320805-75320824 MsG0880046598.01.T01:CDS 35.0%
TTTGTCCAAATCACTACTTC+TGG - Chr8:75319866-75319885 None:intergenic 35.0%
! AATTTCTGAGAGTGAAGCTT+TGG + Chr8:75319822-75319841 MsG0880046598.01.T01:CDS 35.0%
! AGTGATTTGGACAAATCAAG+TGG + Chr8:75319871-75319890 MsG0880046598.01.T01:CDS 35.0%
! GCATCGGTAAAACACAAATA+GGG + Chr8:75320962-75320981 MsG0880046598.01.T01:CDS 35.0%
! GTGATTTGGACAAATCAAGT+GGG + Chr8:75319872-75319891 MsG0880046598.01.T01:CDS 35.0%
! TATGTGAAGATTACCGATTG+AGG + Chr8:75320214-75320233 MsG0880046598.01.T01:intron 35.0%
! TATTTGATAGCAGTGACATG+AGG + Chr8:75321874-75321893 MsG0880046598.01.T01:intron 35.0%
!!! GCATTTTAACTCCAAAAGCT+TGG - Chr8:75321345-75321364 None:intergenic 35.0%
!!! TTTTTTTTGGGTTGTGTAGG+TGG + Chr8:75320685-75320704 MsG0880046598.01.T01:intron 35.0%
AATCGGAATGCTACCAATCT+CGG + Chr8:75319449-75319468 MsG0880046598.01.T01:CDS 40.0%
AATGCACCCGAATAGTATCT+TGG + Chr8:75321360-75321379 MsG0880046598.01.T01:intron 40.0%
ACCATACTCTGTGACTTCTA+GGG + Chr8:75322995-75323014 MsG0880046598.01.T01:CDS 40.0%
AGACTGTTTGAGAGGGTTTA+TGG + Chr8:75320359-75320378 MsG0880046598.01.T01:intron 40.0%
ATTGGTGAGTGACTGTTTGT+TGG + Chr8:75320823-75320842 MsG0880046598.01.T01:intron 40.0%
ATTTATTTAGGTGGAGTCCC+GGG + Chr8:75320940-75320959 MsG0880046598.01.T01:intron 40.0%
CAAAAGCCAGTATCCTTTGA+AGG - Chr8:75323115-75323134 None:intergenic 40.0%
CACTTATGAGACTGTTTGAG+AGG + Chr8:75320351-75320370 MsG0880046598.01.T01:intron 40.0%
CATATTTAGAGGAGACAGAG+AGG + Chr8:75322658-75322677 MsG0880046598.01.T01:intron 40.0%
CCATATCATCAAAGTCTTGG+CGG - Chr8:75321732-75321751 None:intergenic 40.0%
CGAACCGGATCATCTTATAT+TGG + Chr8:75322913-75322932 MsG0880046598.01.T01:CDS 40.0%
CTATACAAAGCAAAGGCAGT+TGG - Chr8:75322389-75322408 None:intergenic 40.0%
GAGCCATATCATCAAAGTCT+TGG - Chr8:75321735-75321754 None:intergenic 40.0%
GATCTAGATAACATCCTTGG+TGG + Chr8:75320772-75320791 MsG0880046598.01.T01:CDS 40.0%
GCATGATACTCAGATACTGA+AGG + Chr8:75321235-75321254 MsG0880046598.01.T01:intron 40.0%
GGAAAAGTAACGAAAGAGAG+TGG + Chr8:75319416-75319435 MsG0880046598.01.T01:five_prime_UTR 40.0%
GTTCAGATTCCCTTAGACTA+TGG + Chr8:75321109-75321128 MsG0880046598.01.T01:CDS 40.0%
TAGATAACATCCTTGGTGGA+GGG + Chr8:75320776-75320795 MsG0880046598.01.T01:CDS 40.0%
TGAAAATGAAACCTCGAGCA+AGG - Chr8:75319548-75319567 None:intergenic 40.0%
TGCAACCAGAAGTAGTGATT+TGG + Chr8:75319858-75319877 MsG0880046598.01.T01:CDS 40.0%
TGCACAATTCAGACAAGCAA+AGG - Chr8:75322468-75322487 None:intergenic 40.0%
TTATGGCCTTCAAAGGATAC+TGG + Chr8:75323106-75323125 MsG0880046598.01.T01:three_prime_UTR 40.0%
TTCTCACTGTACAATGCCTT+GGG + Chr8:75321556-75321575 MsG0880046598.01.T01:intron 40.0%
TTTCAATTGACCCTTGCCTT+AGG + Chr8:75322588-75322607 MsG0880046598.01.T01:CDS 40.0%
TTTCACCAAGTAATCGAGTC+CGG - Chr8:75321759-75321778 None:intergenic 40.0%
! ACTTTGATGATATGGCTCTC+CGG + Chr8:75321737-75321756 MsG0880046598.01.T01:CDS 40.0%
! ATTTGTGTTTTACCGATGCC+CGG - Chr8:75320961-75320980 None:intergenic 40.0%
! CACAAGGATAACCAAGCTTT+TGG + Chr8:75321331-75321350 MsG0880046598.01.T01:intron 40.0%
! GCTGAAGCTGATTTAAGAGA+AGG - Chr8:75322975-75322994 None:intergenic 40.0%
! GCTGACTTCACAAAATATCC+TGG - Chr8:75320438-75320457 None:intergenic 40.0%
! TTCTGTTTGGTGGACATGTT+TGG + Chr8:75320295-75320314 MsG0880046598.01.T01:intron 40.0%
!! GATGGTGTGAGATTTTGAGT+AGG + Chr8:75323055-75323074 MsG0880046598.01.T01:exon 40.0%
!! GCTTATTTTCTTCCGCATTC+AGG + Chr8:75321608-75321627 MsG0880046598.01.T01:intron 40.0%
!! GGCATCGGTAAAACACAAAT+AGG + Chr8:75320961-75320980 MsG0880046598.01.T01:CDS 40.0%
AATAGACGGAATCAGCGGTA+CGG - Chr8:75321956-75321975 None:intergenic 45.0%
AATGCTGAACAGGTGATAGC+TGG + Chr8:75322877-75322896 MsG0880046598.01.T01:intron 45.0%
AGAATAGCCACCACTTCACA+AGG + Chr8:75321315-75321334 MsG0880046598.01.T01:intron 45.0%
AGAGTGGTCGACATGGAAAT+CGG + Chr8:75319432-75319451 MsG0880046598.01.T01:exon 45.0%
CACCATACTCTGTGACTTCT+AGG + Chr8:75322994-75323013 MsG0880046598.01.T01:CDS 45.0%
CAGATTCCCTTAGACTATGG+TGG + Chr8:75321112-75321131 MsG0880046598.01.T01:CDS 45.0%
CATACTCTGTGACTTCTAGG+GGG + Chr8:75322997-75323016 MsG0880046598.01.T01:CDS 45.0%
CCATACTCTGTGACTTCTAG+GGG + Chr8:75322996-75323015 MsG0880046598.01.T01:CDS 45.0%
CTAGATAACATCCTTGGTGG+AGG + Chr8:75320775-75320794 MsG0880046598.01.T01:CDS 45.0%
CTTCTCACTGTACAATGCCT+TGG + Chr8:75321555-75321574 MsG0880046598.01.T01:intron 45.0%
CTTGACTCCATACGATTTCC+AGG + Chr8:75320417-75320436 MsG0880046598.01.T01:intron 45.0%
GAAACAGGGTCTCTTACCAT+TGG + Chr8:75320556-75320575 MsG0880046598.01.T01:intron 45.0%
GCTTGGTTATCCTTGTGAAG+TGG - Chr8:75321328-75321347 None:intergenic 45.0%
GGTGGGAAGGCAATATACAT+CGG + Chr8:75321139-75321158 MsG0880046598.01.T01:CDS 45.0%
GGTTAAGCACGAGAATCGAT+TGG - Chr8:75320140-75320159 None:intergenic 45.0%
TAGAGTTAGAGTGGCTATGC+AGG - Chr8:75322020-75322039 None:intergenic 45.0%
TCTCGTGCTTAACCCTAGAT+GGG + Chr8:75320146-75320165 MsG0880046598.01.T01:intron 45.0%
TGCAATTAATCCCTCCACCA+AGG - Chr8:75320789-75320808 None:intergenic 45.0%
TGCTTGGGATATGCTCAATG+AGG + Chr8:75320714-75320733 MsG0880046598.01.T01:CDS 45.0%
TGGTTATCCTTGTGAAGTGG+TGG - Chr8:75321325-75321344 None:intergenic 45.0%
TGTACTTCCACATCTTGACC+TGG + Chr8:75322137-75322156 MsG0880046598.01.T01:intron 45.0%
TGTATATTGCCTTCCCACCT+AGG - Chr8:75321138-75321157 None:intergenic 45.0%
TTCTCGTGCTTAACCCTAGA+TGG + Chr8:75320145-75320164 MsG0880046598.01.T01:intron 45.0%
TTTGGCATGTGGCATTGCTT+AGG - Chr8:75322193-75322212 None:intergenic 45.0%
TTTGTCATTGAGCACACGCA+TGG - Chr8:75320264-75320283 None:intergenic 45.0%
! AGGTCAAGATGTGGAAGTAC+AGG - Chr8:75322138-75322157 None:intergenic 45.0%
! CCGCCAAGACTTTGATGATA+TGG + Chr8:75321729-75321748 MsG0880046598.01.T01:CDS 45.0%
! TAGAGACTAGAGTGTCCACT+TGG - Chr8:75322426-75322445 None:intergenic 45.0%
! TTTGTGTTTTACCGATGCCC+GGG - Chr8:75320960-75320979 None:intergenic 45.0%
!! CAAGGCATTGTACAGTGAGA+AGG - Chr8:75321557-75321576 None:intergenic 45.0%
!! TACCGCTGATTCCGTCTATT+GGG + Chr8:75321956-75321975 MsG0880046598.01.T01:intron 45.0%
ACAACCACCAGGTCAAGATG+TGG - Chr8:75322147-75322166 None:intergenic 50.0%
ACATCTTGACCTGGTGGTTG+TGG + Chr8:75322146-75322165 MsG0880046598.01.T01:intron 50.0%
ACCTAGGCCACCATAGTCTA+AGG - Chr8:75321122-75321141 None:intergenic 50.0%
ACGAAAGAGAGTGGTCGACA+TGG + Chr8:75319425-75319444 MsG0880046598.01.T01:exon 50.0%
ACTTCCACATCTTGACCTGG+TGG + Chr8:75322140-75322159 MsG0880046598.01.T01:intron 50.0%
AGAGGAAAGCTCTTAGCAGC+TGG + Chr8:75319480-75319499 MsG0880046598.01.T01:CDS 50.0%
CACCCAATAGACGGAATCAG+CGG - Chr8:75321961-75321980 None:intergenic 50.0%
CATCAAAGTCTTGGCGGAAG+TGG - Chr8:75321726-75321745 None:intergenic 50.0%
CCAATCTCGGCATCAAAGAG+AGG + Chr8:75319462-75319481 MsG0880046598.01.T01:CDS 50.0%
CCCCTAGAAGTCACAGAGTA+TGG - Chr8:75322999-75323018 None:intergenic 50.0%
CCTAGGCCACCATAGTCTAA+GGG - Chr8:75321121-75321140 None:intergenic 50.0%
CCTCTCTTTGATGCCGAGAT+TGG - Chr8:75319465-75319484 None:intergenic 50.0%
GGCATTGCTTAGGTGGATGA+CGG - Chr8:75322183-75322202 None:intergenic 50.0%
TAGACTATGGTGGCCTAGGT+GGG + Chr8:75321122-75321141 MsG0880046598.01.T01:CDS 50.0%
TCACAGATTCCACAACCACC+AGG - Chr8:75322158-75322177 None:intergenic 50.0%
TGCGTAGTGTTTGCTTCTGC+AGG + Chr8:75321068-75321087 MsG0880046598.01.T01:intron 50.0%
TGTAGGTGGTCAGACTGCTT+GGG + Chr8:75320699-75320718 MsG0880046598.01.T01:intron 50.0%
TTAGACTATGGTGGCCTAGG+TGG + Chr8:75321121-75321140 MsG0880046598.01.T01:CDS 50.0%
! AGTGGTGGAAGTGCGAAGAA+TGG - Chr8:75319523-75319542 None:intergenic 50.0%
! TGGAAGTGCGAAGAATGGAG+TGG - Chr8:75319518-75319537 None:intergenic 50.0%
!! GTACCGCTGATTCCGTCTAT+TGG + Chr8:75321955-75321974 MsG0880046598.01.T01:intron 50.0%
CCCTTAGACTATGGTGGCCT+AGG + Chr8:75321118-75321137 MsG0880046598.01.T01:CDS 55.0%
GAAACCTCGAGCAAGGTGAG+TGG - Chr8:75319541-75319560 None:intergenic 55.0%
GCATACTAGGTTCGGCCAAG+TGG + Chr8:75322408-75322427 MsG0880046598.01.T01:intron 55.0%
GCTCTCCGGACTCGATTACT+TGG + Chr8:75321751-75321770 MsG0880046598.01.T01:CDS 55.0%
GGCATGTGGCATTGCTTAGG+TGG - Chr8:75322190-75322209 None:intergenic 55.0%
GTGTAGGTGGTCAGACTGCT+TGG + Chr8:75320698-75320717 MsG0880046598.01.T01:intron 55.0%
ACCTCGAGCAAGGTGAGTGG+TGG - Chr8:75319538-75319557 None:intergenic 60.0%
CTATGGTGGCCTAGGTGGGA+AGG + Chr8:75321126-75321145 MsG0880046598.01.T01:CDS 60.0%
GGTCGCATTCGTGCACGAAC+CGG + Chr8:75322898-75322917 MsG0880046598.01.T01:CDS 60.0%
TCCACCACTCACCTTGCTCG+AGG + Chr8:75319534-75319553 MsG0880046598.01.T01:CDS 60.0%
TTAGGTGGAGTCCCGGGCAT+CGG + Chr8:75320946-75320965 MsG0880046598.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr8 gene 75319414 75323236 75319414 ID=MsG0880046598.01;Name=MsG0880046598.01
Chr8 mRNA 75319414 75323236 75319414 ID=MsG0880046598.01.T01;Parent=MsG0880046598.01;Name=MsG0880046598.01.T01;_AED=0.50;_eAED=0.50;_QI=30|0.88|0.8|1|0.88|0.9|10|172|313
Chr8 exon 75319414 75319555 75319414 ID=MsG0880046598.01.T01:exon:31581;Parent=MsG0880046598.01.T01
Chr8 exon 75319815 75319916 75319815 ID=MsG0880046598.01.T01:exon:31582;Parent=MsG0880046598.01.T01
Chr8 exon 75320704 75320826 75320704 ID=MsG0880046598.01.T01:exon:31583;Parent=MsG0880046598.01.T01
Chr8 exon 75320950 75320983 75320950 ID=MsG0880046598.01.T01:exon:31584;Parent=MsG0880046598.01.T01
Chr8 exon 75321090 75321160 75321090 ID=MsG0880046598.01.T01:exon:31585;Parent=MsG0880046598.01.T01
Chr8 exon 75321248 75321311 75321248 ID=MsG0880046598.01.T01:exon:31586;Parent=MsG0880046598.01.T01
Chr8 exon 75321411 75321471 75321411 ID=MsG0880046598.01.T01:exon:31587;Parent=MsG0880046598.01.T01
Chr8 exon 75321691 75321822 75321691 ID=MsG0880046598.01.T01:exon:31588;Parent=MsG0880046598.01.T01
Chr8 exon 75322543 75322609 75322543 ID=MsG0880046598.01.T01:exon:31589;Parent=MsG0880046598.01.T01
Chr8 exon 75322889 75323236 75322889 ID=MsG0880046598.01.T01:exon:31590;Parent=MsG0880046598.01.T01
Chr8 five_prime_UTR 75319414 75319443 75319414 ID=MsG0880046598.01.T01:five_prime_utr;Parent=MsG0880046598.01.T01
Chr8 CDS 75319444 75319555 75319444 ID=MsG0880046598.01.T01:cds;Parent=MsG0880046598.01.T01
Chr8 CDS 75319815 75319916 75319815 ID=MsG0880046598.01.T01:cds;Parent=MsG0880046598.01.T01
Chr8 CDS 75320704 75320826 75320704 ID=MsG0880046598.01.T01:cds;Parent=MsG0880046598.01.T01
Chr8 CDS 75320950 75320983 75320950 ID=MsG0880046598.01.T01:cds;Parent=MsG0880046598.01.T01
Chr8 CDS 75321090 75321160 75321090 ID=MsG0880046598.01.T01:cds;Parent=MsG0880046598.01.T01
Chr8 CDS 75321248 75321311 75321248 ID=MsG0880046598.01.T01:cds;Parent=MsG0880046598.01.T01
Chr8 CDS 75321411 75321471 75321411 ID=MsG0880046598.01.T01:cds;Parent=MsG0880046598.01.T01
Chr8 CDS 75321691 75321822 75321691 ID=MsG0880046598.01.T01:cds;Parent=MsG0880046598.01.T01
Chr8 CDS 75322543 75322609 75322543 ID=MsG0880046598.01.T01:cds;Parent=MsG0880046598.01.T01
Chr8 CDS 75322889 75323064 75322889 ID=MsG0880046598.01.T01:cds;Parent=MsG0880046598.01.T01
Chr8 three_prime_UTR 75323065 75323236 75323065 ID=MsG0880046598.01.T01:three_prime_utr;Parent=MsG0880046598.01.T01
Gene Sequence

>MsG0880046598.01.T01

ATGGAAATCGGAATGCTACCAATCTCGGCATCAAAGAGAGGAAAGCTCTTAGCAGCTGGTTACACAACACTCCACTCCATTCTTCGCACTTCCACCACTCACCTTGCTCGAGATATACAAATTTCTGAGAGTGAAGCTTTGGAGATTCTGAATTTTGCAACCAGAAGTAGTGATTTGGACAAATCAAGTGGGACTAACACTAATGTTATTCATGGTGGTCAGACTGCTTGGGATATGCTCAATGAGGAAAGATTTTCTTCTCTCATTACCACTTCTTGTCTTGATCTAGATAACATCCTTGGTGGAGGGATTAATTGCAAAGAAGTGACTGAAATTGGTGGAGTCCCGGGCATCGGTAAAACACAAATAGGGATTCAACTTGCAGTAAATGTTCAGATTCCCTTAGACTATGGTGGCCTAGGTGGGAAGGCAATATACATCGATACTGAAGGAAGCTTTATGGTTGAACGTGTTCTTCAAATTGCTGAAGCATGCATAGAAGACATCTATACTGAACAAATTGCGTTGGTAAATTACTTGGACAAATTCGTAACGGAGCATAAAGATGTGAAGATCATCATTATTGATAGTGTTACTTTCCACTTCCGCCAAGACTTTGATGATATGGCTCTCCGGACTCGATTACTTGGTGAAATGTCATTGAAGTTGATGAAGCTTGCAAAAAACTTCTCTTTGGCTGTTGTTATGTTGAATCAAGTTACAACTAAGCATATTGAAGGTTCATTTCAATTGACCCTTGCCTTAGGTGATAGCTGGTCGCATTCGTGCACGAACCGGATCATCTTATATTGGAATGGCGATGAAAGACATGCATACATTGACAAGTCTCCTTCTCTTAAATCAGCTTCAGCACCATACTCTGTGACTTCTAGGGGGATACGTAATTCTACTTCAAGCAGTAAAAGAATCAAGATGGTGTGA
Protein sequence

>MsG0880046598.01.T01

MEIGMLPISASKRGKLLAAGYTTLHSILRTSTTHLARDIQISESEALEILNFATRSSDLDKSSGTNTNVIHGGQTAWDMLNEERFSSLITTSCLDLDNILGGGINCKEVTEIGGVPGIGKTQIGIQLAVNVQIPLDYGGLGGKAIYIDTEGSFMVERVLQIAEACIEDIYTEQIALVNYLDKFVTEHKDVKIIIIDSVTFHFRQDFDDMALRTRLLGEMSLKLMKLAKNFSLAVVMLNQVTTKHIEGSFQLTLALGDSWSHSCTNRIILYWNGDERHAYIDKSPSLKSASAPYSVTSRGIRNSTSSSKRIKMV*