Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047519.01.T01 | XP_003610198.1 | 94.398 | 482 | 17 | 2 | 1 | 472 | 1 | 482 | 0 | 922 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048016.01 | MsG0880047519.01 | 0.841398 | 4.868842e-58 | 7.145606e-55 |
| MsG0080048307.01 | MsG0880047519.01 | 0.825359 | 4.894470e-54 | 4.451073e-51 |
| MsG0080048845.01 | MsG0880047519.01 | 0.834554 | 2.802803e-56 | 3.337950e-53 |
| MsG0080048912.01 | MsG0880047519.01 | 0.802575 | 5.257483e-49 | 2.584307e-46 |
| MsG0080049040.01 | MsG0880047519.01 | 0.803271 | 3.774375e-49 | 1.888578e-46 |
| MsG0080049068.01 | MsG0880047519.01 | 0.804993 | 1.654055e-49 | 8.651489e-47 |
| MsG0080049130.01 | MsG0880047519.01 | 0.815818 | 7.607922e-52 | 5.300099e-49 |
| MsG0180000114.01 | MsG0880047519.01 | 0.861584 | 9.256990e-64 | 2.632393e-60 |
| MsG0180000218.01 | MsG0880047519.01 | -0.813647 | 2.302527e-51 | 1.512190e-48 |
| MsG0180000482.01 | MsG0880047519.01 | 0.803796 | 2.938041e-49 | 1.490083e-46 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047519.01.T01 | MTR_4g129070 | 94.398 | 482 | 17 | 2 | 1 | 472 | 1 | 482 | 0.0 | 922 |
| MsG0880047519.01.T01 | MTR_4g129070 | 93.721 | 430 | 17 | 2 | 1 | 420 | 1 | 430 | 0.0 | 816 |
| MsG0880047519.01.T01 | MTR_0026s0160 | 73.036 | 471 | 116 | 3 | 12 | 471 | 19 | 489 | 0.0 | 716 |
| MsG0880047519.01.T01 | MTR_4g129070 | 96.519 | 316 | 11 | 0 | 1 | 316 | 1 | 316 | 0.0 | 626 |
| MsG0880047519.01.T01 | MTR_2g089785 | 34.058 | 414 | 243 | 10 | 67 | 462 | 22 | 423 | 2.34e-70 | 231 |
| MsG0880047519.01.T01 | MTR_2g089785 | 34.058 | 414 | 243 | 10 | 67 | 462 | 22 | 423 | 1.07e-69 | 231 |
| MsG0880047519.01.T01 | MTR_8g464780 | 33.842 | 393 | 234 | 8 | 82 | 457 | 33 | 416 | 4.71e-68 | 226 |
| MsG0880047519.01.T01 | MTR_4g045973 | 32.648 | 389 | 246 | 7 | 81 | 457 | 214 | 598 | 1.31e-64 | 221 |
| MsG0880047519.01.T01 | MTR_8g094940 | 31.793 | 368 | 185 | 13 | 85 | 445 | 333 | 641 | 1.60e-35 | 140 |
| MsG0880047519.01.T01 | MTR_4g109390 | 27.614 | 373 | 195 | 10 | 85 | 447 | 359 | 666 | 2.22e-35 | 140 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047519.01.T01 | AT4G30340 | 60.976 | 492 | 170 | 5 | 1 | 471 | 1 | 491 | 0.0 | 631 |
| MsG0880047519.01.T01 | AT2G18730 | 61.983 | 484 | 167 | 4 | 4 | 470 | 3 | 486 | 0.0 | 622 |
| MsG0880047519.01.T01 | AT5G57690 | 63.274 | 452 | 156 | 2 | 30 | 471 | 35 | 486 | 0.0 | 606 |
| MsG0880047519.01.T01 | AT4G30340 | 56.720 | 372 | 139 | 5 | 1 | 351 | 1 | 371 | 1.77e-151 | 436 |
| MsG0880047519.01.T01 | AT4G28130 | 35.176 | 398 | 226 | 9 | 79 | 459 | 41 | 423 | 1.80e-72 | 236 |
| MsG0880047519.01.T01 | AT2G20900 | 34.343 | 396 | 234 | 9 | 81 | 459 | 36 | 422 | 2.46e-69 | 229 |
| MsG0880047519.01.T01 | AT2G20900 | 34.343 | 396 | 234 | 9 | 81 | 459 | 36 | 422 | 2.46e-69 | 229 |
| MsG0880047519.01.T01 | AT2G20900 | 34.343 | 396 | 234 | 9 | 81 | 459 | 36 | 422 | 4.57e-69 | 229 |
| MsG0880047519.01.T01 | AT2G20900 | 34.343 | 396 | 234 | 9 | 81 | 459 | 36 | 422 | 4.57e-69 | 229 |
| MsG0880047519.01.T01 | AT5G63770 | 32.162 | 370 | 184 | 13 | 85 | 447 | 342 | 651 | 3.01e-40 | 154 |
Find 161 sgRNAs with CRISPR-Local
Find 755 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACAACCATAGGTGAAGT+CGG | 0.613615 | 8:+87462712 | None:intergenic |
| AAACATCCTTTCTGATATTA+GGG | 0.227821 | 8:-87455971 | MsG0880047519.01.T01:CDS |
| AAAGGGTTAGTAGCTCAGAG+TGG | 0.694724 | 8:-87455918 | MsG0880047519.01.T01:CDS |
| AAATAAAACCACTTCCACTT+GGG | 0.511495 | 8:+87463056 | None:intergenic |
| AAATATTTCCAAGCGCCCAT+CGG | 0.600632 | 8:+87454667 | None:intergenic |
| AACAAGTTGCAATTCCTAAA+AGG | 0.323846 | 8:-87455894 | MsG0880047519.01.T01:intron |
| AACATCCTTTCTGATATTAG+GGG | 0.641439 | 8:-87455970 | MsG0880047519.01.T01:CDS |
| AAGGAACAATATGTGTGTGT+GGG | 0.673215 | 8:+87463154 | None:intergenic |
| AAGGGTTAGTAGCTCAGAGT+GGG | 0.612993 | 8:-87455917 | MsG0880047519.01.T01:CDS |
| AAGTGGTCCAATTGCAAACA+AGG | 0.538010 | 8:-87459454 | MsG0880047519.01.T01:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAAAATTGAGGGAGTGAAA+TGG | + | Chr8:87457596-87457615 | None:intergenic | 30.0% | |
| AAAAACAAAATGGCATGCAA+AGG | + | Chr8:87459927-87459946 | None:intergenic | 30.0% | |
| AAAACGTAAAGGATCAAATC+GGG | + | Chr8:87461667-87461686 | None:intergenic | 30.0% | |
| AAAAGCTATTGGTGAAAACT+AGG | + | Chr8:87462140-87462159 | None:intergenic | 30.0% | |
| AAAGCTATTGGTGAAAACTA+GGG | + | Chr8:87462139-87462158 | None:intergenic | 30.0% | |
| AAAGTGAAAACACACATATG+AGG | + | Chr8:87459709-87459728 | None:intergenic | 30.0% | |
| AAATAAAACCACTTCCACTT+GGG | + | Chr8:87452986-87453005 | None:intergenic | 30.0% | |
| AACAAGTTGCAATTCCTAAA+AGG | - | Chr8:87460145-87460164 | MsG0880047519.01.T01:CDS | 30.0% | |
| AACACTTCAAAGAAATCAGT+GGG | - | Chr8:87462207-87462226 | MsG0880047519.01.T01:intron | 30.0% | |
| AACATCCTTTCTGATATTAG+GGG | - | Chr8:87460069-87460088 | MsG0880047519.01.T01:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 87452794 | 87463267 | 87452794 | ID=MsG0880047519.01;Name=MsG0880047519.01 |
| Chr8 | mRNA | 87452794 | 87463267 | 87452794 | ID=MsG0880047519.01.T01;Parent=MsG0880047519.01;Name=MsG0880047519.01.T01;_AED=0.41;_eAED=0.41;_QI=290|0.9|0.90|1|1|1|11|255|472 |
| Chr8 | exon | 87452794 | 87453207 | 87452794 | ID=MsG0880047519.01.T01:exon:34775;Parent=MsG0880047519.01.T01 |
| Chr8 | exon | 87454598 | 87454714 | 87454598 | ID=MsG0880047519.01.T01:exon:34774;Parent=MsG0880047519.01.T01 |
| Chr8 | exon | 87455560 | 87455647 | 87455560 | ID=MsG0880047519.01.T01:exon:34773;Parent=MsG0880047519.01.T01 |
| Chr8 | exon | 87455895 | 87456013 | 87455895 | ID=MsG0880047519.01.T01:exon:34772;Parent=MsG0880047519.01.T01 |
| Chr8 | exon | 87459455 | 87459537 | 87459455 | ID=MsG0880047519.01.T01:exon:34771;Parent=MsG0880047519.01.T01 |
| Chr8 | exon | 87460141 | 87460216 | 87460141 | ID=MsG0880047519.01.T01:exon:34770;Parent=MsG0880047519.01.T01 |
| Chr8 | exon | 87461155 | 87461242 | 87461155 | ID=MsG0880047519.01.T01:exon:34769;Parent=MsG0880047519.01.T01 |
| Chr8 | exon | 87461490 | 87461575 | 87461490 | ID=MsG0880047519.01.T01:exon:34768;Parent=MsG0880047519.01.T01 |
| Chr8 | exon | 87461657 | 87461794 | 87461657 | ID=MsG0880047519.01.T01:exon:34767;Parent=MsG0880047519.01.T01 |
| Chr8 | exon | 87462272 | 87462394 | 87462272 | ID=MsG0880047519.01.T01:exon:34766;Parent=MsG0880047519.01.T01 |
| Chr8 | exon | 87462636 | 87463267 | 87462636 | ID=MsG0880047519.01.T01:exon:34765;Parent=MsG0880047519.01.T01 |
| Chr8 | five_prime_UTR | 87462978 | 87463267 | 87462978 | ID=MsG0880047519.01.T01:five_prime_utr;Parent=MsG0880047519.01.T01 |
| Chr8 | CDS | 87462636 | 87462977 | 87462636 | ID=MsG0880047519.01.T01:cds;Parent=MsG0880047519.01.T01 |
| Chr8 | CDS | 87462272 | 87462394 | 87462272 | ID=MsG0880047519.01.T01:cds;Parent=MsG0880047519.01.T01 |
| Chr8 | CDS | 87461657 | 87461794 | 87461657 | ID=MsG0880047519.01.T01:cds;Parent=MsG0880047519.01.T01 |
| Chr8 | CDS | 87461490 | 87461575 | 87461490 | ID=MsG0880047519.01.T01:cds;Parent=MsG0880047519.01.T01 |
| Chr8 | CDS | 87461155 | 87461242 | 87461155 | ID=MsG0880047519.01.T01:cds;Parent=MsG0880047519.01.T01 |
| Chr8 | CDS | 87460141 | 87460216 | 87460141 | ID=MsG0880047519.01.T01:cds;Parent=MsG0880047519.01.T01 |
| Chr8 | CDS | 87459455 | 87459537 | 87459455 | ID=MsG0880047519.01.T01:cds;Parent=MsG0880047519.01.T01 |
| Chr8 | CDS | 87455895 | 87456013 | 87455895 | ID=MsG0880047519.01.T01:cds;Parent=MsG0880047519.01.T01 |
| Chr8 | CDS | 87455560 | 87455647 | 87455560 | ID=MsG0880047519.01.T01:cds;Parent=MsG0880047519.01.T01 |
| Chr8 | CDS | 87454598 | 87454714 | 87454598 | ID=MsG0880047519.01.T01:cds;Parent=MsG0880047519.01.T01 |
| Chr8 | CDS | 87453049 | 87453207 | 87453049 | ID=MsG0880047519.01.T01:cds;Parent=MsG0880047519.01.T01 |
| Chr8 | three_prime_UTR | 87452794 | 87453048 | 87452794 | ID=MsG0880047519.01.T01:three_prime_utr;Parent=MsG0880047519.01.T01 |
| Gene Sequence |
>MsG0880047519.01.T01 ATGGAATCATCGCCATCATCAACGACGGAAGATTCAAAGAAGATACATGTTCGTTCATCACTCGTTCAATCAATTCGCGGCTGCGGTCTCTCCGGTATGCGTATTGACAAAGAAGACTTGAAGAAGCAGCTTACTATGCCACAGTATCTTCGCTTCGCAATGCGTGATTCCATTCGTCTTCAAGATCCTTCCGCCGGCGAAACTCGTTACAGGAATCGCGCTGAAGGAGAAGACTCCGCTGCTCCGACTTCACCTATGGTTGTTTTCATCAATGCTCGTAGCGGCGGCCGTCATGGTCCTGCTCTTAAGGAAAGACTCCAGCAACTCATGAGTGAAGAACAGGTTTTTGATCTTGCTGACGTCAAGCCTCATGAATTTGTATTGTATGGATTGGCTTGTTTGGAGATGTTGGCTGGTCTAGGTGATTCTTGTGCTAAAGAAACTCGTGAAAAACTCAGGGTTATGGTTGCTGGAGGTGATGGTACAGTTGGCTGGGTGTTAGGATGTCTTACAGAACTTCACCAGCTAGGCCGAGAGCCAGTTCCTCCGGTTGGGATCGTACCACTTGGGACAGGAAATGATCTGTCTAGGAGTTTTAACTGGGGTGGTTCATTTCCTTTTGCTTGGAAGTCAGCAATCAAAAGAACTCTTCAAAAGGCTAGTGCTGGGTCAGTTCATCGGTTGGATAGTTGGCGACTTTCCATTTCAATGCCTGAAAGCACAACCGTTAAACCACCATATTGTTTGAAGCCAGCAGAAGAGTTCACTCTTGACCAGGGCATAGAAATTGAGGGAGAACTGTCTGATAAAGTTAAAAGTTATGAAGGCGTGTACTATAATTACTTCAGCATAGGAATGGATGCACAAGTGGCTTATGGCTTCCATCGTCTACGCGATGAAAAACCCTACCTTGCAAGTGGTCCAATTGCAAACAAGAGTATCCTCACTATATTCATAAACATCCTTTCTGATATTAGGGGACTAAGAAATATTTTGCGGATGCATATCAAAAGGGTTAGTAGCTCAGAGTGGGAACAAGTTGCAATTCCTAAAAGTGTAAGGGCAATAGTTGCTTTGAATCTTCATAGCTATGGAAGTGGAAGAAACCCATGGGGTAAACCGAAACCCGAATATTTAGAGAAGAAAGGCTTTGTTGAAGCTGATGTCGCCGATGGGCGCTTGGAAATATTTGGTCTAAAGCAAGGATGGCATGCATCGTTTGTCATGGTTGATTTGATCACTGCGAAGCACATTGCTCAGGCAGCAGCTATTCGATTGGAACTCAGAGGTGGAGGGTGGAAAAATGCCTATTTGCAAATGGATGGAGAACCCTGGAAACAGCCATTAAGCAAGGACTTCTCGACATTCGTGGAGATAAAGAGGGAGCCTTTCCAGTCACTTGTGGTTGATGGATCTTAG |
| Protein sequence |
>MsG0880047519.01.T01 MESSPSSTTEDSKKIHVRSSLVQSIRGCGLSGMRIDKEDLKKQLTMPQYLRFAMRDSIRLQDPSAGETRYRNRAEGEDSAAPTSPMVVFINARSGGRHGPALKERLQQLMSEEQVFDLADVKPHEFVLYGLACLEMLAGLGDSCAKETREKLRVMVAGGDGTVGWVLGCLTELHQLGREPVPPVGIVPLGTGNDLSRSFNWGGSFPFAWKSAIKRTLQKASAGSVHRLDSWRLSISMPESTTVKPPYCLKPAEEFTLDQGIEIEGELSDKVKSYEGVYYNYFSIGMDAQVAYGFHRLRDEKPYLASGPIANKSILTIFINILSDIRGLRNILRMHIKRVSSSEWEQVAIPKSVRAIVALNLHSYGSGRNPWGKPKPEYLEKKGFVEADVADGRLEIFGLKQGWHASFVMVDLITAKHIAQAAAIRLELRGGGWKNAYLQMDGEPWKQPLSKDFSTFVEIKREPFQSLVVDGS* |
