Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0001290 | Msa0055180 | -0.802463 | 5.543952e-49 | -8.615850e-47 |
| Msa0001290 | Msa0233630 | 0.802068 | 6.686920e-49 | -8.615850e-47 |
| Msa0001290 | Msa0265430 | -0.805321 | 1.412447e-49 | -8.615850e-47 |
| Msa0001290 | Msa0340380 | -0.804524 | 2.073074e-49 | -8.615850e-47 |
| Msa0001290 | Msa0661300 | -0.808999 | 2.349710e-50 | -8.615850e-47 |
| Msa0001290 | Msa0981640 | 0.983148 | 5.848758e-157 | -8.615850e-47 |
| Msa0001290 | Msa1165780 | -0.810961 | 8.885960e-51 | -8.615850e-47 |
| Msa0001290 | Msa1261680 | 0.980837 | 3.763278e-151 | -8.615850e-47 |
| Msa0001290 | Msa1296830 | -0.806376 | 8.476664e-50 | -8.615850e-47 |
| Msa0001290 | Msa1431560 | 0.977235 | 2.232630e-143 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0001290 | MtrunA17_Chr4g0063041 | 77.193 | 114 | 11 | 2 | 1 | 99 | 1 | 114 | 5.86e-55 | 167 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 32 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTCATAACCTGAGGGTTTA+AGG | 0.187585 | 1_1:-2980512 | None:intergenic |
| GGAACTTCAATGGGTGCTTC+AGG | 0.232199 | 1_1:+2980400 | Msa0001290:CDS |
| ATCTCCTTTGGAAATTTCAT+TGG | 0.312886 | 1_1:+2980378 | Msa0001290:CDS |
| AAATCCTGTTAAGACCTCTT+TGG | 0.339860 | 1_1:+2980426 | Msa0001290:CDS |
| AATTTCATTGGGAACTTCAA+TGG | 0.362255 | 1_1:+2980390 | Msa0001290:CDS |
| GAAACAGCATCTATCTCCTT+TGG | 0.364301 | 1_1:+2980366 | Msa0001290:CDS |
| TGATTCCAACTGTAAACCTT+TGG | 0.370821 | 1_1:-2980572 | None:intergenic |
| TTCATAACCTGAGGGTTTAA+GGG | 0.376944 | 1_1:-2980511 | None:intergenic |
| AATCCTGTTAAGACCTCTTT+GGG | 0.394298 | 1_1:+2980427 | Msa0001290:CDS |
| ACTCCCAAAGAGGTCTTAAC+AGG | 0.395727 | 1_1:-2980430 | None:intergenic |
| TCTCCTTTGGAAATTTCATT+GGG | 0.430691 | 1_1:+2980379 | Msa0001290:CDS |
| AGTATCATTGCGACCAATGA+TGG | 0.448934 | 1_1:+2980283 | Msa0001290:CDS |
| AGACCTCTTTGGGAGTTTCA+AGG | 0.451544 | 1_1:+2980437 | Msa0001290:CDS |
| ATTTCATTGGGAACTTCAAT+GGG | 0.452362 | 1_1:+2980391 | Msa0001290:CDS |
| TAGAGCTACATGTACTCATT+CGG | 0.458255 | 1_1:+2980471 | Msa0001290:CDS |
| GAACTTCAATGGGTGCTTCA+GGG | 0.458319 | 1_1:+2980401 | Msa0001290:CDS |
| AGCTTCTAACTAACTATCAT+AGG | 0.471561 | 1_1:-2980606 | None:intergenic |
| GGCACTCAAAAGAGCCTGCA+GGG | 0.483137 | 1_1:+2980304 | Msa0001290:CDS |
| CCAATAACCATGAAAGAAAA+CGG | 0.484257 | 1_1:-2980344 | None:intergenic |
| ATGAGTACATGTAGCTCTAA+AGG | 0.492159 | 1_1:-2980467 | None:intergenic |
| GACCTCTTTGGGAGTTTCAA+GGG | 0.493603 | 1_1:+2980438 | Msa0001290:CDS |
| CAGGTCCAAAGGTTTACAGT+TGG | 0.521736 | 1_1:+2980567 | Msa0001290:intron |
| AACTTCAATGGGTGCTTCAG+GGG | 0.554992 | 1_1:+2980402 | Msa0001290:CDS |
| TGGCACTCAAAAGAGCCTGC+AGG | 0.555623 | 1_1:+2980303 | Msa0001290:CDS |
| GTGGAACTTTGCAGGTCCAA+AGG | 0.556653 | 1_1:+2980556 | Msa0001290:intron |
| AGTGCATCCCTTAAACCCTC+AGG | 0.561133 | 1_1:+2980504 | Msa0001290:CDS |
| ACCTCTTTGGGAGTTTCAAG+GGG | 0.579127 | 1_1:+2980439 | Msa0001290:CDS |
| GTTCCCAATGAAATTTCCAA+AGG | 0.582396 | 1_1:-2980382 | None:intergenic |
| GCCCCTTGAAACTCCCAAAG+AGG | 0.583572 | 1_1:-2980440 | None:intergenic |
| ACTTCAATGGGTGCTTCAGG+GGG | 0.595903 | 1_1:+2980403 | Msa0001290:CDS |
| CACTCAAAAGAGCCTGCAGG+GGG | 0.609954 | 1_1:+2980306 | Msa0001290:CDS |
| GCACTCAAAAGAGCCTGCAG+GGG | 0.624648 | 1_1:+2980305 | Msa0001290:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTATGAACAAATCTTGTGTA+AGG | + | chr1_1:2980527-2980546 | Msa0001290:intron | 25.0% |
| CCAATAACCATGAAAGAAAA+CGG | - | chr1_1:2980347-2980366 | None:intergenic | 30.0% | |
| ! | ATCTCCTTTGGAAATTTCAT+TGG | + | chr1_1:2980378-2980397 | Msa0001290:CDS | 30.0% |
| ! | TCTCCTTTGGAAATTTCATT+GGG | + | chr1_1:2980379-2980398 | Msa0001290:CDS | 30.0% |
| AATTTCATTGGGAACTTCAA+TGG | + | chr1_1:2980390-2980409 | Msa0001290:CDS | 30.0% | |
| ATTTCATTGGGAACTTCAAT+GGG | + | chr1_1:2980391-2980410 | Msa0001290:CDS | 30.0% | |
| ! | AAGATTTGTTCATAACCTGA+GGG | - | chr1_1:2980522-2980541 | None:intergenic | 30.0% |
| !! | TGTTTAGCCGTTTTCTTTCA+TGG | + | chr1_1:2980337-2980356 | Msa0001290:CDS | 35.0% |
| ! | CCGTTTTCTTTCATGGTTAT+TGG | + | chr1_1:2980344-2980363 | Msa0001290:CDS | 35.0% |
| GTTCCCAATGAAATTTCCAA+AGG | - | chr1_1:2980385-2980404 | None:intergenic | 35.0% | |
| AAATCCTGTTAAGACCTCTT+TGG | + | chr1_1:2980426-2980445 | Msa0001290:CDS | 35.0% | |
| AATCCTGTTAAGACCTCTTT+GGG | + | chr1_1:2980427-2980446 | Msa0001290:CDS | 35.0% | |
| !! | ATGAGTACATGTAGCTCTAA+AGG | - | chr1_1:2980470-2980489 | None:intergenic | 35.0% |
| TAGAGCTACATGTACTCATT+CGG | + | chr1_1:2980471-2980490 | Msa0001290:CDS | 35.0% | |
| TTCATAACCTGAGGGTTTAA+GGG | - | chr1_1:2980514-2980533 | None:intergenic | 35.0% | |
| ! | CAAGATTTGTTCATAACCTG+AGG | - | chr1_1:2980523-2980542 | None:intergenic | 35.0% |
| !! | ATCTTGTGTAAGGTTTTGTG+TGG | + | chr1_1:2980537-2980556 | Msa0001290:intron | 35.0% |
| TGATTCCAACTGTAAACCTT+TGG | - | chr1_1:2980575-2980594 | None:intergenic | 35.0% | |
| AGTATCATTGCGACCAATGA+TGG | + | chr1_1:2980283-2980302 | Msa0001290:CDS | 40.0% | |
| GAAACAGCATCTATCTCCTT+TGG | + | chr1_1:2980366-2980385 | Msa0001290:CDS | 40.0% | |
| GTTCATAACCTGAGGGTTTA+AGG | - | chr1_1:2980515-2980534 | None:intergenic | 40.0% | |
| !! | GCTCTTTTGAGTGCCATCAT+TGG | - | chr1_1:2980299-2980318 | None:intergenic | 45.0% |
| GAACTTCAATGGGTGCTTCA+GGG | + | chr1_1:2980401-2980420 | Msa0001290:CDS | 45.0% | |
| AACTTCAATGGGTGCTTCAG+GGG | + | chr1_1:2980402-2980421 | Msa0001290:CDS | 45.0% | |
| ACTCCCAAAGAGGTCTTAAC+AGG | - | chr1_1:2980433-2980452 | None:intergenic | 45.0% | |
| ! | AGACCTCTTTGGGAGTTTCA+AGG | + | chr1_1:2980437-2980456 | Msa0001290:CDS | 45.0% |
| ! | GACCTCTTTGGGAGTTTCAA+GGG | + | chr1_1:2980438-2980457 | Msa0001290:CDS | 45.0% |
| ! | ACCTCTTTGGGAGTTTCAAG+GGG | + | chr1_1:2980439-2980458 | Msa0001290:CDS | 45.0% |
| !! | GGTTTTGTGTGGAACTTTGC+AGG | + | chr1_1:2980548-2980567 | Msa0001290:intron | 45.0% |
| CAGGTCCAAAGGTTTACAGT+TGG | + | chr1_1:2980567-2980586 | Msa0001290:intron | 45.0% | |
| GGAACTTCAATGGGTGCTTC+AGG | + | chr1_1:2980400-2980419 | Msa0001290:CDS | 50.0% | |
| ACTTCAATGGGTGCTTCAGG+GGG | + | chr1_1:2980403-2980422 | Msa0001290:CDS | 50.0% | |
| AGTGCATCCCTTAAACCCTC+AGG | + | chr1_1:2980504-2980523 | Msa0001290:CDS | 50.0% | |
| GTGGAACTTTGCAGGTCCAA+AGG | + | chr1_1:2980556-2980575 | Msa0001290:intron | 50.0% | |
| TGGCACTCAAAAGAGCCTGC+AGG | + | chr1_1:2980303-2980322 | Msa0001290:CDS | 55.0% | |
| GGCACTCAAAAGAGCCTGCA+GGG | + | chr1_1:2980304-2980323 | Msa0001290:CDS | 55.0% | |
| GCACTCAAAAGAGCCTGCAG+GGG | + | chr1_1:2980305-2980324 | Msa0001290:CDS | 55.0% | |
| CACTCAAAAGAGCCTGCAGG+GGG | + | chr1_1:2980306-2980325 | Msa0001290:CDS | 55.0% | |
| AACAATCTTCTGCCCCCTGC+AGG | - | chr1_1:2980321-2980340 | None:intergenic | 55.0% | |
| GCCCCTTGAAACTCCCAAAG+AGG | - | chr1_1:2980443-2980462 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_1 | gene | 2980277 | 2980623 | 2980277 | ID=Msa0001290;Name=Msa0001290 |
| chr1_1 | mRNA | 2980277 | 2980623 | 2980277 | ID=Msa0001290-mRNA-1;Parent=Msa0001290;Name=Msa0001290-mRNA-1;_AED=0.43;_eAED=0.53;_QI=0|0|0|1|1|1|2|0|100 |
| chr1_1 | exon | 2980277 | 2980525 | 2980277 | ID=Msa0001290-mRNA-1:exon:640;Parent=Msa0001290-mRNA-1 |
| chr1_1 | exon | 2980570 | 2980623 | 2980570 | ID=Msa0001290-mRNA-1:exon:641;Parent=Msa0001290-mRNA-1 |
| chr1_1 | CDS | 2980277 | 2980525 | 2980277 | ID=Msa0001290-mRNA-1:cds;Parent=Msa0001290-mRNA-1 |
| chr1_1 | CDS | 2980570 | 2980623 | 2980570 | ID=Msa0001290-mRNA-1:cds;Parent=Msa0001290-mRNA-1 |
| Gene Sequence |
| Protein sequence |
