Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1261680 | KEH32101.1 | 95.652 | 69 | 3 | 0 | 1 | 69 | 1 | 69 | 7.14e-40 | 138 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1261680 | A0A072URW0 | 95.652 | 69 | 3 | 0 | 1 | 69 | 1 | 69 | 3.41e-40 | 138 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0001290 | Msa1261680 | 0.980837 | 3.763278e-151 | -8.615850e-47 |
| Msa0048180 | Msa1261680 | -0.802612 | 5.166297e-49 | -8.615850e-47 |
| Msa0052300 | Msa1261680 | 0.831521 | 1.592361e-55 | -8.615850e-47 |
| Msa0052350 | Msa1261680 | 0.825830 | 3.785138e-54 | -8.615850e-47 |
| Msa0055180 | Msa1261680 | -0.812754 | 3.615792e-51 | -8.615850e-47 |
| Msa0097360 | Msa1261680 | 0.814817 | 1.269948e-51 | -8.615850e-47 |
| Msa0100740 | Msa1261680 | -0.800861 | 1.181570e-48 | -8.615850e-47 |
| Msa0603570 | Msa1261680 | -0.838873 | 2.220544e-57 | -8.615850e-47 |
| Msa0615580 | Msa1261680 | -0.819379 | 1.198078e-52 | -8.615850e-47 |
| Msa0620890 | Msa1261680 | -0.804424 | 2.174758e-49 | -8.615850e-47 |
| Msa0661300 | Msa1261680 | -0.821110 | 4.805410e-53 | -8.615850e-47 |
| Msa0666230 | Msa1261680 | -0.801177 | 1.018235e-48 | -8.615850e-47 |
| Msa0748170 | Msa1261680 | -0.800734 | 1.254507e-48 | -8.615850e-47 |
| Msa0800370 | Msa1261680 | -0.805790 | 1.125819e-49 | -8.615850e-47 |
| Msa0818770 | Msa1261680 | -0.805020 | 1.633182e-49 | -8.615850e-47 |
| Msa0146070 | Msa1261680 | 0.819367 | 1.205227e-52 | -8.615850e-47 |
| Msa0162130 | Msa1261680 | -0.802859 | 4.593038e-49 | -8.615850e-47 |
| Msa0190120 | Msa1261680 | -0.808171 | 3.531061e-50 | -8.615850e-47 |
| Msa0207670 | Msa1261680 | -0.808367 | 3.206466e-50 | -8.615850e-47 |
| Msa0209350 | Msa1261680 | -0.805484 | 1.305288e-49 | -8.615850e-47 |
| Msa0209500 | Msa1261680 | -0.805027 | 1.627249e-49 | -8.615850e-47 |
| Msa1242920 | Msa1261680 | 0.804023 | 2.635052e-49 | -8.615850e-47 |
| Msa1261680 | Msa1296830 | -0.831832 | 1.334593e-55 | -8.615850e-47 |
| Msa1261680 | Msa1336560 | -0.808325 | 3.274599e-50 | -8.615850e-47 |
| Msa1261680 | Msa1343290 | -0.826486 | 2.642430e-54 | -8.615850e-47 |
| Msa1261680 | Msa1368120 | -0.809229 | 2.098126e-50 | -8.615850e-47 |
| Msa1261680 | Msa1418960 | 0.822519 | 2.268832e-53 | -8.615850e-47 |
| Msa1261680 | Msa1431560 | 0.993005 | 7.844756e-197 | -8.615850e-47 |
| Msa1261680 | Msa1435170 | 0.816627 | 5.016812e-52 | -8.615850e-47 |
| Msa0370160 | Msa1261680 | 0.803110 | 4.075323e-49 | -8.615850e-47 |
| Msa0414800 | Msa1261680 | -0.806507 | 7.954322e-50 | -8.615850e-47 |
| Msa0417610 | Msa1261680 | 0.826963 | 2.032599e-54 | -8.615850e-47 |
| Msa0437690 | Msa1261680 | 0.831143 | 1.972331e-55 | -8.615850e-47 |
| Msa0443390 | Msa1261680 | -0.828080 | 1.096456e-54 | -8.615850e-47 |
| Msa0981640 | Msa1261680 | 0.985961 | 3.192682e-165 | -8.615850e-47 |
| Msa1019240 | Msa1261680 | -0.800647 | 1.306635e-48 | -8.615850e-47 |
| Msa1019260 | Msa1261680 | -0.810939 | 8.981337e-51 | -8.615850e-47 |
| Msa1081930 | Msa1261680 | -0.801853 | 7.399850e-49 | -8.615850e-47 |
| Msa1104210 | Msa1261680 | -0.821112 | 4.801255e-53 | -8.615850e-47 |
| Msa0265430 | Msa1261680 | -0.815786 | 7.731910e-52 | -8.615850e-47 |
| Msa0271890 | Msa1261680 | -0.803134 | 4.029135e-49 | -8.615850e-47 |
| Msa0340380 | Msa1261680 | -0.811849 | 5.697757e-51 | -8.615850e-47 |
| Msa1122310 | Msa1261680 | 0.804901 | 1.728965e-49 | -8.615850e-47 |
| Msa1126830 | Msa1261680 | -0.821003 | 5.085119e-53 | -8.615850e-47 |
| Msa1165780 | Msa1261680 | -0.843167 | 1.654600e-58 | -8.615850e-47 |
| Msa1166870 | Msa1261680 | -0.820950 | 5.230341e-53 | -8.615850e-47 |
| Msa1200320 | Msa1261680 | -0.802382 | 5.760058e-49 | -8.615850e-47 |
| Msa0509950 | Msa1261680 | 0.837321 | 5.569195e-57 | -8.615850e-47 |
| Msa0557480 | Msa1261680 | -0.802561 | 5.291125e-49 | -8.615850e-47 |
| Msa0563260 | Msa1261680 | 0.804910 | 1.721645e-49 | -8.615850e-47 |
| Msa0581110 | Msa1261680 | -0.816818 | 4.544661e-52 | -8.615850e-47 |
| Msa0586520 | Msa1261680 | 0.823254 | 1.529136e-53 | -8.615850e-47 |
| Msa0588260 | Msa1261680 | -0.819076 | 1.403835e-52 | -8.615850e-47 |
| Msa0881270 | Msa1261680 | -0.820739 | 5.849865e-53 | -8.615850e-47 |
| Msa0974950 | Msa1261680 | -0.806570 | 7.714645e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1261680 | MtrunA17_Chr4g0063041 | 95.652 | 69 | 3 | 0 | 1 | 69 | 1 | 69 | 6.57e-44 | 138 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 28 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAACTTCAATGGGTGCTTC+AGG | 0.232199 | 8_2:-64001293 | Msa1261680:CDS |
| ATCTCCTTTGGAAATTTCAT+TGG | 0.312886 | 8_2:-64001315 | Msa1261680:CDS |
| AAATCCTGTTAAGACCTCTT+TGG | 0.339860 | 8_2:-64001267 | Msa1261680:CDS |
| AATTTCATTGGGAACTTCAA+TGG | 0.362255 | 8_2:-64001303 | Msa1261680:CDS |
| GAAACAGCATCTATCTCCTT+TGG | 0.364301 | 8_2:-64001327 | Msa1261680:CDS |
| TGATTCCAACTGTAAACCTT+TGG | 0.370821 | 8_2:+64001121 | None:intergenic |
| AATCCTGTTAAGACCTCTTT+GGG | 0.394298 | 8_2:-64001266 | Msa1261680:CDS |
| ACTCCCAAAGAGGTCTTAAC+AGG | 0.395727 | 8_2:+64001263 | None:intergenic |
| TCTCCTTTGGAAATTTCATT+GGG | 0.430691 | 8_2:-64001314 | Msa1261680:CDS |
| AGTATCATTGCGACCAATGA+TGG | 0.448934 | 8_2:-64001410 | Msa1261680:CDS |
| AGACCTCTTTGGGAGTTTCA+AGG | 0.451544 | 8_2:-64001256 | Msa1261680:CDS |
| ATTTCATTGGGAACTTCAAT+GGG | 0.452362 | 8_2:-64001302 | Msa1261680:CDS |
| GAACTTCAATGGGTGCTTCA+GGG | 0.458319 | 8_2:-64001292 | Msa1261680:CDS |
| AGCTTCTAACTAACTATCAT+AGG | 0.471561 | 8_2:+64001087 | None:intergenic |
| GGCACTCAAAAGAGCCTGCA+GGG | 0.483137 | 8_2:-64001389 | Msa1261680:CDS |
| CCAATAACCATGAAAGAAAA+CGG | 0.484257 | 8_2:+64001349 | None:intergenic |
| ATGAGTACATGTAGCTCTAA+AGG | 0.492159 | 8_2:+64001226 | None:intergenic |
| GACCTCTTTGGGAGTTTCAA+GGG | 0.493603 | 8_2:-64001255 | Msa1261680:CDS |
| CAGGTCCAAAGGTTTACAGT+TGG | 0.521736 | 8_2:-64001126 | Msa1261680:CDS |
| AACTTCAATGGGTGCTTCAG+GGG | 0.554992 | 8_2:-64001291 | Msa1261680:CDS |
| TGGCACTCAAAAGAGCCTGC+AGG | 0.555623 | 8_2:-64001390 | Msa1261680:CDS |
| GTGGAACTTTGCAGGTCCAA+AGG | 0.556653 | 8_2:-64001137 | Msa1261680:intron |
| ACCTCTTTGGGAGTTTCAAG+GGG | 0.579127 | 8_2:-64001254 | Msa1261680:CDS |
| GTTCCCAATGAAATTTCCAA+AGG | 0.582396 | 8_2:+64001311 | None:intergenic |
| GCCCCTTGAAACTCCCAAAG+AGG | 0.583572 | 8_2:+64001253 | None:intergenic |
| ACTTCAATGGGTGCTTCAGG+GGG | 0.595903 | 8_2:-64001290 | Msa1261680:CDS |
| CACTCAAAAGAGCCTGCAGG+GGG | 0.609954 | 8_2:-64001387 | Msa1261680:CDS |
| GCACTCAAAAGAGCCTGCAG+GGG | 0.624648 | 8_2:-64001388 | Msa1261680:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTATGAACAAATCTTGTGTA+AGG | - | chr8_2:64001346-64001365 | Msa1261680:CDS | 25.0% |
| CCAATAACCATGAAAGAAAA+CGG | + | chr8_2:64001166-64001185 | None:intergenic | 30.0% | |
| ! | ATCTCCTTTGGAAATTTCAT+TGG | - | chr8_2:64001197-64001216 | Msa1261680:intron | 30.0% |
| ! | TCTCCTTTGGAAATTTCATT+GGG | - | chr8_2:64001198-64001217 | Msa1261680:intron | 30.0% |
| AATTTCATTGGGAACTTCAA+TGG | - | chr8_2:64001209-64001228 | Msa1261680:intron | 30.0% | |
| ATTTCATTGGGAACTTCAAT+GGG | - | chr8_2:64001210-64001229 | Msa1261680:intron | 30.0% | |
| ! | AAGATTTGTTCATAACCTGA+GGG | + | chr8_2:64001341-64001360 | None:intergenic | 30.0% |
| !! | TGTTTAGCCGTTTTCTTTCA+TGG | - | chr8_2:64001156-64001175 | Msa1261680:intron | 35.0% |
| ! | CCGTTTTCTTTCATGGTTAT+TGG | - | chr8_2:64001163-64001182 | Msa1261680:intron | 35.0% |
| GTTCCCAATGAAATTTCCAA+AGG | + | chr8_2:64001204-64001223 | None:intergenic | 35.0% | |
| AAATCCTGTTAAGACCTCTT+TGG | - | chr8_2:64001245-64001264 | Msa1261680:CDS | 35.0% | |
| AATCCTGTTAAGACCTCTTT+GGG | - | chr8_2:64001246-64001265 | Msa1261680:CDS | 35.0% | |
| !! | ATGAGTACATGTAGCTCTAA+AGG | + | chr8_2:64001289-64001308 | None:intergenic | 35.0% |
| TAGAGCTACATGTACTCATT+CGG | - | chr8_2:64001290-64001309 | Msa1261680:CDS | 35.0% | |
| TTCATAACCTGAGGGTTTAA+GGG | + | chr8_2:64001333-64001352 | None:intergenic | 35.0% | |
| ! | CAAGATTTGTTCATAACCTG+AGG | + | chr8_2:64001342-64001361 | None:intergenic | 35.0% |
| !! | ATCTTGTGTAAGGTTTTGTG+TGG | - | chr8_2:64001356-64001375 | Msa1261680:CDS | 35.0% |
| TGATTCCAACTGTAAACCTT+TGG | + | chr8_2:64001394-64001413 | None:intergenic | 35.0% | |
| AGTATCATTGCGACCAATGA+TGG | - | chr8_2:64001102-64001121 | Msa1261680:CDS | 40.0% | |
| GAAACAGCATCTATCTCCTT+TGG | - | chr8_2:64001185-64001204 | Msa1261680:intron | 40.0% | |
| GTTCATAACCTGAGGGTTTA+AGG | + | chr8_2:64001334-64001353 | None:intergenic | 40.0% | |
| !! | GCTCTTTTGAGTGCCATCAT+TGG | + | chr8_2:64001118-64001137 | None:intergenic | 45.0% |
| GAACTTCAATGGGTGCTTCA+GGG | - | chr8_2:64001220-64001239 | Msa1261680:intron | 45.0% | |
| AACTTCAATGGGTGCTTCAG+GGG | - | chr8_2:64001221-64001240 | Msa1261680:intron | 45.0% | |
| ACTCCCAAAGAGGTCTTAAC+AGG | + | chr8_2:64001252-64001271 | None:intergenic | 45.0% | |
| ! | AGACCTCTTTGGGAGTTTCA+AGG | - | chr8_2:64001256-64001275 | Msa1261680:CDS | 45.0% |
| ! | GACCTCTTTGGGAGTTTCAA+GGG | - | chr8_2:64001257-64001276 | Msa1261680:CDS | 45.0% |
| ! | ACCTCTTTGGGAGTTTCAAG+GGG | - | chr8_2:64001258-64001277 | Msa1261680:CDS | 45.0% |
| !! | GGTTTTGTGTGGAACTTTGC+AGG | - | chr8_2:64001367-64001386 | Msa1261680:CDS | 45.0% |
| CAGGTCCAAAGGTTTACAGT+TGG | - | chr8_2:64001386-64001405 | Msa1261680:CDS | 45.0% | |
| GGAACTTCAATGGGTGCTTC+AGG | - | chr8_2:64001219-64001238 | Msa1261680:intron | 50.0% | |
| ACTTCAATGGGTGCTTCAGG+GGG | - | chr8_2:64001222-64001241 | Msa1261680:intron | 50.0% | |
| AGTGCATCCCTTAAACCCTC+AGG | - | chr8_2:64001323-64001342 | Msa1261680:CDS | 50.0% | |
| GTGGAACTTTGCAGGTCCAA+AGG | - | chr8_2:64001375-64001394 | Msa1261680:CDS | 50.0% | |
| TGGCACTCAAAAGAGCCTGC+AGG | - | chr8_2:64001122-64001141 | Msa1261680:CDS | 55.0% | |
| GGCACTCAAAAGAGCCTGCA+GGG | - | chr8_2:64001123-64001142 | Msa1261680:CDS | 55.0% | |
| GCACTCAAAAGAGCCTGCAG+GGG | - | chr8_2:64001124-64001143 | Msa1261680:CDS | 55.0% | |
| CACTCAAAAGAGCCTGCAGG+GGG | - | chr8_2:64001125-64001144 | Msa1261680:CDS | 55.0% | |
| AACAATCTTCTGCCCCCTGC+AGG | + | chr8_2:64001140-64001159 | None:intergenic | 55.0% | |
| GCCCCTTGAAACTCCCAAAG+AGG | + | chr8_2:64001262-64001281 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_2 | gene | 64001096 | 64001438 | 64001096 | ID=Msa1261680;Name=Msa1261680 |
| chr8_2 | mRNA | 64001096 | 64001438 | 64001096 | ID=Msa1261680-mRNA-1;Parent=Msa1261680;Name=Msa1261680-mRNA-1;_AED=0.44;_eAED=0.55;_QI=0|0|0|1|1|1|2|0|84 |
| chr8_2 | exon | 64001234 | 64001438 | 64001234 | ID=Msa1261680-mRNA-1:exon:20759;Parent=Msa1261680-mRNA-1 |
| chr8_2 | exon | 64001096 | 64001145 | 64001096 | ID=Msa1261680-mRNA-1:exon:20758;Parent=Msa1261680-mRNA-1 |
| chr8_2 | CDS | 64001234 | 64001438 | 64001234 | ID=Msa1261680-mRNA-1:cds;Parent=Msa1261680-mRNA-1 |
| chr8_2 | CDS | 64001096 | 64001145 | 64001096 | ID=Msa1261680-mRNA-1:cds;Parent=Msa1261680-mRNA-1 |
| Gene Sequence |
| Protein sequence |
