Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1431560 | KEH32101.1 | 95.652 | 69 | 3 | 0 | 1 | 69 | 1 | 69 | 7.14e-40 | 138 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1431560 | A0A072URW0 | 95.652 | 69 | 3 | 0 | 1 | 69 | 1 | 69 | 3.41e-40 | 138 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0001290 | Msa1431560 | 0.977235 | 2.232630e-143 | -8.615850e-47 |
| Msa0052300 | Msa1431560 | 0.826953 | 2.043905e-54 | -8.615850e-47 |
| Msa0052350 | Msa1431560 | 0.821791 | 3.346725e-53 | -8.615850e-47 |
| Msa0055180 | Msa1431560 | -0.816085 | 6.631211e-52 | -8.615850e-47 |
| Msa0097360 | Msa1431560 | 0.813323 | 2.712517e-51 | -8.615850e-47 |
| Msa1368120 | Msa1431560 | -0.811982 | 5.331123e-51 | -8.615850e-47 |
| Msa1418960 | Msa1431560 | 0.819713 | 1.005391e-52 | -8.615850e-47 |
| Msa1431560 | Msa1435170 | 0.811647 | 6.305857e-51 | -8.615850e-47 |
| Msa0603570 | Msa1431560 | -0.836505 | 8.998060e-57 | -8.615850e-47 |
| Msa0615580 | Msa1431560 | -0.819600 | 1.066418e-52 | -8.615850e-47 |
| Msa0620890 | Msa1431560 | -0.808555 | 2.924030e-50 | -8.615850e-47 |
| Msa0661300 | Msa1431560 | -0.818725 | 1.687542e-52 | -8.615850e-47 |
| Msa0818770 | Msa1431560 | -0.804133 | 2.500330e-49 | -8.615850e-47 |
| Msa0146070 | Msa1431560 | 0.816187 | 6.292154e-52 | -8.615850e-47 |
| Msa0162130 | Msa1431560 | -0.804227 | 2.389900e-49 | -8.615850e-47 |
| Msa0190120 | Msa1431560 | -0.804666 | 1.936314e-49 | -8.615850e-47 |
| Msa0207670 | Msa1431560 | -0.810152 | 1.328611e-50 | -8.615850e-47 |
| Msa0209350 | Msa1431560 | -0.806850 | 6.733822e-50 | -8.615850e-47 |
| Msa0209500 | Msa1431560 | -0.801000 | 1.107026e-48 | -8.615850e-47 |
| Msa1261680 | Msa1431560 | 0.993005 | 7.844756e-197 | -8.615850e-47 |
| Msa1296830 | Msa1431560 | -0.831407 | 1.699249e-55 | -8.615850e-47 |
| Msa1343290 | Msa1431560 | -0.824447 | 8.033085e-54 | -8.615850e-47 |
| Msa0370160 | Msa1431560 | 0.801652 | 8.139214e-49 | -8.615850e-47 |
| Msa0414800 | Msa1431560 | -0.804882 | 1.744755e-49 | -8.615850e-47 |
| Msa0417610 | Msa1431560 | 0.820657 | 6.108835e-53 | -8.615850e-47 |
| Msa0437690 | Msa1431560 | 0.828436 | 9.002484e-55 | -8.615850e-47 |
| Msa0443390 | Msa1431560 | -0.821613 | 3.678624e-53 | -8.615850e-47 |
| Msa0458060 | Msa1431560 | -0.801217 | 9.990843e-49 | -8.615850e-47 |
| Msa0981640 | Msa1431560 | 0.987467 | 2.304037e-170 | -8.615850e-47 |
| Msa1019260 | Msa1431560 | -0.805222 | 1.481157e-49 | -8.615850e-47 |
| Msa1104210 | Msa1431560 | -0.817415 | 3.336688e-52 | -8.615850e-47 |
| Msa0265430 | Msa1431560 | -0.816864 | 4.438841e-52 | -8.615850e-47 |
| Msa0271890 | Msa1431560 | -0.802794 | 4.736883e-49 | -8.615850e-47 |
| Msa0340380 | Msa1431560 | -0.811118 | 8.213677e-51 | -8.615850e-47 |
| Msa1126830 | Msa1431560 | -0.815133 | 1.080566e-51 | -8.615850e-47 |
| Msa1165780 | Msa1431560 | -0.837403 | 5.306753e-57 | -8.615850e-47 |
| Msa1166870 | Msa1431560 | -0.814227 | 1.714940e-51 | -8.615850e-47 |
| Msa0509950 | Msa1431560 | 0.833609 | 4.832862e-56 | -8.615850e-47 |
| Msa0534900 | Msa1431560 | -0.800506 | 1.396020e-48 | -8.615850e-47 |
| Msa0563260 | Msa1431560 | 0.810357 | 1.199841e-50 | -8.615850e-47 |
| Msa0581110 | Msa1431560 | -0.811138 | 8.132507e-51 | -8.615850e-47 |
| Msa0586520 | Msa1431560 | 0.819512 | 1.116977e-52 | -8.615850e-47 |
| Msa0588260 | Msa1431560 | -0.816928 | 4.294466e-52 | -8.615850e-47 |
| Msa0881270 | Msa1431560 | -0.820992 | 5.115768e-53 | -8.615850e-47 |
| Msa0974950 | Msa1431560 | -0.802219 | 6.224967e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1431560 | MtrunA17_Chr4g0063041 | 95.652 | 69 | 3 | 0 | 1 | 69 | 1 | 69 | 6.57e-44 | 138 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 28 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAACTTCAATGGGTGCTTC+AGG | 0.232199 | tig0026843:-18863 | Msa1431560:CDS |
| ATCTCCTTTGGAAATTTCAT+TGG | 0.312886 | tig0026843:-18885 | Msa1431560:CDS |
| AAATCCTGTTAAGACCTCTT+TGG | 0.339860 | tig0026843:-18837 | Msa1431560:CDS |
| AATTTCATTGGGAACTTCAA+TGG | 0.362255 | tig0026843:-18873 | Msa1431560:CDS |
| GAAACAGCATCTATCTCCTT+TGG | 0.364301 | tig0026843:-18897 | Msa1431560:CDS |
| TGATTCCAACTGTAAACCTT+TGG | 0.370821 | tig0026843:+18691 | None:intergenic |
| AATCCTGTTAAGACCTCTTT+GGG | 0.394298 | tig0026843:-18836 | Msa1431560:CDS |
| ACTCCCAAAGAGGTCTTAAC+AGG | 0.395727 | tig0026843:+18833 | None:intergenic |
| TCTCCTTTGGAAATTTCATT+GGG | 0.430691 | tig0026843:-18884 | Msa1431560:CDS |
| AGTATCATTGCGACCAATGA+TGG | 0.448934 | tig0026843:-18980 | Msa1431560:CDS |
| AGACCTCTTTGGGAGTTTCA+AGG | 0.451544 | tig0026843:-18826 | Msa1431560:CDS |
| ATTTCATTGGGAACTTCAAT+GGG | 0.452362 | tig0026843:-18872 | Msa1431560:CDS |
| GAACTTCAATGGGTGCTTCA+GGG | 0.458319 | tig0026843:-18862 | Msa1431560:CDS |
| AGCTTCTAACTAACTATCAT+AGG | 0.471561 | tig0026843:+18657 | None:intergenic |
| GGCACTCAAAAGAGCCTGCA+GGG | 0.483137 | tig0026843:-18959 | Msa1431560:CDS |
| CCAATAACCATGAAAGAAAA+CGG | 0.484257 | tig0026843:+18919 | None:intergenic |
| ATGAGTACATGTAGCTCTAA+AGG | 0.492159 | tig0026843:+18796 | None:intergenic |
| GACCTCTTTGGGAGTTTCAA+GGG | 0.493603 | tig0026843:-18825 | Msa1431560:CDS |
| CAGGTCCAAAGGTTTACAGT+TGG | 0.521736 | tig0026843:-18696 | Msa1431560:CDS |
| AACTTCAATGGGTGCTTCAG+GGG | 0.554992 | tig0026843:-18861 | Msa1431560:CDS |
| TGGCACTCAAAAGAGCCTGC+AGG | 0.555623 | tig0026843:-18960 | Msa1431560:CDS |
| GTGGAACTTTGCAGGTCCAA+AGG | 0.556653 | tig0026843:-18707 | Msa1431560:intron |
| ACCTCTTTGGGAGTTTCAAG+GGG | 0.579127 | tig0026843:-18824 | Msa1431560:CDS |
| GTTCCCAATGAAATTTCCAA+AGG | 0.582396 | tig0026843:+18881 | None:intergenic |
| GCCCCTTGAAACTCCCAAAG+AGG | 0.583572 | tig0026843:+18823 | None:intergenic |
| ACTTCAATGGGTGCTTCAGG+GGG | 0.595903 | tig0026843:-18860 | Msa1431560:CDS |
| CACTCAAAAGAGCCTGCAGG+GGG | 0.609954 | tig0026843:-18957 | Msa1431560:CDS |
| GCACTCAAAAGAGCCTGCAG+GGG | 0.624648 | tig0026843:-18958 | Msa1431560:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTATGAACAAATCTTGTGTA+AGG | - | tig0026843:18916-18935 | Msa1431560:CDS | 25.0% |
| CCAATAACCATGAAAGAAAA+CGG | + | tig0026843:18736-18755 | None:intergenic | 30.0% | |
| ! | ATCTCCTTTGGAAATTTCAT+TGG | - | tig0026843:18767-18786 | Msa1431560:intron | 30.0% |
| ! | TCTCCTTTGGAAATTTCATT+GGG | - | tig0026843:18768-18787 | Msa1431560:intron | 30.0% |
| AATTTCATTGGGAACTTCAA+TGG | - | tig0026843:18779-18798 | Msa1431560:intron | 30.0% | |
| ATTTCATTGGGAACTTCAAT+GGG | - | tig0026843:18780-18799 | Msa1431560:intron | 30.0% | |
| ! | AAGATTTGTTCATAACCTGA+GGG | + | tig0026843:18911-18930 | None:intergenic | 30.0% |
| !! | TGTTTAGCCGTTTTCTTTCA+TGG | - | tig0026843:18726-18745 | Msa1431560:intron | 35.0% |
| ! | CCGTTTTCTTTCATGGTTAT+TGG | - | tig0026843:18733-18752 | Msa1431560:intron | 35.0% |
| GTTCCCAATGAAATTTCCAA+AGG | + | tig0026843:18774-18793 | None:intergenic | 35.0% | |
| AAATCCTGTTAAGACCTCTT+TGG | - | tig0026843:18815-18834 | Msa1431560:CDS | 35.0% | |
| AATCCTGTTAAGACCTCTTT+GGG | - | tig0026843:18816-18835 | Msa1431560:CDS | 35.0% | |
| !! | ATGAGTACATGTAGCTCTAA+AGG | + | tig0026843:18859-18878 | None:intergenic | 35.0% |
| TAGAGCTACATGTACTCATT+CGG | - | tig0026843:18860-18879 | Msa1431560:CDS | 35.0% | |
| TTCATAACCTGAGGGTTTAA+GGG | + | tig0026843:18903-18922 | None:intergenic | 35.0% | |
| ! | CAAGATTTGTTCATAACCTG+AGG | + | tig0026843:18912-18931 | None:intergenic | 35.0% |
| !! | ATCTTGTGTAAGGTTTTGTG+TGG | - | tig0026843:18926-18945 | Msa1431560:CDS | 35.0% |
| TGATTCCAACTGTAAACCTT+TGG | + | tig0026843:18964-18983 | None:intergenic | 35.0% | |
| AGTATCATTGCGACCAATGA+TGG | - | tig0026843:18672-18691 | Msa1431560:CDS | 40.0% | |
| GAAACAGCATCTATCTCCTT+TGG | - | tig0026843:18755-18774 | Msa1431560:intron | 40.0% | |
| GTTCATAACCTGAGGGTTTA+AGG | + | tig0026843:18904-18923 | None:intergenic | 40.0% | |
| !! | GCTCTTTTGAGTGCCATCAT+TGG | + | tig0026843:18688-18707 | None:intergenic | 45.0% |
| GAACTTCAATGGGTGCTTCA+GGG | - | tig0026843:18790-18809 | Msa1431560:intron | 45.0% | |
| AACTTCAATGGGTGCTTCAG+GGG | - | tig0026843:18791-18810 | Msa1431560:intron | 45.0% | |
| ACTCCCAAAGAGGTCTTAAC+AGG | + | tig0026843:18822-18841 | None:intergenic | 45.0% | |
| ! | AGACCTCTTTGGGAGTTTCA+AGG | - | tig0026843:18826-18845 | Msa1431560:CDS | 45.0% |
| ! | GACCTCTTTGGGAGTTTCAA+GGG | - | tig0026843:18827-18846 | Msa1431560:CDS | 45.0% |
| ! | ACCTCTTTGGGAGTTTCAAG+GGG | - | tig0026843:18828-18847 | Msa1431560:CDS | 45.0% |
| !! | GGTTTTGTGTGGAACTTTGC+AGG | - | tig0026843:18937-18956 | Msa1431560:CDS | 45.0% |
| CAGGTCCAAAGGTTTACAGT+TGG | - | tig0026843:18956-18975 | Msa1431560:CDS | 45.0% | |
| GGAACTTCAATGGGTGCTTC+AGG | - | tig0026843:18789-18808 | Msa1431560:intron | 50.0% | |
| ACTTCAATGGGTGCTTCAGG+GGG | - | tig0026843:18792-18811 | Msa1431560:intron | 50.0% | |
| AGTGCATCCCTTAAACCCTC+AGG | - | tig0026843:18893-18912 | Msa1431560:CDS | 50.0% | |
| GTGGAACTTTGCAGGTCCAA+AGG | - | tig0026843:18945-18964 | Msa1431560:CDS | 50.0% | |
| TGGCACTCAAAAGAGCCTGC+AGG | - | tig0026843:18692-18711 | Msa1431560:CDS | 55.0% | |
| GGCACTCAAAAGAGCCTGCA+GGG | - | tig0026843:18693-18712 | Msa1431560:CDS | 55.0% | |
| GCACTCAAAAGAGCCTGCAG+GGG | - | tig0026843:18694-18713 | Msa1431560:CDS | 55.0% | |
| CACTCAAAAGAGCCTGCAGG+GGG | - | tig0026843:18695-18714 | Msa1431560:CDS | 55.0% | |
| AACAATCTTCTGCCCCCTGC+AGG | + | tig0026843:18710-18729 | None:intergenic | 55.0% | |
| GCCCCTTGAAACTCCCAAAG+AGG | + | tig0026843:18832-18851 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0026843 | gene | 18666 | 19008 | 18666 | ID=Msa1431560;Name=Msa1431560 |
| tig0026843 | mRNA | 18666 | 19008 | 18666 | ID=Msa1431560-mRNA-1;Parent=Msa1431560;Name=Msa1431560-mRNA-1;_AED=0.44;_eAED=0.55;_QI=0|0|0|1|1|1|2|0|84 |
| tig0026843 | exon | 18804 | 19008 | 18804 | ID=Msa1431560-mRNA-1:exon:7671;Parent=Msa1431560-mRNA-1 |
| tig0026843 | exon | 18666 | 18715 | 18666 | ID=Msa1431560-mRNA-1:exon:7670;Parent=Msa1431560-mRNA-1 |
| tig0026843 | CDS | 18804 | 19008 | 18804 | ID=Msa1431560-mRNA-1:cds;Parent=Msa1431560-mRNA-1 |
| tig0026843 | CDS | 18666 | 18715 | 18666 | ID=Msa1431560-mRNA-1:cds;Parent=Msa1431560-mRNA-1 |
| Gene Sequence |
| Protein sequence |
