Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0981640 | KEH32101.1 | 77.193 | 114 | 11 | 2 | 1 | 99 | 1 | 114 | 6.37e-51 | 167 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0981640 | A0A072URW0 | 77.193 | 114 | 11 | 2 | 1 | 99 | 1 | 114 | 3.04e-51 | 167 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0001290 | Msa0981640 | 0.983148 | 5.848758e-157 | -8.615850e-47 |
| Msa0052300 | Msa0981640 | 0.810694 | 1.015042e-50 | -8.615850e-47 |
| Msa0052350 | Msa0981640 | 0.800148 | 1.650940e-48 | -8.615850e-47 |
| Msa0603570 | Msa0981640 | -0.806481 | 8.057167e-50 | -8.615850e-47 |
| Msa0146070 | Msa0981640 | 0.805113 | 1.561483e-49 | -8.615850e-47 |
| Msa0981640 | Msa1165780 | -0.807219 | 5.625649e-50 | -8.615850e-47 |
| Msa0981640 | Msa1261680 | 0.985961 | 3.192682e-165 | -8.615850e-47 |
| Msa0981640 | Msa1296830 | -0.800724 | 1.260322e-48 | -8.615850e-47 |
| Msa0981640 | Msa1431560 | 0.987467 | 2.304037e-170 | -8.615850e-47 |
| Msa0265430 | Msa0981640 | -0.808368 | 3.206065e-50 | -8.615850e-47 |
| Msa0340380 | Msa0981640 | -0.803669 | 3.122588e-49 | -8.615850e-47 |
| Msa0509950 | Msa0981640 | 0.804125 | 2.509398e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0981640 | MtrunA17_Chr4g0063041 | 77.193 | 114 | 11 | 2 | 1 | 99 | 1 | 114 | 5.86e-55 | 167 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 32 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTCATAACCTGAGGGTTTA+AGG | 0.187585 | 6_4:-21150704 | None:intergenic |
| GGAACTTCAATGGGTGCTTC+AGG | 0.232199 | 6_4:+21150592 | Msa0981640:CDS |
| ATCTCCTTTGGAAATTTCAT+TGG | 0.312886 | 6_4:+21150570 | Msa0981640:CDS |
| AAATCCTGTTAAGACCTCTT+TGG | 0.339860 | 6_4:+21150618 | Msa0981640:CDS |
| AATTTCATTGGGAACTTCAA+TGG | 0.362255 | 6_4:+21150582 | Msa0981640:CDS |
| GAAACAGCATCTATCTCCTT+TGG | 0.364301 | 6_4:+21150558 | Msa0981640:CDS |
| TGATTCCAACTGTAAACCTT+TGG | 0.370821 | 6_4:-21150764 | None:intergenic |
| TTCATAACCTGAGGGTTTAA+GGG | 0.376944 | 6_4:-21150703 | None:intergenic |
| AATCCTGTTAAGACCTCTTT+GGG | 0.394298 | 6_4:+21150619 | Msa0981640:CDS |
| ACTCCCAAAGAGGTCTTAAC+AGG | 0.395727 | 6_4:-21150622 | None:intergenic |
| TCTCCTTTGGAAATTTCATT+GGG | 0.430691 | 6_4:+21150571 | Msa0981640:CDS |
| AGTATCATTGCGACCAATGA+TGG | 0.448934 | 6_4:+21150475 | Msa0981640:CDS |
| AGACCTCTTTGGGAGTTTCA+AGG | 0.451544 | 6_4:+21150629 | Msa0981640:CDS |
| ATTTCATTGGGAACTTCAAT+GGG | 0.452362 | 6_4:+21150583 | Msa0981640:CDS |
| TAGAGCTACATGTACTCATT+CGG | 0.458255 | 6_4:+21150663 | Msa0981640:CDS |
| GAACTTCAATGGGTGCTTCA+GGG | 0.458319 | 6_4:+21150593 | Msa0981640:CDS |
| AGCTTCTAACTAACTATCAT+AGG | 0.471561 | 6_4:-21150798 | None:intergenic |
| GGCACTCAAAAGAGCCTGCA+GGG | 0.483137 | 6_4:+21150496 | Msa0981640:CDS |
| CCAATAACCATGAAAGAAAA+CGG | 0.484257 | 6_4:-21150536 | None:intergenic |
| ATGAGTACATGTAGCTCTAA+AGG | 0.492159 | 6_4:-21150659 | None:intergenic |
| GACCTCTTTGGGAGTTTCAA+GGG | 0.493603 | 6_4:+21150630 | Msa0981640:CDS |
| CAGGTCCAAAGGTTTACAGT+TGG | 0.521736 | 6_4:+21150759 | Msa0981640:intron |
| AACTTCAATGGGTGCTTCAG+GGG | 0.554992 | 6_4:+21150594 | Msa0981640:CDS |
| TGGCACTCAAAAGAGCCTGC+AGG | 0.555623 | 6_4:+21150495 | Msa0981640:CDS |
| GTGGAACTTTGCAGGTCCAA+AGG | 0.556653 | 6_4:+21150748 | Msa0981640:intron |
| AGTGCATCCCTTAAACCCTC+AGG | 0.561133 | 6_4:+21150696 | Msa0981640:CDS |
| ACCTCTTTGGGAGTTTCAAG+GGG | 0.579127 | 6_4:+21150631 | Msa0981640:CDS |
| GTTCCCAATGAAATTTCCAA+AGG | 0.582396 | 6_4:-21150574 | None:intergenic |
| GCCCCTTGAAACTCCCAAAG+AGG | 0.583572 | 6_4:-21150632 | None:intergenic |
| ACTTCAATGGGTGCTTCAGG+GGG | 0.595903 | 6_4:+21150595 | Msa0981640:CDS |
| CACTCAAAAGAGCCTGCAGG+GGG | 0.609954 | 6_4:+21150498 | Msa0981640:CDS |
| GCACTCAAAAGAGCCTGCAG+GGG | 0.624648 | 6_4:+21150497 | Msa0981640:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTATGAACAAATCTTGTGTA+AGG | + | chr6_4:21150719-21150738 | Msa0981640:intron | 25.0% |
| CCAATAACCATGAAAGAAAA+CGG | - | chr6_4:21150539-21150558 | None:intergenic | 30.0% | |
| ! | ATCTCCTTTGGAAATTTCAT+TGG | + | chr6_4:21150570-21150589 | Msa0981640:CDS | 30.0% |
| ! | TCTCCTTTGGAAATTTCATT+GGG | + | chr6_4:21150571-21150590 | Msa0981640:CDS | 30.0% |
| AATTTCATTGGGAACTTCAA+TGG | + | chr6_4:21150582-21150601 | Msa0981640:CDS | 30.0% | |
| ATTTCATTGGGAACTTCAAT+GGG | + | chr6_4:21150583-21150602 | Msa0981640:CDS | 30.0% | |
| ! | AAGATTTGTTCATAACCTGA+GGG | - | chr6_4:21150714-21150733 | None:intergenic | 30.0% |
| !! | TGTTTAGCCGTTTTCTTTCA+TGG | + | chr6_4:21150529-21150548 | Msa0981640:CDS | 35.0% |
| ! | CCGTTTTCTTTCATGGTTAT+TGG | + | chr6_4:21150536-21150555 | Msa0981640:CDS | 35.0% |
| GTTCCCAATGAAATTTCCAA+AGG | - | chr6_4:21150577-21150596 | None:intergenic | 35.0% | |
| AAATCCTGTTAAGACCTCTT+TGG | + | chr6_4:21150618-21150637 | Msa0981640:CDS | 35.0% | |
| AATCCTGTTAAGACCTCTTT+GGG | + | chr6_4:21150619-21150638 | Msa0981640:CDS | 35.0% | |
| !! | ATGAGTACATGTAGCTCTAA+AGG | - | chr6_4:21150662-21150681 | None:intergenic | 35.0% |
| TAGAGCTACATGTACTCATT+CGG | + | chr6_4:21150663-21150682 | Msa0981640:CDS | 35.0% | |
| TTCATAACCTGAGGGTTTAA+GGG | - | chr6_4:21150706-21150725 | None:intergenic | 35.0% | |
| ! | CAAGATTTGTTCATAACCTG+AGG | - | chr6_4:21150715-21150734 | None:intergenic | 35.0% |
| !! | ATCTTGTGTAAGGTTTTGTG+TGG | + | chr6_4:21150729-21150748 | Msa0981640:intron | 35.0% |
| TGATTCCAACTGTAAACCTT+TGG | - | chr6_4:21150767-21150786 | None:intergenic | 35.0% | |
| AGTATCATTGCGACCAATGA+TGG | + | chr6_4:21150475-21150494 | Msa0981640:CDS | 40.0% | |
| GAAACAGCATCTATCTCCTT+TGG | + | chr6_4:21150558-21150577 | Msa0981640:CDS | 40.0% | |
| GTTCATAACCTGAGGGTTTA+AGG | - | chr6_4:21150707-21150726 | None:intergenic | 40.0% | |
| !! | GCTCTTTTGAGTGCCATCAT+TGG | - | chr6_4:21150491-21150510 | None:intergenic | 45.0% |
| GAACTTCAATGGGTGCTTCA+GGG | + | chr6_4:21150593-21150612 | Msa0981640:CDS | 45.0% | |
| AACTTCAATGGGTGCTTCAG+GGG | + | chr6_4:21150594-21150613 | Msa0981640:CDS | 45.0% | |
| ACTCCCAAAGAGGTCTTAAC+AGG | - | chr6_4:21150625-21150644 | None:intergenic | 45.0% | |
| ! | AGACCTCTTTGGGAGTTTCA+AGG | + | chr6_4:21150629-21150648 | Msa0981640:CDS | 45.0% |
| ! | GACCTCTTTGGGAGTTTCAA+GGG | + | chr6_4:21150630-21150649 | Msa0981640:CDS | 45.0% |
| ! | ACCTCTTTGGGAGTTTCAAG+GGG | + | chr6_4:21150631-21150650 | Msa0981640:CDS | 45.0% |
| !! | GGTTTTGTGTGGAACTTTGC+AGG | + | chr6_4:21150740-21150759 | Msa0981640:intron | 45.0% |
| CAGGTCCAAAGGTTTACAGT+TGG | + | chr6_4:21150759-21150778 | Msa0981640:intron | 45.0% | |
| GGAACTTCAATGGGTGCTTC+AGG | + | chr6_4:21150592-21150611 | Msa0981640:CDS | 50.0% | |
| ACTTCAATGGGTGCTTCAGG+GGG | + | chr6_4:21150595-21150614 | Msa0981640:CDS | 50.0% | |
| AGTGCATCCCTTAAACCCTC+AGG | + | chr6_4:21150696-21150715 | Msa0981640:CDS | 50.0% | |
| GTGGAACTTTGCAGGTCCAA+AGG | + | chr6_4:21150748-21150767 | Msa0981640:intron | 50.0% | |
| TGGCACTCAAAAGAGCCTGC+AGG | + | chr6_4:21150495-21150514 | Msa0981640:CDS | 55.0% | |
| GGCACTCAAAAGAGCCTGCA+GGG | + | chr6_4:21150496-21150515 | Msa0981640:CDS | 55.0% | |
| GCACTCAAAAGAGCCTGCAG+GGG | + | chr6_4:21150497-21150516 | Msa0981640:CDS | 55.0% | |
| CACTCAAAAGAGCCTGCAGG+GGG | + | chr6_4:21150498-21150517 | Msa0981640:CDS | 55.0% | |
| AACAATCTTCTGCCCCCTGC+AGG | - | chr6_4:21150513-21150532 | None:intergenic | 55.0% | |
| GCCCCTTGAAACTCCCAAAG+AGG | - | chr6_4:21150635-21150654 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_4 | gene | 21150469 | 21150815 | 21150469 | ID=Msa0981640;Name=Msa0981640 |
| chr6_4 | mRNA | 21150469 | 21150815 | 21150469 | ID=Msa0981640-mRNA-1;Parent=Msa0981640;Name=Msa0981640-mRNA-1;_AED=0.43;_eAED=0.53;_QI=0|0|0|1|1|1|2|0|100 |
| chr6_4 | exon | 21150469 | 21150717 | 21150469 | ID=Msa0981640-mRNA-1:exon:6480;Parent=Msa0981640-mRNA-1 |
| chr6_4 | exon | 21150762 | 21150815 | 21150762 | ID=Msa0981640-mRNA-1:exon:6481;Parent=Msa0981640-mRNA-1 |
| chr6_4 | CDS | 21150469 | 21150717 | 21150469 | ID=Msa0981640-mRNA-1:cds;Parent=Msa0981640-mRNA-1 |
| chr6_4 | CDS | 21150762 | 21150815 | 21150762 | ID=Msa0981640-mRNA-1:cds;Parent=Msa0981640-mRNA-1 |
| Gene Sequence |
| Protein sequence |
