Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0011790 | Msa0021340 | 0.858622 | 7.257588e-63 | -8.615850e-47 |
| Msa0021340 | Msa0229630 | 0.825171 | 5.420581e-54 | -8.615850e-47 |
| Msa0021340 | Msa0968410 | 0.801682 | 8.023674e-49 | -8.615850e-47 |
| Msa0021340 | Msa0968430 | 0.821591 | 3.722320e-53 | -8.615850e-47 |
| Msa0021340 | Msa1389180 | 0.809075 | 2.263577e-50 | -8.615850e-47 |
| Msa0021340 | Msa1389200 | 0.862082 | 6.518049e-64 | -8.615850e-47 |
| Msa0021340 | Msa1422510 | 0.801015 | 1.098990e-48 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0021340 | MtrunA17_Chr1g0160761 | 83.636 | 55 | 8 | 1 | 1 | 55 | 1 | 54 | 6.50e-25 | 98.6 |
| Msa0021340 | MtrunA17_Chr1g0160721 | 81.481 | 54 | 9 | 1 | 1 | 54 | 12 | 64 | 5.32e-22 | 90.5 |
| Msa0021340 | MtrunA17_Chr1g0160661 | 65.455 | 55 | 18 | 1 | 1 | 55 | 27 | 80 | 4.00e-17 | 76.3 |
| Msa0021340 | MtrunA17_Chr1g0160561 | 61.111 | 54 | 19 | 2 | 2 | 55 | 7 | 58 | 1.90e-16 | 74.7 |
| Msa0021340 | MtrunA17_Chr1g0160701 | 79.487 | 39 | 7 | 1 | 17 | 55 | 32 | 69 | 3.93e-15 | 70.9 |
| Msa0021340 | MtrunA17_Chr1g0160711 | 77.778 | 36 | 6 | 2 | 21 | 55 | 17 | 51 | 1.09e-11 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 23 sgRNAs with CRISPR-Local
Find 102 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTATGGATTTGAACTTTCT+TGG | 0.219600 | 1_1:-38008899 | Msa0021340:CDS |
| TCTCTTTCAGATTTGGGACT+TGG | 0.355277 | 1_1:-38009014 | Msa0021340:CDS |
| CTCAATAGTCTTTGTAATGA+AGG | 0.356928 | 1_1:-38008876 | Msa0021340:CDS |
| CAGATTTGGGACTTGGAGAC+GGG | 0.442912 | 1_1:-38009007 | Msa0021340:CDS |
| ACTTGGAGACGGGTGTCATA+TGG | 0.443107 | 1_1:-38008997 | Msa0021340:CDS |
| TAACAGCACGACAAGGGTTA+AGG | 0.463192 | 1_1:+38009550 | None:intergenic |
| ACATCATGGCCTCTTGAAGA+TGG | 0.468797 | 1_1:-38008960 | Msa0021340:CDS |
| GAGAATTCTTTCTATGTTGG+TGG | 0.469005 | 1_1:-38009047 | Msa0021340:CDS |
| CTCTTGTCACCACATTCGTC+TGG | 0.472075 | 1_1:+38009413 | None:intergenic |
| TACGAGAATTCTTTCTATGT+TGG | 0.482804 | 1_1:-38009050 | Msa0021340:CDS |
| TCAGATTTGGGACTTGGAGA+CGG | 0.489585 | 1_1:-38009008 | Msa0021340:CDS |
| CTGGATCTATACATAAATGC+TGG | 0.539585 | 1_1:+38008826 | None:intergenic |
| TTCTACAGACATGCATCGAA+TGG | 0.562503 | 1_1:-38008925 | Msa0021340:CDS |
| AATCTATGTATCTCACATCA+TGG | 0.571651 | 1_1:-38008974 | Msa0021340:CDS |
| TTCTCGTACAACAAAGTGCA+TGG | 0.590113 | 1_1:-38009515 | Msa0021340:CDS |
| AACAAGAGTCATGTTCAAGA+AGG | 0.596613 | 1_1:-38009484 | Msa0021340:CDS |
| ATATTGTTTCCATCTTCAAG+AGG | 0.599725 | 1_1:+38008951 | None:intergenic |
| AAGAGTCATGTTCAAGAAGG+TGG | 0.600098 | 1_1:-38009481 | Msa0021340:CDS |
| GTATTAGTAACAGCACGACA+AGG | 0.628217 | 1_1:+38009543 | None:intergenic |
| CAGACGAATGTGGTGACAAG+AGG | 0.643613 | 1_1:-38009412 | Msa0021340:intron |
| TATTAGTAACAGCACGACAA+GGG | 0.651789 | 1_1:+38009544 | None:intergenic |
| TCATGTTCAAGAAGGTGGTG+CGG | 0.678993 | 1_1:-38009476 | Msa0021340:CDS |
| AAAGACAGTCCAGACGAATG+TGG | 0.680868 | 1_1:-38009422 | Msa0021340:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTGTACATAATATTTTCCA+TGG | + | chr1_1:38009067-38009086 | None:intergenic | 20.0% |
| !!! | ATTGTACATAATATTTTCCA+TGG | + | chr1_1:38009067-38009086 | None:intergenic | 20.0% |
| ! | TATATGTGAGCTGTAAAAAT+CGG | - | chr1_1:38009267-38009286 | Msa0021340:intron | 25.0% |
| ! | CAAAAATCTCTTTCAGATTT+GGG | - | chr1_1:38009374-38009393 | Msa0021340:intron | 25.0% |
| ! | TTTATGGATTTGAACTTTCT+TGG | - | chr1_1:38009495-38009514 | Msa0021340:CDS | 25.0% |
| ! | TATATGTGAGCTGTAAAAAT+CGG | - | chr1_1:38009267-38009286 | Msa0021340:intron | 25.0% |
| ! | CAAAAATCTCTTTCAGATTT+GGG | - | chr1_1:38009374-38009393 | Msa0021340:intron | 25.0% |
| ! | TTTATGGATTTGAACTTTCT+TGG | - | chr1_1:38009495-38009514 | Msa0021340:CDS | 25.0% |
| CATTATTGTCCTCACATAAT+AGG | + | chr1_1:38009013-38009032 | None:intergenic | 30.0% | |
| TATGAATTTGAAGAACACCA+TGG | - | chr1_1:38009047-38009066 | Msa0021340:CDS | 30.0% | |
| ATTGAAATCCAATAGTCGTA+TGG | + | chr1_1:38009111-38009130 | None:intergenic | 30.0% | |
| TAAAGAATAATAAGGAGGGA+TGG | + | chr1_1:38009150-38009169 | None:intergenic | 30.0% | |
| ATCCCTCCTTATTATTCTTT+AGG | - | chr1_1:38009149-38009168 | Msa0021340:intron | 30.0% | |
| TCCCTCCTTATTATTCTTTA+GGG | - | chr1_1:38009150-38009169 | Msa0021340:intron | 30.0% | |
| TAGAGCCCTAAAGAATAATA+AGG | + | chr1_1:38009158-38009177 | None:intergenic | 30.0% | |
| TATCCATTAAAAGCTAGGTA+GGG | + | chr1_1:38009209-38009228 | None:intergenic | 30.0% | |
| TTTCGTATCCATTAAAAGCT+AGG | + | chr1_1:38009214-38009233 | None:intergenic | 30.0% | |
| TACGAGAATTCTTTCTATGT+TGG | - | chr1_1:38009344-38009363 | Msa0021340:intron | 30.0% | |
| ! | CCAAATCTGAAAGAGATTTT+TGG | + | chr1_1:38009376-38009395 | None:intergenic | 30.0% |
| CCAAAAATCTCTTTCAGATT+TGG | - | chr1_1:38009373-38009392 | Msa0021340:intron | 30.0% | |
| AATCTATGTATCTCACATCA+TGG | - | chr1_1:38009420-38009439 | Msa0021340:CDS | 30.0% | |
| ATATTGTTTCCATCTTCAAG+AGG | + | chr1_1:38009446-38009465 | None:intergenic | 30.0% | |
| CTCAATAGTCTTTGTAATGA+AGG | - | chr1_1:38009518-38009537 | Msa0021340:CDS | 30.0% | |
| CATTATTGTCCTCACATAAT+AGG | + | chr1_1:38009013-38009032 | None:intergenic | 30.0% | |
| TATGAATTTGAAGAACACCA+TGG | - | chr1_1:38009047-38009066 | Msa0021340:CDS | 30.0% | |
| ATTGAAATCCAATAGTCGTA+TGG | + | chr1_1:38009111-38009130 | None:intergenic | 30.0% | |
| TAAAGAATAATAAGGAGGGA+TGG | + | chr1_1:38009150-38009169 | None:intergenic | 30.0% | |
| ATCCCTCCTTATTATTCTTT+AGG | - | chr1_1:38009149-38009168 | Msa0021340:intron | 30.0% | |
| TCCCTCCTTATTATTCTTTA+GGG | - | chr1_1:38009150-38009169 | Msa0021340:intron | 30.0% | |
| TAGAGCCCTAAAGAATAATA+AGG | + | chr1_1:38009158-38009177 | None:intergenic | 30.0% | |
| TATCCATTAAAAGCTAGGTA+GGG | + | chr1_1:38009209-38009228 | None:intergenic | 30.0% | |
| TTTCGTATCCATTAAAAGCT+AGG | + | chr1_1:38009214-38009233 | None:intergenic | 30.0% | |
| TACGAGAATTCTTTCTATGT+TGG | - | chr1_1:38009344-38009363 | Msa0021340:intron | 30.0% | |
| ! | CCAAATCTGAAAGAGATTTT+TGG | + | chr1_1:38009376-38009395 | None:intergenic | 30.0% |
| CCAAAAATCTCTTTCAGATT+TGG | - | chr1_1:38009373-38009392 | Msa0021340:intron | 30.0% | |
| AATCTATGTATCTCACATCA+TGG | - | chr1_1:38009420-38009439 | Msa0021340:CDS | 30.0% | |
| ATATTGTTTCCATCTTCAAG+AGG | + | chr1_1:38009446-38009465 | None:intergenic | 30.0% | |
| CTCAATAGTCTTTGTAATGA+AGG | - | chr1_1:38009518-38009537 | Msa0021340:CDS | 30.0% | |
| TATTAGTAACAGCACGACAA+GGG | + | chr1_1:38008853-38008872 | None:intergenic | 35.0% | |
| AACAAGAGTCATGTTCAAGA+AGG | - | chr1_1:38008910-38008929 | Msa0021340:CDS | 35.0% | |
| ! | ATAACATTAGCCCTCCTTTT+AGG | - | chr1_1:38008946-38008965 | Msa0021340:CDS | 35.0% |
| ! | TGTCTTTTAGCCTAAAAGGA+GGG | + | chr1_1:38008959-38008978 | None:intergenic | 35.0% |
| GCCCTAAAGAATAATAAGGA+GGG | + | chr1_1:38009154-38009173 | None:intergenic | 35.0% | |
| AGCCCTAAAGAATAATAAGG+AGG | + | chr1_1:38009155-38009174 | None:intergenic | 35.0% | |
| GTATCCATTAAAAGCTAGGT+AGG | + | chr1_1:38009210-38009229 | None:intergenic | 35.0% | |
| TCGGAATATATTGATGAAGG+TGG | - | chr1_1:38009290-38009309 | Msa0021340:intron | 35.0% | |
| GAGAATTCTTTCTATGTTGG+TGG | - | chr1_1:38009347-38009366 | Msa0021340:intron | 35.0% | |
| ! | ATGCATCGAATGGTGTTTTA+TGG | - | chr1_1:38009479-38009498 | Msa0021340:CDS | 35.0% |
| TATTAGTAACAGCACGACAA+GGG | + | chr1_1:38008853-38008872 | None:intergenic | 35.0% | |
| AACAAGAGTCATGTTCAAGA+AGG | - | chr1_1:38008910-38008929 | Msa0021340:CDS | 35.0% | |
| ! | ATAACATTAGCCCTCCTTTT+AGG | - | chr1_1:38008946-38008965 | Msa0021340:CDS | 35.0% |
| ! | TGTCTTTTAGCCTAAAAGGA+GGG | + | chr1_1:38008959-38008978 | None:intergenic | 35.0% |
| GCCCTAAAGAATAATAAGGA+GGG | + | chr1_1:38009154-38009173 | None:intergenic | 35.0% | |
| AGCCCTAAAGAATAATAAGG+AGG | + | chr1_1:38009155-38009174 | None:intergenic | 35.0% | |
| GTATCCATTAAAAGCTAGGT+AGG | + | chr1_1:38009210-38009229 | None:intergenic | 35.0% | |
| TCGGAATATATTGATGAAGG+TGG | - | chr1_1:38009290-38009309 | Msa0021340:intron | 35.0% | |
| GAGAATTCTTTCTATGTTGG+TGG | - | chr1_1:38009347-38009366 | Msa0021340:intron | 35.0% | |
| ! | ATGCATCGAATGGTGTTTTA+TGG | - | chr1_1:38009479-38009498 | Msa0021340:CDS | 35.0% |
| GTATTAGTAACAGCACGACA+AGG | + | chr1_1:38008854-38008873 | None:intergenic | 40.0% | |
| TTCTCGTACAACAAAGTGCA+TGG | - | chr1_1:38008879-38008898 | Msa0021340:CDS | 40.0% | |
| AAGAGTCATGTTCAAGAAGG+TGG | - | chr1_1:38008913-38008932 | Msa0021340:CDS | 40.0% | |
| ! | CTGTCTTTTAGCCTAAAAGG+AGG | + | chr1_1:38008960-38008979 | None:intergenic | 40.0% |
| ! | GGACTGTCTTTTAGCCTAAA+AGG | + | chr1_1:38008963-38008982 | None:intergenic | 40.0% |
| GAGGTACAACCTATTATGTG+AGG | - | chr1_1:38009001-38009020 | Msa0021340:CDS | 40.0% | |
| CAACTTCACCATACGACTAT+TGG | - | chr1_1:38009100-38009119 | Msa0021340:intron | 40.0% | |
| ATTAAAAGCTAGGTAGGGTG+AGG | + | chr1_1:38009204-38009223 | None:intergenic | 40.0% | |
| !! | TCACCCTACCTAGCTTTTAA+TGG | - | chr1_1:38009203-38009222 | Msa0021340:intron | 40.0% |
| TGTGAGCTGTAAAAATCGGT+CGG | - | chr1_1:38009271-38009290 | Msa0021340:intron | 40.0% | |
| CGGTCGGAATATATTGATGA+AGG | - | chr1_1:38009287-38009306 | Msa0021340:intron | 40.0% | |
| ! | TCTCTTTCAGATTTGGGACT+TGG | - | chr1_1:38009380-38009399 | Msa0021340:intron | 40.0% |
| TTCTACAGACATGCATCGAA+TGG | - | chr1_1:38009469-38009488 | Msa0021340:CDS | 40.0% | |
| GTATTAGTAACAGCACGACA+AGG | + | chr1_1:38008854-38008873 | None:intergenic | 40.0% | |
| TTCTCGTACAACAAAGTGCA+TGG | - | chr1_1:38008879-38008898 | Msa0021340:CDS | 40.0% | |
| AAGAGTCATGTTCAAGAAGG+TGG | - | chr1_1:38008913-38008932 | Msa0021340:CDS | 40.0% | |
| ! | CTGTCTTTTAGCCTAAAAGG+AGG | + | chr1_1:38008960-38008979 | None:intergenic | 40.0% |
| ! | GGACTGTCTTTTAGCCTAAA+AGG | + | chr1_1:38008963-38008982 | None:intergenic | 40.0% |
| GAGGTACAACCTATTATGTG+AGG | - | chr1_1:38009001-38009020 | Msa0021340:CDS | 40.0% | |
| CAACTTCACCATACGACTAT+TGG | - | chr1_1:38009100-38009119 | Msa0021340:intron | 40.0% | |
| ATTAAAAGCTAGGTAGGGTG+AGG | + | chr1_1:38009204-38009223 | None:intergenic | 40.0% | |
| !! | TCACCCTACCTAGCTTTTAA+TGG | - | chr1_1:38009203-38009222 | Msa0021340:intron | 40.0% |
| TGTGAGCTGTAAAAATCGGT+CGG | - | chr1_1:38009271-38009290 | Msa0021340:intron | 40.0% | |
| CGGTCGGAATATATTGATGA+AGG | - | chr1_1:38009287-38009306 | Msa0021340:intron | 40.0% | |
| ! | TCTCTTTCAGATTTGGGACT+TGG | - | chr1_1:38009380-38009399 | Msa0021340:intron | 40.0% |
| TTCTACAGACATGCATCGAA+TGG | - | chr1_1:38009469-38009488 | Msa0021340:CDS | 40.0% | |
| TAACAGCACGACAAGGGTTA+AGG | + | chr1_1:38008847-38008866 | None:intergenic | 45.0% | |
| TCATGTTCAAGAAGGTGGTG+CGG | - | chr1_1:38008918-38008937 | Msa0021340:CDS | 45.0% | |
| AAAGACAGTCCAGACGAATG+TGG | - | chr1_1:38008972-38008991 | Msa0021340:CDS | 45.0% | |
| ! | TCAGATTTGGGACTTGGAGA+CGG | - | chr1_1:38009386-38009405 | Msa0021340:intron | 45.0% |
| ACATCATGGCCTCTTGAAGA+TGG | - | chr1_1:38009434-38009453 | Msa0021340:CDS | 45.0% | |
| TAACAGCACGACAAGGGTTA+AGG | + | chr1_1:38008847-38008866 | None:intergenic | 45.0% | |
| TCATGTTCAAGAAGGTGGTG+CGG | - | chr1_1:38008918-38008937 | Msa0021340:CDS | 45.0% | |
| AAAGACAGTCCAGACGAATG+TGG | - | chr1_1:38008972-38008991 | Msa0021340:CDS | 45.0% | |
| ! | TCAGATTTGGGACTTGGAGA+CGG | - | chr1_1:38009386-38009405 | Msa0021340:intron | 45.0% |
| ACATCATGGCCTCTTGAAGA+TGG | - | chr1_1:38009434-38009453 | Msa0021340:CDS | 45.0% | |
| CTCTTGTCACCACATTCGTC+TGG | + | chr1_1:38008984-38009003 | None:intergenic | 50.0% | |
| CAGACGAATGTGGTGACAAG+AGG | - | chr1_1:38008982-38009001 | Msa0021340:CDS | 50.0% | |
| ! | CAGATTTGGGACTTGGAGAC+GGG | - | chr1_1:38009387-38009406 | Msa0021340:intron | 50.0% |
| ! | ACTTGGAGACGGGTGTCATA+TGG | - | chr1_1:38009397-38009416 | Msa0021340:intron | 50.0% |
| CTCTTGTCACCACATTCGTC+TGG | + | chr1_1:38008984-38009003 | None:intergenic | 50.0% | |
| CAGACGAATGTGGTGACAAG+AGG | - | chr1_1:38008982-38009001 | Msa0021340:CDS | 50.0% | |
| ! | CAGATTTGGGACTTGGAGAC+GGG | - | chr1_1:38009387-38009406 | Msa0021340:intron | 50.0% |
| ! | ACTTGGAGACGGGTGTCATA+TGG | - | chr1_1:38009397-38009416 | Msa0021340:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_1 | gene | 38008832 | 38009584 | 38008832 | ID=Msa0021340;Name=Msa0021340 |
| chr1_1 | mRNA | 38008832 | 38009584 | 38008832 | ID=Msa0021340-mRNA-1;Parent=Msa0021340;Name=Msa0021340-mRNA-1;_AED=0.23;_eAED=0.68;_QI=5|0|0.5|1|0|0|2|0|135 |
| chr1_1 | exon | 38008832 | 38009072 | 38008832 | ID=Msa0021340-mRNA-1:exon:11835;Parent=Msa0021340-mRNA-1 |
| chr1_1 | exon | 38009413 | 38009584 | 38009413 | ID=Msa0021340-mRNA-1:exon:11834;Parent=Msa0021340-mRNA-1 |
| chr1_1 | five_prime_UTR | 38009580 | 38009584 | 38009580 | ID=Msa0021340-mRNA-1:five_prime_utr;Parent=Msa0021340-mRNA-1 |
| chr1_1 | CDS | 38009413 | 38009579 | 38009413 | ID=Msa0021340-mRNA-1:cds;Parent=Msa0021340-mRNA-1 |
| chr1_1 | CDS | 38008832 | 38009072 | 38008832 | ID=Msa0021340-mRNA-1:cds;Parent=Msa0021340-mRNA-1 |
| Gene Sequence |
| Protein sequence |
