AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0614990


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0614990 MtrunA17_Chr4g0042971 88.889 72 8 0 1 72 1 72 9.60e-39 126
Msa0614990 MtrunA17_Chr4g0042971 76.190 84 19 1 4 86 73 156 3.21e-36 119
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 23 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCACTTGGTAGAAACTTATT+TGG 0.193374 4_2:+64200756 None:intergenic
GTTTCTACCAAGTGAGGTTT+TGG 0.234806 4_2:-64200748 Msa0614990:CDS
AGCACACCACCTTCGGATTT+AGG 0.251846 4_2:+64200576 None:intergenic
GAGAAGGTGTCAACTGCTGC+AGG 0.400905 4_2:-64200672 Msa0614990:CDS
TCCGAAGGTGGTGTGCTTGA+TGG 0.441201 4_2:-64200570 Msa0614990:CDS
ACCATCAAGCACACCACCTT+CGG 0.487811 4_2:+64200569 None:intergenic
CTGTAGTTGAGCATGTTGAC+AGG 0.490478 4_2:-64200635 Msa0614990:CDS
TCGGATTTAGGAGGCTCCTC+TGG 0.493494 4_2:+64200588 None:intergenic
GAGTCAGCAGAGACAACTGC+AGG 0.516751 4_2:-64200705 Msa0614990:CDS
GCACTCCCCAAAACCTCACT+TGG 0.541506 4_2:+64200741 None:intergenic
AAAGAAACAATGGCAGAAAG+TGG 0.543749 4_2:-64200783 None:intergenic
AAATAAGTTTCTACCAAGTG+AGG 0.567992 4_2:-64200754 Msa0614990:CDS
GGTGTCAACTGCTGCAGGGG+AGG 0.571411 4_2:-64200667 Msa0614990:CDS
TTTAGGAGGCTCCTCTGGTG+TGG 0.573361 4_2:+64200593 None:intergenic
AGAAGGTGTCAACTGCTGCA+GGG 0.573757 4_2:-64200671 Msa0614990:CDS
GAAGGTGTCAACTGCTGCAG+GGG 0.582580 4_2:-64200670 Msa0614990:CDS
ACACCACCTTCGGATTTAGG+AGG 0.610944 4_2:+64200579 None:intergenic
TGCAGGGAAACAATCTGAGA+AGG 0.618025 4_2:-64200688 Msa0614990:CDS
GAGGAGCCTCCTAAATCCGA+AGG 0.645422 4_2:-64200585 Msa0614990:CDS
TCATGTTCATTCCACACCAG+AGG 0.674914 4_2:-64200604 Msa0614990:CDS
GAGCCTCCTAAATCCGAAGG+TGG 0.678300 4_2:-64200582 Msa0614990:CDS
AGTCAGCAGAGACAACTGCA+GGG 0.685909 4_2:-64200704 Msa0614990:CDS
TGTAGTTGAGCATGTTGACA+GGG 0.688044 4_2:-64200634 Msa0614990:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TCACTTGGTAGAAACTTATT+TGG + chr4_2:64200557-64200576 None:intergenic 30.0%
AAATAAGTTTCTACCAAGTG+AGG - chr4_2:64200556-64200575 Msa0614990:CDS 30.0%
! TTTCTACCAAGTGAGGTTTT+GGG - chr4_2:64200563-64200582 Msa0614990:CDS 35.0%
! GATGGTGTTAAAAAAGCTGT+TGG - chr4_2:64200758-64200777 Msa0614990:CDS 35.0%
! GTTTCTACCAAGTGAGGTTT+TGG - chr4_2:64200562-64200581 Msa0614990:CDS 40.0%
! TTCTACCAAGTGAGGTTTTG+GGG - chr4_2:64200564-64200583 Msa0614990:CDS 40.0%
TGTAGTTGAGCATGTTGACA+GGG - chr4_2:64200676-64200695 Msa0614990:CDS 40.0%
GGTGTTAAAAAAGCTGTTGG+AGG - chr4_2:64200761-64200780 Msa0614990:CDS 40.0%
TGCAGGGAAACAATCTGAGA+AGG - chr4_2:64200622-64200641 Msa0614990:CDS 45.0%
CTGTAGTTGAGCATGTTGAC+AGG - chr4_2:64200675-64200694 Msa0614990:CDS 45.0%
TCATGTTCATTCCACACCAG+AGG - chr4_2:64200706-64200725 Msa0614990:CDS 45.0%
!!! TGAGGTTTTGGGGAGTGCAA+AGG - chr4_2:64200574-64200593 Msa0614990:CDS 50.0%
!!! GTTTTGGGGAGTGCAAAGGT+GGG - chr4_2:64200578-64200597 Msa0614990:CDS 50.0%
AGTCAGCAGAGACAACTGCA+GGG - chr4_2:64200606-64200625 Msa0614990:CDS 50.0%
!! AGAAGGTGTCAACTGCTGCA+GGG - chr4_2:64200639-64200658 Msa0614990:CDS 50.0%
ACACCACCTTCGGATTTAGG+AGG + chr4_2:64200734-64200753 None:intergenic 50.0%
AGCACACCACCTTCGGATTT+AGG + chr4_2:64200737-64200756 None:intergenic 50.0%
ACCATCAAGCACACCACCTT+CGG + chr4_2:64200744-64200763 None:intergenic 50.0%
GCACTCCCCAAAACCTCACT+TGG + chr4_2:64200572-64200591 None:intergenic 55.0%
!!! GGTTTTGGGGAGTGCAAAGG+TGG - chr4_2:64200577-64200596 Msa0614990:CDS 55.0%
GAGTCAGCAGAGACAACTGC+AGG - chr4_2:64200605-64200624 Msa0614990:CDS 55.0%
!! GAGAAGGTGTCAACTGCTGC+AGG - chr4_2:64200638-64200657 Msa0614990:CDS 55.0%
!! GAAGGTGTCAACTGCTGCAG+GGG - chr4_2:64200640-64200659 Msa0614990:CDS 55.0%
TTTAGGAGGCTCCTCTGGTG+TGG + chr4_2:64200720-64200739 None:intergenic 55.0%
TCGGATTTAGGAGGCTCCTC+TGG + chr4_2:64200725-64200744 None:intergenic 55.0%
GAGGAGCCTCCTAAATCCGA+AGG - chr4_2:64200725-64200744 Msa0614990:CDS 55.0%
GAGCCTCCTAAATCCGAAGG+TGG - chr4_2:64200728-64200747 Msa0614990:CDS 55.0%
TCCGAAGGTGGTGTGCTTGA+TGG - chr4_2:64200740-64200759 Msa0614990:CDS 55.0%
GGTGTCAACTGCTGCAGGGG+AGG - chr4_2:64200643-64200662 Msa0614990:CDS 65.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 64200536 64200796 64200536 ID=Msa0614990;Name=Msa0614990
chr4_2 mRNA 64200536 64200796 64200536 ID=Msa0614990-mRNA-1;Parent=Msa0614990;Name=Msa0614990-mRNA-1;_AED=0.45;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|86
chr4_2 exon 64200536 64200796 64200536 ID=Msa0614990-mRNA-1:exon:18028;Parent=Msa0614990-mRNA-1
chr4_2 CDS 64200536 64200796 64200536 ID=Msa0614990-mRNA-1:cds;Parent=Msa0614990-mRNA-1
Gene Sequence

>Msa0614990

ATGGCAGAAAGTGGAGAACCAAATAAGTTTCTACCAAGTGAGGTTTTGGGGAGTGCAAAGGTGGGAGCAGAGTCAGCAGAGACAACTGCAGGGAAACAATCTGAGAAGGTGTCAACTGCTGCAGGGGAGGCTAAGAAGACTGTAGTTGAGCATGTTGACAGGGCTGCTGATCATGTTCATTCCACACCAGAGGAGCCTCCTAAATCCGAAGGTGGTGTGCTTGATGGTGTTAAAAAAGCTGTTGGAGGCTTATTCAAATGA

Protein sequence

>Msa0614990

MAESGEPNKFLPSEVLGSAKVGAESAETTAGKQSEKVSTAAGEAKKTVVEHVDRAADHVHSTPEEPPKSEGGVLDGVKKAVGGLFK*