AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0256330


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0256330 MtrunA17_Chr2g0317561 99.306 144 1 0 21 164 1 144 2.84e-103 292
Msa0256330 MtrunA17_Chr1g0197981 99.306 144 1 0 21 164 1 144 4.71e-103 291
Msa0256330 MtrunA17_Chr2g0282251 67.606 142 46 0 22 163 3 144 4.87e-67 201
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0256330 AT1G53035.1 61.806 144 55 0 21 164 7 150 9.19e-63 190
Msa0256330 AT1G53035.2 61.111 144 56 0 21 164 7 150 4.42e-62 189
Msa0256330 AT3G15358.1 60.417 144 57 0 21 164 1 144 3.87e-60 183

Find 35 sgRNAs with CRISPR-Local

Find 98 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTTGCTTTGGCTATTTCT+TGG 0.186754 2_2:-51066977 Msa0256330:CDS
GAAGAAAGCAATTGAAATTA+TGG 0.218565 2_2:+51067695 None:intergenic
TTAAGACTTCAAAGTGGTTT+TGG 0.231225 2_2:-51067374 Msa0256330:CDS
GGATTATTGTTGTTCACAAT+TGG 0.247863 2_2:-51067161 Msa0256330:CDS
AAAGTATGGAGATTGGAATT+TGG 0.289357 2_2:-51067612 Msa0256330:exon
CAAACATCAGATTCCTTCTC+TGG 0.317012 2_2:-51067182 Msa0256330:CDS
TCTGAAGAGGTACATGTCTT+AGG 0.336826 2_2:-51067266 Msa0256330:CDS
GGTTGCTTGTGTTAAGGATA+TGG 0.337831 2_2:-51067123 Msa0256330:CDS
TCTTGCTTTGGCTATTTCTT+GGG 0.340551 2_2:-51066976 Msa0256330:CDS
GATTATTGTTGTTCACAATT+GGG 0.349528 2_2:-51067160 Msa0256330:CDS
TTCATATTTCAATGGCAGTT+TGG 0.351079 2_2:-51067064 Msa0256330:CDS
ATTGGAATTTGGATGGTAAC+AGG 0.356802 2_2:-51067601 Msa0256330:intron
AATTGGGTTTCTTGTGTTCA+TGG 0.359752 2_2:-51067144 Msa0256330:CDS
TGTTGTTGGGGATCTTGCTT+TGG 0.362898 2_2:-51066988 Msa0256330:CDS
ATTGGCCTAGGCATGTTGTT+GGG 0.364725 2_2:-51067001 Msa0256330:CDS
TTTAACATGTGGATCATTGA+TGG 0.365683 2_2:-51067297 Msa0256330:CDS
AGGTAGCAACCACAAAAGTA+TGG 0.392478 2_2:-51067626 Msa0256330:exon
TTTCAAAGTTTCTTTGCTAA+AGG 0.405302 2_2:-51067098 Msa0256330:CDS
TTGAAGAACTTGCTTGTGAT+TGG 0.405372 2_2:-51067019 Msa0256330:CDS
ATTGCTTAGTTCAACAAGAA+AGG 0.440472 2_2:-51067646 Msa0256330:five_prime_UTR
GTTCATGGTTGCTTGTGTTA+AGG 0.446942 2_2:-51067129 Msa0256330:CDS
TGTGTTTCTTCATATTTCAA+TGG 0.458849 2_2:-51067072 Msa0256330:CDS
AACTTGCTTGTGATTGGCCT+AGG 0.494396 2_2:-51067013 Msa0256330:CDS
GATTGGCCTAGGCATGTTGT+TGG 0.498297 2_2:-51067002 Msa0256330:CDS
AACCACAAAAGTATGGAGAT+TGG 0.499882 2_2:-51067619 Msa0256330:exon
TATGGAGATTGGAATTTGGA+TGG 0.527537 2_2:-51067608 Msa0256330:CDS
TTGGCCTAGGCATGTTGTTG+GGG 0.550117 2_2:-51067000 Msa0256330:CDS
AAGACAGCAATAAAGCTACA+AGG 0.572726 2_2:-51067233 Msa0256330:CDS
AGATCCCCAACAACATGCCT+AGG 0.575697 2_2:+51066996 None:intergenic
TCAACAATAGCTTTAACATG+TGG 0.588889 2_2:-51067308 Msa0256330:CDS
GAGGTACATGTCTTAGGACA+TGG 0.605633 2_2:-51067260 Msa0256330:CDS
GAACAACAATAATCCAGAGA+AGG 0.611024 2_2:+51067169 None:intergenic
GAAACTTTAAGACTTCAAAG+TGG 0.622567 2_2:-51067380 Msa0256330:CDS
TGAATTAATAACTGATCATG+TGG 0.652499 2_2:+51067203 None:intergenic
AGACAGCAATAAAGCTACAA+GGG 0.673413 2_2:-51067232 Msa0256330:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! CACAAGTATTTGTAATTCAA+AGG - chr2_2:51067161-51067180 Msa0256330:CDS 25.0%
!! ATGAAATTTGAAGTGTTATG+AGG - chr2_2:51067220-51067239 Msa0256330:CDS 25.0%
! TGAATTAATAACTGATCATG+TGG + chr2_2:51067429-51067448 None:intergenic 25.0%
!! GATTATTGTTGTTCACAATT+GGG - chr2_2:51067469-51067488 Msa0256330:intron 25.0%
! TTTCAAAGTTTCTTTGCTAA+AGG - chr2_2:51067531-51067550 Msa0256330:intron 25.0%
! TGTGTTTCTTCATATTTCAA+TGG - chr2_2:51067557-51067576 Msa0256330:intron 25.0%
!! TTTGGAGATTTTACTTTGTA+GGG - chr2_2:51067583-51067602 Msa0256330:intron 25.0%
!!! TGTTTTTTCTTGTGTTTATG+TGG - chr2_2:51067676-51067695 Msa0256330:five_prime_UTR 25.0%
! CACAAGTATTTGTAATTCAA+AGG - chr2_2:51067161-51067180 Msa0256330:CDS 25.0%
!! ATGAAATTTGAAGTGTTATG+AGG - chr2_2:51067220-51067239 Msa0256330:CDS 25.0%
! TGAATTAATAACTGATCATG+TGG + chr2_2:51067429-51067448 None:intergenic 25.0%
!! GATTATTGTTGTTCACAATT+GGG - chr2_2:51067469-51067488 Msa0256330:intron 25.0%
! TTTCAAAGTTTCTTTGCTAA+AGG - chr2_2:51067531-51067550 Msa0256330:intron 25.0%
! TGTGTTTCTTCATATTTCAA+TGG - chr2_2:51067557-51067576 Msa0256330:intron 25.0%
!! TTTGGAGATTTTACTTTGTA+GGG - chr2_2:51067583-51067602 Msa0256330:intron 25.0%
!!! TGTTTTTTCTTGTGTTTATG+TGG - chr2_2:51067676-51067695 Msa0256330:five_prime_UTR 25.0%
ATTGCTTAGTTCAACAAGAA+AGG - chr2_2:51066983-51067002 Msa0256330:CDS 30.0%
AAAGTATGGAGATTGGAATT+TGG - chr2_2:51067017-51067036 Msa0256330:CDS 30.0%
!! GAAACTTTAAGACTTCAAAG+TGG - chr2_2:51067249-51067268 Msa0256330:CDS 30.0%
! TTAAGACTTCAAAGTGGTTT+TGG - chr2_2:51067255-51067274 Msa0256330:CDS 30.0%
!!! TCAAAGTGGTTTTGGAAAAA+TGG - chr2_2:51067263-51067282 Msa0256330:CDS 30.0%
!!! CAAAGTGGTTTTGGAAAAAT+GGG - chr2_2:51067264-51067283 Msa0256330:CDS 30.0%
!! TCAACAATAGCTTTAACATG+TGG - chr2_2:51067321-51067340 Msa0256330:CDS 30.0%
TTTAACATGTGGATCATTGA+TGG - chr2_2:51067332-51067351 Msa0256330:CDS 30.0%
! GGATTATTGTTGTTCACAAT+TGG - chr2_2:51067468-51067487 Msa0256330:intron 30.0%
TTCATATTTCAATGGCAGTT+TGG - chr2_2:51067565-51067584 Msa0256330:intron 30.0%
! GTTTGGAGATTTTACTTTGT+AGG - chr2_2:51067582-51067601 Msa0256330:intron 30.0%
! TTTTTCTTGTGTTTATGTGG+AGG - chr2_2:51067679-51067698 Msa0256330:five_prime_UTR 30.0%
! TTTTCTTGTGTTTATGTGGA+GGG - chr2_2:51067680-51067699 Msa0256330:five_prime_UTR 30.0%
ATTGCTTAGTTCAACAAGAA+AGG - chr2_2:51066983-51067002 Msa0256330:CDS 30.0%
AAAGTATGGAGATTGGAATT+TGG - chr2_2:51067017-51067036 Msa0256330:CDS 30.0%
!! GAAACTTTAAGACTTCAAAG+TGG - chr2_2:51067249-51067268 Msa0256330:CDS 30.0%
! TTAAGACTTCAAAGTGGTTT+TGG - chr2_2:51067255-51067274 Msa0256330:CDS 30.0%
!!! TCAAAGTGGTTTTGGAAAAA+TGG - chr2_2:51067263-51067282 Msa0256330:CDS 30.0%
!!! CAAAGTGGTTTTGGAAAAAT+GGG - chr2_2:51067264-51067283 Msa0256330:CDS 30.0%
!! TCAACAATAGCTTTAACATG+TGG - chr2_2:51067321-51067340 Msa0256330:CDS 30.0%
TTTAACATGTGGATCATTGA+TGG - chr2_2:51067332-51067351 Msa0256330:CDS 30.0%
! GGATTATTGTTGTTCACAAT+TGG - chr2_2:51067468-51067487 Msa0256330:intron 30.0%
TTCATATTTCAATGGCAGTT+TGG - chr2_2:51067565-51067584 Msa0256330:intron 30.0%
! GTTTGGAGATTTTACTTTGT+AGG - chr2_2:51067582-51067601 Msa0256330:intron 30.0%
! TTTTTCTTGTGTTTATGTGG+AGG - chr2_2:51067679-51067698 Msa0256330:five_prime_UTR 30.0%
! TTTTCTTGTGTTTATGTGGA+GGG - chr2_2:51067680-51067699 Msa0256330:five_prime_UTR 30.0%
! AACCACAAAAGTATGGAGAT+TGG - chr2_2:51067010-51067029 Msa0256330:CDS 35.0%
! TTCCAATCTCCATACTTTTG+TGG + chr2_2:51067015-51067034 None:intergenic 35.0%
TATGGAGATTGGAATTTGGA+TGG - chr2_2:51067021-51067040 Msa0256330:CDS 35.0%
ATTGGAATTTGGATGGTAAC+AGG - chr2_2:51067028-51067047 Msa0256330:CDS 35.0%
AATTTGGATGGTAACAGGTA+TGG - chr2_2:51067033-51067052 Msa0256330:CDS 35.0%
AAAAGGAGATGAATCAGAAG+AGG + chr2_2:51067130-51067149 None:intergenic 35.0%
CTTGTGAAAGAAGTCTGAAA+AGG + chr2_2:51067147-51067166 None:intergenic 35.0%
!!! GATGGTTTTTTACTCTGAAG+AGG - chr2_2:51067350-51067369 Msa0256330:CDS 35.0%
AAGACAGCAATAAAGCTACA+AGG - chr2_2:51067396-51067415 Msa0256330:intron 35.0%
AGACAGCAATAAAGCTACAA+GGG - chr2_2:51067397-51067416 Msa0256330:intron 35.0%
GAACAACAATAATCCAGAGA+AGG + chr2_2:51067463-51067482 None:intergenic 35.0%
AATTGGGTTTCTTGTGTTCA+TGG - chr2_2:51067485-51067504 Msa0256330:intron 35.0%
! GAGATTTTACTTTGTAGGGA+AGG - chr2_2:51067587-51067606 Msa0256330:intron 35.0%
! TTGAAGAACTTGCTTGTGAT+TGG - chr2_2:51067610-51067629 Msa0256330:CDS 35.0%
!! ATCTTGCTTTGGCTATTTCT+TGG - chr2_2:51067652-51067671 Msa0256330:five_prime_UTR 35.0%
!! TCTTGCTTTGGCTATTTCTT+GGG - chr2_2:51067653-51067672 Msa0256330:five_prime_UTR 35.0%
! AACCACAAAAGTATGGAGAT+TGG - chr2_2:51067010-51067029 Msa0256330:CDS 35.0%
! TTCCAATCTCCATACTTTTG+TGG + chr2_2:51067015-51067034 None:intergenic 35.0%
TATGGAGATTGGAATTTGGA+TGG - chr2_2:51067021-51067040 Msa0256330:CDS 35.0%
ATTGGAATTTGGATGGTAAC+AGG - chr2_2:51067028-51067047 Msa0256330:CDS 35.0%
AATTTGGATGGTAACAGGTA+TGG - chr2_2:51067033-51067052 Msa0256330:CDS 35.0%
AAAAGGAGATGAATCAGAAG+AGG + chr2_2:51067130-51067149 None:intergenic 35.0%
CTTGTGAAAGAAGTCTGAAA+AGG + chr2_2:51067147-51067166 None:intergenic 35.0%
!!! GATGGTTTTTTACTCTGAAG+AGG - chr2_2:51067350-51067369 Msa0256330:CDS 35.0%
AAGACAGCAATAAAGCTACA+AGG - chr2_2:51067396-51067415 Msa0256330:intron 35.0%
AGACAGCAATAAAGCTACAA+GGG - chr2_2:51067397-51067416 Msa0256330:intron 35.0%
GAACAACAATAATCCAGAGA+AGG + chr2_2:51067463-51067482 None:intergenic 35.0%
AATTGGGTTTCTTGTGTTCA+TGG - chr2_2:51067485-51067504 Msa0256330:intron 35.0%
! GAGATTTTACTTTGTAGGGA+AGG - chr2_2:51067587-51067606 Msa0256330:intron 35.0%
! TTGAAGAACTTGCTTGTGAT+TGG - chr2_2:51067610-51067629 Msa0256330:CDS 35.0%
!! ATCTTGCTTTGGCTATTTCT+TGG - chr2_2:51067652-51067671 Msa0256330:five_prime_UTR 35.0%
!! TCTTGCTTTGGCTATTTCTT+GGG - chr2_2:51067653-51067672 Msa0256330:five_prime_UTR 35.0%
AGGTAGCAACCACAAAAGTA+TGG - chr2_2:51067003-51067022 Msa0256330:CDS 40.0%
! TCTGAAGAGGTACATGTCTT+AGG - chr2_2:51067363-51067382 Msa0256330:CDS 40.0%
CAAACATCAGATTCCTTCTC+TGG - chr2_2:51067447-51067466 Msa0256330:intron 40.0%
GTTCATGGTTGCTTGTGTTA+AGG - chr2_2:51067500-51067519 Msa0256330:intron 40.0%
GGTTGCTTGTGTTAAGGATA+TGG - chr2_2:51067506-51067525 Msa0256330:intron 40.0%
AGGTAGCAACCACAAAAGTA+TGG - chr2_2:51067003-51067022 Msa0256330:CDS 40.0%
! TCTGAAGAGGTACATGTCTT+AGG - chr2_2:51067363-51067382 Msa0256330:CDS 40.0%
CAAACATCAGATTCCTTCTC+TGG - chr2_2:51067447-51067466 Msa0256330:intron 40.0%
GTTCATGGTTGCTTGTGTTA+AGG - chr2_2:51067500-51067519 Msa0256330:intron 40.0%
GGTTGCTTGTGTTAAGGATA+TGG - chr2_2:51067506-51067525 Msa0256330:intron 40.0%
GAGGTACATGTCTTAGGACA+TGG - chr2_2:51067369-51067388 Msa0256330:CDS 45.0%
!! AACTTGCTTGTGATTGGCCT+AGG - chr2_2:51067616-51067635 Msa0256330:exon 45.0%
! ATTGGCCTAGGCATGTTGTT+GGG - chr2_2:51067628-51067647 Msa0256330:exon 45.0%
TGTTGTTGGGGATCTTGCTT+TGG - chr2_2:51067641-51067660 Msa0256330:five_prime_UTR 45.0%
GAGGTACATGTCTTAGGACA+TGG - chr2_2:51067369-51067388 Msa0256330:CDS 45.0%
!! AACTTGCTTGTGATTGGCCT+AGG - chr2_2:51067616-51067635 Msa0256330:exon 45.0%
! ATTGGCCTAGGCATGTTGTT+GGG - chr2_2:51067628-51067647 Msa0256330:exon 45.0%
TGTTGTTGGGGATCTTGCTT+TGG - chr2_2:51067641-51067660 Msa0256330:five_prime_UTR 45.0%
!! GATTGGCCTAGGCATGTTGT+TGG - chr2_2:51067627-51067646 Msa0256330:exon 50.0%
TTGGCCTAGGCATGTTGTTG+GGG - chr2_2:51067629-51067648 Msa0256330:exon 50.0%
AGATCCCCAACAACATGCCT+AGG + chr2_2:51067636-51067655 None:intergenic 50.0%
!! GATTGGCCTAGGCATGTTGT+TGG - chr2_2:51067627-51067646 Msa0256330:exon 50.0%
TTGGCCTAGGCATGTTGTTG+GGG - chr2_2:51067629-51067648 Msa0256330:exon 50.0%
AGATCCCCAACAACATGCCT+AGG + chr2_2:51067636-51067655 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 51066935 51067716 51066935 ID=Msa0256330;Name=Msa0256330
chr2_2 mRNA 51066935 51067716 51066935 ID=Msa0256330-mRNA-1;Parent=Msa0256330;Name=Msa0256330-mRNA-1;_AED=0.16;_eAED=0.21;_QI=87|0|0.5|1|0|0|2|0|164
chr2_2 exon 51066935 51067401 51066935 ID=Msa0256330-mRNA-1:exon:15375;Parent=Msa0256330-mRNA-1
chr2_2 exon 51067602 51067716 51067602 ID=Msa0256330-mRNA-1:exon:15374;Parent=Msa0256330-mRNA-1
chr2_2 five_prime_UTR 51067630 51067716 51067630 ID=Msa0256330-mRNA-1:five_prime_utr;Parent=Msa0256330-mRNA-1
chr2_2 CDS 51067602 51067629 51067602 ID=Msa0256330-mRNA-1:cds;Parent=Msa0256330-mRNA-1
chr2_2 CDS 51066935 51067401 51066935 ID=Msa0256330-mRNA-1:cds;Parent=Msa0256330-mRNA-1
Gene Sequence

>Msa0256330

ATGGAGATTGGAATTTGGATGGTAACAGAAACTTTAAGACTTCAAAGTGGTTTTGGAAAAATGGGTTCATCTTGTTTTTTCTTAATATGTGTTCTTCATTCAACAATAGCTTTAACATGTGGATCATTGATGGTTTTTTACTCTGAAGAGGTACATGTCTTAGGACATGGAACAAAGACAGCAATAAAGCTACAAGGGTCAACACCACATGATCAGTTATTAATTCAAACATCAGATTCCTTCTCTGGATTATTGTTGTTCACAATTGGGTTTCTTGTGTTCATGGTTGCTTGTGTTAAGGATATGGAGTTTCAAAGTTTCTTTGCTAAAGGATGTGTGTTTCTTCATATTTCAATGGCAGTTTGGAGATTTTACTTTGTAGGGAAGGTTGAAGAACTTGCTTGTGATTGGCCTAGGCATGTTGTTGGGGATCTTGCTTTGGCTATTTCTTGGGTGTTTTTTCTTGTGTTTATGTGGAGGGAGAAATATGATTAG

Protein sequence

>Msa0256330

MEIGIWMVTETLRLQSGFGKMGSSCFFLICVLHSTIALTCGSLMVFYSEEVHVLGHGTKTAIKLQGSTPHDQLLIQTSDSFSGLLLFTIGFLVFMVACVKDMEFQSFFAKGCVFLHISMAVWRFYFVGKVEELACDWPRHVVGDLALAISWVFFLVFMWREKYD*