AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0579370


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0579370 MtrunA17_Chr4g0042971 88.889 72 8 0 1 72 1 72 9.60e-39 126
Msa0579370 MtrunA17_Chr4g0042971 76.190 84 19 1 4 86 73 156 3.21e-36 119
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 23 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCACTTGGTAGAAACTTATT+TGG 0.193374 4_1:+84014849 None:intergenic
GTTTCTACCAAGTGAGGTTT+TGG 0.234806 4_1:-84014841 Msa0579370:CDS
AGCACACCACCTTCGGATTT+AGG 0.251846 4_1:+84014669 None:intergenic
GAGAAGGTGTCAACTGCTGC+AGG 0.400905 4_1:-84014765 Msa0579370:CDS
TCCGAAGGTGGTGTGCTTGA+TGG 0.441201 4_1:-84014663 Msa0579370:CDS
ACCATCAAGCACACCACCTT+CGG 0.487811 4_1:+84014662 None:intergenic
CTGTAGTTGAGCATGTTGAC+AGG 0.490478 4_1:-84014728 Msa0579370:CDS
TCGGATTTAGGAGGCTCCTC+TGG 0.493494 4_1:+84014681 None:intergenic
GAGTCAGCAGAGACAACTGC+AGG 0.516751 4_1:-84014798 Msa0579370:CDS
GCACTCCCCAAAACCTCACT+TGG 0.541506 4_1:+84014834 None:intergenic
AAAGAAACAATGGCAGAAAG+TGG 0.544848 4_1:-84014876 None:intergenic
AAATAAGTTTCTACCAAGTG+AGG 0.567992 4_1:-84014847 Msa0579370:CDS
GGTGTCAACTGCTGCAGGGG+AGG 0.571411 4_1:-84014760 Msa0579370:CDS
TTTAGGAGGCTCCTCTGGTG+TGG 0.573361 4_1:+84014686 None:intergenic
AGAAGGTGTCAACTGCTGCA+GGG 0.573757 4_1:-84014764 Msa0579370:CDS
GAAGGTGTCAACTGCTGCAG+GGG 0.582580 4_1:-84014763 Msa0579370:CDS
ACACCACCTTCGGATTTAGG+AGG 0.610944 4_1:+84014672 None:intergenic
TGCAGGGAAACAATCTGAGA+AGG 0.618025 4_1:-84014781 Msa0579370:CDS
GAGGAGCCTCCTAAATCCGA+AGG 0.645422 4_1:-84014678 Msa0579370:CDS
TCATGTTCATTCCACACCAG+AGG 0.674914 4_1:-84014697 Msa0579370:CDS
GAGCCTCCTAAATCCGAAGG+TGG 0.678300 4_1:-84014675 Msa0579370:CDS
AGTCAGCAGAGACAACTGCA+GGG 0.685909 4_1:-84014797 Msa0579370:CDS
TGTAGTTGAGCATGTTGACA+GGG 0.688044 4_1:-84014727 Msa0579370:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TCACTTGGTAGAAACTTATT+TGG + chr4_1:84014650-84014669 None:intergenic 30.0%
AAATAAGTTTCTACCAAGTG+AGG - chr4_1:84014649-84014668 Msa0579370:CDS 30.0%
! TTTCTACCAAGTGAGGTTTT+GGG - chr4_1:84014656-84014675 Msa0579370:CDS 35.0%
! GATGGTGTTAAAAAAGCTGT+TGG - chr4_1:84014851-84014870 Msa0579370:CDS 35.0%
! GTTTCTACCAAGTGAGGTTT+TGG - chr4_1:84014655-84014674 Msa0579370:CDS 40.0%
! TTCTACCAAGTGAGGTTTTG+GGG - chr4_1:84014657-84014676 Msa0579370:CDS 40.0%
TGTAGTTGAGCATGTTGACA+GGG - chr4_1:84014769-84014788 Msa0579370:CDS 40.0%
GGTGTTAAAAAAGCTGTTGG+AGG - chr4_1:84014854-84014873 Msa0579370:CDS 40.0%
TGCAGGGAAACAATCTGAGA+AGG - chr4_1:84014715-84014734 Msa0579370:CDS 45.0%
CTGTAGTTGAGCATGTTGAC+AGG - chr4_1:84014768-84014787 Msa0579370:CDS 45.0%
TCATGTTCATTCCACACCAG+AGG - chr4_1:84014799-84014818 Msa0579370:CDS 45.0%
!!! TGAGGTTTTGGGGAGTGCAA+AGG - chr4_1:84014667-84014686 Msa0579370:CDS 50.0%
!!! GTTTTGGGGAGTGCAAAGGT+GGG - chr4_1:84014671-84014690 Msa0579370:CDS 50.0%
AGTCAGCAGAGACAACTGCA+GGG - chr4_1:84014699-84014718 Msa0579370:CDS 50.0%
!! AGAAGGTGTCAACTGCTGCA+GGG - chr4_1:84014732-84014751 Msa0579370:CDS 50.0%
ACACCACCTTCGGATTTAGG+AGG + chr4_1:84014827-84014846 None:intergenic 50.0%
AGCACACCACCTTCGGATTT+AGG + chr4_1:84014830-84014849 None:intergenic 50.0%
ACCATCAAGCACACCACCTT+CGG + chr4_1:84014837-84014856 None:intergenic 50.0%
GCACTCCCCAAAACCTCACT+TGG + chr4_1:84014665-84014684 None:intergenic 55.0%
!!! GGTTTTGGGGAGTGCAAAGG+TGG - chr4_1:84014670-84014689 Msa0579370:CDS 55.0%
GAGTCAGCAGAGACAACTGC+AGG - chr4_1:84014698-84014717 Msa0579370:CDS 55.0%
!! GAGAAGGTGTCAACTGCTGC+AGG - chr4_1:84014731-84014750 Msa0579370:CDS 55.0%
!! GAAGGTGTCAACTGCTGCAG+GGG - chr4_1:84014733-84014752 Msa0579370:CDS 55.0%
TTTAGGAGGCTCCTCTGGTG+TGG + chr4_1:84014813-84014832 None:intergenic 55.0%
TCGGATTTAGGAGGCTCCTC+TGG + chr4_1:84014818-84014837 None:intergenic 55.0%
GAGGAGCCTCCTAAATCCGA+AGG - chr4_1:84014818-84014837 Msa0579370:CDS 55.0%
GAGCCTCCTAAATCCGAAGG+TGG - chr4_1:84014821-84014840 Msa0579370:CDS 55.0%
TCCGAAGGTGGTGTGCTTGA+TGG - chr4_1:84014833-84014852 Msa0579370:CDS 55.0%
GGTGTCAACTGCTGCAGGGG+AGG - chr4_1:84014736-84014755 Msa0579370:CDS 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 84014629 84014889 84014629 ID=Msa0579370;Name=Msa0579370
chr4_1 mRNA 84014629 84014889 84014629 ID=Msa0579370-mRNA-1;Parent=Msa0579370;Name=Msa0579370-mRNA-1;_AED=0.51;_eAED=0.52;_QI=0|-1|0|1|-1|1|1|0|86
chr4_1 exon 84014629 84014889 84014629 ID=Msa0579370-mRNA-1:exon:30787;Parent=Msa0579370-mRNA-1
chr4_1 CDS 84014629 84014889 84014629 ID=Msa0579370-mRNA-1:cds;Parent=Msa0579370-mRNA-1
Gene Sequence

>Msa0579370

ATGGCAGAAAGTGGAGAACCAAATAAGTTTCTACCAAGTGAGGTTTTGGGGAGTGCAAAGGTGGGAGCAGAGTCAGCAGAGACAACTGCAGGGAAACAATCTGAGAAGGTGTCAACTGCTGCAGGGGAGGCTAAGAAGACTGTAGTTGAGCATGTTGACAGGGCTGCTGATCATGTTCATTCCACACCAGAGGAGCCTCCTAAATCCGAAGGTGGTGTGCTTGATGGTGTTAAAAAAGCTGTTGGAGGCTTATTCAAATGA

Protein sequence

>Msa0579370

MAESGEPNKFLPSEVLGSAKVGAESAETTAGKQSEKVSTAAGEAKKTVVEHVDRAADHVHSTPEEPPKSEGGVLDGVKKAVGGLFK*