Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0051780 | AES60671.1 | 59.223 | 103 | 42 | 0 | 92 | 194 | 1 | 103 | 1.40e-33 | 126 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0051780 | G7IA54 | 59.223 | 103 | 42 | 0 | 92 | 194 | 1 | 103 | 6.70e-34 | 126 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0004870 | Msa0051780 | 0.986857 | 3.279680e-168 | -8.615850e-47 |
| Msa0004880 | Msa0051780 | 0.876358 | 1.487625e-68 | -8.615850e-47 |
| Msa0051780 | Msa0051790 | 0.873489 | 1.413706e-67 | -8.615850e-47 |
| Msa0051780 | Msa0191040 | 0.817438 | 3.297385e-52 | -8.615850e-47 |
| Msa0051780 | Msa0233340 | 0.818571 | 1.828402e-52 | -8.615850e-47 |
| Msa0051780 | Msa0266450 | 0.803509 | 3.370045e-49 | -8.615850e-47 |
| Msa0051780 | Msa0290010 | 0.807815 | 4.203646e-50 | -8.615850e-47 |
| Msa0051780 | Msa0311690 | 0.820944 | 5.247037e-53 | -8.615850e-47 |
| Msa0051780 | Msa0484780 | 0.805516 | 1.285754e-49 | -8.615850e-47 |
| Msa0051780 | Msa0531430 | 0.805845 | 1.096568e-49 | -8.615850e-47 |
| Msa0051780 | Msa0534590 | 0.803426 | 3.506321e-49 | -8.615850e-47 |
| Msa0051780 | Msa0631120 | 0.805951 | 1.041557e-49 | -8.615850e-47 |
| Msa0051780 | Msa0666030 | 0.816094 | 6.601645e-52 | -8.615850e-47 |
| Msa0051780 | Msa1172670 | 0.832055 | 1.175535e-55 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0051780 | MtrunA17_Chr7g0233401 | 57.282 | 103 | 44 | 0 | 92 | 194 | 1 | 103 | 4.79e-36 | 123 |
| Msa0051780 | MtrunA17_Chr7g0233391 | 75.472 | 53 | 13 | 0 | 1 | 53 | 191 | 243 | 6.38e-22 | 89.7 |
| Msa0051780 | MtrunA17_Chr2g0307271 | 33.333 | 153 | 94 | 3 | 1 | 149 | 193 | 341 | 1.31e-16 | 76.6 |
| Msa0051780 | MtrunA17_Chr6g0470121 | 44.928 | 69 | 36 | 1 | 1 | 67 | 105 | 173 | 1.37e-13 | 67.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 48 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGTTTCCGCCCTACCGAAAA+TGG | 0.261437 | 1_2:+5893863 | None:intergenic |
| CCCCACCACTATCTTGCCTT+TGG | 0.275407 | 1_2:+5893731 | None:intergenic |
| CAGATGCCAAATGGCTTAAA+AGG | 0.303609 | 1_2:+5893766 | None:intergenic |
| ACACAACAAATCTAGCAAAT+TGG | 0.306026 | 1_2:-5893509 | Msa0051780:CDS |
| CCCACCACTATCTTGCCTTT+GGG | 0.322836 | 1_2:+5893732 | None:intergenic |
| TTGCTACAAGCATTTGTCTT+TGG | 0.323587 | 1_2:+5893319 | None:intergenic |
| AATATCAACAATTGAAAATT+CGG | 0.329798 | 1_2:+5893573 | None:intergenic |
| TGAAGATATGGGTGATAATC+AGG | 0.355872 | 1_2:-5893436 | Msa0051780:CDS |
| GTGTCAGTTCCCAAGGGATT+TGG | 0.362335 | 1_2:+5893528 | None:intergenic |
| GCCTGCGCAAGATGAAGATA+TGG | 0.364425 | 1_2:-5893448 | Msa0051780:CDS |
| GTAAACAGATTCATATGGTT+AGG | 0.366712 | 1_2:-5893614 | Msa0051780:CDS |
| TCATCTTGCATTCCTCCTAT+AGG | 0.406756 | 1_2:+5893402 | None:intergenic |
| TAAACAGATTCATATGGTTA+GGG | 0.421849 | 1_2:-5893613 | Msa0051780:CDS |
| CATATGGTTAGGGATATAGA+TGG | 0.425750 | 1_2:-5893603 | Msa0051780:CDS |
| TAGTTTCATTGCTTGGAAGA+TGG | 0.442772 | 1_2:-5893700 | Msa0051780:CDS |
| GAATGCAAGATGACGATGAT+TGG | 0.446928 | 1_2:-5893392 | Msa0051780:CDS |
| AGTTTCATTGCTTGGAAGAT+GGG | 0.448047 | 1_2:-5893699 | Msa0051780:CDS |
| GATTGATTAGTTTCATTGCT+TGG | 0.450315 | 1_2:-5893707 | Msa0051780:CDS |
| GCGTGGAACGTTGGAGGCTA+AGG | 0.472074 | 1_2:-5893370 | Msa0051780:CDS |
| CAGGAGGAACAACAACCTAT+AGG | 0.482504 | 1_2:-5893417 | Msa0051780:CDS |
| ATTTGTTGTGTCAGTTCCCA+AGG | 0.506460 | 1_2:+5893521 | None:intergenic |
| GAGCCGCCCCAGATGCCAAA+TGG | 0.514528 | 1_2:+5893757 | None:intergenic |
| CGCCCTACCGAAAATGGTGC+AGG | 0.523453 | 1_2:+5893869 | None:intergenic |
| TCTTGCGCAGGCTGCTCTTG+AGG | 0.528212 | 1_2:+5893459 | None:intergenic |
| CCCATATCTTCATCTTGCGC+AGG | 0.536558 | 1_2:+5893447 | None:intergenic |
| CCTGCGCAAGATGAAGATAT+GGG | 0.541191 | 1_2:-5893447 | Msa0051780:CDS |
| TCAACCCAAAGGCAAGATAG+TGG | 0.544870 | 1_2:-5893736 | Msa0051780:CDS |
| AAGCCATTTGGCATCTGGGG+CGG | 0.551498 | 1_2:-5893760 | Msa0051780:CDS |
| CCCAAAGGCAAGATAGTGGT+GGG | 0.553523 | 1_2:-5893732 | Msa0051780:CDS |
| CAAAGGCAAGATAGTGGTGG+GGG | 0.554478 | 1_2:-5893730 | Msa0051780:CDS |
| GGCTGCTCTTGAGGTACATG+TGG | 0.562387 | 1_2:+5893468 | None:intergenic |
| CTGAAGAAAATCACATTGAG+AGG | 0.569791 | 1_2:-5893662 | Msa0051780:CDS |
| TTTAAGCCATTTGGCATCTG+GGG | 0.574619 | 1_2:-5893763 | Msa0051780:CDS |
| TGATTGGCGTGGAACGTTGG+AGG | 0.575574 | 1_2:-5893376 | Msa0051780:CDS |
| TTTGTTGTGTCAGTTCCCAA+GGG | 0.584927 | 1_2:+5893522 | None:intergenic |
| CATTGCTTGGAAGATGGGAG+CGG | 0.585834 | 1_2:-5893694 | Msa0051780:CDS |
| ACCCAAAGGCAAGATAGTGG+TGG | 0.591053 | 1_2:-5893733 | Msa0051780:CDS |
| CGATGATTGGCGTGGAACGT+TGG | 0.592476 | 1_2:-5893379 | Msa0051780:CDS |
| ATATGGTTAGGGATATAGAT+GGG | 0.593840 | 1_2:-5893602 | Msa0051780:CDS |
| TCTGGGGCGGCTCAACCCAA+AGG | 0.596207 | 1_2:-5893747 | Msa0051780:CDS |
| AACAAATGTGCTGTAAACGC+TGG | 0.603099 | 1_2:-5893795 | Msa0051780:CDS |
| TAAGGTTGACAAGATTAATG+AGG | 0.608406 | 1_2:-5893352 | Msa0051780:CDS |
| CCAAAGGCAAGATAGTGGTG+GGG | 0.619095 | 1_2:-5893731 | Msa0051780:CDS |
| GAGGAACAACAACCTATAGG+AGG | 0.638621 | 1_2:-5893414 | Msa0051780:CDS |
| CAAGATGACGATGATTGGCG+TGG | 0.680070 | 1_2:-5893387 | Msa0051780:CDS |
| GGGCGGAAACGGGAACAGTG+AGG | 0.685551 | 1_2:-5893851 | Msa0051780:CDS |
| AGATATGGGTGATAATCAGG+AGG | 0.694136 | 1_2:-5893433 | Msa0051780:CDS |
| AAAGGCAAGATAGTGGTGGG+GGG | 0.727123 | 1_2:-5893729 | Msa0051780:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATATCAACAATTGAAAATT+CGG | + | chr1_2:5893614-5893633 | None:intergenic | 15.0% |
| !!! | TTTTTGTAAACAGATTCATA+TGG | - | chr1_2:5893565-5893584 | Msa0051780:CDS | 20.0% |
| !! | TGATAAGCTATTCATTTTGT+GGG | - | chr1_2:5893358-5893377 | Msa0051780:CDS | 25.0% |
| ! | TAAACAGATTCATATGGTTA+GGG | - | chr1_2:5893571-5893590 | Msa0051780:CDS | 25.0% |
| ! | GTGATAAGCTATTCATTTTG+TGG | - | chr1_2:5893357-5893376 | Msa0051780:CDS | 30.0% |
| !! | GATTGATTAGTTTCATTGCT+TGG | - | chr1_2:5893477-5893496 | Msa0051780:CDS | 30.0% |
| GTAAACAGATTCATATGGTT+AGG | - | chr1_2:5893570-5893589 | Msa0051780:CDS | 30.0% | |
| ATATGGTTAGGGATATAGAT+GGG | - | chr1_2:5893582-5893601 | Msa0051780:CDS | 30.0% | |
| ACACAACAAATCTAGCAAAT+TGG | - | chr1_2:5893675-5893694 | Msa0051780:CDS | 30.0% | |
| TAAGGTTGACAAGATTAATG+AGG | - | chr1_2:5893832-5893851 | Msa0051780:CDS | 30.0% | |
| ! | CTACTACCTTTTAAGCCATT+TGG | - | chr1_2:5893412-5893431 | Msa0051780:CDS | 35.0% |
| ! | TTTTAAGCCATTTGGCATCT+GGG | - | chr1_2:5893420-5893439 | Msa0051780:CDS | 35.0% |
| TAGTTTCATTGCTTGGAAGA+TGG | - | chr1_2:5893484-5893503 | Msa0051780:CDS | 35.0% | |
| AGTTTCATTGCTTGGAAGAT+GGG | - | chr1_2:5893485-5893504 | Msa0051780:CDS | 35.0% | |
| CTGAAGAAAATCACATTGAG+AGG | - | chr1_2:5893522-5893541 | Msa0051780:CDS | 35.0% | |
| CATATGGTTAGGGATATAGA+TGG | - | chr1_2:5893581-5893600 | Msa0051780:CDS | 35.0% | |
| ! | AATTTTGTTGCCAAATCCCT+TGG | - | chr1_2:5893646-5893665 | Msa0051780:CDS | 35.0% |
| ! | ATTTTGTTGCCAAATCCCTT+GGG | - | chr1_2:5893647-5893666 | Msa0051780:CDS | 35.0% |
| TGAAGATATGGGTGATAATC+AGG | - | chr1_2:5893748-5893767 | Msa0051780:CDS | 35.0% | |
| TTGCTACAAGCATTTGTCTT+TGG | + | chr1_2:5893868-5893887 | None:intergenic | 35.0% | |
| !! | GCTATTCATTTTGTGGGCTA+TGG | - | chr1_2:5893364-5893383 | Msa0051780:CDS | 40.0% |
| AACAAATGTGCTGTAAACGC+TGG | - | chr1_2:5893389-5893408 | Msa0051780:CDS | 40.0% | |
| CAGATGCCAAATGGCTTAAA+AGG | + | chr1_2:5893421-5893440 | None:intergenic | 40.0% | |
| ! | CTTTTAAGCCATTTGGCATC+TGG | - | chr1_2:5893419-5893438 | Msa0051780:CDS | 40.0% |
| TTTAAGCCATTTGGCATCTG+GGG | - | chr1_2:5893421-5893440 | Msa0051780:CDS | 40.0% | |
| ! | TTTGTTGTGTCAGTTCCCAA+GGG | + | chr1_2:5893665-5893684 | None:intergenic | 40.0% |
| !! | ATTTGTTGTGTCAGTTCCCA+AGG | + | chr1_2:5893666-5893685 | None:intergenic | 40.0% |
| AGATATGGGTGATAATCAGG+AGG | - | chr1_2:5893751-5893770 | Msa0051780:CDS | 40.0% | |
| TCATCTTGCATTCCTCCTAT+AGG | + | chr1_2:5893785-5893804 | None:intergenic | 40.0% | |
| GAATGCAAGATGACGATGAT+TGG | - | chr1_2:5893792-5893811 | Msa0051780:CDS | 40.0% | |
| TCAACCCAAAGGCAAGATAG+TGG | - | chr1_2:5893448-5893467 | Msa0051780:CDS | 45.0% | |
| CCTGCGCAAGATGAAGATAT+GGG | - | chr1_2:5893737-5893756 | Msa0051780:CDS | 45.0% | |
| CAGGAGGAACAACAACCTAT+AGG | - | chr1_2:5893767-5893786 | Msa0051780:CDS | 45.0% | |
| GAGGAACAACAACCTATAGG+AGG | - | chr1_2:5893770-5893789 | Msa0051780:CDS | 45.0% | |
| ! | TGTCCTGCACCATTTTCGGT+AGG | - | chr1_2:5893312-5893331 | Msa0051780:CDS | 50.0% |
| ! | GTCCTGCACCATTTTCGGTA+GGG | - | chr1_2:5893313-5893332 | Msa0051780:CDS | 50.0% |
| ! | CATTTTCGGTAGGGCGGAAA+CGG | - | chr1_2:5893322-5893341 | Msa0051780:CDS | 50.0% |
| ! | ATTTTCGGTAGGGCGGAAAC+GGG | - | chr1_2:5893323-5893342 | Msa0051780:CDS | 50.0% |
| ! | ACCCAAAGGCAAGATAGTGG+TGG | - | chr1_2:5893451-5893470 | Msa0051780:CDS | 50.0% |
| CCCACCACTATCTTGCCTTT+GGG | + | chr1_2:5893455-5893474 | None:intergenic | 50.0% | |
| ! | CCCAAAGGCAAGATAGTGGT+GGG | - | chr1_2:5893452-5893471 | Msa0051780:CDS | 50.0% |
| ! | CCAAAGGCAAGATAGTGGTG+GGG | - | chr1_2:5893453-5893472 | Msa0051780:CDS | 50.0% |
| ! | CAAAGGCAAGATAGTGGTGG+GGG | - | chr1_2:5893454-5893473 | Msa0051780:CDS | 50.0% |
| ! | AAAGGCAAGATAGTGGTGGG+GGG | - | chr1_2:5893455-5893474 | Msa0051780:CDS | 50.0% |
| CATTGCTTGGAAGATGGGAG+CGG | - | chr1_2:5893490-5893509 | Msa0051780:CDS | 50.0% | |
| GTGTCAGTTCCCAAGGGATT+TGG | + | chr1_2:5893659-5893678 | None:intergenic | 50.0% | |
| GCCTGCGCAAGATGAAGATA+TGG | - | chr1_2:5893736-5893755 | Msa0051780:CDS | 50.0% | |
| CCCATATCTTCATCTTGCGC+AGG | + | chr1_2:5893740-5893759 | None:intergenic | 50.0% | |
| CAAGATGACGATGATTGGCG+TGG | - | chr1_2:5893797-5893816 | Msa0051780:CDS | 50.0% | |
| ! | CTGCACCATTTTCGGTAGGG+CGG | - | chr1_2:5893316-5893335 | Msa0051780:CDS | 55.0% |
| CGTTTCCGCCCTACCGAAAA+TGG | + | chr1_2:5893324-5893343 | None:intergenic | 55.0% | |
| AAGCCATTTGGCATCTGGGG+CGG | - | chr1_2:5893424-5893443 | Msa0051780:CDS | 55.0% | |
| CCCCACCACTATCTTGCCTT+TGG | + | chr1_2:5893456-5893475 | None:intergenic | 55.0% | |
| GGCTGCTCTTGAGGTACATG+TGG | + | chr1_2:5893719-5893738 | None:intergenic | 55.0% | |
| CGATGATTGGCGTGGAACGT+TGG | - | chr1_2:5893805-5893824 | Msa0051780:CDS | 55.0% | |
| TGATTGGCGTGGAACGTTGG+AGG | - | chr1_2:5893808-5893827 | Msa0051780:CDS | 55.0% | |
| CGCCCTACCGAAAATGGTGC+AGG | + | chr1_2:5893318-5893337 | None:intergenic | 60.0% | |
| TCTTGCGCAGGCTGCTCTTG+AGG | + | chr1_2:5893728-5893747 | None:intergenic | 60.0% | |
| GCGTGGAACGTTGGAGGCTA+AGG | - | chr1_2:5893814-5893833 | Msa0051780:CDS | 60.0% | |
| GGGCGGAAACGGGAACAGTG+AGG | - | chr1_2:5893333-5893352 | Msa0051780:CDS | 65.0% | |
| GAGCCGCCCCAGATGCCAAA+TGG | + | chr1_2:5893430-5893449 | None:intergenic | 65.0% | |
| ! | TCTGGGGCGGCTCAACCCAA+AGG | - | chr1_2:5893437-5893456 | Msa0051780:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_2 | gene | 5893311 | 5893895 | 5893311 | ID=Msa0051780;Name=Msa0051780 |
| chr1_2 | mRNA | 5893311 | 5893895 | 5893311 | ID=Msa0051780-mRNA-1;Parent=Msa0051780;Name=Msa0051780-mRNA-1;_AED=0.45;_eAED=0.66;_QI=0|-1|0|1|-1|1|1|0|194 |
| chr1_2 | exon | 5893311 | 5893895 | 5893311 | ID=Msa0051780-mRNA-1:exon:1922;Parent=Msa0051780-mRNA-1 |
| chr1_2 | CDS | 5893311 | 5893895 | 5893311 | ID=Msa0051780-mRNA-1:cds;Parent=Msa0051780-mRNA-1 |
| Gene Sequence |
| Protein sequence |
