Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0004870 | Msa0004880 | 0.886363 | 3.650918e-72 | -8.615850e-47 |
| Msa0004870 | Msa0051780 | 0.986857 | 3.279680e-168 | -8.615850e-47 |
| Msa0004870 | Msa0051790 | 0.874097 | 8.813712e-68 | -8.615850e-47 |
| Msa0004870 | Msa0191040 | 0.822477 | 2.319875e-53 | -8.615850e-47 |
| Msa0004870 | Msa0233340 | 0.823629 | 1.249798e-53 | -8.615850e-47 |
| Msa0004870 | Msa0266450 | 0.804768 | 1.843855e-49 | -8.615850e-47 |
| Msa0004870 | Msa0290010 | 0.809074 | 2.264758e-50 | -8.615850e-47 |
| Msa0004870 | Msa0311690 | 0.826944 | 2.054352e-54 | -8.615850e-47 |
| Msa0004870 | Msa0311700 | 0.807127 | 5.883442e-50 | -8.615850e-47 |
| Msa0004870 | Msa0512890 | 0.801637 | 8.198217e-49 | -8.615850e-47 |
| Msa0004870 | Msa0531430 | 0.809872 | 1.526616e-50 | -8.615850e-47 |
| Msa0004870 | Msa0534590 | 0.810637 | 1.044231e-50 | -8.615850e-47 |
| Msa0004870 | Msa0590800 | 0.804526 | 2.070448e-49 | -8.615850e-47 |
| Msa0004870 | Msa0631120 | 0.810740 | 9.917472e-51 | -8.615850e-47 |
| Msa0004870 | Msa0666030 | 0.817336 | 3.476959e-52 | -8.615850e-47 |
| Msa0004870 | Msa0933230 | 0.803884 | 2.817609e-49 | -8.615850e-47 |
| Msa0004870 | Msa0933310 | 0.801880 | 7.308300e-49 | -8.615850e-47 |
| Msa0004870 | Msa1172670 | 0.831601 | 1.522049e-55 | -8.615850e-47 |
| Msa0004870 | Msa1300870 | 0.811081 | 8.365891e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0004870 | MtrunA17_Chr7g0233401 | 57.282 | 103 | 44 | 0 | 92 | 194 | 1 | 103 | 4.79e-36 | 123 |
| Msa0004870 | MtrunA17_Chr7g0233391 | 75.472 | 53 | 13 | 0 | 1 | 53 | 191 | 243 | 6.38e-22 | 89.7 |
| Msa0004870 | MtrunA17_Chr2g0307271 | 33.333 | 153 | 94 | 3 | 1 | 149 | 193 | 341 | 1.31e-16 | 76.6 |
| Msa0004870 | MtrunA17_Chr6g0470121 | 44.928 | 69 | 36 | 1 | 1 | 67 | 105 | 173 | 1.37e-13 | 67.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 48 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGTTTCCGCCCTACCGAAAA+TGG | 0.261437 | 1_1:+7349241 | None:intergenic |
| CCCCACCACTATCTTGCCTT+TGG | 0.275407 | 1_1:+7349109 | None:intergenic |
| CAGATGCCAAATGGCTTAAA+AGG | 0.303609 | 1_1:+7349144 | None:intergenic |
| ACACAACAAATCTAGCAAAT+TGG | 0.306026 | 1_1:-7348887 | Msa0004870:CDS |
| CCCACCACTATCTTGCCTTT+GGG | 0.322836 | 1_1:+7349110 | None:intergenic |
| TTGCTACAAGCATTTGTCTT+TGG | 0.323587 | 1_1:+7348697 | None:intergenic |
| AATATCAACAATTGAAAATT+CGG | 0.329798 | 1_1:+7348951 | None:intergenic |
| TGAAGATATGGGTGATAATC+AGG | 0.355872 | 1_1:-7348814 | Msa0004870:CDS |
| GTGTCAGTTCCCAAGGGATT+TGG | 0.362335 | 1_1:+7348906 | None:intergenic |
| GCCTGCGCAAGATGAAGATA+TGG | 0.364425 | 1_1:-7348826 | Msa0004870:CDS |
| GTAAACAGATTCATATGGTT+AGG | 0.366712 | 1_1:-7348992 | Msa0004870:CDS |
| TCATCTTGCATTCCTCCTAT+AGG | 0.406756 | 1_1:+7348780 | None:intergenic |
| TAAACAGATTCATATGGTTA+GGG | 0.421849 | 1_1:-7348991 | Msa0004870:CDS |
| CATATGGTTAGGGATATAGA+TGG | 0.425750 | 1_1:-7348981 | Msa0004870:CDS |
| TAGTTTCATTGCTTGGAAGA+TGG | 0.442772 | 1_1:-7349078 | Msa0004870:CDS |
| GAATGCAAGATGACGATGAT+TGG | 0.446928 | 1_1:-7348770 | Msa0004870:CDS |
| AGTTTCATTGCTTGGAAGAT+GGG | 0.448047 | 1_1:-7349077 | Msa0004870:CDS |
| GATTGATTAGTTTCATTGCT+TGG | 0.450315 | 1_1:-7349085 | Msa0004870:CDS |
| GCGTGGAACGTTGGAGGCTA+AGG | 0.472074 | 1_1:-7348748 | Msa0004870:CDS |
| CAGGAGGAACAACAACCTAT+AGG | 0.482504 | 1_1:-7348795 | Msa0004870:CDS |
| ATTTGTTGTGTCAGTTCCCA+AGG | 0.506460 | 1_1:+7348899 | None:intergenic |
| GAGCCGCCCCAGATGCCAAA+TGG | 0.514528 | 1_1:+7349135 | None:intergenic |
| CGCCCTACCGAAAATGGTGC+AGG | 0.523453 | 1_1:+7349247 | None:intergenic |
| TCTTGCGCAGGCTGCTCTTG+AGG | 0.528212 | 1_1:+7348837 | None:intergenic |
| CCCATATCTTCATCTTGCGC+AGG | 0.536558 | 1_1:+7348825 | None:intergenic |
| CCTGCGCAAGATGAAGATAT+GGG | 0.541191 | 1_1:-7348825 | Msa0004870:CDS |
| TCAACCCAAAGGCAAGATAG+TGG | 0.544870 | 1_1:-7349114 | Msa0004870:CDS |
| AAGCCATTTGGCATCTGGGG+CGG | 0.551498 | 1_1:-7349138 | Msa0004870:CDS |
| CCCAAAGGCAAGATAGTGGT+GGG | 0.553523 | 1_1:-7349110 | Msa0004870:CDS |
| CAAAGGCAAGATAGTGGTGG+GGG | 0.554478 | 1_1:-7349108 | Msa0004870:CDS |
| GGCTGCTCTTGAGGTACATG+TGG | 0.562387 | 1_1:+7348846 | None:intergenic |
| CTGAAGAAAATCACATTGAG+AGG | 0.569791 | 1_1:-7349040 | Msa0004870:CDS |
| TTTAAGCCATTTGGCATCTG+GGG | 0.574619 | 1_1:-7349141 | Msa0004870:CDS |
| TGATTGGCGTGGAACGTTGG+AGG | 0.575574 | 1_1:-7348754 | Msa0004870:CDS |
| TTTGTTGTGTCAGTTCCCAA+GGG | 0.584927 | 1_1:+7348900 | None:intergenic |
| CATTGCTTGGAAGATGGGAG+CGG | 0.585834 | 1_1:-7349072 | Msa0004870:CDS |
| ACCCAAAGGCAAGATAGTGG+TGG | 0.591053 | 1_1:-7349111 | Msa0004870:CDS |
| CGATGATTGGCGTGGAACGT+TGG | 0.592476 | 1_1:-7348757 | Msa0004870:CDS |
| ATATGGTTAGGGATATAGAT+GGG | 0.593840 | 1_1:-7348980 | Msa0004870:CDS |
| TCTGGGGCGGCTCAACCCAA+AGG | 0.596207 | 1_1:-7349125 | Msa0004870:CDS |
| AACAAATGTGCTGTAAACGC+TGG | 0.603099 | 1_1:-7349173 | Msa0004870:CDS |
| TAAGGTTGACAAGATTAATG+AGG | 0.608406 | 1_1:-7348730 | Msa0004870:CDS |
| CCAAAGGCAAGATAGTGGTG+GGG | 0.619095 | 1_1:-7349109 | Msa0004870:CDS |
| GAGGAACAACAACCTATAGG+AGG | 0.638621 | 1_1:-7348792 | Msa0004870:CDS |
| CAAGATGACGATGATTGGCG+TGG | 0.680070 | 1_1:-7348765 | Msa0004870:CDS |
| GGGCGGAAACGGGAACAGTG+AGG | 0.685551 | 1_1:-7349229 | Msa0004870:CDS |
| AGATATGGGTGATAATCAGG+AGG | 0.694136 | 1_1:-7348811 | Msa0004870:CDS |
| AAAGGCAAGATAGTGGTGGG+GGG | 0.727123 | 1_1:-7349107 | Msa0004870:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATATCAACAATTGAAAATT+CGG | + | chr1_1:7348992-7349011 | None:intergenic | 15.0% |
| !!! | TTTTTGTAAACAGATTCATA+TGG | - | chr1_1:7348943-7348962 | Msa0004870:CDS | 20.0% |
| !! | TGATAAGCTATTCATTTTGT+GGG | - | chr1_1:7348736-7348755 | Msa0004870:CDS | 25.0% |
| ! | TAAACAGATTCATATGGTTA+GGG | - | chr1_1:7348949-7348968 | Msa0004870:CDS | 25.0% |
| ! | GTGATAAGCTATTCATTTTG+TGG | - | chr1_1:7348735-7348754 | Msa0004870:CDS | 30.0% |
| !! | GATTGATTAGTTTCATTGCT+TGG | - | chr1_1:7348855-7348874 | Msa0004870:CDS | 30.0% |
| GTAAACAGATTCATATGGTT+AGG | - | chr1_1:7348948-7348967 | Msa0004870:CDS | 30.0% | |
| ATATGGTTAGGGATATAGAT+GGG | - | chr1_1:7348960-7348979 | Msa0004870:CDS | 30.0% | |
| ACACAACAAATCTAGCAAAT+TGG | - | chr1_1:7349053-7349072 | Msa0004870:CDS | 30.0% | |
| TAAGGTTGACAAGATTAATG+AGG | - | chr1_1:7349210-7349229 | Msa0004870:CDS | 30.0% | |
| ! | CTACTACCTTTTAAGCCATT+TGG | - | chr1_1:7348790-7348809 | Msa0004870:CDS | 35.0% |
| ! | TTTTAAGCCATTTGGCATCT+GGG | - | chr1_1:7348798-7348817 | Msa0004870:CDS | 35.0% |
| TAGTTTCATTGCTTGGAAGA+TGG | - | chr1_1:7348862-7348881 | Msa0004870:CDS | 35.0% | |
| AGTTTCATTGCTTGGAAGAT+GGG | - | chr1_1:7348863-7348882 | Msa0004870:CDS | 35.0% | |
| CTGAAGAAAATCACATTGAG+AGG | - | chr1_1:7348900-7348919 | Msa0004870:CDS | 35.0% | |
| CATATGGTTAGGGATATAGA+TGG | - | chr1_1:7348959-7348978 | Msa0004870:CDS | 35.0% | |
| ! | AATTTTGTTGCCAAATCCCT+TGG | - | chr1_1:7349024-7349043 | Msa0004870:CDS | 35.0% |
| ! | ATTTTGTTGCCAAATCCCTT+GGG | - | chr1_1:7349025-7349044 | Msa0004870:CDS | 35.0% |
| TGAAGATATGGGTGATAATC+AGG | - | chr1_1:7349126-7349145 | Msa0004870:CDS | 35.0% | |
| TTGCTACAAGCATTTGTCTT+TGG | + | chr1_1:7349246-7349265 | None:intergenic | 35.0% | |
| !! | GCTATTCATTTTGTGGGCTA+TGG | - | chr1_1:7348742-7348761 | Msa0004870:CDS | 40.0% |
| AACAAATGTGCTGTAAACGC+TGG | - | chr1_1:7348767-7348786 | Msa0004870:CDS | 40.0% | |
| CAGATGCCAAATGGCTTAAA+AGG | + | chr1_1:7348799-7348818 | None:intergenic | 40.0% | |
| ! | CTTTTAAGCCATTTGGCATC+TGG | - | chr1_1:7348797-7348816 | Msa0004870:CDS | 40.0% |
| TTTAAGCCATTTGGCATCTG+GGG | - | chr1_1:7348799-7348818 | Msa0004870:CDS | 40.0% | |
| ! | TTTGTTGTGTCAGTTCCCAA+GGG | + | chr1_1:7349043-7349062 | None:intergenic | 40.0% |
| !! | ATTTGTTGTGTCAGTTCCCA+AGG | + | chr1_1:7349044-7349063 | None:intergenic | 40.0% |
| AGATATGGGTGATAATCAGG+AGG | - | chr1_1:7349129-7349148 | Msa0004870:CDS | 40.0% | |
| TCATCTTGCATTCCTCCTAT+AGG | + | chr1_1:7349163-7349182 | None:intergenic | 40.0% | |
| GAATGCAAGATGACGATGAT+TGG | - | chr1_1:7349170-7349189 | Msa0004870:CDS | 40.0% | |
| TCAACCCAAAGGCAAGATAG+TGG | - | chr1_1:7348826-7348845 | Msa0004870:CDS | 45.0% | |
| CCTGCGCAAGATGAAGATAT+GGG | - | chr1_1:7349115-7349134 | Msa0004870:CDS | 45.0% | |
| CAGGAGGAACAACAACCTAT+AGG | - | chr1_1:7349145-7349164 | Msa0004870:CDS | 45.0% | |
| GAGGAACAACAACCTATAGG+AGG | - | chr1_1:7349148-7349167 | Msa0004870:CDS | 45.0% | |
| ! | TGTCCTGCACCATTTTCGGT+AGG | - | chr1_1:7348690-7348709 | Msa0004870:CDS | 50.0% |
| ! | GTCCTGCACCATTTTCGGTA+GGG | - | chr1_1:7348691-7348710 | Msa0004870:CDS | 50.0% |
| ! | CATTTTCGGTAGGGCGGAAA+CGG | - | chr1_1:7348700-7348719 | Msa0004870:CDS | 50.0% |
| ! | ATTTTCGGTAGGGCGGAAAC+GGG | - | chr1_1:7348701-7348720 | Msa0004870:CDS | 50.0% |
| ! | ACCCAAAGGCAAGATAGTGG+TGG | - | chr1_1:7348829-7348848 | Msa0004870:CDS | 50.0% |
| CCCACCACTATCTTGCCTTT+GGG | + | chr1_1:7348833-7348852 | None:intergenic | 50.0% | |
| ! | CCCAAAGGCAAGATAGTGGT+GGG | - | chr1_1:7348830-7348849 | Msa0004870:CDS | 50.0% |
| ! | CCAAAGGCAAGATAGTGGTG+GGG | - | chr1_1:7348831-7348850 | Msa0004870:CDS | 50.0% |
| ! | CAAAGGCAAGATAGTGGTGG+GGG | - | chr1_1:7348832-7348851 | Msa0004870:CDS | 50.0% |
| ! | AAAGGCAAGATAGTGGTGGG+GGG | - | chr1_1:7348833-7348852 | Msa0004870:CDS | 50.0% |
| CATTGCTTGGAAGATGGGAG+CGG | - | chr1_1:7348868-7348887 | Msa0004870:CDS | 50.0% | |
| GTGTCAGTTCCCAAGGGATT+TGG | + | chr1_1:7349037-7349056 | None:intergenic | 50.0% | |
| GCCTGCGCAAGATGAAGATA+TGG | - | chr1_1:7349114-7349133 | Msa0004870:CDS | 50.0% | |
| CCCATATCTTCATCTTGCGC+AGG | + | chr1_1:7349118-7349137 | None:intergenic | 50.0% | |
| CAAGATGACGATGATTGGCG+TGG | - | chr1_1:7349175-7349194 | Msa0004870:CDS | 50.0% | |
| ! | CTGCACCATTTTCGGTAGGG+CGG | - | chr1_1:7348694-7348713 | Msa0004870:CDS | 55.0% |
| CGTTTCCGCCCTACCGAAAA+TGG | + | chr1_1:7348702-7348721 | None:intergenic | 55.0% | |
| AAGCCATTTGGCATCTGGGG+CGG | - | chr1_1:7348802-7348821 | Msa0004870:CDS | 55.0% | |
| CCCCACCACTATCTTGCCTT+TGG | + | chr1_1:7348834-7348853 | None:intergenic | 55.0% | |
| GGCTGCTCTTGAGGTACATG+TGG | + | chr1_1:7349097-7349116 | None:intergenic | 55.0% | |
| CGATGATTGGCGTGGAACGT+TGG | - | chr1_1:7349183-7349202 | Msa0004870:CDS | 55.0% | |
| TGATTGGCGTGGAACGTTGG+AGG | - | chr1_1:7349186-7349205 | Msa0004870:CDS | 55.0% | |
| CGCCCTACCGAAAATGGTGC+AGG | + | chr1_1:7348696-7348715 | None:intergenic | 60.0% | |
| TCTTGCGCAGGCTGCTCTTG+AGG | + | chr1_1:7349106-7349125 | None:intergenic | 60.0% | |
| GCGTGGAACGTTGGAGGCTA+AGG | - | chr1_1:7349192-7349211 | Msa0004870:CDS | 60.0% | |
| GGGCGGAAACGGGAACAGTG+AGG | - | chr1_1:7348711-7348730 | Msa0004870:CDS | 65.0% | |
| GAGCCGCCCCAGATGCCAAA+TGG | + | chr1_1:7348808-7348827 | None:intergenic | 65.0% | |
| ! | TCTGGGGCGGCTCAACCCAA+AGG | - | chr1_1:7348815-7348834 | Msa0004870:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_1 | gene | 7348689 | 7349273 | 7348689 | ID=Msa0004870;Name=Msa0004870 |
| chr1_1 | mRNA | 7348689 | 7349273 | 7348689 | ID=Msa0004870-mRNA-1;Parent=Msa0004870;Name=Msa0004870-mRNA-1;_AED=0.45;_eAED=0.66;_QI=0|-1|0|1|-1|1|1|0|194 |
| chr1_1 | exon | 7348689 | 7349273 | 7348689 | ID=Msa0004870-mRNA-1:exon:2594;Parent=Msa0004870-mRNA-1 |
| chr1_1 | CDS | 7348689 | 7349273 | 7348689 | ID=Msa0004870-mRNA-1:cds;Parent=Msa0004870-mRNA-1 |
| Gene Sequence |
| Protein sequence |
