AlfalfaGEDB | Msa0084250

M. sativa cultivar zhongmu-4 / Msa0084250

KEGG: K02906
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
PFAM: PF00297.25

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0084250	XP_024640795.1	97.959	294	6	0	1	294	1	294	0.0	596

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0084250	sp\|Q9LRN8\|RK3B_ARATH	66.975	324	77	4	1	294	1	324	2.26e-146	416

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0084250	A0A396JZB7	97.959	294	6	0	1	294	1	294	0.0	596

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0036330	Msa0084250	0.812444	4.226329e-51	-8.615850e-47
Msa0040220	Msa0084250	0.801168	1.022472e-48	-8.615850e-47
Msa0084250	Msa0130420	0.917187	7.204807e-86	-8.615850e-47
Msa0084250	Msa0176390	0.800144	1.653864e-48	-8.615850e-47
Msa0084250	Msa0475810	0.814182	1.754728e-51	-8.615850e-47
Msa0084250	Msa1078870	0.801426	9.053095e-49	-8.615850e-47
Msa0084250	Msa1426120	0.810236	1.274107e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0084250	MtrunA17_Chr1g0198561	97.959	294	6	0	1	294	1	294	0.0	596
Msa0084250	MtrunA17_Chr6g0450631	92.517	294	22	0	1	294	1	294	0.0	570
Msa0084250	MtrunA17_Chr2g0324401	94.340	106	6	0	189	294	1	106	1.82e-70	213
Msa0084250	MtrunA17_Chr3g0145851	41.063	207	116	6	49	251	58	262	6.02e-43	148

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0084250	AT3G17465.1	66.975	324	77	4	1	294	1	324	2.30e-147	416
Msa0084250	AT2G43030.1	38.835	206	122	4	49	251	57	261	4.58e-39	138

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Find 106 sgRNAs with CRISPR-Local

Find 309 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACCCTAGATGGACAAAA+GGG	0.565069	1_2:+63594344	None:intergenic
AAAAGGGTATAGTAGGGTGT+TGG	0.525900	1_2:+63594360	None:intergenic
AAAATTCAAATAACAGAACA+TGG	0.608055	1_2:+63590324	None:intergenic
AAACCTTTGGTTGCTGATCT+TGG	0.318983	1_2:-63590426	Msa0084250:CDS
AAAGAGATAGTTCCACAAAT+GGG	0.492226	1_2:-63590374	Msa0084250:three_prime_UTR
AAAGGAAGTATAGATGTGTC+AGG	0.528945	1_2:+63590492	None:intergenic
AAATCGCGGCAGTGGTACCA+CGG	0.587214	1_2:+63593215	None:intergenic
AACCTTTGGTTGCTGATCTT+GGG	0.341776	1_2:-63590425	Msa0084250:CDS
AACTAGAACTTATCGAGTGA+AGG	0.580719	1_2:+63590258	None:intergenic
AAGAAGTATGGATTTCATGG+AGG	0.684184	1_2:-63591421	Msa0084250:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTAGGCTTATCTAGTTAAAG+AGG	-	chr1_2:63590445-63590464	Msa0084250:CDS	30.0%
ACTATGTTAAACTCTCAGAT+TGG	-	chr1_2:63590727-63590746	Msa0084250:intron	30.0%
ATCCTTCAAAGAATATGCTA+GGG	+	chr1_2:63590851-63590870	None:intergenic	30.0%
AATCCTTCAAAGAATATGCT+AGG	+	chr1_2:63590852-63590871	None:intergenic	30.0%
AAATTAGTCCCAAACTTTGT+AGG	+	chr1_2:63590949-63590968	None:intergenic	30.0%
AGTGAGTGGTTGAAATATAA+TGG	+	chr1_2:63590983-63591002	None:intergenic	30.0%
TAAAGTACAAAATTCATCGC+TGG	+	chr1_2:63591157-63591176	None:intergenic	30.0%
ACATCTGAACTAAAAAATCG+CGG	+	chr1_2:63591330-63591349	None:intergenic	30.0%
GAATTCCAATTTCTATCCTT+TGG	-	chr1_2:63591466-63591485	Msa0084250:intron	30.0%
AATCCACATGAAACTCATTA+GGG	+	chr1_2:63591592-63591611	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_2	gene	63590118	63594432	63590118	ID=Msa0084250;Name=Msa0084250
chr1_2	mRNA	63590118	63594432	63590118	ID=Msa0084250-mRNA-1;Parent=Msa0084250;Name=Msa0084250-mRNA-1;_AED=0.16;_eAED=0.16;_QI=160\|1\|1\|1\|0.66\|0.42\|7\|226\|294
chr1_2	exon	63590118	63590199	63590118	ID=Msa0084250-mRNA-1:exon:21357;Parent=Msa0084250-mRNA-1
chr1_2	exon	63590251	63590568	63590251	ID=Msa0084250-mRNA-1:exon:21356;Parent=Msa0084250-mRNA-1
chr1_2	exon	63591102	63591215	63591102	ID=Msa0084250-mRNA-1:exon:21355;Parent=Msa0084250-mRNA-1
chr1_2	exon	63591351	63591452	63591351	ID=Msa0084250-mRNA-1:exon:21354;Parent=Msa0084250-mRNA-1
chr1_2	exon	63592215	63592424	63592215	ID=Msa0084250-mRNA-1:exon:21353;Parent=Msa0084250-mRNA-1
chr1_2	exon	63592999	63593291	63592999	ID=Msa0084250-mRNA-1:exon:21352;Parent=Msa0084250-mRNA-1
chr1_2	exon	63594281	63594432	63594281	ID=Msa0084250-mRNA-1:exon:21351;Parent=Msa0084250-mRNA-1
chr1_2	five_prime_UTR	63594281	63594432	63594281	ID=Msa0084250-mRNA-1:five_prime_utr;Parent=Msa0084250-mRNA-1
chr1_2	five_prime_UTR	63593284	63593291	63593284	ID=Msa0084250-mRNA-1:five_prime_utr;Parent=Msa0084250-mRNA-1
chr1_2	CDS	63592999	63593283	63592999	ID=Msa0084250-mRNA-1:cds;Parent=Msa0084250-mRNA-1
chr1_2	CDS	63592215	63592424	63592215	ID=Msa0084250-mRNA-1:cds;Parent=Msa0084250-mRNA-1
chr1_2	CDS	63591351	63591452	63591351	ID=Msa0084250-mRNA-1:cds;Parent=Msa0084250-mRNA-1
chr1_2	CDS	63591102	63591215	63591102	ID=Msa0084250-mRNA-1:cds;Parent=Msa0084250-mRNA-1
chr1_2	CDS	63590395	63590568	63590395	ID=Msa0084250-mRNA-1:cds;Parent=Msa0084250-mRNA-1
chr1_2	three_prime_UTR	63590251	63590394	63590251	ID=Msa0084250-mRNA-1:three_prime_utr;Parent=Msa0084250-mRNA-1
chr1_2	three_prime_UTR	63590118	63590199	63590118	ID=Msa0084250-mRNA-1:three_prime_utr;Parent=Msa0084250-mRNA-1

Gene Sequence

>Msa0084250

ATGCTGGCGTTTTCAAGAGGTGTGGTTTCTCGTCTTCGACAATTCCCCGTGGTACCACTGCCGCGATTTTTTAGTTCAGATGTGGAGACTATAAGGACGATTGAAGCAAAACCTAGTGTGATGACACCAAATTCACAAAGAACTGGTGTTATAGCTGTAAAATGTGGAATGACTGCTCATTGGGATAAATGGGGTGCTAGAATTCCAATTTCTATCCTTTGGGTAGATGACAACATTGTCTCTCAGGTCAAAACTCCTGAGAAGGAAGGCTATTGTTCTCTCCAGATTGGTTGTGGACAGAAGAAGGAAAAACACTTGACAAAGCCTGAAGTGGGTCATTTTAGGGCTCAAGGAGTTCCGATGAAGAGGAAGCTTCAAGAGTTTCGAGTGACAGAGGATGCACTTCTTCCTGTTGGTACATCACTTAATGTTCGCCATTTTGTTCCCGGGCAGTATGTTGACATCACAGGAATCACCAAAGGAAAAGGTTTTCAGGGTGTAATGAAGAAGTATGGATTTCATGGAGGTTCAGCATCCCATGGGAATTCGAAAGCACATAGAACCATGGGATCTACAGGAATACTTGGTCCTTGTAAGGTCTTTAAAGGTAGAAAGATGCCAGGGAGAATGGGTGGGAAGCAAAGAACAGTAAAAAATGTATGGGTCTACAAAATTGATCCAGCAAGAAATTTGATGTGGGTGAAAGGCCAGGTCCCAGGAGCTACAGGGAACTTTGTTTTTATAAAAGATGCAGTCTTTGAAAAACCTGACACATCTATACTTCCTTTCCCAACTTACTTTGTGCCGGAAGATGAAGATTCAGATGATATGAAACCTTTGGTTGCTGATCTTGGGGATGTGGATCCATTTATGGTTACTGATTAA

Protein sequence

>Msa0084250

MLAFSRGVVSRLRQFPVVPLPRFFSSDVETIRTIEAKPSVMTPNSQRTGVIAVKCGMTAHWDKWGARIPISILWVDDNIVSQVKTPEKEGYCSLQIGCGQKKEKHLTKPEVGHFRAQGVPMKRKLQEFRVTEDALLPVGTSLNVRHFVPGQYVDITGITKGKGFQGVMKKYGFHGGSASHGNSKAHRTMGSTGILGPCKVFKGRKMPGRMGGKQRTVKNVWVYKIDPARNLMWVKGQVPGATGNFVFIKDAVFEKPDTSILPFPTYFVPEDEDSDDMKPLVADLGDVDPFMVTD*