AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0122290

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0122290 MtrunA17_Chr1g0182051 96.667 120 4 0 4 123 3 122 2.78e-77 224
Msa0122290 MtrunA17_Chr4g0030641 89.431 123 13 0 1 123 1 123 2.06e-73 214
Msa0122290 MtrunA17_Chr1g0212921 91.667 120 10 0 4 123 2 121 5.06e-73 213
Msa0122290 MtrunA17_Chr4g0036351 87.395 119 15 0 5 123 3 121 1.16e-67 199
Msa0122290 MtrunA17_Chr7g0249361 85.366 123 18 0 1 123 46 168 1.52e-67 201
Msa0122290 MtrunA17_Chr1g0206641 88.889 36 4 0 14 49 5 40 4.97e-14 61.6
Msa0122290 MtrunA17_Chr5g0419591 87.879 33 4 0 67 99 2 34 2.72e-13 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0122290 AT5G02610.1 88.525 122 14 0 1 122 1 122 5.75e-71 208
Msa0122290 AT2G39390.1 88.525 122 14 0 1 122 1 122 1.20e-70 207
Msa0122290 AT3G09500.1 88.525 122 14 0 1 122 1 122 1.32e-70 207
Msa0122290 AT3G55170.5 86.066 122 17 0 1 122 1 122 1.11e-68 202
Msa0122290 AT3G55170.2 86.066 122 17 0 1 122 1 122 1.11e-68 202
Msa0122290 AT3G55170.1 86.066 122 17 0 1 122 1 122 1.11e-68 202
Msa0122290 AT5G02610.2 74.483 145 14 1 1 122 1 145 3.72e-65 194
Msa0122290 AT3G55170.4 85.859 99 14 0 1 99 1 99 6.81e-53 162
Msa0122290 AT3G55170.3 85.859 99 14 0 1 99 1 99 6.81e-53 162

Find 34 sgRNAs with CRISPR-Local

Find 274 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATACAAGTTAATGAATTA+TGG 0.205495 1_3:-48818220 Msa0122290:three_prime_UTR
AGTTCAATATTTCAAATAAC+TGG 0.271293 1_3:+48818473 None:intergenic
TGCATTTGAAGTGAGAATTA+AGG 0.291537 1_3:-48820270 Msa0122290:intron
CCATAATTCATTAACTTGTA+TGG 0.299454 1_3:+48818220 None:intergenic
CTGATAGCACGGGTCTTCTT+AGG 0.307833 1_3:+48819921 None:intergenic
CTGGTTCAGCAAATCAGCTT+TGG 0.322482 1_3:+48820226 None:intergenic
TTGATTGCATACTTTCTTAG+TGG 0.358328 1_3:+48818529 None:intergenic
GCTTACTTAATTTGTTTGTC+TGG 0.376358 1_3:-48818446 Msa0122290:three_prime_UTR
TGGTTCAGCAAATCAGCTTT+GGG 0.386643 1_3:+48820227 None:intergenic
ATAACACATCGTCGTTAAAA+TGG 0.393667 1_3:-48820460 None:intergenic
AGGTGCACCGCCGGTGACTT+TGG 0.448506 1_3:+48820163 None:intergenic
AAAGTATGCAATCAAAGCTT+AGG 0.474423 1_3:-48818521 Msa0122290:exon
CATAATTCATTAACTTGTAT+GGG 0.474657 1_3:+48818221 None:intergenic
ATTAAACCTGGTGTTTGGTA+AGG 0.478429 1_3:+48819892 None:intergenic
CCGCCTTCAGATCCTTCAGC+TGG 0.492136 1_3:+48820207 None:intergenic
CTTCGAGTCGCCAAAGTCAC+CGG 0.499663 1_3:-48820173 Msa0122290:CDS
AGTGTTTCAGTAGTCTTGAA+AGG 0.534281 1_3:+48818253 None:intergenic
GAGCTTGTTAGGTGCACCGC+CGG 0.541755 1_3:+48820154 None:intergenic
GAATTAAGGTGTTCGAGTTG+AGG 0.547821 1_3:-48820256 Msa0122290:CDS
AATTCATTAACTTGTATGGG+AGG 0.562957 1_3:+48818224 None:intergenic
ATGCAATCAAAGCTTAGGCT+AGG 0.565505 1_3:-48818516 Msa0122290:exon
TGATTTGCTGAACCAGCTGA+AGG 0.570604 1_3:-48820219 Msa0122290:CDS
GACAGAGCGAGAGAAGAAGA+AGG 0.577238 1_3:-48818563 Msa0122290:CDS
ATCTATCTTGAGACTTCCGA+AGG 0.580195 1_3:-48818350 Msa0122290:three_prime_UTR
GAACCAGCTGAAGGATCTGA+AGG 0.580944 1_3:-48820210 Msa0122290:CDS
TAAGGCGTCTTCTGATAGCA+CGG 0.582904 1_3:+48819910 None:intergenic
TTCTTAGGACGAAGATCAAG+TGG 0.602701 1_3:+48819936 None:intergenic
ACCCTACAGTTAAAATCGCA+AGG 0.605131 1_3:+48820436 None:intergenic
AAGGCGTCTTCTGATAGCAC+GGG 0.605672 1_3:+48819911 None:intergenic
AAGACGCCTTACCAAACACC+AGG 0.624051 1_3:-48819898 Msa0122290:intron
ACGGCTATGGAGTGATAGTG+AGG 0.672283 1_3:+48820403 None:intergenic
CGAGTCGCCAAAGTCACCGG+CGG 0.675842 1_3:-48820170 Msa0122290:CDS
CCAGCTGAAGGATCTGAAGG+CGG 0.695519 1_3:-48820207 Msa0122290:CDS
GCAGAAAGCTGCGCTCAGAG+AGG 0.696591 1_3:-48819982 Msa0122290:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTATGTATTGTTTACATTT+TGG + chr1_3:48818527-48818546 None:intergenic 15.0%
!! TTAAGTAAAAAAATTAAACC+TGG + chr1_3:48818777-48818796 None:intergenic 15.0%
!!! TTTTACTTAATTTGTTTGTT+TGG - chr1_3:48818787-48818806 Msa0122290:intron 15.0%
!! TGATTAGATAGTTATTATAA+TGG - chr1_3:48818870-48818889 Msa0122290:intron 15.0%
!! TATGTAATTCTTATAGAATT+TGG - chr1_3:48818988-48819007 Msa0122290:intron 15.0%
!!! TGTTTATCTATAAATTTTAC+TGG - chr1_3:48819262-48819281 Msa0122290:intron 15.0%
!!! ATTTTGAATGAACAAATATA+TGG + chr1_3:48819289-48819308 None:intergenic 15.0%
!! ATATATTTGTTCATTCAAAA+TGG - chr1_3:48819288-48819307 Msa0122290:intron 15.0%
!!! TTTTAAACAAAAACATGAAA+TGG + chr1_3:48819756-48819775 None:intergenic 15.0%
!!! ATAATTTTAACATAAACCTT+CGG + chr1_3:48820323-48820342 None:intergenic 15.0%
!!! ATTATGTATTGTTTACATTT+TGG + chr1_3:48818527-48818546 None:intergenic 15.0%
!! TTAAGTAAAAAAATTAAACC+TGG + chr1_3:48818777-48818796 None:intergenic 15.0%
!!! TTTTACTTAATTTGTTTGTT+TGG - chr1_3:48818787-48818806 Msa0122290:intron 15.0%
!! TGATTAGATAGTTATTATAA+TGG - chr1_3:48818870-48818889 Msa0122290:intron 15.0%
!! TATGTAATTCTTATAGAATT+TGG - chr1_3:48818988-48819007 Msa0122290:intron 15.0%
!!! TGTTTATCTATAAATTTTAC+TGG - chr1_3:48819262-48819281 Msa0122290:intron 15.0%
!!! ATTTTGAATGAACAAATATA+TGG + chr1_3:48819289-48819308 None:intergenic 15.0%
!! ATATATTTGTTCATTCAAAA+TGG - chr1_3:48819288-48819307 Msa0122290:intron 15.0%
!!! TTTTAAACAAAAACATGAAA+TGG + chr1_3:48819756-48819775 None:intergenic 15.0%
!!! ATAATTTTAACATAAACCTT+CGG + chr1_3:48820323-48820342 None:intergenic 15.0%
!!! TTTTTTTTTTGATTCAGGAA+CGG + chr1_3:48818273-48818292 None:intergenic 20.0%
!! TCCTGAATCAAAAAAAAAAA+TGG - chr1_3:48818274-48818293 Msa0122290:three_prime_UTR 20.0%
!! CCTGAATCAAAAAAAAAAAT+GGG - chr1_3:48818275-48818294 Msa0122290:three_prime_UTR 20.0%
!!! AATACATAATCGCTTTTATA+TGG - chr1_3:48818537-48818556 Msa0122290:CDS 20.0%
!! AAAATTGTAATCAAATACGT+TGG - chr1_3:48819547-48819566 Msa0122290:intron 20.0%
!! AAAACTCACAAACAAAATAT+AGG + chr1_3:48819631-48819650 None:intergenic 20.0%
!!! ACTAAGGTTTTCAAATAAAA+AGG + chr1_3:48819962-48819981 None:intergenic 20.0%
!! AGTTCAATATTTCAAATAAC+TGG + chr1_3:48820184-48820203 None:intergenic 20.0%
!! AAATGTAACTAGACAAAAAT+TGG + chr1_3:48820252-48820271 None:intergenic 20.0%
!!! CATAATTCATTAACTTGTAT+GGG + chr1_3:48820436-48820455 None:intergenic 20.0%
!!! TTTTTTTTTTGATTCAGGAA+CGG + chr1_3:48818273-48818292 None:intergenic 20.0%
!! TCCTGAATCAAAAAAAAAAA+TGG - chr1_3:48818274-48818293 Msa0122290:three_prime_UTR 20.0%
!! CCTGAATCAAAAAAAAAAAT+GGG - chr1_3:48818275-48818294 Msa0122290:three_prime_UTR 20.0%
!!! AATACATAATCGCTTTTATA+TGG - chr1_3:48818537-48818556 Msa0122290:CDS 20.0%
!! AAAATTGTAATCAAATACGT+TGG - chr1_3:48819547-48819566 Msa0122290:intron 20.0%
!! AAAACTCACAAACAAAATAT+AGG + chr1_3:48819631-48819650 None:intergenic 20.0%
!!! ACTAAGGTTTTCAAATAAAA+AGG + chr1_3:48819962-48819981 None:intergenic 20.0%
!! AGTTCAATATTTCAAATAAC+TGG + chr1_3:48820184-48820203 None:intergenic 20.0%
!! AAATGTAACTAGACAAAAAT+TGG + chr1_3:48820252-48820271 None:intergenic 20.0%
!!! CATAATTCATTAACTTGTAT+GGG + chr1_3:48820436-48820455 None:intergenic 20.0%
!!! CCCATTTTTTTTTTTGATTC+AGG + chr1_3:48818278-48818297 None:intergenic 25.0%
! AAAAAATTAAACCTGGTGTT+TGG + chr1_3:48818770-48818789 None:intergenic 25.0%
! ATAATTTAGCTAACATTGAG+AGG - chr1_3:48819129-48819148 Msa0122290:intron 25.0%
!! TAAACCAATTAACTGACTTT+GGG + chr1_3:48819240-48819259 None:intergenic 25.0%
! AAATAAAATTGACGAAGCAT+AGG + chr1_3:48819434-48819453 None:intergenic 25.0%
!!! GCTTCGTCAATTTTATTTAT+AGG - chr1_3:48819436-48819455 Msa0122290:intron 25.0%
!! TTTAAAAGTACAATTTCCAG+CGG - chr1_3:48819769-48819788 Msa0122290:intron 25.0%
!!! AAGTTTTACTTTTGCTTGTA+CGG - chr1_3:48820358-48820377 Msa0122290:intron 25.0%
!!! CCATAATTCATTAACTTGTA+TGG + chr1_3:48820437-48820456 None:intergenic 25.0%
! CCATACAAGTTAATGAATTA+TGG - chr1_3:48820434-48820453 Msa0122290:five_prime_UTR 25.0%
!!! CCCATTTTTTTTTTTGATTC+AGG + chr1_3:48818278-48818297 None:intergenic 25.0%
! AAAAAATTAAACCTGGTGTT+TGG + chr1_3:48818770-48818789 None:intergenic 25.0%
! ATAATTTAGCTAACATTGAG+AGG - chr1_3:48819129-48819148 Msa0122290:intron 25.0%
!! TAAACCAATTAACTGACTTT+GGG + chr1_3:48819240-48819259 None:intergenic 25.0%
! AAATAAAATTGACGAAGCAT+AGG + chr1_3:48819434-48819453 None:intergenic 25.0%
!!! GCTTCGTCAATTTTATTTAT+AGG - chr1_3:48819436-48819455 Msa0122290:intron 25.0%
!! TTTAAAAGTACAATTTCCAG+CGG - chr1_3:48819769-48819788 Msa0122290:intron 25.0%
!!! AAGTTTTACTTTTGCTTGTA+CGG - chr1_3:48820358-48820377 Msa0122290:intron 25.0%
!!! CCATAATTCATTAACTTGTA+TGG + chr1_3:48820437-48820456 None:intergenic 25.0%
! CCATACAAGTTAATGAATTA+TGG - chr1_3:48820434-48820453 Msa0122290:five_prime_UTR 25.0%
AATTCTCACTTCAAATGCAA+TGG + chr1_3:48818383-48818402 None:intergenic 30.0%
! TGCATTTGAAGTGAGAATTA+AGG - chr1_3:48818384-48818403 Msa0122290:three_prime_UTR 30.0%
TTATATGGAAGCTGCTTATA+TGG - chr1_3:48818552-48818571 Msa0122290:CDS 30.0%
!!! TTTTTTACAGCAAAGTTGTG+AGG - chr1_3:48818611-48818630 Msa0122290:intron 30.0%
!! TTGCTTTTCTACAACACTAT+TGG + chr1_3:48818850-48818869 None:intergenic 30.0%
!! TTGTAATTTGCTTCTGTATG+TGG - chr1_3:48818946-48818965 Msa0122290:intron 30.0%
! TAGCTATAATTTTCCGATGT+GGG + chr1_3:48819044-48819063 None:intergenic 30.0%
! ATAGCTATAATTTTCCGATG+TGG + chr1_3:48819045-48819064 None:intergenic 30.0%
TCATAGAGATATAACAAGTG+TGG - chr1_3:48819064-48819083 Msa0122290:intron 30.0%
GTTAGCTAAATTATCGATGT+GGG + chr1_3:48819123-48819142 None:intergenic 30.0%
TGTTAGCTAAATTATCGATG+TGG + chr1_3:48819124-48819143 None:intergenic 30.0%
! AAACCAATTAACTGACTTTG+GGG + chr1_3:48819239-48819258 None:intergenic 30.0%
! CTAAACCAATTAACTGACTT+TGG + chr1_3:48819241-48819260 None:intergenic 30.0%
TGCAAATTCTTGAAGAATTC+TGG - chr1_3:48819331-48819350 Msa0122290:intron 30.0%
TATTCTACATTGTAGCAGTT+AGG - chr1_3:48819485-48819504 Msa0122290:intron 30.0%
ATTCTACATTGTAGCAGTTA+GGG - chr1_3:48819486-48819505 Msa0122290:intron 30.0%
AGGGATTTAAACATTGGTAA+TGG - chr1_3:48819505-48819524 Msa0122290:intron 30.0%
! TTCGTATCAGTATCCTATTT+TGG - chr1_3:48819665-48819684 Msa0122290:intron 30.0%
! TCGTATCAGTATCCTATTTT+GGG - chr1_3:48819666-48819685 Msa0122290:intron 30.0%
AAATATATGCACGATTACTG+TGG - chr1_3:48819721-48819740 Msa0122290:intron 30.0%
! TTAAAAGTACAATTTCCAGC+GGG - chr1_3:48819770-48819789 Msa0122290:intron 30.0%
TATTATACACTGTAGCAGTT+AGG - chr1_3:48819795-48819814 Msa0122290:intron 30.0%
ATTATACACTGTAGCAGTTA+GGG - chr1_3:48819796-48819815 Msa0122290:intron 30.0%
TTACAAGGGAAACATCAAAT+GGG + chr1_3:48819993-48820012 None:intergenic 30.0%
TTTACAAGGGAAACATCAAA+TGG + chr1_3:48819994-48820013 None:intergenic 30.0%
GTGTTCAAGTCAATTTACAA+GGG + chr1_3:48820007-48820026 None:intergenic 30.0%
TTGATTGCATACTTTCTTAG+TGG + chr1_3:48820128-48820147 None:intergenic 30.0%
AAAGTATGCAATCAAAGCTT+AGG - chr1_3:48820133-48820152 Msa0122290:intron 30.0%
!! GCTTACTTAATTTGTTTGTC+TGG - chr1_3:48820208-48820227 Msa0122290:CDS 30.0%
AGACAAAAATTGGAATGCAT+AGG + chr1_3:48820242-48820261 None:intergenic 30.0%
!! AATTCATTAACTTGTATGGG+AGG + chr1_3:48820433-48820452 None:intergenic 30.0%
AATTCTCACTTCAAATGCAA+TGG + chr1_3:48818383-48818402 None:intergenic 30.0%
! TGCATTTGAAGTGAGAATTA+AGG - chr1_3:48818384-48818403 Msa0122290:three_prime_UTR 30.0%
TTATATGGAAGCTGCTTATA+TGG - chr1_3:48818552-48818571 Msa0122290:CDS 30.0%
!!! TTTTTTACAGCAAAGTTGTG+AGG - chr1_3:48818611-48818630 Msa0122290:intron 30.0%
!! TTGCTTTTCTACAACACTAT+TGG + chr1_3:48818850-48818869 None:intergenic 30.0%
!! TTGTAATTTGCTTCTGTATG+TGG - chr1_3:48818946-48818965 Msa0122290:intron 30.0%
! TAGCTATAATTTTCCGATGT+GGG + chr1_3:48819044-48819063 None:intergenic 30.0%
! ATAGCTATAATTTTCCGATG+TGG + chr1_3:48819045-48819064 None:intergenic 30.0%
TCATAGAGATATAACAAGTG+TGG - chr1_3:48819064-48819083 Msa0122290:intron 30.0%
GTTAGCTAAATTATCGATGT+GGG + chr1_3:48819123-48819142 None:intergenic 30.0%
TGTTAGCTAAATTATCGATG+TGG + chr1_3:48819124-48819143 None:intergenic 30.0%
! AAACCAATTAACTGACTTTG+GGG + chr1_3:48819239-48819258 None:intergenic 30.0%
! CTAAACCAATTAACTGACTT+TGG + chr1_3:48819241-48819260 None:intergenic 30.0%
TGCAAATTCTTGAAGAATTC+TGG - chr1_3:48819331-48819350 Msa0122290:intron 30.0%
TATTCTACATTGTAGCAGTT+AGG - chr1_3:48819485-48819504 Msa0122290:intron 30.0%
ATTCTACATTGTAGCAGTTA+GGG - chr1_3:48819486-48819505 Msa0122290:intron 30.0%
AGGGATTTAAACATTGGTAA+TGG - chr1_3:48819505-48819524 Msa0122290:intron 30.0%
! TTCGTATCAGTATCCTATTT+TGG - chr1_3:48819665-48819684 Msa0122290:intron 30.0%
! TCGTATCAGTATCCTATTTT+GGG - chr1_3:48819666-48819685 Msa0122290:intron 30.0%
AAATATATGCACGATTACTG+TGG - chr1_3:48819721-48819740 Msa0122290:intron 30.0%
! TTAAAAGTACAATTTCCAGC+GGG - chr1_3:48819770-48819789 Msa0122290:intron 30.0%
TATTATACACTGTAGCAGTT+AGG - chr1_3:48819795-48819814 Msa0122290:intron 30.0%
ATTATACACTGTAGCAGTTA+GGG - chr1_3:48819796-48819815 Msa0122290:intron 30.0%
TTACAAGGGAAACATCAAAT+GGG + chr1_3:48819993-48820012 None:intergenic 30.0%
TTTACAAGGGAAACATCAAA+TGG + chr1_3:48819994-48820013 None:intergenic 30.0%
GTGTTCAAGTCAATTTACAA+GGG + chr1_3:48820007-48820026 None:intergenic 30.0%
TTGATTGCATACTTTCTTAG+TGG + chr1_3:48820128-48820147 None:intergenic 30.0%
AAAGTATGCAATCAAAGCTT+AGG - chr1_3:48820133-48820152 Msa0122290:intron 30.0%
!! GCTTACTTAATTTGTTTGTC+TGG - chr1_3:48820208-48820227 Msa0122290:CDS 30.0%
AGACAAAAATTGGAATGCAT+AGG + chr1_3:48820242-48820261 None:intergenic 30.0%
!! AATTCATTAACTTGTATGGG+AGG + chr1_3:48820433-48820452 None:intergenic 30.0%
!!! TCCTTGCGATTTTAACTGTA+GGG - chr1_3:48818217-48818236 Msa0122290:three_prime_UTR 35.0%
!! TACATTTTGGAGAGCTTGTT+AGG + chr1_3:48818514-48818533 None:intergenic 35.0%
!! TGGTAATCGCGTTTTGTTAA+TGG - chr1_3:48818572-48818591 Msa0122290:CDS 35.0%
ATTAAACCTGGTGTTTGGTA+AGG + chr1_3:48818765-48818784 None:intergenic 35.0%
TGTTTGTTTGGTTGTGTTTC+TGG - chr1_3:48818799-48818818 Msa0122290:intron 35.0%
ATTTGCTTCTGTATGTGGTA+TGG - chr1_3:48818951-48818970 Msa0122290:intron 35.0%
TTCTGTATGTGGTATGGATT+CGG - chr1_3:48818957-48818976 Msa0122290:intron 35.0%
AGCTATCCAATGTGTATCAA+AGG + chr1_3:48819189-48819208 None:intergenic 35.0%
! GTTCATTCAAAATGGTTTGC+TGG - chr1_3:48819296-48819315 Msa0122290:intron 35.0%
GCAGTTAGGGATTTAAACAT+TGG - chr1_3:48819499-48819518 Msa0122290:intron 35.0%
GTAATCAAATACGTTGGATG+TGG - chr1_3:48819553-48819572 Msa0122290:intron 35.0%
TGTTGTAGTCATATTGCAGT+CGG - chr1_3:48819579-48819598 Msa0122290:intron 35.0%
!!! AGGGTTTTAGATAGAGGTAA+TGG - chr1_3:48819815-48819834 Msa0122290:intron 35.0%
! ATAGAGGTAATGGTTGTGTA+GGG - chr1_3:48819825-48819844 Msa0122290:intron 35.0%
! AACAATGGGACTGCAATTTT+AGG + chr1_3:48819887-48819906 None:intergenic 35.0%
GTTATCTCTCCAACAACAAT+GGG + chr1_3:48819901-48819920 None:intergenic 35.0%
TAACGAAAACTCTGATGTTG+TGG - chr1_3:48819917-48819936 Msa0122290:CDS 35.0%
CGTGTTCAAGTCAATTTACA+AGG + chr1_3:48820008-48820027 None:intergenic 35.0%
AGATGATGCATAAATACTGC+CGG + chr1_3:48820037-48820056 None:intergenic 35.0%
! AGTGTTTCAGTAGTCTTGAA+AGG + chr1_3:48820404-48820423 None:intergenic 35.0%
!!! TCCTTGCGATTTTAACTGTA+GGG - chr1_3:48818217-48818236 Msa0122290:three_prime_UTR 35.0%
!! TACATTTTGGAGAGCTTGTT+AGG + chr1_3:48818514-48818533 None:intergenic 35.0%
!! TGGTAATCGCGTTTTGTTAA+TGG - chr1_3:48818572-48818591 Msa0122290:CDS 35.0%
ATTAAACCTGGTGTTTGGTA+AGG + chr1_3:48818765-48818784 None:intergenic 35.0%
TGTTTGTTTGGTTGTGTTTC+TGG - chr1_3:48818799-48818818 Msa0122290:intron 35.0%
ATTTGCTTCTGTATGTGGTA+TGG - chr1_3:48818951-48818970 Msa0122290:intron 35.0%
TTCTGTATGTGGTATGGATT+CGG - chr1_3:48818957-48818976 Msa0122290:intron 35.0%
AGCTATCCAATGTGTATCAA+AGG + chr1_3:48819189-48819208 None:intergenic 35.0%
! GTTCATTCAAAATGGTTTGC+TGG - chr1_3:48819296-48819315 Msa0122290:intron 35.0%
GCAGTTAGGGATTTAAACAT+TGG - chr1_3:48819499-48819518 Msa0122290:intron 35.0%
GTAATCAAATACGTTGGATG+TGG - chr1_3:48819553-48819572 Msa0122290:intron 35.0%
TGTTGTAGTCATATTGCAGT+CGG - chr1_3:48819579-48819598 Msa0122290:intron 35.0%
!!! AGGGTTTTAGATAGAGGTAA+TGG - chr1_3:48819815-48819834 Msa0122290:intron 35.0%
! ATAGAGGTAATGGTTGTGTA+GGG - chr1_3:48819825-48819844 Msa0122290:intron 35.0%
! AACAATGGGACTGCAATTTT+AGG + chr1_3:48819887-48819906 None:intergenic 35.0%
GTTATCTCTCCAACAACAAT+GGG + chr1_3:48819901-48819920 None:intergenic 35.0%
TAACGAAAACTCTGATGTTG+TGG - chr1_3:48819917-48819936 Msa0122290:CDS 35.0%
CGTGTTCAAGTCAATTTACA+AGG + chr1_3:48820008-48820027 None:intergenic 35.0%
AGATGATGCATAAATACTGC+CGG + chr1_3:48820037-48820056 None:intergenic 35.0%
! AGTGTTTCAGTAGTCTTGAA+AGG + chr1_3:48820404-48820423 None:intergenic 35.0%
!!! GTCCTTGCGATTTTAACTGT+AGG - chr1_3:48818216-48818235 Msa0122290:three_prime_UTR 40.0%
ACCCTACAGTTAAAATCGCA+AGG + chr1_3:48818221-48818240 None:intergenic 40.0%
!! TTTTGATTCAGGAACGGCTA+TGG + chr1_3:48818267-48818286 None:intergenic 40.0%
!! GAATTAAGGTGTTCGAGTTG+AGG - chr1_3:48818398-48818417 Msa0122290:three_prime_UTR 40.0%
TGGTTCAGCAAATCAGCTTT+GGG + chr1_3:48818430-48818449 None:intergenic 40.0%
TTCTTAGGACGAAGATCAAG+TGG + chr1_3:48818721-48818740 None:intergenic 40.0%
TCTGTATGTGGTATGGATTC+GGG - chr1_3:48818958-48818977 Msa0122290:intron 40.0%
TACAAATTAGCAGCCCACAT+CGG - chr1_3:48819028-48819047 Msa0122290:intron 40.0%
ATCCAATGTGTATCAAAGGC+TGG + chr1_3:48819185-48819204 None:intergenic 40.0%
!! CACATTGGATAGCTTTGAAC+TGG - chr1_3:48819195-48819214 Msa0122290:intron 40.0%
!! TTGGATAGCTTTGAACTGGT+TGG - chr1_3:48819199-48819218 Msa0122290:intron 40.0%
ACACCCCAAAGTCAGTTAAT+TGG - chr1_3:48819233-48819252 Msa0122290:intron 40.0%
ATTCGCAGAAGTAACGATAC+CGG - chr1_3:48819361-48819380 Msa0122290:intron 40.0%
ATGGTTGTGTCGCAATTATG+TGG - chr1_3:48819524-48819543 Msa0122290:intron 40.0%
CAAAATATAGGCTGCAGCAT+CGG + chr1_3:48819619-48819638 None:intergenic 40.0%
CACATGCTGAAACCCAAAAT+AGG + chr1_3:48819681-48819700 None:intergenic 40.0%
ACAGTGTATAATATACCCGC+TGG + chr1_3:48819788-48819807 None:intergenic 40.0%
!!! GCAGTTAGGGTTTTAGATAG+AGG - chr1_3:48819809-48819828 Msa0122290:intron 40.0%
! GATAGAGGTAATGGTTGTGT+AGG - chr1_3:48819824-48819843 Msa0122290:intron 40.0%
AATTGCAGTCCCATTGTTGT+TGG - chr1_3:48819889-48819908 Msa0122290:intron 40.0%
CGTTATCTCTCCAACAACAA+TGG + chr1_3:48819902-48819921 None:intergenic 40.0%
CAAATGGGTGAATGCTACTA+AGG + chr1_3:48819978-48819997 None:intergenic 40.0%
GATGATGCATAAATACTGCC+GGG + chr1_3:48820036-48820055 None:intergenic 40.0%
! CATCTTACCAGCCTTTTGTT+TGG - chr1_3:48820052-48820071 Msa0122290:intron 40.0%
ATGCAATCAAAGCTTAGGCT+AGG - chr1_3:48820138-48820157 Msa0122290:intron 40.0%
ATCTATCTTGAGACTTCCGA+AGG - chr1_3:48820304-48820323 Msa0122290:intron 40.0%
!!! GTCCTTGCGATTTTAACTGT+AGG - chr1_3:48818216-48818235 Msa0122290:three_prime_UTR 40.0%
ACCCTACAGTTAAAATCGCA+AGG + chr1_3:48818221-48818240 None:intergenic 40.0%
!! TTTTGATTCAGGAACGGCTA+TGG + chr1_3:48818267-48818286 None:intergenic 40.0%
!! GAATTAAGGTGTTCGAGTTG+AGG - chr1_3:48818398-48818417 Msa0122290:three_prime_UTR 40.0%
TGGTTCAGCAAATCAGCTTT+GGG + chr1_3:48818430-48818449 None:intergenic 40.0%
TTCTTAGGACGAAGATCAAG+TGG + chr1_3:48818721-48818740 None:intergenic 40.0%
TCTGTATGTGGTATGGATTC+GGG - chr1_3:48818958-48818977 Msa0122290:intron 40.0%
TACAAATTAGCAGCCCACAT+CGG - chr1_3:48819028-48819047 Msa0122290:intron 40.0%
ATCCAATGTGTATCAAAGGC+TGG + chr1_3:48819185-48819204 None:intergenic 40.0%
!! CACATTGGATAGCTTTGAAC+TGG - chr1_3:48819195-48819214 Msa0122290:intron 40.0%
!! TTGGATAGCTTTGAACTGGT+TGG - chr1_3:48819199-48819218 Msa0122290:intron 40.0%
ACACCCCAAAGTCAGTTAAT+TGG - chr1_3:48819233-48819252 Msa0122290:intron 40.0%
ATTCGCAGAAGTAACGATAC+CGG - chr1_3:48819361-48819380 Msa0122290:intron 40.0%
ATGGTTGTGTCGCAATTATG+TGG - chr1_3:48819524-48819543 Msa0122290:intron 40.0%
CAAAATATAGGCTGCAGCAT+CGG + chr1_3:48819619-48819638 None:intergenic 40.0%
CACATGCTGAAACCCAAAAT+AGG + chr1_3:48819681-48819700 None:intergenic 40.0%
ACAGTGTATAATATACCCGC+TGG + chr1_3:48819788-48819807 None:intergenic 40.0%
!!! GCAGTTAGGGTTTTAGATAG+AGG - chr1_3:48819809-48819828 Msa0122290:intron 40.0%
! GATAGAGGTAATGGTTGTGT+AGG - chr1_3:48819824-48819843 Msa0122290:intron 40.0%
AATTGCAGTCCCATTGTTGT+TGG - chr1_3:48819889-48819908 Msa0122290:intron 40.0%
CGTTATCTCTCCAACAACAA+TGG + chr1_3:48819902-48819921 None:intergenic 40.0%
CAAATGGGTGAATGCTACTA+AGG + chr1_3:48819978-48819997 None:intergenic 40.0%
GATGATGCATAAATACTGCC+GGG + chr1_3:48820036-48820055 None:intergenic 40.0%
! CATCTTACCAGCCTTTTGTT+TGG - chr1_3:48820052-48820071 Msa0122290:intron 40.0%
ATGCAATCAAAGCTTAGGCT+AGG - chr1_3:48820138-48820157 Msa0122290:intron 40.0%
ATCTATCTTGAGACTTCCGA+AGG - chr1_3:48820304-48820323 Msa0122290:intron 40.0%
CTGGTTCAGCAAATCAGCTT+TGG + chr1_3:48818431-48818450 None:intergenic 45.0%
!! TGATTTGCTGAACCAGCTGA+AGG - chr1_3:48818435-48818454 Msa0122290:three_prime_UTR 45.0%
! TAAGGCGTCTTCTGATAGCA+CGG + chr1_3:48818747-48818766 None:intergenic 45.0%
GACCAGCCTTTGATACACAT+TGG - chr1_3:48819180-48819199 Msa0122290:intron 45.0%
GCAGATTGGTACTCATGTAC+CGG + chr1_3:48819383-48819402 None:intergenic 45.0%
GGTACATGAGTACCAATCTG+CGG - chr1_3:48819382-48819401 Msa0122290:intron 45.0%
TGACTTGAACACGTGTTTCC+CGG - chr1_3:48820015-48820034 Msa0122290:CDS 45.0%
CTGGTTCAGCAAATCAGCTT+TGG + chr1_3:48818431-48818450 None:intergenic 45.0%
!! TGATTTGCTGAACCAGCTGA+AGG - chr1_3:48818435-48818454 Msa0122290:three_prime_UTR 45.0%
! TAAGGCGTCTTCTGATAGCA+CGG + chr1_3:48818747-48818766 None:intergenic 45.0%
GACCAGCCTTTGATACACAT+TGG - chr1_3:48819180-48819199 Msa0122290:intron 45.0%
GCAGATTGGTACTCATGTAC+CGG + chr1_3:48819383-48819402 None:intergenic 45.0%
GGTACATGAGTACCAATCTG+CGG - chr1_3:48819382-48819401 Msa0122290:intron 45.0%
TGACTTGAACACGTGTTTCC+CGG - chr1_3:48820015-48820034 Msa0122290:CDS 45.0%
! ACGGCTATGGAGTGATAGTG+AGG + chr1_3:48818254-48818273 None:intergenic 50.0%
GAACCAGCTGAAGGATCTGA+AGG - chr1_3:48818444-48818463 Msa0122290:three_prime_UTR 50.0%
CTGATAGCACGGGTCTTCTT+AGG + chr1_3:48818736-48818755 None:intergenic 50.0%
! AAGGCGTCTTCTGATAGCAC+GGG + chr1_3:48818746-48818765 None:intergenic 50.0%
AAGACGCCTTACCAAACACC+AGG - chr1_3:48818756-48818775 Msa0122290:intron 50.0%
CAGAATTCGCAGGACCACTT+GGG + chr1_3:48819409-48819428 None:intergenic 50.0%
ACAGAATTCGCAGGACCACT+TGG + chr1_3:48819410-48819429 None:intergenic 50.0%
AGCATAGGCACAGAATTCGC+AGG + chr1_3:48819419-48819438 None:intergenic 50.0%
TGTGGCGCAGATAGTTGTCA+TGG - chr1_3:48819935-48819954 Msa0122290:CDS 50.0%
!!! ACCAGCCTTTTGTTTGGTGC+AGG - chr1_3:48820058-48820077 Msa0122290:intron 50.0%
CGATGCCTGCACCAAACAAA+AGG + chr1_3:48820066-48820085 None:intergenic 50.0%
GACAGAGCGAGAGAAGAAGA+AGG - chr1_3:48820091-48820110 Msa0122290:intron 50.0%
! ACGGCTATGGAGTGATAGTG+AGG + chr1_3:48818254-48818273 None:intergenic 50.0%
GAACCAGCTGAAGGATCTGA+AGG - chr1_3:48818444-48818463 Msa0122290:three_prime_UTR 50.0%
CTGATAGCACGGGTCTTCTT+AGG + chr1_3:48818736-48818755 None:intergenic 50.0%
! AAGGCGTCTTCTGATAGCAC+GGG + chr1_3:48818746-48818765 None:intergenic 50.0%
AAGACGCCTTACCAAACACC+AGG - chr1_3:48818756-48818775 Msa0122290:intron 50.0%
CAGAATTCGCAGGACCACTT+GGG + chr1_3:48819409-48819428 None:intergenic 50.0%
ACAGAATTCGCAGGACCACT+TGG + chr1_3:48819410-48819429 None:intergenic 50.0%
AGCATAGGCACAGAATTCGC+AGG + chr1_3:48819419-48819438 None:intergenic 50.0%
TGTGGCGCAGATAGTTGTCA+TGG - chr1_3:48819935-48819954 Msa0122290:CDS 50.0%
!!! ACCAGCCTTTTGTTTGGTGC+AGG - chr1_3:48820058-48820077 Msa0122290:intron 50.0%
CGATGCCTGCACCAAACAAA+AGG + chr1_3:48820066-48820085 None:intergenic 50.0%
GACAGAGCGAGAGAAGAAGA+AGG - chr1_3:48820091-48820110 Msa0122290:intron 50.0%
CCAGCTGAAGGATCTGAAGG+CGG - chr1_3:48818447-48818466 Msa0122290:three_prime_UTR 55.0%
CTTCGAGTCGCCAAAGTCAC+CGG - chr1_3:48818481-48818500 Msa0122290:three_prime_UTR 55.0%
!! GACTTTGGGGTGTGCATCAC+AGG + chr1_3:48819226-48819245 None:intergenic 55.0%
TACCAATCTGCGGACCCAAG+TGG - chr1_3:48819392-48819411 Msa0122290:intron 55.0%
GCCTGCACCAAACAAAAGGC+TGG + chr1_3:48820062-48820081 None:intergenic 55.0%
CCAGCTGAAGGATCTGAAGG+CGG - chr1_3:48818447-48818466 Msa0122290:three_prime_UTR 55.0%
CTTCGAGTCGCCAAAGTCAC+CGG - chr1_3:48818481-48818500 Msa0122290:three_prime_UTR 55.0%
!! GACTTTGGGGTGTGCATCAC+AGG + chr1_3:48819226-48819245 None:intergenic 55.0%
TACCAATCTGCGGACCCAAG+TGG - chr1_3:48819392-48819411 Msa0122290:intron 55.0%
GCCTGCACCAAACAAAAGGC+TGG + chr1_3:48820062-48820081 None:intergenic 55.0%
CCGCCTTCAGATCCTTCAGC+TGG + chr1_3:48818450-48818469 None:intergenic 60.0%
!! GAGCTTGTTAGGTGCACCGC+CGG + chr1_3:48818503-48818522 None:intergenic 60.0%
GCAGAAAGCTGCGCTCAGAG+AGG - chr1_3:48818672-48818691 Msa0122290:intron 60.0%
GACCACTTGGGTCCGCAGAT+TGG + chr1_3:48819397-48819416 None:intergenic 60.0%
CCGCCTTCAGATCCTTCAGC+TGG + chr1_3:48818450-48818469 None:intergenic 60.0%
!! GAGCTTGTTAGGTGCACCGC+CGG + chr1_3:48818503-48818522 None:intergenic 60.0%
GCAGAAAGCTGCGCTCAGAG+AGG - chr1_3:48818672-48818691 Msa0122290:intron 60.0%
GACCACTTGGGTCCGCAGAT+TGG + chr1_3:48819397-48819416 None:intergenic 60.0%
CGAGTCGCCAAAGTCACCGG+CGG - chr1_3:48818484-48818503 Msa0122290:three_prime_UTR 65.0%
AGGTGCACCGCCGGTGACTT+TGG + chr1_3:48818494-48818513 None:intergenic 65.0%
CGAGTCGCCAAAGTCACCGG+CGG - chr1_3:48818484-48818503 Msa0122290:three_prime_UTR 65.0%
AGGTGCACCGCCGGTGACTT+TGG + chr1_3:48818494-48818513 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 48818208 48820468 48818208 ID=Msa0122290;Name=Msa0122290
chr1_3 mRNA 48818208 48820468 48818208 ID=Msa0122290-mRNA-1;Parent=Msa0122290;Name=Msa0122290-mRNA-1;_AED=0.06;_eAED=0.06;_QI=85|1|1|1|1|1|4|314|123
chr1_3 exon 48818208 48818596 48818208 ID=Msa0122290-mRNA-1:exon:14776;Parent=Msa0122290-mRNA-1
chr1_3 exon 48819899 48820055 48819899 ID=Msa0122290-mRNA-1:exon:14775;Parent=Msa0122290-mRNA-1
chr1_3 exon 48820146 48820281 48820146 ID=Msa0122290-mRNA-1:exon:14774;Parent=Msa0122290-mRNA-1
chr1_3 exon 48820380 48820468 48820380 ID=Msa0122290-mRNA-1:exon:14773;Parent=Msa0122290-mRNA-1
chr1_3 five_prime_UTR 48820384 48820468 48820384 ID=Msa0122290-mRNA-1:five_prime_utr;Parent=Msa0122290-mRNA-1
chr1_3 CDS 48820380 48820383 48820380 ID=Msa0122290-mRNA-1:cds;Parent=Msa0122290-mRNA-1
chr1_3 CDS 48820146 48820281 48820146 ID=Msa0122290-mRNA-1:cds;Parent=Msa0122290-mRNA-1
chr1_3 CDS 48819899 48820055 48819899 ID=Msa0122290-mRNA-1:cds;Parent=Msa0122290-mRNA-1
chr1_3 CDS 48818522 48818596 48818522 ID=Msa0122290-mRNA-1:cds;Parent=Msa0122290-mRNA-1
chr1_3 three_prime_UTR 48818208 48818521 48818208 ID=Msa0122290-mRNA-1:three_prime_utr;Parent=Msa0122290-mRNA-1
Gene Sequence

>Msa0122290

ATGGTGAGAATTAAGGTGTTCGAGTTGAGGAACAAACCCAAAGCTGATTTGCTGAACCAGCTGAAGGATCTGAAGGCGGAGCTCGCTTTGCTTCGAGTCGCCAAAGTCACCGGCGGTGCACCTAACAAGCTCTCCAAAATCAAAGTTGTGAGGCTTTCAATTGCACAAGTGTTGACAGTGATTTCACAGAAGCAGAAAGCTGCGCTCAGAGAGGTTTACAAAAATAAGAAGTATTTACCACTTGATCTTCGTCCTAAGAAGACCCGTGCTATCAGAAGACGCCTTACCAAACACCAGGCATCGTTGAAGACAGAGCGAGAGAAGAAGAAGGAGATATACTTTCCACTAAGAAAGTATGCAATCAAAGCTTAG
Protein sequence

>Msa0122290

MVRIKVFELRNKPKADLLNQLKDLKAELALLRVAKVTGGAPNKLSKIKVVRLSIAQVLTVISQKQKAALREVYKNKKYLPLDLRPKKTRAIRRRLTKHQASLKTEREKKKEIYFPLRKYAIKA*