AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0135850

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0135850 MtrunA17_Chr1g0206811 94.931 217 8 1 1 217 1 214 1.78e-151 419
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0135850 AT1G50020.1 52.830 212 88 5 8 217 8 209 4.14e-70 213

Find 52 sgRNAs with CRISPR-Local

Find 158 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAGAATTTCTGGGAAAAT+TGG 0.118542 1_3:-67612660 None:intergenic
ATTATTCCATGAAGAATTTC+TGG 0.158065 1_3:-67612669 None:intergenic
TAGCTCCTCGGCTAAATTAA+AGG 0.278659 1_3:+67615464 Msa0135850:CDS
TCAACACTTCCAAGTCTTCC+AGG 0.320266 1_3:+67612741 Msa0135850:CDS
GGAACATTCTTCTTGGCATT+TGG 0.330859 1_3:+67614560 Msa0135850:CDS
GAATCAGAGGGAACGGTCTA+TGG 0.337234 1_3:+67613885 Msa0135850:CDS
ACTTGGAGGAACATTCTTCT+TGG 0.347709 1_3:+67614553 Msa0135850:CDS
CTTCTGTGAATGAAGAAGAT+AGG 0.362753 1_3:+67613812 Msa0135850:CDS
GATTACAAGCAAACCAACTA+TGG 0.380799 1_3:+67613775 Msa0135850:CDS
CTTCCGTCGTGGACAAAACT+TGG 0.396329 1_3:-67615370 None:intergenic
TCATCTTCTAAGAGTGCATA+AGG 0.401040 1_3:-67615427 None:intergenic
CTCTTCATCAACTCCATAGT+TGG 0.402993 1_3:-67613788 None:intergenic
GGGAAAATTGGATTGTGAAA+GGG 0.446334 1_3:-67612648 None:intergenic
TTCTGTGAATGAAGAAGATA+GGG 0.460643 1_3:+67613813 Msa0135850:CDS
TGGGAAAATTGGATTGTGAA+AGG 0.466227 1_3:-67612649 None:intergenic
GTGAAAGGGAAAGTTGAGAG+AGG 0.470279 1_3:-67612634 None:intergenic
TTATTCCATGAAGAATTTCT+GGG 0.471291 1_3:-67612668 None:intergenic
ACAACAGACCCTGGAAGACT+TGG 0.496923 1_3:-67612750 None:intergenic
CAACACTTCCAAGTCTTCCA+GGG 0.512123 1_3:+67612742 Msa0135850:CDS
AAACTTCTATTGACACTTGG+AGG 0.515017 1_3:+67614539 Msa0135850:CDS
TAATCCTCAAGCAATGATGT+AGG 0.515998 1_3:-67613757 None:intergenic
AATCCTCAAGCAATGATGTA+GGG 0.519413 1_3:-67613756 None:intergenic
ACTATCACCTCCACAATATG+TGG 0.522313 1_3:+67615402 Msa0135850:CDS
CTCTAGACCCCTCTCAACAT+TGG 0.523903 1_3:-67613863 None:intergenic
TTGAAGGAATTGAAGAGGAG+AGG 0.525995 1_3:+67613727 Msa0135850:CDS
TGAAAGGGAAAGTTGAGAGA+GGG 0.536654 1_3:-67612633 None:intergenic
CATCTTCTAAGAGTGCATAA+GGG 0.537509 1_3:-67615426 None:intergenic
GCATAAGGGTCCACATATTG+TGG 0.543635 1_3:-67615412 None:intergenic
AGAGGGGTCTAGAGAATCAG+AGG 0.544137 1_3:+67613872 Msa0135850:CDS
GTCTAGAGAATCAGAGGGAA+CGG 0.571889 1_3:+67613878 Msa0135850:CDS
AGGGAAAGTTGAGAGAGGGT+TGG 0.572433 1_3:-67612629 None:intergenic
GACCCCTACATCATTGCTTG+AGG 0.575241 1_3:+67613753 Msa0135850:CDS
ATGCACTCTTAGAAGATGAA+AGG 0.586584 1_3:+67615431 Msa0135850:CDS
GCTAAACTTCTATTGACACT+TGG 0.589619 1_3:+67614536 Msa0135850:CDS
AGTGTCAACCAATGTTGAGA+GGG 0.601124 1_3:+67613855 Msa0135850:CDS
GCCACGGTTATGAGGATCAA+CGG 0.602581 1_3:-67614584 None:intergenic
AGAACTACTACAACAGACCC+TGG 0.607538 1_3:-67612759 None:intergenic
CAACTATGGAGTTGATGAAG+AGG 0.608576 1_3:+67613789 Msa0135850:CDS
CAGTGTCAACCAATGTTGAG+AGG 0.610884 1_3:+67613854 Msa0135850:CDS
GGATATCTCAGATAGCTCCT+CGG 0.629394 1_3:+67615452 Msa0135850:CDS
GTGACATCTTGCTTCCGTCG+TGG 0.633182 1_3:-67615381 None:intergenic
GCTGAACAGTGAAGGTCTTG+AGG 0.634680 1_3:+67613909 Msa0135850:CDS
ATCCTCAAGCAATGATGTAG+GGG 0.635911 1_3:-67613755 None:intergenic
GATGCCAAGAATCAAAACAA+TGG 0.639737 1_3:+67612785 Msa0135850:CDS
TCTATGGCGCTGAACAGTGA+AGG 0.641145 1_3:+67613901 Msa0135850:CDS
GTCAATAGAAGTTTAGCTCG+AGG 0.648431 1_3:-67614530 None:intergenic
GAGGGGTCTAGAGAATCAGA+GGG 0.670863 1_3:+67613873 Msa0135850:CDS
TAAGGGTCCACATATTGTGG+AGG 0.678073 1_3:-67615409 None:intergenic
AGAGCTTACGAAGTAAAGAG+CGG 0.697103 1_3:-67614615 None:intergenic
GTGTCAACCAATGTTGAGAG+GGG 0.698061 1_3:+67613856 Msa0135850:CDS
ACCGTTGATCCTCATAACCG+TGG 0.700978 1_3:+67614583 Msa0135850:CDS
TCTGTGAATGAAGAAGATAG+GGG 0.715285 1_3:+67613814 Msa0135850:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CAAAAAACAATTTTTATCAA+CGG - chr1_3:67613130-67613149 None:intergenic 15.0%
!!! TATATTTTGAGAATCAATTT+TGG + chr1_3:67613366-67613385 Msa0135850:intron 15.0%
!!! TTTTACATACTTTCTAAATA+GGG + chr1_3:67614363-67614382 Msa0135850:intron 15.0%
!! TATTTACTACAATACAAATA+AGG - chr1_3:67614393-67614412 None:intergenic 15.0%
!!! AGAATATACATTTTTTCTAA+AGG - chr1_3:67614864-67614883 None:intergenic 15.0%
!! TTTGTGAAAAAAACATTGTT+GGG - chr1_3:67612699-67612718 None:intergenic 20.0%
!!! TTTTGTGAAAAAAACATTGT+TGG - chr1_3:67612700-67612719 None:intergenic 20.0%
!!! TGTTGAAATTTTGAAACTTT+AGG - chr1_3:67612727-67612746 None:intergenic 20.0%
!!! TTTTATGTATTGATGTTGAT+TGG + chr1_3:67612963-67612982 Msa0135850:intron 20.0%
!!! AAATTGTTTTTTGAGTGAAT+TGG + chr1_3:67613137-67613156 Msa0135850:intron 20.0%
!!! TGGTTATGTAAAATTGTTTT+TGG + chr1_3:67613157-67613176 Msa0135850:intron 20.0%
!!! AAAAATTTCATGTTGTGTTT+TGG + chr1_3:67613411-67613430 Msa0135850:intron 20.0%
!! TAAAGTGAAATATTTGAATG+TGG + chr1_3:67613513-67613532 Msa0135850:intron 20.0%
!!! TATATAAAAAATCGCTGATT+TGG + chr1_3:67613963-67613982 Msa0135850:intron 20.0%
!!! GTTTTACATACTTTCTAAAT+AGG + chr1_3:67614362-67614381 Msa0135850:intron 20.0%
!! ATAAATATGTAAGTGATTTC+TGG - chr1_3:67614492-67614511 None:intergenic 20.0%
!! TTACATATTTATCCATTTCA+GGG + chr1_3:67614500-67614519 Msa0135850:intron 20.0%
!! ATAGTACACATTTAATGATA+TGG + chr1_3:67614705-67614724 Msa0135850:intron 20.0%
!! TAATGTTAAACATAAACCAT+AGG - chr1_3:67615097-67615116 None:intergenic 20.0%
! TTATTCCATGAAGAATTTCT+GGG - chr1_3:67612671-67612690 None:intergenic 25.0%
! ATTATTCCATGAAGAATTTC+TGG - chr1_3:67612672-67612691 None:intergenic 25.0%
!!! CTTTAGAGTAGTAATGATTT+TGG + chr1_3:67612822-67612841 Msa0135850:intron 25.0%
! ATCCATCATCATACAAAAAA+AGG - chr1_3:67612910-67612929 None:intergenic 25.0%
! CTTGAATATAAATACTCATG+AGG + chr1_3:67612988-67613007 Msa0135850:intron 25.0%
! TGATGAGTTCATATATTGTT+TGG + chr1_3:67613018-67613037 Msa0135850:intron 25.0%
! TTCAAGTAGAATCAATTCTA+GGG + chr1_3:67613295-67613314 Msa0135850:intron 25.0%
!!! AGAATTGATTTTGTCATGTT+TGG - chr1_3:67613347-67613366 None:intergenic 25.0%
!! ACACAACATGAAATTTTTCT+AGG - chr1_3:67613409-67613428 None:intergenic 25.0%
! TCACTTTATCAAATTATCAC+AGG - chr1_3:67613501-67613520 None:intergenic 25.0%
!!! CTAATACTGTATTGATTTTC+AGG + chr1_3:67613679-67613698 Msa0135850:intron 25.0%
!!! TATTTTTGAAGGAATTGAAG+AGG + chr1_3:67613722-67613741 Msa0135850:CDS 25.0%
! TATTGAAGGAAAGTTAACAA+CGG - chr1_3:67613940-67613959 None:intergenic 25.0%
! CTTACATATTTATCCATTTC+AGG + chr1_3:67614499-67614518 Msa0135850:intron 25.0%
! TGAAGTGAATAACAATTTGA+TGG + chr1_3:67614767-67614786 Msa0135850:intron 25.0%
!!! ATATTCTAGCTTATAACAGT+TGG + chr1_3:67614877-67614896 Msa0135850:intron 25.0%
! CAACGTTCTATATTATATGA+AGG - chr1_3:67615315-67615334 None:intergenic 25.0%
! CATATAATATAGAACGTTGA+TGG + chr1_3:67615316-67615335 Msa0135850:intron 25.0%
! TGAAGAATTTCTGGGAAAAT+TGG - chr1_3:67612663-67612682 None:intergenic 30.0%
! ATTTTCCCAGAAATTCTTCA+TGG + chr1_3:67612663-67612682 Msa0135850:CDS 30.0%
TGAAAAAAACATTGTTGGGT+TGG - chr1_3:67612695-67612714 None:intergenic 30.0%
!!! AGCCTTTTTTTGTATGATGA+TGG + chr1_3:67612905-67612924 Msa0135850:intron 30.0%
GAATATAAATACTCATGAGG+TGG + chr1_3:67612991-67613010 Msa0135850:intron 30.0%
GAGTTCATATATTGTTTGGA+TGG + chr1_3:67613022-67613041 Msa0135850:intron 30.0%
ACACTTATGTCCTTATAGTT+TGG + chr1_3:67613065-67613084 Msa0135850:intron 30.0%
!!! TGTTTACTTGGTGTATGTTT+TGG + chr1_3:67613100-67613119 Msa0135850:intron 30.0%
TGAAGCAAGTGATTTATGTT+CGG + chr1_3:67613192-67613211 Msa0135850:intron 30.0%
CTTCAAGTAGAATCAATTCT+AGG + chr1_3:67613294-67613313 Msa0135850:intron 30.0%
TCAAGTAGAATCAATTCTAG+GGG + chr1_3:67613296-67613315 Msa0135850:intron 30.0%
! TAACTAACTGTACCTTTTGA+TGG + chr1_3:67613646-67613665 Msa0135850:intron 30.0%
TCCTTCAAAAATAATGACTC+CGG - chr1_3:67613715-67613734 None:intergenic 30.0%
TTCTGTGAATGAAGAAGATA+GGG + chr1_3:67613813-67613832 Msa0135850:CDS 30.0%
ATAAACAGCCAAATCAAGTT+TGG - chr1_3:67614103-67614122 None:intergenic 30.0%
TAACATCTTGATCTGATGTT+TGG + chr1_3:67614139-67614158 Msa0135850:intron 30.0%
! ATCTGATGTTTGGAACTTAA+AGG + chr1_3:67614149-67614168 Msa0135850:intron 30.0%
! TGTAAGCTGTATTTCTATGA+CGG - chr1_3:67614451-67614470 None:intergenic 30.0%
TAGATTACTTAACACGCAAA+CGG - chr1_3:67614684-67614703 None:intergenic 30.0%
ACTAAGACTTCAGAACATTT+AGG + chr1_3:67614821-67614840 Msa0135850:intron 30.0%
!!! ATTTTTTCTAAAGGAAGCTG+AGG - chr1_3:67614855-67614874 None:intergenic 30.0%
! TTGATTTTGTGTCTGTTGTT+TGG + chr1_3:67614951-67614970 Msa0135850:intron 30.0%
! GTTTTTGTGCATTCAATTCT+TGG + chr1_3:67615010-67615029 Msa0135850:intron 30.0%
ACATAAACCATAGGATAGAT+GGG - chr1_3:67615088-67615107 None:intergenic 30.0%
AACATAAACCATAGGATAGA+TGG - chr1_3:67615089-67615108 None:intergenic 30.0%
! ATAGTCTTCAGTTTTCAACA+GGG - chr1_3:67615159-67615178 None:intergenic 30.0%
! AATAGTCTTCAGTTTTCAAC+AGG - chr1_3:67615160-67615179 None:intergenic 30.0%
TTTGTGCTTGTATGAAATAG+TGG + chr1_3:67615277-67615296 Msa0135850:intron 30.0%
!!! CTGTTTTTGTTGCAGTATTT+CGG + chr1_3:67615346-67615365 Msa0135850:intron 30.0%
GGGAAAATTGGATTGTGAAA+GGG - chr1_3:67612651-67612670 None:intergenic 35.0%
TGGGAAAATTGGATTGTGAA+AGG - chr1_3:67612652-67612671 None:intergenic 35.0%
GATGCCAAGAATCAAAACAA+TGG + chr1_3:67612785-67612804 Msa0135850:CDS 35.0%
!!! CTTGCCATTGTTTTGATTCT+TGG - chr1_3:67612792-67612811 None:intergenic 35.0%
ACATGAAGGACCAAACTATA+AGG - chr1_3:67613078-67613097 None:intergenic 35.0%
!! TCCTTCATGTGTTGTTTACT+TGG + chr1_3:67613088-67613107 Msa0135850:intron 35.0%
ACCAAGTAAACAACACATGA+AGG - chr1_3:67613092-67613111 None:intergenic 35.0%
TTCTAGGTTTCACTTCTAGA+GGG - chr1_3:67613393-67613412 None:intergenic 35.0%
TTTCTAGGTTTCACTTCTAG+AGG - chr1_3:67613394-67613413 None:intergenic 35.0%
ATTGATACAACACCAACACA+TGG - chr1_3:67613539-67613558 None:intergenic 35.0%
!! TGTTGGTGTTGTATCAATGT+TGG + chr1_3:67613541-67613560 Msa0135850:intron 35.0%
! TTTCAGATTGAAGGTGTGTT+CGG - chr1_3:67613587-67613606 None:intergenic 35.0%
! ACACCTTCAATCTGAAATGT+TGG + chr1_3:67613590-67613609 Msa0135850:intron 35.0%
TTCAAAAATAATGACTCCGG+AGG - chr1_3:67613712-67613731 None:intergenic 35.0%
!!! TCCGGAGTCATTATTTTTGA+AGG + chr1_3:67613711-67613730 Msa0135850:CDS 35.0%
AATCCTCAAGCAATGATGTA+GGG - chr1_3:67613759-67613778 None:intergenic 35.0%
TAATCCTCAAGCAATGATGT+AGG - chr1_3:67613760-67613779 None:intergenic 35.0%
GATTACAAGCAAACCAACTA+TGG + chr1_3:67613775-67613794 Msa0135850:CDS 35.0%
CTTCTGTGAATGAAGAAGAT+AGG + chr1_3:67613812-67613831 Msa0135850:CDS 35.0%
TCTGTGAATGAAGAAGATAG+GGG + chr1_3:67613814-67613833 Msa0135850:CDS 35.0%
!! TTGACACTGTTTTTCTGTTC+GGG - chr1_3:67613843-67613862 None:intergenic 35.0%
!! GTTGACACTGTTTTTCTGTT+CGG - chr1_3:67613844-67613863 None:intergenic 35.0%
TCAAACCACCAAACTTGATT+TGG + chr1_3:67614092-67614111 Msa0135850:intron 35.0%
! TTGGAACTTAAAGGTTCTGA+TGG + chr1_3:67614158-67614177 Msa0135850:intron 35.0%
ATGAGAATAGAGTTCTTCAG+CGG - chr1_3:67614255-67614274 None:intergenic 35.0%
TATTTATCCATTTCAGGGCT+TGG + chr1_3:67614505-67614524 Msa0135850:intron 35.0%
GCTAAACTTCTATTGACACT+TGG + chr1_3:67614536-67614555 Msa0135850:CDS 35.0%
AAACTTCTATTGACACTTGG+AGG + chr1_3:67614539-67614558 Msa0135850:CDS 35.0%
AAAAAAATGCCACGGTTATG+AGG - chr1_3:67614595-67614614 None:intergenic 35.0%
ACAGAACACATTACATTAGC+TGG - chr1_3:67614649-67614668 None:intergenic 35.0%
!! TCTTAGTTTGCCAATTGAGT+TGG - chr1_3:67614808-67614827 None:intergenic 35.0%
! ATTTTCTGTTTGCGTCATGT+TGG + chr1_3:67615195-67615214 Msa0135850:intron 35.0%
TGAAGAATGAATGAGGATTG+AGG - chr1_3:67615246-67615265 None:intergenic 35.0%
CTCAATGTGAAGAATGAATG+AGG - chr1_3:67615253-67615272 None:intergenic 35.0%
!! CTTGTATGAAATAGTGGACA+TGG + chr1_3:67615283-67615302 Msa0135850:intron 35.0%
! CATCTTCTAAGAGTGCATAA+GGG - chr1_3:67615429-67615448 None:intergenic 35.0%
! TCATCTTCTAAGAGTGCATA+AGG - chr1_3:67615430-67615449 None:intergenic 35.0%
ATGCACTCTTAGAAGATGAA+AGG + chr1_3:67615431-67615450 Msa0135850:CDS 35.0%
TGAAAGGGAAAGTTGAGAGA+GGG - chr1_3:67612636-67612655 None:intergenic 40.0%
! TCTAGGTTTCACTTCTAGAG+GGG - chr1_3:67613392-67613411 None:intergenic 40.0%
TTGAATCGCTCACACAAACA+CGG + chr1_3:67613453-67613472 Msa0135850:intron 40.0%
ATTTGAATGTGGCCATGTGT+TGG + chr1_3:67613524-67613543 Msa0135850:intron 40.0%
GCACCAACATTTCAGATTGA+AGG - chr1_3:67613596-67613615 None:intergenic 40.0%
!! GAAATGTTGGTGCTACATGT+AGG + chr1_3:67613603-67613622 Msa0135850:intron 40.0%
TTGAAGGAATTGAAGAGGAG+AGG + chr1_3:67613727-67613746 Msa0135850:CDS 40.0%
ATCCTCAAGCAATGATGTAG+GGG - chr1_3:67613758-67613777 None:intergenic 40.0%
CTCTTCATCAACTCCATAGT+TGG - chr1_3:67613791-67613810 None:intergenic 40.0%
!! CAACTATGGAGTTGATGAAG+AGG + chr1_3:67613789-67613808 Msa0135850:CDS 40.0%
AGTGTCAACCAATGTTGAGA+GGG + chr1_3:67613855-67613874 Msa0135850:CDS 40.0%
GCATTGTTTGTTCTGCTCTA+AGG + chr1_3:67614016-67614035 Msa0135850:intron 40.0%
AACAGCCAAATCAAGTTTGG+TGG - chr1_3:67614100-67614119 None:intergenic 40.0%
TACAGCTTACATGCTTCCTA+TGG + chr1_3:67614460-67614479 Msa0135850:intron 40.0%
!! TGATTTCTGGTGACTACCAT+AGG - chr1_3:67614479-67614498 None:intergenic 40.0%
! GTCAATAGAAGTTTAGCTCG+AGG - chr1_3:67614533-67614552 None:intergenic 40.0%
ACTTGGAGGAACATTCTTCT+TGG + chr1_3:67614553-67614572 Msa0135850:CDS 40.0%
GGAACATTCTTCTTGGCATT+TGG + chr1_3:67614560-67614579 Msa0135850:CDS 40.0%
AAGAGCGGAAAAAAATGCCA+CGG - chr1_3:67614603-67614622 None:intergenic 40.0%
AGAGCTTACGAAGTAAAGAG+CGG - chr1_3:67614618-67614637 None:intergenic 40.0%
TACTTAACACGCAAACGGTA+CGG - chr1_3:67614679-67614698 None:intergenic 40.0%
CAGAACCTAACCAACTCAAT+TGG + chr1_3:67614795-67614814 Msa0135850:intron 40.0%
!! GTTTGCCAATTGAGTTGGTT+AGG - chr1_3:67614803-67614822 None:intergenic 40.0%
TTGTCTTCCCATCTATCCTA+TGG + chr1_3:67615078-67615097 Msa0135850:intron 40.0%
ACTATCACCTCCACAATATG+TGG + chr1_3:67615402-67615421 Msa0135850:CDS 40.0%
GTGAAAGGGAAAGTTGAGAG+AGG - chr1_3:67612637-67612656 None:intergenic 45.0%
TCAACACTTCCAAGTCTTCC+AGG + chr1_3:67612741-67612760 Msa0135850:CDS 45.0%
CAACACTTCCAAGTCTTCCA+GGG + chr1_3:67612742-67612761 Msa0135850:CDS 45.0%
AGAACTACTACAACAGACCC+TGG - chr1_3:67612762-67612781 None:intergenic 45.0%
!! ATTGAAGGTGTGTTCGGTGT+CGG - chr1_3:67613581-67613600 None:intergenic 45.0%
AGCAGTGTGAAGCCATCAAA+AGG - chr1_3:67613661-67613680 None:intergenic 45.0%
!! ATTTTCAGGAGAAGAGCCTC+CGG + chr1_3:67613693-67613712 Msa0135850:intron 45.0%
CAGTGTCAACCAATGTTGAG+AGG + chr1_3:67613854-67613873 Msa0135850:CDS 45.0%
GTGTCAACCAATGTTGAGAG+GGG + chr1_3:67613856-67613875 Msa0135850:CDS 45.0%
GTCTAGAGAATCAGAGGGAA+CGG + chr1_3:67613878-67613897 Msa0135850:CDS 45.0%
TAAGGGTCCACATATTGTGG+AGG - chr1_3:67615412-67615431 None:intergenic 45.0%
GCATAAGGGTCCACATATTG+TGG - chr1_3:67615415-67615434 None:intergenic 45.0%
GGATATCTCAGATAGCTCCT+CGG + chr1_3:67615452-67615471 Msa0135850:CDS 45.0%
!!! TTTTTTATATATATTATTGA+AGG - chr1_3:67613954-67613973 None:intergenic 5.0%
AGGGAAAGTTGAGAGAGGGT+TGG - chr1_3:67612632-67612651 None:intergenic 50.0%
ACAACAGACCCTGGAAGACT+TGG - chr1_3:67612753-67612772 None:intergenic 50.0%
GACCCCTACATCATTGCTTG+AGG + chr1_3:67613753-67613772 Msa0135850:CDS 50.0%
CTCTAGACCCCTCTCAACAT+TGG - chr1_3:67613866-67613885 None:intergenic 50.0%
AGAGGGGTCTAGAGAATCAG+AGG + chr1_3:67613872-67613891 Msa0135850:CDS 50.0%
GAGGGGTCTAGAGAATCAGA+GGG + chr1_3:67613873-67613892 Msa0135850:CDS 50.0%
GAATCAGAGGGAACGGTCTA+TGG + chr1_3:67613885-67613904 Msa0135850:CDS 50.0%
! TCTATGGCGCTGAACAGTGA+AGG + chr1_3:67613901-67613920 Msa0135850:CDS 50.0%
GCTGAACAGTGAAGGTCTTG+AGG + chr1_3:67613909-67613928 Msa0135850:CDS 50.0%
ACCGTTGATCCTCATAACCG+TGG + chr1_3:67614583-67614602 Msa0135850:CDS 50.0%
GCCACGGTTATGAGGATCAA+CGG - chr1_3:67614587-67614606 None:intergenic 50.0%
!! GGACCAAGTTTTGTCCACGA+CGG + chr1_3:67615367-67615386 Msa0135850:CDS 50.0%
CTTCCGTCGTGGACAAAACT+TGG - chr1_3:67615373-67615392 None:intergenic 50.0%
! CGAGGTACCAAGCCCTGAAA+TGG - chr1_3:67614515-67614534 None:intergenic 55.0%
GTGACATCTTGCTTCCGTCG+TGG - chr1_3:67615384-67615403 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 67612614 67615483 67612614 ID=Msa0135850;Name=Msa0135850
chr1_3 mRNA 67612614 67615483 67612614 ID=Msa0135850-mRNA-1;Parent=Msa0135850;Name=Msa0135850-mRNA-1;_AED=0.01;_eAED=0.02;_QI=0|0|0|1|1|1|4|0|217
chr1_3 exon 67612614 67612806 67612614 ID=Msa0135850-mRNA-1:exon:23179;Parent=Msa0135850-mRNA-1
chr1_3 exon 67613701 67613930 67613701 ID=Msa0135850-mRNA-1:exon:23180;Parent=Msa0135850-mRNA-1
chr1_3 exon 67614521 67614628 67614521 ID=Msa0135850-mRNA-1:exon:23181;Parent=Msa0135850-mRNA-1
chr1_3 exon 67615361 67615483 67615361 ID=Msa0135850-mRNA-1:exon:23182;Parent=Msa0135850-mRNA-1
chr1_3 CDS 67612614 67612806 67612614 ID=Msa0135850-mRNA-1:cds;Parent=Msa0135850-mRNA-1
chr1_3 CDS 67613701 67613930 67613701 ID=Msa0135850-mRNA-1:cds;Parent=Msa0135850-mRNA-1
chr1_3 CDS 67614521 67614628 67614521 ID=Msa0135850-mRNA-1:cds;Parent=Msa0135850-mRNA-1
chr1_3 CDS 67615361 67615483 67615361 ID=Msa0135850-mRNA-1:cds;Parent=Msa0135850-mRNA-1
Gene Sequence

>Msa0135850

ATGGCGTTTTTGAGACCAACCCTCTCTCAACTTTCCCTTTCACAATCCAATTTTCCCAGAAATTCTTCATGGAATAATCCAACCCAACAATGTTTTTTTCACAAAAAGATCCTAAAGTTTCAAAATTTCAACACTTCCAAGTCTTCCAGGGTCTGTTGTAGTAGTTCTCAAGATGCCAAGAATCAAAACAATGGAGAAGAGCCTCCGGAGTCATTATTTTTGAAGGAATTGAAGAGGAGAGGTATGACCCCTACATCATTGCTTGAGGATTACAAGCAAACCAACTATGGAGTTGATGAAGAGGCTTCTGTGAATGAAGAAGATAGGGGTTTCCCGAACAGAAAAACAGTGTCAACCAATGTTGAGAGGGGTCTAGAGAATCAGAGGGAACGGTCTATGGCGCTGAACAGTGAAGGTCTTGAGGGCTTGGTACCTCGAGCTAAACTTCTATTGACACTTGGAGGAACATTCTTCTTGGCATTTGGACCGTTGATCCTCATAACCGTGGCATTTTTTTCCGCTCTTTACTTCTATTTCGGACCAAGTTTTGTCCACGACGGAAGCAAGATGTCACTATCACCTCCACAATATGTGGACCCTTATGCACTCTTAGAAGATGAAAGGATATCTCAGATAGCTCCTCGGCTAAATTAA
Protein sequence

>Msa0135850

MAFLRPTLSQLSLSQSNFPRNSSWNNPTQQCFFHKKILKFQNFNTSKSSRVCCSSSQDAKNQNNGEEPPESLFLKELKRRGMTPTSLLEDYKQTNYGVDEEASVNEEDRGFPNRKTVSTNVERGLENQRERSMALNSEGLEGLVPRAKLLLTLGGTFFLAFGPLILITVAFFSALYFYFGPSFVHDGSKMSLSPPQYVDPYALLEDERISQIAPRLN*